Frequently Asked Questions - FAQ



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You are not authorized to download the resource Sort options アルファベット順関連順 3315-What is the concentration of the reconstituted QuantiTect Primer Assay (200)? 表示 3322 - What is the concentration of the reconstituted miScript Primer Assay (100) and miScript Precursor Assay (100) tubes? 表示 3317 - Do you have a protocol for isolation of genomic DNA from cell cultures using the QIAamp cador Pathogen Mini Kit? 表示 3318 - What guidelines should I follow in designing primers for use with the EpiTect MSP Kit? 表示 3320 - What is the sensitivity of the Type-it HRM PCR Kit? 表示 3321 - What is the purpose of the C. elegans miR-39 spike-in control in the miRNeasy Serum/Plasma kit? 表示 3318 - What is the preferred amplicon size for performing PCR on DNA amplified with the EpiTect Whole Bisulfitome Kit? 表示 3305 - What is the minimal amount of starting material required for using the SBTexcellerator HLA Kits? 表示表示表示表示 3306 - What is the optimal sample DNA concentration for the Investigator ESSplex SE Plus Kit? 表示 3304 - What is the maximum DNA input volume that can be used in the Investigator ESSplex SE Plus Kit? 表示 3310 - Which settings for the filter set Any5Dye must be adjusted before running the spectral calibration for Investigator ESSplex SE Plus Kit? 表示 3308 - Where can I find the template files for the Investigator ESSplex SE Plus Kit? 表示 3309 - Which filter set should be used with Investigator ESSplex SE Plus Kit? 表示 What additional equipment is needed to purify RNA using the PAXgene 96 Blood RNA Kit? FAQ ID -499 表示 3300 - Can isopropanol or methanol be used in place of ethanol in RNeasy kits? 表示 3301 - Do you have any information or guidelines regarding the choice of reference genes for real-time PCR? 表示 3302 - How long can I store my purified RNA? 表示 3210 - Can the 4-16K Centrifuge be used with a 208 V outlet? 表示 3211 - What is the maximum speed of the plate rotor 2 x 96 in the 4-16 or 4-16K? 表示 3212 - What is the minimum size of DNA fragment that can be isolated with QIAamp DNA Mini kit? 表示 3299-Can Buffer ER from the Endofree Plasmid kits be used instead of the Buffer ETR in QIAGEN Plasmid Plus kits? 表示 3213 - How much ethanol should be added to Buffer AP3/E (renamed to Buffer AW1) and Buffer AW (renamed to Buffer AW2) in the DNeasy Plant kit? 表示 3202 - Can GeneRead rRNA depletion kit be used to deplete rRNA from miRNA isolated using miRNeasy kit? 表示 3200 - What is the Maximum/Minimum amount of RNA that can be used with GeneRead rRNA depletion kit? 表示 3201 - What quality of RNA can be used with the GeneRead rRNA depletion kit? 表示 3204 - How strict is the purification cut-off with GeneRead Size Selection kit? 表示 3203 - Is the RNeasy MinElute cleanup kit required for the GeneRead rRNA depletion kit? 表示 3205 - Can GeneRead Library preparation kits be used on species other than human mouse and rat, for example, plant species and microbial species? 表示 3206 - Can our GeneRead Library preparation kits be used with the SoLiD Platform? 表示 3248 - What is the difference between sponge, cubic and lamellar phase? 表示 3254 - When using the Nextal Cubic Phase products how do I know in which phase the sample is? What will I see? 表示 3255 - When using the NexTal Cubic Phase products, should I work with diluted or undiluted screens? 表示 3227 - In which solution can I keep my protein with the NexTal Cubic Phase kits? 表示 3225 - How pure should my protein be for the NexTal CubicPhase kits? 表示 3217 - How long can I store my purified RNA? 表示 3292-How do I start using the NeXtal CubicPhase kits? 表示 3295-What is the difference between SBTexcellerator HLA Core, Extended and Full Kits? 表示 3240 - What are the main differences between the qBiomarker PCR Arrays and the RT2 Profiler PCR Arrays? 表示 3237 - I would like to order the QuantiFast Probe Assays without master mix. Is this possible? 表示 3233 - Is the yield and quality of RNA and DNA purified with the AllPrep DNA/RNA FFPE Kit comparable to yield and quality of nucleic acids purified separately with other methods. 表示 3265 - Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)? 表示 3298-Will the sequence of the QuantiFast Probe Assays be provided? 表示 3262 - Which thermal cyclers are validated for use with the Investigator Nonaplex ESS Kit? 表示 3263 - Why are samples centrifuged at 4°C after the addition of chloroform when using the RNeasy Plus Universal Mini Kit? 表示 3241 - What are the recommended storage conditions of the QuantiFast Probe RT-PCR Plus Kit components? 表示 3264 - Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA? 表示 3238 - What are important points regarding the genomic DNA wipeout buffer in the QuantiFast Probe RT-PCR Plus Kit? 表示 3245 - What is the concentration of the primers and the probe in a reaction using the QuantiFast Probe Assays? 表示 3239 - What are the differences between the existing QuantiFast Probe RT-PCR Kit and the new Plus Kit? 表示 3247 - What is the correct matrix to use with the Investigator Nonaplex ESS Kit? 表示 3243 - What does the Investigator Nonaplex ESS Kit contain? 表示 3244 -What is the average length of a QuantiFast Probe Assay? 表示 3231 - Is there a size limit to the protein I can crystallize with the NexTal Cubic Phase products? 表示 3228 - Is it also possible to isolate miRNA with the AllPrep DNA/RNA FFPE Kit? 表示 3232 - Is the RNeasy Plus Universal Mini Kit suited for adipose, brain, and other fatty tissues? 表示 3229 - Is it necessary to perform calibration steps for the use of the MAX dye in duplex RT-PCR experiments on different cyclers? 表示 3236 - I use cultured cells as starting material. Can I also use the RNeasy Plus Universal Kit? 詳細を表示 Is there also a miRNeasy Plus Universal Kit available? 表示 3261 - Which purification kits are recommended for the therascreen Pyro kits? 表示 3258 - Which filter set and settings should be used with the Investigator Nonaplex ESS Kit? 表示 3260 - Which purification kits are recommended for the Pyro kits? 表示 3259 - Which probe labels are available for the QuantiFast Probe Assays? 表示 3296-Why does the A peak reduction factor have to de set to 0.86 for the codon 61 assay in the therascreen NRAS Pyro Kit? 表示 What plug-ins are available for the therascreen Pyro kits? FAQ ID - 3251 表示 3249 - What is the optimal DNA volume and concentration for the Investigator Nonaplex ESS Kit? 表示 What plug-ins are available for the Pyrosequencing kits? FAQ ID - 3250 表示 3252 - What should be the concentration of my protein with the NexTal Cubic Phase products? 表示 3256 - Which crystallization solutions are compatible with lipidic cubic phase crystallization? 表示 3253 - What species are QuantiTect Probe Assays offered for? 表示 3257 - Which deparaffinization method can I use with the AllPrep DNA/RNA FFPE Kit? 表示 Can the insertions in Exon 20 be detected with the therascreen EGFR Pyro Kit? 表示 Can the insertions in Exon 20 be detected with the EGFR Pyro Kit? FAQ ID - 3279 表示 3277 - Can I use the RNeasy Plus Universal Mini Kit for all types of tissue? 表示 Can QuantiFast Probe Assays be ordered offline via e-mail or fax? FAQ ID - 3278 表示 3283-Do you have any information or guidelines regarding the choice of reference genes for real-time PCR? 表示 3285-How are the genes in each iPSC qBiomarker PCR Array selected? 表示 3284-For which applications can the RNA isolated with the RNeasy Plus Universal Kit also be used? 表示 Can the QuantiFast Probe Assay detect short fragmented RNA as well as longer fragments of RNA? 表示 Do the RNeasy Plus Universal Mini/Midi kits contain both wash buffers, RW1 and RWT? FAQ ID - 3282 表示 3270 - Are the primer sets different for the Investigator Nonaplex ESS Kit and ESSplex SE where the loci are the same? 表示 Are the QuantiFast Probe Assays hydrolysis or FRET probes? FAQ ID - 3271 表示 Are panels and bin sets available for use with GeneMapper ID and GeneMapper ID-X? FAQ ID - 3267 表示 3275 - Can I use more than 10ng DNA for the PCR reaction for my therascreen Pyro Kits? 表示 Can I use more than 10ng DNA for the PCR reaction for the Pyro kits? FAQ ID - 3276 表示 3274 - Can I do singleplex PCR with the QuantiFast Probe Assays? 表示 3273 - At what temperature can I perform the experiment with the NexTal Cubic Phase kits? 表示 3272 - Are the QuantiFast Probe Assays wet-lab validated? 表示 3222 - How much DNA/RNA can be isolated with the AllPrep DNA/RNA FFPE Kit? 表示 3218 - How many fragments are contained within the size standard BTO in the Investigator Nonaplex ESS Kit? 表示 3219 - How many freeze-thaw cycles can the master mix contained in the QuantiFast Probe RT-PCR Plus Kit tolerate? 表示 3220 - How many PCR cycles should I use for the Investigator Nonaplex ESS Kit? 表示 3226 - How pure should the chloroform used with the RNeasy Plus Universal Mini Kit be? 表示 3223 - How much sample material can I use for DNA/RNA purification with the AllPrep DNA/RNA FFPE Kit? 表示 3224 - How much time can be saved when using the RNeasy Mini QIAcube Kit? 表示 3289-How does the QuantiFast Probe RT-PCR Plus Kit eliminate genomic DNA contamination? 表示 3290-How do I analyze the data for the particular iPSC qBiomarker Arrays I have ordered? 表示 3288-How does the gDNA eliminator solution of the RNeasy Plus Universal Kit work? 表示 3294-How long can I store my purified DNA/RNA isolated with the AllPrep DNA/RNA FFPE kit? 表示 3286-How can I adjust the ROX concentration in the QuantiFast Probe RT-PCR Plus master mix? 表示 3291-How do I harvest the crystals from meso phase? Are special tools needed with the NexTal Cubic Phase products? 表示 3246 - What is the correct matrix to use with Investigator ESSplex SE Plus Kit? 表示 3297-Why does the PCR for HLA-DRB4 result in 2 amplicons of different size? 表示 3266 - Why are two different exon 2 forward sequencing primers used for the SBTexcellerator HLA-DRB1 Kit? 表示 How can I find out if my gene of interest is expressed in a specific tissue type or cell line? FAQ ID - 3214 表示 3215 - What is the starting material for SBTexcellerator HLA Kits? 表示 Can I use genomic DNA from fixed samples with qBiomarker Copy Number PCR Arrays? FAQ ID — 3416 表示 Can I use amplified genomic DNA with qBiomarker Copy Number PCR Arrays? FAQ ID — 3415 表示 Do the samples require pretreatment before starting the manual protocol or before loading onto the QIAcube instrument? FAQ ID - 3479 表示 What are the expected yields of RNA from PAXgene Blood RNA Tubes isolated with the PAXgene Blood miRNA kit? FAQ ID - 3481 表示 Do you have stability data for RNA isolated with PAXgene miRNA Kit? FAQ ID - 3480 表示 Can the PAXgene Blood DNA Tubes be stored at 4°C? FAQ ID - 3493 表示 How should I thaw frozen PAXgene Blood DNA Tubes? FAQ ID - 3495 表示 Can the PAXgene Blood DNA Tube be frozen? If yes, at what temperatures and for how long? FAQ ID - 3491 表示 Can mitochondrial DNA be isolated with the PAXgene Blood DNA System? FAQ ID - 3498 表示 3497 - What is the expected purity and size of DNA extracted using the PAXgene Blood DNA kit? FAQ ID - 3497 表示 Does the DNA extracted using the PAXgene Blood DNA System contain RNA? FAQ ID - 3499 表示 Can I multiplex the Microbial DNA qPCR Assays? FAQ ID — 3408 表示 Can I measure virulence factor gene expression? FAQ ID — 3405 表示 What sample types can I test on qBiomarker Copy Number PCR Arrays? FAQ ID — 3423 表示 What is the sensitivity for the Microbial DNA qPCR kits? FAQ ID — 3409 表示 Which probe labels are available for the Microbial DNA qPCR Assays? FAQ ID — 3407 表示 Are the Microbial DNA qPCR Assays wet-lab verified? FAQ ID — 3406 表示 Can I measure antibiotic resistance gene expression? FAQ ID — 3404 表示 What sequences are used to design the Microbial DNA qPCR Assays? FAQ ID — 3403 表示 Are the assays species-specific? FAQ ID — 3400 表示 Why is the copy number call lower than expected? FAQ ID — 3413 表示 Will qBiomarker Copy Number Assays work with heterogeneous samples like mixtures of tumor and normal tissue? FAQ ID — 3419 表示 Why do qBiomarker Copy Number PCR Arrays have assays in quadruplicate? FAQ ID — 3414 表示 How should I analyze the data generated from a qBiomarker Copy Number PCR Array experiment? FAQ ID — 3421 表示 What could have gone wrong if the CT values are unusually high for all wells in a sample? FAQ ID — 3412 表示 What qPCR mastermix should I use with the qBiomarker Copy Number PCR Arrays and Assays FAQ ID — 3422 表示 How much DNA should I use on a qBiomarker Copy Number PCR Array? FAQ ID — 3417 表示 How much DNA should I use in a qBiomarker Copy Number PCR Assay? FAQ ID — 3418 表示 How do you choose which assays to include on the arrays? FAQ ID — 3420 表示 What testing should be performed in order to assess the quality of a DNA sample? FAQ ID — 3424 表示 How many samples can be processed with the PAXgene Blood miRNA Kit? FAQ ID - 3477 表示 Why have the hazard symbols accompanying products changed? FAQ ID - 3430 表示 Why is a blood collection set required to be used with the PAXgene Blood RNA tube? FAQ ID - 3459 表示 As part of my RT procedure I routinely heat an aliquot of the eluate prior to the RT reaction. Do I still need to heat the whole eluate after the RNA preparation? FAQ ID - 3468 表示 Is there any chance of the PAXgene Blood RNA tube reagent going back into the patient's arm? FAQ ID - 3460 表示 I need to buy a new computer for my PyroMark Q96. Can I reinstall the PyroMark Q96 software on my new computer or do I need to purchase a new license? FAQ ID - 3472 表示 Can I reinstall the PyroMark Q24 software on my new computer or following an operating system upgrade, or do I need to purchase a new license? FAQ ID - 3473 表示 Can the PAXgene Blood DNA Tubes be stored at higher temperatures? FAQ ID - 3494 表示 What are the expected yields of RNA from PAXgene Blood RNA Tubes using the QIAsymphony PAXgene Blood RNA Kit? FAQ ID - 3475 表示 What additional equipment is needed to purify RNA using the PAXgene 96 Blood RNA Kit? FAQ ID - 3474 表示 Is it possible to isolate genomic DNA or viral nucleic acids with the QIAsymphony PAXgene Blood RNA protocol? FAQ ID - 3476 表示 2. Are there any special considerations for the collection of blood using PAXgene Blood DNA Tubes CE (e.g., use of a blood collection set or discard tube, positioning of the tube, issues with short draws, etc.)? FAQ ID - 3485 表示 Are the PAXgene Blood DNA Tubes sterile? FAQ ID - 3487 表示 What is the additive in PAXgene Blood DNA Tubes FAQ ID - 3486 表示 What is the maximum number of samples for the QIAcube variant of the PAXgene Blood miRNA protocol for a single run? FAQ ID - 3482 表示 Do I have to use a blood collection set with the PAXgene Blood DNA Tube? FAQ ID - 3484 表示 Is it possible to isolate genomic DNA or viral nucleic acids with PAXgene Blood miRNA protocol? FAQ ID - 3483 表示 How should blood samples drawn into PAXgene Blood DNA Tubes be shipped? FAQ ID - 3489 表示 How long can the PAXgene Blood DNA Tube be kept at room temperature? What is room temperature? FAQ ID - 3492 表示 Which sample types can be processed using the PAXgene Blood miRNA protocols? FAQ ID - 3478 表示 What is the shelf life of the PAXgene Blood DNA Tube prior to blood collection? FAQ ID - 3488 表示 How should blood samples drawn into the PAXgene Blood DNA Tubes be stored? FAQ ID - 3490 表示 Can the miScript II RT