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Can the QIAamp MinElute Virus Kits be used to extract viral nucleic acid from cell culture supernatant?
FAQ ID -472
Can RNAi experiments be performed in Drosophila?
FAQ ID -436
How is the RNeasy Lipid Tissue Mini Kit different from the RNeasy Mini Kit?
FAQ ID -468
Has RNAi been successful using siRNA in Zebrafish and Xenopus?
FAQ ID -400
Are Northern Blots sensitive enough to detect siRNA-induced gene silencing?
FAQ ID -403
Can I use the RNeasy MinElute Cleanup Kit to clean up my in vitro transcription reaction?
FAQ ID -429
What is the composition of buffer OEB?
FAQ ID -420
Why is the pQE DNA provided in QIAexpress Kits blue in color?
FAQ ID -487
What is the minimum elution volume when using the RNeasy Micro Kit?
FAQ ID -428
What is the maximum amount of starting material that can be processed with the RNeasy Micro Kit?
FAQ ID -426
What is the minimum elution volume when using the RNeasy MinElute Cleanup Kit?
FAQ ID -432
What is the smallest sample size that can be used with RNeasy Mini Kits?
FAQ ID -485
What is a homogeneous LiquiChip assay?
FAQ ID -476
What detection reagents (reporter fluorophores) do you recommend for the LiquiChip System?
FAQ ID -480
What are the excitation and emission wavelengths of the reporter and classification lasers of the LiquiChip Instrument?
FAQ ID -479
What is the binding capacity of the RNeasy MinElute Cleanup Kit column?
FAQ ID -430
What is the composition and size of the LiquiChip Beads?
FAQ ID -475
What is the binding capacity of the RNeasy Micro Kit column?
FAQ ID -427
Is dihydrofolate reductase (DHFR) immunogenic?
FAQ ID -471
What are some of the assays that are adaptable to the LiquiChip System?
FAQ ID -477
My QuantiTect Master Mix did not freeze at degrees. Is it still ok to use?
-20
I want to start with gene silencing (RNAi) experiments. Do you have informational literature?
FAQ ID -580
Is it possible to use less than 1 mg of protein with the PhosphoProtein Purification Kit?
FAQ ID -599
Is it possible to use a QIAzol lysate for the isolation of RNA from tissue on the BioRobot EZ1?
FAQ ID -573
What is a quenched FRET assay?
FAQ ID -542
What do you recommend for isolating RNA from more than 100 mg of plant tissue?
FAQ ID -570
What happens if I spin my lysate on the RNeasy Spin Columns at maximum speed?
FAQ ID -514
How should I dissolve the lyophilized Anti-His and Tag Antibodies?
-100
Is it possible to perform a UNG treatment when using QuantiTect kits?
FAQ ID -564
Is it possible to clean up a methylation reaction containing bisulfite with QIAquick Cleanup Kits?
FAQ ID -519
Is it possible to isolate both RNA and recombinant 6xHis-tagged protein from the same sample?
FAQ ID -532
Why are my realtime PCR amplification plots hook-shaped?
FAQ ID -587
Why are samples centrifuged at 4°C after the addition of chloroform when using RNeasy Lipid Tissue Kits?
FAQ ID -533
How was it determined that the PhosphoSerine Antibody will bind phosphoserines only?
FAQ ID -579
If pQE40 is used to express a fusion protein with DHFR, can the DHFR be cleaved afterwards?
FAQ ID -513
Which chloroform should I use for the RNeasy Lipid Tissue Kit?
FAQ ID -516
Will pancreas tissue swim on top of RNAprotect Tissue Reagent?
FAQ ID -536
Will the 2x QIAGEN Multiplex PCR Master Mix freeze at -20°C?
FAQ ID - 525
Why does my realtime PCR assay quality decrease over time?
FAQ ID -589
What is the longest fragment that was cloned with the pQE UA Cloning Vector?
-30
What is the difference between BSA-free and BSA-containing RGS-His Antibody?
FAQ ID -569
When using QIAzol Lysis Reagent for RNA extraction, I see a pinkish colored aqueous phase! Is it OK?
FAQ ID -534
Where can I find the public Smith-Waterman homology search tool that you refer to on your siRNA online design page?
FAQ ID -562
What reaction volume should I use with QuantiTect Kits?
FAQ ID -551
What makes QIAGEN's 10x Taq and HotStarTaq DNA Polymerase PCR buffer superior?
FAQ ID -566
How many template copies can I detect using QuantiTect Kits in real-time PCR?
FAQ ID -543
Can I use RNAiFect for co-transfection of plasmid DNA and siRNA?
FAQ ID -515
Does DEPC harm RNA?
FAQ ID -529
Does endogenously expressed DAPase in human cells degrade proteins that are expressed in cell culture?
FAQ ID -527
Can the MAPK1 control siRNA be used for rat cells?
FAQ ID -547
Are the columns of the MinElute Reaction Cleanup-, Gel Extraction-, and PCR Purification Kit identical?
FAQ ID -581
After using your U/A PCR Cloning Kit I can detect colonies, which are light blue, and not really white. Why is that?
FAQ ID -512
Can I make a master mix with QuantiProbes, primers, and all other reaction components and store it for later use?
FAQ ID -584
Can I homogenize samples in QIAzol using the QIAshredder?
FAQ ID -501
Can I shorten the activation time for the HotStarTaq DNA Polymerase?
FAQ ID -565
Does QIAGEN sell 6xHis-tagged proteins besides the 6xHis Protein Ladder?
FAQ ID -594
Do you offer QIAshredder spin columns for the RNeasy Midi/Maxi Kit format? If not, what is the alternative?
FAQ ID -560
Do you provide control proteins for use with the PhosphoProtein Purification Kit?
FAQ ID -596
How long should the amplicon be if I am using QuantiTect SYBR Green detection?
FAQ ID -553
How is an antibody epitope determined?
FAQ ID -593
Do I need to calibrate my real-time cycler if I want to use Yakima Yellow?
FAQ ID -540
Do we recommend pooling siRNA sequences?
FAQ ID -517
Does the His-tag interfere with the purification procedure of the QIAGEN PhosphoProtein Purification Kit?
FAQ ID -598
Does the use of MAPK1 as a control in siRNA transfection experiments have secondary effects on the cell culture?
FAQ ID -548
Do you have a protocol to isolate RNA from blood and bone marrow using the BioRobot EZ1?
FAQ ID -567
Do you have any recommendations for using the PosphoProtein Kit with starting material other than cells?
FAQ ID -597
Do you have a protocol for isolating RNA from yeast using RNeasy?
FAQ ID -535
What is the run time for the BioSprint 96 DNA Blood protocol on the BioSprint 96 instrument?
FAQ ID -622
Will REPLI-g work at high temperatures?
FAQ ID -656
The Multiflow Dispense Head on my BioRobot is not dispensing the correct amount of liquid. What can I do?
FAQ ID -652
What is the smallest amount of DNA I can put into a 50 ul REPLI-g reaction?
FAQ ID -663
What is the recommended incubation time to stabilize tissue RNA in RNAprotect Tissue Reagent?
FAQ ID -647
What do you mean by “Quantification of locus representation” in REPLI-g WGA product?
FAQ ID -698
What is REPLI-g whole genome amplification?
FAQ ID -654
What is the average amount of DNA and RNA present in 1 ml normal serum?
FAQ ID -635
What is the biggest protein that can be synthesized with the EasyXpress Protein Synthesis System?
FAQ ID -601
What is the length of the MDA whole genome amplification product?
FAQ ID -690
What is the expected yield of genomic DNA isolated from bacteria with the DNeasy Blood & Tissue or QIAamp DNA Mini Kit?
FAQ ID -632
Is it possible to perform laser microdissection with tissues stabilized with RNAprotect Tissue reagent?
FAQ ID -608
Is it possible to synthesize membrane proteins using the EasyXpress Protein Synthesis Kit?
FAQ ID -602
What are Elution Microtube adapters and Microplate adapters used in the DirectPrep 96 Miniprep Kit, and how can I order them?
FAQ ID -646
What are the advantages of REPLI-g over conventional DNA sample processing and amplification methods?
FAQ ID -686
Why do I get amplification in a negative control DNA tube using the REPLI-g Kit for WGA?
FAQ ID -675
Why did you choose MAPK1 as a positive control in the QIAGEN RNAi Human/Mouse Control Kit?
FAQ ID -604
Will the PhosphoSerine antibody recognize Serine residues if the neighboring residues are Leucine or Isoleucine?
FAQ ID -644
Will TA cloning work with REPLI-g WGA product?
FAQ ID -672
Will DNA from chromosomes collected in TE with a drop of mineral oil work with REPLI-g amplification?
FAQ ID -664
What is the stability of the REPLI-g MDA product?
FAQ ID -693
When delivering liquid to a 96-well plate using the MultiFlow Dispenser Head on my BioRobot , one or more rows in the plate contains less liquid per well than specified by the protocol. What is going wrong?
FAQ ID -653
Where should I add a dye-label modification on the siRNA?
FAQ ID -648
What types of materials from environmental samples could interfere with WGA?
FAQ ID -666
Can I stabilize RNA in bacteria grown on solid media or substrate using the RNAprotect Bacteria Reagent?
FAQ ID -615
Can I re-amplify whole genome amplification product?
FAQ ID -670
Can I use another lysis buffer than the one supplied in the PhophoProtein Purification Kit?
FAQ ID -645
Can I run less than 15 samples on the BioSprint 15 instrument?
FAQ ID -621
Can the QIAshredder Maxi Spin Columns from the DNeasy Plant Maxi Kit be used for RNeasy samples?
FAQ ID -616
Do you have an off-the shelf diagnostics kit for WGA?
FAQ ID -678
Are there components in biological samples that can inhibit the REPLI-g reaction?
FAQ ID -667
Are the BioSprint 15/96 instruments stand-alone workstations?
FAQ ID -620
Can the computer for the LiquiChip Workstation be networked?
FAQ ID -629
Are QIAGEN EZ Competent Cells of the QIAGEN PCR Cloning Kit dam+ or dam-?
FAQ ID -638
Are samples that are contaminated with high molecular weight DNA (e.g. from a paramedic or police officer) a problem with REPLI-g amplification?
FAQ ID -683
Can BAC DNA prepared with the R.E.A.L. Prep 96 Plasmid Kit be used for high-throughput sequencing?
FAQ ID -643
Can I do whole genome amplification from brain tissue using REPLI-g?
FAQ ID -669
Can I do whole genome amplification from mitochondrial DNA using REPLI-g?
FAQ ID -668
Can FTA paper for dried blood spots be used with REPLI-g?
FAQ ID -685
How can I quantify the amount of REPLI-g DNA I have amplified?
FAQ ID -694
Can I amplify from plasma or serum samples using the REPLI-g Mini/Midi kits?
FAQ ID -671
How should I store the REPLI-g amplification product?
FAQ ID -692
Is 2 kb the minimal gDNA fragment size for REPLI-g Whole Genome Amplification?
FAQ ID -682
How long can I store bacterial culture stabilized in RNAprotect Bacteria Reagent?
FAQ ID -614
Do I need to clean up the REPLI-g amplification product for downstream analysis?
FAQ ID -691
Do you have a kit for RNA isolation from any kind of sample type?
FAQ ID -627
Does REPLI-g technology for whole genome amplification work with paraffin embedded samples?
FAQ ID -673
How can I determine the quality of my REPLI-g amplified products?
FAQ ID -695
How can I decrease ribosomal RNA (rRNA) contamination using Oligotex?
FAQ ID -642
Do you know of anything in the buffers/DNA sample that can cause bias in REPLI-g WGA?
FAQ ID -684
Do you have a protocol for the purification of DNA from rodent tail using the BioSprint System?
FAQ ID -979
Do you have a protocol for the isolation of DNA from epithelial cells mixed with sperm cells?
FAQ ID -926
Do you have a protocol for isolation of genomic DNA from saliva using the DNeasy Blood & Tissue Kit?
FAQ ID -931
Do you have a protocol for purification of DNA from clotted blood using the FlexiGene DNA Kit?
FAQ ID -973
Do you have a protocol for Streptokinase treatment of clotted blood?
FAQ ID -912
Do you have a protocol for the isolation of total nucleic acids from animal and human cells on the BioRobot EZ1?
FAQ ID -974
Do you have a protocol for the isolation of total RNA from plant tissue using QIAGEN-tips?
FAQ ID -949
Do you have a protocol for the isolation of total RNA from buccal swabs?
FAQ ID -956
Do you have a protocol for the isolation of viral DNA from stool?
FAQ ID -929
Do you have a protocol for the isolation of total RNA from plants using the RNeasy 96 Kit?
FAQ ID -951
Do you have a protocol for the isolation of DNA from formalin-preserved stool samples?
FAQ ID -922
Do you have a protocol for the isolation of genomic DNA from fungi?
FAQ ID -911
Do you have a protocol for the isolation of genomic DNA and/or proteins from fatty tissue treated with QIAzol?
FAQ ID -955
Do you have a protocol for the purification of DNA from cells using the BioSprint System?
FAQ ID -977
Do you have a protocol for the purification of DNA from dried blood spots using the BioSprint System?
FAQ ID -982
Do you have a protocol for the preparation of tissues preserved in RNAprotect Tissue Reagent for pathological studies?
FAQ ID -950
Do you have a protocol for the purification of DNA from buccal swabs using the BioSprint System?
FAQ ID -983
Do you have a protocol for isolation of genomic and viral DNA from lymphocytes using QIAGEN Genomic-tips?
FAQ ID -901
Do you have a protocol for isolation of genomic and plasmid DNA from cell cultures using QIAGEN Genomic-tips?
FAQ ID -902
Do you have a protocol for transfection of adherent cells with low concentrations of siRNA using RNAiFect Transfection Reagent?
FAQ ID -970
Do you have a protocol for GMO testing of food samples?
FAQ ID -921
Do you have a protocol for isolation of bacterial DNA from soil?
FAQ ID -923
Do you have a protocol for isolation of genomic DNA from dried blood spots using the QIAamp 96 DNA Blood Kit?
FAQ ID -919
Do you have a protocol for isolation of genomic DNA from insects?
FAQ ID -904
Do you have a protocol for detection of HBV DNA by PCR?
FAQ ID -915
Does QIAGEN sell a vector with a T7 promotor for use with the EasyXpress Protein Synthesis Insect Kit?
FAQ ID -937
Can the BioSprint instruments be placed in a cool room?
FAQ ID -938
Do you have a protocol for Acetyl Cysteine (NALC) treatment of viscous samples?
FAQ ID -913
Do you have a protocol for co-transfection of adherent cells with siRNA and plasmid DNA?
FAQ ID -969
Do you have a protocol for adding new reporter dyes to the ABI PRISM 7900 Sequence Detection System?
FAQ ID -965
Do you have a protocol for cleanup of non-radioactive DNA samples?
FAQ ID -945
Do you have a protocol for radioactive labelling of cDNA?
FAQ ID -960
Do you have protocols for multiple extractions of DNA fragments from agarose gels?
FAQ ID -944
Why is it not recommended to stabilize cells with RNAprotect Tissue Reagent?
FAQ ID -941
Do you have a protocol for the detection of Bordetella pertussis DNA by PCR?
