Optimizing the extraction of human microbiome samples
| The influence of the human microbiome on health is an important area of ongoing investigation for medicine and diagnostics. For these studies, it is crucial to have a reliable, reproducible extraction method which is able to extract DNA of all microorganisms with comparable efficiency, while simultaneously depleting inhibitory substances which might disturb subsequent downstream analysis. We present the results of DNA extraction and microbiome analysis from stool, saliva, and a variety of swabs (buccal, vaginal, rectal, skin) using the DNeasy PowerSoil Pro/QIAamp PowerFecal Pro kits, including automation options and process handling improvements. The DNeasy PowerSoil Pro and QIAamp PowerFecal Pro kits are an optimal method for extracting microbial DNA from human samples for microbiome analysis and can be used for a wide variety of sample types in addition to stool. |
About the speaker
Dominic O’Neil, Senior Scientist
QIAGEN
Dominic O’Neil has over 13 years of experience in the biotechnology industry. Before joining QIAGEN, he gained molecular biology expertise at several companies, including three years at the Whitehead/MIT Center for Genome Research in Cambridge, MA, where he participated in the completion of the initial draft of the human genome. Dominic joined Digene (which later became part of QIAGEN) in 2004 to work on new technology research and development, focusing in particular on sample preparation and diagnostic applications. In 2011, he joined the QIAGEN R&D group in Hilden as a Senior Scientist to work on solutions for next-generation sequencing. He is now the Director R&D for Microbiome Product Development.
Categories
Microbiome