Product FAQ

 

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Are immunohistochemistry (IHC) assays developed for formalin-fixed, paraffin-embedded (FFPE) tissues compatible with PAXgene Tissue fixed, paraffin-embedded (PFPE) tissues?
FAQ ID - 3609
Are MaXtract tubes siliconized?
FAQ ID -1298
Are miScript Primer Assay included in miScript miRNA PCR Arrays the same primers as the miScript Primer Assays available in individual tubes?
FAQ ID - 3441

 

Are miScript Primer Assays available for plant mature miRNAs?
FAQ ID - 3439
Are miScript primer assays bench-validated?
FAQ ID -3145
Are Northern Blots sensitive enough to detect siRNA-induced gene silencing?
FAQ ID -403
Are panels and bin sets available for use with GeneMapper ID and GeneMapper ID-X?
FAQ ID - 3267
Are panels and bin sets available for use with GeneMapper ID and GeneMapper ID-X?
FAQ ID -2398
Are pathogens inactivated in human saliva samples collected with the PAXgene Saliva Collector?
3819
Are PAXgene Blood ccfDNA Tubes sterile?
FAQ ID - 3636
Are PAXgene Blood DNA Tubes (IVD) sterile?
FAQ ID - 3373
Are PAXgene Tissue Containers suitable for long term-storage at freezing temperatures?
FAQ ID -3030
Are peak heights of Q48 comparable to other PyroMark instruments?
FAQ ID - 3692
Are plant miScript Primer Assay designs wet-bench validated?
FAQ ID - 3440
Are primers available that only detect mitochondrial DNA encoded genes and not nuclear genomic DNA encoded genes?
FAQ ID -2680
Are probes other than TaqMan® probes, such as FRET probes, compatible with fast cycling with the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2130
Are protein modifications such as glycosylation problematic when using the Qproteome Plasma Membrane Protein Kit?
FAQ ID -1196
Are protocols also available for other instruments (e.g., Hamilton Star and Tecan Fluent)?
3797
Are QIAamp DNA isolation kits suitable for apoptosis studies?
FAQ ID -149
Are QIAcube reagent bottles (cat. no. 990393) autoclavable? What type of plastic are they made of?
FAQ ID - 3369
Are QIAGEN EZ Competent Cells of the QIAGEN PCR Cloning Kit dam+ or dam-?
FAQ ID -638
Are QIAGEN monoclonal Anti His Antibodies glycosylated?
FAQ ID -334
Are QIAprep and QIAquick Spin columns interchangeable?
FAQ ID -311
Are QIAshredder spin columns delivered with the RNeasy Mini QIAcube Kit?
FAQ ID -2333
Are QR codes recognized by QIAsymphony?
FAQ ID - 3625
Are QuantiFast Multiplex PCR Kits available in trial sizes?
FAQ ID -1981
Are QuantiTect PCR +UNG Kits available in bulk format (e.g., 25 ml master mix)?
FAQ ID -2136
Are real-time PCR results obtained with artus® kits quantifiable and how many standards are required?
FAQ ID -1388
Are RNeasy spin columns sold separately?
FAQ ID -159
Are Rotor-Gene Kits compatible with reaction setup using the QIAgility instrument?
FAQ ID -2116
Are run reports and/or log files available on the QIAcube?
148733
Are run reports and/or log files available on the QIAcube?
FAQ ID -1412
Are samples that are contaminated with high molecular weight DNA (e.g. from a paramedic or police officer) a problem with REPLI-g amplification?
FAQ ID -683
Are scoring sheets available for each crystallization condition Screening Suite?
FAQ ID -1125
Are special kits and protocols required to isolate biomolecules from PAXgene Tissue fixed, cryo-embedded (PFCE) tissue?
FAQ ID - 3614
Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with QuantiFast Probe PCR Kits?
FAQ ID -1452
Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with Rotor-Gene Probe Kits?
FAQ ID -2126
Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2131
Are the 96- and 384-sample blocks interchangeable?
153924
Are the assays also compatible with qPCR?
FAQ ID - 3871
Are the assays species-specific?
FAQ ID — 3400
Are the BioSprint 15/96 instruments stand-alone workstations?
FAQ ID -620
Are the buffers in the Ni-NTA Fast Start Kit the same as the ones for use with Ni-NTA purchased separately?
FAQ ID -791
Are the buffers in the RNeasy QIAcube kit delivered in QIAcube buffer bottles?
FAQ ID -2334
Are the CGT dPCR assays also compatible with qPCR?
FAQ ID - 3981

Are the columns of the MinElute Reaction Cleanup-, Gel Extraction-, and PCR Purification Kit identical?
FAQ ID -581
Are the columns of the QIAquick PCR Purification-, Gel Extraction-, and Nucleotide Removal Kit interchangeable?
FAQ ID -577
Are the CoralLoad dyes in the HotStarTaq Plus PCR Buffer visible when loading small amounts of PCR product onto a gel?
FAQ ID -1051
Are the CRISPR-Q PCR Assays and the CRISPR-Q Sanger Primers on GeneGlobe validated?
3793
Are the Internal Controls used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit homologous to a known target?
FAQ ID -2598
Are the layouts for both 96- and 384-well QuantiTect Primer Assay Plates visible when ordering online?
FAQ ID -2111
Are the Microbial DNA qPCR Assays wet-lab verified?
FAQ ID — 3406
Are the miRNA Target Protector & miRNA mimic bench tested? Any guarantee?
FAQ ID - 3186
Are the multicopy reference assays for Type-it CNV PCR +qC Kits and the qBiomarker assays the same?
FAQ ID -3000
Are the new EZ1 RNA Kits (cat. no. 958034; 959034; 956034) compatible with the previous EZ1 RNA Card (cat. no. 9015590) or EZ1 RNA Universal Tissue Card (cat. no 9016384)?
FAQ ID -1025
Are the PAXgene Blood DNA Tubes sterile?
FAQ ID - 3487
Are the PCR products generated with CRISPR-Q Custom PCR Assays only applicable to analysis of editing efficiency by Sanger sequencing?
3794
Are the primer sets contained in the Investigator IDplex kit the same as in competitor kits?
FAQ ID -2407
Are the primer sets different for the Investigator Nonaplex ESS Kit and ESSplex SE where the loci are the same?
FAQ ID -2399
Are the primer sets different for the Investigator Nonaplex ESS Kit and Investigator Hexaplex ESS Kit where the loci are the same?
FAQ ID -2400
Are the protocols from the BioSprint software compatible with the KingFisher software and vice versa?
FAQ ID -770
Are the QIAprecipitator Midi and Maxi Modules of the HiSpeed Plasmid Kits interchangeable?
FAQ ID -308
Are the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit suited for short hairpin vectors?
FAQ ID -2318
Are the QIAseq miRNA NGS 5’ Adapter and QIAseq miRNA NGS RT Initiator compatible with the QIAseq miRNA UDI kits?
FAQ ID - 3836
Are the QIAshredder columns included in various QIAGEN kits the same?
FAQ ID - 3624
Are the QIAwave kits based on a different chemistry than the legacy kits?
FAQ ID - 3990

Are the QuantiFast Probe Assays hydrolysis or FRET probes?
FAQ ID - 3271
Are the QuantiFast Probe Assays hydrolysis or FRET probes?
FAQ ID -2363
Are the QuantiFast Probe Assays wet-lab validated?
FAQ ID -2375
Are there any accessories required for sample disruption with the TissueLyser LT?
FAQ ID -2551
Are there any changes in the procedure using the Strep-Tactin Superflow Plus resin with the higher capacity over the standard matrix?
FAQ ID -2168
Are there any general recommendations to take into account when performing a nucleic acid extraction?
