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Transfection Cell Database - Detailed View


Cell Line: COS-7

Cell Line Species/Tissue: Monkey /  
Transfection Reagent: PolyFect 
Nucleic Acid:
DNA 
Growth Medium:
 
Percent Serum (%):
 
Reporter System:
 
Plasmid Purification Method:
QIAGEN Plasmid Kit 
Plate Format:
6-well plate 
Number of Cells:
3 x 10^5 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
1.5 µg 
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
10 µl 
Complex Incubation on Cells (hrs):
24 hrs 
Analysis Performed Post-Transfection (hrs):
48 hrs 
Transfection Efficiency (%):
45 % 
 
Any modifications to the protocol?:
 
Notes:
It was really easy to use because the manual contains protocols for my cell type (COS-7) and detailed information on the number of cells to seed.  
References: