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Do I have to amplify the libraries generated with REPLI-g Single Cell RNA Library Kit to obtain full-length adapter sequences?
FAQ ID - 3553
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What is the enzyme used in the whole transcriptome amplification step?
FAQ ID - 3554
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Which cell collection methods are compatible with REPLI-g Single Cell RNA Library Kit?
FAQ ID - 3555
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Should cells be washed before collection?
FAQ ID - 3556
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What is the sample input volume for REPLI-g Single Cell RNA Library Kit?
FAQ ID - 3557
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Are there special requirements for flow sorting?
FAQ ID - 3558
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What kind of starting material can be used with the REPLI-g Single Cell RNA Library Kit?
FAQ ID - 3548
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What is the maximum/minimum amount of DNA that can be used with REPLI-g Single Cell RNA Library Kit?
FAQ ID - 3549
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What is the expected purity of DNA extracted from blood samples collected in PAXgene Blood DNA Tubes IVD?
FAQ ID - 3511
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Is the quality and size of DNA extracted with the QIAamp DNA Mini and QIAamp Blood Mini kit good enough to generate DNA-libraries for next generation sequencing (NGS)?
FAQ ID - 3518
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How much protein does one vial of Protease in the QIAamp spin kits contain? What is the concentration of the resuspended Protease?
FAQ ID - 3520
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What is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?
FAQ ID - 3514
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Where can I find additional information for PreAnalytiX PAXgene products?
FAQ ID - 3515
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How can samples be shipped and stored prior to extraction on the Autopure LS?
FAQ ID - 3522
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How does the liquid level detection work and what is the minimal detectable volume for the conductive tips of the BioRobot Universal?
FAQ ID - 3524
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How can samples be shipped and stored prior to extraction on the Autopure LS?
FAQ ID - 3528
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What is the pipetting throughput of the BioRobot Universal?
FAQ ID - 3527
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How do I obtain the concentration of my gDNA from the data analysis using the GeneRead DNA QuantiMIZE?
FAQ ID - 3530
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What is the pipetting throughput of the BioRobot Universal?
FAQ ID - 3525
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What specification do you have for the high-speed shaker system of the BioRobot Universal?
FAQ ID - 3526
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Buffer SB1 in the GeneRead Size Selection Kit turned yellowish from a new kit. Can I still use it?
FAQ ID - 3562
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Is it possible to use the REPLI-g WTA Single Cell kit to amplify miRNA?
FAQ ID - 3563
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What is the sequence of the index in the GeneRead DNA Adapter I Set 1plex (catalog number 180912)?
FAQ ID - 3561
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What is the accuracy of the high-speed dispensing system of the BioRobot Universal?
FAQ ID - 3523
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Can I use the QuantiMIZE system for determination of gDNA quality for non-multiplex PCR enrichment methods (ex. Hybridization) (cat. No. NGQA-002, NGQC-100Y)?
FAQ ID - 3531
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There is no barcode on the QIAsymphony cooling adapter, and the instrument asks for one. What do you suggest I do?
FAQ ID - 3532
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Can I add bleach to the sample preparation waste?
FAQ ID - 3505
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What is the recommended range of cells for the miScript Single Cell qPCR Kit?
FAQ ID - 3565
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Can purified RNA be used with the miScript Single Cell qPCR Kit?
FAQ ID - 3566
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Can the miScript Single Cell qPCR Kit be used to quantify piRNA expression?
FAQ ID - 3567
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Can the miScript Single Cell qPCR Kit be used with plant cells?
FAQ ID - 3568
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Are any of the miScript Single Cell qPCR Kit components interchangeable with miScript II RT Kit, miScript Plant RT Kit, miScript PreAMP PCR Kit, or miScript Microfluidics PreAMP Kit components?
FAQ ID - 3569
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Should miScript PreAMP Primer Mixes be used in conjunction with the miScript Single Cell qPCR Kit?
FAQ ID - 3570
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Is the cDNA cleanup step in the miScript Single Cell qPCR Kit workflow absolutely required?
FAQ ID - 3571
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I have accidentally pipetted beads when removing the eluate after cDNA cleanup. What should I do?
FAQ ID - 3572
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Does the miScript Single Cell qPCR Kit contain controls?
FAQ ID - 3574
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When performing real-time PCR quality control associated with the miScript Single Cell qPCR Kit, is it necessary to perform control experiments for all individual samples/cells?
FAQ ID - 3576
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I do not see any miRNA expression for my miRNAs of interest after performing single cell miRNA expression analysis with the miScript Single Cell qPCR Kit and miScript miRNA PCR Arrays or miScript Primer Assays. What could be the cause?
FAQ ID - 3577
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What is the amplicon size of PCR products generated with the miScript Single Cell qPCR System?
