FAQ
印刷
Bookmark
シェア
more..
You are not authorized to download the resource
Sort options
アルファベット順
関連順
Why is the initial activation step different for Rotor-Gene Probe, SYBR Green and Multiplex Kits?
FAQ ID -2118
表示
Why is the fluorescence signal of the Internal Control on my Rotor-Gene Q very low?
FAQ ID -2574
表示
Why is the final heat denaturation step so important with the QIAsymphony PAXgene Blood RNA protocol?
FAQ ID -2987
表示
Why is the copy number call lower than expected?
FAQ ID — 3413
表示
Why is the activation time for HotStarTaq Plus Polymerase in the QuantiFast SYBR Green Kits different from that for QuantiFast Probe Kits?
FAQ ID -1449
表示
Why is special Type-it Fast SNP Probe PCR chemistry required for TaqMan® SNP Genotyping?
FAQ ID -2057
表示
Why is my plasmid DNA yield low?
FAQ ID -768
表示
Why is my no template control (NTC) real-time Ct value < 35 cycles in my qPCR Assay?
FAQ ID -2686
表示
Why is my EpiTect Methyl qPCR Assay "failed" as indicated in the QC page of the data analysis Excel file?
FAQ ID -2734
表示
Why is my 260/280 ratio low after using the DNeasy mericon Food Kit?
FAQ ID - 3349
表示
Why is maximum amplicon size for the Type-it Microsatellite PCR Kit limited to 500 bp?
FAQ ID -2060
表示
Why is it recommended to add 1 mM IPTG for optimal protein yields using the EasyXpress Protein Synthesis Kits?
FAQ ID -860
表示
Why is it not recommended to stabilize cells with RNAprotect Tissue Reagent?
FAQ ID -941
表示
Why is EvaGreen instead of SYBR Green used as fluorescent dye in the Type-it HRM PCR Kit?
FAQ ID -2196
表示
Why is DTT used only for semen samples?
FAQ ID -
143761
表示
Why is CoralLoad included in the Type-it Mutation Detect, but not in the Type-it Microsatellite PCR Kit?
FAQ ID -2068
表示
Why is carrier RNA used during the isolation of gDNA from microdissected samples with the QIAamp DNA Micro Kit?
FAQ ID -473
表示
Why is an ice-incubation step included during reaction set-up when following the QuantiTect RT-PCR but not the QuantiTect PCR protocol.
FAQ ID -283
表示
Why is a blood collection set required to collect blood into the PAXgene Blood ccfDNA Tube?
FAQ ID - 3628
表示
Why is a blood collection set required to be used with the PAXgene Blood RNA tube?
FAQ ID - 3459
表示
Why is a 2-step (and not a 3-step) cycling protocol recommended for Rotor-Gene SYBR Green Kits?
FAQ ID -2122
表示
Why is a 2-step (and not a 3-step) cycling protocol recommended for QuantiFast SYBR Green PCR Kits?
FAQ ID -1450
表示
Why is 18S ribosomal RNA (rRNA) used as a housekeeping gene to normalize sample-to-sample, systematic variation in qPCR assays?
FAQ ID -2675
表示
Why have the hazard symbols accompanying products changed?
FAQ ID - 3430
表示
Why had my RT² SYBR Green Mastermix been working well in the past, but now does not seem to be?
FAQ ID -2717
表示
Why does the upper aqueous phase look pinkish when purifying RNA from fatty tissue?
FAQ ID -3118
表示
Why does the template for EasyXpress Disulfide Insect Kits need to have a signal sequence (such as the mellitin signal sequence as provided by the EasyXpress Linear Template Fab Kit primers)?
FAQ ID -2967
表示
Why does the SeqTarget Prep Protocol require a 2-step procedure?
FAQ ID -2238
表示
Why does the QuantiTect Primer Assay for my gene of interest have only one version number?
FAQ ID -1134
表示
Why does the QIAcube sometimes fail to pick up the tips or pick up wrong tips?
FAQ ID -3131
表示
Why does the QIAamp DNA Mini Tissue Protocol require both ATL and AL buffer, while the Blood Protocol only uses AL?
FAQ ID -633
表示
Why does the miScript Target Protector not induce the degradation process?
FAQ ID -2263
表示
Why does the DNeasy mericon Food Kit use a QIAquick column and Buffer PB rather than a DNeasy column and Buffer AL, which might be expected since the kit isolates genomic DNA?
FAQ ID - 3347
表示
Why does the DNeasy mericon Food extraction kit use a QIAquick column and not DNeasy column?
FAQ ID -3148
表示
Why does the copy function not apply relative to migration time and normalized area values after loading the DNA size marker table and adding the alignment marker peaks on the QIAxcel System?
FAQ ID -1832
表示
Why does the connection test fail?
FAQ ID - 141522
表示
Why does the Allprep DNA/RNA 96 Kit use Buffer RLT, whereas the AllPrep DNA/RNA Mini Kit uses Buffer RLT Plus?
FAQ ID -1999
表示
Why does TA/UA cloning work with your proofreading HotStar HiFidelity DNA Polymerase?
FAQ ID -1053
表示
Why does my realtime PCR assay quality decrease over time?
FAQ ID -589
表示
Why does my purified DNA/RNA sometimes have a 260/280 or 260/230 ratio of more than 2? Does that mean the purity is poor?
FAQ ID -3132
表示
Why does my PCR product show up later when comparing the QuantiTect SYBR Green PCR Kits with Roche kits using the same annealing temperature?
FAQ ID -1083
表示
Why does my isolated RNA have a low OD 260/280 ratio?
FAQ ID -97
表示
Why does my DNA sample float out of the slot when loading it onto an agarose gel?
FAQ ID -205
表示
Why does AllPrep DNA/RNA/Protein Mini kit use buffer RLT, but Allprep DNA/RNA Mini and Allprep DNA/RNA Micro kits use buffer RLT Plus? Are these buffers interchangeable?
FAQ ID - 3391
表示
Why do you recommend using Triton X for the purification of 6xHis-tagged protein?
-100
表示
Why do the Internal Control templates for extraction (Internal Control DNA or RNA [High conc.]) have a 10x higher concentration than the IC templates provided with the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2603
表示
Why do replicates in real-time PCR have different plateau heights?
FAQ ID -539
表示
Why do QuantiFast SYBR Green PCR Kits require such a high primer concentration?
FAQ ID -1443
表示
Why do qBiomarker Copy Number PCR Arrays have assays in quadruplicate?
FAQ ID — 3414
表示
Why do pQE-T7 vectors of the QIAgenes E. coli system have an additional UAG amber stop codon?
FAQ ID -2043
表示
Why do my qPCR amplification curves or plots decrease in fluorescence intensity after the saturation phase?
FAQ ID -2689
表示
Why do melting temperatures differ between PCR fragments amplified with QIAGEN's QuantiTect SYBR Green PCR Kits and Roche Kits?
FAQ ID -1084
表示
Why do I sometimes get light blue colonies when using the QIAGEN PCR Cloning Kit?
FAQ ID -603
表示
Why do I see multiple high-intensity peaks in my qPCR dissociation curve at temperatures less than 70 ºC?
FAQ ID -2690
表示
Why do I see low, poor, or sub-standard amplification efficiency in my qRT-PCR assay?
FAQ ID -2695
表示
Why do I observe a CT shift for the Internal Control in some samples in comparison to the CT value for the Internal Control of the dilution series of the Male Control DNA M1?
FAQ ID - 3745
表示
Why do I need to identify my real-time instrument model when placing my order for RT² qPCR Primer Assays?
FAQ ID -2713
表示
Why do I need normalization using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2198
表示
Why do I have wavy DNA bands on my agarose gel?
FAQ ID -2260
表示
Why do I have to use an ACS (Assay Control Set)?
FAQ ID -2933
表示
Why do I have to add beta-mercaptoethanol (beta-ME) to lysis Buffer RLT of the RNeasy Kits?
FAQ ID -101
表示
Why do I get smeared PCR products?
FAQ ID -87
表示
Why do I get genomic DNA contamination in my plasmid prep?
FAQ ID -353
表示
Why do I get amplification in a negative control DNA tube using the REPLI-g Kit for WGA?
FAQ ID -675
表示
Why do casework-sample protocols for the QIAsymphony DNA Investigator Kit start with different lysate volumes (200 µl, 500 µl, or 1000 µl)?
FAQ ID -2035
表示
Why did you choose ORF1a+ORF1b and ORF3a+ORF7a?
FAQ ID - 3893
表示
Why did you choose MAPK1 as a positive control in the QIAGEN RNAi Human/Mouse Control Kit?
FAQ ID -604
表示
Why did we not choose commonly used genes such as the S gene, E gene, or RdRP?
FAQ ID - 3892
表示
Why did the real-time PCR yield Ct values < 12?
FAQ ID -2727
表示
Why can’t I see my QIAcube Connect instrument in the QIAcube Connect App?
FAQ ID - 1414518
表示
Why can't I find the DNA methylation qPCR Assay for my gene of interest?
FAQ ID -2743
表示
Why can I not find the QuantiTect Primer Assay in GeneGlobe that I had previously ordered from QIAGEN?
FAQ ID -1138
表示
Why can I not find the Q-Base device in the device list during the first installation?
FAQ ID - 141516
表示
Why are two reagents used in the PAXgene Tissue System?
FAQ ID -3022
表示
Why are two reagents used in the PAXgene Tissue System?
FAQ ID - 3603
表示
Why are there various different QuantiTect Primer Assays for my gene (previous versions, transcript variants)?
FAQ ID -1133
表示
Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)?
FAQ ID -2366
表示
Why are the RT² qPCR Primer Assays not designed to cross exon-intron junctions or boundaries?
FAQ ID -2710
表示
Why are the QuantiTect and QuantiFast Multiplex PCR Kits limited to triplex real-time PCR on some cyclers?
FAQ ID -715
表示
Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA?
FAQ ID -2365
表示
Why are the denaturation and annealing/extension times for QuantiFast Multiplex RT-PCR Kits much shorter than those for QuantiFast Multiplex PCR Kits?
FAQ ID -2147
表示
Why are the centrifugation speeds for the QIAamp DNA Mini kit at 6000 x g? Can I spin at full speed?
FAQ ID -474
表示
Why are some of my samples outside of the cluster using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2204
表示
Why are some of my RNAprotect-stabilized samples frozen at 0ºC while others are not?
FAQ ID -758
表示
Why are samples centrifuged at 4°C after the addition of chloroform when using RNeasy Lipid Tissue Kits?
FAQ ID -533
表示
Why are samples centrifuged at 4°C after addition of chloroform to the QIAzol lysates in following kits: RNeasy Lipid Tissue Mini/Midi kits, RNeasy Microarray Tissue Mini kit, RNeasy Plus Universal Mini/Midi kit, RNeasy 96 Universal Tissue kit, miRNeasy Mini kit, miRNeasy Micro kit, miRNeasy 96 kit and miRNeasy Serum/Plasma kit?
FAQ ID -2345
表示
Why are not all applications/kits/protocol files visible in the run setup (QIAcube Connect App)?
FAQ ID - 1414520
表示
Why are my realtime PCR amplification plots hook-shaped?
FAQ ID -587
表示
Why are my qPCR Ct values too low (< 12) in my qRT-PCR Assay?
FAQ ID -2684
表示
Why are my qPCR Ct values too high (> 35 or not detectable) in my qRT-PCR assay?
FAQ ID -2685
表示
Why are my DV200 values of my RNA sample so low?
FAQ ID - 3957
表示
Why are most of my samples outside of the cluster in supervised mode using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2205
表示
Who can help in case of issues with the third party instrument?
3799
表示
Who can help if it is not clear if issues are caused by the third party instrument or by the chemistry?
3802
表示
Which version of the EZ2 Connect can run the EZ2 RNA/miRNA Tissue/Cells Kit?
FAQ ID - 3851
表示
Which version of the EZ2 Connect can run the EZ2 RNA FFPE Kit?
FAQ ID - 3940
表示
Which version of the EZ2 Connect can run the EZ2 AllPrep DNA/RNA FFPE Kit?
FAQ ID - 3947
表示
Which variants of the EasyXtal 15-well tool are available?
FAQ ID -2221
表示
Which types and amounts of sample material can be processed for RNA extraction on the QIAsymphony SP?
FAQ ID -1937
表示
Which tubes are recommended for the QIAsymphony sample rack
FAQ ID - 3653
表示
Which transfection reagent is recommended for transfection of the miScript Target Protectors?
FAQ ID -2252
表示
Which software updates are included in a Premium Cover Agreement for my QIAGEN Instrument?
FAQ ID -1336
表示
Which SARS-CoV-2 targets can the SARS-CoV-2 Neo Kit detect?
FAQ ID - 3881
表示
Which sample types can be processed using the QIAsymphony Virus Blood 200 protocol?
FAQ ID -2154
表示
Which sample types can be processed using the QIAsymphony PAXgene Blood RNA Protocol?
FAQ ID -2981
表示
Which sample types can be processed using the PAXgene Blood miRNA protocols?
FAQ ID - 3478
表示
Which sample preparation method is recommended for QIAcuity dPCR?
3779
表示
Which sample materials are CE-IVD validated for the RealStar Filovirus Screen?
FAQ ID - 3529
表示
Which Rotor-Gene Q rotor should be used with the EGFR RGQ PCR kit?
FAQ ID -2315
表示
Which resin is used in the QIAexpress Ni-NTA Fast Start Columns?
FAQ ID -836
表示
Which regions are covered by the GR DNAseq V2 Panels (#181900)?
FAQ ID - 3688
表示
Which real-time PCR kits are recommended downstream of the QuantiTect Whole Transcriptome Kit?
FAQ ID -1592
表示
Which QuantiTect Primer Assay should I choose for my gene of interest?
FAQ ID -1135
表示
Which Qproteome or Protein Fractionation Kits from QIAGEN are compatible with tissue samples?
FAQ ID -755
表示
Which qPCR instrument should I use with your RT² qPCR Primer Assays?
FAQ ID -2714
表示
Which QIAsymphony Virus/Bacteria Kit size should be used for each protocol?
FAQ ID -2038
表示
Which QIAGEN plasmid preparation kits will contain LyseBlue Reagent?
FAQ ID -865
表示
Which QIAGEN kit do you recommend for purifying plasmid DNA suitable for transfection of sensitive cells?
FAQ ID -1092
表示
Which QIAcube Connect standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet?
FAQ ID - 141508
表示
Which PyroMark Gold Q96 Reagent should be used for which instrument and application?
FAQ ID -2836
表示
次ページ
Back to top
Contact QIAGEN
Global contacts
Technical Service
Customer Care
FAQListPage