FAQ ID -914
Do you have a protocol for the isolation of DNA from cultured cells using the BioRobot EZ1?
FAQ ID -990
Do you have a protocol for performing quantitative multiplex PCR on the LightCycler 2.0 system?
FAQ ID -966
Do you have a protocol for long-term, siRNA-mediated gene silencing using HiPerFect Transfection Reagent?
FAQ ID -972
Do you have a protocol for manual purification of 6xHis-tagged proteins expressed in E. coli using Ni-NTA Superflow?
FAQ ID -967
Do you have a protocol for purification of DNA from cultured animal cells using the DNeasy 96 Blood & Tissue Kit?
FAQ ID -934
Do you have a protocol for purifying 2x96 PCR samples simultaneously with the QIAquick 96 PCR Purification Kit?
FAQ ID -935
Do you have a protocol for purification of DNA fragments from Cy3/Cy5 dye-label reactions?
FAQ ID -947
Do you have a protocol for polyacrylamide gel analysis of oligonucleotides?
FAQ ID -961
Do you have a protocol for isolation of genomic DNA from plants and filamentous fungi using QIAGEN Genomic-tips?
FAQ ID -905
Do you have a protocol for isolation of genomic DNA from flies using QIAGEN Genomic-tips?
FAQ ID -903
What are exo-resistant random hexamers used in the REPLI-g reaction?
FAQ ID -710
The Sigma Centrifuge makes a loud noise at low speeds which disappears at higher speeds, when using the 2x96-well plate rotor. What is the problem?
FAQ ID -776
Is RNAprotect Bacteria Reagent compatible with the RNeasy Mini Kit?
FAQ ID -797
What is the basic technology behind the Strep-tag Protein Purification System?
FAQ ID -740
What are the expected average DNA yields from plant extractions on the BioSprint 96?
FAQ ID -771
If template abundance differs significantly between housekeeping and target gene, are both amplified equally efficient with the QuantiTect Multiplex Kits?
FAQ ID -718
How to quantitate DNA purified using the BioSprint 96?
FAQ ID -773
How to store DNA from forensic samples eluted into water on the EZ1 biorobot in TE buffer instead?
FAQ ID -734
Is it possible to use LiquiChip beads for FACS applications?
FAQ ID -733
Is it possible to buy the EZ Competent Cells of the QIAGEN PCR Cloning Plus Kit separately?
FAQ ID -763
Is GFP excited and detected by the green laser of the LiquiChip System?
FAQ ID -737
Which Qproteome or Protein Fractionation Kits from QIAGEN are compatible with tissue samples?
FAQ ID -755
Why are some of my RNAprotect-stabilized samples frozen at 0ºC while others are not?
FAQ ID -758
Why are the QuantiTect and QuantiFast Multiplex PCR Kits limited to triplex real-time PCR on some cyclers?
FAQ ID -715
Why should DNA or cDNA targets be less than 250 bp long for real-time PCR?
FAQ ID-751
Where can I find info on compatible reporter dyes for use in real-time multiplex PCR using the QuantiTect Multiplex PCR Kits?
FAQ ID -719
Why might my gene of interest drop out during WGA if it is not near a telomere or centromere?
FAQ ID -703
Will the random hexamers in the REPLI-g reaction interfere with downstream analysis?
FAQ ID -713
Why is there no detection of free reporter fluorescence in the LiquiChip System?
FAQ ID -732
What is the cell number range one can use with the FastLane Cell cDNA Kit for real-time RT-PCR?
FAQ ID -782
What negative (unphosphorylated) and positive (phosphorylated) control proteins do you recommend for use with the PhosphoProtein Purification Kit?
FAQ ID -779
What is the enzyme used in the REPLI-g reaction?
FAQ ID -704
What type of Sequencing Services does QIAGEN offer?
FAQ ID -781
Where can I find a copy of the report files generated by running accessory protocols "Dilutor Delivery (monthly)" and "High-Speed Pipetting Sytem (monthly)" on my BioRobot 9604?
FAQ ID -766
What is the general sensitivity level of the LiquiChip System?
FAQ ID -731
Can QIAGEN's Taq- and HotstarTaq DNA Polymerases be used for cycle sequencing?
FAQ ID -741
Can I use the PhosphoProtein Purification Kit to purify phosphorylated peptides of around 1 kD size?
FAQ ID -778
Can RNAprotect Tissue Reagent be used to stabilize tissue which has already been flash-frozen?
FAQ ID -757
Can lyophilized plant material be used for DNA extraction on the BioSprint 96?
FAQ ID -772
Can the REPLI-g WGA enzyme work with labeled dNTPs?
FAQ ID -706
Can the MAPK1 siRNA of the RNAi Human/Mouse Starter Kit be purchased separately?
FAQ ID -744
Can the QIAvac 96 vacuum manifold be used with DirectPrep 96 plates?
FAQ ID -722
Are the buffers in the Ni-NTA Fast Start Kit the same as the ones for use with Ni-NTA purchased separately?
FAQ ID -791
Any data on the fidelity of the REPLI-g MDA technique?
FAQ ID -707
Are Centromeres and Telomeres amplified using REPLI-g WGA?
FAQ ID -702
Can I use my own primers for REPLI-g WGA to amplify a specific chromosomal region?
FAQ ID -712
Can I do 4-plex real-time PCR, on the ABI PRISM 7000, 7700, or 7900 using the QuantiTect Multiplex PCR Kit?
FAQ ID -716
How can one perform multiplex real-time PCR analysis using the LightCycler 2.0?
FAQ ID -780
How can I be sure that I am harvesting my induced bacterial culture at the best time point for protein expression?
FAQ ID -788
How do the FastLane Cell cDNA Kit and QuantiTect Reverse Transcription Kit eliminate genomic DNA contamination?
FAQ ID -783
How many cells can be used with the FastLane Cell cDNA Kit?
FAQ ID -796
Do you have an antibody for Western Blot Analysis of MAPK1 gene knockdown using your MAPK1 control siRNA?
FAQ ID -746
Can frozen blood be used for whole genome amplification with REPLI-g?
FAQ ID -794
Do I have to switch off the laser of the LiquiChip Workstation overnight?
FAQ ID -736
How many reporter dyes can be used in the same analysis using the LiquiChip Workstation?
FAQ ID -738
Do I need to determine limiting primer concentrations with the QuantiTect Multiplex PCR Kits?
FAQ ID -714
Do you synthesize thiophosphate modified random hexamers?
FAQ ID -711
Has anyone verified whole genome amplification accuracy with Sequencing?
FAQ ID -701
Do you have a protocol for the fixation of cells transfected with fluorescently labeled siRNA?
FAQ ID -793
How best to handle the light sensitive LiquiChip reagents?
FAQ ID -735
Do you have information on the use of recombinant DNA and RNA as absolute standards for realtime RT-PCR?
FAQ ID -729
Is it possible to transfect DNA into insect cell lines such as Drosophila melanogaster S2?
FAQ ID -397
Is it possible to isolate free genomic DNA using the QIAamp UltraSens Virus Kit?
FAQ ID -358
Is it possible to store TAGzyme enzymes at°C?
-80
I accidentally added RNAprotect Tissue Reagent to my cells, and now the cells are difficult to pellet. What can I do?
FAQ ID -364
What are the calculated molecular weights of the TAGZyme enzymes?
FAQ ID -329
How complete is the removal of DAPase in the TAGZyme process?
FAQ ID -319
How long is fluorescence detectable in cells after transfection with fluorescently labeled siRNAs?
FAQ ID -392
How do I submit a siRNA order by telephone or online?
FAQ ID -399
How many transfections can I perform with 20 nmol of HPP grade siRNA?
FAQ ID -367
Which primers can I use for sequencing pQE-expression vector constructs?
FAQ ID -343
What is the standard assay volume recommended for LiquiChip assays?
FAQ ID -330
What is the acceptable maximum g force for centrifugation of Xa Removal Resin?
FAQ ID -320
What is the lowest elution volume that can be used with QIAprecipitator Midi and Maxi Modules?
FAQ ID -307
What is the composition of Buffer FG3?
FAQ ID -356
Can I use ethanol instead of isopropanol for DNA precipitation when using HiSpeed Plasmid Kits?
FAQ ID -354
303 - Can I use my own lysis buffer with the DNeasy Blood & Tissue or QIAamp DNA Mini Kit?
Can I use a partial MinElute 96 UF plate?
FAQ ID -355
Can I use the RNeasy Mini Kit or RNeasy 96 Kit with fewer than 100 cells?
FAQ ID -372
Can I use water for elution from the QIAprecipitator of the HiSpeed Plasmid Kits?
FAQ ID -306
Are the QIAprecipitator Midi and Maxi Modules of the HiSpeed Plasmid Kits interchangeable?
FAQ ID -308
Apart from the antibody itself, does the Penta-His Biotin Conjugate solution contain any protein?
FAQ ID -359
Are QIAGEN monoclonal Anti His Antibodies glycosylated?
FAQ ID -334
Can I perform in-vitro transcription with the pDrive Cloning Vector from the QIAGEN PCR Cloning Kit?
FAQ ID -332
Do the QuantiTect SYBR Green Kits contain a stabilization reagent that inhibits the sensitivity of real-time PCR?
FAQ ID -328
Do I need to anneal, deprotect or desalt my QIAGEN siRNA?
FAQ ID -398
How are siRNAs introduced into C. elegans?
FAQ ID -396
Do pQE vectors contain the CAT gene?
FAQ ID -362
How can I improve the expression of proteins containing hydrophobic regions?
FAQ ID -339
How can I check if any residual proteins remain on the Ni-NTA Agarose matrix after elution?
FAQ ID -324
Do freeze-thaw cycles affect the stability of the U-overhang on the pDrive Cloning Vector?
FAQ ID -323
Can RNAprotect Tissue Reagent be used to store previously isolated RNA?
FAQ ID -337
Can MinElute 96 UF PCR be used to purify PCR samples which contain mineral oil?
FAQ ID -360
Does SYBR Green dye present in QuantiTect kits interfere with ethidium bromide staining?
FAQ ID -312
Do any of the buffers in the HotStarTaq DNA Polymerase Kit contain Triton?
FAQ ID -327
Do any of the kit components or the product packaging of the QIAamp 96 DNA Blood Kit contain latex?
FAQ ID -318
What data documentation does QIAGEN's Sequencing Service provide for customers?
FAQ ID -832
What are the sequences of the FlexiTube siRNAs?
FAQ ID -851
My BioRobot 9604 came with several Accessory Applications. Is it recommended I run these protocols?
FAQ ID -872
What is the proper way to turn on or turn off the EZ1 instrument?
FAQ ID -870
What is the Energy Mix in the EasyXpress Kits?
FAQ ID -846
What is the concentration of the primers in a reaction using the QuantiTect Primer Assays?
FAQ ID -850
I would like to sequence my siRNA constructs. Does QIAGEN offer Sequencing Services for cloned siRNA?
FAQ ID -829
Is it possible to use frozen cell pellets with the FastLane Cell cDNA Kit?
FAQ ID -835
Is it possible to elute plasmid DNA from the QIAprep Spin Miniprep columns with buffer containing Potassium Phosphate?
FAQ ID -854
Is it possible to perform immunoprecipitation (IP) directly on protein fractions from the Qproteome Cell Compartment Kit?
FAQ ID -855
Is it possible to adjust the Lysis Buffer volume used with the Qproteome Bacterial Protein Preparation Kit?
FAQ ID -841
Is it possible to use QIAGEN's pQE-TriSystem Vectors with the EasyXpress Protein Synthesis Insect Kit?
FAQ ID -876
Is there a stopping point in the protocol for Suspended Cells using the FastLane Cell cDNA Kit?
FAQ ID -837
Is the random biotin labeling of proteins expressed with your EasyXpress Biotin Labeling Kit as sensitive as labeling with radioactivity?
FAQ ID -875
Will biotin detection assays be efficient when doing in vitro translation from E. coli lysates with the EasyXpress Site-Specific Biotin Kit?
FAQ ID -878
Why would clumps occur following the addition of Buffer P2 when using LyseBlue Reagent in a plasmid preparation?
FAQ ID -862
Will acetone precipitation recommended in the Qproteome Protocols denature Protein?
FAQ ID -807
Will site-specific biotin labeling with the EasyXpress Site-Specific Biotin kit alter the structure and function of my protein?
FAQ ID -877
Will the sequences of the QuantiTect Primer Assays be provided?
FAQ ID -804
Will the FastLane Cell cDNA Kit work with suspension cells?
FAQ ID -801
Which resin is used in the QIAexpress Ni-NTA Fast Start Columns?
FAQ ID -836
What publicly funded Genome Sequencing Projects has QIAGEN's Sequencing Service been involved in?
FAQ ID -815
What is the typical yield of site-specific labeled protein generated using the EasyXpress Site-Specific Biotin Kit?
FAQ ID -873
What programs does your Sequencing Service use to ensure accurate base calling, Sequence Quality, and Alignment?
FAQ ID -831
Where can I find a copy of the report file generated by running 'Maintenance Protocol I' on my BioRobot MDx?
FAQ ID -806
Which fraction will contain soluble mitochondrial proteins using the Qproteome Cell Compartment Kit?
FAQ ID -839
Which fraction will contain endosomal, microsomal and lysosomal proteins using the Qproteome Cell Compartment Kit?
FAQ ID -808
Where can I find QIAGEN products for a specific gene or gene product?
FAQ ID -803
Can the QuantiTect Primer Assays be used for detection of genomic DNA?
FAQ ID -811
Do I need to use RNase inhibitors with the RNeasy Kits?
FAQ ID -813
Do the Buffers CE1 - CE4 of the Qproteome Cell Compartment Kit contain DTT or any reducing agent?
FAQ ID -824
Can the FastLane Cell cDNA Kit also be used for qualitative end-point PCR?
FAQ ID -834
Can the FastLane Cell cDNA Kit be used with tissue?
FAQ ID -821
Do you have a protocol for the isolation of bacteriophage P1 derived constructs with any of your plasmid kits?
FAQ ID -883
Do you have a protocol for the isolation of BAC DNA using the QIAGEN Plasmid Midi Kit?
FAQ ID -881
895 - Do you have a protocol for the isolation of endotoxin-free plasmid DNA using the QIAGEN Plasmid Midi Kit?
Do you have a protocol for isolation of plasmid DNA using the Sigma Centrifuge 4?
-15
Can Buffer R1 of the R.E.A.L. Prep 96 Plasmid Kit be replaced with Buffer P1?
FAQ ID -823
Are there specific recommendations for performing RT-PCR on RNA isolated from paraffin-embedded samples?
FAQ ID -828
Can I stabilize adipose tissue using RNAprotect Tissue Reagent prior to RNA isolation?
FAQ ID -856
Can I use the PhosphoProtein Purification Kit for the isolation of phosphorylated proteins from yeast?
FAQ ID -838
Can I use QIAGEN's pQE vectors as templates in the EasyXpress Biotin Labeling Kits?
FAQ ID -874
Can I use my gene-specific primers with the FastLane Cell cDNA Kit and the QuantiTect Reverse Transcription Kit?
FAQ ID -812
Can I use LyseBlue with R.E.A.L. Prep 96, QIAwell Ultra, or QIAprep 96 Turbo Miniprep Kits for the BioRobot systems?
