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3330 - Is there any contamination from E. coli genomic DNA in the polymerase provided with the Repli-G Single Cell Kit?
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3329 - When using REPLI-g, if the starting material is a single sorted cell, could mitochondrial DNA (mtDNA) be amplified?
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3331 - Should the REPLI-g amplified mtDNA be cleaned up before sequencing?
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3332 - Is there a method that can distinguish between non-amplified DNA and REPLI-g amplified DNA?
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3333 - When using REPLI-g, what is the purpose of the DTT added to the denaturation buffer in the cells/blood protocol?
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What sample types can be tested on the arrays/assays?
FAQ ID — 3399
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What are the minimum sample requirements for Microbial DNA qPCR kits?
FAQ ID — 3393
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Can I use the Microbial DNA-Free Water and Microbial qPCR Mastermix if they have been opened more than 3 times?
FAQ ID — 3398
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3353 - What is the composition of the elution buffer in the Ni-NTA Fast Start Kit?
FAQ ID - 3353
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3352 - What are the size ranges of Ni-NTA particles?
FAQ ID - 3352
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3354 - What type and amount of resin is packed into the Ni-NTA Spin columns from the Ni-NTA Spin Kit?
FAQ ID - 3354
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3341 - How are the EpiTect DNA controls produced and tested?
FAQ - 3341
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3351 - What is the upper limit for protein size that can bind to Ni-NTA agarose resin?
FAQ ID - 3351
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3344 - What is the difference between the EpiTect Plus DNA Bisulfite kit compared to the classic kit?
FAQ - 3344
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3345 - What is the difference between the high and low concentration EpiTect Bisulfite Conversion protocol?
FAQ - 3345
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3311 - Can QuickLyse miniprep kits be used with QIAVac 24 Plus?
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3313 - What is the endotoxin level for the QIAGEN Plasmid Plus kits?
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3312 - For DirectPrep 96 miniprep, is it possible to use a half plate and save the other half for later prep?
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3314-What is the concentration of RNase A sold separately?
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3316-What is the concentration of the reconstituted QuantiFast Probe Assay?
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3325 - What are the catalog numbers for the packing materials for the QIAcube?
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3324 - Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube?
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3335 - Can I use the DNA directly from enzymatic reaction for Epitect Bisulfite treatment with the Epitect Bisulfite kit (cat. no. 59104)?
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3336 - Can I use the linearized DNA for Epitect Bisulfite treatment with the Epitect Bisulfite kit?
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3339 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) negatively impact subsequent NGS sequencing?
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3337 - Which method is used for QIAGEN sequencing service?
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3338 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) have 3'-hydroxyl and 5'-phosphate groups?
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3343 - Why is it difficult to quantify DNA using UV/Vis spectroscopy and fluorescence methods following EpiTect Bisulfite Conversion? How best do you quantify it?
FAQ - 3343
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3342 - How is the unmethylated unconverted EpiTect DNA control to be used?
FAQ - 3342
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3315-What is the concentration of the reconstituted QuantiTect Primer Assay (200)?
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3322 - What is the concentration of the reconstituted miScript Primer Assay (100) and miScript Precursor Assay (100) tubes?
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3317 - Do you have a protocol for isolation of genomic DNA from cell cultures using the QIAamp cador Pathogen Mini Kit?
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3318 - What guidelines should I follow in designing primers for use with the EpiTect MSP Kit?
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3320 - What is the sensitivity of the Type-it HRM PCR Kit?
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3321 - What is the purpose of the C. elegans miR-39 spike-in control in the miRNeasy Serum/Plasma kit?
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3318 - What is the preferred amplicon size for performing PCR on DNA amplified with the EpiTect Whole Bisulfitome Kit?
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3305 - What is the minimal amount of starting material required for using the SBTexcellerator HLA Kits?
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3306 - What is the optimal sample DNA concentration for the Investigator ESSplex SE Plus Kit?
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3304 - What is the maximum DNA input volume that can be used in the Investigator ESSplex SE Plus Kit?
