常见问题

 

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Do the samples require pretreatment before starting the manual protocol or before loading onto the QIAcube instrument?
FAQ ID - 3479
What are the expected yields of RNA from PAXgene Blood RNA Tubes isolated with the PAXgene Blood miRNA kit?
FAQ ID - 3481
Do you have stability data for RNA isolated with PAXgene miRNA Kit?
FAQ ID - 3480
Can the PAXgene Blood DNA Tubes be stored at 4°C?
FAQ ID - 3493
How should I thaw frozen PAXgene Blood DNA Tubes?
FAQ ID - 3495
Can the PAXgene Blood DNA Tube be frozen? If yes, at what temperatures and for how long?
FAQ ID - 3491
Can mitochondrial DNA be isolated with the PAXgene Blood DNA System?
FAQ ID - 3498
3497 - What is the expected purity and size of DNA extracted using the PAXgene Blood DNA kit?
FAQ ID - 3497
Does the DNA extracted using the PAXgene Blood DNA System contain RNA?
FAQ ID - 3499
Can I multiplex the Microbial DNA qPCR Assays?
FAQ ID — 3408
Can I measure virulence factor gene expression?
FAQ ID — 3405
What sample types can I test on qBiomarker Copy Number PCR Arrays?
FAQ ID — 3423
What is the sensitivity for the Microbial DNA qPCR kits?
FAQ ID — 3409
Which probe labels are available for the Microbial DNA qPCR Assays?
FAQ ID — 3407
Are the Microbial DNA qPCR Assays wet-lab verified?
FAQ ID — 3406
Can I measure antibiotic resistance gene expression?
FAQ ID — 3404
What sequences are used to design the Microbial DNA qPCR Assays?
FAQ ID — 3403
Are the assays species-specific?
FAQ ID — 3400
Why is the copy number call lower than expected?
FAQ ID — 3413
Will qBiomarker Copy Number Assays work with heterogeneous samples like mixtures of tumor and normal tissue?
FAQ ID — 3419
Why do qBiomarker Copy Number PCR Arrays have assays in quadruplicate?
FAQ ID — 3414
How should I analyze the data generated from a qBiomarker Copy Number PCR Array experiment?
FAQ ID — 3421
What could have gone wrong if the CT values are unusually high for all wells in a sample?
FAQ ID — 3412
What qPCR mastermix should I use with the qBiomarker Copy Number PCR Arrays and Assays
FAQ ID — 3422
How much DNA should I use on a qBiomarker Copy Number PCR Array?
FAQ ID — 3417
How much DNA should I use in a qBiomarker Copy Number PCR Assay?
FAQ ID — 3418
How do you choose which assays to include on the arrays?
FAQ ID — 3420
What testing should be performed in order to assess the quality of a DNA sample?
FAQ ID — 3424
How many samples can be processed with the PAXgene Blood miRNA Kit?
FAQ ID - 3477
Why have the hazard symbols accompanying products changed?
FAQ ID - 3430
Why is a blood collection set required to be used with the PAXgene Blood RNA tube?
FAQ ID - 3459
As part of my RT procedure I routinely heat an aliquot of the eluate prior to the RT reaction. Do I still need to heat the whole eluate after the RNA preparation?
FAQ ID - 3468
Is there any chance of the PAXgene Blood RNA tube reagent going back into the patient's arm?
FAQ ID - 3460
I need to buy a new computer for my PyroMark Q96. Can I reinstall the PyroMark Q96 software on my new computer or do I need to purchase a new license?
FAQ ID - 3472
Can I reinstall the PyroMark Q24 software on my new computer or following an operating system upgrade, or do I need to purchase a new license?

FAQ ID - 3473

Can the PAXgene Blood DNA Tubes be stored at higher temperatures?
FAQ ID - 3494
What are the expected yields of RNA from PAXgene Blood RNA Tubes using the QIAsymphony PAXgene Blood RNA Kit?
FAQ ID - 3475
What additional equipment is needed to purify RNA using the PAXgene 96 Blood RNA Kit?
FAQ ID - 3474
Is it possible to isolate genomic DNA or viral nucleic acids with the QIAsymphony PAXgene Blood RNA protocol?
FAQ ID - 3476
2. Are there any special considerations for the collection of blood using PAXgene Blood DNA Tubes CE (e.g., use of a blood collection set or discard tube, positioning of the tube, issues with short draws, etc.)?
FAQ ID - 3485
Are the PAXgene Blood DNA Tubes sterile?
FAQ ID - 3487
What is the additive in PAXgene Blood DNA Tubes
FAQ ID - 3486
What is the maximum number of samples for the QIAcube variant of the PAXgene Blood miRNA protocol for a single run?
FAQ ID - 3482
Do I have to use a blood collection set with the PAXgene Blood DNA Tube?
FAQ ID - 3484
Is it possible to isolate genomic DNA or viral nucleic acids with PAXgene Blood miRNA protocol?
FAQ ID - 3483
How should blood samples drawn into PAXgene Blood DNA Tubes be shipped?
FAQ ID - 3489
How long can the PAXgene Blood DNA Tube be kept at room temperature? What is room temperature?
FAQ ID - 3492
Which sample types can be processed using the PAXgene Blood miRNA protocols?
FAQ ID - 3478
What is the shelf life of the PAXgene Blood DNA Tube prior to blood collection?
FAQ ID - 3488
How should blood samples drawn into the PAXgene Blood DNA Tubes be stored?
FAQ ID - 3490
Can the miScript II RT Kit be used to quantify piRNAs?
FAQ ID - 3432
Can the miScript Plant RT Kit be used to quantify piRNA expression?

FAQ ID - 3434

Can the miScript Plant RT Kit be used to quantify animal miRNAs?
FAQ ID - 3433
Does the miScript Plant RT Kit contain controls?
FAQ ID - 3435
What is the composition of the 5x miScript Plant RT Buffer?
FAQ ID - 3437
Are any of the miScript II RT Kit components interchangeable with the miScript Plant RT Kit components?
FAQ ID - 3436
What should I use to isolate total RNA from plant tissues and what is the maximum recommended amount of tissue input?
FAQ ID - 3438
Are miScript Primer Assays available for plant mature miRNAs?
FAQ ID - 3439
Are plant miScript Primer Assay designs wet-bench validated?
FAQ ID - 3440
Can you describe the adaptor found in the 10x miScript Plant Adaptor?
FAQ ID - 3442
Are miScript Primer Assay included in miScript miRNA PCR Arrays the same primers as the miScript Primer Assays available in individual tubes?
FAQ ID - 3441

 

What is the amplicon size of PCR products generated with the miScript Plant qPCR System?
FAQ ID - 3443
What is the recommended RNA and RNA input range for the miScript Plant RT Kit?
FAQ ID - 3446
Can cDNA synthesized with the miScript Plant RT Kit be used in real-time PCR with TaqMan® MicroRNA Assays?
FAQ ID - 3445
Can the PAXgene RNA stabilization solution/blood mixture from the PAXgene Blood RNA Tube be disposed of by adding bleach?
FAQ ID - 3469
Can I scale up reagents if I want to use more sample with the MagAttract HMW DNA Kit (cat. no. 67563)?
FAQ ID - 3448
How can I calculate the number of bacterial cells that are present in a sample using the Microbial DNA qPCR Assays?
FAQ ID — 3411
What is the difference between the Microbial DNA qPCR Assays and the QuantiFast Pathogen +IC Kits?
FAQ ID — 3410
What RNA yields are expected with the PAXgene Blood RNA kit?
FAQ ID - 3464
Can the PAXgene 96 Blood RNA Kit be used for diagnostic or prognostic procedures?
FAQ ID - 3471
What are possible reasons for blood draws with lower than expected blood volume?
FAQ ID - 3462
How long can the PAXgene Blood RNA tubes be kept frozen at –20°C or –70°C?
FAQ ID - 3463
Can the PAXgene Blood RNA System be used to isolate DNA?
FAQ ID - 3466
Can the PAXgene Blood RNA System be used to isolate viral RNA?
FAQ ID - 3465
Can the PAXgene Blood RNA System be used with animal blood samples?
FAQ ID - 3461
Can I use DNA isolated from an AllPrep DNA/RNA Kit with qBiomarker Copy Number PCR Arrays and Assays
FAQ ID — 3425
What is the Globally Harmonized System of Classification and Labelling of Chemicals (GHS) and what is the impact for me as a customer?
FAQ ID - 3428
What transfection method should I use with the SureSilencing shRNA Plasmids?
FAQ ID -2901
What are the software requirements to run the QIAlink software package?
FAQ - 3426
What is the data analysis method, and where can I find it for the qBiomarker Somatic Mutatation PCR arrays?
FAQ ID -2915
What is the nature and target of the Internal Control supplied with Certal Residual DNA Detection Kits?
FAQ ID -2974
What is the principle for qBiomarker Somatic Mutation PCR Array data analysis?
FAQ ID -2916
What is the label of the probe that is used in the Internal Control Assay to detect the Internal Control supplied with Certal Residual DNA Detection Kits?
FAQ ID -2976
Were all assays on the qBiomarker Somatic Mutation PCR Arrays bench-validated?
FAQ ID -2912
What are the advantages of RT-PCR based somatic mutation PCR arrays and assays compared to other platforms?
FAQ ID -2921
What are the control features on the qBiomarker Somatic Mutation PCR Arrays?
FAQ ID -2914
What happens if the starting volume is too low when using QIAsymphony mericon Bacteria Kit?
FAQ ID -2962
What happens if the starting volume is too high when using QIAsymphony mericon Bacteria Kit? Can spillage occur when the sample is initially transferred?
FAQ ID -2963
What effects can be predicted with poor sample quality? How robust is the platform with questionable sample quality (such as FFPE samples)?
FAQ ID -2918
What elution formats can be used for the QIAsymphony PAXgene Blood RNA protocol?
FAQ ID -2986
What elution formats can be used for the QIAsymphony DSP Virus/Pathogen protocols?
FAQ ID -2937
When should I choose EasyXpress Disulfide Insect Kits?
FAQ ID -2971
Which elution volumes can be used for the QIAsymphony DSP Virus/Pathogen protocols?
FAQ ID -2938
What sample volumes can be used with the tissue HC and LC protocols on the QIAsymphony SP?
FAQ ID -2992
What types of templates can be used for expression of antibody fragments?
FAQ ID -2964
Why do I have to use an ACS (Assay Control Set)?
FAQ ID -2933
Which kit size should be used for each QIAsymphony DSP Virus/Pathogen protocol?
FAQ ID -2930
Will mitochondrial DNA also be isolated using the DNA tissue protocols and QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP?
FAQ ID -2991
Which sample types can be processed using the QIAsymphony PAXgene Blood RNA Protocol?
FAQ ID -2981
What pathways and diseases are currently covered for the qBiomarker Somatic Mutation PCR Arrays?
FAQ ID -2922
Why does the template for EasyXpress Disulfide Insect Kits need to have a signal sequence (such as the mellitin signal sequence as provided by the EasyXpress Linear Template Fab Kit primers)?
FAQ ID -2967
What sample input formats can be used for the QIAsymphony DSP Virus/Pathogen protocols?
FAQ ID -2936
What is the starting volume for the QIAsymphony mericon Bacteria Kit?
FAQ ID -2956
What is the Limit of Detection (LOD) for an ELISArray Kit?
FAQ ID -2907
What is the sensitivity of the assays contained on the qBiomarker Somatic Mutation PCR Arrays?
FAQ ID -2910
What is the linear range of the Multi-Analyte ELISArray Kits?
FAQ ID -2909
What are the expected DNA yields from tissue processed on the QIAsymphony SP?
FAQ ID -2990
What is the maximum number of GOIs that I can detect with the Type-it CNV Probe Master Mix in the same qPCR reaction?
FAQ ID -2998
What are the recommended lysis times for tissue samples on the QIAsymphony SP?
FAQ ID -2993
What are the technological principles behind somatic mutation PCR arrays and assays?
FAQ ID -2924
What PCR machine do I need to use to run such a qBiomarker Somatic Mutation PCR Arrays?
FAQ ID -2917
What method(s) do you recommend as an alternative way to validate the mutations identified on the somatic mutation PCR arrays?
FAQ ID -2920
What sample types can I test on the qBiomarker Somatic Mutation PCR Arrays?
FAQ ID -2919
What sample volumes can be processed using the QIAsymphony DSP Virus/Pathogen protocols?
FAQ ID -2929
What sample types should be processed with the low content (LC) and high content (HC) protocols for DNA extraction on the QIAsymphony SP?
FAQ ID -2989
What samples can the QIAsymphony mericon Bacteria Kit be used for?
FAQ ID -2958
What sample types can be processed using the QIAsymphony DSP Virus/Pathogen protocols?
FAQ ID -2928
Do you have stability data for RNA isolated with the QIAsymphony PAXgene Blood RNA Kit?
FAQ ID -2983
What sample types can be processed using the tissue protocols and the QIAsymphony DSP DNA Mini Kit?
FAQ ID -2925
Do you need to pre-treat food samples before DNA isolation using the QIAsymphony mericon Bacteria Kit on the QIAsymphony SP?
FAQ ID -2957
Do the samples require pretreatment before loading onto the instrument with the QIAsymphony PAXgene Blood RNA Protocol?
FAQ ID -2982
How do you decide on the qBiomarker Somatic Mutation PCR Arrays to include on the pathway arrays and disease arrays?
FAQ ID -2913
Do the SureSilencing shRNA Plasmids contain inducible promoters?
FAQ ID -2904
How many freeze-thaw cycles can the Internal Control supplied with Certal Residual DNA Detection Kits tolerate?
FAQ ID -2975
How many mutations can I profile per sample on the qBiomarker Somatic Mutation PCR Array?
FAQ ID -2911
How can I estimate the size of my protein based on the length of the respective cDNA?
FAQ ID -2952
How do I use the qBiomarker Somatic Mutation PCR Arrays?
FAQ ID -2923
Can I use the Type-it CNV kits and Reference Assays for CNV studis in mice?
FAQ ID -2997
Can the Certal CHO Positive Control DNA be used to create a qPCR standard for relative quantification?
FAQ ID -2979
Can I use lysates or tissue homogenates with the ELISArray kits?
FAQ ID -2905
Can the EasyXpress Linear Template Fab Kit be used to express entire IgGs?
FAQ ID -2966
Can I use TaqMan® Copy Number Assays from LIFE Technologies in combination with the Type-it CNV Probe kit?
FAQ ID -2999
Can I use the SureSilencing shRNA Plasmids with primary cells or macrophages or for injection into live animals?
FAQ ID -2902
Do I have to use an internal control with each QIAsymphony DSP Virus/Pathogen Kit protocol run?
FAQ ID -2932
Do samples require pretreatment before they are loaded onto the QIAsymphony instrument?
FAQ ID -2931
Can the QIAsymphony DSP Virus/Pathogen system be used for diagnostic or prognostic procedures?
FAQ ID -2939
Do the ELISArray Kits work on species other than human, mouse or rat?
FAQ ID -2906
Does the QIAsymphony mericon Bacteria Kit contain an extraction control?
FAQ ID -2961
Is it possible to simultaneously isolate DNA and RNA from Saliva Stabilized in RNAprotect® Saliva Reagent?
FAQ ID -2996
Is it possible to use templates with signal sequences with EasyXpress Disulfide E. coli Kits?
FAQ ID -2969
Is food DNA co-purified using the QIAsymphony mericon Bacteria Kit?
FAQ ID -2959
Is it possible to introduce an affinity tag using the EasyXpress Linear Template Fab Kit?
FAQ ID -2972
Is it possible to isolate DNA from Saliva Stabilized in RNAprotect® Saliva Reagent?
FAQ ID -2995
On what cyclers have Certal Residual DNA Detection Kits been validated?
FAQ ID -2978
On which cyclers can Certal Residual Detection Kits be used?
FAQ ID -2973
Should I use the SureSilencing shRNA Plasmids with the GFP, neomycin, or hygromycin, or puromycin resistance marker?
FAQ ID -2900
Is it possible to use the native signal sequence of antibodies with EasyXpress Disulfide Insect Kits?
FAQ ID -2968
Is RNA co-purified using the QIAsymphony mericon Bacteria Kit?
FAQ ID -2960
How much DNA does a bacterial cell contain?
FAQ ID -2947
How many samples can be processed with the QIAsymphony DSP Virus/Pathogen Kits?
FAQ ID -2935
How many reactions can be performed with one QIAsymphony mericon Bacteria Kit?
FAQ ID -2955
How should the samples be stored before purification of RNA with the QIAsymphony PAXgene Blood RNA Protocol?
FAQ ID -2984
How should the samples be stored before purification with the QIAsymphony DSP Virus/Pathogen Kits?
FAQ ID -2934
If a cycler from Applied Biosystems is used in combination with the Certal Residual DNA Detection Kit, should dye calibration for MAX be performed?
FAQ ID -2977
How much sample is required for each well for an ELISArray Kit?
FAQ ID -2908
How much starting material can be used with the QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP?
FAQ ID -2927
3198 - What is the optimal DNA concentration for the Investigator DIPlex Kit?
3194 - What DNA fragment sizes are required for the Investigator DIPlex kit?
3195 - Where can I find the Population Data for the Investigator DIPlex kit?
3196 - What is the DIPsorter Freeware?
3188 - How many His residues are required for successful purification on Ni-NTA resins?
3190 - What is the smallest elution volume for RNeasy Mini Spin column?
Why does the QIAcube sometimes fail to pick up the tips or pick up wrong tips?
FAQ ID -3131
The kit says I can use up to 30mg of tissue. When I used 15mg I got a higher yield than when I used 30mg. Why?
FAQ ID -3117
I want to purify RNA from heart tissue. What is the best kit to use?
FAQ ID -3116
Why does the upper aqueous phase look pinkish when purifying RNA from fatty tissue?
FAQ ID -3118
Is it possible to partially use RNeasy 96 plates and use the plate multiple times?
FAQ ID -3124
Can I use the QIAGEN deparaffinization solution with the IDH 1/2 RGQ PCR kit?
FAQ ID -3100
Can I use stained slide materials with IDH 1/2 RGQ PCR kit?
FAQ ID -3101
What is the standard format for Flexiplate siRNAs delivery?
FAQ ID -3104
How many transfections can be performed with Flexiplate siRNAs?
FAQ ID -3105
Where to look for ordering Custom siRNAs online?
FAQ ID -3106
What is the difference between HiPerFect and HiPerFect HTS?
FAQ ID -3108
What are the miScript Controls that can be used with miScript HiSpec Buffer or miScript HiFlex Buffer?
FAQ ID -3111
What is the amplicon size of the PCR product when using the miScript primer assay?
FAQ ID -3156
What is the difference between EpiTect Bisulfite Kit and EpiTect Plus Bisulfite Kit?
FAQ ID -3167
What are the stopping points in the QIAamp protocol?
FAQ ID -3157
What are the benefits of PyroMark Q24 Advanced versus PyroMark Q24?
FAQ ID -3158
Do you have data on the DNA yield at different incubation time for the Repli-G Kits?
FAQ ID -3113
Is it possible to upgrade existing PyroMark Q24 instruments to PyroMark Q24 Advanced or do I have to purchase a new instrument?
FAQ ID -3162
Is it possible to run therascreen mutation or therascreen Pyro kits on PyroMark Q24 Advanced system?
FAQ ID -3161
Which operating system is compatible with PyroMark Q24 Advanced application software?
FAQ ID -3164
What is the adapted annealing protocol for PyroMark Q24 Advanced procedure?
FAQ ID -3166
What is the miRNA yield from the miRNeasy Serum/Plasma Kit?
FAQ ID -3171
What is the RNA size distribution recovered from the miRNeasy Serum/Plasma Kit?
FAQ ID -3172
What could be a reason for higher viscosity of PCR reaction with STR Plus chemistry?
FAQ ID -3176
Does QIAGEN provide template files for GeneMapper® 5 software?
FAQ ID -3177
Can Rotor-Gene Reports be modified?
FAQ ID -3184
What is the best way to ensure the Rotor-Disc is properly sealed when using the Heat Sealer?
FAQ ID -3179
What to do if there is a loss of communication for the Rotor-Gene Q?
FAQ ID -3180
Aside from the OTV test, what other maintenance should be done for the Rotor-Gene Q?
FAQ ID -3181
Are the miRNA Target Protector & miRNA mimic bench tested? Any guarantee?
FAQ ID - 3186
3187 - Why does the Ni-NTA resin turn reddish-brown after mixing with the lysates?
3191 - Is the Allprep DNA Mini Spin Column the same as the gDNA eliminator column? Can I interchange these columns?
3199 - Which species can be used with the GeneRead rRNA depletion kit?
3192 - Is QIAGEN Protease compatible with Buffer ATL?
What is the bacterial ribosomal RNA recommended to use in the miRNeasy Serum/Plasma Protocol?
FAQ ID -3173
3193 - Where can I get an RNase-free syringe that is recommended for reconstituting QIAGEN's RNase-free DNase (cat. no. 79254)?
Would co-transfection with the plasmid be expected to increase/decrease the transfection efficiency significantly?
FAQ ID -3136
How can I know whether there is any genomic DNA present in my purified plasmid?
FAQ ID -3134
How do I know whether my miRNA has been successfully purified and how much is expected?
FAQ ID -3135
Is it possible to use RNAprotect Cell Reagent to stabilize buffy coat?
FAQ ID -3139
Has QIAGEN ever tested Proteinase K treatment for a time period longer than overnight?
Is RNAprotect also suitable for the stabilization of miRNA?
FAQ ID -3143
Does RNAprotect affect morphology of tissues?
FAQ ID -3142
Are miScript primer assays bench-validated?
FAQ ID -3145
What is the maximum cDNA length that can be synthesized by QuantiTect Reverse Transcription Kit?
FAQ ID -3146
Why does the DNeasy mericon Food extraction kit use a QIAquick column and not DNeasy column?
FAQ ID -3148
What are the minimal and maximum fragment sizes for the QIAquick column in the mericon DNeasy kit?
FAQ ID -3147
Does RNase-free DNase I affect the isolation of dsRNA?
FAQ ID -3153
Can single-stranded DNA be removed by gDNA Eliminator columns?
FAQ ID -3152
How is total RNA purified with RNeasy kits free of genomic DNA contamination?
FAQ ID -3154
Is it possible to quantify protein content in RLT lysates?
FAQ ID -3155
What primary cell lines have been tested with HiPerFect Transfection Reagent?
FAQ ID -3137
Is it possible to use the protein resuspended in ALO buffer for downstream BCA assay and Bradford assay?
FAQ ID -3138
What are the miScript Controls that can be used with miScript HiFlex Buffer ONLY?
FAQ ID -3112
How long can I store an RLT lysate?
FAQ ID -3115
Do you have a protocol for the isolation of genomic DNA from yeast/fungi directly from Blood?
FAQ ID -3150
Is there cross talk between channels in the Rotor-Gene Q?
FAQ ID -3185
3197 -Where can I find the Population Data for the Investigator HDplex kit?
Can QIAzol aqueous phase be used with the gDNA eliminator columns in RNeasy Plus mini kit?
FAQ ID -3151
What is in the HiSpec Buffer that selectively synthesizes cDNA from mature miRNAs ONLY?
FAQ ID -3168
What is the optimal DNA concentration for the Investigator ESSplex Plus Kit?
FAQ ID -2646
Why do I see low, poor, or sub-standard amplification efficiency in my qRT-PCR assay?
FAQ ID -2695
What is the difference between adding the Internal Control (IC) template used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit to the amplification reaction versus adding the IC template at the extraction step?
FAQ ID -2600
Is the QuantiFast SYBR Green PCR Kit compatible with the ViiA7 cycler from Applied Biosystems?
FAQ ID -2652
What are the most reliable methods for preparing high-quality RNA from cell or tissue samples, for use in gene expression analysis experiments?
FAQ ID -2657
What should the cycler set-up be for a duplex reaction with the pathogen assay (FAM) and the Internal Control assay (MAX) on ABI instruments, when using the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2610
What should the cycler set-up be for a duplex reaction with the pathogen assay (FAM) and the Internal Control assay (MAX) on Mx instruments from Stratagene (Agilent), when using the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2609
What should I do if I suspect that my RNA preparation contains RNase contamination?
FAQ ID -2661
What real-time cycler should I use for my qPCR experiments?
FAQ ID -2670
Which DNA/RNA extraction kits were tested in combination with the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2606
What should the Rotor-Gene Q cycler settings be for a duplex reaction with the pathogen assay (FAM) and the Internal Control assay (MAX), when using the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2608
What testing should be performed to assess the quality of an RNA sample?
FAQ ID -2660
For the QuantiFast Pathogen RT-PCR +IC kit, does the Internal Control also control for the reverse transcription reaction?
FAQ ID -2601
Do we have data on long term stability and storage of DNA purified on BioRobot M48?
FAQ ID -2611
Following thermal cycling, reaction volume has evaporated from some of the wells of my qPCR assay plate. What should I do?
FAQ ID -2683
How can I predict the percent qPCR signal due to contaminating DNA, for a given qPCR assay, and its matching NRT control?
FAQ ID -2688
How can I ensure that reaction volume is not lost due to evaporation during thermal cycling?
FAQ ID -2679
How can I determine whether amplification occurs from mRNA-derived cDNA or from genomic DNA contamination?
FAQ ID -2687
Can the Internal Control DNA or RNA be added directly to the sample?
FAQ ID -2602
Do the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit contain ROX in the master mix?
FAQ ID -2605
Are primers available that only detect mitochondrial DNA encoded genes and not nuclear genomic DNA encoded genes?
FAQ ID -2680
Do I need to run a standard curve before the actual PCR array experiment?
FAQ ID -2664
Does the amount of Internal Control to be spiked into the lysis buffer or lysate depend on the elution volume only?
FAQ ID -2604
May I try the data analysis tool without using your PCR array kit?
FAQ ID -2698
Is the QuantiFast Multiplex PCR +R Kit compatible with the ViiA7 cycler from Applied Biosystems?
FAQ ID -2653
Is the QuantiFast Probe PCR +ROX Vial Kit compatible with the ViiA7 cycler from Applied Biosystems?
FAQ ID -2651
Is it good to pool multiple RNA replicates to detect expression changes that are consistently reproducible?
FAQ ID -2663
How important is the RNA purification process, for obtaining reliable qRT-PCR results?
FAQ ID -2655
Is a special processing protocol needed?
FAQ ID -2523
Is it possible to archive PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue blocks?
FAQ ID -2524
The disruption of my sample worked well with the TissueLyser LT, but I am not satisfied with the RNA yield. What do you suggest?
FAQ ID -2555
What are the dimensions of the TissueLyser LT?
FAQ ID -2545
What is the average RNA yield from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -2534
On which cyclers can the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit be used?
FAQ ID -2596
What are the storage conditions for the mericon Assay kits?
FAQ ID -2540
What is in the elution buffer in the PAXgene Bone Marrow RNA kit?
FAQ ID -2511
Is it possible to microdissect PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissues?
FAQ ID -2531
Is the performance of the HiPerFect HTS Reagent affected if it has accidentally been stored at°C?
-20
Is it possible to embed PAXgene Tissue fixed and stabilized samples in Optimal Cutting Temperature (OCT) medium for freezing?
FAQ ID -2525
Is the morphology after H&E staining comparable to formalin-fixed samples?
FAQ ID -2526
What RNA yields are expected with the PAXgene Bone Marrow RNA System?
FAQ ID -2506
What is the maximum tissue size that can be fixed in a PAXgene Tissue Container?
FAQ ID -2518
What is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?
FAQ ID -2538
What is the expected amplicon size when using the mericon Assay kits?
FAQ ID -2544
What is the typical amount of tissue used for disruption with the TissueLyser LT?
FAQ ID -2552
What is the longest period you recommend to leave out the dilute HiPerFect HTS at RT before use?
FAQ ID -2560
What kind of sample tubes are recommended to use with the TissueLyser LT?
FAQ ID -2553
What is the purity of nucleic acids extracted with the PAXgene Tissue kits?
FAQ ID -2533
Can the PAXgene Bone Marrow RNA System be used to isolate genomic DNA or viral RNA and DNA?
FAQ ID -2507
Can the PAXgene Bone Marrow RNA System be used with animal bone marrow or with blood samples?
FAQ ID -2501
Can the PAXgene RNA stabilization solution/bone marrow mixture from the PAXgene Bone Marrow RNA Tube be disposed of by adding bleach?
FAQ ID -2515
Can sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for other histochemical staining techniques, such as PAS?
FAQ ID -2530
Can the PAXgene Bone Marrow RNA System be used for other human body fluids than bone marrow samples or cultured cells of human origin?
FAQ ID -2502
Can the sample tubes and the TissueLyser LT Adapter be cooled with liquid nitrogen?
FAQ ID -2549
Can the TissueLyser LT Adapter be removed for cleaning?
FAQ ID -2546
Can the samples on the TissueLyser LT be protected from degradation of nucleic acids or proteins?
FAQ ID -2548
When using the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit, what should the fluorescent label of the probe be for the customer-defined assay to detect the pathogen?
FAQ ID -2594
When using HiPerFect HTS Reagent is it necessary to optimize amount of siRNA and transfection reagent?
FAQ ID -2556
What sample numbers can be processed on the TissueLyser LT?
FAQ ID -2547
What size bead should I use for tougher samples with the TissueLyser LT?
FAQ ID -2554
When performing the Internal Control assay for the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit, what channel or filter can be used to detect MAX?
FAQ ID -2597
Why is the fluorescence signal of the Internal Control on my Rotor-Gene Q very low?
FAQ ID -2574
Can specific types of white blood cells be enriched from a PAXgene Bone Marrow RNA Tube prior to the RNA isolation?
FAQ ID -2508
Can bone marrow aliquots other than 2 mL be pipetted into the PAXgene Bone Marrow RNA Tube?
FAQ ID -2503
Are the Internal Controls used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit homologous to a known target?
FAQ ID -2598
Can I use water to elute the RNA in the PAXgene Bone Marrow RNA Kit?
FAQ ID -2512
Can PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for in situ hybridization?
FAQ ID -2529
Can bone marrow from leukemia patients with elevated numbers of white blood cells be processed with the PAXgene Bone Marrow RNA System?
FAQ ID -2510
Can HiPerFect HTS be used for miRNA?
FAQ ID -2561
Can HiPerFect HTS Reagent be used for transfecting shRNAs?
FAQ ID -2559
How long does the sample disruption with the TissueLyser LT take?
FAQ ID -2550
How many times can the Internal Controls used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit be freeze-thawed?
FAQ ID -2599
How long can I store the dilutions of the Control DNA Z1 standards at 2°C?
-8
How long can primer and probes from the mericon Assay kits be kept at°C once reconstituted?
-20
How should the PAXgene Bone Marrow RNA Tubes be frozen after collection and for how long?
FAQ ID -2505
I routinely heat an aliquot of the RNA eluate prior to my RT reaction. Do I still need to heat the eluate after the PAXgene Bone Marrow RNA preparation?
FAQ ID -2513
How much time may the aspirated bone marrow sample be allowed to incubate before pipetting it into the PAXgene Bone Marrow RNA Tube?
FAQ ID -2504
Do you have FACS or Fluoro-Microscopy data using HiPerFect HTS Reagent for delivery of siRNA?
FAQ ID -2557
How is stability and performance of these mericon Assay kits guaranteed?
FAQ ID -2542
How are the supernatants resulting from the first two spin steps of the PAXgene Bone Marrow RNA kit purification procedure disposed?
FAQ ID -2516
What are typical yields of bisulfite converted DNA when using the EpiTect Plus Bisulfite Kits?
FAQ ID -2408
What batch sizes can be selected with the PAXgene Blood RNA MDx Kit?
FAQ ID -2491
What are the labels of the probe which is used in the Internal Control Assay for detection of the IC?
FAQ ID -2449
What is the detection limit for the QuantiFast Pathogen + IC kits?
FAQ ID -2453
In which solution can I keep my protein for the NexTal Cubic Phase kits?
FAQ ID -2416
What is the difference between QuantiTect Virus Kit and the new QuantiFast Pathogen +IC Kits?
FAQ ID -2448
Is there a critical step during upfront sample handling when using the PAXgene Blood RNA MDX kit?
FAQ ID -2493
Which EpiTect Bisulfite Plus kit do you recommend for blood samples, cells, and tissues?
FAQ ID -2411
What is the nature of the Internal Control in the QuantiFast Pathogen + IC kit?
FAQ ID -2450
Where can I find general information about RT-PCR and PCR arrays such as information about sample preparation, reverse transcription, quantitative PCR, real-time PCR, and PCR arrays?
FAQ ID -2446
What is the maximum number of batches for the QIAsymphony PAXgene Blood RNA protocol for a single run?
FAQ ID -2498
Why should I work with diluted or undiluted screens?
FAQ ID -2419
Why should I use the EpiTect Plus FFPE Bisulfite Kit instead of isolating FFPE DNA first and then doing the bisulfite conversion?
FAQ ID -2412
What is the difference between sponge, cubic and lamellar phase of the NeXtal CubicPhase crystallisation?
FAQ ID -2420
Where are the somatic mutations published?
FAQ ID -2429
What should be the concentration of my protein for the Nextal Cubic Phase products?
FAQ ID -2417
What kind of control samples should I use for the particular iPSC-qBiomarker Arrays I have ordered?
FAQ ID -2441
What is the range indicated by 'Std. Error' on 'Results' page?
FAQ ID -2456
Can the PAXgene Blood RNA MDx Kit be used with both the BioRobot Universal System and the BioRobot MDx?
FAQ ID -2489
Can the Internal Controls be ordered separately in the QuantiFast Pathogen + IC kit for use during purification?
FAQ ID -2451
Can the PAXgene Blood RNA MDx Kit be used for diagnostic or prognostic procedures?
FAQ ID -2488
Can the QIAsymphony PAXgene Blood RNA System be used for diagnostic or prognostic procedures?
FAQ ID -2496
Can the PAXgene Blood miRNA System be used for diagnostic or prognostic procedures?
FAQ ID -2494
At what temperature can I perform the NexTal Cubic Phase experiment and incubate the plate?
FAQ ID -2415
How are the different mutations for a single gene normalized on the qBiomarker Somatic Mutation PCR array?
FAQ ID -2428
How can I check DNA sample quality before using the Somatic Mutation PCR Arrays & Assays?
FAQ ID -2430
How much DNA starting sample is needed for analysis with a Somatic Mutations PCR Array? And Assay?
FAQ ID -2431
How do I get started using the NeXtal CubicPhase kits?
FAQ ID -2413
How pure should my protein be with the NexTal CubicPhase kits?
FAQ ID -2414
How many samples can be processed with the QIAsymphony PAXgene Blood RNA Kit?
FAQ ID -2497
How many samples can be processed with the PAXgene Blood RNA MDx Kit?
FAQ ID -2490
In the REST software, what is the range indicated by 'Std. Error' on the 'Results' page?
FAQ ID -2455
How many PAXgene Blood RNA Tubes can be processed per PAXgene 96 Blood RNA Kit well plate?
FAQ ID -2485
If less than 48/96 samples are being processed, is it possible to leave positions empty with the PAXgene Blood RNA MDx Kit?
FAQ ID -2492
How does the DNA Protection work in the EpitTect Bisulfite kits?
FAQ ID -2410
How is the hypothesis test performed in the REST software?
FAQ ID -2459
How do I determine if my gene expression results are statistically significant in the REST software?
FAQ ID -2458
How many different genes and gene mutations are on a Somatic Mutation PCR Array?
FAQ ID -2427
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