FAQ ID -863
Do you have information on in vivo RNA interference experiments?
FAQ ID -817
Do you sell the ATP-Dependent Exonuclease of the QIAGEN Large-Construct Kit separately?
FAQ ID -825
I cannot see the LCD display fonts on the Sigma Centrifuge. How do I adjust the contrast?
FAQ ID -822
How long does a typical in vitro translation reaction take with your large-scale EasyXpress protein synthesis kits?
FAQ ID -880
How much protein can be produced with the EasyXpress Protein Synthesis Mega Kit?
FAQ ID -843
Do you have a protocol for the isolation of very low-copy plasmids from Streptomyces spp.?
FAQ ID -893
Do you have a protocol for the isolation of plasmid DNA from Citrobacter freundii?
FAQ ID -885
Do you have a protocol for the isolation of plasmid DNA from Corynebacterium glutamicum?
FAQ ID -886
Do you have a protocol for the isolation of plasmid DNA from Borrelia spp.?
FAQ ID -884
Do you have a protocol for the isolation of plasmid DNA from Lactobacillus spp.?
FAQ ID -887
Do you have a protocol for the isolation of plasmid DNA from yeast?
FAQ ID -853
Do you have a protocol for the isolation of single-stranded DNA from M13 phage using QIAGEN Plasmid Kits?
FAQ ID -894
Do you have a protocol for the isolation of plasmid DNA from Staphylococcus spp.?
FAQ ID -890
Do you have a protocol for the isolation of plasmid DNA from Oligotropha carboxidovorans?
FAQ ID -888
Do you have a protocol for the isolation of plasmid DNA from Proteus spp.?
FAQ ID -889
What are your recommendations for cotransfecting several plasmids?
FAQ ID -124
What are the recommended culture and buffer volumes for a very low-copy plasmid?
FAQ ID -168
Is mRNA isolation necessary for sensitive RT-PCR?
FAQ ID -111
When should carrier be used with the QIAamp DNA Mini or the DNeasy Blood & Tissue Kit?
FAQ ID -100
Which Anti-His Antibody is the most sensitive for my protein of interest?
FAQ ID -172
Why do I have to add beta-mercaptoethanol (beta-ME) to lysis Buffer RLT of the RNeasy Kits?
FAQ ID -101
What is the recommended culture medium for the QIAprep System?
FAQ ID -154
How can I eliminate contaminating protein in my Ni-NTA 6xHis-tag protein purification?
FAQ ID -102
How can I purify very small amounts of 6xHis-tagged protein using Ni-NTA technology?
FAQ ID -134
How can I improve ligation efficiency of DNA from a QIAEX II Gel Extraction Kit?
FAQ ID -141
How can I isolate RNA from 1 gram of plant tissue?
FAQ ID -128
How can I improve the performance of the HiSpeed QIAprecipitator module?
FAQ ID -144
What can I use to isolate RNA smaller than 200 nucleotides?
FAQ ID -115
What epitopes do the Anti-His Conjugates and Anti-His Antibodies recognize?
FAQ ID -170
What are the maximum culture volumes to use with the QIAGEN Plasmid Midi or Maxi Kit?
FAQ ID -167
How should RNeasy Kits be stored and how long are they stable?
FAQ ID -103
Is it possible to cleave the 6xHis-tag from an expressed protein?
FAQ ID -140
How should QIAGEN Plasmid Purification Kits be stored and for how long?
FAQ ID -192
What are the features and benefits of the QIAexpress 6xHis Tag System?
FAQ ID -193
Do you have transfection data for QIAGEN Transfection Reagents?
FAQ ID -158
Do you have data showing effects of sample size on DNA yield and purity using the DNeasy 96 Blood & Tissue kit?
FAQ ID -129
Does QIAGEN offer vectors for direct cloning of PCR products?
FAQ ID -146
How can I avoid variable transfection efficiencies with SuperFect Reagent?
FAQ ID -179
Can DyeEx be used to clean up labeled DNA other than sequencing templates?
FAQ ID -137
Are QIAamp DNA isolation kits suitable for apoptosis studies?
FAQ ID -149
Can I use a QIAfilter Cartridge for purifying large plasmids?
FAQ ID -121
Can I use Omniscript or Sensiscript RT's at a higher temperature?
FAQ ID -116
How do you ensure that RNeasy buffers are RNase-free?
FAQ ID -113
How can I purify DNA from soil, food and sewage samples for PCR?
FAQ ID -118
How should I store Anti·His and Tag·100 Antibodies?
FAQ ID -194
How do I prepare an insert for pQE vectors?
FAQ ID -185
How can I obtain DNA-free RNA using an RNeasy Midi or Maxi Kit?
FAQ ID -143
What are the compatibilities of different reagents with Ni-NTA matrices?
FAQ ID -49
How can I prevent running out of M48 Kit reagents before the full number of samples are processed?
FAQ ID -1042
Do you have a protocol for the isolation of genomic DNA from paraffin-embedded samples on the BioRobot M48?
FAQ ID -1019
Is the TurboCapture Microplate Holder Type B compatible with the Biomek FX robotic workstation?
FAQ ID -1040
Is there a limit to the size of proteins that can be purified using the Qproteome Glycoprotein Kits?
FAQ ID -1071
Is it possible to scale up QuantiTect Reverse Transcription reactions to allow use of larger amounts of RNA?
FAQ ID -1063
Is it possible to use the PhosphoProtein Purification Kit in batch format?
FAQ ID -1069
What are the recommended storage conditions for the QuantiTect Reverse Transcription Kit and its components?
FAQ ID -1077
What is the difference between TurboCapture Microplate Holders Type A and Type B?
FAQ ID -1038
What blocking reagents do you recommend for chemiluminescent detection using Penta-, Tetra-, and RGS-His antibodies?
FAQ ID -1093
Is Factor SB of the HotStar HiFidelity Polymerase Kit the same as Factor MP of the QIAGEN Multiplex PCR Kit?
FAQ ID -1049
How can I transfect siRNA into insect cells such as Drosophila melanogaster S2?
FAQ ID -1046
How can I tell TurboCapture Microplate Holder Type A from Type B?
FAQ ID -1039
How much template can I use in the reaction and what is the maximum volume of template that can be used in the QuantiTect Kits?
FAQ ID -1086
How much dye-labeled siRNA should I use to monitor transfection efficiency when using HiPerfect Transfection Reagent?
FAQ ID -1058
What is the error rate of HotStar HiFidelity DNA Polymerase?
FAQ ID -1048
Which QIAGEN kit do you recommend for purifying plasmid DNA suitable for transfection of sensitive cells?
FAQ ID -1092
Which kit should I use for RNA isolation from Cartilage?
FAQ ID -1026
Where can I find the serial number on QIAGEN's BioRobots?
FAQ ID -1064
Which kit can be used for simultaneous purification of DNA and RNA from biopsies?
FAQ ID -1089
Why is there RNA in the DNA eluate when using the AllPrep DNA Mini Spin Column?
FAQ ID -1044
Why does my PCR product show up later when comparing the QuantiTect SYBR Green PCR Kits with Roche kits using the same annealing temperature?
FAQ ID -1083
Will the "classic" RNeasy Mini Kit be discontinued after the launch of the Allprep DNA/RNA Mini Kit?
FAQ ID -1054
Why does TA/UA cloning work with your proofreading HotStar HiFidelity DNA Polymerase?
FAQ ID -1053
Why do melting temperatures differ between PCR fragments amplified with QIAGEN's QuantiTect SYBR Green PCR Kits and Roche Kits?
FAQ ID -1084
What is the range of melting temperatures for the QuantiTect Primer Assay amplicons in the dissociation curve?
FAQ ID -1079
What is the protein loading capacity per column of the Qproteome Albumin/IgG Depletion Kit?
FAQ ID -1090
What is your recommendation for cleaning the TissueLyser Bead Dispenser (96-well format)?
FAQ ID -1055
What is the resin in the Qproteome Murine Albumin and Qproteome Albumin/IgG Depletion Kits?
FAQ ID -1034
How can I prevent clogging of QIAfilter cartridges?
FAQ ID -1060
What is the volume of the eluate when using either spin or vacuum procedures with the QIAprep 96 Turbo Miniprep Kit?
FAQ ID -1067
Do I have to use a ramp time of 2°C/sec on the LightCycler when using Quantitect Primer Assays?
FAQ ID -1065
Does an activation time of 15 minutes influence the performance of the HotStarTaq Plus DNA Polymerase?
FAQ ID -1050
Do you have a protocol for the isolation of RNA from bacterial cultures using RNAprotect Bacteria Reagent and QIAzol Lysis Reagent?
FAQ ID -1020
Do you have a protocol for the isolation of DNA from mouse tails using MagAttract technology on BioRobot M96?
FAQ ID -1007
Do you have a protocol for the isolation of DNA from forensic samples using the BioRobot M48?
FAQ ID -1017
Can the USB Port of the BioSprint 96 instrument be used to transfer programs from a PC to the instrument?
FAQ ID -1032
Are your FlexiTube siRNAs preferentially designed to any particular region of the target gene?
FAQ ID -1091
Can PCR fragments generated with the EasyXpress Linear Template Kit be cloned into the pIX4.0 vector of the EasyXpress Protein Synthesis Insect Kit?
FAQ ID -1078
Can the QProteome O-Glycan Glycoprotein Kits be used for blood cells?
FAQ ID -1036
Can the Reverse Transcriptases of the QuantiTect Reverse Transcription Kit and the QuantiTect Probe RT-PCR Kit be used interchangeably?
FAQ ID -1066
Can the AllPrep RNA/Protein Kit be used with tissue?
FAQ ID -1095
Can the Blocking Reagent supplied with the Penta-His HRP Conjugate be used with other antibodies?
FAQ ID -1073
How can air bubbles in PhosphoProtein Purification Columns be avoided?
FAQ ID -1070
How can I increase low-copy plasmid DNA yields using QIAfilter Plasmid Kits?
FAQ ID -1062
How can I ensure complete genomic DNA removal when using the RNase-Free DNase Set?
FAQ ID -1087
Do you have information on the reproducibility of DNA yields from blood using the M48 BioRobot?
FAQ ID - 1004
Do you have a protocol for transient transfection of HEK 293 cells in 75 cm3 flasks?
FAQ ID -1014
Do you have a protocol for transient transfection of cells in 96-well plates using PolyFect Transfection Reagent?
FAQ ID -1013
Do you have a protocol for transient transfection of HeLa-S3 cells in 60 mm dishes using PolyFect?
FAQ ID -1012
Do you have a protocol for the isolation of total nucleic acids from cells on the M48 BioRobot?
FAQ ID -1009
Do you have a protocol for vacuum processing of blood samples with the QIAamp DNA Blood Midi or Maxi Kits?
FAQ ID -1011
Do you have a protocol for the isolation of total nucleic acids from tissue on the M48 BioRobot?
FAQ ID -1008
Do you have information on realtime RT-PCR performance of RNA isolated from monocytes on the M48 BioRobot?
FAQ ID -1010
Do you have information on DNA yields from blood with variable numbers of white blood cells using the M48 BioRobot?
FAQ ID -1006
Do you have information on PCR template preparation from preserved blood samples on the M48 BioRobot?
FAQ ID - 1003
Can the PAXgene Blood RNA Tubes undergo freeze/thaw cycles?
FAQ ID -2471
If I want to use BioMag particles, do I have to buy a QIAGEN magnet too or can I use one that I already have?
FAQ ID -266
Should I use Omniscript or Sensiscript for reverse transcription of low-copy mRNA?
FAQ ID -297
What are the main differences between other magnetic beads and the BioMag particles?
FAQ ID -272
My BioMag is about to expire, is it still any good?
FAQ ID -268
How magnetically responsive is BioMag?
FAQ ID -265
If I am using BioMag Streptavidin to capture biotinylated oligos, how can I then remove the oligos?
FAQ ID -279
Why is an ice-incubation step included during reaction set-up when following the QuantiTect RT-PCR but not the QuantiTect PCR protocol.
FAQ ID -283
What sequencing primers can I use with the pDrive cloning vector of the QIAGEN PCR Cloning Kit?
FAQ ID -292
What is the difference between the QIAamp MinElute Virus Spin and the MinElute Virus Vacuum Kit?
FAQ ID -295
What is the difference between the Fc specific versions of your secondary antibody BioMag particles and the non-specific?
FAQ ID -275
Can I use my own cycling conditions with the QIAGEN Multiplex PCR Kit?
FAQ ID -287
Can I select FITC labeled cells with BioMag?
FAQ ID -278
Can I use BioMag particles with any specific primary antibody?
FAQ ID -273
Are BioMag particles stable in other solvents?
FAQ ID -267
Can BioMag be used for positive and negative selection?
FAQ ID -269
How can I avoid poor immunolocalization morphology with Anti-His Antibodies?
FAQ ID -200
How can I prevent non-specific binding when using BioMag?
FAQ ID -277
How do I prevent bubbles from forming in my Ni-NTA agarose column?
FAQ ID -285
How can I purify mRNA from total RNA using an Oligotex Direct mRNA Kit?
FAQ ID -201
Is your Nuclease-Free Water fluorescence-free?
FAQ ID -1291
What quality level does your Nuclease-Free Water have?
FAQ ID -1292
How should the GelPilot DNA Loading Dye be handled?
FAQ ID -1287
Does GelPilot DNA Loading Dye contain ethidium bromide?
FAQ ID -1286
Can phenol/chloroform be placed in the MaXtract tube for extraction at a later time?
FAQ ID -1299
Are MaXtract tubes siliconized?
FAQ ID -1298
Should I input the dilution factor of my samples into the LiquiChip Analyser Software dilution factors of samples?
FAQ ID -1211
What cell lines have been tested with the Qproteome Plasma Membrane Protein Kit?
FAQ ID -1205
Is a saliva collection device provided in the RNeasy Protect Saliva Mini Kit?
FAQ ID -1213
Is it possible to increase nucleic acid concentrations when following protocols on the BioRobot EZ1?
FAQ ID -1232
Is RNAprotect Cell Reagent provided in the RNeasy Protect Cell Kit available separately?
FAQ ID -1217
Is it possible to use QIAGEN's pQE-Trisystem Vector with the EasyXpress Insect Kit II?
FAQ ID -1225
What vector do you recommend as template for in-vitro protein expression with the EasyXpress Insect Kit II?
FAQ ID -1224
What type of promoter should my template have for in-vitro protein expression with the EasyXpress Insect Kit II?
FAQ ID -1223
What is the maximum number of cells that can be used with the AllPrep RNA/Protein Kit ?
FAQ ID -1208
Will the Qproteome Plasma Membrane Protein Kit work with starting materials other than adherent mammalian cells?
FAQ ID -1202
What is the difference between various FlexiTube siRNAs listed for the same target gene, and which one should I choose?
FAQ ID -1206
What is the protein yield when using the Qproteome Plasma Membrane Protein Kit?
FAQ ID -1201
What is the size of the BioRobot EZ1?
FAQ ID -1245
Does anything have to be added to express post-translational modifications (PTMs) with the EasyXpress Insect Kit II?
FAQ ID -1226
Do you have a protocol for isolating cytoplasmic RNA from animal cells using the RNeasy Mini Kit?
FAQ ID -1207
Do you have a protocol for purification of miRNA from animal cells using the RNeasy Plus Mini Kit and RNeasy MinElute Cleanup Kit?
FAQ ID -1258
Can cells stabilized with RNAprotect Cell Reagent be used for cell sorting by flow cytometry (FACS)?
FAQ ID -1216
Can glycosylated proteins be expressed using the EasyXpress Insect Kit II?
FAQ ID -1227
Can RNAprotect Saliva Reagent be purchased separately?
FAQ ID -1214
Can you recommend a Reagent or Kit for stabilizing RNA in cultured cells?
FAQ ID -1215
Can the Strep-Tactin Magnetic Beads of the Qproteome Plasma Membrane Protein Kit be reused?
FAQ ID -1204
Can the Qproteome Plasma Membrane Protein Kit be used with tissue samples?
FAQ ID -1200
Can the RLT lysate of the BioSprint DNA Plant protocol be stored frozen?
FAQ ID -1248
How can I decontaminate the BioRobot EZ1?
FAQ ID -1231
How do you ensure that concentrated RNA is purified from saliva using the RNeasy Protect Saliva Mini Kit?
FAQ ID -1212
How much active protein can be expressed in-vitro using the EasyXpress Insect Kit II, and how much time does it take?
FAQ ID -1222
How many freeze-thaw cycles can the various reaction mixes in the EasyXpress Insect Kit II tolerate?
FAQ ID -1219
Do you have a protocol for the isolation of DNA from buccal cells using the BioRobot EZ1?
FAQ ID -1260
Do you have a protocol for transfection of differentiated macrophage cell lines (THP) with siRNA?
-1
How are plasma membrane containing vesicles eluted off the ligand in the Qproteome Plasma Membrane Protein procedure?
FAQ ID -1203
Which fixation method is used in the PAXgene Tissue System?
FAQ ID - 3600
What is the fixation reagent of PAXgene Tissue FIX?
FAQ ID - 3601
What is the stabilization reagent of PAXgene Tissue STABILIZER?
FAQ ID - 3602
What is the maximum tissue size that can be fixed in a PAXgene Tissue FIX Container (50 ml)?
FAQ ID - 3604
Why are two reagents used in the PAXgene Tissue System?
FAQ ID - 3603
Which conditions are recommended for the storage of tissues in PAXgene Tissue STABILIZER?
FAQ ID - 3605
Is it possible to process formalin-fixed and PAXgene Tissue fixed samples together in one run?
FAQ ID - 3606
Is it necessary to clean a processor normally used for formalin-fixed tissue before using it with PAXgene Tissue fixed tissue?
FAQ ID - 3607
Is it possible to integrate the PAXgene Tissue STABILIZER into tissue processing?
FAQ ID - 3608
Are immunohistochemistry (IHC) assays developed for formalin-fixed, paraffin-embedded (FFPE) tissues compatible with PAXgene Tissue fixed, paraffin-embedded (PFPE) tissues?
FAQ ID - 3609
How well is RNA integrity preserved in PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID - 3611
Is it possible to use formalin-fixed, paraffin-embedded (FFPE) kits and protocols to isolate biomolecules from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID - 3610
How well is DNA integrity preserved in PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID - 3612
Are special kits and protocols required to isolate biomolecules from PAXgene Tissue fixed, cryo-embedded (PFCE) tissue?
FAQ ID - 3614
What are the yield and integrity of nucleic acids isolated from blocks of PAXgene Tissue fixed, cryo-embedded (PFCE) tissue?
FAQ ID - 3613
Can proteins be extracted from PAXgene Tissue fixed specimens?
FAQ ID - 3616
Does the PAXgene Tissue RNA Kit co-purify small RNAs?
FAQ ID - 3615
Which kits and protocols can be used to isolate nucleic acids from microdissected PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) specimens?
FAQ ID - 3617
What is the PCR performance of DNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissue compared to DNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?
FAQ ID - 3618
How is the quality of proteins purified from PAXgene Tissue fixed and stabilized tissue?
FAQ ID - 3619
Can the RNeasy kits be used to extract RNA from saliva collected using the Oragene collection kit?
FAQ ID - 3623
How do I analyze codon 61 mutations with the RAS extension kit (cat. no. 971590)?
FAQ ID - 3622
Are QR codes recognized by QIAsymphony?
FAQ ID - 3625
Do I need to use a blood collection set with the PAXgene Blood ccfDNA Tube?
FAQ ID - 3626
What is the reagent in the PAXgene Blood ccfDNA Tube?
FAQ ID - 3632
Does the insert sequence provided in the specification sheet correspond to the target sequence or to anti-sense sequence?
FAQ ID - 3627
What is the shelf life of unused PAXgene Blood ccfDNA Tubes?
FAQ ID - 3633
How should unused PAXgene Blood ccfDNA Tubes be stored?
FAQ ID - 3634
Are there special considerations for phlebotomy when using the PAXgene Blood ccfDNA Tube (blood collection set, discard tube, positioning of the tube, issues with short draws, etc.)?
FAQ ID - 3635
Are PAXgene Blood ccfDNA Tubes sterile?
FAQ ID - 3636
What is the expected ccfDNA yield from plasma generated from 10 ml of blood?
FAQ ID - 3637
Can I use plasma from underfilled PAXgene Blood ccfDNA Tubes?
FAQ ID - 3638
How long can whole blood stabilized in PAXgene Blood ccfDNA Tubes be stored at room temperature?
FAQ ID - 3639
Can whole blood in PAXgene Blood ccfDNA Tubes be stored at temperatures above 25°C?
FAQ ID - 3640
Can whole blood in PAXgene Blood ccfDNA Tubes be stored at temperatures below 15°C?
FAQ ID - 3641
Can whole blood be frozen in PAXgene Blood ccfDNA Tubes?
FAQ ID - 3642
Is double centrifugation required for plasma processing?
FAQ ID - 3643
How long and at which temperature can plasma generated from whole blood in PAXgene Blood ccfDNA Tubes be stored?
FAQ ID - 3644
What should I do if cryoprecipitates form while thawing a frozen plasma sample?
FAQ ID - 3646
Which plasma volumes can I process with the QIAsymphony PAXgene Blood ccfDNA protocols?
FAQ ID - 3648
How many extractions can I perform with a QIAsymphony PAXgene Blood ccfDNA Kit, using the 2.4 ml or the 4.8 ml protocols?
FAQ ID - 3649
How long after first use can a reagent cartridge be stored for further use?
FAQ ID - 3650
For what type of sample material is the QIAsymphony PAXgene Blood ccfDNA Kit intended?
FAQ ID - 3652
Which tubes are recommended for the QIAsymphony sample rack
FAQ ID - 3653
Which protocol line should I use to process plasma samples?
FAQ ID - 3654
Does the QIAsymphony PAXgene Blood ccfDNA Kit also allow eluting ccfDNA in less than 60 µl?
FAQ ID - 3656
How should eluates produced with the PAXgene Blood ccfDNA System be stored?
FAQ ID - 3657
Which downstream applications have been tested with ccfDNA purified from whole blood collected and processed with the PAXgene Blood ccfDNA System?
FAQ ID - 3658
What is the shelf life of the QIAsymphony PAXgene Blood ccfDNA Kit?
FAQ ID - 3651
Which elution volumes are available with the QIAsymphony PAXgene Blood ccfDNA Kit?
FAQ ID - 3655
Why is a blood collection set required to collect blood into the PAXgene Blood ccfDNA Tube?
FAQ ID - 3628
Can reagent from the PAXgene Blood ccfDNA Tube flow back into the patient’s arm?
FAQ ID - 3629
Can I use a different brand blood collection set with the PAXgene Blood ccfDNA Tube?
FAQ ID - 3630
What is the blood draw volume of the PAXgene Blood ccfDNA Tube
FAQ ID - 3631
What is the difference between RT² Profiler PCR Array and RT² Profiler PCR Array PLUS version?
FAQ ID - 3685
Where can I find the gene list for a specific RT² Profiler PCR Array?
FAQ ID - 3684
Is it possible to store nucleic acids extracted using the SpeedXtract Virus Kit?
FAQ ID - 3680
Total RNA from what sample types are compatible with the QIAseq miRNA Library Kit?
FAQ ID - 3660
Can miRNA sequencing libraries be prepared from total RNA isolated from heparinized plasma samples?
FAQ ID - 3661
Are any components of the QIAseq miRNA Library Kit interchangeable with components from other QIAGEN kits?
FAQ ID - 3662
Can I still perform sequencing if my miRNA sequencing library concentrations are too low to obtain a 4 nM library?
FAQ ID - 3666
What method is used to eliminate adapter dimers from the QIAseq miRNA library?
FAQ ID - 3667
Does a QIAseq miRNA library include hY4 Y RNA?
FAQ ID - 3670
What is the sequence of the QIAseq miRNA NGS 3’ Adapter?
FAQ ID - 3671
Do the covered regions in the BED file include the primers?
FAQ ID - 3689
What do the GTIN, UDI and REF abbreviations mean on the label of any QIAGEN box?
FAQ ID - 3697
Do eluates generated with the PAXgene Blood miRNA Kit contain residual genomic DNA?
FAQ ID - 3637
Do you have a protocol for high-throughput purification of plasmid DNA using the BioRobot Universal System?
FAQ ID -1191
Is the surface tension of the crystallization supports of the EasyXtal 15-Well Tools similar to that of a siliconized cover slide?
FAQ ID -1107
Is the pH indicated in the formulation sheet for Screening Suite buffers the final pH?
FAQ ID -1123
What are the applications the EasyXtal 15-Well Tool can be used for?
FAQ ID -1111
Is the EasyXtal Tool compatible with liquid-handling systems and visualization robots?
FAQ ID -1118
Is it possible to collect intact mitochondria before or after the density gradient centrifugation step of the Qproteome Mitochondria Isolation protocol?
FAQ ID -1187
What cell lines have been tested with the Fastlane Cell cDNA Kit?
FAQ ID -1175
What does 'QIAGEN Certified Solution' for Protein Crystallization mean?
FAQ ID -1121
How many protein crystallization conditions are available at QIAGEN?
FAQ ID -1119
How much detection reagent do you generally recommend for LiquiChip Assays?
FAQ ID -1159
How many cells can be used with the Qproteome Mitochondria Isolation Kit, and how much mitochondrial protein can be expected?
FAQ ID -1189
How many drops can be set up on a protein crystallization support?
FAQ ID -1110
How many LiquiChip beads are recommended for an xMAP experiment?
FAQ ID -1161
How much solution can I add to an EasyXtal 15-Well Tool reservoir well?
FAQ ID -1114
In which formats are crystallization condition Screening Suites available?
FAQ ID -1127
If I cannot find a QuantiTect Primer Assay for my target gene in rat, can I use the assays for the orthologous genes in mouse and human?
FAQ ID -1145
If a QuantiTect Primer Assay has more than one version number, does this mean that earlier assay versions may not work?
FAQ ID -1136
How sensitive is the LiquiChip Reader?
FAQ ID -1157
How much time is needed for a LiquiChip assay point measurement?
FAQ ID -1162
Why are there various different QuantiTect Primer Assays for my gene (previous versions, transcript variants)?
FAQ ID -1133
What is the delivery time for QuantiTect Primer Assays?
FAQ ID -1142
Where can I find the composition tables for solutions used in individual Crystallization Screening Suites?
FAQ ID -1124
What kind of strategy for initial screening of protein crystallization conditions do you recommend?
FAQ ID -1120
When searching for a QuantiTect Primer Assay for my gene of interest, why do I only find the assay for human but not for rat?
FAQ ID -1139
What type of magnet do you recommend for handling the magnetic beads in the Qproteome Plasma Membrane Protein protocol?
FAQ ID -1193
What starting material can be used with the Qproteome Mitochondria Isolation Kit?
FAQ ID -1184
Can the EZ1 DNA Investigator Tip Dance protocol cope with any solid matrix remaining in the sample tube?
FAQ ID -1153
Are EasyXtal 15-Well Tools stackable?
FAQ ID -1116
Why can I not find the QuantiTect Primer Assay in GeneGlobe that I had previously ordered from QIAGEN?
FAQ ID -1138
Which QuantiTect Primer Assay should I choose for my gene of interest?
FAQ ID -1135
Why does the QuantiTect Primer Assay for my gene of interest have only one version number?
FAQ ID -1134
Which chemical products do you use to prepare screening solutions for protein crystallization?
FAQ ID -1122
Will proteins eluted in the Qproteome Plasma Membrane Protein protocol be modified, e.g. with the ligand?
FAQ ID -1195
Will the ligand in the Qproteome Plasma Membrane Protein protocol be specific for plasma membrane exclusively?
FAQ ID -1198
Will integral, peripheral as well as lipid-anchored plasma membrane proteins be isolated with the Qproteome Plasma Membrane Protein Kit?
FAQ ID -1199
Why is there no QuantiTect Primer Assay for my gene of interest?
FAQ ID -1140
What is the EasyXtal 15-Well Tool made of?
FAQ ID -1106
What is the dynamic range of detection of the LiquiChip Reader?
FAQ ID -1158
Can the nuclei and cell debris fraction generated in the QProteome Mitochondria isolation procedure be used for further fractionation?
FAQ ID -1185
Can the EZ1 DNA Forensic Card be used with the EZ1 DNA Investigator Kit?
FAQ ID -1151
Can the EasyXtal 15-Well Tools be reused?
FAQ ID -1117
Can the Qproteome Plasma Membrane Protein Kit be used with suspension cells?
FAQ ID -1194
Can your Tag antibody recognize rat MAPK1 also?
-100
Can xMAP kits from other Luminex partners be used on the LiquiChip System purchased from QIAGEN?
FAQ ID -1156
Can the remaining fractions obtained in the Qproteome Plasma Membrane Protein procedure be used in downstream assays?
FAQ ID -1197
Can the Spin Columns of the Qproteome Albumin/IgG Depletion Kit be reused?
FAQ ID -1173
Are scoring sheets available for each crystallization condition Screening Suite?
FAQ ID -1125
Can mitochondria separated with the Qproteome Mitochondria Isolation Kit be used for downstream DNA isolation?
FAQ ID -1188
Are protein modifications such as glycosylation problematic when using the Qproteome Plasma Membrane Protein Kit?
FAQ ID -1196
Can acetone be used for precipitation of protein from Buffer RLT lysates generated with RNeasy Kits?
FAQ ID -1164
Can I increase the annealing temperature recommended for QuantiTect Primer Assays used with the QuantiTect SYBR Green PCR Kits?
FAQ ID -1146
How are NeXtal deep-well blocks sealed? Can they be resealed?
FAQ ID -1128
Does the EasyXtal Tool conform to an SBS format?
FAQ ID -1115
Do you have WGA protocols for starting materials not mentioned in the REPLI-g Handbooks?
FAQ ID -1160
How easy is it to scoop up a protein crystal with the lip around the crystallization supports of the EasyXtal 15-Well Tools?
FAQ ID -1113
Do you have a protocol for the purification of 6xHis-tagged proteins using BioSprint?
FAQ ID -1167
How did you determine that your AllStar Negative Control siRNA binds to RISC?
FAQ ID -1180
How is the quality of the crystallization condition Screening Suites guaranteed?
