| Cell Line Species/Tissue: |
Human
/
Lung carcinoma |
| Transfection Reagent: |
TransMessenger |
Nucleic Acid:
|
in vitro transcribed RNA |
Growth Medium:
|
DMEM + PSG |
Percent Serum (%):
|
10 % |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
24-well |
Number of Cells:
|
|
Percent Confluence(%):
|
80-90 % |
Amount of Nucleic Acid (µg):
|
0.8 - 1.6 µg |
Amount of Enhancer (µl):
|
1.6 -3.2 µl |
Amount of Reagent (µl):
|
1.6 - 3.2 µl |
Complex Incubation on Cells (hrs):
|
3 h |
Analysis Performed Post-Transfection (hrs):
|
3h |
Transfection Efficiency (%):
|
Not determined |
| Knockdown Efficiency (%): |
|
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Used invitrotranscribed RNA with a Cap (importatnt).
Size of transfected RNA 1300 nt
RNA did encode p53 |
| References: |
| |