Small-scale plasmid purification — 96-well format
| Kit name | QIAGEN Plasmid Plus 96 Miniprep and BioRobot Kits |
QIAprep 96 Plus Miniprep and BioRobot Kits |
QIAprep 96 Turbo Miniprep and BioRobot Kits |
|---|---|---|---|
| Speed | Standard | Fast | Fast |
| DNA grade |
Transfection grade |
Transfection grade |
Molecular biology grade |
| Binding capacity |
<50 µg | <50 µg | <20 µg |
| Culture volume (3–4 x 109 cells/ml) |
1.25 –5 ml | 1.25 –5 ml | 1–5 ml |
| Yield | 15–50 µg | 15–50 µg | 4–20 µg |
| Elution volume |
80 µl (100–120 µl for a 5 ml culture) |
80 µl (100–120 µl for a 5 ml culture) |
100 µl |
| Procedure | QIAvac 96, centrifuge, BioRobot Universal System |
QIAvac 96, centrifuge, BioRobot Universal System |
QIAvac 96, centrifuge, BioRobot Universal System |
| Time | 50 min | 40 min | 45 min |
Small-scale plasmid purification kits
| Kit name | QIAGEN Plasmid Mini Kit |
QIAprep Spin Miniprep Kit |
Quicklyse Miniprep Kit |
|---|---|---|---|
| Speed | Fast | Fast | Ultrafast |
| DNA grade | Transfection grade |
Molecular biology grade |
Sequencing grade |
| Binding capacity |
<20 µg | <50 µg | <15 µg |
| Culture volume (3–4 x 109 cells/ml) |
3 ml | 1–5 ml | 1–3 ml |
| Yield | 20 µg | 5–50 µg | 8–15 µg |
| Elution volume | Variable, 50 µl recommended |
50 µl | 50 µl |
| Procedure | Centrifuge, QIAcube Connect |
QIAvac 24 Plus, centrifuge, QIAcube Connect |
Centrifuge |
| Time | 80 min | 30 min | 9 min |
Large-scale plasmid purification kits
| Kit name | EndoFree Plasmid Kits |
QIAGEN Plasmid Kits |
QIAfilter Plasmid Kits |
QIAGEN Plasmid Plus Kits |
HiSpeed Plasmid Kits |
QIAGEN Large-Construct Kit |
CompactPrep Plasmid Kits |
|
|---|---|---|---|---|---|---|---|---|
| Speed | Standard | Standard | Fast |
Ultrafast | Ultrafast | Standard | Ultrafast | |
| DNA grade | Endotoxin-free | Transfection grade |
Transfection grade |
Transfection grade |
Transfection grade |
Molecular biology grade |
Molecular biology grade |
|
| Culture volume (3–4 x 109 cells/ml) |
Midi | _ |
25 ml, 100 ml |
25 ml, 50–100 ml |
20–25 ml, 25–35 ml (hy), 50 ml |
50 ml, 150 ml |
500 ml | 25 ml, 50 ml |
| Maxi | 100 ml, 250 ml |
100 ml, 500 ml |
100 ml, 250 ml |
80–100 ml, 100–130 ml (hy), 200 ml |
150 ml, 250 ml |
100 ml, 250 ml |
||
| Mega | 500 ml/1.5 g, 2.5 l / 7.5g |
500 ml/1.5 g, 2.5 l/ 7.5g |
500 ml/1.5 g, 2.5 l/7.5g |
500 ml |
500 ml only rec for hc |
– | ||
| Giga | 2.5 l/7.5 g, not rec. for lc |
2.5 l/7.5 g, 5 l/15 g |
2.5 l/7.5 g, not rec. for lc |
2.5 l |
2.5 l only rec for hc |
– | ||
| Kit name | EndoFree Plasmid Kits |
QIAGEN Plasmid Kits |
QIAfilter Plasmid Kits |
QIAGEN Plasmid Plus Kits |
HiSpeed Plasmid Kits |
QIAGEN Large-Construct Kit |
CompactPrep Plasmid Kits |
|
|---|---|---|---|---|---|---|---|---|
| Yield | Midi | – |
75–100 µg, 20–100 µg |
75–100 µg, 20–100 µg |
100–200 µg, 150–250 µg (hy), 30–100 µg |
100–200 µg, 30–100 µg |
Up to 50 µg BAC/PAC/P1 DNA, up to 200 µg cosmid DNA |
100–200 µg, 30–100 µg |
| Maxi | 300–500 µg, 50–250 µg |
100–500 µg, 20–250 µg |
300–500 µg, 50–250 µg |
750 µg, 1 mg (hy), 50–250 µg |
300–750 µg, 50–250 µg |
350–750 µg, 50–250 µg |
||
| Mega | 1.5–2.5 mg, 0.5–2.5 mg |
1.5–2.5 mg, 0.5–2.5 mg |
1.5–2.5 mg, 0.5–2.5 mg |
Up to 2.5 mg |
1.5–2.5 mg, endotoxin-free <0.1 EU/µg DNA |
– | ||
| Giga | 7.5–10 mg |
7.5–10 mg, 1–5 mg |
7.5–10 mg |
Up to 10 mg |
7–10 mg, endotoxin-free <0.1 EU/µg DNA |
– | ||
| Time | Midi | – |
150 min |
110 min |
20 min |
<45 min |
280 min | <20 min |
| Maxi | 150 min |
160 min |
120 min |
20 min |
<60 min |
<20 min | ||
| Mega | 220 min |
220 min |
190 min |
40 min |
50 min |
– | ||
| Giga | 310 min |
320 min |
280 min |
50 min |
50 min |
– | ||
| Procedure | Lysate clearing by precipitator or vacuum, gravity-flow |
Gravity-flow | Lysate clearing by precipitator or vacuum, gravity-flow |
Vacuum and centrifuge |
Gravity-flow and precipitator |
Centrifuge | Centrifuge and vacuum |
|
For up-to-date licensing information and product-specific disclaimers, see the respective QIAGEN kit instructions for use or user operator manual. QIAGEN instructions for use and user manuals are available at www.qiagen.com or can be requested from QIAGEN Technical Services (or your local distributor).