FAQ ID -1126
How do I use EasyXtal Crystallization Tools?
FAQ ID -1105
How does the sealing efficiency of the crystallization supports of the EasyXtal 15-Well Tool compare to grease?
FAQ ID -1109
Do you have a kit for protein isolation from mitochondria prepared with your Qproteome Mitochondria Isolation Kit?
FAQ ID -1190
Do you have a kit for the isolation of RNA from formalin-fixed, paraffin-embedded samples?
FAQ ID -1174
Do I need an ultracentrifuge for the density gradient centrifugation step in the Qproteome Mitochondria Isolation protocol?
FAQ ID -1186
Do protein crystals stick to the walls of cavities when using the DropGuard crystallization supports?
FAQ ID -1108
Do you have a protocol for evaluating pipetting accuracy of the BioRobot EZ1?
FAQ ID -1131
Do you have a protocol for purification of DNA from stool samples for pathogen detection using BioSprint Instruments?
FAQ ID -1165
Do you have a protocol for purification of DNA from stool samples using BioSprint Instruments?
FAQ ID -1166
Do you have a protocol for evaluating temperature accuracy of the BioRobot EZ1?
FAQ ID -1168
Which competent E. coli cells should I use to amplify the SureSilencing shRNA Plasmids?
FAQ ID -2890
Which analyses can be performed with the PyroMark Q96 ID software version 1.0?
FAQ ID -2845
Does the same U1 promoter in the SureSilencing shRNA Plasmids express shRNA in human, mouse, and rat cell lines?
FAQ ID -2887
How can I use the SureSilencing shRNA Plasmids for stable transfection and knock down in a cell line that is already resistant to both neomycin and puromycin?
FAQ ID -2895
Can I use my own siRNA design?
FAQ ID -2897
Can I order the nucleotides from PyroMark Gold Reagents separately?
FAQ ID -2827
Can I reclone the insert sequences from the SureSilencing shRNA Plasmids into a viral-based vector?
FAQ ID -2896
Can I directly transfect SureSilencing shRNA Plasmids into my cells?
FAQ ID -2899
Do any of the SureSilencing shRNA Plasmids contain inducible promoters?
FAQ ID -2888
Can PyroMark Gold reagents be vortexed?
FAQ ID -2844
What are the features of the new PyroMark Q96 ID software 2.5?
FAQ ID -2867
What cell line does QIAGEN use for quality control testing of the SureSilencing shRNA Plasmids?
FAQ ID -2885
Should I use the SureSilencing shRNA Plasmids with the neomycin-resistance marker, the puromycin-resistance marker, or the GFP reporter gene?
FAQ ID -2882
What else do I need to complete an experiment using the SureSilencing shRNA Plasmids?
FAQ ID -2889
What cell lines can I use with the SureSilencing shRNA Plasmids?
FAQ ID -2891
How long should I wait after transfection before enriching my transfected cells, validating knock down, and looking for phenotypic changes?
FAQ ID -2893
Is the expression level of miRNA precursors lower compared to that of mature miRNAs?
FAQ ID -2807
How many times can the CDTs, NDTs, and RDTs be used?
FAQ ID -2865
What is the PyroMark Q96 Data Converter?
FAQ ID -2868
What is the pyrosequencing data exchange tool for?
FAQ ID -2864
What is the composition of Buffer APP?
FAQ ID -2804
What is the composition of Buffer FRN?
FAQ ID -2802
What is the composition of Buffer APL?
FAQ ID -2803
Which analyses can be performed with the PyroMark Q96 MD software?
FAQ ID -2866
Which PyroMark Gold Q96 Reagent should be used for which instrument and application?
FAQ ID -2836
What is the SureSilencing shRNA Plasmids guarantee?
FAQ ID -2898
What primers may I use to sequence the shRNA cassette within the SureSilencing shRNA Plasmids?
FAQ ID -2886
Does the PyroMark CpG LINE assay target mouse transposons as well?
-1
How do I find the SureSilencing shRNA Plasmids for my gene of interest?
FAQ ID -2883
How are the SureSilencing shRNA Plasmids shipped?
FAQ ID -2884
What is the concentration of SureENTRY Transduction Reagent?
FAQ ID - 3708
Is it possible to get a Custom RT2 Profiler PCR Array, cat. # 330131, with primer assays targeting transcripts of different species, e.g. assays targeting transcripts from mouse and assays targeting transcripts from human, on one plate?
FAQ ID - 3710
Is it possible to purchase Buffer VXL which is part of the QIAamp cador Pathogen Mini Kit, (Cat No.: 54104, 54106) separately?
FAQ ID - 3712
What sample tubes can I use on my QIAsymphony SP system?
FAQ ID - 3713
How can I decontaminate my QIAsymphony SP / AS system?
FAQ ID - 3714
Can other QIAGEN buffers from non-UCP kits (e.g. AW1 instead of AUW1) be used with the QIAamp UCP DNA Micro Kit (cat. no. 56204)?
FAQ ID - 3717
How is the Pyromark Q48 cartridge to be cleaned?
FAQ ID - 3718
Can the disc and absorber strips for the Pyromark Q48 be reused?
FAQ ID - 3720
What is the dead volume of the cartridges in PyroMark Q48?
FAQ ID - 3719
How much DNA is needed in the PyroMark Q48?
FAQ ID - 3721
Can I use PyroMark Q48 for diagnostics?
FAQ ID - 3722
Can I transfer my assays from PyroMark Q24/Q96 to PyroMark Q48 Autoprep?
FAQ ID - 3723
Can I analyze my Next Generation Sequencing (NGS) samples on the QIAxcel for Quality control?
FAQ ID - 3724
Is an additional proteinase K digest possible with DNeasy PowerSoil Kit and DNeasy PowerLyzer Kit?
FAQ ID - 3725
Can I buy the 1 ml Bead Plates from the DNeasy UltraClean 96 Microbial Kit (384) separately?
FAQ ID - 3726
Does QIAGEN offer an anti-GFP gapmer control?

FAQ ID - 3739

 


Why do I observe a CT shift for the Internal Control in some samples in comparison to the CT value for the Internal Control of the dilution series of the Male Control DNA M1?
FAQ ID - 3745
How long can I store the dilutions of the Male Control DNA M1 standards at 2–8°C?
FAQ ID - 3744
Do I need to buy special kits for the QIAcube Connect?
FAQ ID - 141485
Can I program my own protocols for the QIAcube Connect?
FAQ ID - 141486
Can the QIAcube Connect rotor and/or buckets be removed for cleaning?
FAQ ID - 141491
Can purification columns from other suppliers be processed on the QIAcube Connect?
FAQ ID - 141487
How can I get software updates for the QIAcube Connect?
FAQ ID - 141494
Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube Connect?
FAQ ID - 141496
Do I need to discard partially used QIAcube Connect tip racks?
FAQ ID - 141499
How can I decontaminate the QIAcube Connect system?
FAQ ID - 141500
Can the QIAcube Connect heater/shaker be used independently from protocol runs?
FAQ ID - 141502
How can I load new protocols onto the QIAcube Connect?
FAQ ID - 141501
What dedicated QIAcube Connect kits are available?
FAQ ID - 141504
Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube Connect?
FAQ ID - 1414507
Where can I find the ordering information for QIAcube Connect accessories such as shaker rack plugs, rack labeling strips, reagent bottle racks, 30 ml reagent bottles, rotor-adapter holder, rotor adapters, sample tubes CB, sample tubes RB and spin-column adapter rings?
FAQ ID - 141505
Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube Connect?
FAQ ID - 141506
Which QIAcube Connect standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet?
FAQ ID - 141508
How often should the o-ring for the pipettor tip adapter be changed on the QIAcube Connect?
FAQ ID - 141509
Can the CB and RB sample tubes be used interchangeably?
FAQ ID - 141510
Which network settings are supported by the QIAcube Connect and Q-Base?
FAQ ID - 141517
Why can I not find the Q-Base device in the device list during the first installation?
FAQ ID - 141516
Why can’t I see my QIAcube Connect instrument in the QIAcube Connect App?
FAQ ID - 1414518
Why is the QIAcube Connect App reacting so slowly?
FAQ ID - 141519
Why are not all applications/kits/protocol files visible in the run setup (QIAcube Connect App)?
FAQ ID - 1414520
Why does the connection test fail?
FAQ ID - 141522
How can I accurately determine the amount of input material for the EpiTect Hi-C Kit?
FAQ ID - 143065
What sample source do you support?
FAQ ID - 143066
How long does it take to complete the EpiTect Hi-C protocol?
FAQ ID - 143068
How many reactions are in a kit?
FAQ ID - 143067
Can I use cells that have been previously crosslinked and frozen (e.g., for a ChIP or ChIP-seq experiment) as input material for the EpiTect Hi-C protocol?
FAQ ID - 143069
Can I substitute the endonuclease used in the Hi-C Digestion step with another endonuclease?
FAQ ID - 143070
Are there stopping points in the Hi-C workflow?
FAQ ID - 143071
With which platform can I perform my sequencing?
FAQ ID - 143072
With which read length should the EpiTect Hi-C NGS libraries be sequenced
FAQ ID - 143073
How do I know if my Hi-C sample is a good candidate for deep sequencing?
FAQ ID - 143074
What kind of analysis does the EpiTect Hi-C Data Analysis Portal perform?
FAQ ID - 143076
Can I use my own pipeline to analyze results from my EpiTect Hi-C experiments?
FAQ ID - 143078
How critical is the time of the temperature change from 60°C to 80°C?
FAQ ID - 143768
Is it possible to use Lyse&Spin baskets?
FAQ ID - 143762
Why is DTT used only for semen samples?
FAQ ID - 143761
Can the Repli-g Advanced DNA Single Cell Kit also be used for bacterial cells?
FAQ ID - 143079
Can the lysis volume be reduced for higher sensitivity?
FAQ ID - 143763
What if only one heater shaker is available?
FAQ ID - 143764
Why is there no male DNA obtained from sexual assault samples?
FAQ ID - 143767
If 400 µl are used as lysis volume, how do I concentrate the lysate? (Otherwise the DNA would be too diluted.)
FAQ ID - 143766
Why is the QIAamp Viral RNA Mini Kit (50) currently unavailable?
FAQ ID -147395
What RNAs has QIAseq FastSelect been designed to remove?
FAQ ID -147910
How does QIAseq FastSelect work?
FAQ ID -147907
Is QIAseq FastSelect truly as easy as combining a reagent with RNA and ramping down the temperature?
FAQ ID -147909
Will QIAseq FastSelect work in other species?
FAQ ID -147911
What library prep kits has QIAseq FastSelect been tested with?
FAQ ID -147912
I am using an RNA library prep kit that is not listed in the QIAseq FastSelect handbook. Will QIAseq FastSelect work with my kit?
FAQ ID -147913
What RNA range has the QIAseq FastSelect been tested with?
FAQ ID -147914
Can QIAseq FastSelect Plant or QIAseq FastSelect Yeast be combined with FastSelect 5S/16S/23S?
FAQ ID -147915
Is it possible to test the efficiency of FastSelect rRNA removal by using a Bioanalyzer, etc.?
FAQ ID -147916
Is plasmid DNA purified with QIAGEN Plasmid Purification Kits suitable for in vitro transcription?
FAQ ID -1
How should I quantify RNA isolated with RNeasy Kits?
FAQ ID -32
When is chloramphenicol amplification of plasmids performed?
FAQ ID -3
What are the expected DNA yields from different tissues using the QIAamp DNA Mini Kit?
FAQ ID -45
What are the most commonly used protease inhibitors?
FAQ ID -53
How should I propagate pQE expression plasmids?
FAQ ID -58
How can I increase the amount of soluble recombinant protein in E. coli expression?
FAQ ID -64
How can I remove imidazole from a protein sample?
FAQ ID -91
Why does my isolated RNA have a low OD 260/280 ratio?
FAQ ID -97
How can I avoid little or no RNA yields when using an RNeasy Kit?
FAQ ID -28
Do I need to use an RNase inhibitor in my RT reaction?
FAQ ID -119
Can I use QIAprep Miniprep kits for low-copy plasmids and cosmids?
FAQ ID -127
Can I use the QIAquick PCR Purification Kit for restriction enzyme cleanup?
FAQ ID -130
How does imidazole affect my quantitation of protein?
FAQ ID -132
Can a QIAquick Gel Extraction Kit be used to obtain RNA from a formaldehyde gel?
FAQ ID -133
What is the difference between disruption and homogenization in the RNeasy System?
FAQ ID -139
What is the size of genomic DNA that is obtained with QIAGEN Genomic-tips?
FAQ ID -142
Should I use Ni-NTA Agarose in column or batch format for purification of 6xHis-tagged proteins?
FAQ ID -147
What is the genotype of the EZ Competent Cells?
FAQ ID -157
What are the restriction sites of the pDrive Vector in the QIAGEN PCR Cloning Kit?
FAQ ID -160
What are the molecular weights of proteins in the 6xHis Protein Ladder?
FAQ ID -169
How can I optimize the transfection of oligos and large plasmids using PolyFect Transfection Reagent?
FAQ ID -176
How can I improve recoveries when using the QIAquick Kits?
FAQ ID -180
How can I improve transfection efficiency using Effectene Transfection Reagent?
FAQ ID -181
What is the principle behind Effectene Transfection Reagent?
FAQ ID -184
What is the composition of Buffer EB?
FAQ ID -199
Is it possible to modify the transduction protocol for Cignal Lenti Reporter Assays to save some pipetting steps?
FAQ ID - 3709
Can I store the QIAseq Beads of the QIAseq Targeted DNA Panel at -20°C?
FAQ ID - 3711
What is the difference between the QIAamp UCP DNA Micro Kit (cat. no. 56204) and RNeasy UCP Kits (cat. no. 73934) and non-UCP QIAGEN purification kits?
FAQ ID - 3715
Can I use carrier RNA with the RNeasy UCP Micro Kit (cat. no. 73934)?
FAQ ID - 3716
How can I check for purity of RNA isolated using RNeasy Kits?
FAQ ID -1023
What is the recipe for 1x PBS solution?
FAQ ID -1030
Where can I find a protocol for cleanup of already purified plasmid DNA?
FAQ ID -1031
How do I perform an Acetone Precipitation for concentrating and desalting protein samples?
FAQ ID -1035
What is the difference between Buffers RLT and RLT Plus?
FAQ ID -1043
What is the largest PCR amplicon that can be amplified with the HotStar HiFidelity Polymerase Kit?
FAQ ID -1047
Are the CoralLoad dyes in the HotStarTaq Plus PCR Buffer visible when loading small amounts of PCR product onto a gel?
FAQ ID -1051
Do I have to use CoralLoad Gel loading dye when using your HotStarTaq Plus DNA Polymerase?
FAQ ID -1052
How can I increase DNA concentration using QIAprecipitators of the HiSpeed Plasmid Kits?
FAQ ID -1061
What should I use as a standard for absolute quantification in real-time PCR?
FAQ ID -1085
What does transcript variant mean when searching for specific QuantiTect Primer Assays?
FAQ ID -1137
What is a QuantiTect Primer Assay?
FAQ ID -1141
Do QuantiTect Primer Assays contain SYBR Green dye?
FAQ ID -1143
What is the smallest and the largest protein that you tested for expression with the EasyXpress Insect Kit II?
FAQ ID -1218
What are the new features of the EasyXpress Insect Kit II compared to the original Protein Synthesis Insect kit?
FAQ ID -1220
What are your recommendations for PCR template preparation for use with the EasyXpress Insect Kit II?
FAQ ID -1221
Do you have a protocol for transfection of suspension cell lines (Jurkat and K562) with siRNA?
FAQ ID -1250
Do you have a protocol for transfection of macrophage cell lines (J774.A1 and RAW 264.7) with siRNA?
FAQ ID -1251
What is the pH value of QIAGEN's Nuclease-Free Water?
FAQ ID -1290
Why is there DNA in the no-template control reaction when using the standard REPLI-g procedure, but not when using the UltraFast procedure?
FAQ ID -1327
Will the REPLI-g UltraFast Mini Kit reaction always yield 10 µg DNA?
FAQ ID -1328
Can the bacterial pellet in the QuickLyse Miniprep procedure be resuspended by pipetting up and down or shortening the vortexing time?
FAQ ID -1352
How can I increase the DNA yield from low-copy plasmids using the QuickLyse Miniprep Kit?
FAQ ID -1354
What is the composition of elution buffer QLE in the QuickLyse Miniprep Kit?
FAQ ID -1356
How do I resuspend my FlexiPlate siRNAs?
FAQ ID -1360
Are there any specific cleaning solutions recommended for the QIAcube?
FAQ ID -1416
Why is a 2-step (and not a 3-step) cycling protocol recommended for QuantiFast SYBR Green PCR Kits?
FAQ ID -1450
Can tissue homogenized in Buffer RLT Plus instead of Buffer RLT be used with the AllPrep DNA/RNA/Protein Mini Kit?
FAQ ID -1577
What can be done if the protein pellet does not solubilize completely using the AllPrep DNA/RNA/Protein Mini Kit?
FAQ ID -1580
What is the binding capacity of the Ni-NTA Superflow Cartridges?
FAQ ID -1603
How comparable is CoralLoad gel loading dye contained in various QIAGEN PCR Kits to Sigma Red?
FAQ ID -1644
Do I need to resuspend FlexiTube siRNAs in siRNA Resuspension buffer?
FAQ ID -1659
Are FlexiTube siRNA and FlexiTube GeneSolution also available for rat siRNAs?
FAQ ID -1664
What is the composition of Buffer PE?
FAQ ID -1691
What is the upper length of PCR products that can be amplified with TopTaq DNA Polymerase and Master Mix Kits?
FAQ ID -1738
What is the minimum sample volume required for the QIAxcel System and QIAxcel Advanced?
FAQ ID -1852
What are the dimensions and weight of the QIAsymphony SP System?
FAQ ID -1901
Are there any specific cleaning solutions recommended for the QIAsymphony SP?
FAQ ID -1913
How much sample can be used with the RNeasy Plus 96 Kit?
FAQ ID -1992
What is the composition of Buffer P2?
FAQ ID -203
A white precipitate has formed in my 10x RT buffer. Is it still ok to use?
FAQ ID -216
For how long are BioMag Particles stable?
FAQ ID -262
I used to buy BioMag particles from a company by another name? Are your BioMag particles the same thing?
FAQ ID -263
Can I clean up my DNase treated RNA samples using RNeasy columns?
FAQ ID -286
How can one determine the optimal annealing temperature for a specific PCR assay?
FAQ ID -288
What is the composition of the QIAGEN Multiplex PCR Buffer?
FAQ ID -289
Can I use HEPES buffer instead of phosphate in my Ni-NTA column?
FAQ ID -291
Can I use a centrifuge instead of vacuum when using the QIAquick 96 PCR Purification Kit?
FAQ ID -293
What are the ideal and the largest PCR amplicon sizes when using the EpiTect MSP Kit?
FAQ ID -2004
What is elution Buffer ATE of the QIAsymphony DNA Investigator Kit?
FAQ ID -2029
How can a C-terminal 6xHis-tagged QIAgene be expressed?
FAQ ID -2041
Why is there more than one QIAgene for my gene of interest?
FAQ ID -2044
Is a passive internal reference dye, like ROX, required on the Rotor-Gene Q to obtain reproducible results?
FAQ ID -2079
Does the Rotor-Gene Q software have the capacity to export data?
FAQ ID -2089
Why is a 2-step (and not a 3-step) cycling protocol recommended for Rotor-Gene SYBR Green Kits?
FAQ ID -2122
Does carrier RNA have to be added using the QIAsymphony Virus Blood 200 Protocol?
FAQ ID -2163
Can I use the RNeasy Microarray Tissue Mini Kit for all tissue types?
FAQ ID -2190
How is the RNeasy Microarray Tissue Mini Kit different from the classic RNeasy Mini Kit?
FAQ ID -2192
Why is EvaGreen instead of SYBR Green used as fluorescent dye in the Type-it HRM PCR Kit?
FAQ ID -2196
Why do I need normalization using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2198
What is the difference between probability and typicality in the Rotor-Gene ScreenClust HRM Software?
FAQ ID -2203
What buffer conditions give the best resolution for agarose gel electrophoresis?
FAQ ID -2256
How much DNA should be loaded per well of an agarose gel?
FAQ ID -2259
Why do I have wavy DNA bands on my agarose gel?
FAQ ID -2260
What is the "seed sequence" of a miRNA?
FAQ ID -2262
Are you able to supply a Certificate of Analysis for the KRAS and EGFR PCR kits?
FAQ ID -2301
Which Rotor-Gene Q rotor should be used with the EGFR RGQ PCR kit?
FAQ ID -2315
How is the RNeasy Plus Universal Kit different from the RNeasy Plus Kit?
FAQ ID -2340
How does the gDNA eliminator solution of the RNeasy Plus Universal Kit work?
FAQ ID -2341
How long can I store my purified DNA/RNA isolated with the AllPrep DNA/RNA FFPE kit?
FAQ ID -2350
Do you have any information or guidelines regarding the choice of reference genes for real-time PCR?
FAQ ID -2371
Can I buy QIAquick and MinElute columns separately?
FAQ ID -2460
What is the PAXgene 96 filter plate?
FAQ ID -2486
Is RNA from red blood cells also isolated when RNA is extracted from a PAXgene Bone Marrow RNA Tube?
FAQ ID -2509
Is the 260/230 nm absorbance ratio specified for RNA derived from the PAXgene Bone Marrow RNA System?
FAQ ID -2514
How long is the fixation time?
FAQ ID -2519
What is the nature of the heterologous Internal Amplification Control and Positive Control in the mericon Assay kits?
FAQ ID -2543
Are there any accessories required for sample disruption with the TissueLyser LT?
FAQ ID -2551
Why do the Internal Control templates for extraction (Internal Control DNA or RNA [High conc.]) have a 10x higher concentration than the IC templates provided with the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2603
During data analysis, how should the threshold be set in the Yellow channel on the Rotor-Gene Q cycler to analyze the Internal Control from the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit
FAQ ID -2607
What is the SBTengine® software?
FAQ ID -2628
How do I create a workspace that is free of DNA contamination, prior to carrying out a qPCR experiment?
FAQ ID -2654
What is the recommended solution in which to store RNA samples that will be used as templates for cDNA synthesis?
FAQ ID -2659
How can I avoid or remove genomic DNA contamination from the total RNA preparation?
FAQ ID -2662
What are the differences between one-step and two-step RT--PCR?
FAQ ID -2666
What is qPCR?
FAQ ID -2668
What are the common primer and probe chemistries utilized for qPCR assays?
FAQ ID -2669
What is the purpose of the ROX and fluorescein dyes, also known as passive reference dyes, in qPCR master mixes?
FAQ ID -2671
What negative controls are typically included in qPCR and/or qRT-PCR experiments?
FAQ ID -2672
What positive controls are typically included in qPCR and/or qRT-PCR experiments?
FAQ ID -2673
What are the guidelines for choosing a housekeeping gene for normalizing qPCR results?
FAQ ID -2674
Why is 18S ribosomal RNA (rRNA) used as a housekeeping gene to normalize sample-to-sample, systematic variation in qPCR assays?
FAQ ID -2675
How does HotStart PCR help minimize nonspecific amplification events?
FAQ ID -2676
What is a dissociation curve, and why is it important to run a dissociation curve, following qPCR using SYBR Green chemistry?
FAQ ID -2678
What is the delta Rn value?
FAQ ID -2681
What is the threshold cycle or Ct value?
FAQ ID -2682
Why are my qPCR Ct values too low (< 12) in my qRT-PCR Assay?
FAQ ID -2684
Why are my qPCR Ct values too high (> 35 or not detectable) in my qRT-PCR assay?
FAQ ID -2685
Why is my no template control (NTC) real-time Ct value < 35 cycles in my qPCR Assay?
FAQ ID -2686
Why do my qPCR amplification curves or plots decrease in fluorescence intensity after the saturation phase?
FAQ ID -2689
Why do I see multiple high-intensity peaks in my qPCR dissociation curve at temperatures less than 70 ºC?
FAQ ID -2690
What is the standard curve method for qPCR assay data analysis? How is the standard curve method for qPCR assay data analysis performed?
FAQ ID -2691
What is the difference between Absolute Quantification and Relative Quantification in qPCR, using the standard curve approach?
FAQ ID -2692
What is the comparative or ??Ct method for qPCR assay data analysis? How is the comparative or ??Ct method for qPCR assay data analysis performed?
FAQ ID -2693
How do I determine the amplification efficiency of my qPCR assay?
FAQ ID -2694
How do I determine the linear dynamic range of my qPCR or qRT-PCR assay?
FAQ ID -2696
What is the best approach for determining where to set the CT threshold when you have >15 samples? Is it best to go through all of them, looking for a range of best fit, and then just choose one value that fits all of them?
FAQ ID -2705
What do I need to complete a RT² qPCR Primer Assay?
FAQ ID -2707
How can I prevent the evaporation of reaction volume from the wells in the miScript miRNA PCR array?
FAQ ID -2729
What is the minimum amount of genomic DNA required for analysis using EpiTect Methyl qPCR Arrays?
FAQ ID -2739
How do you design your EpiTect ChIP qPCR Assay primers? Do they work with SB GR?
FAQ ID -2749
How can I identify the binding sites of my transcription factor using ChIP?
FAQ ID -2753
At what point can I stop and freeze my samples when using ChIP?
FAQ ID -2754
What is RNA interference (RNAi)?
FAQ ID -2755
What are the key factors for success in an RNAi experiment?
FAQ ID -2758
What criteria should one use in choosing between siRNA versus shRNA for their studies?
FAQ ID -2771
How does stable transfection of a shRNA-encoding plasmid work?
FAQ ID -2779
What are the SureSilencing shRNA Plasmids?
FAQ ID -2784
What is the composition of Buffer S3?
FAQ ID -2788
What is the composition of Buffer ETR?
FAQ ID -2789
What is the composition of Buffer BB?
FAQ ID -2790
What is the composition of Buffer RLT plus?
FAQ ID -2794
What is the composition of Buffer RLC?
FAQ ID -2795
What is the composition of Buffer RPE?
FAQ ID -2797
What is the composition of Buffer RWT?
FAQ ID -2798
What is the composition of gDNA Eliminator Solution?
FAQ ID -2799
What is the composition of Buffer RDD?
FAQ ID -2800
What is the composition of Buffer PKD?
FAQ ID -2801
What is the composition of Buffer ALO?
FAQ ID -2805
What is the composition of RBC Lysis Solution?
FAQ ID -2808
What is the composition of Cell Lysis Solution?
FAQ ID -2809
What is the composition of Protein Precipitation Solution?
FAQ ID -2810
What is the composition of Cell Suspension Solution?
FAQ ID -2811
What is the composition and concentration of Glycogen Solution in Gentra Puregene Kits?
FAQ ID -2812
What is the composition of DNA Hydration Solution in Gentra Puregene Kits?
FAQ ID -2813
What is the meaning of the abbreviations NDT and CDT and what are the differences?
FAQ ID -2841
What are the changes of PyroMark Assay Design Software Version 2.0 compared to 1.0?
FAQ ID -2849
Can I use fluorescence microscopy to assess the transfection efficiency of the SureSilencing shRNA Plasmids with GFP in my model cell line of interest?
FAQ ID -2894
Do tissue samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction?
FAQ ID -2926
What is the composition of Buffer G2?
FAQ ID -2943
What is the composition of Buffer B1?
FAQ ID -2944
What is the composition of Buffer B2?
FAQ ID -2945
How much DNA does a human cell contain?
FAQ ID -2948
How much RNA does a bacterial cell contain?
FAQ ID -2949
How much DNA and RNA can be expected from human blood cells?
FAQ ID -2950
What is the cellular composition of human blood?
FAQ ID -2951
How can I desalt and concentrate a protein fraction by trichloroacetic acid (TCA) precipitation?
FAQ ID -2954
Is there a risk of crosstalk from the FAM channel to the VIC channel if the residual DNA target is present in very high copy numbers?
FAQ ID -2980
Why is the final heat denaturation step so important with the QIAsymphony PAXgene Blood RNA protocol?
FAQ ID -2987
What is the sequence of the miScript Universal Primer?
FAQ ID -2994
How can I keep my centrifuge tubes endotoxin-free?
FAQ ID -301
Can Buffers N3 and P3 be used interchangeably?
FAQ ID -310
Are QIAprep and QIAquick Spin columns interchangeable?
FAQ ID -311
Can I store agarose gel slices containing DNA for gel extraction at a later point?
FAQ ID -313
How can QIAGEN Protease and Proteinase K be inactivated?
FAQ ID -315
Does the 5x OneStep RT-PCR Buffer contain BSA?
FAQ ID -326
What is the origin of replication and the plasmid copy number of the pQE vectors?
FAQ ID -338
Is QIAGEN carrier RNA sold separately?
FAQ ID -351
What is the white insoluble precipitate in my resuspended plasmid DNA pellet?
FAQ ID -352
What is the composition of PBS?
FAQ ID -361
How can I express toxic protein in E. coli?
FAQ ID -373
How can I improve DNA yields from very tough tissues using the DNeasy Blood & Tissue Kit or the QIAamp DNA Mini Kit?
FAQ ID -374
What are the dissociation constants for the Anti-His Antibodies?
FAQ ID -385
What is the average molecular weight of a siRNA, and how do I convert uM to ug values?
FAQ ID -388
Can siRNA silence bacterial genes?
FAQ ID -395
How should I reconstitute the lyophilized RNase-Free DNase Set with the RNeasy 96 kit?
FAQ ID -3003
Is it possible to use a standard processor — the kind used routinely for formalin-fixed samples — for dehydration and paraffin infiltration of PAXgene Tissue treated samples?
FAQ ID -3032
Can I use Tungsten Carbide beads for RNA extraction protocols using QIAGEN Kits?
FAQ ID -3053
Where can I find the ordering information for QIAcube accessories such as Shaker Rack plugs, Rack labeling strips, Reagent bottle rack, 30mL Reagent bottles, Rotor Adapter holder, Rotor adapters, Sample tubes CB, Sample tubes RB and Spin column adapter rings?
FAQ ID -3054
What is the part number for O-rings for QIAsymphony SP and AS instruments? How often should the O-rings be replaced?