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3310 - Which settings for the filter set Any5Dye must be adjusted before running the spectral calibration for Investigator ESSplex SE Plus Kit?
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3308 - Where can I find the template files for the Investigator ESSplex SE Plus Kit?
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3309 - Which filter set should be used with Investigator ESSplex SE Plus Kit?
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What additional equipment is needed to purify RNA using the PAXgene 96 Blood RNA Kit?
FAQ ID -499
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3300 - Can isopropanol or methanol be used in place of ethanol in RNeasy kits?
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3301 - Do you have any information or guidelines regarding the choice of reference genes for real-time PCR?
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3302 - How long can I store my purified RNA?
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3210 - Can the 4-16K Centrifuge be used with a 208 V outlet?
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3211 - What is the maximum speed of the plate rotor 2 x 96 in the 4-16 or 4-16K?
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3212 - What is the minimum size of DNA fragment that can be isolated with QIAamp DNA Mini kit?
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3299-Can Buffer ER from the Endofree Plasmid kits be used instead of the Buffer ETR in QIAGEN Plasmid Plus kits?
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3213 - How much ethanol should be added to Buffer AP3/E (renamed to Buffer AW1) and Buffer AW (renamed to Buffer AW2) in the DNeasy Plant kit?
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3202 - Can GeneRead rRNA depletion kit be used to deplete rRNA from miRNA isolated using miRNeasy kit?
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3200 - What is the Maximum/Minimum amount of RNA that can be used with GeneRead rRNA depletion kit?
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3201 - What quality of RNA can be used with the GeneRead rRNA depletion kit?
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3204 - How strict is the purification cut-off with GeneRead Size Selection kit?
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3203 - Is the RNeasy MinElute cleanup kit required for the GeneRead rRNA depletion kit?
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3205 - Can GeneRead Library preparation kits be used on species other than human mouse and rat, for example, plant species and microbial species?
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3206 - Can our GeneRead Library preparation kits be used with the SoLiD Platform?
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3248 - What is the difference between sponge, cubic and lamellar phase?
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3254 - When using the Nextal Cubic Phase products how do I know in which phase the sample is? What will I see?
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3255 - When using the NexTal Cubic Phase products, should I work with diluted or undiluted screens?
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3227 - In which solution can I keep my protein with the NexTal Cubic Phase kits?
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3225 - How pure should my protein be for the NexTal CubicPhase kits?
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3217 - How long can I store my purified RNA?
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3292-How do I start using the NeXtal CubicPhase kits?
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3295-What is the difference between SBTexcellerator HLA Core, Extended and Full Kits?
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3240 - What are the main differences between the qBiomarker PCR Arrays and the RT2 Profiler PCR Arrays?
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3237 - I would like to order the QuantiFast Probe Assays without master mix. Is this possible?
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3233 - Is the yield and quality of RNA and DNA purified with the AllPrep DNA/RNA FFPE Kit comparable to yield and quality of nucleic acids purified separately with other methods.
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3265 - Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)?
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3298-Will the sequence of the QuantiFast Probe Assays be provided?
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3262 - Which thermal cyclers are validated for use with the Investigator Nonaplex ESS Kit?
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3263 - Why are samples centrifuged at 4°C after the addition of chloroform when using the RNeasy Plus Universal Mini Kit?
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3241 - What are the recommended storage conditions of the QuantiFast Probe RT-PCR Plus Kit components?
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3264 - Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA?
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3238 - What are important points regarding the genomic DNA wipeout buffer in the QuantiFast Probe RT-PCR Plus Kit?
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3245 - What is the concentration of the primers and the probe in a reaction using the QuantiFast Probe Assays?
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3239 - What are the differences between the existing QuantiFast Probe RT-PCR Kit and the new Plus Kit?
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3247 - What is the correct matrix to use with the Investigator Nonaplex ESS Kit?
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3243 - What does the Investigator Nonaplex ESS Kit contain?
