| Cell Line Species/Tissue: |
Human
/
Lung carcinoma cell |
| Transfection Reagent: |
RNAiFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
DMEM and Ham’s F12 medium |
Percent Serum (%):
|
5% FCS |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
24-well |
Number of Cells:
|
NA |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
1µg |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
6 µl |
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
72 h Western Blot analysis |
Transfection Efficiency (%):
|
NA |
| Knockdown Efficiency (%): |
|
| |
| Any modifications to the protocol?:
|
| none |
| Notes: |
| The cells were either harvested for preparation of whole-cell lysates and Western blot analysis after 72 hours to detect DR5 expression or reseeded in a 96-well plate for additional treatment with CDDO-Me on the second day to detect apoptosis. |
| References: |
| CANCER RESEARCH 64, 7570–7578, October 15, 2004 // Wei Zou et al // c-Jun NH2-Terminal Kinase-Mediated Up-regulation of Death Receptor 5 Contributes to Induction of Apoptosis by the Novel Synthetic Triterpenoid Methyl-2-Cyano-3,12-Dioxooleana-1, 9-Dien-28 |