Take the shortcut – library normalization without quantification
You’ve prepared your NGS libraries and are eager to get started with sequencing right away. But wait, don’t you have to quantify your libraries first? Save time and ditch that traditional time-consuming library quantification method. With our QIAseq Normalizer Kit, you can easily pool libraries without the need for library quantification and still achieve qPCR-level accuracy. All it takes is 30 min. The specially developed, fast and cost-effective workflow lets you reliably normalize libraries across a wide range of concentrations. This means you can effortlessly achieve consistent and high-quality data and avoid issues like uneven representation of libraries.
Top 5 reasons to choose the QIAseq Normalizer Kit over traditional library quantification methods:
- Normalized, ready-to-sequence libraries at 4 nmol/L
- Streamlined, 30-min benchtop protocol
- Gold-standard quality of qPCR without the long workflow
- Works with a broad range of concentrations from 15 to over 300 nmol/L
- Compatible with various types of libraries (whole genome, whole transcriptome and target-enriched libraries)
"QIAseq Library Normalizer Kit is fast, easy to use and easy to integrate into the library preparation workflow and outperformed the fluorimetry method without the need for library quantification."
Dr. Christine Haag, Senior Scientist, Department of Molecular Endocrinology, MVZ lab, Heidelberg, Germany
See why normalization matters
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