STAR Q Punch AS and STAR Q Swab AS Instruments - Quality Sensor: Advanced STR analysis with an innovative and unique internal performance control

STAR Q AS Instruments Brochure 12/2016 9 Quality Sensor: Advanced STR analysis with an innovative and unique internal performance control Better quality control checks for your STR analysis The Investigator 24plex GO! Kit comes with an integrated quality control feature, the unique Quality Sensor, which allows the generation of additional, valuable data for performance checks. It is able to confirm a successful PCR amplification and to distinguish between the absence of DNA due to improper sampling and a failed PCR amplification, as well as differentiating between degradation and inhibition. This information can be used to choose the most appropriate rework strategy and streamline the overall workflow for direct amplification with higher first success rates. With Quality Sensor you get information about: • Successful PCR amplification • Failed PCR amplification • Absence of DNA • Inhibited DNA • Degraded DNA For better quality control in your STR analysis, see www.qiagen.com/qualitysensor. Figure 8. DNA profile generated from a buccal swab. The sample shows “ski slope” morphology, characteristic of either degradation or inhibition. Because the rework strategy for these two issues is different it is important to understand which has occurred. Since the Quality Sensor peaks (bottom purple channel, far left and far right) have been successfully amplified, this demonstrates the absence of PCR inhibitors and therefore identifies DNA degradation as the problem. The lab should advise those collecting/ storing/transporting this sample of appropriate conditions to prevent this and could try adding higher amounts of DNA to the PCR for this particular sample to achieve a full profile.

Pages Overview