Product FAQ

 

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Why do I sometimes get light blue colonies when using the QIAGEN PCR Cloning Kit?
FAQ ID -603
Why do I see multiple high-intensity peaks in my qPCR dissociation curve at temperatures less than 70 ºC?
FAQ ID -2690
Why do I see low, poor, or sub-standard amplification efficiency in my qRT-PCR assay?
FAQ ID -2695
Why do I observe a CT shift for the Internal Control in some samples in comparison to the CT value for the Internal Control of the dilution series of the Male Control DNA M1?
FAQ ID - 3745
Why do I need to identify my real-time instrument model when placing my order for RT² qPCR Primer Assays?
FAQ ID -2713
Why do I need normalization using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2198
Why do I have wavy DNA bands on my agarose gel?
FAQ ID -2260
Why do I have to use an ACS (Assay Control Set)?
FAQ ID -2933
Why do I have to add beta-mercaptoethanol (beta-ME) to lysis Buffer RLT of the RNeasy Kits?
FAQ ID -101
Why do I get smeared PCR products?
FAQ ID -87
Why do I get genomic DNA contamination in my plasmid prep?
FAQ ID -353
Why do I get amplification in a negative control DNA tube using the REPLI-g Kit for WGA?
FAQ ID -675
Why do casework-sample protocols for the QIAsymphony DNA Investigator Kit start with different lysate volumes (200 µl, 500 µl, or 1000 µl)?
FAQ ID -2035
Why did you choose ORF1a+ORF1b and ORF3a+ORF7a?
FAQ ID - 3893
Why did you choose MAPK1 as a positive control in the QIAGEN RNAi Human/Mouse Control Kit?
FAQ ID -604
Why did we not choose commonly used genes such as the S gene, E gene, or RdRP?
FAQ ID - 3892
Why did the real-time PCR yield Ct values < 12?
FAQ ID -2727
Why can’t I see my QIAcube Connect instrument in the QIAcube Connect App?
FAQ ID - 1414518
Why can't I find the DNA methylation qPCR Assay for my gene of interest?
FAQ ID -2743
Why can I not find the QuantiTect Primer Assay in GeneGlobe that I had previously ordered from QIAGEN?
FAQ ID -1138
Why can I not find the Q-Base device in the device list during the first installation?
FAQ ID - 141516
Why are two reagents used in the PAXgene Tissue System?
FAQ ID -3022
Why are two reagents used in the PAXgene Tissue System?
FAQ ID - 3603
Why are there various different QuantiTect Primer Assays for my gene (previous versions, transcript variants)?
FAQ ID -1133
Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)?
FAQ ID -2366
Why are the RT² qPCR Primer Assays not designed to cross exon-intron junctions or boundaries?
FAQ ID -2710
Why are the QuantiTect and QuantiFast Multiplex PCR Kits limited to triplex real-time PCR on some cyclers?
FAQ ID -715
Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA?
FAQ ID -2365
Why are the denaturation and annealing/extension times for QuantiFast Multiplex RT-PCR Kits much shorter than those for QuantiFast Multiplex PCR Kits?
FAQ ID -2147
Why are the centrifugation speeds for the QIAamp DNA Mini kit at 6000 x g? Can I spin at full speed?
FAQ ID -474
Why are some of my samples outside of the cluster using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2204
Why are some of my RNAprotect-stabilized samples frozen at 0ºC while others are not?
FAQ ID -758
Why are samples centrifuged at 4°C after the addition of chloroform when using RNeasy Lipid Tissue Kits?
FAQ ID -533
Why are samples centrifuged at 4°C after addition of chloroform to the QIAzol lysates in following kits: RNeasy Lipid Tissue Mini/Midi kits, RNeasy Microarray Tissue Mini kit, RNeasy Plus Universal Mini/Midi kit, RNeasy 96 Universal Tissue kit, miRNeasy Mini kit, miRNeasy Micro kit, miRNeasy 96 kit and miRNeasy Serum/Plasma kit?
FAQ ID -2345
Why are not all applications/kits/protocol files visible in the run setup (QIAcube Connect App)?
FAQ ID - 1414520
Why are my realtime PCR amplification plots hook-shaped?
FAQ ID -587
Why are my qPCR Ct values too low (< 12) in my qRT-PCR Assay?
FAQ ID -2684
Why are my qPCR Ct values too high (> 35 or not detectable) in my qRT-PCR assay?
FAQ ID -2685
Why are my DV200 values of my RNA sample so low?
FAQ ID - 3957
Why are most of my samples outside of the cluster in supervised mode using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2205
Who can help in case of issues with the third party instrument?
3799
Who can help if it is not clear if issues are caused by the third party instrument or by the chemistry?
3802
Which version of the EZ2 Connect can run the EZ2 RNA/miRNA Tissue/Cells Kit?
FAQ ID - 3851
Which version of the EZ2 Connect can run the EZ2 RNA FFPE Kit?
FAQ ID - 3940
Which version of the EZ2 Connect can run the EZ2 AllPrep DNA/RNA FFPE Kit?
FAQ ID - 3947
Which variants of the EasyXtal 15-well tool are available?
FAQ ID -2221
Which types and amounts of sample material can be processed for RNA extraction on the QIAsymphony SP?
FAQ ID -1937
Which tubes are recommended for the QIAsymphony sample rack
FAQ ID - 3653
Which transfection reagent is recommended for transfection of the miScript Target Protectors?
FAQ ID -2252
Which software updates are included in a Premium Cover Agreement for my QIAGEN Instrument?
FAQ ID -1336
Which SARS-CoV-2 targets can the SARS-CoV-2 Neo Kit detect?
FAQ ID - 3881
Which sample volumes can be used for the QIAsymphony DSP Circulating DNA Kit?
FAQ ID - 3700
Which sample types can be processed using the QIAsymphony Virus Blood 200 protocol?
FAQ ID -2154
Which sample types can be processed using the QIAsymphony PAXgene Blood RNA Protocol?
FAQ ID -2981
Which sample types can be processed using the PAXgene Blood miRNA protocols?
FAQ ID - 3478
Which sample materials are CE-IVD validated for the RealStar Filovirus Screen?
FAQ ID - 3529
Which Rotor-Gene Q rotor should be used with the EGFR RGQ PCR kit?
FAQ ID -2315
Which resin is used in the QIAexpress Ni-NTA Fast Start Columns?
FAQ ID -836
Which regions are covered by the GR DNAseq V2 Panels (#181900)?
FAQ ID - 3688
Which real-time PCR kits are recommended downstream of the QuantiTect Whole Transcriptome Kit?
FAQ ID -1592
Which QuantiTect Primer Assay should I choose for my gene of interest?
FAQ ID -1135
Which Qproteome or Protein Fractionation Kits from QIAGEN are compatible with tissue samples?
FAQ ID -755
Which qPCR instrument should I use with your RT² qPCR Primer Assays?
FAQ ID -2714
Which QIAsymphony Virus/Bacteria Kit size should be used for each protocol?
FAQ ID -2038
Which QIAGEN plasmid preparation kits will contain LyseBlue Reagent?
FAQ ID -865
Which QIAGEN kit do you recommend for purifying plasmid DNA suitable for transfection of sensitive cells?
FAQ ID -1092
Which QIAGEN DNA extraction kits are compatible with the PAXgene Saliva Collector?
3821
Which QIAcube Connect standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet?
FAQ ID - 141508
Which PyroMark Gold Q96 Reagent should be used for which instrument and application?
FAQ ID -2836
Which purity grade is recommended for pyrosequencing primers?
FAQ ID -2832
Which purification kits are recommended for the Pyro kits?
FAQ ID -2380
Which protocol line should I use to process plasma samples?
FAQ ID - 3654
Which probe labels are available for the QuantiFast Probe Assays?
FAQ ID -2361
Which probe labels are available for the Microbial DNA qPCR Assays?
FAQ ID — 3407
Which primers can I use for sequencing pQE-expression vector constructs?
FAQ ID -343
Which positive control should I use?
FAQ ID - 3896
Which plate formats are offered for QuantiTect Primer Assay Plates?
FAQ ID -2106
Which plasma volumes can I process with the QIAsymphony PAXgene Blood ccfDNA protocols?
FAQ ID - 3648
Which parts are exchanged during a Preventive Maintenance visit for my QIAGEN BioRobot?
FAQ ID -1334
Which operating system is compatible with PyroMark Q24 Advanced application software?
FAQ ID -3164
Which operating system is compatible with PyroMark IdentiFire Software?
FAQ ID - 3340
Which nucleotide analogs can be used with TopTaq DNA Polymerase?
FAQ ID -1741
Which network settings are supported by the QIAcube Connect and Q-Base?
FAQ ID - 141517
Which mode should I use in the Rotor-Gene ScreenClust HRM Software, supervised or unsupervised?
FAQ ID -2202
Which Microbial qPCR Mastermix should I use?
FAQ ID — 3395
Which materials can be used as input samples for the QIAsymphony DSP Circulating DNA Kit?
