| Cell Line Species/Tissue: |
human
/
kidney epithelial |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM 10% FBS |
Percent Serum (%):
|
10 % |
Reporter System:
|
GFP |
Plasmid Purification Method:
|
|
Plate Format:
|
60-mm dish |
Number of Cells:
|
1500000 |
Percent Confluence(%):
|
70 % |
Amount of Nucleic Acid (µg):
|
1 µg |
Amount of Enhancer (µl):
|
8 µl |
Amount of Reagent (µl):
|
10 µl |
Complex Incubation on Cells (hrs):
|
24 h |
Analysis Performed Post-Transfection (hrs):
|
24 h |
Transfection Efficiency (%):
|
70 % |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Customer compared Effectene to Lipofectamine in HEK 293T cells.
Effectene gave 70% Transfection efficiency.
Lipofecatmine gave 60% Transfection efficiency. |
| References: |
| |