Dr. Kubista obtained his Ph.D. in chemistry at Chalmers University of Technology in Göteborg and did postdocs at La Trobe University, Melbourne, Australia, and Yale University, New Haven, USA. He invented the light-up probes for nucleic acid detection in homogeneous solution, which led to the foundation of LightUp Technologies AB as Europe´s first company focusing on quantitative real-time PCR (qPCR) based diagnostics. In 2001 Kubista set up the TATAA Biocenter as a center of excellence in qPCR and gene expression analysis. In 2014, Kubista introduced non-invasive prenatal testing (NIPT) in Sweden and started Life Genomics AB. In 2020 Kubista co-founded SimSen Diagnostics for liquid biopsy analyses. Kubista co-authored the MIQE guidelines for qPCR analysis, which is the most cited paper in molecular diagnostics, and he is a member of the CEN/ISO working group developing guidelines for molecular diagnostics. Most recently, Kubista’s team invented Two-Tailed PCR, arguably one of the most sensitive and specific methods for analyzing short nucleic acids and most suited for dPCR. Since 2007 Kubista has headed the Laboratory of Gene Expression at the Institute of Biotechnology, BIOCEV, Czech Academy of Sciences and is among the world’s 2 % most cited researchers. During the SARS-Cov-2 pandemic, Kubista’s team was one of the first in Europe to start testing on February 3, 2020.