FAQ ID - 3702
Which kits can be used in combination with the PyroMark CpG Assays and PyroMark Custom Assays?
FAQ ID -2822
Which kits and protocols can be used to isolate nucleic acids from microdissected PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) specimens?
FAQ ID - 3617
Which kit, the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, do you recommend for transfection?
FAQ ID -2320
Which kit size should be used for each QIAsymphony DSP Virus/Pathogen protocol?
FAQ ID -2930
Which kit should I use for RNA isolation from Cartilage?
FAQ ID -1026
Which kit should be used to extract RNA from adipose tissue, brain, and other fatty animal tissues?
FAQ ID -467
Which kit can be used for simultaneous purification of DNA and RNA from biopsies?
FAQ ID -1089
Which kind of 96 and 384-well plates are available for FlexiPlate siRNAs?
FAQ ID -1459
Which is the best condition for eluting GST-tagged proteins from Glutathione Superflow?
FAQ ID -2173
Which human target is used for the quantification in the Investigator Quantiplex Kit?
FAQ ID -2577
Which homogenization method do you recommend for tissues stored in Allprotect Tissue Reagent?
FAQ ID -1572
Which heating block is recommended for the pyrosequencing annealing step?
FAQ ID -2838
Which fraction will contain soluble mitochondrial proteins using the Qproteome Cell Compartment Kit?
FAQ ID -839
Which fraction will contain endosomal, microsomal and lysosomal proteins using the Qproteome Cell Compartment Kit?
FAQ ID -808
Which formats are available for FlexiPlate siRNA?
FAQ ID -1358
Which fixation method is used in the PAXgene Tissue System?
FAQ ID - 3600
Which extraction kits are recommended?
FAQ ID - 3874
Which expression systems can be used with the QIAgenes Insect/Mammalia expression constructs?
FAQ ID -2049
Which EpiTect Bisulfite Plus kit do you recommend for blood samples, cells, and tissues?
FAQ ID -2411
Which end of the PCR primer for pyrosequencing should be biotinylated?
FAQ ID -2839
Which elution volumes can be used for the QIAsymphony DSP Virus/Pathogen protocols?
FAQ ID -2938
Which elution volumes can be selected using the QIAsymphony Virus Blood 200 protocol?
FAQ ID -2160
Which elution volumes are available with the QIAsymphony PAXgene Blood ccfDNA Kit?
FAQ ID - 3655
Which elution volume is used for the QIAsymphony DSP Circulating DNA Kit?
FAQ ID - 3703
Which downstream applications have been tested with SARS-CoV-2-derived RNA purified from saliva collected into PAXgene Saliva Collector?
3828
Which downstream applications have been tested with DNA purified from samples collected in PAXgene Blood DNA Tubes IVD?
FAQ ID - 3512
Which downstream applications have been tested with DNA purified from saliva collected into PAXgene Saliva Collector?
3827
Which downstream applications have been tested with ccfDNA purified from whole blood collected and processed with the PAXgene Blood ccfDNA System?
FAQ ID - 3658
Which DNA/RNA extraction kits were tested in combination with the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2606
Which deparaffinization method can I use with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2352
Which density gel type is contained in the yellow or green MaXtract Tubes?
FAQ ID -1315
Which database versions are used for CRISPR-Q Custom PCR Assay and CRISPR-Q Sanger Primers design?
3788
Which crystallization solutions are compatible with lipidic cubic phase crystallization?
FAQ ID -2418
Which controls can I choose for FlexiPlate siRNA?
FAQ ID -1368
Which conditions are recommended for the storage of tissues in PAXgene Tissue STABILIZER?
FAQ ID - 3605
Which competent E. coli cells should I use to amplify the SureSilencing shRNA Plasmids?
FAQ ID -2890
Which chloroform should I use for the RNeasy Lipid Tissue Kit?
FAQ ID -516
Which chemical products do you use to prepare screening solutions for protein crystallization?
FAQ ID -1122
Which cell lines have been tested with FastLane Cell RT-PCR Kits?
FAQ ID -1485
Which cell collection methods are compatible with REPLI-g Single Cell RNA Library Kit?
FAQ ID - 3555
Which cell collection methods are compatible with REPLI-g Single Cell DNA Library Kit?
FAQ ID - 3543
Which capillary sequencers can be used for detection of amplicons from the Type-it Microsatellite PCR Kits?
FAQ ID -2063
Which barcodes on sample tubes are accepted by the QIAsymphony SP?
FAQ ID - 3598
Which Anti-His Antibody is the most sensitive for my protein of interest?