FAQ ID - 3578
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What is the dissociation curve temperature of PCR products generated with the miScript Single Cell qPCR System?
FAQ ID - 3579
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When performing real-time PCR quality control associated with the miScript Single Cell qPCR Kit, is it necessary to perform control experiments for all individual samples/cells?
FAQ ID - 3575
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Do you recommend normalizing single cell miRNA expression data to a “housekeeping” gene or miRNA?
FAQ ID - 3580
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How should data be analyzed when the miScript Single Cell qPCR Kit is used with miScript miRNA PCR Arrays or miScript Primer Assays?
FAQ ID - 3581
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What single cell isolation methods are recommended for use with the miScript Single Cell qPCR Kit?
FAQ ID - 3582
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How would I provide information on the QuantiTect Primer assays in publications when the sequence is confidential?
FAQ ID - 3593
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In which tubes is clot detection possible on QIAsymphony?
FAQ ID - 3589
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How can I create a report/logfile on my QIAsymphony SP system?
FAQ ID - 3594
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Is it possible to measure the proteolytic activity of proteins on the surface of exosomes that were isolated with ExoEasy?
FAQ ID - 3599
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Which barcodes on sample tubes are accepted by the QIAsymphony SP?
FAQ ID - 3598
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Can I change the language on my QIAsymphony SP system?
FAQ ID - 3595
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Can I use blood, cells or bacteria with the QIAamp Fast DNA Tissue Kit?
FAQ ID - 3587
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Can the Rotor-Gene Q be connected to LIMS?
FAQ ID - 3596
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Can the REPLI-g Mitochondrial DNA Kit be used for species other than humans?
FAQ ID - 3584
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How should blood samples drawn into PAXgene Blood DNA Tubes (IVD) be stored?
FAQ ID - 3585
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How should frozen PAXgene Blood DNA Tubes (IVD) be thawed?
FAQ ID - 3586
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Which Microbial qPCR Mastermix should I use?
FAQ ID — 3395
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What are the storage conditions for the Microbial DNA qPCR products?
FAQ ID — 3394
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3327 - How often should the o-ring for the pipettor tip-adapter be changed on the QIAcube?
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3326 - Which QIAcube standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet?
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3328 - Can the Sample Tubes CB and the Sample Tubes RB be used interchangeably?
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3330 - Is there any contamination from E. coli genomic DNA in the polymerase provided with the Repli-G Single Cell Kit?
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3329 - When using REPLI-g, if the starting material is a single sorted cell, could mitochondrial DNA (mtDNA) be amplified?
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3331 - Should the REPLI-g amplified mtDNA be cleaned up before sequencing?
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3332 - Is there a method that can distinguish between non-amplified DNA and REPLI-g amplified DNA?
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3333 - When using REPLI-g, what is the purpose of the DTT added to the denaturation buffer in the cells/blood protocol?
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What sample types can be tested on the arrays/assays?
FAQ ID — 3399
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What are the minimum sample requirements for Microbial DNA qPCR kits?
FAQ ID — 3393
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Can I use the Microbial DNA-Free Water and Microbial qPCR Mastermix if they have been opened more than 3 times?
FAQ ID — 3398
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3353 - What is the composition of the elution buffer in the Ni-NTA Fast Start Kit?
FAQ ID - 3353
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3352 - What are the size ranges of Ni-NTA particles?
FAQ ID - 3352
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3354 - What type and amount of resin is packed into the Ni-NTA Spin columns from the Ni-NTA Spin Kit?
FAQ ID - 3354
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3341 - How are the EpiTect DNA controls produced and tested?
FAQ - 3341
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3351 - What is the upper limit for protein size that can bind to Ni-NTA agarose resin?
FAQ ID - 3351
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3344 - What is the difference between the EpiTect Plus DNA Bisulfite kit compared to the classic kit?
FAQ - 3344
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3345 - What is the difference between the high and low concentration EpiTect Bisulfite Conversion protocol?
FAQ - 3345
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3311 - Can QuickLyse miniprep kits be used with QIAVac 24 Plus?
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3313 - What is the endotoxin level for the QIAGEN Plasmid Plus kits?
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3312 - For DirectPrep 96 miniprep, is it possible to use a half plate and save the other half for later prep?
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3314-What is the concentration of RNase A sold separately?
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3316-What is the concentration of the reconstituted QuantiFast Probe Assay?
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3325 - What are the catalog numbers for the packing materials for the QIAcube?
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3324 - Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube?
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3335 - Can I use the DNA directly from enzymatic reaction for Epitect Bisulfite treatment with the Epitect Bisulfite kit (cat. no. 59104)?
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3336 - Can I use the linearized DNA for Epitect Bisulfite treatment with the Epitect Bisulfite kit?
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3339 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) negatively impact subsequent NGS sequencing?
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3337 - Which method is used for QIAGEN sequencing service?
