常见问题

 

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3329 - When using REPLI-g, if the starting material is a single sorted cell, could mitochondrial DNA (mtDNA) be amplified?
3331 - Should the REPLI-g amplified mtDNA be cleaned up before sequencing?
3332 - Is there a method that can distinguish between non-amplified DNA and REPLI-g amplified DNA?
3333 - When using REPLI-g, what is the purpose of the DTT added to the denaturation buffer in the cells/blood protocol?
What sample types can be tested on the arrays/assays?
FAQ ID — 3399
What are the minimum sample requirements for Microbial DNA qPCR kits?
FAQ ID — 3393
Can I use the Microbial DNA-Free Water and Microbial qPCR Mastermix if they have been opened more than 3 times?
FAQ ID — 3398
3353 - What is the composition of the elution buffer in the Ni-NTA Fast Start Kit?
FAQ ID - 3353
3352 - What are the size ranges of Ni-NTA particles?
FAQ ID - 3352
3354 - What type and amount of resin is packed into the Ni-NTA Spin columns from the Ni-NTA Spin Kit?
FAQ ID - 3354
3341 - How are the EpiTect DNA controls produced and tested?
FAQ - 3341
3351 - What is the upper limit for protein size that can bind to Ni-NTA agarose resin?
FAQ ID - 3351
3344 - What is the difference between the EpiTect Plus DNA Bisulfite kit compared to the classic kit?
FAQ - 3344
3345 - What is the difference between the high and low concentration EpiTect Bisulfite Conversion protocol?
FAQ - 3345
3311 - Can QuickLyse miniprep kits be used with QIAVac 24 Plus?
3313 - What is the endotoxin level for the QIAGEN Plasmid Plus kits?
3312 - For DirectPrep 96 miniprep, is it possible to use a half plate and save the other half for later prep?
3314-What is the concentration of RNase A sold separately?
3316-What is the concentration of the reconstituted QuantiFast Probe Assay?
3325 - What are the catalog numbers for the packing materials for the QIAcube?
3324 - Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube?

 

3335 - Can I use the DNA directly from enzymatic reaction for Epitect Bisulfite treatment with the Epitect Bisulfite kit (cat. no. 59104)?
3336 - Can I use the linearized DNA for Epitect Bisulfite treatment with the Epitect Bisulfite kit?
3339 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) negatively impact subsequent NGS sequencing?
3337 - Which method is used for QIAGEN sequencing service?
3338 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) have 3'-hydroxyl and 5'-phosphate groups?
3343 - Why is it difficult to quantify DNA using UV/Vis spectroscopy and fluorescence methods following EpiTect Bisulfite Conversion? How best do you quantify it?
FAQ - 3343
3342 - How is the unmethylated unconverted EpiTect DNA control to be used?
FAQ - 3342
3315-What is the concentration of the reconstituted QuantiTect Primer Assay (200)?
3322 - What is the concentration of the reconstituted miScript Primer Assay (100) and miScript Precursor Assay (100) tubes?
3317 - Do you have a protocol for isolation of genomic DNA from cell cultures using the QIAamp cador Pathogen Mini Kit?
3318 - What guidelines should I follow in designing primers for use with the EpiTect MSP Kit?
3320 - What is the sensitivity of the Type-it HRM PCR Kit?
3321 - What is the purpose of the C. elegans miR-39 spike-in control in the miRNeasy Serum/Plasma kit?
3318 - What is the preferred amplicon size for performing PCR on DNA amplified with the EpiTect Whole Bisulfitome Kit?
3305 - What is the minimal amount of starting material required for using the SBTexcellerator HLA Kits?
3306 - What is the optimal sample DNA concentration for the Investigator ESSplex SE Plus Kit?
3304 - What is the maximum DNA input volume that can be used in the Investigator ESSplex SE Plus Kit?
