FAQ 

 

You are not authorized to download the resource

What elution formats can be used for the QIAsymphony DSP Virus/Pathogen protocols?
FAQ ID -2937
When should I choose EasyXpress Disulfide Insect Kits?
FAQ ID -2971
Which elution volumes can be used for the QIAsymphony DSP Virus/Pathogen protocols?
FAQ ID -2938
What sample volumes can be used with the tissue HC and LC protocols on the QIAsymphony SP?
FAQ ID -2992
What types of templates can be used for expression of antibody fragments?
FAQ ID -2964
Why do I have to use an ACS (Assay Control Set)?
FAQ ID -2933
Which kit size should be used for each QIAsymphony DSP Virus/Pathogen protocol?
FAQ ID -2930
Will mitochondrial DNA also be isolated using the DNA tissue protocols and QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP?
FAQ ID -2991
Which sample types can be processed using the QIAsymphony PAXgene Blood RNA Protocol?
FAQ ID -2981
What pathways and diseases are currently covered for the qBiomarker Somatic Mutation PCR Arrays?
FAQ ID -2922
Why does the template for EasyXpress Disulfide Insect Kits need to have a signal sequence (such as the mellitin signal sequence as provided by the EasyXpress Linear Template Fab Kit primers)?
FAQ ID -2967
What sample input formats can be used for the QIAsymphony DSP Virus/Pathogen protocols?
FAQ ID -2936
What is the starting volume for the QIAsymphony mericon Bacteria Kit?
FAQ ID -2956
What is the Limit of Detection (LOD) for an ELISArray Kit?
FAQ ID -2907
What is the sensitivity of the assays contained on the qBiomarker Somatic Mutation PCR Arrays?
FAQ ID -2910
What is the linear range of the Multi-Analyte ELISArray Kits?
FAQ ID -2909
What are the expected DNA yields from tissue processed on the QIAsymphony SP?
FAQ ID -2990
What is the maximum number of GOIs that I can detect with the Type-it CNV Probe Master Mix in the same qPCR reaction?
FAQ ID -2998
What are the recommended lysis times for tissue samples on the QIAsymphony SP?
FAQ ID -2993
What are the technological principles behind somatic mutation PCR arrays and assays?
FAQ ID -2924
What PCR machine do I need to use to run such a qBiomarker Somatic Mutation PCR Arrays?
FAQ ID -2917
What method(s) do you recommend as an alternative way to validate the mutations identified on the somatic mutation PCR arrays?
FAQ ID -2920
What sample types can I test on the qBiomarker Somatic Mutation PCR Arrays?
FAQ ID -2919
What sample volumes can be processed using the QIAsymphony DSP Virus/Pathogen protocols?
FAQ ID -2929
What sample types should be processed with the low content (LC) and high content (HC) protocols for DNA extraction on the QIAsymphony SP?
FAQ ID -2989
What samples can the QIAsymphony mericon Bacteria Kit be used for?
FAQ ID -2958
What sample types can be processed using the QIAsymphony DSP Virus/Pathogen protocols?
FAQ ID -2928
Do you have stability data for RNA isolated with the QIAsymphony PAXgene Blood RNA Kit?
FAQ ID -2983
What sample types can be processed using the tissue protocols and the QIAsymphony DSP DNA Mini Kit?
FAQ ID -2925
Do you need to pre-treat food samples before DNA isolation using the QIAsymphony mericon Bacteria Kit on the QIAsymphony SP?
FAQ ID -2957
Do the samples require pretreatment before loading onto the instrument with the QIAsymphony PAXgene Blood RNA Protocol?
FAQ ID -2982
How do you decide on the qBiomarker Somatic Mutation PCR Arrays to include on the pathway arrays and disease arrays?
FAQ ID -2913
Do the SureSilencing shRNA Plasmids contain inducible promoters?
FAQ ID -2904
How many freeze-thaw cycles can the Internal Control supplied with Certal Residual DNA Detection Kits tolerate?
FAQ ID -2975
How many mutations can I profile per sample on the qBiomarker Somatic Mutation PCR Array?
FAQ ID -2911
How can I estimate the size of my protein based on the length of the respective cDNA?
FAQ ID -2952
How do I use the qBiomarker Somatic Mutation PCR Arrays?
FAQ ID -2923
Can I use the Type-it CNV kits and Reference Assays for CNV studis in mice?
FAQ ID -2997
Can the Certal CHO Positive Control DNA be used to create a qPCR standard for relative quantification?
FAQ ID -2979
Can I use lysates or tissue homogenates with the ELISArray kits?
FAQ ID -2905
Can the EasyXpress Linear Template Fab Kit be used to express entire IgGs?
FAQ ID -2966
Can I use TaqMan® Copy Number Assays from LIFE Technologies in combination with the Type-it CNV Probe kit?
FAQ ID -2999
Can I use the SureSilencing shRNA Plasmids with primary cells or macrophages or for injection into live animals?
FAQ ID -2902
Do I have to use an internal control with each QIAsymphony DSP Virus/Pathogen Kit protocol run?
FAQ ID -2932
Do samples require pretreatment before they are loaded onto the QIAsymphony instrument?
FAQ ID -2931
Can the QIAsymphony DSP Virus/Pathogen system be used for diagnostic or prognostic procedures?
FAQ ID -2939
Do the ELISArray Kits work on species other than human, mouse or rat?
FAQ ID -2906
Does the QIAsymphony mericon Bacteria Kit contain an extraction control?
FAQ ID -2961
Is it possible to simultaneously isolate DNA and RNA from Saliva Stabilized in RNAprotect® Saliva Reagent?