FAQ ID -1494
Are there any special considerations for the collection of blood using PAXgene Blood DNA Tubes (IVD) (e.g., use of a blood collection set or discard tube, positioning of the tube, issues with short draws, etc.)?
FAQ ID - 3371
Are there any specific cleaning solutions recommended for the QIAcube?
FAQ ID -1416
Are there any specific cleaning solutions recommended for the QIAsymphony SP?
FAQ ID -1913
Are there any unique elements that need to be incorporated into an shRNA expression vector?
FAQ ID -2772
Are there components in biological samples that can inhibit the REPLI-g reaction?
FAQ ID -667
Are there important considerations for plasma generation and urine handling?
FAQ ID - 3699
Are there other options for paraffin removal?
3754
Are there other options for paraffin removal?
FAQ ID - 3949
Are there other options for paraffin removal?
FAQ ID - 3946
Are there problems with the default threshold setting on the Applied Biosystems 7500 when using QuantiFast Probe PCR Kits?
FAQ ID -1702
Are there recommendations for sensitive detection of underrepresented fragments using the Type-it Mutation Detect PCR Kit?
FAQ ID -2066
Are there special considerations for phlebotomy when using the PAXgene Blood ccfDNA Tube (blood collection set, discard tube, positioning of the tube, issues with short draws, etc.)?
FAQ ID - 3635
Are there special requirements for flow sorting?
FAQ ID - 3546
Are there special requirements for flow sorting?
FAQ ID - 3558
Are there specific recommendations for performing RT-PCR on RNA isolated from paraffin-embedded samples?
FAQ ID -828
Are there specific recommendations for performing RT-PCR on RNA isolated from paraffin-embedded samples?
FAQ ID - 3955
Are there specific recommendations for performing RT-PCR on RNA isolated from paraffin-embedded samples?
FAQ ID - 3944
Are there stopping points in the Hi-C workflow?
FAQ ID - 143071
Are there two vectors for protein expression using QIAgenes in insect or mammalian cells?
FAQ ID -2048
Are we able to customize protocol on the EZ2 Connect?
FAQ ID - 3850
Are you able to supply a Certificate of Analysis for the KRAS and EGFR PCR kits?
FAQ ID -2301
Are your FlexiTube siRNAs preferentially designed to any particular region of the target gene?
FAQ ID -1091
As part of my RT procedure I routinely heat an aliquot of the eluate prior to the RT reaction. Do I still need to heat the whole eluate after the RNA preparation?
FAQ ID - 3468
Aside from the OTV test, what other maintenance should be done for the Rotor-Gene Q?
FAQ ID -3181
At what point can I stop and freeze my samples when using ChIP?
FAQ ID -2754
At what temperature can I perform the experiment with the NexTal Cubic Phase kits?
FAQ ID -2323
At what temperature can I perform the NexTal Cubic Phase experiment and incubate the plate?
FAQ ID -2415
At which step in the REPLI-g FFPE protocol should PicoGreen quantification be done?
FAQ ID -1758
Buffer SB1 in the GeneRead Size Selection Kit turned yellowish from a new kit. Can I still use it?
FAQ ID - 3562
Can 0.1 ml and 0.2 ml tubes used on the Rotor-Gene Q be labeled?
FAQ ID -2082
Can 2 µl reaction volumes be used with QuantiFast PCR Kits?
FAQ ID -1440
Can 4-plex, real-time PCR be performed on the ABI PRISM 7000, 7700, or 7900 using the QuantiFast Multiplex PCR Kit?
FAQ ID -1978
Can a phenol-chloroform ratio different from 1:1 also be used with MaXtract Low and High Density?
FAQ ID -1309
Can a QIAquick Gel Extraction Kit be used to obtain RNA from a formaldehyde gel?
FAQ ID -133
Can acetone be used for precipitation of protein from Buffer RLT lysates generated with RNeasy Kits?
FAQ ID -1164
Can additional positive controls be used for the Investigator IDplex GO! Kit?
FAQ ID -3094
Can Allprotect Tissue Reagent be used to stabilize RNA, DNA and proteins in cells, bacteria, yeast, and plants?
