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What does PAXgene Tissue FIX contain?
FAQ ID -3020
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Can I perform the IDH 1/2 RGQ PCR test with slides containing less than 40% tumor cells?
FAQ ID -3098
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Can the MaXtract High Density Tube be used with QIAzol to isolate RNA?
FAQ ID -1304
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What volume of bacterial culture can be processed using the QuickLyse Miniprep Kit?
FAQ ID -1357
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What factors determine if denatured proteins accumulate underneath, or inside the gel of the MaXtract Low and High Density Tubes?
FAQ ID -1312
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I have a Human Druggable Genome siRNA Set V2.0. Can I reorder the siRNAs from this set?
FAQ ID -1366
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If both MaXtract Low Density and High Density is suitable for an application, which type is preferable to use?
FAQ ID -1310
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How will service work carried out on my QIAGEN BioRobot be documented?
FAQ ID -1331
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If I cannot order FlexiPlate siRNA online, how do I order?
FAQ ID -1371
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What information about my gene or siRNA do I need to order FlexiPlate siRNA?
FAQ ID -1365
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Is it possible to use MaXtract for an organic extraction with a mixture of phenol and BCP (1 bromine chlorpropane)?
-3
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What information is provided with FlexiPlate siRNAs?
FAQ ID -1363
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Is it possible to elute plasmid DNA obtained with the QuickLyse Miniprep Kit in nuclease-free water?
FAQ ID -1355
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Which formats are available for FlexiPlate siRNA?
FAQ ID -1358
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When isolating DNA from plant cells using CTAB, should MaXtract Low or High Density be used?
FAQ ID -1308
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Which parts are exchanged during a Preventive Maintenance visit for my QIAGEN BioRobot?
FAQ ID -1334
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Which controls can I choose for FlexiPlate siRNA?
FAQ ID -1368
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Which density gel type is contained in the yellow or green MaXtract Tubes?
FAQ ID -1315
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What is the composition of FlexiPlate siRNA buffer?
FAQ ID -1361
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Which software updates are included in a Premium Cover Agreement for my QIAGEN Instrument?
FAQ ID -1336
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Can MaXtract tubes be autoclaved prior to use?
FAQ ID -1305
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Can I reduce the amount of starting material below 10 ng when using the REPLI-g UltraFast Mini Kit?
FAQ ID -1325
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Can I reduce the reaction time to e.g., 30 minutes, when using the REPLI-g UltraFast Mini Kit?
FAQ ID -1326
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Can I use any type of DNA extraction method to purify template DNA for amplification using the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1321
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Can several extraction steps be performed in the same MaXtract tube?
FAQ ID -1302
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Can a phenol-chloroform ratio different from 1:1 also be used with MaXtract Low and High Density?
FAQ ID -1309
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Can DNA isolated with MaXtract tubes be used for downstream applications such as restriction digestion, labeling, blotting, PCR, and automated sequencing?
FAQ ID -1303
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Can I amplify mitochondrial DNA directly from whole blood or cells using the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1319
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Can I order Alexa Fluor labeled controls with FlexiPlate siRNA?
FAQ ID -1369
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Can HP Validated siRNAs be ordered on the FlexiPlate?
FAQ ID -1367
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Can frozen sample material pulverized in liquid nitrogen be directly added to MaXtract tubes?
FAQ ID -1301
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Can I also screen large constructs like BACs, fosmids, and cosmids using the QIAGEN PlasmidAmp Kit?
FAQ ID -1337
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How much REPLI-g amplified mitochondrial DNA should be used in a subsequent PCR reaction?
FAQ ID -1320
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Do mutations in the target mitochondrial DNA prevent primer binding, resulting in no DNA amplification with the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1323
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Do you have stability data for genomic DNA isolated with the BioSprint Kits?
FAQ ID -1318
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Do you have stability data for genomic DNA isolated with the Flexigene DNA Kit?
FAQ ID -1317
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Does the addition of sample and organic solution to the MaXtract Tubes require a specific pipetting sequence?
FAQ ID -1307
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How can I upload my gene list for FlexiPlate siRNA to GeneGlobe?
FAQ ID -1364
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How does a Service Support Agreement provide defined budgeting for maintaining my BioRobot?
FAQ ID -1330
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How can I ensure optimal performance of my QIAGEN instrument?
FAQ ID -1329
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Is it possible to purify viral RNA using FastLane Cell RT-PCR Kits?
FAQ ID -1484
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Why is the activation time for HotStarTaq Plus Polymerase in the QuantiFast SYBR Green Kits different from that for QuantiFast Probe Kits?
FAQ ID -1449
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Why is the RT step with the QuantiFast RT Kits much shorter compared to QuantiTect RT Kits?
FAQ ID -1451
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Why is the storage time for QuantiFast PCR Kits shorter than that for QuantiTect PCR Kits?
FAQ ID -1446
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Why is the reaction volume for QuantiFast PCR Kits lower than that for QuantiTect PCR Kits?
FAQ ID -1447
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Why is the QuantiFast denaturation step different for PCR and RT-PCR runs in the two-step protocol for the ABI 7500 and other cyclers?
FAQ ID -1442
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What is the minimum number of siRNAs per order? If I order 40 siRNAs, can I get those on 2 plates?
FAQ ID -1468
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Can purification columns from other suppliers be processed on the QIAcube?
FAQ ID -1406
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Can QIAcube protocol runs be interrupted, and subsequently be continued to completion?
FAQ ID -1414
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Can I use more than 4 µl FastLane lysate in subsequent real-time RT-PCR?
FAQ ID -1480
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Are FlexiPlate siRNAs available at a higher final concentration than 10 µM?
FAQ ID -1476
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Can different amounts of siRNA be ordered on one FlexiPlate?
FAQ ID -1473
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Can 2 µl reaction volumes be used with QuantiFast PCR Kits?
FAQ ID -1440
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Do the QuantiTect Primer Assays work with QuantiFast SYBR Green Kits?
FAQ ID -1439
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Do special settings have to be used for QuantiFast PCR Kits on the Eppendorf Mastercycler ep realplex?
FAQ ID -1437
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What information is required to reorder specific siRNAs from a siRNA Set in FlexiPlate format?
FAQ ID -1463
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What are the turnaround times for FlexiPlate siRNA orders?
FAQ ID -1478
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How much time will be saved when switching from standard cycling to fast cycling with QuantiFast Kits?
FAQ ID -1438
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How many reactions can I perform with the new QuantiFast Kits for real-time PCR?
FAQ ID -1425
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If FlexiPlate siRNA was accidentally redissolved in siRNA Suspension Buffer instead of water, resulting in a 2x buffer, would it affect performance of the siRNAs?
FAQ ID -1477
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How do you achieve fast cycling, yet still deliver the same performance in PCR as that achieved with standard cycling?
FAQ ID -1430
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Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube?
FAQ ID -1415
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Why do QuantiFast SYBR Green PCR Kits require such a high primer concentration?
FAQ ID -1443
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What QuantiFast Kit should be used on the Eppendorf Mastercycler ep realplex?
FAQ ID -1436
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What is the detection limit of the QuantiFast Kits for real-time PCR?
FAQ ID -1432
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Which kind of 96 and 384-well plates are available for FlexiPlate siRNAs?
FAQ ID -1459
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Which cell lines have been tested with FastLane Cell RT-PCR Kits?
FAQ ID -1485
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Can I adjust the ROX concentration in the QuantiFast master mix?
FAQ ID -1427
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Can I follow the reaction setup for probe-based assays described in Appendix D of the FastLane Cell RT-PCR Handbook using 384-well plates?
FAQ ID -1482
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Can FlexiPlate siRNAs be ordered in plates other than the standard NUNC 96 well V bottom plates?
FAQ ID -1458
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Can fast cycling be carried out with QuantiFast Probe PCR Kits and FRET probes?
FAQ ID -1433
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Can FlexiPlate siRNA be ordered in solution on dry-ice?
FAQ ID -1474
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How do QuantiFast PCR Kits compare to QuantiTect PCR Kits for quantitative real-time PCR?
FAQ ID -1441
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How can I get software updates for the QIAcube?
FAQ ID -1413
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Do you offer trial-kit sizes for the new QuantiFast Kits?
FAQ ID -1429
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Can the QIAcube rotor adapters be autoclaved?
FAQ ID -1409
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Can QuantiFast PCR Kits be used on real-time PCR instruments without fast cycling options?
FAQ ID -1428
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Does the high primer concentration required by QuantiFast SYBR Green PCR Kits impair annealing specificity?
FAQ ID -1444
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Does the master mix in the QuantiFast Kits contain dUTP to allow UNG treatments?
FAQ ID -1431
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Can the QuantiFast Probe PCR +ROX Vial mastermix be stored long-term after adding ROX dye?
FAQ ID -1435
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What are important points regarding the genomic DNA wipeout buffer in the QuantiFast Probe RT-PCR Plus Kit?
FAQ ID -2359
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Will the sequence of the QuantiFast Probe Assays be provided?
FAQ ID -2369
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Why are samples centrifuged at 4°C after addition of chloroform to the QIAzol lysates in following kits: RNeasy Lipid Tissue Mini/Midi kits, RNeasy Microarray Tissue Mini kit, RNeasy Plus Universal Mini/Midi kit, RNeasy 96 Universal Tissue kit, miRNeasy Mini kit, miRNeasy Micro kit, miRNeasy 96 kit and miRNeasy Serum/Plasma kit?
FAQ ID -2345
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Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)?
FAQ ID -2366
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Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA?
FAQ ID -2365
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What are the main differences between the qBiomarker PCR Arrays and the RT2 Profiler PCR Arrays?
FAQ ID -2438
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What are the recommended storage conditions of the QuantiFast Probe RT-PCR Plus Kit components?
FAQ ID -2355
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What are the differences between the existing QuantiFast Probe RT-PCR Kit and the new Plus Kit?
FAQ ID -2358
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What is the concentration of the primers and the probe in a reaction using the QuantiFast Probe Assays?
FAQ ID -2370
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What is the average length of a QuantiFast Probe Assay?
FAQ ID -2374
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Is the RNeasy Plus Universal Mini Kit suited for adipose, brain, and other fatty tissues?
FAQ ID -2343
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Is there a size limit to the protein I can crystallize with the NexTal Cubic Phase products?
FAQ ID -2331
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Is the T790M mutation included in the EGFR PCR kit?
FAQ ID -2314
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Is it necessary to perform calibration steps for the use of the MAX dye in duplex RT-PCR experiments on different cyclers?
FAQ ID -2373
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Is there also a miRNeasy Plus Universal Kit available?
FAQ ID -2346
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I use cultured cells as starting material. Can I also use the RNeasy Plus Universal Kit?
FAQ ID -2342
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I would like to order the QuantiFast Probe Assays without master mix. Is this possible?
FAQ ID -2360
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My ADAPT report states "Run Invalid" and does not display any sample results. Why is this?
FAQ ID -2306
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Is V600E the only mutation that can be detected with the codon 600 assay in the BRAF Pyro Kit?
FAQ ID -2382
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Is the yield and quality of RNA and DNA purified with the AllPrep DNA/RNA FFPE Kit comparable to yield and quality of nucleic acids purified separately with other methods.
FAQ ID -2349
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Which purification kits are recommended for the Pyro kits?
FAQ ID -2380
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Which kit, the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, do you recommend for transfection?
FAQ ID -2320
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Which probe labels are available for the QuantiFast Probe Assays?
FAQ ID -2361
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What should be the concentration of my protein with the NexTal Cubic Phase products?
FAQ ID -2325
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What is the maximum plasmid size which I can use with the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit?
FAQ ID -2316
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What plug-ins are available for the Pyrosequencing kits?
FAQ ID -2381
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Which crystallization solutions are compatible with lipidic cubic phase crystallization?
FAQ ID -2418
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Which deparaffinization method can I use with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2352
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What species are QuantiTect Probe Assays offered for?
FAQ ID -2367
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Can the insertions in Exon 20 be detected with the EGFR Pyro Kit?
FAQ ID -2383
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Is it also possible to isolate miRNA with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2348
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Can I use the RNeasy Plus Universal Mini Kit for all types of tissue?
FAQ ID -2338
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Can the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit be used for low-copy number plasmids?
FAQ ID -2317
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Can the QuantiFast Probe Assay detect short fragmented RNA as well as longer fragments of RNA?
FAQ ID -2364
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Can negative crossing threshold (CT) values be negative when using the KRAS and EGFR PCR kits?
FAQ ID -2300
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Can QuantiFast Probe Assays be ordered offline via e-mail or fax?
FAQ ID -2368
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For the the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, can plates be partially used?
FAQ ID -2319
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For which applications can the RNA isolated with the RNeasy Plus Universal Kit also be used?
FAQ ID -2339
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Does the KRAS PCR kit cover the codon 61 mutation?
FAQ ID -2308
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Are the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit suited for short hairpin vectors?
FAQ ID -2318
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Are the QuantiFast Probe Assays hydrolysis or FRET probes?
FAQ ID -2363
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Are the buffers in the RNeasy QIAcube kit delivered in QIAcube buffer bottles?
FAQ ID -2334
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Are QIAshredder spin columns delivered with the RNeasy Mini QIAcube Kit?
FAQ ID -2333
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Can I do singleplex PCR with the QuantiFast Probe Assays?
FAQ ID -2362
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Can I use more than 10ng DNA for the PCR reaction for the Pyro kits?
FAQ ID -2379
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Are the QuantiFast Probe Assays wet-lab validated?
FAQ ID -2375
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At what temperature can I perform the experiment with the NexTal Cubic Phase kits?
FAQ ID -2323
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How much can the mixed standard ?Ct values vary in the KRAS PCR kit?
FAQ ID -2307
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How many samples can be processed per RNeasy Mini QIAcube kit?
FAQ ID -2332
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How much DNA/RNA can be isolated with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2353
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How many freeze-thaw cycles can the master mix contained in the QuantiFast Probe RT-PCR Plus Kit tolerate?
FAQ ID -2357
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How pure should the chloroform used with the RNeasy Plus Universal Mini Kit be?
FAQ ID -2344
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How much sample material can I use for DNA/RNA purification with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2351
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How much time can be saved when using the RNeasy Mini QIAcube Kit?
FAQ ID -2335
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How does the QuantiFast Probe RT-PCR Plus Kit eliminate genomic DNA contamination?
FAQ ID -2354
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How do I analyze the data for the particular iPSC qBiomarker Arrays I have ordered?
FAQ ID -2440
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How are the genes in each iPSC qBiomarker PCR Array selected?
FAQ ID -2439
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How can I adjust the ROX concentration in the QuantiFast Probe RT-PCR Plus master mix?
FAQ ID -2356
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How do I harvest the crystals from meso phase? Are special tools needed with the NexTal Cubic Phase products?
FAQ ID -2330
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What is the advantage of EpiTect MethyLight PCR Kits over QuantiTect Probe PCR Kits for methylation studies?
FAQ ID -2010
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What is the advantage of using the Type-it Microsatellite PCR Kit?
FAQ ID -2059
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What is a good starting amount of template DNA for PCR with the EpiTect MSP Kit?
FAQ ID -2005
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What is the advantage of using the Type-it Mutation Detect PCR Kit?
FAQ ID -2064
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What is the maximum lifetime of the QIAGEN rotor for the Centrifuge 4 and 4K?
-16
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What is the principle behind the DNA Polymerase in the EpiTect MSP Kit?
FAQ ID -2003
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What is the maximum DNA yield from forensic samples processed with the QIAsymphony SP?
FAQ ID -2028
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Is it possible to use different sample types in one run with the MagAttract 96 cador Pathogen Kit?
FAQ ID -2072
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Is magnetic particle carry-over in the eluates on the BioSprint 96 normal?
FAQ ID -2073
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What happens when using EpiTect MethyLight Assays on only partially bisulfite converted DNA template?
FAQ ID -2009
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What are the expected PCR results when using EpiTect Control DNA on untreated or bisulfite-converted DNA?
FAQ ID -2011
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What are the features of the expression vector used for QIAgenes Insect/Mammalia?
FAQ ID -2050
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Why is CoralLoad included in the Type-it Mutation Detect, but not in the Type-it Microsatellite PCR Kit?
FAQ ID -2068
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Why do casework-sample protocols for the QIAsymphony DNA Investigator Kit start with different lysate volumes (200 µl, 500 µl, or 1000 µl)?
FAQ ID -2035
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Why do pQE-T7 vectors of the QIAgenes E. coli system have an additional UAG amber stop codon?
FAQ ID -2043
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Why is the Type-it Mutation Detect PCR Kit recommended for preamplification of SNPs?
FAQ ID -2067
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Why is maximum amplicon size for the Type-it Microsatellite PCR Kit limited to 500 bp?
FAQ ID -2060
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Why is special Type-it Fast SNP Probe PCR chemistry required for TaqMan® SNP Genotyping?
FAQ ID -2057
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Will the optimized DNA sequence for QIAgenes be provided?
FAQ ID -2045
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What sample types can be processed using the QIAsymphony Virus/Bacteria protocols?
FAQ ID -2036
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What sample volumes can be processed with the QIAsymphony Virus/Bacteria Kits?
FAQ ID -2037
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Can data be analyzed on the Rotor-Gene Q while a run is in progress?
FAQ ID -2091
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Are there recommendations for sensitive detection of underrepresented fragments using the Type-it Mutation Detect PCR Kit?
FAQ ID -2066
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Are there two vectors for protein expression using QIAgenes in insect or mammalian cells?
FAQ ID -2048
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Is mitochondrial DNA isolated with the QIAsymphony DNA Investigator Kit on the QIAsymphony SP?
FAQ ID -2030
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Is it possible to edit the sample sheet in the Rotor-Gene Q software after a PCR run?
FAQ ID -2092
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Is it possible to import a standard curve from a previous PCR run on the Rotor-Gene Q?
FAQ ID -2093
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What kind of samples should be processed using the large-volume protocol with the QIAsymphony DNA Investigator Kit?
FAQ ID -2034
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Which QIAsymphony Virus/Bacteria Kit size should be used for each protocol?
FAQ ID -2038
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Which expression systems can be used with the QIAgenes Insect/Mammalia expression constructs?
FAQ ID -2049
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What types of reaction vessels are required for use in the Rotor-Gene Q?
FAQ ID -2085
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Which capillary sequencers can be used for detection of amplicons from the Type-it Microsatellite PCR Kits?
FAQ ID -2063
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Can the EpiTect PCR Control DNAs be used for mouse and rat assays?
FAQ ID -2008
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Can the EpiTect PCR Control DNAs also be used as standard in real-time methylation specific quantification?
FAQ ID -2006
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Can the protocols for purification of small RNAs from cells in the appendices of the AllPrep DNA/RNA 96 Handbook also be used with tissue samples?
FAQ ID -2001
表示
Can QIAGEN rotors used with the centrifuges 4C and 4KC also be used with 4-16 and 4-16K models?
-15
表示
Can the MagAttract 96 cador Pathogen Kit also be used on the KingFisher 96 or MagMAX Express 96 instruments?
FAQ ID -2074
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Can TopTaq Master Mix simply be stored at room temperature on the lab bench for up to 4 months?
FAQ ID -2052
表示
Do all QIAsymphony DNA Investigator protocols use carrier RNA?
FAQ ID -2033
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Can the Type-it Microsaltellite PCR Kit be used on cyclers with fast ramping rates?
FAQ ID -2062
表示
Can the Type-it Mutation Detect PCR kit be used for multiplex PCR of fragments longer than 500 bp?
FAQ ID -2065
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Can 0.1 ml and 0.2 ml tubes used on the Rotor-Gene Q be labeled?
FAQ ID -2082
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Can DNase digestion be performed on the RNeasy 96 plate during the RNA purification part of the Allprep DNA/RNA 96 procedure?
FAQ ID -2000
表示
How many signal readings are taken during the real-time data acquisition on the Rotor-Gene Q?
FAQ ID -2083
表示
Can membrane proteins be expressed with QIAgenes expression kits?
FAQ ID -2047
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Do QIAsymphony Virus/Bacteria samples require pretreatment before they are loaded onto the instrument?