Kit be used to quantify piRNAs? FAQ ID - 3432 表示 Can the miScript Plant RT Kit be used to quantify piRNA expression? FAQ ID - 3434 表示 Can the miScript Plant RT Kit be used to quantify animal miRNAs? FAQ ID - 3433 表示 Does the miScript Plant RT Kit contain controls? FAQ ID - 3435 表示 What is the composition of the 5x miScript Plant RT Buffer? FAQ ID - 3437 表示 Are any of the miScript II RT Kit components interchangeable with the miScript Plant RT Kit components? FAQ ID - 3436 表示 What should I use to isolate total RNA from plant tissues and what is the maximum recommended amount of tissue input? FAQ ID - 3438 表示 Are miScript Primer Assays available for plant mature miRNAs? FAQ ID - 3439 表示 Are plant miScript Primer Assay designs wet-bench validated? FAQ ID - 3440 表示 Can you describe the adaptor found in the 10x miScript Plant Adaptor? FAQ ID - 3442 表示 Are miScript Primer Assay included in miScript miRNA PCR Arrays the same primers as the miScript Primer Assays available in individual tubes? FAQ ID - 3441 表示 What is the amplicon size of PCR products generated with the miScript Plant qPCR System? FAQ ID - 3443 表示 What is the recommended RNA and RNA input range for the miScript Plant RT Kit? FAQ ID - 3446 表示 Can cDNA synthesized with the miScript Plant RT Kit be used in real-time PCR with TaqMan® MicroRNA Assays? FAQ ID - 3445 表示 Can the PAXgene RNA stabilization solution/blood mixture from the PAXgene Blood RNA Tube be disposed of by adding bleach? FAQ ID - 3469 表示 Can I scale up reagents if I want to use more sample with the MagAttract HMW DNA Kit (cat. no. 67563)? FAQ ID - 3448 表示 How can I calculate the number of bacterial cells that are present in a sample using the Microbial DNA qPCR Assays? FAQ ID — 3411 表示 What is the difference between the Microbial DNA qPCR Assays and the QuantiFast Pathogen +IC Kits? FAQ ID — 3410 表示 What RNA yields are expected with the PAXgene Blood RNA kit? FAQ ID - 3464 表示 Can the PAXgene 96 Blood RNA Kit be used for diagnostic or prognostic procedures? FAQ ID - 3471 表示 What are possible reasons for blood draws with lower than expected blood volume? FAQ ID - 3462 表示 How long can the PAXgene Blood RNA tubes be kept frozen at –20°C or –70°C? FAQ ID - 3463 表示 Can the PAXgene Blood RNA System be used to isolate DNA? FAQ ID - 3466 表示 Can the PAXgene Blood RNA System be used to isolate viral RNA? FAQ ID - 3465 表示 Can the PAXgene Blood RNA System be used with animal blood samples? FAQ ID - 3461 表示 Can I use DNA isolated from an AllPrep DNA/RNA Kit with qBiomarker Copy Number PCR Arrays and Assays FAQ ID — 3425 表示 What is the Globally Harmonized System of Classification and Labelling of Chemicals (GHS) and what is the impact for me as a customer? FAQ ID - 3428 表示 What transfection method should I use with the SureSilencing shRNA Plasmids? FAQ ID -2901 表示 What are the software requirements to run the QIAlink software package? FAQ - 3426 表示 What is the data analysis method, and where can I find it for the qBiomarker Somatic Mutatation PCR arrays? FAQ ID -2915 表示 What is the nature and target of the Internal Control supplied with Certal Residual DNA Detection Kits? FAQ ID -2974 表示 What is the principle for qBiomarker Somatic Mutation PCR Array data analysis? FAQ ID -2916 表示 What is the label of the probe that is used in the Internal Control Assay to detect the Internal Control supplied with Certal Residual DNA Detection Kits? FAQ ID -2976 表示 Were all assays on the qBiomarker Somatic Mutation PCR Arrays bench-validated? FAQ ID -2912 表示 What are the advantages of RT-PCR based somatic mutation PCR arrays and assays compared to other platforms? FAQ ID -2921 表示 What are the control features on the qBiomarker Somatic Mutation PCR Arrays? FAQ ID -2914 表示 What happens if the starting volume is too low when