Find out more about important parameters to assess for sample QC:
Assessing sample quantity and purity
Determining the nucleic acid concentration and purity is a key QC step to ensure success of your downstream experiments. Traditional UV/Vis spectrophotometers cannot differentiate DNA from RNA and only provide quantification of total nucleic acid in your samples. This leaves you in the dark regarding the presence and concentration of other interfering nucleic acids.
Furthermore, nucleic acid samples are often contaminated with other molecules with which they are co-extracted and eluted during purification, and these contaminants can severely impact the success of enzymatic reactions.
By applying smart analysis algorithms for spectral content profiling, QIAxpert lets you differentiate between DNA and RNA and possible sample contaminants. This valuable information lets you make well-informed decisions regarding how to proceed with your samples (see figure RNA spectral profiling analysis).
How the QIAxpert works
How the QIAxpert can help you
Along with the next-generation analysis protocols, QIAxpert users particularly like the clean and simple way of loading samples, the fast measurements and the comprehensive reports that can be automatically exported.
Technical specifications
- Only 2 µl of sample required
- Easy sample loading with capillary intake and storage
- Samples are stable for up to 2 h with no evaporation
- Flexible loading of 1–16 samples
- Unused positions on slide can be used later
- No cross-contamination, no cleaning step
- 0.5 mm optical path; 0.03 to 40 OD (10 mm equivalent)
- DNA range: 1.5–2000 ng/μl dsDNA (A260)