| Cell Line Species/Tissue: |
Monkey
/
Kidney |
| Transfection Reagent: |
PolyFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
|
Percent Serum (%):
|
10% |
Reporter System:
|
|
Plasmid Purification Method:
|
CsCl |
Plate Format:
|
100mm dish |
Number of Cells:
|
3x10e6 |
Percent Confluence(%):
|
40% |
Amount of Nucleic Acid (µg):
|
4.23µg |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
25µl |
Complex Incubation on Cells (hrs):
|
24h |
Analysis Performed Post-Transfection (hrs):
|
24h |
Transfection Efficiency (%):
|
40% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Plasmid circular, 5742bp |
| References: |
| |