FAQ ID -172
Which analyses can be performed with the PyroMark Q96 MD software?
FAQ ID -2866
Which analyses can be performed with the PyroMark Q96 ID software version 1.0?
FAQ ID -2845
Where to look for ordering Custom siRNAs online?
FAQ ID -3106
Where should I add a dye-label modification on the siRNA?
FAQ ID -648
Where can I order the Streptavidin Sepharose beads for pyrosequencing?
FAQ ID -2850
Where can I find the serial number on QIAGEN's BioRobots?
FAQ ID -1064
Where can I find the public Smith-Waterman homology search tool that you refer to on your siRNA online design page?
FAQ ID -562
Where can I find the ordering information for QIAcube Connect accessories such as shaker rack plugs, rack labeling strips, reagent bottle racks, 30 ml reagent bottles, rotor-adapter holder, rotor adapters, sample tubes CB, sample tubes RB and spin-column adapter rings?
FAQ ID - 141505
Where can I find the ordering information for QIAcube accessories such as Shaker Rack plugs, Rack labeling strips, Reagent bottle rack, 30mL Reagent bottles, Rotor Adapter holder, Rotor adapters, Sample tubes CB, Sample tubes RB and Spin column adapter rings?
FAQ ID -3054
Where can I find the gene list for a specific RT² Profiler PCR Array?
FAQ ID - 3684
Where can I find the composition tables for solutions used in individual Crystallization Screening Suites?
FAQ ID -1124
Where can I find the certificate of calibration for the Rotor-Disc OTV Kit (catalog number 981400)?
FAQ ID - 3695
Where can I find QIAGEN products for a specific gene or gene product?
FAQ ID -803
Where can I find information about the worktable setup on the BioRobot EZ1?
FAQ ID -1243
Where can I find info on compatible reporter dyes for use in real-time multiplex PCR using the QuantiTect Multiplex PCR Kits?
FAQ ID -719
Where can I find general information about RT-PCR and PCR arrays such as information about sample preparation, reverse transcription, quantitative PCR, real-time PCR, and PCR arrays?
FAQ ID -2446
Where can I find explanations to the warning given by the PyroMark software after run data analysis?
FAQ ID -2874
Where can I find background information and literature on Whole Genome Amplification with REPLI-g Kits?
FAQ ID -1690
Where can I find additional information for PreAnalytiX PAXgene products?
FAQ ID - 3515
Where can I find a protocol for cleanup of already purified plasmid DNA?
FAQ ID -1031
Where can I find a copy of the report files generated by running accessory protocols "Dilutor Delivery (monthly)" and "High-Speed Pipetting Sytem (monthly)" on my BioRobot 9604?
FAQ ID -766
Where can I find a copy of the report file generated by running 'Maintenance Protocol I' on my BioRobot MDx?
FAQ ID -806
Where can I find a copy of the .kpf file for a BioSprint protocol?
FAQ ID -3073
Where can I download the protocol for the KingFisher Flex?
3798
Where can I access Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit?
FAQ ID -3085
Where are the somatic mutations published?
FAQ ID -2429
When would I use the mericon DNA Bacteria Kit vs the mericon DNA Bacteria Plus Kit?
FAQ ID - 3348
When using the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit, what should the fluorescent label of the probe be for the customer-defined assay to detect the pathogen?
FAQ ID -2594
When using QIAzol Lysis Reagent for RNA extraction, I see a pinkish colored aqueous phase! Is it OK?
FAQ ID -534
When using HiPerFect HTS Reagent is it necessary to optimize amount of siRNA and transfection reagent?
FAQ ID -2556
When should the Normalization protocol be used?
FAQ ID -1155
When should I use the ‘Standard’ vs. the ‘Fast’ protocol for the EZ2 AllPrep DNA/RNA Kit
FAQ ID - 3948
When should I use the standard vs. the fast protocol for the EZ2 RNA FFPE Kit?
FAQ ID - 3941
When should I use Phoenix Hot Start Taq GC reaction Buffer?
FAQ ID - 3903
When should I use 5X VeraSeq GC Buffer?
FAQ ID - 3910
When should I use 5X VeraSeq GC Buffer?
FAQ ID - 3923
When should I choose EasyXpress Disulfide Insect Kits?
FAQ ID -2971
When should carrier be used with the QIAamp DNA Mini or the DNeasy Blood & Tissue Kit?
FAQ ID -100
When should an intensity calibration be re-performed on the QIAxcel System?
FAQ ID -1825
When searching for a QuantiTect Primer Assay for my gene of interest, why do I only find the assay for human but not for rat?
FAQ ID -1139
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