FAQ ID -2039
表示
Does the centrifugal force have any effect on the kinetics of a PCR reaction on the Rotor-Gene Q?
FAQ ID -2081
表示
Does an internal control have to be included for each QIAsymphony Virus/Bacteria protocol run?
FAQ ID -2040
表示
How can full-length genes or individual domains of the QIAgenes Insect/Mammalia constructs be subcloned?
FAQ ID -2051
表示
Can frozen samples be used with the MagAttract 96 cador Pathogen Kit?
FAQ ID -2070
表示
How fast does the rotor spin during a run on the Rotor-Gene Q?
FAQ ID -2080
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How is the log graph threshold value (Ct) for the Rotor-Gene Q determined?
FAQ ID -2088
表示
How long can DNA converted with EpiTect Bisulfite Kits be stored before use in methylation specific PCR with the EpiTect MSP Kit?
FAQ ID -2002
表示
How will running less than 96 samples with the MagAttract 96 cador Pathogen kit affect usage of plasticware?
FAQ ID -2071
表示
If new dyes or chemistries appear on the real-time PCR market, will Rotor-Gene Q be capable of running those dyes?
FAQ ID -2087
表示
If orders cannot be placed online, how can QIAgenes be ordered?
FAQ ID -2046
表示
How much space does the PyroMark Q24 instrument need?
FAQ ID -2098
表示
Does the Type-it Microsatellite PCR Kit always require an annealing temperature of 60°C, or can pre-optimized annealing temperatures for target-primer systems be used?
FAQ ID -2061
表示
What is the turn around time for the ordering of miScript Target Protector?
FAQ ID -2254
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What is the optimal range of PCR fragment sizes for normalization with the SeqTarget System?
FAQ ID -2247
表示
What is the processing time for 96 normalizations with the SeqTarget System?
FAQ ID -2237
表示
What technologies are used by the KRAS and EGFR PCR kits?
FAQ ID -2286
表示
What method do you recommend to quantify DNA before running the KRAS and EGFR PCR kit?
FAQ ID -2292
表示
What kind of automation can be done with the EasyXtal 15-well Tool?
FAQ ID -2220
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What format are the EasyXtal 15-well tools?
FAQ ID -2219
表示
What dyes are used in the KRAS and EGFR PCR kits?
FAQ ID -2288
表示
What do I do if I get amplification in my no template control (NTC) with the KRAS and EGFR PCR kits?
FAQ ID -2298
表示
What do I need to provide when ordering a miScript Target Protector?
FAQ ID -2251
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What is the difference of the sequence of a miScript miRNA Inhibitor and miScript Target Protector?
FAQ ID -2253
表示
What is a miScript Target Protector?
FAQ ID -2249
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What is a miScript Precursor Assay?
FAQ ID -2214
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Which transfection reagent is recommended for transfection of the miScript Target Protectors?
FAQ ID -2252
表示
Which variants of the EasyXtal 15-well tool are available?
FAQ ID -2221
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Why are most of my samples outside of the cluster in supervised mode using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2205
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Why are some of my samples outside of the cluster using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2204
表示
Why does the miScript Target Protector not induce the degradation process?
FAQ ID -2263
表示
Why does the SeqTarget Prep Protocol require a 2-step procedure?
FAQ ID -2238
表示
What will happen, if the normalized DNA for the SeqTarget System is eluted with Buffer EB instead of EB2?
FAQ ID -2242
表示
When negative results are generated using the KRAS and EGFR PCR kits, do I rerun them?
FAQ ID -2294
表示
What type of samples can I use for the KRAS PCR and EGFR kits?
FAQ ID -2293
表示
What voltage should be used to run my agarose gel with the GelPilot Agarose?
FAQ ID -2261
表示
Which mode should I use in the Rotor-Gene ScreenClust HRM Software, supervised or unsupervised?
FAQ ID -2202
表示
Do the KRAS and EGFR PCR kits contain a passive reference dye?
FAQ ID -2295
表示
Does the atypical look of the lyophilized FlexiTube siRNA Premix have a negative impact on performance?
FAQ ID -2267
表示
Does the miScript Target Protector match the target sequence?
FAQ ID -2250
表示
How do I store my FlexiTube siRNA Premix?
FAQ ID -2270
表示
Do I have to order at least 4 FlexiTube siRNA Premixes?
FAQ ID -2266
表示
How is ARMS technology superior to alternative methods such as dye terminator sequencing?
FAQ ID -2290
表示
How do I search for all the miScript Target Protectors for one species?
FAQ ID -2255
表示
How can I check the success of the normalization procedure for the SeqTarget System?
FAQ ID -2245
表示
Can PCR fragments be normalized for the SeqTarget System after purification with QIAquick?
FAQ ID -2240
表示
Are all 3 components of the SeqTarget System required or can a different Long Range PCR Kit and primers be used?
FAQ ID -2234
表示
Can PCR fragments be normalized for the SeqTarget System without a previous purification step?
FAQ ID -2246
表示
Can I use a 384 well plate for the KRAS and EGFR PCR kits?
FAQ ID -2296
表示
Can the miScript Precursor Assay differentiate between pri- and pre-miRNA?
FAQ ID -2211
表示
Does ethanol from the PCR product purification influence the normalization procedure for the SeqTarget System?
FAQ ID -2244
表示
What are EasyXtal 15-well Tools used for?
FAQ ID -2217
表示
What are Principal Components analyzed in Rotor-Gene ScreenClust HRM Software?
FAQ ID -2200
表示
What are the amplicon lengths of the KRAS and EGFR PCR kits?
FAQ ID -2289
表示
What are clusters analyzed in the Rotor-Gene ScreenClust HRM Software?
FAQ ID -2201
表示
Is the SeqTarget System compatible with standard-size PCRs?
FAQ ID -2241
表示
What controls are used in the KRAS and EGFR PCR kits to ensure reliable results?
FAQ ID -2291
表示
What does QIAGEN use for a positive control in precursor detection experiments?
FAQ ID -2212
表示
What do I do if I cannot see amplification in the FAM channel in my KRAS and EGFR PCR kits?
FAQ ID -2297
表示
What are the corresponding QIAGEN names for former Biotage instruments?
FAQ ID -2285
表示
What can be the reason for low DNA yields after normalization with the SeqTarget System?
FAQ ID -2239
表示
How much buffer should be used for agarose gel electrophoresis?
FAQ ID -2257
表示
How long does it take to generate results using the KRAS and EGFR PCR kits?
FAQ ID -2299
表示
How should gels be cast with the GelPilot Agarose so that optimal resolution is achieved?
FAQ ID -2258
表示
How long should PCR fragments be for the SeqTarget System?
FAQ ID -2236
表示
Is it possible to use lower siRNA concentrations with FlexiTube siRNA Premix?
FAQ ID -2264
表示
How many miScript Precursor Assays do you offer?
FAQ ID -2213
表示
Is one able to use FlexiTube siRNA Premix for transfection of primary adherent cell types?
FAQ ID -2268
表示
If I am working with a difficult-to-transfect cell type or if I obtain only weak silencing effects what can I do?
FAQ ID -2265
表示
Is one able to use FlexiTube siRNA Premix for transfection of primary suspension cell types?
FAQ ID -2269
表示
Is it possible to normalize to more or less than 500 ng with the SeqTarget System if needed?
FAQ ID -2235
表示
How many samples can be processed with the QIAsymphony DNA Mini Kit and the Virus Blood 200 protocol?
FAQ ID -2159
表示
How often can the Strep-Tactin Superflow Plus resin be reused?
FAQ ID -2169
表示
Is it possible to purify and normalize long range PCRs produced with PCR kits from other vendors for the SeqTarget System?
FAQ ID -2243
表示
If housekeeping and target genes differ significantly in abundance, will they both be amplified with equal efficiency using the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2129
表示
If Vapor-Lock is used to overlay PCR reactions, which volume should be entered as reaction volume in the Rotor-Gene Q Software?
FAQ ID -2150
表示
Is it possible to purify a GST-tagged protein under denaturing conditions using Glutathione Superflow?
FAQ ID -2172
表示
Is it normal that the binding capacity of GST-resins decreases with flow rate?
FAQ ID -2174
表示
Why is the initial activation step different for Rotor-Gene Probe, SYBR Green and Multiplex Kits?
FAQ ID -2118
表示
Will Rotor-Gene Kits also work on the Rotor-Gene 6000 and 3000 cyclers?
FAQ ID -2121
表示
Why is there an asterisk for my miScript Primer Assays?
FAQ ID -2176
表示
Will Uracil-N-Glycosylase (UNG) completely remove contaminating amplicons when using QuantiTect +UNG Kits?
FAQ ID -2139
表示
Will QuantiTect Primer Assays work with Rotor-Gene SYBR Green Kits using an annealing step at 60ºC?
FAQ ID -2124
表示
What is miRNA*? Do I need to consider this in my profiling experiment?
FAQ ID -2179
表示
What are the main differences between Rotor-Gene and QuantiTect or QuantiFast PCR Kits?
FAQ ID -2119
表示
What are possible reasons for reduced DNA yields with REPLI-g Kits?
FAQ ID -2148
表示
What is the residuals plot in the Rotor-Gene ScreenClust HRM Software?
FAQ ID -2199
表示
What is the target nucleic acid for the Virus Blood 200 Protocol on the QIAsymphony?
FAQ ID -2158
表示
What is the detection limit of the Rotor-Gene and QuantiFast Multiplex RT-PCR Kits?
FAQ ID -2144
表示
What is the minimum number of assays that can be ordered on QuantiTect Primer Assay Plates?
FAQ ID -2109
表示
What is the maximum number of targets that can be amplified per reaction with the QuantiFast Multiplex RT-PCR Kit?
FAQ ID -2145
表示
How should DNA isolated on the QIAsymphony be stored?
FAQ ID -2165
表示
How should samples be stored before purification using the Virus Blood 200 Protocol on the QIAsymphony?
FAQ ID -2157
表示
How pure should the chloroform used with the RNeasy Microarray Tissue Mini Kit be?
FAQ ID -2193
表示
Why should I use the RNeasy Microarray Tissue Mini Kit when purifying RNA for microarray analysis?
FAQ ID -2188
表示
Which elution volumes can be selected using the QIAsymphony Virus Blood 200 protocol?
FAQ ID -2160
表示
What sample volumes can be used with the Virus Blood 200 Protocol on the QIAsymphony?
FAQ ID -2156
表示
Which is the best condition for eluting GST-tagged proteins from Glutathione Superflow?
FAQ ID -2173
表示
What species are QuantiTect Primer Assay Plates offered for?
FAQ ID -2110
表示
Which plate formats are offered for QuantiTect Primer Assay Plates?
FAQ ID -2106
表示
Why are the denaturation and annealing/extension times for QuantiFast Multiplex RT-PCR Kits much shorter than those for QuantiFast Multiplex PCR Kits?
FAQ ID -2147
表示
Which sample types can be processed using the QIAsymphony Virus Blood 200 protocol?
FAQ ID -2154
表示
How many reactions can be performed per well of the QuantiTect Primer Assay Plates?
FAQ ID -2107
表示
Can assays for 20 and 100 reactions be ordered on the same QuantiTect Primer Assay Plate?
FAQ ID -2108
表示
Can QuantiTect Primer Assay Plates be ordered offline via e-mail or fax?
FAQ ID -2112
表示
Can Glutathione Superflow resin be reused?
FAQ ID -2171
表示
Can Uracil-N-Glycosylase (UNG) be added to the QuantiTect PCR Master Mix and be stored for later use?
FAQ ID -2138
表示
Can Uracil-N-Glycosylase (UNG) be purchased separately, independent of QuantiTect PCR Kits?
FAQ ID -2134
表示
Are there any changes in the procedure using the Strep-Tactin Superflow Plus resin with the higher capacity over the standard matrix?
FAQ ID -2168
表示
Can Uracil-N-Glycosylase (UNG) be used with a one-step RT-PCR kit, such as the QuantiTect Probe RT-PCR Kit?
FAQ ID -2135
表示
Can the Rotor-Gene Multiplex RT-PCR Kit be used on other cyclers?
FAQ ID -2142
表示
Can RNA isolated with the RNeasy Microarray Tissue Mini Kit also be used in downstream applications other than microarray analysis?
FAQ ID -2191
表示
Are QuantiTect PCR +UNG Kits available in bulk format (e.g., 25 ml master mix)?
FAQ ID -2136
表示
Are Rotor-Gene Kits compatible with reaction setup using the QIAgility instrument?
FAQ ID -2116
表示
How does template quality influence the results with the Type-it HRM PCR Kit?
FAQ ID -2197
表示
Are the layouts for both 96- and 384-well QuantiTect Primer Assay Plates visible when ordering online?
FAQ ID -2111
表示
Do you have protocols for software setup on various PCR platforms using QuantiFast Probe RT-PCR Kits?
FAQ ID -2105
表示
How does magnetic-particle carry-over in QIAsymphony eluates need to be handled?
FAQ ID -2166
表示
How does the RNeasy Microarray Tissue Mini Kit prevent traces of phenol from negatively affecting the reverse-transcription step in microarray experiments?
FAQ ID -2189
表示
How do Rotor-Gene Probe Kits compare with QuantiFast Probe Kits?
FAQ ID -2125
表示
How many elution steps are recommended when using Glutathione Superflow resin?
FAQ ID -2175
表示
Do you have a protocol for Rotor-Gene software setup for the Rotor-Gene SYBR Green PCR and RT-PCR Kits?
FAQ ID -2104
表示
Does an internal control have to be used with the QIAsymphony Virus Blood 200 Protocol?
FAQ ID -2162
表示
Can Vapor-Lock be used with samples processed on the QIAgility?
FAQ ID -2153
表示
Do the master mixes in Rotor-Gene Kits contain dUTP to allow UNG pretreatment?
FAQ ID -2117
表示
Do QuantiTect Primer Assays also work with Rotor-Gene SYBR Green PCR Kits?
FAQ ID -2123
表示
Do limiting primer concentrations need to be determined when using the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2128
表示
What buffer should be used to dilute cDNA made using the miScript Reverse Transcription Kit?
FAQ ID -1601
表示
Is FlexiTube GeneSolution available for other scales than the 1 nmol scale?
FAQ ID -1666
表示
Is it necessary to clean up cDNA prepared with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1610
表示
Is the master mix of QuantiFast Kits for real-time PCR aliquoted into several tubes to prevent cross-contamination?
FAQ ID -1697
表示
Is the QuantiTect Whole Transcriptome Kit suitable for miRNA amplification?
FAQ ID -1609
表示
Is it necessary to include a genomic DNA removal step with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1614
表示
Is sequence information for HP Validated siRNAs provided in the FlexiTube format?
FAQ ID -1665
表示
Where can I find background information and literature on Whole Genome Amplification with REPLI-g Kits?
FAQ ID -1690
表示
Why is there DNA in the no-template (negative) control when using the QuantiTect Whole Transcriptome Kit?
FAQ ID -1620
表示
What is the maximum volume of RNA in solution that can be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1616
表示
Can the Ni-NTA and Strep-Tactin Superflow Cartridges be reused?
FAQ ID -1607
表示
Can the Ni-NTA Superflow Cartridges and/or Strep-Tactin Cartridges be connected in series?
FAQ ID -1606
表示
Can the QuantiTect Whole Transcriptome Kit be used for amplification of RNA from LCM samples?
FAQ ID -1612
表示
Can I store cDNA generated by the miScript System?
FAQ ID -1602
表示
Does the QuantiTect Whole Transcriptome Kit work with RNA purified from bacteria, yeast, or plants?
FAQ ID -1615
表示
Can T-Script® enzyme of the QuantiTect Whole Transcriptome Kit be substituted by Quantiscript Reverse Transcriptase?
FAQ ID -1617
表示
Can the QuantiTect Whole Transcriptome reaction be stopped after the reverse transcription step to proceed with the ligation and amplification steps at a later time?
FAQ ID -1618
表示
Can I still reorder siRNA that has been removed from the GeneGlobe data base?
FAQ ID -1668
表示
Can amplification with the QuantiTect Whole Transcriptome Kit be extended to more than 8 hours to improve cDNA yields?
FAQ ID -1608
表示
Can I select HP Validated and HP GenomeWide siRNAs for FlexiTube?
FAQ ID -1658
表示
Can cDNA prepared with the QuantiTect Whole Transcriptome Kit be reampliied using REPLI-g Kits?
FAQ ID -1611
表示
Can FlexiTube siRNA or FlexiTube GeneSolution products be ordered in solution?
FAQ ID -1667
表示
How fast is the 6xHis-tagged protein purification process using Ni-NTA Superflow Cartridges?
FAQ ID -1605
表示
How long is cDNA generated with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1613
表示
How long can I archive cDNA generated with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1623
表示
Do I need to use the miScript Universal Primer for detection of mRNA?
FAQ ID -1600
表示
Have you observed co-amplification of genomic DNA from RNA templates used in the QuantiTect Whole Transcriptome Procedure?
FAQ ID -1619
表示
Do you offer a QuantiFast Kit for one-step RT-PCR?
FAQ ID -1695
表示
Do you have a protocol for purification of total RNA from plant latex?
FAQ ID -1694
表示
Do you have a protocol for purification of total RNA from plant tissue with the RNeasy Lipid Tissue Mini Kit?
FAQ ID -1693
表示
What effect does homogenization have on DNA yield and integrity when using AllPrep DNA/RNA Kits?
FAQ ID -1751
表示
Is TopTaq DNA Polymerase a hot-start polymerase?
FAQ ID -1748
表示
What is the fidelity of TopTaq DNA Polymerase?
FAQ ID -1739
表示
Which nucleotide analogs can be used with TopTaq DNA Polymerase?
FAQ ID -1741
表示
Why might Affymetrix GeneChip Mapping assays interfere with the REPLI-g FFPE kit?
FAQ ID -1755
表示
What is the shelf-life of TopTaq DNA Polymerase and Master Mix?
FAQ ID -1743
表示
What is the principle of the REPLI-g FFPE Kit?
FAQ ID -1753
表示
What is the largest plasmid size that can be purified using the QuickLyse Miniprep Kit?
FAQ ID -1752
表示
What is the minimum amount of starting material that can be used with the RNeasy Plus Micro and AllPrep DNA/RNA Micro Kit?
FAQ ID -1750
表示
What is the recommended sample size for use with the REPLI-g FFPE Kit?
FAQ ID -1756
表示
Can small miRNA-containing RNA fractions be separated from large RNAs using the miRNeasy FFPE Kit?
FAQ ID -1737
表示
Can miRNA and other RNAs smaller than 200 nucleotides be isolated with the AllPrep DNA/RNA Micro Kit or RNeasy Plus Micro Kit?
FAQ ID -1749
表示
Can TA/UA cloning be done with PCR products amplified with TopTaq DNA Polmyerase and TopTaq Master Mix Kits?
FAQ ID -1740
表示
Are there problems with the default threshold setting on the Applied Biosystems 7500 when using QuantiFast Probe PCR Kits?
FAQ ID -1702
表示
At which step in the REPLI-g FFPE protocol should PicoGreen quantification be done?
FAQ ID -1758
表示
How can foaming of Buffer RLT Plus lysates be avoided when using RNeasy Plus Micro and Mini Kits, or the Allprep DNA/RNA Mini Kit?
FAQ ID -1734
表示
Do you have protocols for sample processing on the Autopure LS instrument?
FAQ ID -1759
表示
Have QuantiTect Primer Assays been tested with QuantiFast SYBR Green PCR Kits on the Mastercycler ep realplex?
FAQ ID -1714
表示
Can the REPLI-g FFPE procedure restore DNA derived from FFPE tissue samples in the original order?
FAQ ID -1754
表示
Can the REPLI-g FFPE Kit also be used for formalin-fixed, ethanol preserved samples?
FAQ ID -1760
表示
Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the Biorobot M48?
FAQ ID -1733
表示
Does TopTaq Stabilizer of the TopTaq DNA Polymerase and Master Mix Kits interfere with downstream applications?
FAQ ID -1744
表示
Do you have a forensic post-PCR purification protocol to purify double-stranded DNA fragments from PCR reactions?
