Transfection Cell Database - Detailed View


Cell Line: HeLa S3

Cell Line Species/Tissue: Human / Cervix; epithelial; adenocarcinoma 
Transfection Reagent: Effectene 
Nucleic Acid:
DNA 
Growth Medium:
DMEM 
Percent Serum (%):
10 % 
Reporter System:
GFP, pTracer-SV40 (Invitrogen) 
Plasmid Purification Method:
EndoFree Plasmid Kit 
Plate Format:
96-well plate 
Number of Cells:
 
Percent Confluence(%):
Amount of Nucleic Acid (µg):
0.1 µg 
Amount of Enhancer (µl):
0.8 µl 
Amount of Reagent (µl):
2-4 µl 
Complex Incubation on Cells (hrs):
6 h 
Analysis Performed Post-Transfection (hrs):
48 h 
Transfection Efficiency (%):
40-60% 
 
Any modifications to the protocol?:
 
Notes:
 
References: