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How is an antibody epitope determined?
FAQ ID -593
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Do I need to calibrate my real-time cycler if I want to use Yakima Yellow?
FAQ ID -540
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Do we recommend pooling siRNA sequences?
FAQ ID -517
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Does the His-tag interfere with the purification procedure of the QIAGEN PhosphoProtein Purification Kit?
FAQ ID -598
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Does the use of MAPK1 as a control in siRNA transfection experiments have secondary effects on the cell culture?
FAQ ID -548
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Do you have a protocol to isolate RNA from blood and bone marrow using the BioRobot EZ1?
FAQ ID -567
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Do you have any recommendations for using the PosphoProtein Kit with starting material other than cells?
FAQ ID -597
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Do you have a protocol for isolating RNA from yeast using RNeasy?
FAQ ID -535
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What is the run time for the BioSprint 96 DNA Blood protocol on the BioSprint 96 instrument?
FAQ ID -622
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Will REPLI-g work at high temperatures?
FAQ ID -656
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The Multiflow Dispense Head on my BioRobot is not dispensing the correct amount of liquid. What can I do?
FAQ ID -652
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What is the smallest amount of DNA I can put into a 50 ul REPLI-g reaction?
FAQ ID -663
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What is the recommended incubation time to stabilize tissue RNA in RNAprotect Tissue Reagent?
FAQ ID -647
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What do you mean by “Quantification of locus representation” in REPLI-g WGA product?
FAQ ID -698
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What is REPLI-g whole genome amplification?
FAQ ID -654
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What is the average amount of DNA and RNA present in 1 ml normal serum?
FAQ ID -635
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What is the biggest protein that can be synthesized with the EasyXpress Protein Synthesis System?
FAQ ID -601
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What is the length of the MDA whole genome amplification product?
FAQ ID -690
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What is the expected yield of genomic DNA isolated from bacteria with the DNeasy Blood & Tissue or QIAamp DNA Mini Kit?
FAQ ID -632
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Is it possible to perform laser microdissection with tissues stabilized with RNAprotect Tissue reagent?
FAQ ID -608
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Is it possible to synthesize membrane proteins using the EasyXpress Protein Synthesis Kit?
FAQ ID -602
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What are Elution Microtube adapters and Microplate adapters used in the DirectPrep 96 Miniprep Kit, and how can I order them?
FAQ ID -646
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What are the advantages of REPLI-g over conventional DNA sample processing and amplification methods?
FAQ ID -686
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Why do I get amplification in a negative control DNA tube using the REPLI-g Kit for WGA?
FAQ ID -675
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Why did you choose MAPK1 as a positive control in the QIAGEN RNAi Human/Mouse Control Kit?
FAQ ID -604
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Will the PhosphoSerine antibody recognize Serine residues if the neighboring residues are Leucine or Isoleucine?
FAQ ID -644
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Will TA cloning work with REPLI-g WGA product?
FAQ ID -672
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Will DNA from chromosomes collected in TE with a drop of mineral oil work with REPLI-g amplification?
FAQ ID -664
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What is the stability of the REPLI-g MDA product?
FAQ ID -693
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When delivering liquid to a 96-well plate using the MultiFlow Dispenser Head on my BioRobot , one or more rows in the plate contains less liquid per well than specified by the protocol. What is going wrong?
FAQ ID -653
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Where should I add a dye-label modification on the siRNA?
FAQ ID -648
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What types of materials from environmental samples could interfere with WGA?
FAQ ID -666
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Can I stabilize RNA in bacteria grown on solid media or substrate using the RNAprotect Bacteria Reagent?
FAQ ID -615
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Can I re-amplify whole genome amplification product?
FAQ ID -670
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Can I use another lysis buffer than the one supplied in the PhophoProtein Purification Kit?
FAQ ID -645
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Can I run less than 15 samples on the BioSprint 15 instrument?
FAQ ID -621
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Can the QIAshredder Maxi Spin Columns from the DNeasy Plant Maxi Kit be used for RNeasy samples?
FAQ ID -616
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Do you have an off-the shelf diagnostics kit for WGA?
FAQ ID -678
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Are there components in biological samples that can inhibit the REPLI-g reaction?
FAQ ID -667
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Are the BioSprint 15/96 instruments stand-alone workstations?
FAQ ID -620
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Can the computer for the LiquiChip Workstation be networked?
FAQ ID -629
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Are QIAGEN EZ Competent Cells of the QIAGEN PCR Cloning Kit dam+ or dam-?
FAQ ID -638
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Are samples that are contaminated with high molecular weight DNA (e.g. from a paramedic or police officer) a problem with REPLI-g amplification?
FAQ ID -683
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Can BAC DNA prepared with the R.E.A.L. Prep 96 Plasmid Kit be used for high-throughput sequencing?
FAQ ID -643
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Can I do whole genome amplification from brain tissue using REPLI-g?
FAQ ID -669
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Can I do whole genome amplification from mitochondrial DNA using REPLI-g?
FAQ ID -668
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Can FTA paper for dried blood spots be used with REPLI-g?
FAQ ID -685
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How can I quantify the amount of REPLI-g DNA I have amplified?
FAQ ID -694
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Can I amplify from plasma or serum samples using the REPLI-g Mini/Midi kits?
FAQ ID -671
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How should I store the REPLI-g amplification product?
FAQ ID -692
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Is 2 kb the minimal gDNA fragment size for REPLI-g Whole Genome Amplification?
FAQ ID -682
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How long can I store bacterial culture stabilized in RNAprotect Bacteria Reagent?
FAQ ID -614
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Do I need to clean up the REPLI-g amplification product for downstream analysis?
FAQ ID -691
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Do you have a kit for RNA isolation from any kind of sample type?
FAQ ID -627
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Does REPLI-g technology for whole genome amplification work with paraffin embedded samples?
FAQ ID -673
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How can I determine the quality of my REPLI-g amplified products?
FAQ ID -695
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How can I decrease ribosomal RNA (rRNA) contamination using Oligotex?
FAQ ID -642
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Do you know of anything in the buffers/DNA sample that can cause bias in REPLI-g WGA?
FAQ ID -684
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Do you have a protocol for the purification of DNA from rodent tail using the BioSprint System?
FAQ ID -979
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Do you have a protocol for the isolation of DNA from epithelial cells mixed with sperm cells?
FAQ ID -926
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Do you have a protocol for isolation of genomic DNA from saliva using the DNeasy Blood & Tissue Kit?
FAQ ID -931
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Do you have a protocol for purification of DNA from clotted blood using the FlexiGene DNA Kit?
FAQ ID -973
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Do you have a protocol for Streptokinase treatment of clotted blood?
FAQ ID -912
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Do you have a protocol for the isolation of total nucleic acids from animal and human cells on the BioRobot EZ1?
FAQ ID -974
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Do you have a protocol for the isolation of total RNA from plant tissue using QIAGEN-tips?
FAQ ID -949
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Do you have a protocol for the isolation of total RNA from buccal swabs?
FAQ ID -956
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Do you have a protocol for the isolation of viral DNA from stool?
FAQ ID -929
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Do you have a protocol for the isolation of total RNA from plants using the RNeasy 96 Kit?
FAQ ID -951
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Do you have a protocol for the isolation of DNA from formalin-preserved stool samples?
FAQ ID -922
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Do you have a protocol for the isolation of genomic DNA from fungi?
FAQ ID -911
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Do you have a protocol for the isolation of genomic DNA and/or proteins from fatty tissue treated with QIAzol?
FAQ ID -955
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Do you have a protocol for the purification of DNA from cells using the BioSprint System?
FAQ ID -977
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Do you have a protocol for the purification of DNA from dried blood spots using the BioSprint System?
FAQ ID -982
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Do you have a protocol for the preparation of tissues preserved in RNAprotect Tissue Reagent for pathological studies?
FAQ ID -950
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Do you have a protocol for the purification of DNA from buccal swabs using the BioSprint System?
FAQ ID -983
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Do you have a protocol for isolation of genomic and viral DNA from lymphocytes using QIAGEN Genomic-tips?
FAQ ID -901
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Do you have a protocol for isolation of genomic and plasmid DNA from cell cultures using QIAGEN Genomic-tips?
FAQ ID -902
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Do you have a protocol for transfection of adherent cells with low concentrations of siRNA using RNAiFect Transfection Reagent?
FAQ ID -970
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Do you have a protocol for GMO testing of food samples?
FAQ ID -921
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Do you have a protocol for isolation of bacterial DNA from soil?
FAQ ID -923
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Do you have a protocol for isolation of genomic DNA from dried blood spots using the QIAamp 96 DNA Blood Kit?
FAQ ID -919
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Do you have a protocol for isolation of genomic DNA from insects?
FAQ ID -904
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Do you have a protocol for detection of HBV DNA by PCR?
FAQ ID -915
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Does QIAGEN sell a vector with a T7 promotor for use with the EasyXpress Protein Synthesis Insect Kit?
FAQ ID -937
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Can the BioSprint instruments be placed in a cool room?
FAQ ID -938
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Do you have a protocol for Acetyl Cysteine (NALC) treatment of viscous samples?
FAQ ID -913
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Do you have a protocol for co-transfection of adherent cells with siRNA and plasmid DNA?
FAQ ID -969
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Do you have a protocol for adding new reporter dyes to the ABI PRISM 7900 Sequence Detection System?
FAQ ID -965
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Do you have a protocol for cleanup of non-radioactive DNA samples?
FAQ ID -945
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Do you have a protocol for radioactive labelling of cDNA?
FAQ ID -960
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Do you have protocols for multiple extractions of DNA fragments from agarose gels?
FAQ ID -944
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Why is it not recommended to stabilize cells with RNAprotect Tissue Reagent?
FAQ ID -941
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Do you have a protocol for the detection of Bordetella pertussis DNA by PCR?
FAQ ID -914
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Do you have a protocol for the isolation of DNA from cultured cells using the BioRobot EZ1?
FAQ ID -990
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Do you have a protocol for performing quantitative multiplex PCR on the LightCycler 2.0 system?
FAQ ID -966
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Do you have a protocol for long-term, siRNA-mediated gene silencing using HiPerFect Transfection Reagent?
FAQ ID -972
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Do you have a protocol for manual purification of 6xHis-tagged proteins expressed in E. coli using Ni-NTA Superflow?
FAQ ID -967
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Do you have a protocol for purification of DNA from cultured animal cells using the DNeasy 96 Blood & Tissue Kit?
FAQ ID -934
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Do you have a protocol for purifying 2x96 PCR samples simultaneously with the QIAquick 96 PCR Purification Kit?
FAQ ID -935
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Do you have a protocol for purification of DNA fragments from Cy3/Cy5 dye-label reactions?
FAQ ID -947
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Do you have a protocol for polyacrylamide gel analysis of oligonucleotides?
FAQ ID -961
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Do you have a protocol for isolation of genomic DNA from plants and filamentous fungi using QIAGEN Genomic-tips?
FAQ ID -905
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Do you have a protocol for isolation of genomic DNA from flies using QIAGEN Genomic-tips?
FAQ ID -903
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What are exo-resistant random hexamers used in the REPLI-g reaction?
FAQ ID -710
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The Sigma Centrifuge makes a loud noise at low speeds which disappears at higher speeds, when using the 2x96-well plate rotor. What is the problem?
FAQ ID -776
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Is RNAprotect Bacteria Reagent compatible with the RNeasy Mini Kit?
FAQ ID -797
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What is the basic technology behind the Strep-tag Protein Purification System?
FAQ ID -740
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What are the expected average DNA yields from plant extractions on the BioSprint 96?
FAQ ID -771
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If template abundance differs significantly between housekeeping and target gene, are both amplified equally efficient with the QuantiTect Multiplex Kits?
FAQ ID -718
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How to quantitate DNA purified using the BioSprint 96?
FAQ ID -773
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How to store DNA from forensic samples eluted into water on the EZ1 biorobot in TE buffer instead?
FAQ ID -734
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Is it possible to use LiquiChip beads for FACS applications?
FAQ ID -733
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Is it possible to buy the EZ Competent Cells of the QIAGEN PCR Cloning Plus Kit separately?
FAQ ID -763
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Is GFP excited and detected by the green laser of the LiquiChip System?
FAQ ID -737
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Which Qproteome or Protein Fractionation Kits from QIAGEN are compatible with tissue samples?
FAQ ID -755
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Why are some of my RNAprotect-stabilized samples frozen at 0ºC while others are not?
FAQ ID -758
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Why are the QuantiTect and QuantiFast Multiplex PCR Kits limited to triplex real-time PCR on some cyclers?
FAQ ID -715
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Why should DNA or cDNA targets be less than 250 bp long for real-time PCR?
FAQ ID-751
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Where can I find info on compatible reporter dyes for use in real-time multiplex PCR using the QuantiTect Multiplex PCR Kits?
FAQ ID -719
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Why might my gene of interest drop out during WGA if it is not near a telomere or centromere?
FAQ ID -703
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Will the random hexamers in the REPLI-g reaction interfere with downstream analysis?
FAQ ID -713
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Why is there no detection of free reporter fluorescence in the LiquiChip System?
FAQ ID -732
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What is the cell number range one can use with the FastLane Cell cDNA Kit for real-time RT-PCR?
FAQ ID -782
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What negative (unphosphorylated) and positive (phosphorylated) control proteins do you recommend for use with the PhosphoProtein Purification Kit?
FAQ ID -779
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What is the enzyme used in the REPLI-g reaction?
FAQ ID -704
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What type of Sequencing Services does QIAGEN offer?
FAQ ID -781
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Where can I find a copy of the report files generated by running accessory protocols "Dilutor Delivery (monthly)" and "High-Speed Pipetting Sytem (monthly)" on my BioRobot 9604?
FAQ ID -766
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What is the general sensitivity level of the LiquiChip System?
FAQ ID -731
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Can QIAGEN's Taq- and HotstarTaq DNA Polymerases be used for cycle sequencing?
FAQ ID -741
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Can I use the PhosphoProtein Purification Kit to purify phosphorylated peptides of around 1 kD size?
FAQ ID -778
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Can RNAprotect Tissue Reagent be used to stabilize tissue which has already been flash-frozen?
FAQ ID -757
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Can lyophilized plant material be used for DNA extraction on the BioSprint 96?
FAQ ID -772
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Can the REPLI-g WGA enzyme work with labeled dNTPs?
FAQ ID -706
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Can the MAPK1 siRNA of the RNAi Human/Mouse Starter Kit be purchased separately?
FAQ ID -744
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Can the QIAvac 96 vacuum manifold be used with DirectPrep 96 plates?
FAQ ID -722
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Are the buffers in the Ni-NTA Fast Start Kit the same as the ones for use with Ni-NTA purchased separately?
FAQ ID -791
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Any data on the fidelity of the REPLI-g MDA technique?
FAQ ID -707
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Are Centromeres and Telomeres amplified using REPLI-g WGA?
FAQ ID -702
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Can I use my own primers for REPLI-g WGA to amplify a specific chromosomal region?
FAQ ID -712
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Can I do 4-plex real-time PCR, on the ABI PRISM 7000, 7700, or 7900 using the QuantiTect Multiplex PCR Kit?
FAQ ID -716
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How can one perform multiplex real-time PCR analysis using the LightCycler 2.0?
FAQ ID -780
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How can I be sure that I am harvesting my induced bacterial culture at the best time point for protein expression?
FAQ ID -788
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How do the FastLane Cell cDNA Kit and QuantiTect Reverse Transcription Kit eliminate genomic DNA contamination?
FAQ ID -783
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How many cells can be used with the FastLane Cell cDNA Kit?
FAQ ID -796
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Do you have an antibody for Western Blot Analysis of MAPK1 gene knockdown using your MAPK1 control siRNA?
FAQ ID -746
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Can frozen blood be used for whole genome amplification with REPLI-g?
FAQ ID -794
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Do I have to switch off the laser of the LiquiChip Workstation overnight?
FAQ ID -736
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How many reporter dyes can be used in the same analysis using the LiquiChip Workstation?
FAQ ID -738
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Do I need to determine limiting primer concentrations with the QuantiTect Multiplex PCR Kits?
FAQ ID -714
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Do you synthesize thiophosphate modified random hexamers?
FAQ ID -711
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Has anyone verified whole genome amplification accuracy with Sequencing?
FAQ ID -701
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Do you have a protocol for the fixation of cells transfected with fluorescently labeled siRNA?
FAQ ID -793
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How best to handle the light sensitive LiquiChip reagents?
FAQ ID -735
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Do you have information on the use of recombinant DNA and RNA as absolute standards for realtime RT-PCR?
FAQ ID -729
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Is it possible to transfect DNA into insect cell lines such as Drosophila melanogaster S2?
FAQ ID -397
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Is it possible to isolate free genomic DNA using the QIAamp UltraSens Virus Kit?
FAQ ID -358
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Is it possible to store TAGzyme enzymes at°C?
-80
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I accidentally added RNAprotect Tissue Reagent to my cells, and now the cells are difficult to pellet. What can I do?
FAQ ID -364
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What are the calculated molecular weights of the TAGZyme enzymes?
FAQ ID -329
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How complete is the removal of DAPase in the TAGZyme process?
FAQ ID -319
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How long is fluorescence detectable in cells after transfection with fluorescently labeled siRNAs?
FAQ ID -392
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How do I submit a siRNA order by telephone or online?
FAQ ID -399
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How many transfections can I perform with 20 nmol of HPP grade siRNA?
FAQ ID -367
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Which primers can I use for sequencing pQE-expression vector constructs?
FAQ ID -343
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What is the standard assay volume recommended for LiquiChip assays?
FAQ ID -330
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What is the acceptable maximum g force for centrifugation of Xa Removal Resin?
FAQ ID -320
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What is the lowest elution volume that can be used with QIAprecipitator Midi and Maxi Modules?
FAQ ID -307
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What is the composition of Buffer FG3?
FAQ ID -356
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Can I use ethanol instead of isopropanol for DNA precipitation when using HiSpeed Plasmid Kits?
FAQ ID -354
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303 - Can I use my own lysis buffer with the DNeasy Blood & Tissue or QIAamp DNA Mini Kit?
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Can I use a partial MinElute 96 UF plate?
FAQ ID -355
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Can I use the RNeasy Mini Kit or RNeasy 96 Kit with fewer than 100 cells?
FAQ ID -372
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Can I use water for elution from the QIAprecipitator of the HiSpeed Plasmid Kits?
FAQ ID -306
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Are the QIAprecipitator Midi and Maxi Modules of the HiSpeed Plasmid Kits interchangeable?
FAQ ID -308
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Apart from the antibody itself, does the Penta-His Biotin Conjugate solution contain any protein?
FAQ ID -359
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Are QIAGEN monoclonal Anti His Antibodies glycosylated?
FAQ ID -334
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Can I perform in-vitro transcription with the pDrive Cloning Vector from the QIAGEN PCR Cloning Kit?
FAQ ID -332
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Do the QuantiTect SYBR Green Kits contain a stabilization reagent that inhibits the sensitivity of real-time PCR?
FAQ ID -328
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Do I need to anneal, deprotect or desalt my QIAGEN siRNA?
FAQ ID -398
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How are siRNAs introduced into C. elegans?
FAQ ID -396
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Do pQE vectors contain the CAT gene?
FAQ ID -362
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How can I improve the expression of proteins containing hydrophobic regions?
FAQ ID -339
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How can I check if any residual proteins remain on the Ni-NTA Agarose matrix after elution?
FAQ ID -324
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Do freeze-thaw cycles affect the stability of the U-overhang on the pDrive Cloning Vector?
FAQ ID -323
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Can RNAprotect Tissue Reagent be used to store previously isolated RNA?
FAQ ID -337
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Can MinElute 96 UF PCR be used to purify PCR samples which contain mineral oil?
FAQ ID -360
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Does SYBR Green dye present in QuantiTect kits interfere with ethidium bromide staining?
FAQ ID -312
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Do any of the buffers in the HotStarTaq DNA Polymerase Kit contain Triton?
FAQ ID -327
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Do any of the kit components or the product packaging of the QIAamp 96 DNA Blood Kit contain latex?
FAQ ID -318
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What data documentation does QIAGEN's Sequencing Service provide for customers?
FAQ ID -832
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What are the sequences of the FlexiTube siRNAs?
FAQ ID -851
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My BioRobot 9604 came with several Accessory Applications. Is it recommended I run these protocols?
FAQ ID -872
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What is the proper way to turn on or turn off the EZ1 instrument?
FAQ ID -870
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What is the Energy Mix in the EasyXpress Kits?
FAQ ID -846
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What is the concentration of the primers in a reaction using the QuantiTect Primer Assays?
FAQ ID -850
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I would like to sequence my siRNA constructs. Does QIAGEN offer Sequencing Services for cloned siRNA?
FAQ ID -829
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Is it possible to use frozen cell pellets with the FastLane Cell cDNA Kit?
FAQ ID -835
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Is it possible to elute plasmid DNA from the QIAprep Spin Miniprep columns with buffer containing Potassium Phosphate?
FAQ ID -854
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Is it possible to perform immunoprecipitation (IP) directly on protein fractions from the Qproteome Cell Compartment Kit?
FAQ ID -855
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Is it possible to adjust the Lysis Buffer volume used with the Qproteome Bacterial Protein Preparation Kit?
FAQ ID -841
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Is it possible to use QIAGEN's pQE-TriSystem Vectors with the EasyXpress Protein Synthesis Insect Kit?
FAQ ID -876
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Is there a stopping point in the protocol for Suspended Cells using the FastLane Cell cDNA Kit?
FAQ ID -837
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Is the random biotin labeling of proteins expressed with your EasyXpress Biotin Labeling Kit as sensitive as labeling with radioactivity?
FAQ ID -875
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Will biotin detection assays be efficient when doing in vitro translation from E. coli lysates with the EasyXpress Site-Specific Biotin Kit?
FAQ ID -878
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Why would clumps occur following the addition of Buffer P2 when using LyseBlue Reagent in a plasmid preparation?
FAQ ID -862
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Will acetone precipitation recommended in the Qproteome Protocols denature Protein?
FAQ ID -807
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Will site-specific biotin labeling with the EasyXpress Site-Specific Biotin kit alter the structure and function of my protein?
FAQ ID -877
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Will the sequences of the QuantiTect Primer Assays be provided?
FAQ ID -804
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Will the FastLane Cell cDNA Kit work with suspension cells?
FAQ ID -801
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Which resin is used in the QIAexpress Ni-NTA Fast Start Columns?
FAQ ID -836
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What publicly funded Genome Sequencing Projects has QIAGEN's Sequencing Service been involved in?
FAQ ID -815
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What is the typical yield of site-specific labeled protein generated using the EasyXpress Site-Specific Biotin Kit?
FAQ ID -873
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What programs does your Sequencing Service use to ensure accurate base calling, Sequence Quality, and Alignment?
FAQ ID -831
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Where can I find a copy of the report file generated by running 'Maintenance Protocol I' on my BioRobot MDx?
FAQ ID -806
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Which fraction will contain soluble mitochondrial proteins using the Qproteome Cell Compartment Kit?
FAQ ID -839
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Which fraction will contain endosomal, microsomal and lysosomal proteins using the Qproteome Cell Compartment Kit?
FAQ ID -808
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Where can I find QIAGEN products for a specific gene or gene product?
FAQ ID -803
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Can the QuantiTect Primer Assays be used for detection of genomic DNA?
FAQ ID -811
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Do I need to use RNase inhibitors with the RNeasy Kits?
FAQ ID -813
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Do the Buffers CE1 - CE4 of the Qproteome Cell Compartment Kit contain DTT or any reducing agent?
FAQ ID -824
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Can the FastLane Cell cDNA Kit also be used for qualitative end-point PCR?
FAQ ID -834
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Can the FastLane Cell cDNA Kit be used with tissue?
FAQ ID -821
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Do you have a protocol for the isolation of bacteriophage P1 derived constructs with any of your plasmid kits?
FAQ ID -883
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Do you have a protocol for the isolation of BAC DNA using the QIAGEN Plasmid Midi Kit?
FAQ ID -881
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895 - Do you have a protocol for the isolation of endotoxin-free plasmid DNA using the QIAGEN Plasmid Midi Kit?
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Do you have a protocol for isolation of plasmid DNA using the Sigma Centrifuge 4?
-15
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Can Buffer R1 of the R.E.A.L. Prep 96 Plasmid Kit be replaced with Buffer P1?
FAQ ID -823
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Are there specific recommendations for performing RT-PCR on RNA isolated from paraffin-embedded samples?
FAQ ID -828
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Can I stabilize adipose tissue using RNAprotect Tissue Reagent prior to RNA isolation?
FAQ ID -856
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Can I use the PhosphoProtein Purification Kit for the isolation of phosphorylated proteins from yeast?
FAQ ID -838
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Can I use QIAGEN's pQE vectors as templates in the EasyXpress Biotin Labeling Kits?
FAQ ID -874
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Can I use my gene-specific primers with the FastLane Cell cDNA Kit and the QuantiTect Reverse Transcription Kit?
FAQ ID -812
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Can I use LyseBlue with R.E.A.L. Prep 96, QIAwell Ultra, or QIAprep 96 Turbo Miniprep Kits for the BioRobot systems?
FAQ ID -863
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Do you have information on in vivo RNA interference experiments?
FAQ ID -817
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Do you sell the ATP-Dependent Exonuclease of the QIAGEN Large-Construct Kit separately?
FAQ ID -825
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I cannot see the LCD display fonts on the Sigma Centrifuge. How do I adjust the contrast?
FAQ ID -822
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How long does a typical in vitro translation reaction take with your large-scale EasyXpress protein synthesis kits?
FAQ ID -880
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How much protein can be produced with the EasyXpress Protein Synthesis Mega Kit?
FAQ ID -843
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Do you have a protocol for the isolation of very low-copy plasmids from Streptomyces spp.?
FAQ ID -893
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Do you have a protocol for the isolation of plasmid DNA from Citrobacter freundii?
FAQ ID -885
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Do you have a protocol for the isolation of plasmid DNA from Corynebacterium glutamicum?
FAQ ID -886
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Do you have a protocol for the isolation of plasmid DNA from Borrelia spp.?
FAQ ID -884
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Do you have a protocol for the isolation of plasmid DNA from Lactobacillus spp.?
FAQ ID -887
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Do you have a protocol for the isolation of plasmid DNA from yeast?
FAQ ID -853
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Do you have a protocol for the isolation of single-stranded DNA from M13 phage using QIAGEN Plasmid Kits?
FAQ ID -894
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Do you have a protocol for the isolation of plasmid DNA from Staphylococcus spp.?
FAQ ID -890
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Do you have a protocol for the isolation of plasmid DNA from Oligotropha carboxidovorans?
FAQ ID -888
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Do you have a protocol for the isolation of plasmid DNA from Proteus spp.?
FAQ ID -889
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What are your recommendations for cotransfecting several plasmids?
FAQ ID -124
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What are the recommended culture and buffer volumes for a very low-copy plasmid?
FAQ ID -168
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Is mRNA isolation necessary for sensitive RT-PCR?
FAQ ID -111
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When should carrier be used with the QIAamp DNA Mini or the DNeasy Blood & Tissue Kit?
FAQ ID -100
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Which Anti-His Antibody is the most sensitive for my protein of interest?
FAQ ID -172
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Why do I have to add beta-mercaptoethanol (beta-ME) to lysis Buffer RLT of the RNeasy Kits?
FAQ ID -101
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What is the recommended culture medium for the QIAprep System?
FAQ ID -154
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How can I eliminate contaminating protein in my Ni-NTA 6xHis-tag protein purification?
FAQ ID -102
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How can I purify very small amounts of 6xHis-tagged protein using Ni-NTA technology?
FAQ ID -134
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How can I improve ligation efficiency of DNA from a QIAEX II Gel Extraction Kit?
FAQ ID -141
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How can I isolate RNA from 1 gram of plant tissue?
FAQ ID -128
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How can I improve the performance of the HiSpeed QIAprecipitator module?
FAQ ID -144
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What can I use to isolate RNA smaller than 200 nucleotides?
FAQ ID -115
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What epitopes do the Anti-His Conjugates and Anti-His Antibodies recognize?
FAQ ID -170
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What are the maximum culture volumes to use with the QIAGEN Plasmid Midi or Maxi Kit?
FAQ ID -167
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How should RNeasy Kits be stored and how long are they stable?
FAQ ID -103
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Is it possible to cleave the 6xHis-tag from an expressed protein?
FAQ ID -140
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How should QIAGEN Plasmid Purification Kits be stored and for how long?
FAQ ID -192
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What are the features and benefits of the QIAexpress 6xHis Tag System?
FAQ ID -193
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Do you have transfection data for QIAGEN Transfection Reagents?
FAQ ID -158
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Do you have data showing effects of sample size on DNA yield and purity using the DNeasy 96 Blood & Tissue kit?
FAQ ID -129
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Does QIAGEN offer vectors for direct cloning of PCR products?
FAQ ID -146
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How can I avoid variable transfection efficiencies with SuperFect Reagent?
FAQ ID -179
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Can DyeEx be used to clean up labeled DNA other than sequencing templates?
FAQ ID -137
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Are QIAamp DNA isolation kits suitable for apoptosis studies?
FAQ ID -149
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Can I use a QIAfilter Cartridge for purifying large plasmids?
FAQ ID -121
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Can I use Omniscript or Sensiscript RT's at a higher temperature?
FAQ ID -116
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How do you ensure that RNeasy buffers are RNase-free?
FAQ ID -113
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How can I purify DNA from soil, food and sewage samples for PCR?
FAQ ID -118
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How should I store Anti·His and Tag·100 Antibodies?
FAQ ID -194
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How do I prepare an insert for pQE vectors?
FAQ ID -185
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How can I obtain DNA-free RNA using an RNeasy Midi or Maxi Kit?
FAQ ID -143
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What are the compatibilities of different reagents with Ni-NTA matrices?
FAQ ID -49
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How can I prevent running out of M48 Kit reagents before the full number of samples are processed?
FAQ ID -1042
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Do you have a protocol for the isolation of genomic DNA from paraffin-embedded samples on the BioRobot M48?
FAQ ID -1019
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Is the TurboCapture Microplate Holder Type B compatible with the Biomek FX robotic workstation?
FAQ ID -1040
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Is there a limit to the size of proteins that can be purified using the Qproteome Glycoprotein Kits?
FAQ ID -1071
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Is it possible to scale up QuantiTect Reverse Transcription reactions to allow use of larger amounts of RNA?
FAQ ID -1063
浏览
Is it possible to use the PhosphoProtein Purification Kit in batch format?
FAQ ID -1069
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What are the recommended storage conditions for the QuantiTect Reverse Transcription Kit and its components?
FAQ ID -1077
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What is the difference between TurboCapture Microplate Holders Type A and Type B?
FAQ ID -1038
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What blocking reagents do you recommend for chemiluminescent detection using Penta-, Tetra-, and RGS-His antibodies?
FAQ ID -1093
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Is Factor SB of the HotStar HiFidelity Polymerase Kit the same as Factor MP of the QIAGEN Multiplex PCR Kit?
FAQ ID -1049
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How can I transfect siRNA into insect cells such as Drosophila melanogaster S2?
FAQ ID -1046
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How can I tell TurboCapture Microplate Holder Type A from Type B?
FAQ ID -1039
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How much template can I use in the reaction and what is the maximum volume of template that can be used in the QuantiTect Kits?
FAQ ID -1086
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How much dye-labeled siRNA should I use to monitor transfection efficiency when using HiPerfect Transfection Reagent?
FAQ ID -1058
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What is the error rate of HotStar HiFidelity DNA Polymerase?
FAQ ID -1048
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Which QIAGEN kit do you recommend for purifying plasmid DNA suitable for transfection of sensitive cells?
FAQ ID -1092
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Which kit should I use for RNA isolation from Cartilage?
FAQ ID -1026
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Where can I find the serial number on QIAGEN's BioRobots?
FAQ ID -1064
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Which kit can be used for simultaneous purification of DNA and RNA from biopsies?
FAQ ID -1089
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Why is there RNA in the DNA eluate when using the AllPrep DNA Mini Spin Column?
FAQ ID -1044
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Why does my PCR product show up later when comparing the QuantiTect SYBR Green PCR Kits with Roche kits using the same annealing temperature?
FAQ ID -1083
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Will the "classic" RNeasy Mini Kit be discontinued after the launch of the Allprep DNA/RNA Mini Kit?
FAQ ID -1054
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Why does TA/UA cloning work with your proofreading HotStar HiFidelity DNA Polymerase?
FAQ ID -1053
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Why do melting temperatures differ between PCR fragments amplified with QIAGEN's QuantiTect SYBR Green PCR Kits and Roche Kits?
FAQ ID -1084
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What is the range of melting temperatures for the QuantiTect Primer Assay amplicons in the dissociation curve?
FAQ ID -1079
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What is the protein loading capacity per column of the Qproteome Albumin/IgG Depletion Kit?
FAQ ID -1090
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What is your recommendation for cleaning the TissueLyser Bead Dispenser (96-well format)?
FAQ ID -1055
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What is the resin in the Qproteome Murine Albumin and Qproteome Albumin/IgG Depletion Kits?
FAQ ID -1034
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How can I prevent clogging of QIAfilter cartridges?
FAQ ID -1060
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What is the volume of the eluate when using either spin or vacuum procedures with the QIAprep 96 Turbo Miniprep Kit?
FAQ ID -1067
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Do I have to use a ramp time of 2°C/sec on the LightCycler when using Quantitect Primer Assays?
FAQ ID -1065
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Does an activation time of 15 minutes influence the performance of the HotStarTaq Plus DNA Polymerase?
FAQ ID -1050
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Do you have a protocol for the isolation of RNA from bacterial cultures using RNAprotect Bacteria Reagent and QIAzol Lysis Reagent?
FAQ ID -1020
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Do you have a protocol for the isolation of DNA from mouse tails using MagAttract technology on BioRobot M96?
FAQ ID -1007
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Do you have a protocol for the isolation of DNA from forensic samples using the BioRobot M48?
FAQ ID -1017
浏览
Can the USB Port of the BioSprint 96 instrument be used to transfer programs from a PC to the instrument?
FAQ ID -1032
浏览
Are your FlexiTube siRNAs preferentially designed to any particular region of the target gene?
FAQ ID -1091
浏览
Can PCR fragments generated with the EasyXpress Linear Template Kit be cloned into the pIX4.0 vector of the EasyXpress Protein Synthesis Insect Kit?
FAQ ID -1078
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Can the QProteome O-Glycan Glycoprotein Kits be used for blood cells?
FAQ ID -1036
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Can the Reverse Transcriptases of the QuantiTect Reverse Transcription Kit and the QuantiTect Probe RT-PCR Kit be used interchangeably?
FAQ ID -1066
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Can the AllPrep RNA/Protein Kit be used with tissue?
FAQ ID -1095
浏览
Can the Blocking Reagent supplied with the Penta-His HRP Conjugate be used with other antibodies?
FAQ ID -1073
浏览
How can air bubbles in PhosphoProtein Purification Columns be avoided?
FAQ ID -1070
浏览
How can I increase low-copy plasmid DNA yields using QIAfilter Plasmid Kits?
FAQ ID -1062
浏览
How can I ensure complete genomic DNA removal when using the RNase-Free DNase Set?
FAQ ID -1087
浏览
Do you have information on the reproducibility of DNA yields from blood using the M48 BioRobot?
FAQ ID - 1004
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Do you have a protocol for transient transfection of HEK 293 cells in 75 cm3 flasks?
FAQ ID -1014
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Do you have a protocol for transient transfection of cells in 96-well plates using PolyFect Transfection Reagent?
FAQ ID -1013
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Do you have a protocol for transient transfection of HeLa-S3 cells in 60 mm dishes using PolyFect?
FAQ ID -1012
浏览
Do you have a protocol for the isolation of total nucleic acids from cells on the M48 BioRobot?
FAQ ID -1009
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Do you have a protocol for vacuum processing of blood samples with the QIAamp DNA Blood Midi or Maxi Kits?
FAQ ID -1011
浏览
Do you have a protocol for the isolation of total nucleic acids from tissue on the M48 BioRobot?
FAQ ID -1008
浏览
Do you have information on realtime RT-PCR performance of RNA isolated from monocytes on the M48 BioRobot?
FAQ ID -1010
浏览
Do you have information on DNA yields from blood with variable numbers of white blood cells using the M48 BioRobot?
FAQ ID -1006
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Do you have information on PCR template preparation from preserved blood samples on the M48 BioRobot?
FAQ ID - 1003
浏览
Can the PAXgene Blood RNA Tubes undergo freeze/thaw cycles?
FAQ ID -2471
浏览
If I want to use BioMag particles, do I have to buy a QIAGEN magnet too or can I use one that I already have?
FAQ ID -266
浏览
Should I use Omniscript or Sensiscript for reverse transcription of low-copy mRNA?
FAQ ID -297
浏览
What are the main differences between other magnetic beads and the BioMag particles?
FAQ ID -272
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My BioMag is about to expire, is it still any good?
FAQ ID -268
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How magnetically responsive is BioMag?
FAQ ID -265
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If I am using BioMag Streptavidin to capture biotinylated oligos, how can I then remove the oligos?
FAQ ID -279
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Why is an ice-incubation step included during reaction set-up when following the QuantiTect RT-PCR but not the QuantiTect PCR protocol.
FAQ ID -283
浏览
What sequencing primers can I use with the pDrive cloning vector of the QIAGEN PCR Cloning Kit?
FAQ ID -292
浏览
What is the difference between the QIAamp MinElute Virus Spin and the MinElute Virus Vacuum Kit?
FAQ ID -295
浏览
What is the difference between the Fc specific versions of your secondary antibody BioMag particles and the non-specific?
FAQ ID -275
浏览
Can I use my own cycling conditions with the QIAGEN Multiplex PCR Kit?
FAQ ID -287
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Can I select FITC labeled cells with BioMag?
FAQ ID -278
浏览
Can I use BioMag particles with any specific primary antibody?
FAQ ID -273
浏览
Are BioMag particles stable in other solvents?
FAQ ID -267
浏览
Can BioMag be used for positive and negative selection?
FAQ ID -269
浏览
How can I avoid poor immunolocalization morphology with Anti-His Antibodies?
FAQ ID -200
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How can I prevent non-specific binding when using BioMag?
FAQ ID -277
浏览
How do I prevent bubbles from forming in my Ni-NTA agarose column?
FAQ ID -285
浏览
How can I purify mRNA from total RNA using an Oligotex Direct mRNA Kit?
FAQ ID -201
浏览
Is your Nuclease-Free Water fluorescence-free?
FAQ ID -1291
浏览
What quality level does your Nuclease-Free Water have?
FAQ ID -1292
浏览
How should the GelPilot DNA Loading Dye be handled?
FAQ ID -1287
浏览
Does GelPilot DNA Loading Dye contain ethidium bromide?
FAQ ID -1286
浏览
Can phenol/chloroform be placed in the MaXtract tube for extraction at a later time?
FAQ ID -1299
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Are MaXtract tubes siliconized?
FAQ ID -1298
浏览
Should I input the dilution factor of my samples into the LiquiChip Analyser Software dilution factors of samples?
FAQ ID -1211
浏览
What cell lines have been tested with the Qproteome Plasma Membrane Protein Kit?
FAQ ID -1205
浏览
Is a saliva collection device provided in the RNeasy Protect Saliva Mini Kit?
FAQ ID -1213
浏览
Is it possible to increase nucleic acid concentrations when following protocols on the BioRobot EZ1?
FAQ ID -1232
浏览
Is RNAprotect Cell Reagent provided in the RNeasy Protect Cell Kit available separately?
FAQ ID -1217
浏览
Is it possible to use QIAGEN's pQE-Trisystem Vector with the EasyXpress Insect Kit II?
FAQ ID -1225
浏览
What vector do you recommend as template for in-vitro protein expression with the EasyXpress Insect Kit II?
FAQ ID -1224
浏览
What type of promoter should my template have for in-vitro protein expression with the EasyXpress Insect Kit II?
FAQ ID -1223
浏览
What is the maximum number of cells that can be used with the AllPrep RNA/Protein Kit ?
FAQ ID -1208
浏览
Will the Qproteome Plasma Membrane Protein Kit work with starting materials other than adherent mammalian cells?
FAQ ID -1202
浏览
What is the difference between various FlexiTube siRNAs listed for the same target gene, and which one should I choose?
FAQ ID -1206
浏览
What is the protein yield when using the Qproteome Plasma Membrane Protein Kit?
FAQ ID -1201
浏览
What is the size of the BioRobot EZ1?
FAQ ID -1245
浏览
Does anything have to be added to express post-translational modifications (PTMs) with the EasyXpress Insect Kit II?
FAQ ID -1226
浏览
Do you have a protocol for isolating cytoplasmic RNA from animal cells using the RNeasy Mini Kit?
FAQ ID -1207
浏览
Do you have a protocol for purification of miRNA from animal cells using the RNeasy Plus Mini Kit and RNeasy MinElute Cleanup Kit?
FAQ ID -1258
浏览
Can cells stabilized with RNAprotect Cell Reagent be used for cell sorting by flow cytometry (FACS)?
FAQ ID -1216
浏览
Can glycosylated proteins be expressed using the EasyXpress Insect Kit II?
FAQ ID -1227
浏览
Can RNAprotect Saliva Reagent be purchased separately?
FAQ ID -1214
浏览
Can you recommend a Reagent or Kit for stabilizing RNA in cultured cells?
FAQ ID -1215
浏览
Can the Strep-Tactin Magnetic Beads of the Qproteome Plasma Membrane Protein Kit be reused?
FAQ ID -1204
浏览
Can the Qproteome Plasma Membrane Protein Kit be used with tissue samples?
FAQ ID -1200
浏览
Can the RLT lysate of the BioSprint DNA Plant protocol be stored frozen?
FAQ ID -1248
浏览
How can I decontaminate the BioRobot EZ1?
FAQ ID -1231
浏览
How do you ensure that concentrated RNA is purified from saliva using the RNeasy Protect Saliva Mini Kit?
FAQ ID -1212
浏览
How much active protein can be expressed in-vitro using the EasyXpress Insect Kit II, and how much time does it take?
FAQ ID -1222
浏览
How many freeze-thaw cycles can the various reaction mixes in the EasyXpress Insect Kit II tolerate?
FAQ ID -1219
浏览
Do you have a protocol for the isolation of DNA from buccal cells using the BioRobot EZ1?
FAQ ID -1260
浏览
Do you have a protocol for transfection of differentiated macrophage cell lines (THP) with siRNA?
-1
浏览
How are plasma membrane containing vesicles eluted off the ligand in the Qproteome Plasma Membrane Protein procedure?
FAQ ID -1203
浏览
Which fixation method is used in the PAXgene Tissue System?
FAQ ID - 3600
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What is the fixation reagent of PAXgene Tissue FIX?
FAQ ID - 3601
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What is the stabilization reagent of PAXgene Tissue STABILIZER?
FAQ ID - 3602
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What is the maximum tissue size that can be fixed in a PAXgene Tissue FIX Container (50 ml)?
FAQ ID - 3604
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Why are two reagents used in the PAXgene Tissue System?
FAQ ID - 3603
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Which conditions are recommended for the storage of tissues in PAXgene Tissue STABILIZER?
FAQ ID - 3605
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Is it possible to process formalin-fixed and PAXgene Tissue fixed samples together in one run?
FAQ ID - 3606
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Is it necessary to clean a processor normally used for formalin-fixed tissue before using it with PAXgene Tissue fixed tissue?
FAQ ID - 3607
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Is it possible to integrate the PAXgene Tissue STABILIZER into tissue processing?
FAQ ID - 3608
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Are immunohistochemistry (IHC) assays developed for formalin-fixed, paraffin-embedded (FFPE) tissues compatible with PAXgene Tissue fixed, paraffin-embedded (PFPE) tissues?
FAQ ID - 3609
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How well is RNA integrity preserved in PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID - 3611
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Is it possible to use formalin-fixed, paraffin-embedded (FFPE) kits and protocols to isolate biomolecules from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID - 3610
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How well is DNA integrity preserved in PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID - 3612
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Are special kits and protocols required to isolate biomolecules from PAXgene Tissue fixed, cryo-embedded (PFCE) tissue?
FAQ ID - 3614
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What are the yield and integrity of nucleic acids isolated from blocks of PAXgene Tissue fixed, cryo-embedded (PFCE) tissue?
FAQ ID - 3613
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Can proteins be extracted from PAXgene Tissue fixed specimens?
FAQ ID - 3616
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Does the PAXgene Tissue RNA Kit co-purify small RNAs?
FAQ ID - 3615
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Which kits and protocols can be used to isolate nucleic acids from microdissected PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) specimens?
FAQ ID - 3617
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What is the PCR performance of DNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissue compared to DNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?
FAQ ID - 3618
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How is the quality of proteins purified from PAXgene Tissue fixed and stabilized tissue?
FAQ ID - 3619
浏览
Can the RNeasy kits be used to extract RNA from saliva collected using the Oragene collection kit?
FAQ ID - 3623
浏览
How do I analyze codon 61 mutations with the RAS extension kit (cat. no. 971590)?
FAQ ID - 3622
浏览
Are QR codes recognized by QIAsymphony?
FAQ ID - 3625
浏览
Do I need to use a blood collection set with the PAXgene Blood ccfDNA Tube?
FAQ ID - 3626
浏览
What is the reagent in the PAXgene Blood ccfDNA Tube?
FAQ ID - 3632
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Does the insert sequence provided in the specification sheet correspond to the target sequence or to anti-sense sequence?
FAQ ID - 3627
浏览
What is the shelf life of unused PAXgene Blood ccfDNA Tubes?
FAQ ID - 3633
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How should unused PAXgene Blood ccfDNA Tubes be stored?
FAQ ID - 3634
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Are there special considerations for phlebotomy when using the PAXgene Blood ccfDNA Tube (blood collection set, discard tube, positioning of the tube, issues with short draws, etc.)?
FAQ ID - 3635
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Are PAXgene Blood ccfDNA Tubes sterile?
FAQ ID - 3636
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What is the expected ccfDNA yield from plasma generated from 10 ml of blood?
FAQ ID - 3637
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Can I use plasma from underfilled PAXgene Blood ccfDNA Tubes?
FAQ ID - 3638
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How long can whole blood stabilized in PAXgene Blood ccfDNA Tubes be stored at room temperature?
FAQ ID - 3639
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Can whole blood in PAXgene Blood ccfDNA Tubes be stored at temperatures above 25°C?
FAQ ID - 3640
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Can whole blood in PAXgene Blood ccfDNA Tubes be stored at temperatures below 15°C?
FAQ ID - 3641
浏览
Can whole blood be frozen in PAXgene Blood ccfDNA Tubes?
FAQ ID - 3642
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Is double centrifugation required for plasma processing?
FAQ ID - 3643
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How long and at which temperature can plasma generated from whole blood in PAXgene Blood ccfDNA Tubes be stored?
FAQ ID - 3644
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What should I do if cryoprecipitates form while thawing a frozen plasma sample?
FAQ ID - 3646
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Which plasma volumes can I process with the QIAsymphony PAXgene Blood ccfDNA protocols?
FAQ ID - 3648
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How many extractions can I perform with a QIAsymphony PAXgene Blood ccfDNA Kit, using the 2.4 ml or the 4.8 ml protocols?
FAQ ID - 3649
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How long after first use can a reagent cartridge be stored for further use?
FAQ ID - 3650
浏览
For what type of sample material is the QIAsymphony PAXgene Blood ccfDNA Kit intended?
FAQ ID - 3652
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Which tubes are recommended for the QIAsymphony sample rack
FAQ ID - 3653
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Which protocol line should I use to process plasma samples?
FAQ ID - 3654
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Does the QIAsymphony PAXgene Blood ccfDNA Kit also allow eluting ccfDNA in less than 60 µl?
FAQ ID - 3656
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How should eluates produced with the PAXgene Blood ccfDNA System be stored?
FAQ ID - 3657
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Which downstream applications have been tested with ccfDNA purified from whole blood collected and processed with the PAXgene Blood ccfDNA System?
FAQ ID - 3658
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What is the shelf life of the QIAsymphony PAXgene Blood ccfDNA Kit?
FAQ ID - 3651
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Which elution volumes are available with the QIAsymphony PAXgene Blood ccfDNA Kit?
FAQ ID - 3655
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Why is a blood collection set required to collect blood into the PAXgene Blood ccfDNA Tube?
FAQ ID - 3628
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Can reagent from the PAXgene Blood ccfDNA Tube flow back into the patient’s arm?
FAQ ID - 3629
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Can I use a different brand blood collection set with the PAXgene Blood ccfDNA Tube?
FAQ ID - 3630
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What is the blood draw volume of the PAXgene Blood ccfDNA Tube
FAQ ID - 3631
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What is the difference between RT² Profiler PCR Array and RT² Profiler PCR Array PLUS version?
FAQ ID - 3685
浏览
Where can I find the gene list for a specific RT² Profiler PCR Array?
FAQ ID - 3684
浏览
Is it possible to store nucleic acids extracted using the SpeedXtract Virus Kit?
FAQ ID - 3680
浏览
Total RNA from what sample types are compatible with the QIAseq miRNA Library Kit?
FAQ ID - 3660
浏览
Can miRNA sequencing libraries be prepared from total RNA isolated from heparinized plasma samples?
FAQ ID - 3661
浏览
Are any components of the QIAseq miRNA Library Kit interchangeable with components from other QIAGEN kits?
FAQ ID - 3662
浏览
Can I still perform sequencing if my miRNA sequencing library concentrations are too low to obtain a 4 nM library?
FAQ ID - 3666
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What method is used to eliminate adapter dimers from the QIAseq miRNA library?
FAQ ID - 3667
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Does a QIAseq miRNA library include hY4 Y RNA?
FAQ ID - 3670
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What is the sequence of the QIAseq miRNA NGS 3’ Adapter?
FAQ ID - 3671
浏览
Do the covered regions in the BED file include the primers?
FAQ ID - 3689
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What do the GTIN, UDI and REF abbreviations mean on the label of any QIAGEN box?
FAQ ID - 3697
浏览
Do eluates generated with the PAXgene Blood miRNA Kit contain residual genomic DNA?
FAQ ID - 3637
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Do you have a protocol for high-throughput purification of plasmid DNA using the BioRobot Universal System?
FAQ ID -1191
浏览
Is the surface tension of the crystallization supports of the EasyXtal 15-Well Tools similar to that of a siliconized cover slide?
FAQ ID -1107
浏览
Is the pH indicated in the formulation sheet for Screening Suite buffers the final pH?
FAQ ID -1123
浏览
What are the applications the EasyXtal 15-Well Tool can be used for?
FAQ ID -1111
浏览
Is the EasyXtal Tool compatible with liquid-handling systems and visualization robots?
FAQ ID -1118
浏览
Is it possible to collect intact mitochondria before or after the density gradient centrifugation step of the Qproteome Mitochondria Isolation protocol?
FAQ ID -1187
浏览
What cell lines have been tested with the Fastlane Cell cDNA Kit?
FAQ ID -1175
浏览
What does 'QIAGEN Certified Solution' for Protein Crystallization mean?
FAQ ID -1121
浏览
How many protein crystallization conditions are available at QIAGEN?
FAQ ID -1119
浏览
How much detection reagent do you generally recommend for LiquiChip Assays?
FAQ ID -1159
浏览
How many cells can be used with the Qproteome Mitochondria Isolation Kit, and how much mitochondrial protein can be expected?
FAQ ID -1189
浏览
How many drops can be set up on a protein crystallization support?
FAQ ID -1110
浏览
How many LiquiChip beads are recommended for an xMAP experiment?
FAQ ID -1161
浏览
How much solution can I add to an EasyXtal 15-Well Tool reservoir well?
FAQ ID -1114
浏览
In which formats are crystallization condition Screening Suites available?
FAQ ID -1127
浏览
If I cannot find a QuantiTect Primer Assay for my target gene in rat, can I use the assays for the orthologous genes in mouse and human?
FAQ ID -1145
浏览
If a QuantiTect Primer Assay has more than one version number, does this mean that earlier assay versions may not work?
FAQ ID -1136
浏览
How sensitive is the LiquiChip Reader?
FAQ ID -1157
浏览
How much time is needed for a LiquiChip assay point measurement?
FAQ ID -1162
浏览
Why are there various different QuantiTect Primer Assays for my gene (previous versions, transcript variants)?
FAQ ID -1133
浏览
What is the delivery time for QuantiTect Primer Assays?
FAQ ID -1142
浏览
Where can I find the composition tables for solutions used in individual Crystallization Screening Suites?
FAQ ID -1124
浏览
What kind of strategy for initial screening of protein crystallization conditions do you recommend?
FAQ ID -1120
浏览
When searching for a QuantiTect Primer Assay for my gene of interest, why do I only find the assay for human but not for rat?
FAQ ID -1139
浏览
What type of magnet do you recommend for handling the magnetic beads in the Qproteome Plasma Membrane Protein protocol?
FAQ ID -1193
浏览
What starting material can be used with the Qproteome Mitochondria Isolation Kit?
FAQ ID -1184
浏览
Can the EZ1 DNA Investigator Tip Dance protocol cope with any solid matrix remaining in the sample tube?
FAQ ID -1153
浏览
Are EasyXtal 15-Well Tools stackable?
FAQ ID -1116
浏览
Why can I not find the QuantiTect Primer Assay in GeneGlobe that I had previously ordered from QIAGEN?
FAQ ID -1138
浏览
Which QuantiTect Primer Assay should I choose for my gene of interest?
FAQ ID -1135
浏览
Why does the QuantiTect Primer Assay for my gene of interest have only one version number?
FAQ ID -1134
浏览
Which chemical products do you use to prepare screening solutions for protein crystallization?
FAQ ID -1122
浏览
Will proteins eluted in the Qproteome Plasma Membrane Protein protocol be modified, e.g. with the ligand?
FAQ ID -1195
浏览
Will the ligand in the Qproteome Plasma Membrane Protein protocol be specific for plasma membrane exclusively?
FAQ ID -1198
浏览
Will integral, peripheral as well as lipid-anchored plasma membrane proteins be isolated with the Qproteome Plasma Membrane Protein Kit?
FAQ ID -1199
浏览
Why is there no QuantiTect Primer Assay for my gene of interest?
FAQ ID -1140
浏览
What is the EasyXtal 15-Well Tool made of?
FAQ ID -1106
浏览
What is the dynamic range of detection of the LiquiChip Reader?
FAQ ID -1158
浏览
Can the nuclei and cell debris fraction generated in the QProteome Mitochondria isolation procedure be used for further fractionation?
FAQ ID -1185
浏览
Can the EZ1 DNA Forensic Card be used with the EZ1 DNA Investigator Kit?
FAQ ID -1151
浏览
Can the EasyXtal 15-Well Tools be reused?
FAQ ID -1117
浏览
Can the Qproteome Plasma Membrane Protein Kit be used with suspension cells?
FAQ ID -1194
浏览
Can your Tag antibody recognize rat MAPK1 also?
-100
浏览
Can xMAP kits from other Luminex partners be used on the LiquiChip System purchased from QIAGEN?
FAQ ID -1156
浏览
Can the remaining fractions obtained in the Qproteome Plasma Membrane Protein procedure be used in downstream assays?
FAQ ID -1197
浏览
Can the Spin Columns of the Qproteome Albumin/IgG Depletion Kit be reused?
FAQ ID -1173
浏览
Are scoring sheets available for each crystallization condition Screening Suite?
FAQ ID -1125
浏览
Can mitochondria separated with the Qproteome Mitochondria Isolation Kit be used for downstream DNA isolation?
FAQ ID -1188
浏览
Are protein modifications such as glycosylation problematic when using the Qproteome Plasma Membrane Protein Kit?
FAQ ID -1196
浏览
Can acetone be used for precipitation of protein from Buffer RLT lysates generated with RNeasy Kits?
FAQ ID -1164
浏览
Can I increase the annealing temperature recommended for QuantiTect Primer Assays used with the QuantiTect SYBR Green PCR Kits?
FAQ ID -1146
浏览
How are NeXtal deep-well blocks sealed? Can they be resealed?
FAQ ID -1128
浏览
Does the EasyXtal Tool conform to an SBS format?
FAQ ID -1115
浏览
Do you have WGA protocols for starting materials not mentioned in the REPLI-g Handbooks?
FAQ ID -1160
浏览
How easy is it to scoop up a protein crystal with the lip around the crystallization supports of the EasyXtal 15-Well Tools?
FAQ ID -1113
浏览
Do you have a protocol for the purification of 6xHis-tagged proteins using BioSprint?
FAQ ID -1167
浏览
How did you determine that your AllStar Negative Control siRNA binds to RISC?
FAQ ID -1180
浏览
How is the quality of the crystallization condition Screening Suites guaranteed?
FAQ ID -1126
浏览
How do I use EasyXtal Crystallization Tools?
FAQ ID -1105
浏览
How does the sealing efficiency of the crystallization supports of the EasyXtal 15-Well Tool compare to grease?
FAQ ID -1109
浏览
Do you have a kit for protein isolation from mitochondria prepared with your Qproteome Mitochondria Isolation Kit?
FAQ ID -1190
浏览
Do you have a kit for the isolation of RNA from formalin-fixed, paraffin-embedded samples?
FAQ ID -1174
浏览
Do I need an ultracentrifuge for the density gradient centrifugation step in the Qproteome Mitochondria Isolation protocol?
FAQ ID -1186
浏览
Do protein crystals stick to the walls of cavities when using the DropGuard crystallization supports?
FAQ ID -1108
浏览
Do you have a protocol for evaluating pipetting accuracy of the BioRobot EZ1?
FAQ ID -1131
浏览
Do you have a protocol for purification of DNA from stool samples for pathogen detection using BioSprint Instruments?
FAQ ID -1165
浏览
Do you have a protocol for purification of DNA from stool samples using BioSprint Instruments?
FAQ ID -1166
浏览
Do you have a protocol for evaluating temperature accuracy of the BioRobot EZ1?
FAQ ID -1168
浏览
Which competent E. coli cells should I use to amplify the SureSilencing shRNA Plasmids?
FAQ ID -2890
浏览
Which analyses can be performed with the PyroMark Q96 ID software version 1.0?
FAQ ID -2845
浏览
Does the same U1 promoter in the SureSilencing shRNA Plasmids express shRNA in human, mouse, and rat cell lines?
FAQ ID -2887
浏览
How can I use the SureSilencing shRNA Plasmids for stable transfection and knock down in a cell line that is already resistant to both neomycin and puromycin?
FAQ ID -2895
浏览
Can I use my own siRNA design?
FAQ ID -2897
浏览
Can I order the nucleotides from PyroMark Gold Reagents separately?
FAQ ID -2827
浏览
Can I reclone the insert sequences from the SureSilencing shRNA Plasmids into a viral-based vector?
FAQ ID -2896
浏览
Can I directly transfect SureSilencing shRNA Plasmids into my cells?
FAQ ID -2899
浏览
Do any of the SureSilencing shRNA Plasmids contain inducible promoters?
FAQ ID -2888
浏览
Can PyroMark Gold reagents be vortexed?
FAQ ID -2844
浏览
What are the features of the new PyroMark Q96 ID software 2.5?
FAQ ID -2867
浏览
What cell line does QIAGEN use for quality control testing of the SureSilencing shRNA Plasmids?
FAQ ID -2885
浏览
Should I use the SureSilencing shRNA Plasmids with the neomycin-resistance marker, the puromycin-resistance marker, or the GFP reporter gene?
FAQ ID -2882
浏览
What else do I need to complete an experiment using the SureSilencing shRNA Plasmids?
FAQ ID -2889
浏览
What cell lines can I use with the SureSilencing shRNA Plasmids?
FAQ ID -2891
浏览
How long should I wait after transfection before enriching my transfected cells, validating knock down, and looking for phenotypic changes?
FAQ ID -2893
浏览
Is the expression level of miRNA precursors lower compared to that of mature miRNAs?
FAQ ID -2807
浏览
How many times can the CDTs, NDTs, and RDTs be used?
FAQ ID -2865
浏览
What is the PyroMark Q96 Data Converter?
FAQ ID -2868
浏览
What is the pyrosequencing data exchange tool for?
FAQ ID -2864
浏览
What is the composition of Buffer APP?
FAQ ID -2804
浏览
What is the composition of Buffer FRN?
FAQ ID -2802
浏览
What is the composition of Buffer APL?
FAQ ID -2803
浏览
Which analyses can be performed with the PyroMark Q96 MD software?
FAQ ID -2866
浏览
Which PyroMark Gold Q96 Reagent should be used for which instrument and application?
FAQ ID -2836
浏览
What is the SureSilencing shRNA Plasmids guarantee?
FAQ ID -2898
浏览
What primers may I use to sequence the shRNA cassette within the SureSilencing shRNA Plasmids?
FAQ ID -2886
浏览
Does the PyroMark CpG LINE assay target mouse transposons as well?
-1
浏览
How do I find the SureSilencing shRNA Plasmids for my gene of interest?
FAQ ID -2883
浏览
How are the SureSilencing shRNA Plasmids shipped?
FAQ ID -2884
浏览
What is the concentration of SureENTRY Transduction Reagent?
FAQ ID - 3708
浏览
Is it possible to get a Custom RT2 Profiler PCR Array, cat. # 330131, with primer assays targeting transcripts of different species, e.g. assays targeting transcripts from mouse and assays targeting transcripts from human, on one plate?
FAQ ID - 3710
浏览
Is it possible to purchase Buffer VXL which is part of the QIAamp cador Pathogen Mini Kit, (Cat No.: 54104, 54106) separately?
FAQ ID - 3712
浏览
What sample tubes can I use on my QIAsymphony SP system?
FAQ ID - 3713
浏览
How can I decontaminate my QIAsymphony SP / AS system?
FAQ ID - 3714
浏览
Can other QIAGEN buffers from non-UCP kits (e.g. AW1 instead of AUW1) be used with the QIAamp UCP DNA Micro Kit (cat. no. 56204)?
FAQ ID - 3717
浏览
How is the Pyromark Q48 cartridge to be cleaned?
FAQ ID - 3718
浏览
Can the disc and absorber strips for the Pyromark Q48 be reused?
FAQ ID - 3720
浏览
What is the dead volume of the cartridges in PyroMark Q48?
FAQ ID - 3719
浏览
How much DNA is needed in the PyroMark Q48?
FAQ ID - 3721
浏览
Can I use PyroMark Q48 for diagnostics?
FAQ ID - 3722
浏览
Can I transfer my assays from PyroMark Q24/Q96 to PyroMark Q48 Autoprep?
FAQ ID - 3723
浏览
Can I analyze my Next Generation Sequencing (NGS) samples on the QIAxcel for Quality control?
FAQ ID - 3724
浏览
Is an additional proteinase K digest possible with DNeasy PowerSoil Kit and DNeasy PowerLyzer Kit?
FAQ ID - 3725
浏览
Can I buy the 1 ml Bead Plates from the DNeasy UltraClean 96 Microbial Kit (384) separately?
FAQ ID - 3726
浏览
Does QIAGEN offer an anti-GFP gapmer control?
FAQ ID - 3739
浏览
Why do I observe a CT shift for the Internal Control in some samples in comparison to the CT value for the Internal Control of the dilution series of the Male Control DNA M1?
FAQ ID - 3745
浏览
How long can I store the dilutions of the Male Control DNA M1 standards at 2–8°C?
FAQ ID - 3744
浏览
Do I need to buy special kits for the QIAcube Connect?
FAQ ID - 141485
浏览
Can I program my own protocols for the QIAcube Connect?
FAQ ID - 141486
浏览
Can the QIAcube Connect rotor and/or buckets be removed for cleaning?
FAQ ID - 141491
浏览
Can purification columns from other suppliers be processed on the QIAcube Connect?
FAQ ID - 141487
浏览
How can I get software updates for the QIAcube Connect?
FAQ ID - 141494
浏览
Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube Connect?
FAQ ID - 141496
浏览
Do I need to discard partially used QIAcube Connect tip racks?
FAQ ID - 141499
浏览
How can I decontaminate the QIAcube Connect system?
FAQ ID - 141500
浏览
Can the QIAcube Connect heater/shaker be used independently from protocol runs?
FAQ ID - 141502
浏览
How can I load new protocols onto the QIAcube Connect?
FAQ ID - 141501
浏览
What dedicated QIAcube Connect kits are available?
FAQ ID - 141504
浏览
Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube Connect?
FAQ ID - 1414507
浏览
Where can I find the ordering information for QIAcube Connect accessories such as shaker rack plugs, rack labeling strips, reagent bottle racks, 30 ml reagent bottles, rotor-adapter holder, rotor adapters, sample tubes CB, sample tubes RB and spin-column adapter rings?
FAQ ID - 141505
浏览
Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube Connect?
FAQ ID - 141506
浏览
Which QIAcube Connect standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet?