FAQ ID -1574
Can amplification with the QuantiTect Whole Transcriptome Kit be extended to more than 8 hours to improve cDNA yields?
FAQ ID -1608
Can assays for 20 and 100 reactions be ordered on the same QuantiTect Primer Assay Plate?
FAQ ID -2108
Can BAC DNA prepared with the R.E.A.L. Prep 96 Plasmid Kit be used for high-throughput sequencing?
FAQ ID -643
Can BioMag be used for positive and negative selection?
FAQ ID -269
Can BioSprint 96 be connected to LIMS?
FAQ ID -3070
Can blood samples be frozen in PAXgene Blood DNA Tubes (IVD)?
FAQ ID - 3377

 

Can bone marrow aliquots other than 2 mL be pipetted into the PAXgene Bone Marrow RNA Tube?
FAQ ID -2503
Can bone marrow from leukemia patients with elevated numbers of white blood cells be processed with the PAXgene Bone Marrow RNA System?
FAQ ID -2510
Can Buffer R1 of the R.E.A.L. Prep 96 Plasmid Kit be replaced with Buffer P1?
FAQ ID -823
Can Buffers N3 and P3 be used interchangeably?
FAQ ID -310
Can cDNA prepared by any method be used as starting material in the ligation reaction of the QuantiTect Whole Transcriptome Kit?
FAQ ID -1589
Can cDNA prepared with the QuantiTect Whole Transcriptome Kit be reampliied using REPLI-g Kits?
FAQ ID -1611
Can cDNA synthesized with the miScript Plant RT Kit be used in real-time PCR with TaqMan® MicroRNA Assays?
FAQ ID - 3445
Can cells stabilized with RNAprotect Cell Reagent be used for cell sorting by flow cytometry (FACS)?
FAQ ID -1216
Can customers use their own DNA size marker with the QIAxcel System and if yes, what concentration should be used?
FAQ ID -1841
Can data be analyzed on the Rotor-Gene Q while a run is in progress?
FAQ ID -2091
Can data be analyzed while the run is proceeding? Can data be analyzed from a previous experiment while another experiment is running?
153948
Can data be saved at once with the QIAxcel System after analysis of all samples in a 96-well PCR plate?
FAQ ID -1861
Can degraded RNA be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1586
Can different amounts of siRNA be ordered on one FlexiPlate?
FAQ ID -1473
Can different incubation times be used for the 2 hour amplification step in the QuantiTect Whole Transcriptome protocol?
-8
Can Dithiothreitol (DTT) be used instead of BME in Buffer RLT Plus of RNeasy Plus Mini Kit?
FAQ ID -3125
Can DNA isolated with MaXtract tubes be used for downstream applications such as restriction digestion, labeling, blotting, PCR, and automated sequencing?
FAQ ID -1303
Can DNA yields be increased by prolonging incubation with proteinase K in Buffer ATL?
FAQ ID -2032
Can DNase digestion be performed on the RNeasy 96 plate during the RNA purification part of the Allprep DNA/RNA 96 procedure?
FAQ ID -2000
Can DyeEx be used to clean up labeled DNA other than sequencing templates?
FAQ ID -137
Can fast cycling be carried out with QuantiFast Probe PCR Kits and FRET probes?
FAQ ID -1433
Can FlexiPlate siRNA be ordered in solution on dry-ice?
FAQ ID -1474
Can FlexiPlate siRNAs be ordered in plates other than the standard NUNC 96 well V bottom plates?
FAQ ID -1458
Can FlexiTube siRNA or FlexiTube GeneSolution products be ordered in solution?
FAQ ID -1667
Can frozen blood be used for the procedure?
3832
Can frozen blood be used for whole genome amplification with REPLI-g?
FAQ ID -794
Can frozen sample material pulverized in liquid nitrogen be directly added to MaXtract tubes?