FAQ ID -3055
What is the thermal output for a BioRobot MDx and BioRobot Universal?
FAQ ID -3056
What is the thermal output for a QIAsymphony SP and AS instrument?
FAQ ID -3057
How precise is the QIAgility?
FAQ ID -3063
What are the advantages and disadvantages of turning on the tip “re-use” feature on the QIAgility?
FAQ ID -3064
How long does it take to set up a 96-well plate PCR reaction on the QIAgility?
FAQ ID -3066
Can I use a bar code scanner or reader on the QIAgility?
FAQ ID -3067
How can I find the run parameters for a BioSprint protocol that I am running?
FAQ ID -3069
Can BioSprint 96 be connected to LIMS?
FAQ ID -3070
What is the expected level of endotoxins in plasmid DNA purified with QIAprep spin miniprep kit?
FAQ ID -3081
What is the expected level of endotoxins in plasmid DNA purified with QIAGEN Plasmid kits, QIAfilter Plasmid kits and HiSpeed Plasmid purification kits?
FAQ ID -3082
What is the concentration of the Gentra RNase A that is used with the Autopure and Puregene protocols?
FAQ ID -3083
What is the concentration of the different Proteinase K solutions sold by QIAGEN?
FAQ ID -3084
Where can I access Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit?
FAQ ID -3085
What tubes can be used with TissueLyser Adapter Set 2 x 96 (cat. no. 69984)?
FAQ ID -3086
How to upload a Flexiplate on QIAGEN Webpage?
FAQ ID -3103
Can we do modification XXXXX for custom siRNA that is not on the web page?
FAQ ID -3107
What is the stability of siRNA lyophilized and resuspended?
FAQ ID -3109
Do you offer FlexiTube or FlexiPlate siRNAs for rat?
FAQ ID -3114
Is it possible to extract RNA from frozen blood?
FAQ ID -3119
Is it possible to extract RNA from RNAprotect-stabilized-blood?
FAQ ID -3120
What's the pulsed-field gel electrophoresis (PFGE) conditions of the gel picture showing on your website? http://www.qiagen.com/Search/MagAttract-HMW-DNA-Kit#productdetails
-48
Can Dithiothreitol (DTT) be used instead of BME in Buffer RLT Plus of RNeasy Plus Mini Kit?
FAQ ID -3125
Why does my purified DNA/RNA sometimes have a 260/280 or 260/230 ratio of more than 2? Does that mean the purity is poor?
FAQ ID -3132
How do I remove the RNA contamination from purified DNA?
FAQ ID -3133
What is the final concentration of Protease upon reconstitution in the QIAamp DNA Blood Mini?
FAQ ID -3140
What is the Spike-in-Control in the miRNeasy Serum/Plasma Kit ?
FAQ ID -3169
What is the difference between the 1 nmol FlexiTube and FlexiTube Gene Solution siRNAs?
FAQ ID -3174
What could be a reason for cloudy precipitate in PCR reaction with STR Plus chemistry after cycling protocol is completed?
FAQ ID -3175
What is needed for performing delta delta Ct relative quantitation on the Rotor-Gene Q?
FAQ ID -3178
Is REST software Windows 7 compatible?
FAQ ID -3182
Can the Rotor-Gene Q perform gradient PCR?
FAQ ID -3183
What is the expected amplicon size of the Microbial DNA qPCR Assays?
FAQ ID — 3396
What is the difference between Positive PCR Control (PPC) and Microbial DNA Positive Control?
FAQ ID — 3397
What is LLOQ?
FAQ ID — 3401
What is the difference between LLOQ and LOD?
FAQ ID — 3402
What species are detected by the Pan Bacteria 1 and Pan Bacteria 3 Assays?
FAQ ID - 3427
What is the composition of Buffer AC in the QIAamp UltraSens Virus Kit?
FAQ ID - 3429
Can the miScript II RT Kit be used to quantify plant miRNAs?
FAQ ID - 3431
What is the dissociation curve temperature of PCR products generated with the miScript Plant qPCR system?
FAQ ID - 3444
Can specific types of white blood cells be enriched from a PAXgene Blood RNA Tube prior to the RNA isolation?
FAQ ID - 3467
What is the proper disposal procedure for waste from the sample preparation?
FAQ ID - 3470
What is the expected yield from 8.5 ml of blood?
FAQ ID - 3496
What does the resuspension buffer, Buffer BG4, in the PAXgene Blood DNA kit contain?
FAQ ID - 3501
How should blood samples drawn into PAXgene Blood DNA Tubes be transported?
FAQ ID - 3507
How long can samples in PAXgene Blood DNA Tubes be stored at room temperature?
FAQ ID - 3508
What is the expected DNA yield from 2.5 ml of blood when using the QIAsymphony, QIAcube, and salting-out precipitation method?
FAQ ID - 3510
Which downstream applications have been tested with DNA purified from samples collected in PAXgene Blood DNA Tubes IVD?
FAQ ID - 3512
What does PAXgene Tissue STABILIZER contain?
FAQ ID - 3513
What is the minimum number of cells that can be efficiently processed with the QIAamp DNA Mini and QIAamp Blood Mini kits and what is the expected yield?
FAQ ID - 3516
Is the quality and size of DNA extracted with the DNeasy Blood & Tissue kit good enough to generate DNA-libraries for next generation sequencing?
FAQ ID - 3517
What is the minimum number of cells or minimum amount of tissue that can be efficiently processed with the RNeasy mini kit?
FAQ ID - 3519
Can I use the RNeasy Fibrous Tissue Mini Kit (cat. no. 74704) to extract RNA from fibrous plant material?
FAQ ID - 3521
Will the Reverse transcription control on the RT2 profiler PCR array work on any cDNA library?
FAQ ID - 3534
Microscopic/visual confirmation of successfully sorted cells can be used to optimize sorting conditions. Can stained cells be used?
FAQ ID - 3559
Is the miRNA reverse transcription control (miRTC) used in conjunction with the miScript Single Cell qPCR Kit?
FAQ ID - 3573
Is clot detection possible on the QIAsymphony?
FAQ - ID 3588
How can I determine if a tube is suitable for clot detection on QIAsymphony?
FAQ ID - 3590
How should I thaw frozen plasma samples?
FAQ ID - 3645
How do Unique Molecular Indexes (UMIs) improve quantification?
FAQ ID - 3669
Can REPLI-g WGA be used to amplify cDNA if the cDNA is dsDNA (double-stranded DNA)?
FAQ ID - 3686
I would like to stop during the library preparation procedure. When can I do that?
FAQ ID - 3687
Which regions are covered by the GR DNAseq V2 Panels (#181900)?
FAQ ID - 3688
When preparing plasma with the PAXgene Blood ccfDNA tubes, cat. # 768115, do you recommend that the first centrifugation step should be done with the brakes on or off?
FAQ ID - 3690
How do I insert and replace an absorber strip into the PyroMark Q48 Autoprep chamber?
FAQ ID - 3691
Are peak heights of Q48 comparable to other PyroMark instruments?
FAQ ID - 3692
How much space does the PyroMark Q48 Autoprep instrument need?
FAQ ID - 3693
Can unused wells in a pyrosequencing discs be used in the next run?
FAQ ID - 3694
Where can I find the certificate of calibration for the Rotor-Disc OTV Kit (catalog number 981400)?
FAQ ID - 3695
What is the minimum guaranteed shelf-life of the PAXgene Blood ccfDNA Tubes (100) (catalog no. 768115)?
FAQ ID - 3696
What methods are used during normalization of RNAseq data in the secondary GeneGlobe Data Analysis Center?
FAQ ID - 3698
How do I prepare Buffer MP?
FAQ ID - 3620
Are the QIAshredder columns included in various QIAGEN kits the same?
FAQ ID - 3624
What is the composition of buffer QBT?
FAQ ID -411
What is the composition of buffer QC?
FAQ ID -412
What is the composition of buffer QF?
FAQ ID -413
What is the composition of buffer STE?
FAQ ID -415
What is the composition of buffer TE?
FAQ ID -416
What is the composition of buffer FWB2?
FAQ ID -417
What is the composition of buffer P3?
FAQ ID -418
What is the size, charge and isoelectric point of the DHFR protein in the pQE vectors?
FAQ ID -470
Why is carrier RNA used during the isolation of gDNA from microdissected samples with the QIAamp DNA Micro Kit?
FAQ ID -473
Why are the centrifugation speeds for the QIAamp DNA Mini kit at 6000 x g? Can I spin at full speed?
FAQ ID -474
Are HEPES, MOPS and PIPES buffers compatible with QIAGEN's PhosphoProtein Purification kit?
FAQ ID -482
Can Ni-NTA resins be used to purify protein with an internal His-tag?
FAQ ID -496
How do I calculate the percentage of silencing with real-time RT-PCR for siRNA?
FAQ ID -498
Do you have a protocol for the removal of endotoxins from already purified plasmid DNA?
FAQ ID -500
Do you have stability data for genomic DNA isolated with the QIAamp DNA Blood Mini Kit?
FAQ ID -518
What is the composition of the siRNA resuspension & annealing buffer?
FAQ ID -522
Can Taq DNA Polymerase use RNA as a template, and generate false positives in "no-RT" controls?
FAQ ID -523
What is Tissue-Tek O.C.T., and what is it used for?
FAQ ID -530
Why do replicates in real-time PCR have different plateau heights?
FAQ ID -539
How can I avoid primer-dimer formation during PCR amplification?
FAQ ID -544
How can I tell if I have primer-dimers in my PCR reaction?
FAQ ID -552
How should I store cDNA produced using Omniscript Reverse Transcriptase?
FAQ ID -561
Is it possible to isolate DNA from bone marrow with the QIAamp DNA Blood Kits?
FAQ ID -563
What is the composition of Buffer ER? Is it available separately?
FAQ ID -571
What can I do when the DNA pellet prepared with QIAGEN Plasmid Kits has been overdried?
FAQ ID -572
Do I have to remove the oil from my PCR reaction before using the QIAquick or MinElute PCR Purification Kit?
FAQ ID -575
Are the columns of the QIAquick PCR Purification-, Gel Extraction-, and Nucleotide Removal Kit interchangeable?
FAQ ID -577
What size is the smallest protein that can be synthesized with the EasyXpress Protein Synthesis Kit?
FAQ ID -600
Why do I sometimes get light blue colonies when using the QIAGEN PCR Cloning Kit?
FAQ ID -603
What do you recommend for the cleanup of genomic DNA (gDNA)?
FAQ ID -618
How can I improve my RNA yield from liver sample when using RNeasy Kits?
FAQ ID -623
Why do you recommend using Triton X for the purification of 6xHis-tagged protein?
-100
What 96-well plates do you recommend for use with the LiquiChip Workstation?
FAQ ID -630
What is a QIAshredder? Is it sufficient for complete disruption and homogenization of my tissue sample?
FAQ ID -631
Why does the QIAamp DNA Mini Tissue Protocol require both ATL and AL buffer, while the Blood Protocol only uses AL?
FAQ ID -633
Will the QIAquick PCR Purification Kit remove sufficient SYBR Green from real-time PCR reactions to allow sequencing?
FAQ ID -637
What is the shelf-life of my QIAGEN Kit?
FAQ ID -651
What are the differences between MDA and DOP/PEP methods of Whole Genome Amplification?
FAQ ID -665
Can I purchase Phi29 DNA polymerase only?
FAQ ID -708
What is the average DNA concentration obtained using the DirectPrep 96 Miniprep Kit?
FAQ ID -721
I accidentally stored Buffer RDD of the RNase-Free DNase Set at°C. Will it still function?
-20
I ran my RNA out on an agarose gel and can see lots of bands similar to a ladder. Why?
FAQ ID -745
How much DNA is obtained in the average PCR reaction?
FAQ ID -750
Do you have information about the cleanup of single-stranded DNA (ssDNA) with QIAquick columns?
FAQ ID -759
How can I get a certificate for quality assurance (ISO 13485 and ISO 9001) from QIAGEN?
FAQ ID -762
What is the difference between Ni-NTA Agarose and Ni-NTA Superflow?
FAQ ID -764
What is the composition of Buffer N3?
FAQ ID -767
Are the protocols from the BioSprint software compatible with the KingFisher software and vice versa?
FAQ ID -770
How should fluorescent labeled probes be stored?
FAQ ID -784
Is it possible to use the QuantiTect Reverse Transcription Kit with bacterial RNA?
FAQ ID -785
Are Buffer PB of the QIAquick PCR Purification Kit and Buffer QG of the QIAquick Gel Extraction Kit interchangeable?
FAQ ID -786
How can I get specific information and a quote for my individual Sequencing Service needs?
FAQ ID -792
Can the QIAprep Spin Miniprep Kit be used for isolating plasmid DNA from mammalian cells?
FAQ ID -795
How can I separate PCR fragments that are small and very close in size on an agarose gel?
FAQ ID -800
Can I reuse the Ni-NTA Agarose and Ni-NTA Superflow resins?
FAQ ID -802
What Disinfectant Solution do you recommend for the BioRobot MDx?
FAQ ID -809
Is the E. coli Host Strain M15[pREP4] resistant to Zeocin?
FAQ ID -842
What annealing temperature should be used with the QuantiTect Primer Assays?
FAQ ID -849
Why is it recommended to add 1 mM IPTG for optimal protein yields using the EasyXpress Protein Synthesis Kits?
FAQ ID -860
Which QIAGEN plasmid preparation kits will contain LyseBlue Reagent?
FAQ ID -865
Do you have an in vitro translation kit allowing the incorporation of seleno-methionine for X-Ray crystallography based protein analysis?
FAQ ID -879
Do you have a protocol for the isolation of genomic DNA from frozen clotted blood?
FAQ ID -900. Test
Do you have protocols for the isolation of genomic DNA from tissue using QIAGEN-tips 2500 (Mega) and 10000 (Giga)?
FAQ ID -907
Do you have a protocol for the isolation of genomic DNA from bone?
FAQ ID -908
What do you suggest to prevent degradation of RNA isolated from tissue with high amounts of RNases using RNeasy?
FAQ ID -942
Do you have a protocol for Cyanine 570, Cyanine 670, or biotin labeling of cDNA?
FAQ ID -959
Do you have a protocol for reverse transfection of adherent cells with siRNA in 384-well plates?
FAQ ID -971
Do you have a protocol for the purification of PCR products using the BioSprint System?
FAQ ID -984
Do you have a protocol for transient transfection of Hela-S3 cells using PolyFect Transfection Reagent?
FAQ ID -997
How can I analyze proteins from exosomes and other extracellular vesicles (EVs) obtained with the exoEasy Maxi Kit by SDS-PAGE?
FAQ ID - 3707
How can I increase expression of my 6xHis-tagged protein in E. coli?
FAQ ID -63
Can miRNA and other RNAs smaller than 200 nucleotides be isolated with the AllPrep DNA/mRNA Nano Kit?
148723
What is the minimum amount of starting material that can be used with the DNA/mRNA Nano Kit?
148724
What is the composition of buffer OW2?
FAQ ID -419
What is the composition of buffer OBB?
FAQ ID -421
Do I need to buy special kits for the QIAcube Connect MDx?
148725
What applications are offered on the QIAcube Connect MDx instrument?