FAQ ID - 141508
浏览
How often should the o-ring for the pipettor tip adapter be changed on the QIAcube Connect?
FAQ ID - 141509
浏览
Can the CB and RB sample tubes be used interchangeably?
FAQ ID - 141510
浏览
Which network settings are supported by the QIAcube Connect and Q-Base?
FAQ ID - 141517
浏览
Why can I not find the Q-Base device in the device list during the first installation?
FAQ ID - 141516
浏览
Why can’t I see my QIAcube Connect instrument in the QIAcube Connect App?
FAQ ID - 1414518
浏览
Why is the QIAcube Connect App reacting so slowly?
FAQ ID - 141519
浏览
Why are not all applications/kits/protocol files visible in the run setup (QIAcube Connect App)?
FAQ ID - 1414520
浏览
Why does the connection test fail?
FAQ ID - 141522
浏览
How can I accurately determine the amount of input material for the EpiTect Hi-C Kit?
FAQ ID -
143065
浏览
What sample source do you support?
FAQ ID -
143066
浏览
How long does it take to complete the EpiTect Hi-C protocol?
FAQ ID -
143068
浏览
How many reactions are in a kit?
FAQ ID -
143067
浏览
Can I use cells that have been previously crosslinked and frozen (e.g., for a ChIP or ChIP-seq experiment) as input material for the EpiTect Hi-C protocol?
FAQ ID -
143069
浏览
Can I substitute the endonuclease used in the Hi-C Digestion step with another endonuclease?
FAQ ID -
143070
浏览
Are there stopping points in the Hi-C workflow?
FAQ ID -
143071
浏览
With which platform can I perform my sequencing?
FAQ ID -
143072
浏览
With which read length should the EpiTect Hi-C NGS libraries be sequenced
FAQ ID -
143073
浏览
How do I know if my Hi-C sample is a good candidate for deep sequencing?
FAQ ID -
143074
浏览
What kind of analysis does the EpiTect Hi-C Data Analysis Portal perform?
FAQ ID -
143076
浏览
Can I use my own pipeline to analyze results from my EpiTect Hi-C experiments?
FAQ ID -
143078
浏览
How critical is the time of the temperature change from 60°C to 80°C?
FAQ ID -
143768
浏览
Is it possible to use Lyse&Spin baskets?
FAQ ID -
143762
浏览
Why is DTT used only for semen samples?
FAQ ID -
143761
浏览
Can the Repli-g Advanced DNA Single Cell Kit also be used for bacterial cells?
FAQ ID -
143079
浏览
Can the lysis volume be reduced for higher sensitivity?
FAQ ID -
143763
浏览
What if only one heater shaker is available?
FAQ ID -
143764
浏览
Why is there no male DNA obtained from sexual assault samples?
FAQ ID -
143767
浏览
If 400 µl are used as lysis volume, how do I concentrate the lysate? (Otherwise the DNA would be too diluted.)
FAQ ID -
143766
浏览
Why is the QIAamp Viral RNA Mini Kit (50) currently unavailable?
FAQ ID -147395
浏览
What RNAs has QIAseq FastSelect been designed to remove?
FAQ ID -147910
浏览
How does QIAseq FastSelect work?
FAQ ID -147907
浏览
Is QIAseq FastSelect truly as easy as combining a reagent with RNA and ramping down the temperature?
FAQ ID -147909
浏览
Will QIAseq FastSelect work in other species?
FAQ ID -147911
浏览
What library prep kits has QIAseq FastSelect been tested with?
FAQ ID -147912
浏览
I am using an RNA library prep kit that is not listed in the QIAseq FastSelect handbook. Will QIAseq FastSelect work with my kit?
FAQ ID -147913
浏览
What RNA range has the QIAseq FastSelect been tested with?
FAQ ID -147914
浏览
Can QIAseq FastSelect Plant or QIAseq FastSelect Yeast be combined with FastSelect 5S/16S/23S?
FAQ ID -147915
浏览
Is it possible to test the efficiency of FastSelect rRNA removal by using a Bioanalyzer, etc.?
FAQ ID -147916
浏览
Is plasmid DNA purified with QIAGEN Plasmid Purification Kits suitable for in vitro transcription?
FAQ ID -1
浏览
How should I quantify RNA isolated with RNeasy Kits?
FAQ ID -32
浏览
When is chloramphenicol amplification of plasmids performed?
FAQ ID -3
浏览
What are the expected DNA yields from different tissues using the QIAamp DNA Mini Kit?
FAQ ID -45
浏览
What are the most commonly used protease inhibitors?
FAQ ID -53
浏览
How should I propagate pQE expression plasmids?
FAQ ID -58
浏览
How can I increase the amount of soluble recombinant protein in E. coli expression?
FAQ ID -64
浏览
How can I remove imidazole from a protein sample?
FAQ ID -91
浏览
Why does my isolated RNA have a low OD 260/280 ratio?
FAQ ID -97
浏览
How can I avoid little or no RNA yields when using an RNeasy Kit?
FAQ ID -28
浏览
Do I need to use an RNase inhibitor in my RT reaction?
FAQ ID -119
浏览
Can I use QIAprep Miniprep kits for low-copy plasmids and cosmids?
FAQ ID -127
浏览
Can I use the QIAquick PCR Purification Kit for restriction enzyme cleanup?
FAQ ID -130
浏览
How does imidazole affect my quantitation of protein?
FAQ ID -132
浏览
Can a QIAquick Gel Extraction Kit be used to obtain RNA from a formaldehyde gel?
FAQ ID -133
浏览
What is the difference between disruption and homogenization in the RNeasy System?
FAQ ID -139
浏览
What is the size of genomic DNA that is obtained with QIAGEN Genomic-tips?
FAQ ID -142
浏览
Should I use Ni-NTA Agarose in column or batch format for purification of 6xHis-tagged proteins?
FAQ ID -147
浏览
What is the genotype of the EZ Competent Cells?
FAQ ID -157
浏览
What are the restriction sites of the pDrive Vector in the QIAGEN PCR Cloning Kit?
FAQ ID -160
浏览
What are the molecular weights of proteins in the 6xHis Protein Ladder?
FAQ ID -169
浏览
How can I optimize the transfection of oligos and large plasmids using PolyFect Transfection Reagent?
FAQ ID -176
浏览
How can I improve recoveries when using the QIAquick Kits?
FAQ ID -180
浏览
How can I improve transfection efficiency using Effectene Transfection Reagent?
FAQ ID -181
浏览
What is the principle behind Effectene Transfection Reagent?
FAQ ID -184
浏览
What is the composition of Buffer EB?
FAQ ID -199
浏览
Is it possible to modify the transduction protocol for Cignal Lenti Reporter Assays to save some pipetting steps?
FAQ ID - 3709
浏览
Can I store the QIAseq Beads of the QIAseq Targeted DNA Panel at -20°C?
FAQ ID - 3711
浏览
What is the difference between the QIAamp UCP DNA Micro Kit (cat. no. 56204) and RNeasy UCP Kits (cat. no. 73934) and non-UCP QIAGEN purification kits?
FAQ ID - 3715
浏览
Can I use carrier RNA with the RNeasy UCP Micro Kit (cat. no. 73934)?
FAQ ID - 3716
浏览
How can I check for purity of RNA isolated using RNeasy Kits?
FAQ ID -1023
浏览
What is the recipe for 1x PBS solution?
FAQ ID -1030
浏览
Where can I find a protocol for cleanup of already purified plasmid DNA?
FAQ ID -1031
浏览
How do I perform an Acetone Precipitation for concentrating and desalting protein samples?
FAQ ID -1035
浏览
What is the difference between Buffers RLT and RLT Plus?
FAQ ID -1043
浏览
What is the largest PCR amplicon that can be amplified with the HotStar HiFidelity Polymerase Kit?
FAQ ID -1047
浏览
Are the CoralLoad dyes in the HotStarTaq Plus PCR Buffer visible when loading small amounts of PCR product onto a gel?
FAQ ID -1051
浏览
Do I have to use CoralLoad Gel loading dye when using your HotStarTaq Plus DNA Polymerase?
FAQ ID -1052
浏览
How can I increase DNA concentration using QIAprecipitators of the HiSpeed Plasmid Kits?
FAQ ID -1061
浏览
What should I use as a standard for absolute quantification in real-time PCR?
FAQ ID -1085
浏览
What does transcript variant mean when searching for specific QuantiTect Primer Assays?
FAQ ID -1137
浏览
What is a QuantiTect Primer Assay?
FAQ ID -1141
浏览
Do QuantiTect Primer Assays contain SYBR Green dye?
FAQ ID -1143
浏览
What is the smallest and the largest protein that you tested for expression with the EasyXpress Insect Kit II?
FAQ ID -1218
浏览
What are the new features of the EasyXpress Insect Kit II compared to the original Protein Synthesis Insect kit?
FAQ ID -1220
浏览
What are your recommendations for PCR template preparation for use with the EasyXpress Insect Kit II?
FAQ ID -1221
浏览
Do you have a protocol for transfection of suspension cell lines (Jurkat and K562) with siRNA?
FAQ ID -1250
浏览
Do you have a protocol for transfection of macrophage cell lines (J774.A1 and RAW 264.7) with siRNA?
FAQ ID -1251
浏览
What is the pH value of QIAGEN's Nuclease-Free Water?
FAQ ID -1290
浏览
Why is there DNA in the no-template control reaction when using the standard REPLI-g procedure, but not when using the UltraFast procedure?
FAQ ID -1327
浏览
Will the REPLI-g UltraFast Mini Kit reaction always yield 10 µg DNA?
FAQ ID -1328
浏览
Can the bacterial pellet in the QuickLyse Miniprep procedure be resuspended by pipetting up and down or shortening the vortexing time?
FAQ ID -1352
浏览
How can I increase the DNA yield from low-copy plasmids using the QuickLyse Miniprep Kit?
FAQ ID -1354
浏览
What is the composition of elution buffer QLE in the QuickLyse Miniprep Kit?
FAQ ID -1356
浏览
How do I resuspend my FlexiPlate siRNAs?
FAQ ID -1360
浏览
Are there any specific cleaning solutions recommended for the QIAcube?
FAQ ID -1416
浏览
Why is a 2-step (and not a 3-step) cycling protocol recommended for QuantiFast SYBR Green PCR Kits?
FAQ ID -1450
浏览
Can tissue homogenized in Buffer RLT Plus instead of Buffer RLT be used with the AllPrep DNA/RNA/Protein Mini Kit?
FAQ ID -1577
浏览
What can be done if the protein pellet does not solubilize completely using the AllPrep DNA/RNA/Protein Mini Kit?
FAQ ID -1580
浏览
What is the binding capacity of the Ni-NTA Superflow Cartridges?
FAQ ID -1603
浏览
How comparable is CoralLoad gel loading dye contained in various QIAGEN PCR Kits to Sigma Red?
FAQ ID -1644
浏览
Do I need to resuspend FlexiTube siRNAs in siRNA Resuspension buffer?
FAQ ID -1659
浏览
Are FlexiTube siRNA and FlexiTube GeneSolution also available for rat siRNAs?
FAQ ID -1664
浏览
What is the composition of Buffer PE?
FAQ ID -1691
浏览
What is the upper length of PCR products that can be amplified with TopTaq DNA Polymerase and Master Mix Kits?
FAQ ID -1738
浏览
What is the minimum sample volume required for the QIAxcel System and QIAxcel Advanced?
FAQ ID -1852
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What are the dimensions and weight of the QIAsymphony SP System?
FAQ ID -1901
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Are there any specific cleaning solutions recommended for the QIAsymphony SP?
FAQ ID -1913
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How much sample can be used with the RNeasy Plus 96 Kit?
FAQ ID -1992
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What is the composition of Buffer P2?
FAQ ID -203
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A white precipitate has formed in my 10x RT buffer. Is it still ok to use?
FAQ ID -216
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For how long are BioMag Particles stable?
FAQ ID -262
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I used to buy BioMag particles from a company by another name? Are your BioMag particles the same thing?
FAQ ID -263
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Can I clean up my DNase treated RNA samples using RNeasy columns?
FAQ ID -286
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How can one determine the optimal annealing temperature for a specific PCR assay?
FAQ ID -288
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What is the composition of the QIAGEN Multiplex PCR Buffer?
FAQ ID -289
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Can I use HEPES buffer instead of phosphate in my Ni-NTA column?
FAQ ID -291
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Can I use a centrifuge instead of vacuum when using the QIAquick 96 PCR Purification Kit?
FAQ ID -293
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What are the ideal and the largest PCR amplicon sizes when using the EpiTect MSP Kit?
FAQ ID -2004
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What is elution Buffer ATE of the QIAsymphony DNA Investigator Kit?
FAQ ID -2029
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How can a C-terminal 6xHis-tagged QIAgene be expressed?
FAQ ID -2041
浏览
Why is there more than one QIAgene for my gene of interest?
FAQ ID -2044
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Is a passive internal reference dye, like ROX, required on the Rotor-Gene Q to obtain reproducible results?
FAQ ID -2079
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Does the Rotor-Gene Q software have the capacity to export data?
FAQ ID -2089
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Why is a 2-step (and not a 3-step) cycling protocol recommended for Rotor-Gene SYBR Green Kits?