using QIAsymphony mericon Bacteria Kit? FAQ ID -2962 表示 What happens if the starting volume is too high when using QIAsymphony mericon Bacteria Kit? Can spillage occur when the sample is initially transferred? FAQ ID -2963 表示 What effects can be predicted with poor sample quality? How robust is the platform with questionable sample quality (such as FFPE samples)? FAQ ID -2918 表示 What elution formats can be used for the QIAsymphony PAXgene Blood RNA protocol? FAQ ID -2986 表示 What elution formats can be used for the QIAsymphony DSP Virus/Pathogen protocols? FAQ ID -2937 表示 When should I choose EasyXpress Disulfide Insect Kits? FAQ ID -2971 表示 Which elution volumes can be used for the QIAsymphony DSP Virus/Pathogen protocols? FAQ ID -2938 表示 What sample volumes can be used with the tissue HC and LC protocols on the QIAsymphony SP? FAQ ID -2992 表示 What types of templates can be used for expression of antibody fragments? FAQ ID -2964 表示 Why do I have to use an ACS (Assay Control Set)? FAQ ID -2933 表示 Which kit size should be used for each QIAsymphony DSP Virus/Pathogen protocol? FAQ ID -2930 表示 Will mitochondrial DNA also be isolated using the DNA tissue protocols and QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP? FAQ ID -2991 表示 Which sample types can be processed using the QIAsymphony PAXgene Blood RNA Protocol? FAQ ID -2981 表示 What pathways and diseases are currently covered for the qBiomarker Somatic Mutation PCR Arrays? FAQ ID -2922 表示 Why does the template for EasyXpress Disulfide Insect Kits need to have a signal sequence (such as the mellitin signal sequence as provided by the EasyXpress Linear Template Fab Kit primers)? FAQ ID -2967 表示 What sample input formats can be used for the QIAsymphony DSP Virus/Pathogen protocols? FAQ ID -2936 表示 What is the starting volume for the QIAsymphony mericon Bacteria Kit? FAQ ID -2956 表示 What is the Limit of Detection (LOD) for an ELISArray Kit? FAQ ID -2907 表示 What is the sensitivity of the assays contained on the qBiomarker Somatic Mutation PCR Arrays? FAQ ID -2910 表示 What is the linear range of the Multi-Analyte ELISArray Kits? FAQ ID -2909 表示 What are the expected DNA yields from tissue processed on the QIAsymphony SP? FAQ ID -2990 表示 What is the maximum number of GOIs that I can detect with the Type-it CNV Probe Master Mix in the same qPCR reaction? FAQ ID -2998 表示 What are the recommended lysis times for tissue samples on the QIAsymphony SP? FAQ ID -2993 表示 What are the technological principles behind somatic mutation PCR arrays and assays? FAQ ID -2924 表示 What PCR machine do I need to use to run such a qBiomarker Somatic Mutation PCR Arrays? FAQ ID -2917 表示 What method(s) do you recommend as an alternative way to validate the mutations identified on the somatic mutation PCR arrays? FAQ ID -2920 表示 What sample types can I test on the qBiomarker Somatic Mutation PCR Arrays? FAQ ID -2919 表示 What sample volumes can be processed using the QIAsymphony DSP Virus/Pathogen protocols? FAQ ID -2929 表示 What sample types should be processed with the low content (LC) and high content (HC) protocols for DNA extraction on the QIAsymphony SP? FAQ ID -2989 表示 What samples can the QIAsymphony mericon Bacteria Kit be used for? FAQ ID -2958 表示 What sample types can be processed using the QIAsymphony DSP Virus/Pathogen protocols? FAQ ID -2928 表示 Do you have stability data for RNA isolated with the QIAsymphony PAXgene Blood RNA Kit? FAQ ID -2983 表示 What sample types can be processed using the tissue protocols and the QIAsymphony DSP DNA Mini Kit? FAQ ID -2925 表示 Do you need to pre-treat food samples before DNA isolation using the QIAsymphony mericon Bacteria Kit on the QIAsymphony SP? FAQ ID -2957 表示次ページ Back to top Contact QIAGEN Global contacts Technical Service Customer Care