| Cell Line Species/Tissue: |
Rat
/
Hepatoma |
| Transfection Reagent: |
SuperFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
|
Percent Serum (%):
|
10% |
Reporter System:
|
Luciferase |
Plasmid Purification Method:
|
Biorad Midi |
Plate Format:
|
48-well plate |
Number of Cells:
|
|
Percent Confluence(%):
|
90% |
Amount of Nucleic Acid (µg):
|
0.7µg |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
3µl |
Complex Incubation on Cells (hrs):
|
16h |
Analysis Performed Post-Transfection (hrs):
|
48h |
Transfection Efficiency (%):
|
20-30% |
| |
| Any modifications to the protocol?:
|
| Mixed DNA/SuperFect with cells in suspension and then plated them |
| Notes: |
| |
| References: |
| |