FAQ ID -2996
Is it possible to use templates with signal sequences with EasyXpress Disulfide E. coli Kits?
FAQ ID -2969
Is food DNA co-purified using the QIAsymphony mericon Bacteria Kit?
FAQ ID -2959
Is it possible to introduce an affinity tag using the EasyXpress Linear Template Fab Kit?
FAQ ID -2972
Is it possible to isolate DNA from Saliva Stabilized in RNAprotect® Saliva Reagent?
FAQ ID -2995
On what cyclers have Certal Residual DNA Detection Kits been validated?
FAQ ID -2978
On which cyclers can Certal Residual Detection Kits be used?
FAQ ID -2973
Should I use the SureSilencing shRNA Plasmids with the GFP, neomycin, or hygromycin, or puromycin resistance marker?
FAQ ID -2900
Is it possible to use the native signal sequence of antibodies with EasyXpress Disulfide Insect Kits?
FAQ ID -2968
Is RNA co-purified using the QIAsymphony mericon Bacteria Kit?
FAQ ID -2960
How much DNA does a bacterial cell contain?
FAQ ID -2947
How many samples can be processed with the QIAsymphony DSP Virus/Pathogen Kits?
FAQ ID -2935
How many reactions can be performed with one QIAsymphony mericon Bacteria Kit?
FAQ ID -2955
How should the samples be stored before purification of RNA with the QIAsymphony PAXgene Blood RNA Protocol?
FAQ ID -2984
How should the samples be stored before purification with the QIAsymphony DSP Virus/Pathogen Kits?
FAQ ID -2934
If a cycler from Applied Biosystems is used in combination with the Certal Residual DNA Detection Kit, should dye calibration for MAX be performed?
FAQ ID -2977
How much sample is required for each well for an ELISArray Kit?
FAQ ID -2908
How much starting material can be used with the QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP?
FAQ ID -2927
3198 - What is the optimal DNA concentration for the Investigator DIPlex Kit?
3194 - What DNA fragment sizes are required for the Investigator DIPlex kit?
3195 - Where can I find the Population Data for the Investigator DIPlex kit?
3196 - What is the DIPsorter Freeware?
3188 - How many His residues are required for successful purification on Ni-NTA resins?
3190 - What is the smallest elution volume for RNeasy Mini Spin column?
Why does the QIAcube sometimes fail to pick up the tips or pick up wrong tips?
FAQ ID -3131
The kit says I can use up to 30mg of tissue. When I used 15mg I got a higher yield than when I used 30mg. Why?
FAQ ID -3117
I want to purify RNA from heart tissue. What is the best kit to use?
FAQ ID -3116
Why does the upper aqueous phase look pinkish when purifying RNA from fatty tissue?
FAQ ID -3118
Is it possible to partially use RNeasy 96 plates and use the plate multiple times?
FAQ ID -3124
Can I use the QIAGEN deparaffinization solution with the IDH 1/2 RGQ PCR kit?
FAQ ID -3100
Can I use stained slide materials with IDH 1/2 RGQ PCR kit?
FAQ ID -3101
What is the standard format for Flexiplate siRNAs delivery?
FAQ ID -3104
How many transfections can be performed with Flexiplate siRNAs?
FAQ ID -3105
Where to look for ordering Custom siRNAs online?
FAQ ID -3106
What is the difference between HiPerFect and HiPerFect HTS?
FAQ ID -3108
What are the miScript Controls that can be used with miScript HiSpec Buffer or miScript HiFlex Buffer?
FAQ ID -3111
What is the amplicon size of the PCR product when using the miScript primer assay?
FAQ ID -3156
What is the difference between EpiTect Bisulfite Kit and EpiTect Plus Bisulfite Kit?
FAQ ID -3167
What are the stopping points in the QIAamp protocol?
FAQ ID -3157
What are the benefits of PyroMark Q24 Advanced versus PyroMark Q24?
FAQ ID -3158
Do you have data on the DNA yield at different incubation time for the Repli-G Kits?
FAQ ID -3113
Is it possible to upgrade existing PyroMark Q24 instruments to PyroMark Q24 Advanced or do I have to purchase a new instrument?
FAQ ID -3162
Is it possible to run therascreen mutation or therascreen Pyro kits on PyroMark Q24 Advanced system?
FAQ ID -3161
Which operating system is compatible with PyroMark Q24 Advanced application software?
FAQ ID -3164
What is the adapted annealing protocol for PyroMark Q24 Advanced procedure?
FAQ ID -3166
What is the miRNA yield from the miRNeasy Serum/Plasma Kit?
FAQ ID -3171
What is the RNA size distribution recovered from the miRNeasy Serum/Plasma Kit?
FAQ ID -3172
What could be a reason for higher viscosity of PCR reaction with STR Plus chemistry?
FAQ ID -3176
Does QIAGEN provide template files for GeneMapper® 5 software?
FAQ ID -3177
Can Rotor-Gene Reports be modified?
FAQ ID -3184
What is the best way to ensure the Rotor-Disc is properly sealed when using the Heat Sealer?
FAQ ID -3179
What to do if there is a loss of communication for the Rotor-Gene Q?
FAQ ID -3180
Aside from the OTV test, what other maintenance should be done for the Rotor-Gene Q?
FAQ ID -3181
Are the miRNA Target Protector & miRNA mimic bench tested? Any guarantee?
FAQ ID - 3186
3187 - Why does the Ni-NTA resin turn reddish-brown after mixing with the lysates?
3191 - Is the Allprep DNA Mini Spin Column the same as the gDNA eliminator column? Can I interchange these columns?
3199 - Which species can be used with the GeneRead rRNA depletion kit?
3192 - Is QIAGEN Protease compatible with Buffer ATL?
What is the bacterial ribosomal RNA recommended to use in the miRNeasy Serum/Plasma Protocol?
FAQ ID -3173
3193 - Where can I get an RNase-free syringe that is recommended for reconstituting QIAGEN's RNase-free DNase (cat. no. 79254)?
Would co-transfection with the plasmid be expected to increase/decrease the transfection efficiency significantly?
FAQ ID -3136
How can I know whether there is any genomic DNA present in my purified plasmid?
FAQ ID -3134
How do I know whether my miRNA has been successfully purified and how much is expected?
FAQ ID -3135
Is it possible to use RNAprotect Cell Reagent to stabilize buffy coat?
FAQ ID -3139
Has QIAGEN ever tested Proteinase K treatment for a time period longer than overnight?
Is RNAprotect also suitable for the stabilization of miRNA?
FAQ ID -3143
Does RNAprotect affect morphology of tissues?
FAQ ID -3142
Are miScript primer assays bench-validated?
FAQ ID -3145
What is the maximum cDNA length that can be synthesized by QuantiTect Reverse Transcription Kit?
FAQ ID -3146
Why does the DNeasy mericon Food extraction kit use a QIAquick column and not DNeasy column?
FAQ ID -3148
What are the minimal and maximum fragment sizes for the QIAquick column in the mericon DNeasy kit?
FAQ ID -3147
Does RNase-free DNase I affect the isolation of dsRNA?
FAQ ID -3153
Can single-stranded DNA be removed by gDNA Eliminator columns?
FAQ ID -3152
How is total RNA purified with RNeasy kits free of genomic DNA contamination?
FAQ ID -3154
Is it possible to quantify protein content in RLT lysates?
FAQ ID -3155
What primary cell lines have been tested with HiPerFect Transfection Reagent?
FAQ ID -3137
Is it possible to use the protein resuspended in ALO buffer for downstream BCA assay and Bradford assay?
FAQ ID -3138
What are the miScript Controls that can be used with miScript HiFlex Buffer ONLY?
FAQ ID -3112
How long can I store an RLT lysate?
FAQ ID -3115
Do you have a protocol for the isolation of genomic DNA from yeast/fungi directly from Blood?
FAQ ID -3150
Is there cross talk between channels in the Rotor-Gene Q?
FAQ ID -3185
3197 -Where can I find the Population Data for the Investigator HDplex kit?
Can QIAzol aqueous phase be used with the gDNA eliminator columns in RNeasy Plus mini kit?
FAQ ID -3151
What is in the HiSpec Buffer that selectively synthesizes cDNA from mature miRNAs ONLY?
FAQ ID -3168
What is the optimal DNA concentration for the Investigator ESSplex Plus Kit?
FAQ ID -2646
Why do I see low, poor, or sub-standard amplification efficiency in my qRT-PCR assay?
FAQ ID -2695
What is the difference between adding the Internal Control (IC) template used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit to the amplification reaction versus adding the IC template at the extraction step?
FAQ ID -2600
Is the QuantiFast SYBR Green PCR Kit compatible with the ViiA7 cycler from Applied Biosystems?
FAQ ID -2652
What are the most reliable methods for preparing high-quality RNA from cell or tissue samples, for use in gene expression analysis experiments?
FAQ ID -2657
What should the cycler set-up be for a duplex reaction with the pathogen assay (FAM) and the Internal Control assay (MAX) on ABI instruments, when using the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2610
What should the cycler set-up be for a duplex reaction with the pathogen assay (FAM) and the Internal Control assay (MAX) on Mx instruments from Stratagene (Agilent), when using the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2609
What should I do if I suspect that my RNA preparation contains RNase contamination?
FAQ ID -2661
What real-time cycler should I use for my qPCR experiments?
FAQ ID -2670
Which DNA/RNA extraction kits were tested in combination with the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2606
What should the Rotor-Gene Q cycler settings be for a duplex reaction with the pathogen assay (FAM) and the Internal Control assay (MAX), when using the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2608
What testing should be performed to assess the quality of an RNA sample?
FAQ ID -2660
For the QuantiFast Pathogen RT-PCR +IC kit, does the Internal Control also control for the reverse transcription reaction?
FAQ ID -2601
Do we have data on long term stability and storage of DNA purified on BioRobot M48?
FAQ ID -2611
Following thermal cycling, reaction volume has evaporated from some of the wells of my qPCR assay plate. What should I do?
FAQ ID -2683
How can I predict the percent qPCR signal due to contaminating DNA, for a given qPCR assay, and its matching NRT control?
FAQ ID -2688
How can I ensure that reaction volume is not lost due to evaporation during thermal cycling?
FAQ ID -2679
How can I determine whether amplification occurs from mRNA-derived cDNA or from genomic DNA contamination?
FAQ ID -2687
Can the Internal Control DNA or RNA be added directly to the sample?
FAQ ID -2602
Do the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit contain ROX in the master mix?
FAQ ID -2605
Are primers available that only detect mitochondrial DNA encoded genes and not nuclear genomic DNA encoded genes?
FAQ ID -2680
Do I need to run a standard curve before the actual PCR array experiment?
FAQ ID -2664
Does the amount of Internal Control to be spiked into the lysis buffer or lysate depend on the elution volume only?
FAQ ID -2604
May I try the data analysis tool without using your PCR array kit?
FAQ ID -2698
Is the QuantiFast Multiplex PCR +R Kit compatible with the ViiA7 cycler from Applied Biosystems?
FAQ ID -2653
Is the QuantiFast Probe PCR +ROX Vial Kit compatible with the ViiA7 cycler from Applied Biosystems?
