| Cell Line Species/Tissue: |
Rat
/
embryo |
| Transfection Reagent: |
SuperFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM + 10% FBS |
Percent Serum (%):
|
|
Reporter System:
|
b-gal |
Plasmid Purification Method:
|
2x CsCl |
Plate Format:
|
12-well plate |
Number of Cells:
|
300,000 |
Percent Confluence(%):
|
60 |
Amount of Nucleic Acid (µg):
|
1.25 |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
6.4 |
Complex Incubation on Cells (hrs):
|
2 |
Analysis Performed Post-Transfection (hrs):
|
12 |
Transfection Efficiency (%):
|
35 |
| |
| Any modifications to the protocol?:
|
| adding more medium with the DNA-superfect mix, to reduce toxicity. |
| Notes: |
| |
| References: |
| |