FAQ ID -1301
Can frozen samples be used with the MagAttract 96 cador Pathogen Kit?
FAQ ID -2070
Can FTA paper for dried blood spots be used with REPLI-g?
FAQ ID -685
Can Glutathione Superflow resin be reused?
FAQ ID -2171
Can glycosylated proteins be expressed using the EasyXpress Insect Kit II?
FAQ ID -1227
Can Heparin blood be used?
3834
Can HiPerFect HTS be used for miRNA?
FAQ ID -2561
Can HiPerFect HTS Reagent be used for transfecting shRNAs?
FAQ ID -2559
Can HP Validated siRNAs be ordered on the FlexiPlate?
FAQ ID -1367
Can I add bleach to the sample preparation waste?
FAQ ID - 3505
Can I adjust the ROX concentration in the QuantiFast master mix?
FAQ ID -1427
Can I also screen large constructs like BACs, fosmids, and cosmids using the QIAGEN PlasmidAmp Kit?
FAQ ID -1337
Can I amplify from plasma or serum samples using the REPLI-g Mini/Midi kits?
FAQ ID -671
Can I amplify mitochondrial DNA directly from whole blood or cells using the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1319
Can I analyze my Next Generation Sequencing (NGS) samples on the QIAxcel for Quality control?
FAQ ID - 3724
Can I ask QIAGEN to upgrade my current QIAcube Connect Life Science instrument to make it an MDx instrument?
148744
Can I buy QIAquick and MinElute columns separately?
FAQ ID -2460
Can I buy the 1 ml Bead Plates from the DNeasy UltraClean 96 Microbial Kit (384) separately?
FAQ ID - 3726
Can I buy the gDNA eliminator columns supplied in your RNeasy Plus kits separately?
FAQ ID - 3390
Can I buy the gDNA eliminator plates supplied in your RNeasy Plus 96 kit separately?
FAQ ID - 3389
Can I buy the RNeasy Mini columns, RNeasy MinElute columns, RNeasy Midi columns or the RNeasy Maxi columns separately?
FAQ ID - 3388
Can I change the language on my QIAsymphony SP system?
FAQ ID - 3595
Can I clean up my DNase treated RNA samples using RNeasy columns?
FAQ ID -286
Can I combine your CGT dPCR assays with custom designed assays?
FAQ ID - 3861
Can I connect more than one QIAquant instrument to the same computer?
153953
Can I directly transfect SureSilencing shRNA Plasmids into my cells?
FAQ ID -2899
Can I do 4-plex real-time PCR, on the ABI PRISM 7000, 7700, or 7900 using the QuantiTect Multiplex PCR Kit?
FAQ ID -716
Can I do singleplex PCR with the QuantiFast Probe Assays?
FAQ ID -2362
Can I do whole genome amplification from brain tissue using REPLI-g?
FAQ ID -669
Can I do whole genome amplification from mitochondrial DNA using REPLI-g?
FAQ ID -668
Can I eliminate RNase A from buffer P1 for my plasmid preparation to obtain RNase-free DNA for in-vitro transcription?
FAQ ID -366
Can I follow the reaction setup for probe-based assays described in Appendix D of the FastLane Cell RT-PCR Handbook using 384-well plates?
FAQ ID -1482
Can I get IQ/OQ documents from QIAGEN so that I can perform my own IQ/OQ?
FAQ ID -1558
Can I homogenize samples in QIAzol using the QIAshredder?
FAQ ID -501
Can I increase the annealing temperature recommended for QuantiTect Primer Assays used with the QuantiTect SYBR Green PCR Kits?
FAQ ID -1146
Can I make a master mix with QuantiProbes, primers, and all other reaction components and store it for later use?
FAQ ID -584
Can I make stable cell lines with the Cignal Reporter Assays?
FAQ ID -2763
Can I manually set the threshold line?
FAQ ID -2702
Can I measure antibiotic resistance gene expression?
FAQ ID — 3404
Can I measure virulence factor gene expression?