FAQ ID -2651
Is it good to pool multiple RNA replicates to detect expression changes that are consistently reproducible?
FAQ ID -2663
How important is the RNA purification process, for obtaining reliable qRT-PCR results?
FAQ ID -2655
Is a special processing protocol needed?
FAQ ID -2523
Is it possible to archive PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue blocks?
FAQ ID -2524
The disruption of my sample worked well with the TissueLyser LT, but I am not satisfied with the RNA yield. What do you suggest?
FAQ ID -2555
What are the dimensions of the TissueLyser LT?
FAQ ID -2545
What is the average RNA yield from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -2534
On which cyclers can the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit be used?
FAQ ID -2596
What are the storage conditions for the mericon Assay kits?
FAQ ID -2540
What is in the elution buffer in the PAXgene Bone Marrow RNA kit?
FAQ ID -2511
Is it possible to microdissect PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissues?
FAQ ID -2531
Is the performance of the HiPerFect HTS Reagent affected if it has accidentally been stored at°C?
-20
Is it possible to embed PAXgene Tissue fixed and stabilized samples in Optimal Cutting Temperature (OCT) medium for freezing?
FAQ ID -2525
Is the morphology after H&E staining comparable to formalin-fixed samples?
FAQ ID -2526
What RNA yields are expected with the PAXgene Bone Marrow RNA System?
FAQ ID -2506
What is the maximum tissue size that can be fixed in a PAXgene Tissue Container?
FAQ ID -2518
What is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?
FAQ ID -2538
What is the expected amplicon size when using the mericon Assay kits?
FAQ ID -2544
What is the typical amount of tissue used for disruption with the TissueLyser LT?
FAQ ID -2552
What is the longest period you recommend to leave out the dilute HiPerFect HTS at RT before use?
FAQ ID -2560
What kind of sample tubes are recommended to use with the TissueLyser LT?
FAQ ID -2553
What is the purity of nucleic acids extracted with the PAXgene Tissue kits?
FAQ ID -2533
Can the PAXgene Bone Marrow RNA System be used to isolate genomic DNA or viral RNA and DNA?
FAQ ID -2507
Can the PAXgene Bone Marrow RNA System be used with animal bone marrow or with blood samples?
FAQ ID -2501
Can the PAXgene RNA stabilization solution/bone marrow mixture from the PAXgene Bone Marrow RNA Tube be disposed of by adding bleach?
FAQ ID -2515
Can sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for other histochemical staining techniques, such as PAS?
FAQ ID -2530
Can the PAXgene Bone Marrow RNA System be used for other human body fluids than bone marrow samples or cultured cells of human origin?
FAQ ID -2502
Can the sample tubes and the TissueLyser LT Adapter be cooled with liquid nitrogen?
FAQ ID -2549
Can the TissueLyser LT Adapter be removed for cleaning?
FAQ ID -2546
Can the samples on the TissueLyser LT be protected from degradation of nucleic acids or proteins?
FAQ ID -2548
When using the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit, what should the fluorescent label of the probe be for the customer-defined assay to detect the pathogen?
FAQ ID -2594
When using HiPerFect HTS Reagent is it necessary to optimize amount of siRNA and transfection reagent?
FAQ ID -2556
What sample numbers can be processed on the TissueLyser LT?
FAQ ID -2547
What size bead should I use for tougher samples with the TissueLyser LT?
FAQ ID -2554
When performing the Internal Control assay for the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit, what channel or filter can be used to detect MAX?
FAQ ID -2597
Why is the fluorescence signal of the Internal Control on my Rotor-Gene Q very low?
FAQ ID -2574
Can specific types of white blood cells be enriched from a PAXgene Bone Marrow RNA Tube prior to the RNA isolation?
FAQ ID -2508
Can bone marrow aliquots other than 2 mL be pipetted into the PAXgene Bone Marrow RNA Tube?
FAQ ID -2503
Are the Internal Controls used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit homologous to a known target?
FAQ ID -2598
Can I use water to elute the RNA in the PAXgene Bone Marrow RNA Kit?
FAQ ID -2512
Can PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for in situ hybridization?
FAQ ID -2529
Can bone marrow from leukemia patients with elevated numbers of white blood cells be processed with the PAXgene Bone Marrow RNA System?
FAQ ID -2510
Can HiPerFect HTS be used for miRNA?
FAQ ID -2561
Can HiPerFect HTS Reagent be used for transfecting shRNAs?
FAQ ID -2559
How long does the sample disruption with the TissueLyser LT take?
FAQ ID -2550
How many times can the Internal Controls used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit be freeze-thawed?
FAQ ID -2599
How long can I store the dilutions of the Control DNA Z1 standards at 2°C?
-8
How long can primer and probes from the mericon Assay kits be kept at°C once reconstituted?
-20
How should the PAXgene Bone Marrow RNA Tubes be frozen after collection and for how long?
FAQ ID -2505
I routinely heat an aliquot of the RNA eluate prior to my RT reaction. Do I still need to heat the eluate after the PAXgene Bone Marrow RNA preparation?
FAQ ID -2513
How much time may the aspirated bone marrow sample be allowed to incubate before pipetting it into the PAXgene Bone Marrow RNA Tube?
FAQ ID -2504
Do you have FACS or Fluoro-Microscopy data using HiPerFect HTS Reagent for delivery of siRNA?
FAQ ID -2557
How is stability and performance of these mericon Assay kits guaranteed?
FAQ ID -2542
How are the supernatants resulting from the first two spin steps of the PAXgene Bone Marrow RNA kit purification procedure disposed?
FAQ ID -2516
What are typical yields of bisulfite converted DNA when using the EpiTect Plus Bisulfite Kits?
FAQ ID -2408
What batch sizes can be selected with the PAXgene Blood RNA MDx Kit?
FAQ ID -2491
What are the labels of the probe which is used in the Internal Control Assay for detection of the IC?
FAQ ID -2449
What is the detection limit for the QuantiFast Pathogen + IC kits?
FAQ ID -2453
In which solution can I keep my protein for the NexTal Cubic Phase kits?
FAQ ID -2416
What is the difference between QuantiTect Virus Kit and the new QuantiFast Pathogen +IC Kits?
FAQ ID -2448
Is there a critical step during upfront sample handling when using the PAXgene Blood RNA MDX kit?
FAQ ID -2493
Which EpiTect Bisulfite Plus kit do you recommend for blood samples, cells, and tissues?
FAQ ID -2411
What is the nature of the Internal Control in the QuantiFast Pathogen + IC kit?
FAQ ID -2450
Where can I find general information about RT-PCR and PCR arrays such as information about sample preparation, reverse transcription, quantitative PCR, real-time PCR, and PCR arrays?
FAQ ID -2446
What is the maximum number of batches for the QIAsymphony PAXgene Blood RNA protocol for a single run?
FAQ ID -2498
Why should I work with diluted or undiluted screens?
FAQ ID -2419
Why should I use the EpiTect Plus FFPE Bisulfite Kit instead of isolating FFPE DNA first and then doing the bisulfite conversion?
FAQ ID -2412
What is the difference between sponge, cubic and lamellar phase of the NeXtal CubicPhase crystallisation?
FAQ ID -2420
Where are the somatic mutations published?
FAQ ID -2429
What should be the concentration of my protein for the Nextal Cubic Phase products?
FAQ ID -2417
What kind of control samples should I use for the particular iPSC-qBiomarker Arrays I have ordered?
FAQ ID -2441
What is the range indicated by 'Std. Error' on 'Results' page?
FAQ ID -2456
Can the PAXgene Blood RNA MDx Kit be used with both the BioRobot Universal System and the BioRobot MDx?
FAQ ID -2489
Can the Internal Controls be ordered separately in the QuantiFast Pathogen + IC kit for use during purification?
FAQ ID -2451
Can the PAXgene Blood RNA MDx Kit be used for diagnostic or prognostic procedures?
FAQ ID -2488
Can the QIAsymphony PAXgene Blood RNA System be used for diagnostic or prognostic procedures?
FAQ ID -2496
Can the PAXgene Blood miRNA System be used for diagnostic or prognostic procedures?
FAQ ID -2494
At what temperature can I perform the NexTal Cubic Phase experiment and incubate the plate?
FAQ ID -2415
How are the different mutations for a single gene normalized on the qBiomarker Somatic Mutation PCR array?
FAQ ID -2428
How can I check DNA sample quality before using the Somatic Mutation PCR Arrays & Assays?
FAQ ID -2430
How much DNA starting sample is needed for analysis with a Somatic Mutations PCR Array? And Assay?
FAQ ID -2431
How do I get started using the NeXtal CubicPhase kits?
FAQ ID -2413
How pure should my protein be with the NexTal CubicPhase kits?
FAQ ID -2414
How many samples can be processed with the QIAsymphony PAXgene Blood RNA Kit?
FAQ ID -2497
How many samples can be processed with the PAXgene Blood RNA MDx Kit?
FAQ ID -2490
In the REST software, what is the range indicated by 'Std. Error' on the 'Results' page?
FAQ ID -2455
How many PAXgene Blood RNA Tubes can be processed per PAXgene 96 Blood RNA Kit well plate?
FAQ ID -2485
If less than 48/96 samples are being processed, is it possible to leave positions empty with the PAXgene Blood RNA MDx Kit?
FAQ ID -2492
How does the DNA Protection work in the EpitTect Bisulfite kits?
FAQ ID -2410
How is the hypothesis test performed in the REST software?
FAQ ID -2459
How do I determine if my gene expression results are statistically significant in the REST software?
FAQ ID -2458
How many different genes and gene mutations are on a Somatic Mutation PCR Array?
FAQ ID -2427
How long does a QuantiFast Pathogen + IC run take on the Rotor-Gene Q?
FAQ ID -2452
How do I know in which phase of my NeXtal CubicPhase cristallisation experiment I am?
FAQ ID -2421
How great is the enrichment of miRNA when using the dedicated PAXgene Blood miRNA Kit compared to the PAXgene Blood RNA Kit?
FAQ ID -2495
How do I harvest the crystals from meso phase? Are special tools needed?
FAQ ID -2422
Why are two reagents used in the PAXgene Tissue System?
FAQ ID -3022
What is the maximum size of tissue that can be fixed in a PAXgene Tissue FIX Container (50 ml)?
FAQ ID -3024
5. What is the maximum size of the tissue sample that can be fixed in a PAXgene Tissue Container?
FAQ ID -3023
What happens if a tissue sample larger than 20 x 20 x 20 mm is used for fixation with the PAXgene Tissue FIX Container (50 ml)?
FAQ ID -3025
How long is the fixation time when using PAXgene Tissue FIX Container?
FAQ ID -3026
Is it possible to fix over a weekend?
FAQ ID -3027
How long can a tissue specimen stay in the PAXgene Tissue STABILIZER?
FAQ ID -3028
What is the minimum incubation time required when using the PAXgene Tissue STABILIZER?
FAQ ID -3029
Is it possible to integrate the PAXgene Tissue STABILIZER into the processing?
FAQ ID -3037
Is it possible to archive PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3038
Is the morphology after H&E staining comparable to formalin-fixed samples?
FAQ ID -3039
Are immunohistochemistry (IHC) assays developed for formalin-fixed, paraffin-embedded (FFPE) compatible with PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3040
Is it possible to use formalin-fixed, paraffin-embedded (FFPE) kits and protocols for extraction of biomolecules from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3043
is the purity of nucleic acids isolated with the PAXgene Tissue Kits?
FAQ ID -3044
What is the integrity of RNA from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3046
What is the average RNA yield from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3045
What is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?
FAQ ID -3050
What is the PCR performance of DNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue compared to DNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?
FAQ ID -3051
How can proteins purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be analyzed?
FAQ ID -3052
What is the DNA integrity from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3047
Does the PAXgene Tissue RNA Kit co-purify small RNAs?
FAQ ID -3048
How can I extract proteins from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3049
Can PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for in situ hybridization?
FAQ ID -3042
What are the typical yields obtained from Buccal swabs on BioSprint 96?
FAQ ID -3071
Can I use a USB drive to transfer protocols to BioSprint?
FAQ ID -3072
Is it possible to manually process PAXgene Tissue treated samples without using an automated processor?
FAQ ID -3036
Are PAXgene Tissue Containers suitable for long term-storage at freezing temperatures?
FAQ ID -3030
Is it possible to process formalin-fixed and PAXgene Tissue fixed samples together in one run?
FAQ ID -3033
Is it necessary to clean a processor normally used for formalin-fixed tissue before using it with PAXgene Tissue fixed tissue?
FAQ ID -3034
Is a special processing protocol needed?
FAQ ID -3035
Can sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for other histochemical staining techniques, such as PAS?
FAQ ID -3041
Are the multicopy reference assays for Type-it CNV PCR +qC Kits and the qBiomarker assays the same?
FAQ ID -3000
How do I clean up RNA preparations containing miRNA?
FAQ ID -3002
What is the fixation method used in the PAXgene Tissue System?
FAQ ID -3019
What does PAXgene Tissue FIX contain?
FAQ ID -3020
Can I perform the IDH 1/2 RGQ PCR test with slides containing less than 40% tumor cells?
FAQ ID -3098
Can the MaXtract High Density Tube be used with QIAzol to isolate RNA?
FAQ ID -1304
What volume of bacterial culture can be processed using the QuickLyse Miniprep Kit?
FAQ ID -1357
What factors determine if denatured proteins accumulate underneath, or inside the gel of the MaXtract Low and High Density Tubes?
FAQ ID -1312
I have a Human Druggable Genome siRNA Set V2.0. Can I reorder the siRNAs from this set?
FAQ ID -1366
If both MaXtract Low Density and High Density is suitable for an application, which type is preferable to use?
FAQ ID -1310
How will service work carried out on my QIAGEN BioRobot be documented?
FAQ ID -1331
If I cannot order FlexiPlate siRNA online, how do I order?
FAQ ID -1371
What information about my gene or siRNA do I need to order FlexiPlate siRNA?
FAQ ID -1365
Is it possible to use MaXtract for an organic extraction with a mixture of phenol and BCP (1 bromine chlorpropane)?
-3
What information is provided with FlexiPlate siRNAs?
FAQ ID -1363
Is it possible to elute plasmid DNA obtained with the QuickLyse Miniprep Kit in nuclease-free water?
FAQ ID -1355
Which formats are available for FlexiPlate siRNA?
FAQ ID -1358
When isolating DNA from plant cells using CTAB, should MaXtract Low or High Density be used?
FAQ ID -1308
Which parts are exchanged during a Preventive Maintenance visit for my QIAGEN BioRobot?
FAQ ID -1334
Which controls can I choose for FlexiPlate siRNA?
FAQ ID -1368
Which density gel type is contained in the yellow or green MaXtract Tubes?
FAQ ID -1315
What is the composition of FlexiPlate siRNA buffer?
FAQ ID -1361
Which software updates are included in a Premium Cover Agreement for my QIAGEN Instrument?
FAQ ID -1336
Can MaXtract tubes be autoclaved prior to use?
FAQ ID -1305
Can I reduce the amount of starting material below 10 ng when using the REPLI-g UltraFast Mini Kit?
FAQ ID -1325
Can I reduce the reaction time to e.g., 30 minutes, when using the REPLI-g UltraFast Mini Kit?
FAQ ID -1326
Can I use any type of DNA extraction method to purify template DNA for amplification using the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1321
Can several extraction steps be performed in the same MaXtract tube?
FAQ ID -1302
Can a phenol-chloroform ratio different from 1:1 also be used with MaXtract Low and High Density?
FAQ ID -1309
Can DNA isolated with MaXtract tubes be used for downstream applications such as restriction digestion, labeling, blotting, PCR, and automated sequencing?
FAQ ID -1303
Can I amplify mitochondrial DNA directly from whole blood or cells using the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1319
Can I order Alexa Fluor labeled controls with FlexiPlate siRNA?
FAQ ID -1369
Can HP Validated siRNAs be ordered on the FlexiPlate?
FAQ ID -1367
Can frozen sample material pulverized in liquid nitrogen be directly added to MaXtract tubes?
FAQ ID -1301
Can I also screen large constructs like BACs, fosmids, and cosmids using the QIAGEN PlasmidAmp Kit?
FAQ ID -1337
How much REPLI-g amplified mitochondrial DNA should be used in a subsequent PCR reaction?
FAQ ID -1320
Do mutations in the target mitochondrial DNA prevent primer binding, resulting in no DNA amplification with the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1323
Do you have stability data for genomic DNA isolated with the BioSprint Kits?