FAQ ID -1761
表示
Is sample pretreatment required for purifying RNA on the QIAsymphony SP?
FAQ ID -1938
表示
Is the QIAsymphony RNA Kit compatible with stabilized samples?
FAQ ID -1944
表示
Is RNA co-purified with DNA using QIAsymphony DNA Tissue protocols?
FAQ ID -1920
表示
Is there a charge for user training on the QIAsymphony SP System?
FAQ ID -1900
表示
What are the expected DNA yields from tissue processed on the QIAsymphony SP?
FAQ ID -1919
表示
QuantiTect Primer Assays are bioinformatically validated, genomewide primer sets. What does “bioinformatically validated” mean?
FAQ ID -1982
表示
What is the range of elution volumes on the QIAsymphony SP?
FAQ ID -1908
表示
How many samples can be processed per kit or per reagent cartridge for RNA purification on the QIAsymphony SP?
FAQ ID -1940
表示
How does QIAsymphony SP keep cellular samples in suspension prior to pipetting so that samples do not settle?
FAQ ID -1905
表示
How many PCR reactions can be performed for different scales of miScript Primer Assays?
FAQ ID -1990
表示
How efficient is DNA removal by a gDNA Eliminator 96 plate compared with DNase digestion on an RNeasy 96 plate?
FAQ ID -1994
表示
How long can homogenates remain on the QIAsymphony SP without risk of RNA degradation?
FAQ ID -1941
表示
How much sample can be used with the Allprep DNA/RNA 96 Kit?
FAQ ID -1997
表示
Which types and amounts of sample material can be processed for RNA extraction on the QIAsymphony SP?
FAQ ID -1937
表示
How much starting material can be used with the QIAsymphony DNA Mini Kit on the QIAsymphony SP?
FAQ ID -1917
表示
What sample types can be used with the AllPrep DNA/RNA 96 Kit?
FAQ ID -1996
表示
What sample types should be processed with the High Content (HC) protocol for DNA extraction on the QIAsymphony SP?
FAQ ID -1918
表示
What sample types can be used with the RNeasy Plus 96 Kit?
FAQ ID -1991
表示
Will mitochondrial DNA also be isolated using the DNA Tissue protocols on the QIAsymphony SP?
FAQ ID -1921
表示
Why does the Allprep DNA/RNA 96 Kit use Buffer RLT, whereas the AllPrep DNA/RNA Mini Kit uses Buffer RLT Plus?
FAQ ID -1999
表示
Why is there no vacuum-only protocol for the RNeasy Plus 96 Kit?
FAQ ID -1993
表示
What is the turn around time for custom miScript miRNA mimics, inhibitors and primer assays?
FAQ ID -1985
表示
What kind of molecules are miScript miRNA Inhibitors?
FAQ ID -1983
表示
Is it possible to modify QIAsymphony protocols?
FAQ ID -1903
表示
What are the expected yields of total RNA isolated with the QIAsymphony SP?
FAQ ID -1942
表示
What are the starting volumes of homogenate for RNA extraction on the QIAsymphony SP?
FAQ ID -1939
表示
What sample input volumes can be used on the QIAsymphony SP?
FAQ ID -1907
表示
What sample types can be processed using the tissue protocols for DNA extraction on the QIAsymphony SP?
FAQ ID -1915
表示
What kind of molecules are miScript miRNA Mimics?
FAQ ID -1986
表示
What RNA elution volumes are available with the QIAsymphony RNA Kit?
FAQ ID -1943
表示
Do limiting primer concentrations need to be determined when using QuantiFast Multiplex PCR Kits?
FAQ ID -1976
表示
Do samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction?
FAQ ID -1916
表示
Can uneven sample numbers be processed on the QIAsymphony SP module?
FAQ ID -1906
表示
Does HiPerFect work for the transfection of miScript miRNA mimics and inhibitors?
FAQ ID -1984
表示
Does the QIAsymphony SP system generate run reports and/or log files?
FAQ ID -1911
表示
Do you have a protocol for the purification of archive-quality DNA from 100 to 5 x 10e8 cultured cells using Gentra Puregene Kits?
FAQ ID -1960
表示
Do you have a protocol for the purification of archive-quality DNA from 0.5–20 mg paraffin-embedded tissue using Gentra Puregene Kits?
FAQ ID -1964
表示
Do you have a protocol for the purification of archive-quality DNA from 1 ml of whole blood with the Gentra Puregene Tissue Kit?
FAQ ID -1953
表示
Do you have a protocol for rapid purification of archive-quality DNA from up to 9 x 10e6 cells using Gentra Puregene Kits?
FAQ ID -1969
表示
Can I program my own protocols for the QIAsymphony SP?
FAQ ID -1904
表示
Are QuantiFast Multiplex PCR Kits available in trial sizes?
FAQ ID -1981
表示
Can 4-plex, real-time PCR be performed on the ABI PRISM 7000, 7700, or 7900 using the QuantiFast Multiplex PCR Kit?
FAQ ID -1978
表示
Can the QIAsymphony RNA Kit be used to purify RNA from blood?
FAQ ID -1945
表示
Can the QIAsymphony SP Module be connected to a laboratory information management system (LIMS)?
FAQ ID -1909
表示
Can protocol runs on the QIAsymphony SP be interrupted and resumed?
FAQ ID -1912
表示
Can the protocols for purification of small RNAs from cells in the appendices of the RNeasy Plus 96 Handbook also be used with tissue samples?
FAQ ID -1995
表示
Do you have a protocol for the purification of archive-quality DNA from marine invertebrate tissue using Gentra Puregene Kits?
FAQ ID -1950
表示
Do you have a protocol for the purification of archive-quality DNA from nematodes using Gentra Puregene Kits?
FAQ ID -1951
表示
Do you have a protocol for the purification of archive-quality DNA from human dried blood using Gentra Puregene Kits?
FAQ ID -1959
表示
Do you have a protocol for the purification of archive-quality DNA from Gram-negative bacteria using Gentra Puregene Kits?
FAQ ID -1975
表示
Do you have a protocol for the purification of archive-quality DNA from Gram-positive bacteria using Gentra Puregene Kits?
FAQ ID -1946
表示
Do you have a protocol for the purification of archive-quality DNA from hair roots using Gentra Puregene Kits?
FAQ ID -1947
表示
Do you have a protocol for the purification of DNA from fruit flies?
FAQ ID -1970
表示
How can the QIAsymphony SP system be decontaminated?
FAQ ID -1914
表示
Do you have a protocol for the purification of archive-quality DNA from tissue fixed in ethanol or formalin using Gentra Puregene Kits?
FAQ ID -1974
表示
Do you have a protocol for the purification of archive-quality DNA from umbilical cord blood using Gentra Puregene Kits?
FAQ ID -1958
表示
Do you have a protocol for the purification of archive-quality DNA from whole blood using Gentra Puregene Kits?
FAQ ID -1954
表示
Do you have a protocol for the purification of archive-quality DNA from buffy coat using Gentra Puregene Kits?
FAQ ID -1955
表示
Do you have a protocol for the purification of archive-quality DNA from cerebrospinal fluid (CSF) using Gentra Puregene Kits?
FAQ ID -1967
表示
Do you have a protocol for the purification of archive-quality DNA from Chlamydomonas using Gentra Puregene Kits?
FAQ ID -1968
表示
Do you have a protocol for the purification of archive-quality DNA from blood smears using Gentra Puregene Kits?
FAQ ID -1948
表示
Do you have a protocol for the purification of archive-quality DNA from fecal cells using Gentra Puregene Kits?
FAQ ID -1972
表示
Do you have a protocol for the purification of archive-quality DNA from buccal brushes using Gentra Puregene Kits?
FAQ ID -1965
表示
Do you have a protocol for the purification of archive-quality DNA from fish using Gentra Puregene Kits?
FAQ ID -1973
表示
Do you have a protocol for the purification of archive-quality DNA from Eimeria oocysts using Gentra Puregene Kits?
FAQ ID -1952
表示
Do you have a protocol for the purification of archive-quality DNA from fungus using Gentra Puregene Kits?
FAQ ID -1971
表示
Do you have a protocol for cleanup of REPLI-g amplified DNA?
FAQ ID -1545
表示
What are the Tms of PCR products obtained using the miScript System?
FAQ ID -1599
表示
What is Q-Bond used in the QIAGEN Fast Cycling PCR and QuantiFast Kits?
FAQ ID -1554
表示
What controls should be used for normalizing miRNA real-time PCR data obtained with the miScript System?
FAQ ID -1596
表示
Will QuantiTect Primer Assays work at an annealing temperature of 60ºC with QuantiFast SYBR Green PCR and RT-PCR Kits?
FAQ ID -1553
表示
Which homogenization method do you recommend for tissues stored in Allprotect Tissue Reagent?
FAQ ID -1572
表示
Can different incubation times be used for the 2 hour amplification step in the QuantiTect Whole Transcriptome protocol?
-8
表示
Does residual Allprotect Tissue Reagent left behind on tissue samples interfere with DNA, RNA, and protein purification?
FAQ ID -1573
表示
Is cDNA generated with the QuantiTect Whole Transcriptome Kit suitable for use in microarray analysis?
FAQ ID -1587
表示
Do you have a protocol for purification of genomic DNA from cultured cells using the QIAamp DNA Micro Kit?
FAQ ID -1547
表示
Do you have a protocol for concentration of RNA in 96-well plates?
FAQ ID -1546
表示
Do you have a protocol for purification of total RNA from fatty tissues using QIAzol Lysis Reagent and MaXtract High Density?
FAQ ID -1550
表示
Which real-time PCR kits are recommended downstream of the QuantiTect Whole Transcriptome Kit?
FAQ ID -1592
表示
What primers are used in the reverse-transcription step of the QuantiTect Whole Transcriptome procedure?
FAQ ID -1584
表示
Can the AllPrep DNA/RNA/Protein Mini Kit be used with fibrous or lipid tissues?
FAQ ID -1579
表示
What is the maximum amount of RNA that can be used for amplification with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1591
表示
What starting amount of RNA purified using an miRNeasy Kit should be used with the miScript System?
