Transfection Cell Database - Detailed View


Cell Line: HaCat

Cell Line Species/Tissue: human / keratinocyte 
Transfection Reagent: SuperFect 
Nucleic Acid:
DNA 
Growth Medium:
DMEM 
Percent Serum (%):
10 
Reporter System:
gene of interest 
Plasmid Purification Method:
EndoFree Plasmid Kit 
Plate Format:
6-well plate 
Number of Cells:
 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
10 
Complex Incubation on Cells (hrs):
Analysis Performed Post-Transfection (hrs):
24 
Transfection Efficiency (%):
10% 
 
Any modifications to the protocol?:
 
Notes:
Analyzed transfection efficiency via FITC-labelled antibodies.  
References: