When developing your own master mix, choosing between different options for Hot Start Taq Polymerases and Reverse Transcriptases can be daunting. From endpoint PCR to multiplex PCR, you can accelerate your assay development process and save time by minimizing trial and error using trusted and proven products from QIAGEN.

In this webinar, let’s break down the key features and differentiators you should look out for when evaluating which DNA Taq Polymerases and Reverse Transcriptases are best suited for your molecular assay.

Got other plans? You can still register and we’ll send you a link to the recorded webinar.

When developing your own master mix, choosing between different options for Hot Start Taq Polymerases and Reverse Transcriptases can be daunting. From endpoint PCR to multiplex PCR, you can accelerate your assay development process and save time by minimizing trial and error using trusted and proven products from QIAGEN.

In this webinar, let’s break down the key features and differentiators you should look out for when evaluating which DNA Taq Polymerases and Reverse Transcriptases are best suited for your molecular assay.

Got other plans? You can still register and we’ll send you a link to the recorded webinar.

Polyomaviruses (PyV) are small viruses with a circular genome. BKPyV and JCPyV are types of polyomaviruses ubiquitous in the human population, and their prevalences increase with age. In healthy individuals, they are not associated with any primary disease but cause persistent infection and can sometimes reactivate.

Reactivation of BKPyV, and less often JCPyV, may occur during immunosuppression during renal transplantation. This may lead to clinically silent nephropathy followed by progressive renal failure, which may be confused with transplant rejection. BKPyV hemorrhagic cystitis is a common outcome of hematopoietic stem cell transplantation. Progressive multifocal leukoencephalopathy (PML) is a rare demyelinating brain infection caused by JCPyV in immunosuppressed patients.

Since there are no standard treatments for these viruses, reducing immunosuppression or restoring immunocompetence are the cornerstones of treatment. To facilitate pre-emptive treatment, quantitative PCR (qPCR) is typically used to monitor PyV load in the urine, blood, or, in suspected PML, cerebrospinal fluid (CSF) samples. The accuracy of qPCR is dependent on the use of assay standards. Therefore, the result includes two-fold variation: 1) that of the sample and 2) that of the standards the sample is compared to.

QIAquity digital PCR (dPCR) is an interesting alternative to qPCR because it’s a standard-free method and is less likely to be affected by possible inhibitory factors. In this webinar, we'll discuss the results of detecting and quantifying JCPyV and BKPyV using QIAquity dPCR in clinical research samples using laboratory designed primers and probes.

Gene therapy holds promise for treating various genetic diseases. In recent years, more than 30 gene therapies have been approved by the FDA, and more than 2000 are currently in development at various pipeline stages around the globe. 

Recombinant Adeno-Associated Viral (AAV) vectors are widely used as a gene delivery mechanism to introduce therapeutic genes in patients. Accurate and reproducible characterization of such vectors is critical to ensure correct clinical dosing, safety, and treatment efficacy.

Genome integrity can influence vector quantitation results depending on the regions targeted for analysis. In addition, truncated vectors may not result in a functional product.

In this roundtable, our experts will discuss the available methods to assess vector integrity and the advantages dPCR-based solutions can bring. They will share perspectives on multiplex assay design to accomplish this task and related challenges, as well as discuss the importance of reference standard materials and assay qualification. 

A live Q&A session will follow the discussion, during which you can ask questions to our expert panelists.

Gene therapy holds promise for treating various genetic diseases. In recent years, more than 30 gene therapies have been approved by the FDA, and more than 2000 are currently in development at various pipeline stages around the globe. 

Recombinant Adeno-Associated Viral (AAV) vectors are widely used as a gene delivery mechanism to introduce therapeutic genes in patients. Accurate and reproducible characterization of such vectors is critical to ensure correct clinical dosing, safety, and treatment efficacy.

Genome integrity can influence vector quantitation results depending on the regions targeted for analysis. In addition, truncated vectors may not result in a functional product.

In this roundtable, our experts will discuss the available methods to assess vector integrity and the advantages dPCR-based solutions can bring. They will share perspectives on multiplex assay design to accomplish this task and related challenges, as well as discuss the importance of reference standard materials and assay qualification. 

A live Q&A session will follow the discussion, during which you can ask questions to our expert panelists.