FAQ ID -1597
表示
Can RNA purified from formalin-fixed, paraffin-embedded (FFPE) tissue be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1583
表示
Can samples in Allprotect Tissue Reagent undergo repeated freeze-thaws without degradation of DNA, RNA, and protein?
FAQ ID -1575
表示
Can the QuantiFast Probe PCR +ROX Vial Kit be used on the ABI PRISM 7900 and Applied Biosystems 7500 cyclers?
FAQ ID -1555
表示
Does Buffer APP in the AllPrep DNA/RNA/Protein Mini Kit contain acetone?
FAQ ID -1576
表示
Does cDNA amplified with the QuantiTect Whole Transcriptome Kit correspond to full-length RNA transcripts?
FAQ ID -1585
表示
Can tissue incubated in Allprotect Tissue Reagent be used for morphological studies?
FAQ ID -1571
表示
Can less than 10 ng RNA be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1590
表示
Can cDNA prepared by any method be used as starting material in the ligation reaction of the QuantiTect Whole Transcriptome Kit?
FAQ ID -1589
表示
Can Allprotect Tissue Reagent be used to stabilize RNA, DNA and proteins in cells, bacteria, yeast, and plants?
FAQ ID -1574
表示
Can degraded RNA be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1586
表示
How much cDNA is sufficient for real-time PCR detection of miRNA transcripts generated with the miScript System?
FAQ ID -1598
表示
How many samples can be run in parallel with the Rotor-Gene Q real-time PCR instrument?
FAQ ID -1519
表示
What main input voltage is required for the QIAxcel Analyzer?
FAQ ID -1821
表示
Is there a quality difference between CompactPrep Plasmid Mega and Giga and the CompactPrep Plasmid Midi and Maxi Kits?
FAQ ID -1882
表示
The Gel Cartridge is installed and all status indicators for the QIAxcel System are good but when clicking the "RUN" button, nothing happens. The green highlight goes down the Sequence?
FAQ ID -1850
表示
Why does the copy function not apply relative to migration time and normalized area values after loading the DNA size marker table and adding the alignment marker peaks on the QIAxcel System?
FAQ ID -1832
表示
Can customers use their own DNA size marker with the QIAxcel System and if yes, what concentration should be used?
FAQ ID -1841
表示
Can data be saved at once with the QIAxcel System after analysis of all samples in a 96-well PCR plate?
FAQ ID -1861
表示
How can a positive threshold be set when running the QIAxcel System?
FAQ ID -1843
表示
How long will the intensity calibration procedure on the QIAxcel System take?
FAQ ID -1826
表示
Will the QIAxcel System run using a green Data Review Software Key?
FAQ ID -1853
表示
When should an intensity calibration be re-performed on the QIAxcel System?
FAQ ID -1825
表示
Can the QIAxcel System or QIAxcel Advanced be connected to a network?
FAQ ID -1862
表示
How can a clogged capillary channel be identified when running samples on the QIAxcel System or QIAxcel Advanced?
FAQ ID -1836
表示
Can the QIAxcel software be loaded to another PC to view the data generated with the QIAxcel System?
FAQ ID -1855
表示
Can multiple QIAxcel Systems be run from one computer?
FAQ ID -1860
表示
How often should the solutions in the Buffer Tray for the QIAxcel System or QIAxcel Advanced be replaced?
FAQ ID -1840
表示
How often should the alignment marker for the QIAxcel System be changed?
FAQ ID -1839
表示
If the M500 method is used with the QIAxcel System, does a new DNA reference marker table have to be created?
FAQ ID -1856
表示
In the results table generated with the QIAxcel System, what does "normalized area" mean?
FAQ ID -1854
表示
What is the composition of buffer OCD?
FAQ ID -423
表示
What is the composition of buffer QN?
FAQ ID -414
表示
What is the composition of buffer OCL?
FAQ ID -422
表示
What is the composition of buffer OL2?
FAQ ID -424
表示
Can the QIAamp MinElute Virus Kits be used to extract viral nucleic acid from cell culture supernatant?
FAQ ID -472
表示
Can RNAi experiments be performed in Drosophila?
FAQ ID -436
表示
How is the RNeasy Lipid Tissue Mini Kit different from the RNeasy Mini Kit?
FAQ ID -468
表示
Has RNAi been successful using siRNA in Zebrafish and Xenopus?
FAQ ID -400
表示
Are Northern Blots sensitive enough to detect siRNA-induced gene silencing?
FAQ ID -403
表示
Can I use the RNeasy MinElute Cleanup Kit to clean up my in vitro transcription reaction?
FAQ ID -429
表示
What is the composition of buffer OEB?
FAQ ID -420
表示
Why is the pQE DNA provided in QIAexpress Kits blue in color?
FAQ ID -487
表示
What is the minimum elution volume when using the RNeasy Micro Kit?
FAQ ID -428
表示
What is the maximum amount of starting material that can be processed with the RNeasy Micro Kit?
FAQ ID -426
表示
What is the minimum elution volume when using the RNeasy MinElute Cleanup Kit?
FAQ ID -432
表示
What is the smallest sample size that can be used with RNeasy Mini Kits?
FAQ ID -485
表示
What is a homogeneous LiquiChip assay?
FAQ ID -476
表示
What detection reagents (reporter fluorophores) do you recommend for the LiquiChip System?
FAQ ID -480
表示
What are the excitation and emission wavelengths of the reporter and classification lasers of the LiquiChip Instrument?
FAQ ID -479
表示
What is the binding capacity of the RNeasy MinElute Cleanup Kit column?
FAQ ID -430
表示
What is the composition and size of the LiquiChip Beads?
FAQ ID -475
表示
What is the binding capacity of the RNeasy Micro Kit column?
FAQ ID -427
表示
Is dihydrofolate reductase (DHFR) immunogenic?
FAQ ID -471
表示
What are some of the assays that are adaptable to the LiquiChip System?
FAQ ID -477
表示
My QuantiTect Master Mix did not freeze at degrees. Is it still ok to use?
-20
表示
I want to start with gene silencing (RNAi) experiments. Do you have informational literature?
FAQ ID -580
表示
Is it possible to use less than 1 mg of protein with the PhosphoProtein Purification Kit?
FAQ ID -599
表示
Is it possible to use a QIAzol lysate for the isolation of RNA from tissue on the BioRobot EZ1?
FAQ ID -573
表示
What is a quenched FRET assay?
FAQ ID -542
表示
What do you recommend for isolating RNA from more than 100 mg of plant tissue?
FAQ ID -570
表示
What happens if I spin my lysate on the RNeasy Spin Columns at maximum speed?
FAQ ID -514
表示
How should I dissolve the lyophilized Anti-His and Tag Antibodies?
-100
表示
Is it possible to perform a UNG treatment when using QuantiTect kits?
FAQ ID -564
表示
Is it possible to clean up a methylation reaction containing bisulfite with QIAquick Cleanup Kits?
FAQ ID -519
表示
Is it possible to isolate both RNA and recombinant 6xHis-tagged protein from the same sample?
FAQ ID -532
表示
Why are my realtime PCR amplification plots hook-shaped?
FAQ ID -587
表示
Why are samples centrifuged at 4°C after the addition of chloroform when using RNeasy Lipid Tissue Kits?
FAQ ID -533
表示
How was it determined that the PhosphoSerine Antibody will bind phosphoserines only?
FAQ ID -579
表示
If pQE40 is used to express a fusion protein with DHFR, can the DHFR be cleaved afterwards?
FAQ ID -513
表示
Which chloroform should I use for the RNeasy Lipid Tissue Kit?
FAQ ID -516
表示
Will pancreas tissue swim on top of RNAprotect Tissue Reagent?
FAQ ID -536
表示
Will the 2x QIAGEN Multiplex PCR Master Mix freeze at -20°C?
FAQ ID - 525
表示
Why does my realtime PCR assay quality decrease over time?
FAQ ID -589
表示
What is the longest fragment that was cloned with the pQE UA Cloning Vector?
-30
表示
What is the difference between BSA-free and BSA-containing RGS-His Antibody?
FAQ ID -569
表示
When using QIAzol Lysis Reagent for RNA extraction, I see a pinkish colored aqueous phase! Is it OK?
FAQ ID -534
表示
Where can I find the public Smith-Waterman homology search tool that you refer to on your siRNA online design page?
FAQ ID -562
表示
What reaction volume should I use with QuantiTect Kits?
FAQ ID -551
表示
What makes QIAGEN's 10x Taq and HotStarTaq DNA Polymerase PCR buffer superior?
FAQ ID -566
表示
How many template copies can I detect using QuantiTect Kits in real-time PCR?
FAQ ID -543
表示
Can I use RNAiFect for co-transfection of plasmid DNA and siRNA?
FAQ ID -515
表示
Does DEPC harm RNA?
FAQ ID -529
表示
Does endogenously expressed DAPase in human cells degrade proteins that are expressed in cell culture?
FAQ ID -527
表示
Can the MAPK1 control siRNA be used for rat cells?
FAQ ID -547
表示
Are the columns of the MinElute Reaction Cleanup-, Gel Extraction-, and PCR Purification Kit identical?
FAQ ID -581
表示
After using your U/A PCR Cloning Kit I can detect colonies, which are light blue, and not really white. Why is that?
FAQ ID -512
表示
Can I make a master mix with QuantiProbes, primers, and all other reaction components and store it for later use?
FAQ ID -584
表示
Can I homogenize samples in QIAzol using the QIAshredder?
FAQ ID -501
表示
Can I shorten the activation time for the HotStarTaq DNA Polymerase?
FAQ ID -565
表示
Does QIAGEN sell 6xHis-tagged proteins besides the 6xHis Protein Ladder?
FAQ ID -594
表示
Do you offer QIAshredder spin columns for the RNeasy Midi/Maxi Kit format? If not, what is the alternative?
FAQ ID -560
表示
Do you provide control proteins for use with the PhosphoProtein Purification Kit?
FAQ ID -596
表示
How long should the amplicon be if I am using QuantiTect SYBR Green detection?
FAQ ID -553
表示
How is an antibody epitope determined?