FAQ ID — 3405
Can I multiplex the Microbial DNA qPCR Assays?
FAQ ID — 3408
Can I obtain the sequence of the RT² qPCR Primer Assay that I purchased?
FAQ ID -2709
Can I operate QIAquant 96 instrument using touchscreen and computer simultaneously?
153951
Can I optimize a dPCR assay on the QIAcuity using gradient temperature?
FAQ ID - 3783
Can I order Alexa Fluor labeled controls with FlexiPlate siRNA?
FAQ ID -1369
Can I order the nucleotides from PyroMark Gold Reagents separately?
FAQ ID -2827
Can I perform in-vitro transcription with the pDrive Cloning Vector from the QIAGEN PCR Cloning Kit?
FAQ ID -332
Can I perform the IDH 1/2 RGQ PCR test with slides containing less than 40% tumor cells?
FAQ ID -3098
Can I prepare a dPCR reaction directly in QIAcuity Nanoplate?
3774
Can I program my own protocols for the QIAcube Connect MDx?
148727
Can I program my own protocols for the QIAcube Connect?
FAQ ID - 141486
Can I program my own protocols for the QIAcube?
FAQ ID -1405
Can I program my own protocols for the QIAsymphony SP?
FAQ ID -1904
Can I purchase Phi29 DNA polymerase only?
FAQ ID -708
Can I re-amplify whole genome amplification product?
FAQ ID -670
Can I reclone the insert sequences from the SureSilencing shRNA Plasmids into a viral-based vector?
FAQ ID -2896
Can I reduce the amount of starting material below 10 ng when using the REPLI-g UltraFast Mini Kit?
FAQ ID -1325
Can I reduce the reaction time to e.g., 30 minutes, when using the REPLI-g UltraFast Mini Kit?
FAQ ID -1326
Can I reinstall the PyroMark Q24 software on my new computer or following an operating system upgrade, or do I need to purchase a new license?

FAQ ID - 3473

Can I reliably detect intermediately methylated DNA with EpiTect Methyl qPCR Array technology?
FAQ ID -2738
Can I reuse the Ni-NTA Agarose and Ni-NTA Superflow resins?
FAQ ID -802
Can I run less than 15 samples on the BioSprint 15 instrument?
FAQ ID -621
Can I run life science and custom protocols on a QIAcube Connect MDx?
148745
Can I run RUO protocols on the new or upgraded BioRobot EZ1 DSP?
FAQ ID -1563
Can I scale up reagents if I want to use more sample with the MagAttract HMW DNA Kit (cat. no. 67563)?
FAQ ID - 3448
Can I see error codes on the instrument touchscreen?
3763
Can I see on the QIAcuity Software Suite report file if the VPF (Volume Precision Factor) has been used or not?
3770
Can I select FITC labeled cells with BioMag?
FAQ ID -278
Can I select HP Validated and HP GenomeWide siRNAs for FlexiTube?
FAQ ID -1658
Can I shorten the activation time for the HotStarTaq DNA Polymerase?
FAQ ID -565
Can I stabilize adipose tissue using RNAprotect Tissue Reagent prior to RNA isolation?
FAQ ID -856
Can I stabilize RNA in bacteria grown on solid media or substrate using the RNAprotect Bacteria Reagent?
FAQ ID -615
Can I still perform sequencing if my miRNA sequencing library concentrations are too low to obtain a 4 nM library?
FAQ ID - 3666
Can I still reorder siRNA that has been removed from the GeneGlobe data base?
FAQ ID -1668
Can I stop saliva collection before the fill line is reached?
3806
Can I store agarose gel slices containing DNA for gel extraction at a later point?
FAQ ID -313
Can I store cDNA generated by the miScript System?
FAQ ID -1602
Can I store the QIAseq Beads of the QIAseq Targeted DNA Panel at -20°C?
FAQ ID - 3711
Can I substitute the endonuclease used in the Hi-C Digestion step with another endonuclease?
FAQ ID - 143070
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