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Bench Guide
PCR

Comprehensive guide to PCR

This section provides a comprehensive guide to PCR. It also includes guidelines and suggestions for maximizing results from your PCR.

Amplification products visualized using CoralLoad PCR Buffer

Introduction

Guidelines for PCR
Types of PCR
PCR primer design
PCR conditions
Guidelines for degenerate primer design and use
- Primer sequence:
- Primer concentration:
Amplification of long PCR products
Enzymes used in PCR
PCR cycling

Commonly used terms in PCR

Commonly used terms in PCR

PCR quantification

PCR quantification
- What is absolute quantification?
- What is relative quantification?
Determining amplification efficiencies

Real time PCR

Real time PCR
- What is SYBR Green I in PCR?
- PCR probes

Real time RT PCR

Guidelines for RT-PCR
DNA contamination
- Removal of DNA contamination
- Detecting DNA contamination in RT-PCR

Digital PCR

Fundamentals of digital PCR
dPCR vs qPCR vs end-point PCR
Comparison of digital PCR methods

Reference Genes and Controls

Endogenous reference genes
PCR controls

References