FAQ ID -1318
Do you have stability data for genomic DNA isolated with the Flexigene DNA Kit?
FAQ ID -1317
Does the addition of sample and organic solution to the MaXtract Tubes require a specific pipetting sequence?
FAQ ID -1307
How can I upload my gene list for FlexiPlate siRNA to GeneGlobe?
FAQ ID -1364
How does a Service Support Agreement provide defined budgeting for maintaining my BioRobot?
FAQ ID -1330
How can I ensure optimal performance of my QIAGEN instrument?
FAQ ID -1329
Is it possible to purify viral RNA using FastLane Cell RT-PCR Kits?
FAQ ID -1484
Why is the activation time for HotStarTaq Plus Polymerase in the QuantiFast SYBR Green Kits different from that for QuantiFast Probe Kits?
FAQ ID -1449
Why is the RT step with the QuantiFast RT Kits much shorter compared to QuantiTect RT Kits?
FAQ ID -1451
Why is the storage time for QuantiFast PCR Kits shorter than that for QuantiTect PCR Kits?
FAQ ID -1446
Why is the reaction volume for QuantiFast PCR Kits lower than that for QuantiTect PCR Kits?
FAQ ID -1447
Why is the QuantiFast denaturation step different for PCR and RT-PCR runs in the two-step protocol for the ABI 7500 and other cyclers?
FAQ ID -1442
What is the minimum number of siRNAs per order? If I order 40 siRNAs, can I get those on 2 plates?
FAQ ID -1468
Can purification columns from other suppliers be processed on the QIAcube?
FAQ ID -1406
Can QIAcube protocol runs be interrupted, and subsequently be continued to completion?
FAQ ID -1414
Can I use more than 4 µl FastLane lysate in subsequent real-time RT-PCR?
FAQ ID -1480
Are FlexiPlate siRNAs available at a higher final concentration than 10 µM?
FAQ ID -1476
Can different amounts of siRNA be ordered on one FlexiPlate?
FAQ ID -1473
Can 2 µl reaction volumes be used with QuantiFast PCR Kits?
FAQ ID -1440
Do the QuantiTect Primer Assays work with QuantiFast SYBR Green Kits?
FAQ ID -1439
Do special settings have to be used for QuantiFast PCR Kits on the Eppendorf Mastercycler ep realplex?
FAQ ID -1437
What information is required to reorder specific siRNAs from a siRNA Set in FlexiPlate format?
FAQ ID -1463
What are the turnaround times for FlexiPlate siRNA orders?
FAQ ID -1478
How much time will be saved when switching from standard cycling to fast cycling with QuantiFast Kits?
FAQ ID -1438
How many reactions can I perform with the new QuantiFast Kits for real-time PCR?
FAQ ID -1425
If FlexiPlate siRNA was accidentally redissolved in siRNA Suspension Buffer instead of water, resulting in a 2x buffer, would it affect performance of the siRNAs?
FAQ ID -1477
How do you achieve fast cycling, yet still deliver the same performance in PCR as that achieved with standard cycling?
FAQ ID -1430
Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube?
FAQ ID -1415
Why do QuantiFast SYBR Green PCR Kits require such a high primer concentration?
FAQ ID -1443
What QuantiFast Kit should be used on the Eppendorf Mastercycler ep realplex?
FAQ ID -1436
What is the detection limit of the QuantiFast Kits for real-time PCR?
FAQ ID -1432
Which kind of 96 and 384-well plates are available for FlexiPlate siRNAs?
FAQ ID -1459
Which cell lines have been tested with FastLane Cell RT-PCR Kits?
FAQ ID -1485
Can I adjust the ROX concentration in the QuantiFast master mix?
FAQ ID -1427
Can I follow the reaction setup for probe-based assays described in Appendix D of the FastLane Cell RT-PCR Handbook using 384-well plates?
FAQ ID -1482
Can FlexiPlate siRNAs be ordered in plates other than the standard NUNC 96 well V bottom plates?
FAQ ID -1458
Can fast cycling be carried out with QuantiFast Probe PCR Kits and FRET probes?
FAQ ID -1433
Can FlexiPlate siRNA be ordered in solution on dry-ice?
FAQ ID -1474
How do QuantiFast PCR Kits compare to QuantiTect PCR Kits for quantitative real-time PCR?
FAQ ID -1441
How can I get software updates for the QIAcube?
FAQ ID -1413
Do you offer trial-kit sizes for the new QuantiFast Kits?
FAQ ID -1429
Can the QIAcube rotor adapters be autoclaved?
FAQ ID -1409
Can QuantiFast PCR Kits be used on real-time PCR instruments without fast cycling options?
FAQ ID -1428
Does the high primer concentration required by QuantiFast SYBR Green PCR Kits impair annealing specificity?
FAQ ID -1444
Does the master mix in the QuantiFast Kits contain dUTP to allow UNG treatments?
FAQ ID -1431
Can the QuantiFast Probe PCR +ROX Vial mastermix be stored long-term after adding ROX dye?
FAQ ID -1435
What are important points regarding the genomic DNA wipeout buffer in the QuantiFast Probe RT-PCR Plus Kit?
FAQ ID -2359
Will the sequence of the QuantiFast Probe Assays be provided?
FAQ ID -2369
Why are samples centrifuged at 4°C after addition of chloroform to the QIAzol lysates in following kits: RNeasy Lipid Tissue Mini/Midi kits, RNeasy Microarray Tissue Mini kit, RNeasy Plus Universal Mini/Midi kit, RNeasy 96 Universal Tissue kit, miRNeasy Mini kit, miRNeasy Micro kit, miRNeasy 96 kit and miRNeasy Serum/Plasma kit?
