Frequently Asked Questions - FAQ



FAQ Bookmark シェア more.. You are not authorized to download the resource Sort options アルファベット順関連順 How much protein does one vial of Protease in the QIAamp spin kits contain? What is the concentration of the resuspended Protease? FAQ ID - 3520 表示 What is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue? FAQ ID - 3514 表示 Where can I find additional information for PreAnalytiX PAXgene products? FAQ ID - 3515 表示 How can samples be shipped and stored prior to extraction on the Autopure LS? FAQ ID - 3522 表示 How does the liquid level detection work and what is the minimal detectable volume for the conductive tips of the BioRobot Universal? FAQ ID - 3524 表示 How can samples be shipped and stored prior to extraction on the Autopure LS? FAQ ID - 3528 表示 What is the pipetting throughput of the BioRobot Universal? FAQ ID - 3527 表示 How do I obtain the concentration of my gDNA from the data analysis using the GeneRead DNA QuantiMIZE? FAQ ID - 3530 表示 What is the pipetting throughput of the BioRobot Universal? FAQ ID - 3525 表示 What specification do you have for the high-speed shaker system of the BioRobot Universal? FAQ ID - 3526 表示 Buffer SB1 in the GeneRead Size Selection Kit turned yellowish from a new kit. Can I still use it? FAQ ID - 3562 表示 Is it possible to use the REPLI-g WTA Single Cell kit to amplify miRNA? FAQ ID - 3563 表示 What is the sequence of the index in the GeneRead DNA Adapter I Set 1plex (catalog number 180912)? FAQ ID - 3561 表示 What is the accuracy of the high-speed dispensing system of the BioRobot Universal? FAQ ID - 3523 表示 Can I use the QuantiMIZE system for determination of gDNA quality for non-multiplex PCR enrichment methods (ex. Hybridization) (cat. No. NGQA-002, NGQC-100Y)? FAQ ID - 3531 表示 Which sample materials are CE-IVD validated for the RealStar Filovirus Screen? FAQ ID - 3529 表示 There is no barcode on the QIAsymphony cooling adapter, and the instrument asks for one. What do you suggest I do? FAQ ID - 3532 表示 Can I add bleach to the sample preparation waste? FAQ ID - 3505 表示 What is the recommended range of cells for the miScript Single Cell qPCR Kit? FAQ ID - 3565 表示 Can purified RNA be used with the miScript Single Cell qPCR Kit? FAQ ID - 3566 表示 Can the miScript Single Cell qPCR Kit be used to quantify piRNA expression? FAQ ID - 3567 表示 Can the miScript Single Cell qPCR Kit be used with plant cells? FAQ ID - 3568 表示 Are any of the miScript Single Cell qPCR Kit components interchangeable with miScript II RT Kit, miScript Plant RT Kit, miScript PreAMP PCR Kit, or miScript Microfluidics PreAMP Kit components? FAQ ID - 3569 表示 Should miScript PreAMP Primer Mixes be used in conjunction with the miScript Single Cell qPCR Kit? FAQ ID - 3570 表示 Is the cDNA cleanup step in the miScript Single Cell qPCR Kit workflow absolutely required? FAQ ID - 3571 表示 I have accidentally pipetted beads when removing the eluate after cDNA cleanup. What should I do? FAQ ID - 3572 表示 Does the miScript Single Cell qPCR Kit contain controls? FAQ ID - 3574 表示 When performing real-time PCR quality control associated with the miScript Single Cell qPCR Kit, is it necessary to perform control experiments for all individual samples/cells? FAQ ID - 3576 表示 I do not see any miRNA expression for my miRNAs of interest after performing single cell miRNA expression analysis with the miScript Single Cell qPCR Kit and miScript miRNA PCR Arrays or miScript Primer Assays. What could be the cause? FAQ ID - 3577 表示 What is the amplicon size of PCR products generated with the miScript Single Cell qPCR System? FAQ ID - 3578 表示 What is the dissociation curve temperature of PCR products generated with the miScript Single Cell qPCR System? FAQ ID - 3579 表示 When performing real-time PCR quality control associated with the miScript Single Cell qPCR Kit, is it necessary to perform control experiments for all individual samples/cells? FAQ ID - 3575 表示 Do you recommend normalizing single cell miRNA expression data to a “housekeeping” gene or miRNA? FAQ ID - 3580 表示 How should data be analyzed when the miScript Single Cell qPCR Kit is used with miScript miRNA PCR Arrays or miScript Primer Assays? FAQ ID - 3581 表示 What single cell isolation methods are recommended for use with the miScript Single Cell qPCR Kit? FAQ ID - 3582 表示 How would I provide information on the QuantiTect Primer assays in publications when the sequence is confidential? FAQ ID - 3593 表示 In which tubes is clot detection possible on QIAsymphony? FAQ ID - 3589 表示 How can I create a report/logfile on my QIAsymphony SP system? FAQ ID - 3594 表示 Is it possible to measure the proteolytic activity of proteins on the surface of exosomes that were isolated with ExoEasy? FAQ ID - 3599 表示 Which barcodes on sample tubes are accepted by the QIAsymphony SP? FAQ ID - 3598 表示 Can I change the language on my QIAsymphony SP system? FAQ ID - 3595 表示 Can I use blood, cells or bacteria with the QIAamp Fast DNA Tissue Kit? FAQ ID - 3587 表示 Can the Rotor-Gene Q be connected to LIMS? FAQ ID - 3596 表示 Can the REPLI-g Mitochondrial DNA Kit be used for species other than humans? FAQ ID - 3584 表示 Which Microbial qPCR Mastermix should I use? FAQ ID — 3395 表示 What are the storage conditions for the Microbial DNA qPCR products? FAQ ID — 3394 表示 3327 - How often should the o-ring for the pipettor tip-adapter be changed on the QIAcube? 表示 3326 - Which QIAcube standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet? 表示 3328 - Can the Sample Tubes CB and the Sample Tubes RB be used interchangeably? 表示 3330 - Is there any contamination from E. coli genomic DNA in the polymerase provided with the Repli-G Single Cell Kit? 表示 3329 - When using REPLI-g, if the starting material is a single sorted cell, could mitochondrial DNA (mtDNA) be amplified? 表示 3331 - Should the REPLI-g amplified mtDNA be cleaned up before sequencing? 表示 3332 - Is there a method that can distinguish between non-amplified DNA and REPLI-g amplified DNA? 表示 3333 - When using REPLI-g, what is the purpose of the DTT added to the denaturation buffer in the cells/blood protocol? 表示 What sample types can be tested on the arrays/assays? FAQ ID — 3399 表示 What are the minimum sample requirements for Microbial DNA qPCR kits? FAQ ID — 3393 表示 Can I use the Microbial DNA-Free Water and Microbial qPCR Mastermix if they have been opened more than 3 times? FAQ ID — 3398 表示表示 3353 - What is the composition of the elution buffer in the Ni-NTA Fast Start Kit? FAQ ID - 3353 表示 3352 - What are the size ranges of Ni-NTA particles? FAQ ID - 3352 表示 3354 - What type and amount of resin is packed into the Ni-NTA Spin columns from the Ni-NTA Spin Kit? FAQ ID - 3354 表示 3341 - How are the EpiTect DNA controls produced and tested? FAQ - 3341 表示 3351 - What is the upper limit for protein size that can bind to Ni-NTA agarose resin? FAQ ID - 3351 表示 3344 - What is the difference between the EpiTect Plus DNA Bisulfite kit compared to the classic kit? FAQ - 3344 表示 3345 - What is the difference between the high and low concentration EpiTect Bisulfite Conversion protocol? FAQ - 3345 表示 3311 - Can QuickLyse miniprep kits be used with QIAVac 24 Plus? 表示 3313 - What is the endotoxin level for the QIAGEN Plasmid Plus kits? 表示 3312 - For DirectPrep 96 miniprep, is it possible to use a half plate and save the other half for later prep? 表示 3314-What is the concentration of RNase A sold separately? 表示 3316-What is the concentration of the reconstituted QuantiFast Probe Assay? 表示表示 3325 - What are the catalog numbers for the packing materials for the QIAcube? 表示 3324 - Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube? 表示 3335 - Can I use the DNA directly from enzymatic reaction for Epitect Bisulfite treatment with the Epitect Bisulfite kit (cat. no. 59104)? 表示 3336 - Can I use the linearized DNA for Epitect Bisulfite treatment with the Epitect Bisulfite kit? 表示 3339 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) negatively impact subsequent NGS sequencing? 表示 3337 - Which method is used for QIAGEN sequencing service? 表示 3338 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) have 3'-hydroxyl and 5'-phosphate groups? 表示 3343 - Why is it difficult to quantify DNA using UV/Vis spectroscopy and fluorescence methods following EpiTect Bisulfite Conversion? How best do you quantify it? FAQ - 3343 表示 3342 - How is the unmethylated unconverted EpiTect DNA control to be used? FAQ - 3342 表示 3315-What is the concentration of the reconstituted QuantiTect Primer Assay (200)? 表示 3322 - What is the concentration of the reconstituted miScript Primer Assay (100) and miScript Precursor Assay (100) tubes? 表示 3317 - Do you have a protocol for isolation of genomic DNA from cell cultures using the QIAamp cador Pathogen Mini Kit? 表示 3318 - What guidelines should I follow in designing primers for use with the EpiTect MSP Kit? 表示 3320 - What is the sensitivity of the Type-it HRM PCR Kit? 表示 3321 - What is the purpose of the C. elegans miR-39 spike-in control in the miRNeasy Serum/Plasma kit? 表示 3318 - What is the preferred amplicon size for performing PCR on DNA amplified with the EpiTect Whole Bisulfitome Kit? 表示 3305 - What is the minimal amount of starting material required for using the SBTexcellerator HLA Kits? 表示表示表示表示 3306 - What is the optimal sample DNA concentration for the Investigator ESSplex SE Plus Kit? 表示 3304 - What is the maximum DNA input volume that can be used in the Investigator ESSplex SE Plus Kit? 表示 3310 - Which settings for the filter set Any5Dye must be adjusted before running the spectral calibration for Investigator ESSplex SE Plus Kit? 表示 3308 - Where can I find the template files for the Investigator ESSplex SE Plus Kit? 表示 3309 - Which filter set should be used with Investigator ESSplex SE Plus Kit? 表示 What additional equipment is needed to purify RNA using the PAXgene 96 Blood RNA Kit? FAQ ID -499 表示 3300 - Can isopropanol or methanol be used in place of ethanol in RNeasy kits? 表示 3301 - Do you have any information or guidelines regarding the choice of reference genes for real-time PCR? 表示 3302 - How long can I store my purified RNA? 表示 3210 - Can the 4-16K Centrifuge be used with a 208 V outlet? 表示 3211 - What is the maximum speed of the plate rotor 2 x 96 in the 4-16 or 4-16K? 表示 3212 - What is the minimum size of DNA fragment that can be isolated with QIAamp DNA Mini kit? 表示 3299-Can Buffer ER from the Endofree Plasmid kits be used instead of the Buffer ETR in QIAGEN Plasmid Plus kits? 表示 3213 - How much ethanol should be added to Buffer AP3/E (renamed to Buffer AW1) and Buffer AW (renamed to Buffer AW2) in the DNeasy Plant kit? 表示 3202 - Can GeneRead rRNA depletion kit be used to deplete rRNA from miRNA isolated using miRNeasy kit? 表示 3200 - What is the Maximum/Minimum amount of RNA that can be used with GeneRead rRNA depletion kit? 表示 3201 - What quality of RNA can be used with the GeneRead rRNA depletion kit? 表示 3204 - How strict is the purification cut-off with GeneRead Size Selection kit? 表示 3203 - Is the RNeasy MinElute cleanup kit required for the GeneRead rRNA depletion kit? 表示 3205 - Can GeneRead Library preparation kits be used on species other than human mouse and rat, for example, plant species and microbial species? 表示 3206 - Can our GeneRead Library preparation kits be used with the SoLiD Platform? 表示 3248 - What is the difference between sponge, cubic and lamellar phase? 表示 3254 - When using the Nextal Cubic Phase products how do I know in which phase the sample is? What will I see? 表示 3255 - When using the NexTal Cubic Phase products, should I work with diluted or undiluted screens? 表示 3227 - In which solution can I keep my protein with the NexTal Cubic Phase kits? 表示 3225 - How pure should my protein be for the NexTal CubicPhase kits? 表示 3217 - How long can I store my purified RNA? 表示 3292-How do I start using the NeXtal CubicPhase kits? 表示 3295-What is the difference between SBTexcellerator HLA Core, Extended and Full Kits? 表示 3240 - What are the main differences between the qBiomarker PCR Arrays and the RT2 Profiler PCR Arrays? 表示 3237 - I would like to order the QuantiFast Probe Assays without master mix. Is this possible? 表示 3233 - Is the yield and quality of RNA and DNA purified with the AllPrep DNA/RNA FFPE Kit comparable to yield and quality of nucleic acids purified separately with other methods. 表示 3265 - Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)? 表示 3298-Will the sequence of the QuantiFast Probe Assays be provided? 表示 3262 - Which thermal cyclers are validated for use with the Investigator Nonaplex ESS Kit? 表示 3263 - Why are samples centrifuged at 4°C after the addition of chloroform when using the RNeasy Plus Universal Mini Kit? 表示 3241 - What are the recommended storage conditions of the QuantiFast Probe RT-PCR Plus Kit components? 表示 3264 - Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA? 表示 3238 - What are important points regarding the genomic DNA wipeout buffer in the QuantiFast Probe RT-PCR Plus Kit? 表示 3245 - What is the concentration of the primers and the probe in a reaction using the QuantiFast Probe Assays? 表示 3239 - What are the differences between the existing QuantiFast Probe RT-PCR Kit and the new Plus Kit? 表示 3247 - What is the correct matrix to use with the Investigator Nonaplex ESS Kit? 表示 3243 - What does the Investigator Nonaplex ESS Kit contain? 表示 3244 -What is the average length of a QuantiFast Probe Assay? 表示 3231 - Is there a size limit to the protein I can crystallize with the NexTal Cubic Phase products? 表示 3228 - Is it also possible to isolate miRNA with the AllPrep DNA/RNA FFPE Kit? 表示 3232 - Is the RNeasy Plus Universal Mini Kit suited for adipose, brain, and other fatty tissues? 表示 3229 - Is it necessary to perform calibration steps for the use of the MAX dye in duplex RT-PCR experiments on different cyclers? 表示 3236 - I use cultured cells as starting material. Can I also use the RNeasy Plus Universal Kit? 詳細を表示 Is there also a miRNeasy Plus Universal Kit available? 表示 3261 - Which purification kits are recommended for the therascreen Pyro kits? 表示 3258 - Which filter set and settings should be used with the Investigator Nonaplex ESS Kit? 表示 3260 - Which purification kits are recommended for the Pyro kits? 表示 3259 - Which probe labels are available for the QuantiFast Probe Assays? 表示 3296-Why does the A peak reduction factor have to de set to 0.86 for the codon 61 assay in the therascreen NRAS Pyro Kit? 表示 What plug-ins are available for the therascreen Pyro kits? FAQ ID - 3251 表示 3249 - What is the optimal DNA volume and concentration for the Investigator Nonaplex ESS Kit? 表示 What plug-ins are available for the Pyrosequencing kits? FAQ ID - 3250 表示 3252 - What should be the concentration of my protein with the NexTal Cubic Phase products? 表示次ページ Back to top Contact QIAGEN Global contacts Technical Service Customer Care