FAQ ID -2122
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Does carrier RNA have to be added using the QIAsymphony Virus Blood 200 Protocol?
FAQ ID -2163
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Can I use the RNeasy Microarray Tissue Mini Kit for all tissue types?
FAQ ID -2190
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How is the RNeasy Microarray Tissue Mini Kit different from the classic RNeasy Mini Kit?
FAQ ID -2192
浏览
Why is EvaGreen instead of SYBR Green used as fluorescent dye in the Type-it HRM PCR Kit?
FAQ ID -2196
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Why do I need normalization using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2198
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What is the difference between probability and typicality in the Rotor-Gene ScreenClust HRM Software?
FAQ ID -2203
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What buffer conditions give the best resolution for agarose gel electrophoresis?
FAQ ID -2256
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How much DNA should be loaded per well of an agarose gel?
FAQ ID -2259
浏览
Why do I have wavy DNA bands on my agarose gel?
FAQ ID -2260
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What is the "seed sequence" of a miRNA?
FAQ ID -2262
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Are you able to supply a Certificate of Analysis for the KRAS and EGFR PCR kits?
FAQ ID -2301
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Which Rotor-Gene Q rotor should be used with the EGFR RGQ PCR kit?
FAQ ID -2315
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How is the RNeasy Plus Universal Kit different from the RNeasy Plus Kit?
FAQ ID -2340
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How does the gDNA eliminator solution of the RNeasy Plus Universal Kit work?
FAQ ID -2341
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How long can I store my purified DNA/RNA isolated with the AllPrep DNA/RNA FFPE kit?
FAQ ID -2350
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Do you have any information or guidelines regarding the choice of reference genes for real-time PCR?
FAQ ID -2371
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Can I buy QIAquick and MinElute columns separately?
FAQ ID -2460
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What is the PAXgene 96 filter plate?
FAQ ID -2486
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Is RNA from red blood cells also isolated when RNA is extracted from a PAXgene Bone Marrow RNA Tube?
FAQ ID -2509
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Is the 260/230 nm absorbance ratio specified for RNA derived from the PAXgene Bone Marrow RNA System?
FAQ ID -2514
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How long is the fixation time?
FAQ ID -2519
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What is the nature of the heterologous Internal Amplification Control and Positive Control in the mericon Assay kits?
FAQ ID -2543
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Are there any accessories required for sample disruption with the TissueLyser LT?
FAQ ID -2551
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Why do the Internal Control templates for extraction (Internal Control DNA or RNA [High conc.]) have a 10x higher concentration than the IC templates provided with the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2603
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During data analysis, how should the threshold be set in the Yellow channel on the Rotor-Gene Q cycler to analyze the Internal Control from the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit
FAQ ID -2607
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What is the SBTengine® software?
FAQ ID -2628
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How do I create a workspace that is free of DNA contamination, prior to carrying out a qPCR experiment?
FAQ ID -2654
浏览
What is the recommended solution in which to store RNA samples that will be used as templates for cDNA synthesis?
FAQ ID -2659
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How can I avoid or remove genomic DNA contamination from the total RNA preparation?
FAQ ID -2662
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What are the differences between one-step and two-step RT--PCR?
FAQ ID -2666
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What is qPCR?
FAQ ID -2668
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What are the common primer and probe chemistries utilized for qPCR assays?
FAQ ID -2669
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What is the purpose of the ROX and fluorescein dyes, also known as passive reference dyes, in qPCR master mixes?
FAQ ID -2671
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What negative controls are typically included in qPCR and/or qRT-PCR experiments?
FAQ ID -2672
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What positive controls are typically included in qPCR and/or qRT-PCR experiments?
FAQ ID -2673
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What are the guidelines for choosing a housekeeping gene for normalizing qPCR results?
FAQ ID -2674
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Why is 18S ribosomal RNA (rRNA) used as a housekeeping gene to normalize sample-to-sample, systematic variation in qPCR assays?
FAQ ID -2675
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How does HotStart PCR help minimize nonspecific amplification events?
FAQ ID -2676
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What is a dissociation curve, and why is it important to run a dissociation curve, following qPCR using SYBR Green chemistry?
FAQ ID -2678
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What is the delta Rn value?
FAQ ID -2681
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What is the threshold cycle or Ct value?
FAQ ID -2682
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Why are my qPCR Ct values too low (< 12) in my qRT-PCR Assay?
FAQ ID -2684
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Why are my qPCR Ct values too high (> 35 or not detectable) in my qRT-PCR assay?
FAQ ID -2685
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Why is my no template control (NTC) real-time Ct value < 35 cycles in my qPCR Assay?
FAQ ID -2686
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Why do my qPCR amplification curves or plots decrease in fluorescence intensity after the saturation phase?
FAQ ID -2689
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Why do I see multiple high-intensity peaks in my qPCR dissociation curve at temperatures less than 70 ºC?
FAQ ID -2690
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What is the standard curve method for qPCR assay data analysis? How is the standard curve method for qPCR assay data analysis performed?
FAQ ID -2691
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What is the difference between Absolute Quantification and Relative Quantification in qPCR, using the standard curve approach?
FAQ ID -2692
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What is the comparative or ??Ct method for qPCR assay data analysis? How is the comparative or ??Ct method for qPCR assay data analysis performed?
FAQ ID -2693
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How do I determine the amplification efficiency of my qPCR assay?
FAQ ID -2694
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How do I determine the linear dynamic range of my qPCR or qRT-PCR assay?
FAQ ID -2696
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What is the best approach for determining where to set the CT threshold when you have >15 samples? Is it best to go through all of them, looking for a range of best fit, and then just choose one value that fits all of them?
FAQ ID -2705
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What do I need to complete a RT² qPCR Primer Assay?
FAQ ID -2707
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How can I prevent the evaporation of reaction volume from the wells in the miScript miRNA PCR array?
FAQ ID -2729
浏览
What is the minimum amount of genomic DNA required for analysis using EpiTect Methyl qPCR Arrays?
FAQ ID -2739
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How do you design your EpiTect ChIP qPCR Assay primers? Do they work with SB GR?
FAQ ID -2749
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How can I identify the binding sites of my transcription factor using ChIP?
FAQ ID -2753
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At what point can I stop and freeze my samples when using ChIP?
FAQ ID -2754
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What is RNA interference (RNAi)?
FAQ ID -2755
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What are the key factors for success in an RNAi experiment?
FAQ ID -2758
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What criteria should one use in choosing between siRNA versus shRNA for their studies?
FAQ ID -2771
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How does stable transfection of a shRNA-encoding plasmid work?
FAQ ID -2779
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What are the SureSilencing shRNA Plasmids?
FAQ ID -2784
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What is the composition of Buffer S3?
FAQ ID -2788
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What is the composition of Buffer ETR?
FAQ ID -2789
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What is the composition of Buffer BB?
FAQ ID -2790
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What is the composition of Buffer RLT plus?
FAQ ID -2794
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What is the composition of Buffer RLC?
FAQ ID -2795
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What is the composition of Buffer RPE?
FAQ ID -2797
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What is the composition of Buffer RWT?
FAQ ID -2798
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What is the composition of gDNA Eliminator Solution?
FAQ ID -2799
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What is the composition of Buffer RDD?
FAQ ID -2800
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What is the composition of Buffer PKD?
FAQ ID -2801
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What is the composition of Buffer ALO?
FAQ ID -2805
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What is the composition of RBC Lysis Solution?
FAQ ID -2808
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What is the composition of Cell Lysis Solution?
FAQ ID -2809
浏览
What is the composition of Protein Precipitation Solution?
FAQ ID -2810
浏览
What is the composition of Cell Suspension Solution?
FAQ ID -2811
浏览
What is the composition and concentration of Glycogen Solution in Gentra Puregene Kits?
FAQ ID -2812
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What is the composition of DNA Hydration Solution in Gentra Puregene Kits?
FAQ ID -2813
浏览
What is the meaning of the abbreviations NDT and CDT and what are the differences?
FAQ ID -2841
浏览
What are the changes of PyroMark Assay Design Software Version 2.0 compared to 1.0?
FAQ ID -2849
浏览
Can I use fluorescence microscopy to assess the transfection efficiency of the SureSilencing shRNA Plasmids with GFP in my model cell line of interest?
FAQ ID -2894
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Do tissue samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction?
FAQ ID -2926
浏览
What is the composition of Buffer G2?
FAQ ID -2943
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What is the composition of Buffer B1?
FAQ ID -2944
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What is the composition of Buffer B2?
FAQ ID -2945
浏览
How much DNA does a human cell contain?
FAQ ID -2948
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How much RNA does a bacterial cell contain?
FAQ ID -2949
浏览
How much DNA and RNA can be expected from human blood cells?
FAQ ID -2950
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What is the cellular composition of human blood?
FAQ ID -2951
浏览
How can I desalt and concentrate a protein fraction by trichloroacetic acid (TCA) precipitation?
FAQ ID -2954
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Is there a risk of crosstalk from the FAM channel to the VIC channel if the residual DNA target is present in very high copy numbers?
FAQ ID -2980
浏览
Why is the final heat denaturation step so important with the QIAsymphony PAXgene Blood RNA protocol?
FAQ ID -2987
浏览
What is the sequence of the miScript Universal Primer?
FAQ ID -2994
浏览
How can I keep my centrifuge tubes endotoxin-free?
FAQ ID -301
浏览
Can Buffers N3 and P3 be used interchangeably?
FAQ ID -310
浏览
Are QIAprep and QIAquick Spin columns interchangeable?
FAQ ID -311
浏览
Can I store agarose gel slices containing DNA for gel extraction at a later point?
FAQ ID -313
浏览
How can QIAGEN Protease and Proteinase K be inactivated?
FAQ ID -315
浏览
Does the 5x OneStep RT-PCR Buffer contain BSA?
FAQ ID -326
浏览
What is the origin of replication and the plasmid copy number of the pQE vectors?
FAQ ID -338
浏览
Is QIAGEN carrier RNA sold separately?
FAQ ID -351
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What is the white insoluble precipitate in my resuspended plasmid DNA pellet?
FAQ ID -352
浏览
What is the composition of PBS?
FAQ ID -361
浏览
How can I express toxic protein in E. coli?
FAQ ID -373
浏览
How can I improve DNA yields from very tough tissues using the DNeasy Blood & Tissue Kit or the QIAamp DNA Mini Kit?
FAQ ID -374
浏览
What are the dissociation constants for the Anti-His Antibodies?
FAQ ID -385
浏览
What is the average molecular weight of a siRNA, and how do I convert uM to ug values?
FAQ ID -388
浏览
Can siRNA silence bacterial genes?
FAQ ID -395
浏览
How should I reconstitute the lyophilized RNase-Free DNase Set with the RNeasy 96 kit?
FAQ ID -3003
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Is it possible to use a standard processor — the kind used routinely for formalin-fixed samples — for dehydration and paraffin infiltration of PAXgene Tissue treated samples?
FAQ ID -3032
浏览
Can I use Tungsten Carbide beads for RNA extraction protocols using QIAGEN Kits?
FAQ ID -3053
浏览
Where can I find the ordering information for QIAcube accessories such as Shaker Rack plugs, Rack labeling strips, Reagent bottle rack, 30mL Reagent bottles, Rotor Adapter holder, Rotor adapters, Sample tubes CB, Sample tubes RB and Spin column adapter rings?
FAQ ID -3054
浏览
What is the part number for O-rings for QIAsymphony SP and AS instruments? How often should the O-rings be replaced?
FAQ ID -3055
浏览
What is the thermal output for a BioRobot MDx and BioRobot Universal?
FAQ ID -3056
浏览
What is the thermal output for a QIAsymphony SP and AS instrument?
FAQ ID -3057
浏览
How precise is the QIAgility?
FAQ ID -3063
浏览
What are the advantages and disadvantages of turning on the tip “re-use” feature on the QIAgility?
FAQ ID -3064
浏览
How long does it take to set up a 96-well plate PCR reaction on the QIAgility?
FAQ ID -3066
浏览
Can I use a bar code scanner or reader on the QIAgility?
FAQ ID -3067
浏览
How can I find the run parameters for a BioSprint protocol that I am running?
FAQ ID -3069
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Can BioSprint 96 be connected to LIMS?
FAQ ID -3070
浏览
What is the expected level of endotoxins in plasmid DNA purified with QIAprep spin miniprep kit?
FAQ ID -3081
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What is the expected level of endotoxins in plasmid DNA purified with QIAGEN Plasmid kits, QIAfilter Plasmid kits and HiSpeed Plasmid purification kits?
FAQ ID -3082
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What is the concentration of the Gentra RNase A that is used with the Autopure and Puregene protocols?
FAQ ID -3083
浏览
What is the concentration of the different Proteinase K solutions sold by QIAGEN?
FAQ ID -3084
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Where can I access Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit?
FAQ ID -3085
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What tubes can be used with TissueLyser Adapter Set 2 x 96 (cat. no. 69984)?
FAQ ID -3086
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How to upload a Flexiplate on QIAGEN Webpage?
FAQ ID -3103
浏览
Can we do modification XXXXX for custom siRNA that is not on the web page?
FAQ ID -3107
浏览
What is the stability of siRNA lyophilized and resuspended?