148726
Can I program my own protocols for the QIAcube Connect MDx?
148727
Can purification columns from other suppliers be processed on the QIAcube Connect MDx?
148728
What are the dimensions and weight of the QIAcube Connect MDx?
148729
Can the QIAcube rotor adapters be autoclaved?
148730
Can the QIAcube rotor and/or buckets be removed for cleaning?
148731
Can the QIAcube be connected to a laboratory information management system?
148732
Are run reports and/or log files available on the QIAcube?
148733
How can I get software updates for the QIAcube Connect MDx?
148734
Can QIAcube Connect MDx protocol runs be interrupted, and subsequently be continued to completion?
148735
Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube?
148736
Do I need to discard partially used QIAcube tip racks?
148738
How can I decontaminate the QIAcube Connect MDx system?
148739
How can I load new protocols onto the QIAcube Connect MDx?
148740
Can the QIAcube Connect MDx heater/shaker be used independently from protocol runs?
148741
Can the QIAcube Connect MDx centrifuge be used independently from protocol runs?
148742
What dedicated QIAcube Kits are available?
148743
Can I ask QIAGEN to upgrade my current QIAcube Connect Life Science instrument to make it an MDx instrument?
148744
Can I run life science and custom protocols on a QIAcube Connect MDx?
148745
What are the heating/cooling rates in the QIAquant instruments?
153919
Is a passive internal reference dye, like ROX, required on the QIAquant to obtain reproducible results?
153920
What sample volume is suitable for use in the QIAquant?
153921
What types of reaction vessels are required for use in the QIAquant?
153922
Is the QIAquant equipped with a temperature gradient function?
153923
Are the 96- and 384-sample blocks interchangeable?
153924
Is it possible to use the touch down PCR option on QIAquant with two acquisition points in the same channel?
153925
How many molecules can be detected in the system?
153926
What applications can the QIAquant be used for?
153927
Can I upgrade my QIAquant 96 2plex instrument to QIAquant 96 5plex or modify the set of color channels?
153928
Does QIAGEN offers arrays and assays configured specifically for QIAquant instrument?
153929
Is the software of the QIAquant compatible with 21CFR part11?
153930
Is it possible to import a standard curve from a previous run?
153947
Can data be analyzed while the run is proceeding? Can data be analyzed from a previous experiment while another experiment is running?
153948
Can I use QIAquant 96 Software to analyze experiments carried out on QIAquant 384?
153950
Can I operate QIAquant 96 instrument using touchscreen and computer simultaneously?
153951
Is it possible to establish an internet connection with QIAquant instrument?
153952
Can I connect more than one QIAquant instrument to the same computer?
153953
What is the algorithm behind data normalization in the QIAquant Software?
153954
Is it possible to edit the sample sheet after the run in the QIAquant Software?
153955
What are the LIMS capacity of the software?
153949
Does the QIAquant Software have the capacity to export data? Which data formats can be used for the export?
153946
I received a kit containing the MinElute columns; however, they were left out for a while and not stored at 2–8°C upon receipt. Can I still use them?
FAQ ID - 3560
Is the DNA extracted with the QIAamp DNA FFPE Advanced Kits suitable for downstream applications that require more intact DNA such as long range PCR (>1 kb) and long read DNA sequencing?
153891
What are recommended stopping points in the procedure of the QIAamp DNA FFPE Advanced Kits?
153892
Can samples be lysed overnight with Proteinase K in the QIAamp DNA FFPE Advanced procedure?
153893
Can the second Proteinase K lysis step in the QIAamp DNA FFPE Advanced procedure be carried out at lower temperatures than 65°C?
153894
Can the 2nd Prot K step be omitted?
153896
What size of DNA can be expected?
153897
What is the difference of buffer FTB versus Buffer ATL?
153895
Is it possible to change voltage set-up from 110V to 230V on the QIAcuity instruments?
3761
Can I see error codes on the instrument touchscreen?
3763
What is the scope of a regular maintenance of the QIAcuity?
3765
What is the impact of not applying the latest VPF? Can I reanalyze previously obtained results after installing the latest VPF?
3769
Can I see on the QIAcuity Software Suite report file if the VPF (Volume Precision Factor) has been used or not?
3770
Is it necessary to reanalyze a plate with VPF (Volume Precision Factor) that was already processed using a QIAcuity instrument that was purchased in 2020?
3771
Can I prepare a dPCR reaction directly in QIAcuity Nanoplate?
3774
How to prepare DNA prior to dPCR?
3778
Can I use a custom master mix instead of a QIAGEN master mix?
3777
What are the storage conditions and expiry date of QIAcuity consumables?
3780
Can I use the QIAcuity Nanoplate in more than one runs?
3781
What is the VPF (Volume Precision Factor)?
3784
When and how often do I need a new VPF (Volume Precision Factor)?
3785
Is a standard curve needed in dPCR?
3786
Which database versions are used for CRISPR-Q Custom PCR Assay and CRISPR-Q Sanger Primers design?
3788
Is there anything to consider when processing cultured cells on coated cultivation dishes? Are additional purification steps required to eliminate or reduce coating reagents such as Poly-L-Lysine?
3791
Is there something to consider when working with transduced cells treated with Polybrene?
3790
For sequencing application, does the lysate require purification?
3792
Are the CRISPR-Q PCR Assays and the CRISPR-Q Sanger Primers on GeneGlobe validated?
3793
Are the PCR products generated with CRISPR-Q Custom PCR Assays only applicable to analysis of editing efficiency by Sanger sequencing?
3794
Can the new CRISPR PCR Assays be used for dPCR on the QIAcuity? Do you have data or a protocol?
3795
What is the minimum cell number needed for cell lysis?
3789
For the Sanger analysis tool, the customer needs to upload two .abi1 files of forward and reverse sequences? Is there any additional info that needs to be provided?
3796
What is the sequence of the QIAseq miRNA NGS 5’ Adapter?
FAQ ID - 3672
What are recommended stopping points in the procedure of the EZ1&2 DNA FFPE Kits?
3753
Are there other options for paraffin removal?
3754
Can samples be lysed overnight with Proteinase K in the EZ1&2 DNA FFPE procedure?
3755
Can the second Proteinase K lysis step in the EZ1&2 DNA FFPE procedure be carried out at lower temperatures than 65°C?
3756
What is the difference between Buffer FTB and Buffer ATL?
3757
Can the 2nd Proteinase K step be omitted?
3758
What size of DNA can be expected?
3759
Is the DNA extracted with the EZ1&2 DNA FFPE Kits suitable for downstream applications that require more intact DNA such as long-range PCR (>1 kb) and long-read DNA sequencing?
3760
Do you recommend 1-step or 2-step real-time RT-PCR for gene expression analysis?
FAQ ID -1056
3323 - Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube?
FAQ ID - 3323

 

Does salt concentration in the food matter when using the DNeasy mericon Food Kit?
FAQ ID - 3346
Why does the DNeasy mericon Food Kit use a QIAquick column and Buffer PB rather than a DNeasy column and Buffer AL, which might be expected since the kit isolates genomic DNA?
FAQ ID - 3347
When would I use the mericon DNA Bacteria Kit vs the mericon DNA Bacteria Plus Kit?
FAQ ID - 3348
Why is my 260/280 ratio low after using the DNeasy mericon Food Kit?
FAQ ID - 3349
CAn I use milk or other liquids with the DNeasy mericon Food kit? What volume would I use?
FAQ ID - 3350
What is the composition of QIAzol? What is the color of this reagent?
FAQ ID - 3355
Is it possible to stop the DNeasy tissue protocol and store the tissue lysates after digesting in buffer ATL and Proteinase K?
FAQ ID - 3362
I cannot analyze the data from your Investigator HID kits with my GeneMapper software. What can I do?
FAQ ID - 3363
What is the QIAxcel QX RNA Size Marker 200-6000nt?

FAQ ID - 3365

What is the nature of the gel in the QIAxcel RNA QC kit v2.0? Is it denaturing?
FAQ ID - 3366
Do you sell CoralLoad dye separately?
FAQ ID - 3368
Are QIAcube reagent bottles (cat. no. 990393) autoclavable? What type of plastic are they made of?
FAQ ID - 3369
How long can samples in PAXgene Blood DNA Tubes (IVD) be stored at 2–8°C?
FAQ ID - 3379
Which downstream applications have been tested with DNA purified from samples collected in PAXgene Blood DNA Tubes (IVD)?
FAQ ID - 3384
What is the composition of elution buffers used in QIAsymphony DNA Investigator kits?
FAQ ID - 3387
Can I buy the RNeasy Mini columns, RNeasy MinElute columns, RNeasy Midi columns or the RNeasy Maxi columns separately?
FAQ ID - 3388
Can I buy the gDNA eliminator plates supplied in your RNeasy Plus 96 kit separately?
FAQ ID - 3389
Can I buy the gDNA eliminator columns supplied in your RNeasy Plus kits separately?
FAQ ID - 3390
Why does AllPrep DNA/RNA/Protein Mini kit use buffer RLT, but Allprep DNA/RNA Mini and Allprep DNA/RNA Micro kits use buffer RLT Plus? Are these buffers interchangeable?
FAQ ID - 3391
Do you have data to show lowest dropout rate with Repli-G WTA Single Cells kit?
FAQ ID - 3392
What is the RNase A concentration and composition of Buffer P1?
FAQ ID -198
Are protocols also available for other instruments (e.g., Hamilton Star and Tecan Fluent)?
3797
Where can I download the protocol for the KingFisher Flex?
3798
Who can help in case of issues with the third party instrument?
3799
Is the virus inactivated during the procedure?
3800
Can I use sample volumes other than 300 µl?
3801
Who can help if it is not clear if issues are caused by the third party instrument or by the chemistry?
3802
I would like to use the kit on another third party instrument. Who should I contact?
3803
How long does a saliva collection take?
3804
What can I do to stimulate saliva flow?
3805
Can I stop saliva collection before the fill line is reached?
3806
What can I do if I don't see the fill line anymore due to saliva bubbles?
3807
What happens if I accidentally unscrew the funnel before saliva collection is completed?
3808
May I continue with the saliva collection if I accidentally unscrewed the funnel prematurely?
3809
What is in the additive in the PAXgene Saliva Collector?
3810
What is the shelf life of unused PAXgene Saliva Collectors?
3811
How should unused PAXgene Saliva Collector be stored?
3812
What should I avoid before sample collection?
3813
How does the stabilizing reagent in the PAXgene Saliva Collector stabilize saliva?
3814
How long can saliva in PAXgene Saliva Collector tubes be stored at room temperature?
3815
Can saliva in PAXgene Saliva Collector tubes be stored at temperatures higher than 25°C?
3816
Can saliva in PAXgene Saliva Collector tubes be stored frozen at –20 or –80°C?
3817
Is RNA preserved in saliva collected in PAXgene Saliva Collector?
3818
Are pathogens inactivated in human saliva samples collected with the PAXgene Saliva Collector?
3819
What should I do if stabilized saliva samples are very heterogenous?
3820
What is the expected DNA purity from saliva collected and stabilized with the PAXgene Saliva Collector?
3823
Is it possible to process volumes of saliva from the PAXgene Saliva Collector other than the 400 or 1000 µl from the standard protocols for the QIAsymphony DNA Midi Kit using the QIAsymphony SP instrument?
3824
Is it possible to purify DNA from the whole sample of a PAXgene Saliva Collector in one preparation?
3826
Which downstream applications have been tested with DNA purified from saliva collected into PAXgene Saliva Collector?
3827
Which downstream applications have been tested with SARS-CoV-2-derived RNA purified from saliva collected into PAXgene Saliva Collector?
3828
Is a restriction digest necessary for DNA isolated from PAXgene Saliva samples before performing a dPCR run using the QIAcuity?
3829
Is it possible to place the PAXgene Saliva Collector tube directly on the QIAsymphony SP instrument sample rack?
3825
What is the expected DNA yield per milliliter saliva collected and stabilized with the PAXgene Saliva Collector?
3822
Which QIAGEN DNA extraction kits are compatible with the PAXgene Saliva Collector?
3821
What are common sized libraries observed on a TapeStation, Bioanalyzer, or similar instruments?
FAQ ID - 3837
Are the QIAseq miRNA NGS 5’ Adapter and QIAseq miRNA NGS RT Initiator compatible with the QIAseq miRNA UDI kits?
FAQ ID - 3836
What is the sequence of the UDI 5' Adapter?
FAQ ID - 3835
Can you perform qPCR analysis of miRNAs from libraries prepared with the QIAseq miRNA Library Kit?
FAQ ID - 3674
What can be used to validate results obtained with the QIAseq miRNA Library Kit?
FAQ ID - 3675
What is the maximum number of available sample indexes?
FAQ ID - 3665
What are recommended stopping points during the QIAseq miRNA Library Kit procedure?
FAQ ID - 3663
Should total RNA or small enriched RNA be used as the starting material for the QIAseq miRNA Library Kit?
FAQ ID - 3659
What is the recommended read length for libraries prepared using the QIAseq miRNA Library Kit?
FAQ ID - 3668
What is the acceptable number of reads per sample per miRNA sequencing run?
FAQ ID - 3673
Do you have a protocol for the isolation of genomic DNA from whole blood for use in MRC-Holland MLPA® assays?
FAQ ID -1169
Is training of lab personnel included with the purchase of a BioRobot EZ1?
FAQ ID -1239
Do the foils sealing the EZ1 Reagent Cartridges have to be manually removed prior to loading the robot?
FAQ ID -1240
How can we get our BioRobot EZ1 upgraded when new protocols are launched?
FAQ ID -1241
Where can I find information about the worktable setup on the BioRobot EZ1?
FAQ ID -1243
What disposables are required for nucleic acid isolations on the BioRobot EZ1?
FAQ ID -1246
How should I prepare buffy coat samples for use on the BioRobot EZ1?
FAQ ID -1249
Do you have a protocol for the isolation of DNA from buffy coat using the BioRobot EZ1?
FAQ ID -985
What has to be done to an RNA sample before loading it onto an Agilent Bioanalyzer?
FAQ ID -528
Do you have a protocol for the isolation of total nucleic acids from animal and human tissues on the BioRobot EZ1?
FAQ ID -975
Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the BioRobot EZ1?
FAQ ID -976
Do you have a protocol for the isolation of DNA from fungi using the BioRobot EZ1?
FAQ ID -992
Are the new EZ1 RNA Kits (cat. no. 958034; 959034; 956034) compatible with the previous EZ1 RNA Card (cat. no. 9015590) or EZ1 RNA Universal Tissue Card (cat. no 9016384)?
FAQ ID -1025
What are the advantages of EnzScript M-MLV Reverse Transcriptase RNase H-(P7600) versus wild type M-MLV Reverse Transcriptase (P7040)?
FAQ ID - 3838
What types of priming are compatible with EnzScript M-MLV Reverse Transcriptase RNase-H-?
FAQ ID - 3839
What is the optimal reaction temperature for EnzScript M-MLV Reverse Transcriptase RNase H-?
FAQ ID - 3840
What PCR enzymes can be used following the first-strand synthesis?
FAQ ID - 3841
What is the suggested protocol for generating long, full-length cDNA transcripts (>5 kb)?
FAQ ID - 3842
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