FAQ ID -593
表示
Do I need to calibrate my real-time cycler if I want to use Yakima Yellow?
FAQ ID -540
表示
Do we recommend pooling siRNA sequences?
FAQ ID -517
表示
Does the His-tag interfere with the purification procedure of the QIAGEN PhosphoProtein Purification Kit?
FAQ ID -598
表示
Does the use of MAPK1 as a control in siRNA transfection experiments have secondary effects on the cell culture?
FAQ ID -548
表示
Do you have a protocol to isolate RNA from blood and bone marrow using the BioRobot EZ1?
FAQ ID -567
表示
Do you have any recommendations for using the PosphoProtein Kit with starting material other than cells?
FAQ ID -597
表示
Do you have a protocol for isolating RNA from yeast using RNeasy?
FAQ ID -535
表示
What is the run time for the BioSprint 96 DNA Blood protocol on the BioSprint 96 instrument?
FAQ ID -622
表示
Will REPLI-g work at high temperatures?
FAQ ID -656
表示
The Multiflow Dispense Head on my BioRobot is not dispensing the correct amount of liquid. What can I do?
FAQ ID -652
表示
What is the smallest amount of DNA I can put into a 50 ul REPLI-g reaction?
FAQ ID -663
表示
What is the recommended incubation time to stabilize tissue RNA in RNAprotect Tissue Reagent?
FAQ ID -647
表示
What do you mean by “Quantification of locus representation” in REPLI-g WGA product?
FAQ ID -698
表示
What is REPLI-g whole genome amplification?
FAQ ID -654
表示
What is the average amount of DNA and RNA present in 1 ml normal serum?
FAQ ID -635
表示
What is the biggest protein that can be synthesized with the EasyXpress Protein Synthesis System?
FAQ ID -601
表示
What is the length of the MDA whole genome amplification product?
FAQ ID -690
表示
What is the expected yield of genomic DNA isolated from bacteria with the DNeasy Blood & Tissue or QIAamp DNA Mini Kit?
FAQ ID -632
表示
Is it possible to perform laser microdissection with tissues stabilized with RNAprotect Tissue reagent?
FAQ ID -608
表示
Is it possible to synthesize membrane proteins using the EasyXpress Protein Synthesis Kit?
FAQ ID -602
表示
What are Elution Microtube adapters and Microplate adapters used in the DirectPrep 96 Miniprep Kit, and how can I order them?
FAQ ID -646
表示
What are the advantages of REPLI-g over conventional DNA sample processing and amplification methods?
FAQ ID -686
表示
Why do I get amplification in a negative control DNA tube using the REPLI-g Kit for WGA?
FAQ ID -675
表示
Why did you choose MAPK1 as a positive control in the QIAGEN RNAi Human/Mouse Control Kit?
FAQ ID -604
表示
Will the PhosphoSerine antibody recognize Serine residues if the neighboring residues are Leucine or Isoleucine?
FAQ ID -644
表示
Will TA cloning work with REPLI-g WGA product?
FAQ ID -672
表示
Will DNA from chromosomes collected in TE with a drop of mineral oil work with REPLI-g amplification?
FAQ ID -664
表示
What is the stability of the REPLI-g MDA product?
FAQ ID -693
表示
When delivering liquid to a 96-well plate using the MultiFlow Dispenser Head on my BioRobot , one or more rows in the plate contains less liquid per well than specified by the protocol. What is going wrong?
FAQ ID -653
表示
Where should I add a dye-label modification on the siRNA?
FAQ ID -648
表示
What types of materials from environmental samples could interfere with WGA?
FAQ ID -666
表示
Can I stabilize RNA in bacteria grown on solid media or substrate using the RNAprotect Bacteria Reagent?
FAQ ID -615
表示
Can I re-amplify whole genome amplification product?
FAQ ID -670
表示
Can I use another lysis buffer than the one supplied in the PhophoProtein Purification Kit?
FAQ ID -645
表示
Can I run less than 15 samples on the BioSprint 15 instrument?
FAQ ID -621
表示
Can the QIAshredder Maxi Spin Columns from the DNeasy Plant Maxi Kit be used for RNeasy samples?
FAQ ID -616
表示
Do you have an off-the shelf diagnostics kit for WGA?
FAQ ID -678
表示
Are there components in biological samples that can inhibit the REPLI-g reaction?
FAQ ID -667
表示
Are the BioSprint 15/96 instruments stand-alone workstations?
FAQ ID -620
表示
Can the computer for the LiquiChip Workstation be networked?
FAQ ID -629
表示
Are QIAGEN EZ Competent Cells of the QIAGEN PCR Cloning Kit dam+ or dam-?
FAQ ID -638
表示
Are samples that are contaminated with high molecular weight DNA (e.g. from a paramedic or police officer) a problem with REPLI-g amplification?
FAQ ID -683
表示
Can BAC DNA prepared with the R.E.A.L. Prep 96 Plasmid Kit be used for high-throughput sequencing?
FAQ ID -643
表示
Can I do whole genome amplification from brain tissue using REPLI-g?
FAQ ID -669
表示
Can I do whole genome amplification from mitochondrial DNA using REPLI-g?
FAQ ID -668
表示
Can FTA paper for dried blood spots be used with REPLI-g?
FAQ ID -685
表示
How can I quantify the amount of REPLI-g DNA I have amplified?
FAQ ID -694
表示
Can I amplify from plasma or serum samples using the REPLI-g Mini/Midi kits?
FAQ ID -671
表示
How should I store the REPLI-g amplification product?
FAQ ID -692
表示
Is 2 kb the minimal gDNA fragment size for REPLI-g Whole Genome Amplification?
FAQ ID -682
表示
How long can I store bacterial culture stabilized in RNAprotect Bacteria Reagent?
FAQ ID -614
表示
Do I need to clean up the REPLI-g amplification product for downstream analysis?
FAQ ID -691
表示
Do you have a kit for RNA isolation from any kind of sample type?
FAQ ID -627
表示
Does REPLI-g technology for whole genome amplification work with paraffin embedded samples?
FAQ ID -673
表示
How can I determine the quality of my REPLI-g amplified products?
FAQ ID -695
表示
How can I decrease ribosomal RNA (rRNA) contamination using Oligotex?
FAQ ID -642
表示
Do you know of anything in the buffers/DNA sample that can cause bias in REPLI-g WGA?
FAQ ID -684
表示
Do you have a protocol for the purification of DNA from rodent tail using the BioSprint System?
FAQ ID -979
表示
Do you have a protocol for the isolation of DNA from epithelial cells mixed with sperm cells?
FAQ ID -926
表示
Do you have a protocol for isolation of genomic DNA from saliva using the DNeasy Blood & Tissue Kit?
FAQ ID -931
表示
Do you have a protocol for purification of DNA from clotted blood using the FlexiGene DNA Kit?
FAQ ID -973
表示
Do you have a protocol for Streptokinase treatment of clotted blood?
FAQ ID -912
表示
Do you have a protocol for the isolation of total nucleic acids from animal and human cells on the BioRobot EZ1?
FAQ ID -974
表示
Do you have a protocol for the isolation of total RNA from plant tissue using QIAGEN-tips?
FAQ ID -949
表示
Do you have a protocol for the isolation of total RNA from buccal swabs?
FAQ ID -956
表示
Do you have a protocol for the isolation of viral DNA from stool?
FAQ ID -929
表示
Do you have a protocol for the isolation of total RNA from plants using the RNeasy 96 Kit?
FAQ ID -951
表示
Do you have a protocol for the isolation of DNA from formalin-preserved stool samples?
FAQ ID -922
表示
Do you have a protocol for the isolation of genomic DNA from fungi?
FAQ ID -911
表示
Do you have a protocol for the isolation of genomic DNA and/or proteins from fatty tissue treated with QIAzol?
FAQ ID -955
表示
Do you have a protocol for the purification of DNA from cells using the BioSprint System?
FAQ ID -977
表示
Do you have a protocol for the purification of DNA from dried blood spots using the BioSprint System?
FAQ ID -982
表示
Do you have a protocol for the preparation of tissues preserved in RNAprotect Tissue Reagent for pathological studies?
FAQ ID -950
表示
Do you have a protocol for the purification of DNA from buccal swabs using the BioSprint System?
FAQ ID -983
表示
Do you have a protocol for isolation of genomic and viral DNA from lymphocytes using QIAGEN Genomic-tips?
FAQ ID -901
表示
Do you have a protocol for isolation of genomic and plasmid DNA from cell cultures using QIAGEN Genomic-tips?
FAQ ID -902
表示
Do you have a protocol for transfection of adherent cells with low concentrations of siRNA using RNAiFect Transfection Reagent?
FAQ ID -970
表示
Do you have a protocol for GMO testing of food samples?
FAQ ID -921
表示
Do you have a protocol for isolation of bacterial DNA from soil?
FAQ ID -923
表示
Do you have a protocol for isolation of genomic DNA from dried blood spots using the QIAamp 96 DNA Blood Kit?
FAQ ID -919
表示
Do you have a protocol for isolation of genomic DNA from insects?
FAQ ID -904
表示
Do you have a protocol for detection of HBV DNA by PCR?
FAQ ID -915
表示
Does QIAGEN sell a vector with a T7 promotor for use with the EasyXpress Protein Synthesis Insect Kit?
FAQ ID -937
表示
Can the BioSprint instruments be placed in a cool room?
FAQ ID -938
表示
Do you have a protocol for Acetyl Cysteine (NALC) treatment of viscous samples?
FAQ ID -913
表示
Do you have a protocol for co-transfection of adherent cells with siRNA and plasmid DNA?
FAQ ID -969
表示
Do you have a protocol for adding new reporter dyes to the ABI PRISM 7900 Sequence Detection System?
FAQ ID -965
表示
Do you have a protocol for cleanup of non-radioactive DNA samples?
FAQ ID -945
表示
Do you have a protocol for radioactive labelling of cDNA?
FAQ ID -960
表示
Do you have protocols for multiple extractions of DNA fragments from agarose gels?
FAQ ID -944
表示
Why is it not recommended to stabilize cells with RNAprotect Tissue Reagent?
FAQ ID -941
表示
Do you have a protocol for the detection of Bordetella pertussis DNA by PCR?
