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Transfection Cell Database - Detailed View
Cell Line Species/Tissue: |
Human
/
|
Transfection Reagent: |
Attractene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
MEM +FBS, L-glutamine, sodium pyruvate |
Percent Serum (%):
|
10 |
Reporter System:
|
Western Blot anti-FLAG |
Plasmid Purification Method:
|
Endofree Plasmid Kit |
Plate Format:
|
6-well plate |
Number of Cells:
|
5E5/well |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
2 |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
2.25 |
Complex Incubation on Cells (hrs):
|
24 |
Analysis Performed Post-Transfection (hrs):
|
0 |
Transfection Efficiency (%):
|
n/a |
|
Any modifications to the protocol?:
|
No modifications. |
Notes: |
|
References: |
|
|