Add internal markers or label at the ends
There are two ways to label DNA with enzymes: at the ends or internally along the molecule. Detectable markers such as fluorophores or modified nucleotides can be introduced by the addition of 5’ and 3’ groups through enzyme action, as well as by introducing internal markers through direct incorporation or post-labeling techniques with a suitable enzyme.
Strategies for labeling
Enzyme strategies for adding labels to nucleic acids
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Labeling by the action of an enzyme is required for many studies aiming to elucidate the functions and dynamics of nucleic acids in vitro and in cells. For every site where labeled nucleotides can be added to DNA or RNA, there is a suitable enzyme that can catalyze the addition.
Enzymes for labeling
Discover featured enzymes for labeling nucleic acids
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FAQs about labeling nucleic acids
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