3310 - Which settings for the filter set Any5Dye must be adjusted before running the spectral calibration for Investigator ESSplex SE Plus Kit?
3308 - Where can I find the template files for the Investigator ESSplex SE Plus Kit?
3309 - Which filter set should be used with Investigator ESSplex SE Plus Kit?
What additional equipment is needed to purify RNA using the PAXgene 96 Blood RNA Kit?
FAQ ID -499
3300 - Can isopropanol or methanol be used in place of ethanol in RNeasy kits?
3301 - Do you have any information or guidelines regarding the choice of reference genes for real-time PCR?
3302 - How long can I store my purified RNA?
3210 - Can the 4-16K Centrifuge be used with a 208 V outlet?
3211 - What is the maximum speed of the plate rotor 2 x 96 in the 4-16 or 4-16K?
3212 - What is the minimum size of DNA fragment that can be isolated with QIAamp DNA Mini kit?
3299-Can Buffer ER from the Endofree Plasmid kits be used instead of the Buffer ETR in QIAGEN Plasmid Plus kits?
3213 - How much ethanol should be added to Buffer AP3/E (renamed to Buffer AW1) and Buffer AW (renamed to Buffer AW2) in the DNeasy Plant kit?
3202 - Can GeneRead rRNA depletion kit be used to deplete rRNA from miRNA isolated using miRNeasy kit?
3200 - What is the Maximum/Minimum amount of RNA that can be used with GeneRead rRNA depletion kit?
3201 - What quality of RNA can be used with the GeneRead rRNA depletion kit?
3204 - How strict is the purification cut-off with GeneRead Size Selection kit?
3203 - Is the RNeasy MinElute cleanup kit required for the GeneRead rRNA depletion kit?
3205 - Can GeneRead Library preparation kits be used on species other than human mouse and rat, for example, plant species and microbial species?
3206 - Can our GeneRead Library preparation kits be used with the SoLiD Platform?
3248 - What is the difference between sponge, cubic and lamellar phase?
3254 - When using the Nextal Cubic Phase products how do I know in which phase the sample is? What will I see?
3255 - When using the NexTal Cubic Phase products, should I work with diluted or undiluted screens?
3227 - In which solution can I keep my protein with the NexTal Cubic Phase kits?
3225 - How pure should my protein be for the NexTal CubicPhase kits?
3217 - How long can I store my purified RNA?
3292-How do I start using the NeXtal CubicPhase kits?
3295-What is the difference between SBTexcellerator HLA Core, Extended and Full Kits?
3240 - What are the main differences between the qBiomarker PCR Arrays and the RT2 Profiler PCR Arrays?
3237 - I would like to order the QuantiFast Probe Assays without master mix. Is this possible?
3233 - Is the yield and quality of RNA and DNA purified with the AllPrep DNA/RNA FFPE Kit comparable to yield and quality of nucleic acids purified separately with other methods.
3265 - Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)?
3298-Will the sequence of the QuantiFast Probe Assays be provided?
3262 - Which thermal cyclers are validated for use with the Investigator Nonaplex ESS Kit?
3263 - Why are samples centrifuged at 4°C after the addition of chloroform when using the RNeasy Plus Universal Mini Kit?
3241 - What are the recommended storage conditions of the QuantiFast Probe RT-PCR Plus Kit components?
3264 - Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA?
3238 - What are important points regarding the genomic DNA wipeout buffer in the QuantiFast Probe RT-PCR Plus Kit?
3245 - What is the concentration of the primers and the probe in a reaction using the QuantiFast Probe Assays?
3239 - What are the differences between the existing QuantiFast Probe RT-PCR Kit and the new Plus Kit?
3247 - What is the correct matrix to use with the Investigator Nonaplex ESS Kit?
3243 - What does the Investigator Nonaplex ESS Kit contain?
3244 -What is the average length of a QuantiFast Probe Assay?
3231 - Is there a size limit to the protein I can crystallize with the NexTal Cubic Phase products?
3228 - Is it also possible to isolate miRNA with the AllPrep DNA/RNA FFPE Kit?
3232 - Is the RNeasy Plus Universal Mini Kit suited for adipose, brain, and other fatty tissues?
3229 - Is it necessary to perform calibration steps for the use of the MAX dye in duplex RT-PCR experiments on different cyclers?
3236 - I use cultured cells as starting material. Can I also use the RNeasy Plus Universal Kit?
Is there also a miRNeasy Plus Universal Kit available?
3261 - Which purification kits are recommended for the therascreen Pyro kits?
3258 - Which filter set and settings should be used with the Investigator Nonaplex ESS Kit?
3260 - Which purification kits are recommended for the Pyro kits?
3259 - Which probe labels are available for the QuantiFast Probe Assays?
3296-Why does the A peak reduction factor have to de set to 0.86 for the codon 61 assay in the therascreen NRAS Pyro Kit?
What plug-ins are available for the therascreen Pyro kits?
FAQ ID - 3251
3249 - What is the optimal DNA volume and concentration for the Investigator Nonaplex ESS Kit?
What plug-ins are available for the Pyrosequencing kits?
FAQ ID - 3250
3252 - What should be the concentration of my protein with the NexTal Cubic Phase products?
3256 - Which crystallization solutions are compatible with lipidic cubic phase crystallization?
3253 - What species are QuantiTect Probe Assays offered for?
3257 - Which deparaffinization method can I use with the AllPrep DNA/RNA FFPE Kit?
Can the insertions in Exon 20 be detected with the therascreen EGFR Pyro Kit?
Can the insertions in Exon 20 be detected with the EGFR Pyro Kit?
FAQ ID - 3279
3277 - Can I use the RNeasy Plus Universal Mini Kit for all types of tissue?
Can QuantiFast Probe Assays be ordered offline via e-mail or fax?
FAQ ID - 3278
3283-Do you have any information or guidelines regarding the choice of reference genes for real-time PCR?
3285-How are the genes in each iPSC qBiomarker PCR Array selected?
3284-For which applications can the RNA isolated with the RNeasy Plus Universal Kit also be used?
Can the QuantiFast Probe Assay detect short fragmented RNA as well as longer fragments of RNA?
Do the RNeasy Plus Universal Mini/Midi kits contain both wash buffers, RW1 and RWT?
FAQ ID - 3282
3270 - Are the primer sets different for the Investigator Nonaplex ESS Kit and ESSplex SE where the loci are the same?
Are the QuantiFast Probe Assays hydrolysis or FRET probes?
FAQ ID - 3271
Are panels and bin sets available for use with GeneMapper ID and GeneMapper ID-X?
FAQ ID - 3267
3275 - Can I use more than 10ng DNA for the PCR reaction for my therascreen Pyro Kits?
Can I use more than 10ng DNA for the PCR reaction for the Pyro kits?
FAQ ID - 3276
3274 - Can I do singleplex PCR with the QuantiFast Probe Assays?
3273 - At what temperature can I perform the experiment with the NexTal Cubic Phase kits?
3272 - Are the QuantiFast Probe Assays wet-lab validated?
3222 - How much DNA/RNA can be isolated with the AllPrep DNA/RNA FFPE Kit?
3218 - How many fragments are contained within the size standard BTO in the Investigator Nonaplex ESS Kit?
3219 - How many freeze-thaw cycles can the master mix contained in the QuantiFast Probe RT-PCR Plus Kit tolerate?
3220 - How many PCR cycles should I use for the Investigator Nonaplex ESS Kit?
3226 - How pure should the chloroform used with the RNeasy Plus Universal Mini Kit be?
3223 - How much sample material can I use for DNA/RNA purification with the AllPrep DNA/RNA FFPE Kit?
3224 - How much time can be saved when using the RNeasy Mini QIAcube Kit?
3289-How does the QuantiFast Probe RT-PCR Plus Kit eliminate genomic DNA contamination?
3290-How do I analyze the data for the particular iPSC qBiomarker Arrays I have ordered?
3288-How does the gDNA eliminator solution of the RNeasy Plus Universal Kit work?
3294-How long can I store my purified DNA/RNA isolated with the AllPrep DNA/RNA FFPE kit?
3286-How can I adjust the ROX concentration in the QuantiFast Probe RT-PCR Plus master mix?
3291-How do I harvest the crystals from meso phase? Are special tools needed with the NexTal Cubic Phase products?
3246 - What is the correct matrix to use with Investigator ESSplex SE Plus Kit?
3297-Why does the PCR for HLA-DRB4 result in 2 amplicons of different size?
3266 - Why are two different exon 2 forward sequencing primers used for the SBTexcellerator HLA-DRB1 Kit?
How can I find out if my gene of interest is expressed in a specific tissue type or cell line?
FAQ ID - 3214
3215 - What is the starting material for SBTexcellerator HLA Kits?
Can I use genomic DNA from fixed samples with qBiomarker Copy Number PCR Arrays?
FAQ ID — 3416
Can I use amplified genomic DNA with qBiomarker Copy Number PCR Arrays?
FAQ ID — 3415
Do the samples require pretreatment before starting the manual protocol or before loading onto the QIAcube instrument?
FAQ ID - 3479
What are the expected yields of RNA from PAXgene Blood RNA Tubes isolated with the PAXgene Blood miRNA kit?
FAQ ID - 3481
Do you have stability data for RNA isolated with PAXgene miRNA Kit?
FAQ ID - 3480
Can the PAXgene Blood DNA Tubes be stored at 4°C?
FAQ ID - 3493
How should I thaw frozen PAXgene Blood DNA Tubes?
FAQ ID - 3495
Can the PAXgene Blood DNA Tube be frozen? If yes, at what temperatures and for how long?
FAQ ID - 3491
Can mitochondrial DNA be isolated with the PAXgene Blood DNA System?
FAQ ID - 3498
3497 - What is the expected purity and size of DNA extracted using the PAXgene Blood DNA kit?
FAQ ID - 3497
Does the DNA extracted using the PAXgene Blood DNA System contain RNA?
FAQ ID - 3499
Can I multiplex the Microbial DNA qPCR Assays?
FAQ ID — 3408
Can I measure virulence factor gene expression?
FAQ ID — 3405
What sample types can I test on qBiomarker Copy Number PCR Arrays?
FAQ ID — 3423
What is the sensitivity for the Microbial DNA qPCR kits?
FAQ ID — 3409
Which probe labels are available for the Microbial DNA qPCR Assays?
FAQ ID — 3407
Are the Microbial DNA qPCR Assays wet-lab verified?
FAQ ID — 3406
Can I measure antibiotic resistance gene expression?
FAQ ID — 3404
What sequences are used to design the Microbial DNA qPCR Assays?
FAQ ID — 3403
Are the assays species-specific?
FAQ ID — 3400
Why is the copy number call lower than expected?
FAQ ID — 3413
Will qBiomarker Copy Number Assays work with heterogeneous samples like mixtures of tumor and normal tissue?
FAQ ID — 3419
Why do qBiomarker Copy Number PCR Arrays have assays in quadruplicate?
FAQ ID — 3414
How should I analyze the data generated from a qBiomarker Copy Number PCR Array experiment?
FAQ ID — 3421
What could have gone wrong if the CT values are unusually high for all wells in a sample?
FAQ ID — 3412
What qPCR mastermix should I use with the qBiomarker Copy Number PCR Arrays and Assays
FAQ ID — 3422
How much DNA should I use on a qBiomarker Copy Number PCR Array?
FAQ ID — 3417
How much DNA should I use in a qBiomarker Copy Number PCR Assay?