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3244 -What is the average length of a QuantiFast Probe Assay?
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3231 - Is there a size limit to the protein I can crystallize with the NexTal Cubic Phase products?
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3228 - Is it also possible to isolate miRNA with the AllPrep DNA/RNA FFPE Kit?
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3232 - Is the RNeasy Plus Universal Mini Kit suited for adipose, brain, and other fatty tissues?
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3229 - Is it necessary to perform calibration steps for the use of the MAX dye in duplex RT-PCR experiments on different cyclers?
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3236 - I use cultured cells as starting material. Can I also use the RNeasy Plus Universal Kit?
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Is there also a miRNeasy Plus Universal Kit available?
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3261 - Which purification kits are recommended for the therascreen Pyro kits?
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3258 - Which filter set and settings should be used with the Investigator Nonaplex ESS Kit?
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3260 - Which purification kits are recommended for the Pyro kits?
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3259 - Which probe labels are available for the QuantiFast Probe Assays?
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3296-Why does the A peak reduction factor have to de set to 0.86 for the codon 61 assay in the therascreen NRAS Pyro Kit?
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What plug-ins are available for the therascreen Pyro kits?
FAQ ID - 3251
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3249 - What is the optimal DNA volume and concentration for the Investigator Nonaplex ESS Kit?
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What plug-ins are available for the Pyrosequencing kits?
FAQ ID - 3250
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3252 - What should be the concentration of my protein with the NexTal Cubic Phase products?
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3256 - Which crystallization solutions are compatible with lipidic cubic phase crystallization?
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3253 - What species are QuantiTect Probe Assays offered for?
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3257 - Which deparaffinization method can I use with the AllPrep DNA/RNA FFPE Kit?
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Can the insertions in Exon 20 be detected with the therascreen EGFR Pyro Kit?
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Can the insertions in Exon 20 be detected with the EGFR Pyro Kit?
FAQ ID - 3279
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3277 - Can I use the RNeasy Plus Universal Mini Kit for all types of tissue?
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Can QuantiFast Probe Assays be ordered offline via e-mail or fax?
FAQ ID - 3278
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3283-Do you have any information or guidelines regarding the choice of reference genes for real-time PCR?
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3285-How are the genes in each iPSC qBiomarker PCR Array selected?
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3284-For which applications can the RNA isolated with the RNeasy Plus Universal Kit also be used?
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Can the QuantiFast Probe Assay detect short fragmented RNA as well as longer fragments of RNA?
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Do the RNeasy Plus Universal Mini/Midi kits contain both wash buffers, RW1 and RWT?
FAQ ID - 3282
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3270 - Are the primer sets different for the Investigator Nonaplex ESS Kit and ESSplex SE where the loci are the same?
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Are the QuantiFast Probe Assays hydrolysis or FRET probes?
FAQ ID - 3271
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Are panels and bin sets available for use with GeneMapper ID and GeneMapper ID-X?
FAQ ID - 3267
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3275 - Can I use more than 10ng DNA for the PCR reaction for my therascreen Pyro Kits?
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Can I use more than 10ng DNA for the PCR reaction for the Pyro kits?
FAQ ID - 3276
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3274 - Can I do singleplex PCR with the QuantiFast Probe Assays?
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3273 - At what temperature can I perform the experiment with the NexTal Cubic Phase kits?
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3272 - Are the QuantiFast Probe Assays wet-lab validated?
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3222 - How much DNA/RNA can be isolated with the AllPrep DNA/RNA FFPE Kit?
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3218 - How many fragments are contained within the size standard BTO in the Investigator Nonaplex ESS Kit?
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3219 - How many freeze-thaw cycles can the master mix contained in the QuantiFast Probe RT-PCR Plus Kit tolerate?
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3220 - How many PCR cycles should I use for the Investigator Nonaplex ESS Kit?
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3226 - How pure should the chloroform used with the RNeasy Plus Universal Mini Kit be?
