| Cell Line Species/Tissue: |
Monkey
/
Kidney, SV-40 transformed |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM |
Percent Serum (%):
|
10 % |
Reporter System:
|
GFP, pTracer-SV40 (Invitrogen) |
Plasmid Purification Method:
|
EndoFree Plasmid Kit |
Plate Format:
|
96-well plate |
Number of Cells:
|
|
Percent Confluence(%):
|
? |
Amount of Nucleic Acid (µg):
|
0.1 µg |
Amount of Enhancer (µl):
|
0.8 µl |
Amount of Reagent (µl):
|
3-4 µl |
Complex Incubation on Cells (hrs):
|
6 h |
Analysis Performed Post-Transfection (hrs):
|
48 h |
Transfection Efficiency (%):
|
40-50% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| |