FAQ ID — 3418
How do you choose which assays to include on the arrays?
FAQ ID — 3420
What testing should be performed in order to assess the quality of a DNA sample?
FAQ ID — 3424
How many samples can be processed with the PAXgene Blood miRNA Kit?
FAQ ID - 3477
Why have the hazard symbols accompanying products changed?
FAQ ID - 3430
Why is a blood collection set required to be used with the PAXgene Blood RNA tube?
FAQ ID - 3459
As part of my RT procedure I routinely heat an aliquot of the eluate prior to the RT reaction. Do I still need to heat the whole eluate after the RNA preparation?
FAQ ID - 3468
Is there any chance of the PAXgene Blood RNA tube reagent going back into the patient's arm?
FAQ ID - 3460
I need to buy a new computer for my PyroMark Q96. Can I reinstall the PyroMark Q96 software on my new computer or do I need to purchase a new license?
FAQ ID - 3472
Can I reinstall the PyroMark Q24 software on my new computer or following an operating system upgrade, or do I need to purchase a new license?

FAQ ID - 3473

Can the PAXgene Blood DNA Tubes be stored at higher temperatures?
FAQ ID - 3494
What are the expected yields of RNA from PAXgene Blood RNA Tubes using the QIAsymphony PAXgene Blood RNA Kit?
FAQ ID - 3475
What additional equipment is needed to purify RNA using the PAXgene 96 Blood RNA Kit?
FAQ ID - 3474
Is it possible to isolate genomic DNA or viral nucleic acids with the QIAsymphony PAXgene Blood RNA protocol?
FAQ ID - 3476
2. Are there any special considerations for the collection of blood using PAXgene Blood DNA Tubes CE (e.g., use of a blood collection set or discard tube, positioning of the tube, issues with short draws, etc.)?
FAQ ID - 3485
Are the PAXgene Blood DNA Tubes sterile?
FAQ ID - 3487
What is the additive in PAXgene Blood DNA Tubes
FAQ ID - 3486
What is the maximum number of samples for the QIAcube variant of the PAXgene Blood miRNA protocol for a single run?
FAQ ID - 3482
Do I have to use a blood collection set with the PAXgene Blood DNA Tube?
FAQ ID - 3484
Is it possible to isolate genomic DNA or viral nucleic acids with PAXgene Blood miRNA protocol?
FAQ ID - 3483
How should blood samples drawn into PAXgene Blood DNA Tubes be shipped?
FAQ ID - 3489
How long can the PAXgene Blood DNA Tube be kept at room temperature? What is room temperature?
FAQ ID - 3492
Which sample types can be processed using the PAXgene Blood miRNA protocols?
FAQ ID - 3478
What is the shelf life of the PAXgene Blood DNA Tube prior to blood collection?
FAQ ID - 3488
How should blood samples drawn into the PAXgene Blood DNA Tubes be stored?
FAQ ID - 3490
Can the miScript II RT Kit be used to quantify piRNAs?
FAQ ID - 3432
Can the miScript Plant RT Kit be used to quantify piRNA expression?

FAQ ID - 3434

Can the miScript Plant RT Kit be used to quantify animal miRNAs?
FAQ ID - 3433
Does the miScript Plant RT Kit contain controls?
FAQ ID - 3435
What is the composition of the 5x miScript Plant RT Buffer?
FAQ ID - 3437
Are any of the miScript II RT Kit components interchangeable with the miScript Plant RT Kit components?
FAQ ID - 3436
What should I use to isolate total RNA from plant tissues and what is the maximum recommended amount of tissue input?
FAQ ID - 3438
Are miScript Primer Assays available for plant mature miRNAs?
FAQ ID - 3439
Are plant miScript Primer Assay designs wet-bench validated?
FAQ ID - 3440
Can you describe the adaptor found in the 10x miScript Plant Adaptor?
FAQ ID - 3442
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