FAQ ID -914
表示
Do you have a protocol for the isolation of DNA from cultured cells using the BioRobot EZ1?
FAQ ID -990
表示
Do you have a protocol for performing quantitative multiplex PCR on the LightCycler 2.0 system?
FAQ ID -966
表示
Do you have a protocol for long-term, siRNA-mediated gene silencing using HiPerFect Transfection Reagent?
FAQ ID -972
表示
Do you have a protocol for manual purification of 6xHis-tagged proteins expressed in E. coli using Ni-NTA Superflow?
FAQ ID -967
表示
Do you have a protocol for purification of DNA from cultured animal cells using the DNeasy 96 Blood & Tissue Kit?
FAQ ID -934
表示
Do you have a protocol for purifying 2x96 PCR samples simultaneously with the QIAquick 96 PCR Purification Kit?
FAQ ID -935
表示
Do you have a protocol for purification of DNA fragments from Cy3/Cy5 dye-label reactions?
FAQ ID -947
表示
Do you have a protocol for polyacrylamide gel analysis of oligonucleotides?
FAQ ID -961
表示
Do you have a protocol for isolation of genomic DNA from plants and filamentous fungi using QIAGEN Genomic-tips?
FAQ ID -905
表示
Do you have a protocol for isolation of genomic DNA from flies using QIAGEN Genomic-tips?
FAQ ID -903
表示
What are exo-resistant random hexamers used in the REPLI-g reaction?
FAQ ID -710
表示
The Sigma Centrifuge makes a loud noise at low speeds which disappears at higher speeds, when using the 2x96-well plate rotor. What is the problem?
FAQ ID -776
表示
Is RNAprotect Bacteria Reagent compatible with the RNeasy Mini Kit?
FAQ ID -797
表示
What is the basic technology behind the Strep-tag Protein Purification System?
FAQ ID -740
表示
What are the expected average DNA yields from plant extractions on the BioSprint 96?
FAQ ID -771
表示
If template abundance differs significantly between housekeeping and target gene, are both amplified equally efficient with the QuantiTect Multiplex Kits?
FAQ ID -718
表示
How to quantitate DNA purified using the BioSprint 96?
FAQ ID -773
表示
How to store DNA from forensic samples eluted into water on the EZ1 biorobot in TE buffer instead?
FAQ ID -734
表示
Is it possible to use LiquiChip beads for FACS applications?
FAQ ID -733
表示
Is it possible to buy the EZ Competent Cells of the QIAGEN PCR Cloning Plus Kit separately?
FAQ ID -763
表示
Is GFP excited and detected by the green laser of the LiquiChip System?
FAQ ID -737
表示
Which Qproteome or Protein Fractionation Kits from QIAGEN are compatible with tissue samples?
FAQ ID -755
表示
Why are some of my RNAprotect-stabilized samples frozen at 0ºC while others are not?
FAQ ID -758
表示
Why are the QuantiTect and QuantiFast Multiplex PCR Kits limited to triplex real-time PCR on some cyclers?
FAQ ID -715
表示
Why should DNA or cDNA targets be less than 250 bp long for real-time PCR?
FAQ ID-751
表示
Where can I find info on compatible reporter dyes for use in real-time multiplex PCR using the QuantiTect Multiplex PCR Kits?
FAQ ID -719
表示
Why might my gene of interest drop out during WGA if it is not near a telomere or centromere?
FAQ ID -703
表示
Will the random hexamers in the REPLI-g reaction interfere with downstream analysis?
FAQ ID -713
表示
Why is there no detection of free reporter fluorescence in the LiquiChip System?
FAQ ID -732
表示
What is the cell number range one can use with the FastLane Cell cDNA Kit for real-time RT-PCR?
FAQ ID -782
表示
What negative (unphosphorylated) and positive (phosphorylated) control proteins do you recommend for use with the PhosphoProtein Purification Kit?
FAQ ID -779
表示
What is the enzyme used in the REPLI-g reaction?
FAQ ID -704
表示
What type of Sequencing Services does QIAGEN offer?
FAQ ID -781
表示
Where can I find a copy of the report files generated by running accessory protocols "Dilutor Delivery (monthly)" and "High-Speed Pipetting Sytem (monthly)" on my BioRobot 9604?
FAQ ID -766
表示
What is the general sensitivity level of the LiquiChip System?
FAQ ID -731
表示
Can QIAGEN's Taq- and HotstarTaq DNA Polymerases be used for cycle sequencing?
FAQ ID -741
表示
Can I use the PhosphoProtein Purification Kit to purify phosphorylated peptides of around 1 kD size?
FAQ ID -778
表示
Can RNAprotect Tissue Reagent be used to stabilize tissue which has already been flash-frozen?
FAQ ID -757
表示
Can lyophilized plant material be used for DNA extraction on the BioSprint 96?
FAQ ID -772
表示
Can the REPLI-g WGA enzyme work with labeled dNTPs?
FAQ ID -706
表示
Does the QIAamp DSP Virus Kit require the QIAvac 24 Plus, or can I use it with my own laboratory vacuum system?
FAQ ID -789
表示
Can the MAPK1 siRNA of the RNAi Human/Mouse Starter Kit be purchased separately?
FAQ ID -744
表示
Can the QIAvac 96 vacuum manifold be used with DirectPrep 96 plates?
FAQ ID -722
表示
Are the buffers in the Ni-NTA Fast Start Kit the same as the ones for use with Ni-NTA purchased separately?
FAQ ID -791
表示
Any data on the fidelity of the REPLI-g MDA technique?
FAQ ID -707
表示
Are Centromeres and Telomeres amplified using REPLI-g WGA?
FAQ ID -702
表示
Can I use my own primers for REPLI-g WGA to amplify a specific chromosomal region?
FAQ ID -712
表示
Can I do 4-plex real-time PCR, on the ABI PRISM 7000, 7700, or 7900 using the QuantiTect Multiplex PCR Kit?
FAQ ID -716
表示
How can one perform multiplex real-time PCR analysis using the LightCycler 2.0?
FAQ ID -780
表示
How can I be sure that I am harvesting my induced bacterial culture at the best time point for protein expression?
FAQ ID -788
表示
How do the FastLane Cell cDNA Kit and QuantiTect Reverse Transcription Kit eliminate genomic DNA contamination?
FAQ ID -783
表示
How many cells can be used with the FastLane Cell cDNA Kit?
FAQ ID -796
表示
Do you have an antibody for Western Blot Analysis of MAPK1 gene knockdown using your MAPK1 control siRNA?
FAQ ID -746
表示
Can frozen blood be used for whole genome amplification with REPLI-g?
FAQ ID -794
表示
Do I have to switch off the laser of the LiquiChip Workstation overnight?
FAQ ID -736
表示
How many reporter dyes can be used in the same analysis using the LiquiChip Workstation?
FAQ ID -738
表示
Do I need to determine limiting primer concentrations with the QuantiTect Multiplex PCR Kits?
FAQ ID -714
表示
Do you synthesize thiophosphate modified random hexamers?
FAQ ID -711
表示
Has anyone verified whole genome amplification accuracy with Sequencing?
FAQ ID -701
表示
Do you have a protocol for the fixation of cells transfected with fluorescently labeled siRNA?
FAQ ID -793
表示
How best to handle the light sensitive LiquiChip reagents?
FAQ ID -735
表示
Do you have information on the use of recombinant DNA and RNA as absolute standards for realtime RT-PCR?
FAQ ID -729
表示
Is it possible to transfect DNA into insect cell lines such as Drosophila melanogaster S2?
FAQ ID -397
表示
Is it possible to isolate free genomic DNA using the QIAamp UltraSens Virus Kit?
FAQ ID -358
表示
Is it possible to store TAGzyme enzymes at°C?
-80
表示
I accidentally added RNAprotect Tissue Reagent to my cells, and now the cells are difficult to pellet. What can I do?
FAQ ID -364
表示
What are the calculated molecular weights of the TAGZyme enzymes?
FAQ ID -329
表示
How complete is the removal of DAPase in the TAGZyme process?
FAQ ID -319
表示
How long is fluorescence detectable in cells after transfection with fluorescently labeled siRNAs?
FAQ ID -392
表示
How do I submit a siRNA order by telephone or online?
FAQ ID -399
表示
How many transfections can I perform with 20 nmol of HPP grade siRNA?
FAQ ID -367
表示
Which primers can I use for sequencing pQE-expression vector constructs?
FAQ ID -343
表示
What is the standard assay volume recommended for LiquiChip assays?
FAQ ID -330
表示
What is the acceptable maximum g force for centrifugation of Xa Removal Resin?
FAQ ID -320
表示
What is the lowest elution volume that can be used with QIAprecipitator Midi and Maxi Modules?
FAQ ID -307
表示
What is the composition of Buffer FG3?
FAQ ID -356
表示
Can I use ethanol instead of isopropanol for DNA precipitation when using HiSpeed Plasmid Kits?
FAQ ID -354
表示
303 - Can I use my own lysis buffer with the DNeasy Blood & Tissue or QIAamp DNA Mini Kit?
表示
Can I use a partial MinElute 96 UF plate?
FAQ ID -355
表示
Can I use the RNeasy Mini Kit or RNeasy 96 Kit with fewer than 100 cells?
FAQ ID -372
表示
Can I use water for elution from the QIAprecipitator of the HiSpeed Plasmid Kits?
FAQ ID -306
表示
Are the QIAprecipitator Midi and Maxi Modules of the HiSpeed Plasmid Kits interchangeable?
FAQ ID -308
表示
Apart from the antibody itself, does the Penta-His Biotin Conjugate solution contain any protein?
FAQ ID -359
表示
Are QIAGEN monoclonal Anti His Antibodies glycosylated?
FAQ ID -334
表示
Can I perform in-vitro transcription with the pDrive Cloning Vector from the QIAGEN PCR Cloning Kit?
FAQ ID -332
表示
Do the QuantiTect SYBR Green Kits contain a stabilization reagent that inhibits the sensitivity of real-time PCR?
FAQ ID -328
表示
Do I need to anneal, deprotect or desalt my QIAGEN siRNA?
FAQ ID -398
表示
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