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3223 - How much sample material can I use for DNA/RNA purification with the AllPrep DNA/RNA FFPE Kit?
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3224 - How much time can be saved when using the RNeasy Mini QIAcube Kit?
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3289-How does the QuantiFast Probe RT-PCR Plus Kit eliminate genomic DNA contamination?
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3290-How do I analyze the data for the particular iPSC qBiomarker Arrays I have ordered?
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3288-How does the gDNA eliminator solution of the RNeasy Plus Universal Kit work?
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3294-How long can I store my purified DNA/RNA isolated with the AllPrep DNA/RNA FFPE kit?
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3286-How can I adjust the ROX concentration in the QuantiFast Probe RT-PCR Plus master mix?
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3291-How do I harvest the crystals from meso phase? Are special tools needed with the NexTal Cubic Phase products?
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3246 - What is the correct matrix to use with Investigator ESSplex SE Plus Kit?
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3297-Why does the PCR for HLA-DRB4 result in 2 amplicons of different size?
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3266 - Why are two different exon 2 forward sequencing primers used for the SBTexcellerator HLA-DRB1 Kit?
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How can I find out if my gene of interest is expressed in a specific tissue type or cell line?
FAQ ID - 3214
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3215 - What is the starting material for SBTexcellerator HLA Kits?
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Can I use genomic DNA from fixed samples with qBiomarker Copy Number PCR Arrays?
FAQ ID — 3416
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Can I use amplified genomic DNA with qBiomarker Copy Number PCR Arrays?
FAQ ID — 3415
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Do the samples require pretreatment before starting the manual protocol or before loading onto the QIAcube instrument?
FAQ ID - 3479
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What are the expected yields of RNA from PAXgene Blood RNA Tubes isolated with the PAXgene Blood miRNA kit?
FAQ ID - 3481
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Do you have stability data for RNA isolated with PAXgene miRNA Kit?
FAQ ID - 3480
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Can the PAXgene Blood DNA Tubes be stored at 4°C?
FAQ ID - 3493
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How should I thaw frozen PAXgene Blood DNA Tubes?
FAQ ID - 3495
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Can the PAXgene Blood DNA Tube be frozen? If yes, at what temperatures and for how long?
FAQ ID - 3491
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Can mitochondrial DNA be isolated with the PAXgene Blood DNA System?
FAQ ID - 3498
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3497 - What is the expected purity and size of DNA extracted using the PAXgene Blood DNA kit?
FAQ ID - 3497
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Does the DNA extracted using the PAXgene Blood DNA System contain RNA?
FAQ ID - 3499
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Can I multiplex the Microbial DNA qPCR Assays?
FAQ ID — 3408
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Can I measure virulence factor gene expression?
FAQ ID — 3405
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What sample types can I test on qBiomarker Copy Number PCR Arrays?
FAQ ID — 3423
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What is the sensitivity for the Microbial DNA qPCR kits?
FAQ ID — 3409
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Which probe labels are available for the Microbial DNA qPCR Assays?
FAQ ID — 3407
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Are the Microbial DNA qPCR Assays wet-lab verified?
FAQ ID — 3406
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Can I measure antibiotic resistance gene expression?
FAQ ID — 3404
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What sequences are used to design the Microbial DNA qPCR Assays?
FAQ ID — 3403
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Are the assays species-specific?
FAQ ID — 3400
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Why is the copy number call lower than expected?
FAQ ID — 3413
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Will qBiomarker Copy Number Assays work with heterogeneous samples like mixtures of tumor and normal tissue?
FAQ ID — 3419
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Why do qBiomarker Copy Number PCR Arrays have assays in quadruplicate?
FAQ ID — 3414
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How should I analyze the data generated from a qBiomarker Copy Number PCR Array experiment?
FAQ ID — 3421
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What could have gone wrong if the CT values are unusually high for all wells in a sample?
FAQ ID — 3412
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What qPCR mastermix should I use with the qBiomarker Copy Number PCR Arrays and Assays
FAQ ID — 3422
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How much DNA should I use on a qBiomarker Copy Number PCR Array?
FAQ ID — 3417
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How much DNA should I use in a qBiomarker Copy Number PCR Assay?
FAQ ID — 3418
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How do you choose which assays to include on the arrays?
FAQ ID — 3420
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What testing should be performed in order to assess the quality of a DNA sample?
FAQ ID — 3424
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How many samples can be processed with the PAXgene Blood miRNA Kit?
FAQ ID - 3477
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Why have the hazard symbols accompanying products changed?
FAQ ID - 3430
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Why is a blood collection set required to be used with the PAXgene Blood RNA tube?
FAQ ID - 3459
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As part of my RT procedure I routinely heat an aliquot of the eluate prior to the RT reaction. Do I still need to heat the whole eluate after the RNA preparation?
FAQ ID - 3468
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Is there any chance of the PAXgene Blood RNA tube reagent going back into the patient's arm?
FAQ ID - 3460
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I need to buy a new computer for my PyroMark Q96. Can I reinstall the PyroMark Q96 software on my new computer or do I need to purchase a new license?
FAQ ID - 3472
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Can I reinstall the PyroMark Q24 software on my new computer or following an operating system upgrade, or do I need to purchase a new license?
FAQ ID - 3473
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Can the PAXgene Blood DNA Tubes be stored at higher temperatures?
FAQ ID - 3494
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What are the expected yields of RNA from PAXgene Blood RNA Tubes using the QIAsymphony PAXgene Blood RNA Kit?
FAQ ID - 3475
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What additional equipment is needed to purify RNA using the PAXgene 96 Blood RNA Kit?
FAQ ID - 3474
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Is it possible to isolate genomic DNA or viral nucleic acids with the QIAsymphony PAXgene Blood RNA protocol?
FAQ ID - 3476
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2. Are there any special considerations for the collection of blood using PAXgene Blood DNA Tubes CE (e.g., use of a blood collection set or discard tube, positioning of the tube, issues with short draws, etc.)?
FAQ ID - 3485
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Are the PAXgene Blood DNA Tubes sterile?
FAQ ID - 3487
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What is the additive in PAXgene Blood DNA Tubes
FAQ ID - 3486
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What is the maximum number of samples for the QIAcube variant of the PAXgene Blood miRNA protocol for a single run?
FAQ ID - 3482
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Do I have to use a blood collection set with the PAXgene Blood DNA Tube?
FAQ ID - 3484
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Is it possible to isolate genomic DNA or viral nucleic acids with PAXgene Blood miRNA protocol?
FAQ ID - 3483
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How should blood samples drawn into PAXgene Blood DNA Tubes be shipped?
FAQ ID - 3489
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How long can the PAXgene Blood DNA Tube be kept at room temperature? What is room temperature?
FAQ ID - 3492
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Which sample types can be processed using the PAXgene Blood miRNA protocols?
FAQ ID - 3478
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What is the shelf life of the PAXgene Blood DNA Tube prior to blood collection?
FAQ ID - 3488
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How should blood samples drawn into the PAXgene Blood DNA Tubes be stored?
FAQ ID - 3490
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Can the miScript II RT Kit be used to quantify piRNAs?
FAQ ID - 3432
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Can the miScript Plant RT Kit be used to quantify piRNA expression?
FAQ ID - 3434
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Can the miScript Plant RT Kit be used to quantify animal miRNAs?
FAQ ID - 3433
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Does the miScript Plant RT Kit contain controls?
FAQ ID - 3435
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What is the composition of the 5x miScript Plant RT Buffer?
FAQ ID - 3437
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Are any of the miScript II RT Kit components interchangeable with the miScript Plant RT Kit components?
FAQ ID - 3436
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What should I use to isolate total RNA from plant tissues and what is the maximum recommended amount of tissue input?
FAQ ID - 3438
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Are miScript Primer Assays available for plant mature miRNAs?
FAQ ID - 3439
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