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3338 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) have 3'-hydroxyl and 5'-phosphate groups?
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3343 - Why is it difficult to quantify DNA using UV/Vis spectroscopy and fluorescence methods following EpiTect Bisulfite Conversion? How best do you quantify it?
FAQ - 3343
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3342 - How is the unmethylated unconverted EpiTect DNA control to be used?
FAQ - 3342
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3315-What is the concentration of the reconstituted QuantiTect Primer Assay (200)?
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3322 - What is the concentration of the reconstituted miScript Primer Assay (100) and miScript Precursor Assay (100) tubes?
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3317 - Do you have a protocol for isolation of genomic DNA from cell cultures using the QIAamp cador Pathogen Mini Kit?
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3318 - What guidelines should I follow in designing primers for use with the EpiTect MSP Kit?
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3320 - What is the sensitivity of the Type-it HRM PCR Kit?
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3321 - What is the purpose of the C. elegans miR-39 spike-in control in the miRNeasy Serum/Plasma kit?
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3318 - What is the preferred amplicon size for performing PCR on DNA amplified with the EpiTect Whole Bisulfitome Kit?
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3305 - What is the minimal amount of starting material required for using the SBTexcellerator HLA Kits?
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3306 - What is the optimal sample DNA concentration for the Investigator ESSplex SE Plus Kit?
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3304 - What is the maximum DNA input volume that can be used in the Investigator ESSplex SE Plus Kit?
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3310 - Which settings for the filter set Any5Dye must be adjusted before running the spectral calibration for Investigator ESSplex SE Plus Kit?
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3308 - Where can I find the template files for the Investigator ESSplex SE Plus Kit?
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3309 - Which filter set should be used with Investigator ESSplex SE Plus Kit?
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What additional equipment is needed to purify RNA using the PAXgene 96 Blood RNA Kit?
FAQ ID -499
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3300 - Can isopropanol or methanol be used in place of ethanol in RNeasy kits?
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3301 - Do you have any information or guidelines regarding the choice of reference genes for real-time PCR?
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3302 - How long can I store my purified RNA?
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3210 - Can the 4-16K Centrifuge be used with a 208 V outlet?
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3211 - What is the maximum speed of the plate rotor 2 x 96 in the 4-16 or 4-16K?
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3212 - What is the minimum size of DNA fragment that can be isolated with QIAamp DNA Mini kit?
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3299-Can Buffer ER from the Endofree Plasmid kits be used instead of the Buffer ETR in QIAGEN Plasmid Plus kits?
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3213 - How much ethanol should be added to Buffer AP3/E (renamed to Buffer AW1) and Buffer AW (renamed to Buffer AW2) in the DNeasy Plant kit?
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3202 - Can GeneRead rRNA depletion kit be used to deplete rRNA from miRNA isolated using miRNeasy kit?
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3200 - What is the Maximum/Minimum amount of RNA that can be used with GeneRead rRNA depletion kit?
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3201 - What quality of RNA can be used with the GeneRead rRNA depletion kit?
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3204 - How strict is the purification cut-off with GeneRead Size Selection kit?
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3203 - Is the RNeasy MinElute cleanup kit required for the GeneRead rRNA depletion kit?
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3205 - Can GeneRead Library preparation kits be used on species other than human mouse and rat, for example, plant species and microbial species?
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3206 - Can our GeneRead Library preparation kits be used with the SoLiD Platform?
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3248 - What is the difference between sponge, cubic and lamellar phase?
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3254 - When using the Nextal Cubic Phase products how do I know in which phase the sample is? What will I see?
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3255 - When using the NexTal Cubic Phase products, should I work with diluted or undiluted screens?
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3227 - In which solution can I keep my protein with the NexTal Cubic Phase kits?
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3225 - How pure should my protein be for the NexTal CubicPhase kits?
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3217 - How long can I store my purified RNA?
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3292-How do I start using the NeXtal CubicPhase kits?
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3295-What is the difference between SBTexcellerator HLA Core, Extended and Full Kits?
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3240 - What are the main differences between the qBiomarker PCR Arrays and the RT2 Profiler PCR Arrays?
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3237 - I would like to order the QuantiFast Probe Assays without master mix. Is this possible?
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3233 - Is the yield and quality of RNA and DNA purified with the AllPrep DNA/RNA FFPE Kit comparable to yield and quality of nucleic acids purified separately with other methods.
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3265 - Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)?
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3298-Will the sequence of the QuantiFast Probe Assays be provided?
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3262 - Which thermal cyclers are validated for use with the Investigator Nonaplex ESS Kit?
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3263 - Why are samples centrifuged at 4°C after the addition of chloroform when using the RNeasy Plus Universal Mini Kit?
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3241 - What are the recommended storage conditions of the QuantiFast Probe RT-PCR Plus Kit components?
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