Plasmid Selection Guide - HiSpeed® Plasmid Kits

HiSpeed® Plasmid Kits For ultrafast purification of up to 10 mg transfection-grade plasmid or cosmid DNA Format Gravity-flow anion-exchange column, QIAfilter cartridge for lysate clearing and QIAprecipitator for isopropanol precipitation Applications PCR/Sequencing In vitro transcription/translation Cloning BAC/PAC prep Transfection of robust cells most cells primary/sensitive cells Equipment required None for Midi and Maxi QIAvac HiSpeed LS Vacuum manifold for Mega and Giga Specifications Available kit scale Culture volume* DNA yield Processing time Midi 50 ml <200 μg 45 min Maxi 150 ml <750 μg 60 min Mega† 500 ml 2.5 mg 50 min Giga† 2.5 l 10 mg 50 min * Culture volumes when using a high-copy vector. † HiSpeed Mega and Giga contain an endotoxin removal buffer.

Pages Overview