FAQ ID -2345
Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)?
FAQ ID -2366
Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA?
FAQ ID -2365
What are the main differences between the qBiomarker PCR Arrays and the RT2 Profiler PCR Arrays?
FAQ ID -2438
What are the recommended storage conditions of the QuantiFast Probe RT-PCR Plus Kit components?
FAQ ID -2355
What are the differences between the existing QuantiFast Probe RT-PCR Kit and the new Plus Kit?
FAQ ID -2358
What is the concentration of the primers and the probe in a reaction using the QuantiFast Probe Assays?
FAQ ID -2370
What is the average length of a QuantiFast Probe Assay?
FAQ ID -2374
Is the RNeasy Plus Universal Mini Kit suited for adipose, brain, and other fatty tissues?
FAQ ID -2343
Is there a size limit to the protein I can crystallize with the NexTal Cubic Phase products?
FAQ ID -2331
Is the T790M mutation included in the EGFR PCR kit?
FAQ ID -2314
Is it necessary to perform calibration steps for the use of the MAX dye in duplex RT-PCR experiments on different cyclers?
FAQ ID -2373
Is there also a miRNeasy Plus Universal Kit available?
FAQ ID -2346
I use cultured cells as starting material. Can I also use the RNeasy Plus Universal Kit?
FAQ ID -2342
I would like to order the QuantiFast Probe Assays without master mix. Is this possible?
FAQ ID -2360
My ADAPT report states "Run Invalid" and does not display any sample results. Why is this?
FAQ ID -2306
Is V600E the only mutation that can be detected with the codon 600 assay in the BRAF Pyro Kit?
FAQ ID -2382
Is the yield and quality of RNA and DNA purified with the AllPrep DNA/RNA FFPE Kit comparable to yield and quality of nucleic acids purified separately with other methods.
FAQ ID -2349
Which purification kits are recommended for the Pyro kits?
FAQ ID -2380
Which kit, the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, do you recommend for transfection?
FAQ ID -2320
Which probe labels are available for the QuantiFast Probe Assays?
FAQ ID -2361
What should be the concentration of my protein with the NexTal Cubic Phase products?
FAQ ID -2325
What is the maximum plasmid size which I can use with the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit?
FAQ ID -2316
What plug-ins are available for the Pyrosequencing kits?
FAQ ID -2381
Which crystallization solutions are compatible with lipidic cubic phase crystallization?
FAQ ID -2418
Which deparaffinization method can I use with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2352
What species are QuantiTect Probe Assays offered for?
FAQ ID -2367
Can the insertions in Exon 20 be detected with the EGFR Pyro Kit?
FAQ ID -2383
Is it also possible to isolate miRNA with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2348
Can I use the RNeasy Plus Universal Mini Kit for all types of tissue?
FAQ ID -2338
Can the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit be used for low-copy number plasmids?
FAQ ID -2317
Can the QuantiFast Probe Assay detect short fragmented RNA as well as longer fragments of RNA?
FAQ ID -2364
Can negative crossing threshold (CT) values be negative when using the KRAS and EGFR PCR kits?
FAQ ID -2300
Can QuantiFast Probe Assays be ordered offline via e-mail or fax?
FAQ ID -2368
For the the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, can plates be partially used?
FAQ ID -2319
For which applications can the RNA isolated with the RNeasy Plus Universal Kit also be used?
FAQ ID -2339
Does the KRAS PCR kit cover the codon 61 mutation?
FAQ ID -2308
Are the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit suited for short hairpin vectors?
FAQ ID -2318
Are the QuantiFast Probe Assays hydrolysis or FRET probes?
FAQ ID -2363
Are the buffers in the RNeasy QIAcube kit delivered in QIAcube buffer bottles?
FAQ ID -2334
Are QIAshredder spin columns delivered with the RNeasy Mini QIAcube Kit?
FAQ ID -2333
Can I do singleplex PCR with the QuantiFast Probe Assays?
FAQ ID -2362
Can I use more than 10ng DNA for the PCR reaction for the Pyro kits?
FAQ ID -2379
Are the QuantiFast Probe Assays wet-lab validated?
FAQ ID -2375
At what temperature can I perform the experiment with the NexTal Cubic Phase kits?
FAQ ID -2323
How much can the mixed standard ?Ct values vary in the KRAS PCR kit?
FAQ ID -2307
How many samples can be processed per RNeasy Mini QIAcube kit?
FAQ ID -2332
How much DNA/RNA can be isolated with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2353
How many freeze-thaw cycles can the master mix contained in the QuantiFast Probe RT-PCR Plus Kit tolerate?
FAQ ID -2357
How pure should the chloroform used with the RNeasy Plus Universal Mini Kit be?
FAQ ID -2344
How much sample material can I use for DNA/RNA purification with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2351
How much time can be saved when using the RNeasy Mini QIAcube Kit?
FAQ ID -2335
How does the QuantiFast Probe RT-PCR Plus Kit eliminate genomic DNA contamination?
FAQ ID -2354
How do I analyze the data for the particular iPSC qBiomarker Arrays I have ordered?
FAQ ID -2440
How are the genes in each iPSC qBiomarker PCR Array selected?
FAQ ID -2439
How can I adjust the ROX concentration in the QuantiFast Probe RT-PCR Plus master mix?
FAQ ID -2356
How do I harvest the crystals from meso phase? Are special tools needed with the NexTal Cubic Phase products?
FAQ ID -2330
What is the advantage of EpiTect MethyLight PCR Kits over QuantiTect Probe PCR Kits for methylation studies?
FAQ ID -2010
What is the advantage of using the Type-it Microsatellite PCR Kit?
FAQ ID -2059
Back to top