Frequently Asked Questions - FAQ



FAQ Bookmark シェア more.. You are not authorized to download the resource Sort options アルファベット順関連順 Can the miScript Single Cell qPCR Kit be used to quantify piRNA expression? FAQ ID - 3567 表示 Can the miScript Single Cell qPCR Kit be used with plant cells? FAQ ID - 3568 表示 Are any of the miScript Single Cell qPCR Kit components interchangeable with miScript II RT Kit, miScript Plant RT Kit, miScript PreAMP PCR Kit, or miScript Microfluidics PreAMP Kit components? FAQ ID - 3569 表示 Should miScript PreAMP Primer Mixes be used in conjunction with the miScript Single Cell qPCR Kit? FAQ ID - 3570 表示 Is the cDNA cleanup step in the miScript Single Cell qPCR Kit workflow absolutely required? FAQ ID - 3571 表示 I have accidentally pipetted beads when removing the eluate after cDNA cleanup. What should I do? FAQ ID - 3572 表示 Does the miScript Single Cell qPCR Kit contain controls? FAQ ID - 3574 表示 When performing real-time PCR quality control associated with the miScript Single Cell qPCR Kit, is it necessary to perform control experiments for all individual samples/cells? FAQ ID - 3576 表示 I do not see any miRNA expression for my miRNAs of interest after performing single cell miRNA expression analysis with the miScript Single Cell qPCR Kit and miScript miRNA PCR Arrays or miScript Primer Assays. What could be the cause? FAQ ID - 3577 表示 What is the amplicon size of PCR products generated with the miScript Single Cell qPCR System? FAQ ID - 3578 表示 What is the dissociation curve temperature of PCR products generated with the miScript Single Cell qPCR System? FAQ ID - 3579 表示 When performing real-time PCR quality control associated with the miScript Single Cell qPCR Kit, is it necessary to perform control experiments for all individual samples/cells? FAQ ID - 3575 表示 Do you recommend normalizing single cell miRNA expression data to a “housekeeping” gene or miRNA? FAQ ID - 3580 表示 How should data be analyzed when the miScript Single Cell qPCR Kit is used with miScript miRNA PCR Arrays or miScript Primer Assays? FAQ ID - 3581 表示 What single cell isolation methods are recommended for use with the miScript Single Cell qPCR Kit? FAQ ID - 3582 表示 How would I provide information on the QuantiTect Primer assays in publications when the sequence is confidential? FAQ ID - 3593 表示 In which tubes is clot detection possible on QIAsymphony? FAQ ID - 3589 表示 How can I create a report/logfile on my QIAsymphony SP system? FAQ ID - 3594 表示 Is it possible to measure the proteolytic activity of proteins on the surface of exosomes that were isolated with ExoEasy? FAQ ID - 3599 表示 Which barcodes on sample tubes are accepted by the QIAsymphony SP? FAQ ID - 3598 表示 Can I change the language on my QIAsymphony SP system? FAQ ID - 3595 表示 Can I use blood, cells or bacteria with the QIAamp Fast DNA Tissue Kit? FAQ ID - 3587 表示 Can the Rotor-Gene Q be connected to LIMS? FAQ ID - 3596 表示 Can the REPLI-g Mitochondrial DNA Kit be used for species other than humans? FAQ ID - 3584 表示 Which Microbial qPCR Mastermix should I use? FAQ ID — 3395 表示 What are the storage conditions for the Microbial DNA qPCR products? FAQ ID — 3394 表示 3327 - How often should the o-ring for the pipettor tip-adapter be changed on the QIAcube? 表示 3326 - Which QIAcube standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet? 表示 3328 - Can the Sample Tubes CB and the Sample Tubes RB be used interchangeably? 表示 3330 - Is there any contamination from E. coli genomic DNA in the polymerase provided with the Repli-G Single Cell Kit? 表示 3329 - When using REPLI-g, if the starting material is a single sorted cell, could mitochondrial DNA (mtDNA) be amplified? 表示 3331 - Should the REPLI-g amplified mtDNA be cleaned up before sequencing? 表示 3332 - Is there a method that can distinguish between non-amplified DNA and REPLI-g amplified DNA? 表示 3333 - When using REPLI-g, what is the purpose of the DTT added to the denaturation buffer in the cells/blood protocol? 表示 What sample types can be tested on the arrays/assays? FAQ ID — 3399 表示 What are the minimum sample requirements for Microbial DNA qPCR kits? FAQ ID — 3393 表示 Can I use the Microbial DNA-Free Water and Microbial qPCR Mastermix if they have been opened more than 3 times? FAQ ID — 3398 表示表示 3353 - What is the composition of the elution buffer in the Ni-NTA Fast Start Kit? FAQ ID - 3353 表示 3352 - What are the size ranges of Ni-NTA particles? FAQ ID - 3352 表示 3354 - What type and amount of resin is packed into the Ni-NTA Spin columns from the Ni-NTA Spin Kit? FAQ ID - 3354 表示 3341 - How are the EpiTect DNA controls produced and tested? FAQ - 3341 表示 3351 - What is the upper limit for protein size that can bind to Ni-NTA agarose resin? FAQ ID - 3351 表示 3344 - What is the difference between the EpiTect Plus DNA Bisulfite kit compared to the classic kit? FAQ - 3344 表示 3345 - What is the difference between the high and low concentration EpiTect Bisulfite Conversion protocol? FAQ - 3345 表示 3311 - Can QuickLyse miniprep kits be used with QIAVac 24 Plus? 表示 3313 - What is the endotoxin level for the QIAGEN Plasmid Plus kits? 表示 3312 - For DirectPrep 96 miniprep, is it possible to use a half plate and save the other half for later prep? 表示 3314-What is the concentration of RNase A sold separately? 表示 3316-What is the concentration of the reconstituted QuantiFast Probe Assay? 表示表示 3325 - What are the catalog numbers for the packing materials for the QIAcube? 表示 3324 - Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube? 表示 3335 - Can I use the DNA directly from enzymatic reaction for Epitect Bisulfite treatment with the Epitect Bisulfite kit (cat. no. 59104)? 表示 3336 - Can I use the linearized DNA for Epitect Bisulfite treatment with the Epitect Bisulfite kit? 表示 3339 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) negatively impact subsequent NGS sequencing? 表示 3337 - Which method is used for QIAGEN sequencing service? 表示 3338 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) have 3'-hydroxyl and 5'-phosphate groups? 表示 3343 - Why is it difficult to quantify DNA using UV/Vis spectroscopy and fluorescence methods following EpiTect Bisulfite Conversion? How best do you quantify it? FAQ - 3343 表示 3342 - How is the unmethylated unconverted EpiTect DNA control to be used? FAQ - 3342 表示 3315-What is the concentration of the reconstituted QuantiTect Primer Assay (200)? 表示 3322 - What is the concentration of the reconstituted miScript Primer Assay (100) and miScript Precursor Assay (100) tubes? 表示 3317 - Do you have a protocol for isolation of genomic DNA from cell cultures using the QIAamp cador Pathogen Mini Kit? 表示 3318 - What guidelines should I follow in designing primers for use with the EpiTect MSP Kit? 表示 3320 - What is the sensitivity of the Type-it HRM PCR Kit? 表示 3321 - What is the purpose of the C. elegans miR-39 spike-in control in the miRNeasy Serum/Plasma kit? 表示 3318 - What is the preferred amplicon size for performing PCR on DNA amplified with the EpiTect Whole Bisulfitome Kit? 表示 3305 - What is the minimal amount of starting material required for using the SBTexcellerator HLA Kits? 表示表示表示表示 3306 - What is the optimal sample DNA concentration for the Investigator ESSplex SE Plus Kit? 表示 3304 - What is the maximum DNA input volume that can be used in the Investigator ESSplex SE Plus Kit? 表示 3310 - Which settings for the filter set Any5Dye must be adjusted before running the spectral calibration for Investigator ESSplex SE Plus Kit? 表示 3308 - Where can I find the template files for the Investigator ESSplex SE Plus Kit? 表示 3309 - Which filter set should be used with Investigator ESSplex SE Plus Kit? 表示 What additional equipment is needed to purify RNA using the PAXgene 96 Blood RNA Kit? FAQ ID -499 表示 3300 - Can isopropanol or methanol be used in place of ethanol in RNeasy kits? 表示 3301 - Do you have any information or guidelines regarding the choice of reference genes for real-time PCR? 表示 3302 - How long can I store my purified RNA? 表示 3210 - Can the 4-16K Centrifuge be used with a 208 V outlet? 表示 3211 - What is the maximum speed of the plate rotor 2 x 96 in the 4-16 or 4-16K? 表示 3212 - What is the minimum size of DNA fragment that can be isolated with QIAamp DNA Mini kit? 表示 3299-Can Buffer ER from the Endofree Plasmid kits be used instead of the Buffer ETR in QIAGEN Plasmid Plus kits? 表示 3213 - How much ethanol should be added to Buffer AP3/E (renamed to Buffer AW1) and Buffer AW (renamed to Buffer AW2) in the DNeasy Plant kit? 表示 3202 - Can GeneRead rRNA depletion kit be used to deplete rRNA from miRNA isolated using miRNeasy kit? 表示 3200 - What is the Maximum/Minimum amount of RNA that can be used with GeneRead rRNA depletion kit? 表示 3201 - What quality of RNA can be used with the GeneRead rRNA depletion kit? 表示 3204 - How strict is the purification cut-off with GeneRead Size Selection kit? 表示 3203 - Is the RNeasy MinElute cleanup kit required for the GeneRead rRNA depletion kit? 表示 3205 - Can GeneRead Library preparation kits be used on species other than human mouse and rat, for example, plant species and microbial species? 表示 3206 - Can our GeneRead Library preparation kits be used with the SoLiD Platform? 表示 3248 - What is the difference between sponge, cubic and lamellar phase? 表示 3254 - When using the Nextal Cubic Phase products how do I know in which phase the sample is? What will I see? 表示 3255 - When using the NexTal Cubic Phase products, should I work with diluted or undiluted screens? 表示 3227 - In which solution can I keep my protein with the NexTal Cubic Phase kits? 表示 3225 - How pure should my protein be for the NexTal CubicPhase kits? 表示 3217 - How long can I store my purified RNA? 表示 3292-How do I start using the NeXtal CubicPhase kits? 表示 3295-What is the difference between SBTexcellerator HLA Core, Extended and Full Kits? 表示 3240 - What are the main differences between the qBiomarker PCR Arrays and the RT2 Profiler PCR Arrays? 表示 3237 - I would like to order the QuantiFast Probe Assays without master mix. Is this possible? 表示 3233 - Is the yield and quality of RNA and DNA purified with the AllPrep DNA/RNA FFPE Kit comparable to yield and quality of nucleic acids purified separately with other methods. 表示 3265 - Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)? 表示 3298-Will the sequence of the QuantiFast Probe Assays be provided? 表示 3262 - Which thermal cyclers are validated for use with the Investigator Nonaplex ESS Kit? 表示 3263 - Why are samples centrifuged at 4°C after the addition of chloroform when using the RNeasy Plus Universal Mini Kit? 表示 3241 - What are the recommended storage conditions of the QuantiFast Probe RT-PCR Plus Kit components? 表示 3264 - Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA? 表示 3238 - What are important points regarding the genomic DNA wipeout buffer in the QuantiFast Probe RT-PCR Plus Kit? 表示 3245 - What is the concentration of the primers and the probe in a reaction using the QuantiFast Probe Assays? 表示 3239 - What are the differences between the existing QuantiFast Probe RT-PCR Kit and the new Plus Kit? 表示 3247 - What is the correct matrix to use with the Investigator Nonaplex ESS Kit? 表示 3243 - What does the Investigator Nonaplex ESS Kit contain? 表示 3244 -What is the average length of a QuantiFast Probe Assay? 表示 3231 - Is there a size limit to the protein I can crystallize with the NexTal Cubic Phase products? 表示 3228 - Is it also possible to isolate miRNA with the AllPrep DNA/RNA FFPE Kit? 表示 3232 - Is the RNeasy Plus Universal Mini Kit suited for adipose, brain, and other fatty tissues? 表示 3229 - Is it necessary to perform calibration steps for the use of the MAX dye in duplex RT-PCR experiments on different cyclers? 表示 3236 - I use cultured cells as starting material. Can I also use the RNeasy Plus Universal Kit? 詳細を表示 Is there also a miRNeasy Plus Universal Kit available? 表示 3261 - Which purification kits are recommended for the therascreen Pyro kits? 表示 3258 - Which filter set and settings should be used with the Investigator Nonaplex ESS Kit? 表示 3260 - Which purification kits are recommended for the Pyro kits? 表示 3259 - Which probe labels are available for the QuantiFast Probe Assays? 表示 3296-Why does the A peak reduction factor have to de set to 0.86 for the codon 61 assay in the therascreen NRAS Pyro Kit? 表示 What plug-ins are available for the therascreen Pyro kits? FAQ ID - 3251 表示 3249 - What is the optimal DNA volume and concentration for the Investigator Nonaplex ESS Kit? 表示 What plug-ins are available for the Pyrosequencing kits? FAQ ID - 3250 表示 3252 - What should be the concentration of my protein with the NexTal Cubic Phase products? 表示 3256 - Which crystallization solutions are compatible with lipidic cubic phase crystallization? 表示 3253 - What species are QuantiTect Probe Assays offered for? 表示 3257 - Which deparaffinization method can I use with the AllPrep DNA/RNA FFPE Kit? 表示 Can the insertions in Exon 20 be detected with the therascreen EGFR Pyro Kit? 表示 Can the insertions in Exon 20 be detected with the EGFR Pyro Kit? FAQ ID - 3279 表示 3277 - Can I use the RNeasy Plus Universal Mini Kit for all types of tissue? 表示 Can QuantiFast Probe Assays be ordered offline via e-mail or fax? FAQ ID - 3278 表示 3283-Do you have any information or guidelines regarding the choice of reference genes for real-time PCR? 表示 3285-How are the genes in each iPSC qBiomarker PCR Array selected? 表示 3284-For which applications can the RNA isolated with the RNeasy Plus Universal Kit also be used? 表示 Can the QuantiFast Probe Assay detect short fragmented RNA as well as longer fragments of RNA? 表示 Do the RNeasy Plus Universal Mini/Midi kits contain both wash buffers, RW1 and RWT? FAQ ID - 3282 表示 3270 - Are the primer sets different for the Investigator Nonaplex ESS Kit and ESSplex SE where the loci are the same? 表示 Are the QuantiFast Probe Assays hydrolysis or FRET probes? FAQ ID - 3271 表示 Are panels and bin sets available for use with GeneMapper ID and GeneMapper ID-X? FAQ ID - 3267 表示 3275 - Can I use more than 10ng DNA for the PCR reaction for my therascreen Pyro Kits? 表示 Can I use more than 10ng DNA for the PCR reaction for the Pyro kits? FAQ ID - 3276 表示 3274 - Can I do singleplex PCR with the QuantiFast Probe Assays? 表示 3273 - At what temperature can I perform the experiment with the NexTal Cubic Phase kits? 表示 3272 - Are the QuantiFast Probe Assays wet-lab validated? 表示 3222 - How much DNA/RNA can be isolated with the AllPrep DNA/RNA FFPE Kit? 表示 3218 - How many fragments are contained within the size standard BTO in the Investigator Nonaplex ESS Kit? 表示 3219 - How many freeze-thaw cycles can the master mix contained in the QuantiFast Probe RT-PCR Plus Kit tolerate? 表示 3220 - How many PCR cycles should I use for the Investigator Nonaplex ESS Kit? 表示 3226 - How pure should the chloroform used with the RNeasy Plus Universal Mini Kit be? 表示 3223 - How much sample material can I use for DNA/RNA purification with the AllPrep DNA/RNA FFPE Kit? 表示 3224 - How much time can be saved when using the RNeasy Mini QIAcube Kit? 表示 3289-How does the QuantiFast Probe RT-PCR Plus Kit eliminate genomic DNA contamination? 表示 3290-How do I analyze the data for the particular iPSC qBiomarker Arrays I have ordered? 表示 3288-How does the gDNA eliminator solution of the RNeasy Plus Universal Kit work? 表示 3294-How long can I store my purified DNA/RNA isolated with the AllPrep DNA/RNA FFPE kit? 表示 3286-How can I adjust the ROX concentration in the QuantiFast Probe RT-PCR Plus master mix? 表示 3291-How do I harvest the crystals from meso phase? Are special tools needed with the NexTal Cubic Phase products? 表示 3246 - What is the correct matrix to use with Investigator ESSplex SE Plus Kit? 表示 3297-Why does the PCR for HLA-DRB4 result in 2 amplicons of different size? 表示 3266 - Why are two different exon 2 forward sequencing primers used for the SBTexcellerator HLA-DRB1 Kit? 表示 How can I find out if my gene of interest is expressed in a specific tissue type or cell line? FAQ ID - 3214 表示 3215 - What is the starting material for SBTexcellerator HLA Kits? 表示 Can I use genomic DNA from fixed samples with qBiomarker Copy Number PCR Arrays? FAQ ID — 3416 表示 Can I use amplified genomic DNA with qBiomarker Copy Number PCR Arrays? FAQ ID — 3415 表示次ページ Back to top Contact QIAGEN Global contacts Technical Service Customer Care