FAQ ID -3109
浏览
Do you offer FlexiTube or FlexiPlate siRNAs for rat?
FAQ ID -3114
浏览
Is it possible to extract RNA from frozen blood?
FAQ ID -3119
浏览
Is it possible to extract RNA from RNAprotect-stabilized-blood?
FAQ ID -3120
浏览
What's the pulsed-field gel electrophoresis (PFGE) conditions of the gel picture showing on your website? http://www.qiagen.com/Search/MagAttract-HMW-DNA-Kit#productdetails
-48
浏览
Can Dithiothreitol (DTT) be used instead of BME in Buffer RLT Plus of RNeasy Plus Mini Kit?
FAQ ID -3125
浏览
Why does my purified DNA/RNA sometimes have a 260/280 or 260/230 ratio of more than 2? Does that mean the purity is poor?
FAQ ID -3132
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How do I remove the RNA contamination from purified DNA?
FAQ ID -3133
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What is the final concentration of Protease upon reconstitution in the QIAamp DNA Blood Mini?
FAQ ID -3140
浏览
What is the Spike-in-Control in the miRNeasy Serum/Plasma Kit ?
FAQ ID -3169
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What is the difference between the 1 nmol FlexiTube and FlexiTube Gene Solution siRNAs?
FAQ ID -3174
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What could be a reason for cloudy precipitate in PCR reaction with STR Plus chemistry after cycling protocol is completed?
FAQ ID -3175
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What is needed for performing delta delta Ct relative quantitation on the Rotor-Gene Q?
FAQ ID -3178
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Is REST software Windows 7 compatible?
FAQ ID -3182
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Can the Rotor-Gene Q perform gradient PCR?
FAQ ID -3183
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What is the expected amplicon size of the Microbial DNA qPCR Assays?
FAQ ID — 3396
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What is the difference between Positive PCR Control (PPC) and Microbial DNA Positive Control?
FAQ ID — 3397
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What is LLOQ?
FAQ ID — 3401
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What is the difference between LLOQ and LOD?
FAQ ID — 3402
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What species are detected by the Pan Bacteria 1 and Pan Bacteria 3 Assays?
FAQ ID - 3427
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What is the composition of Buffer AC in the QIAamp UltraSens Virus Kit?
FAQ ID - 3429
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Can the miScript II RT Kit be used to quantify plant miRNAs?
FAQ ID - 3431
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What is the dissociation curve temperature of PCR products generated with the miScript Plant qPCR system?
FAQ ID - 3444
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Can specific types of white blood cells be enriched from a PAXgene Blood RNA Tube prior to the RNA isolation?
FAQ ID - 3467
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What is the proper disposal procedure for waste from the sample preparation?
FAQ ID - 3470
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What is the expected yield from 8.5 ml of blood?
FAQ ID - 3496
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What does the resuspension buffer, Buffer BG4, in the PAXgene Blood DNA kit contain?
FAQ ID - 3501
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How should blood samples drawn into PAXgene Blood DNA Tubes be transported?
FAQ ID - 3507
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How long can samples in PAXgene Blood DNA Tubes be stored at room temperature?
FAQ ID - 3508
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What is the expected DNA yield from 2.5 ml of blood when using the QIAsymphony, QIAcube, and salting-out precipitation method?
FAQ ID - 3510
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Which downstream applications have been tested with DNA purified from samples collected in PAXgene Blood DNA Tubes IVD?
FAQ ID - 3512
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What does PAXgene Tissue STABILIZER contain?
FAQ ID - 3513
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What is the minimum number of cells that can be efficiently processed with the QIAamp DNA Mini and QIAamp Blood Mini kits and what is the expected yield?
FAQ ID - 3516
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Is the quality and size of DNA extracted with the DNeasy Blood & Tissue kit good enough to generate DNA-libraries for next generation sequencing?
FAQ ID - 3517
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What is the minimum number of cells or minimum amount of tissue that can be efficiently processed with the RNeasy mini kit?
FAQ ID - 3519
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Can I use the RNeasy Fibrous Tissue Mini Kit (cat. no. 74704) to extract RNA from fibrous plant material?
FAQ ID - 3521
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Will the Reverse transcription control on the RT2 profiler PCR array work on any cDNA library?
FAQ ID - 3534
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Microscopic/visual confirmation of successfully sorted cells can be used to optimize sorting conditions. Can stained cells be used?
FAQ ID - 3559
浏览
Is the miRNA reverse transcription control (miRTC) used in conjunction with the miScript Single Cell qPCR Kit?
FAQ ID - 3573
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Is clot detection possible on the QIAsymphony?
FAQ - ID 3588
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How can I determine if a tube is suitable for clot detection on QIAsymphony?
FAQ ID - 3590
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How should I thaw frozen plasma samples?
FAQ ID - 3645
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How do Unique Molecular Indexes (UMIs) improve quantification?
FAQ ID - 3669
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Can REPLI-g WGA be used to amplify cDNA if the cDNA is dsDNA (double-stranded DNA)?
FAQ ID - 3686
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I would like to stop during the library preparation procedure. When can I do that?
FAQ ID - 3687
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Which regions are covered by the GR DNAseq V2 Panels (#181900)?
FAQ ID - 3688
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When preparing plasma with the PAXgene Blood ccfDNA tubes, cat. # 768115, do you recommend that the first centrifugation step should be done with the brakes on or off?
FAQ ID - 3690
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How do I insert and replace an absorber strip into the PyroMark Q48 Autoprep chamber?
FAQ ID - 3691
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Are peak heights of Q48 comparable to other PyroMark instruments?
FAQ ID - 3692
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How much space does the PyroMark Q48 Autoprep instrument need?
FAQ ID - 3693
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Can unused wells in a pyrosequencing discs be used in the next run?
FAQ ID - 3694
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Where can I find the certificate of calibration for the Rotor-Disc OTV Kit (catalog number 981400)?
FAQ ID - 3695
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What is the minimum guaranteed shelf-life of the PAXgene Blood ccfDNA Tubes (100) (catalog no. 768115)?
FAQ ID - 3696
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What methods are used during normalization of RNAseq data in the secondary GeneGlobe Data Analysis Center?
FAQ ID - 3698
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How do I prepare Buffer MP?
FAQ ID - 3620
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Are the QIAshredder columns included in various QIAGEN kits the same?
FAQ ID - 3624
浏览
What is the composition of buffer QBT?
FAQ ID -411
浏览
What is the composition of buffer QC?
FAQ ID -412
浏览
What is the composition of buffer QF?
FAQ ID -413
浏览
What is the composition of buffer STE?
FAQ ID -415
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What is the composition of buffer TE?
FAQ ID -416
浏览
What is the composition of buffer FWB2?
FAQ ID -417
浏览
What is the composition of buffer P3?
FAQ ID -418
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What is the size, charge and isoelectric point of the DHFR protein in the pQE vectors?
FAQ ID -470
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Why is carrier RNA used during the isolation of gDNA from microdissected samples with the QIAamp DNA Micro Kit?
FAQ ID -473
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Why are the centrifugation speeds for the QIAamp DNA Mini kit at 6000 x g? Can I spin at full speed?
FAQ ID -474
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Are HEPES, MOPS and PIPES buffers compatible with QIAGEN's PhosphoProtein Purification kit?
FAQ ID -482
浏览
Can Ni-NTA resins be used to purify protein with an internal His-tag?
FAQ ID -496
浏览
How do I calculate the percentage of silencing with real-time RT-PCR for siRNA?
FAQ ID -498
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Do you have a protocol for the removal of endotoxins from already purified plasmid DNA?
FAQ ID -500
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Do you have stability data for genomic DNA isolated with the QIAamp DNA Blood Mini Kit?
FAQ ID -518
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What is the composition of the siRNA resuspension & annealing buffer?
FAQ ID -522
浏览
Can Taq DNA Polymerase use RNA as a template, and generate false positives in "no-RT" controls?
FAQ ID -523
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What is Tissue-Tek O.C.T., and what is it used for?
FAQ ID -530
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Why do replicates in real-time PCR have different plateau heights?
FAQ ID -539
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How can I avoid primer-dimer formation during PCR amplification?
FAQ ID -544
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How can I tell if I have primer-dimers in my PCR reaction?
FAQ ID -552
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How should I store cDNA produced using Omniscript Reverse Transcriptase?
FAQ ID -561
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Is it possible to isolate DNA from bone marrow with the QIAamp DNA Blood Kits?
FAQ ID -563
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What is the composition of Buffer ER? Is it available separately?
FAQ ID -571
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What can I do when the DNA pellet prepared with QIAGEN Plasmid Kits has been overdried?
FAQ ID -572
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Do I have to remove the oil from my PCR reaction before using the QIAquick or MinElute PCR Purification Kit?
FAQ ID -575
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Are the columns of the QIAquick PCR Purification-, Gel Extraction-, and Nucleotide Removal Kit interchangeable?
FAQ ID -577
浏览
What size is the smallest protein that can be synthesized with the EasyXpress Protein Synthesis Kit?
FAQ ID -600
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Why do I sometimes get light blue colonies when using the QIAGEN PCR Cloning Kit?
FAQ ID -603
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What do you recommend for the cleanup of genomic DNA (gDNA)?
FAQ ID -618
浏览
How can I improve my RNA yield from liver sample when using RNeasy Kits?
FAQ ID -623
浏览
Why do you recommend using Triton X for the purification of 6xHis-tagged protein?
-100
浏览
What 96-well plates do you recommend for use with the LiquiChip Workstation?
FAQ ID -630
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What is a QIAshredder? Is it sufficient for complete disruption and homogenization of my tissue sample?
FAQ ID -631
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Why does the QIAamp DNA Mini Tissue Protocol require both ATL and AL buffer, while the Blood Protocol only uses AL?
FAQ ID -633
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Will the QIAquick PCR Purification Kit remove sufficient SYBR Green from real-time PCR reactions to allow sequencing?
FAQ ID -637
浏览
What is the shelf-life of my QIAGEN Kit?
FAQ ID -651
浏览
What are the differences between MDA and DOP/PEP methods of Whole Genome Amplification?
FAQ ID -665
浏览
Can I purchase Phi29 DNA polymerase only?
FAQ ID -708
浏览
What is the average DNA concentration obtained using the DirectPrep 96 Miniprep Kit?
FAQ ID -721
浏览
I accidentally stored Buffer RDD of the RNase-Free DNase Set at°C. Will it still function?
-20
浏览
I ran my RNA out on an agarose gel and can see lots of bands similar to a ladder. Why?
FAQ ID -745
浏览
How much DNA is obtained in the average PCR reaction?
FAQ ID -750
浏览
Do you have information about the cleanup of single-stranded DNA (ssDNA) with QIAquick columns?
FAQ ID -759
浏览
How can I get a certificate for quality assurance (ISO 13485 and ISO 9001) from QIAGEN?
FAQ ID -762
浏览
What is the difference between Ni-NTA Agarose and Ni-NTA Superflow?
FAQ ID -764
浏览
What is the composition of Buffer N3?
FAQ ID -767
浏览
Are the protocols from the BioSprint software compatible with the KingFisher software and vice versa?
FAQ ID -770
浏览
How should fluorescent labeled probes be stored?
FAQ ID -784
浏览
Is it possible to use the QuantiTect Reverse Transcription Kit with bacterial RNA?
FAQ ID -785
浏览
Are Buffer PB of the QIAquick PCR Purification Kit and Buffer QG of the QIAquick Gel Extraction Kit interchangeable?
FAQ ID -786
浏览
How can I get specific information and a quote for my individual Sequencing Service needs?
FAQ ID -792
浏览
Can the QIAprep Spin Miniprep Kit be used for isolating plasmid DNA from mammalian cells?
FAQ ID -795
浏览
How can I separate PCR fragments that are small and very close in size on an agarose gel?
FAQ ID -800
浏览
Can I reuse the Ni-NTA Agarose and Ni-NTA Superflow resins?
FAQ ID -802
浏览
What Disinfectant Solution do you recommend for the BioRobot MDx?
FAQ ID -809
浏览
Is the E. coli Host Strain M15[pREP4] resistant to Zeocin?
FAQ ID -842
浏览
What annealing temperature should be used with the QuantiTect Primer Assays?
FAQ ID -849
浏览
Why is it recommended to add 1 mM IPTG for optimal protein yields using the EasyXpress Protein Synthesis Kits?
FAQ ID -860
浏览
Which QIAGEN plasmid preparation kits will contain LyseBlue Reagent?
FAQ ID -865
浏览
Do you have an in vitro translation kit allowing the incorporation of seleno-methionine for X-Ray crystallography based protein analysis?
FAQ ID -879
浏览
Do you have a protocol for the isolation of genomic DNA from frozen clotted blood?
FAQ ID -900. Test
浏览
Do you have protocols for the isolation of genomic DNA from tissue using QIAGEN-tips 2500 (Mega) and 10000 (Giga)?
FAQ ID -907
浏览
Do you have a protocol for the isolation of genomic DNA from bone?
FAQ ID -908
浏览
What do you suggest to prevent degradation of RNA isolated from tissue with high amounts of RNases using RNeasy?
FAQ ID -942
浏览
Do you have a protocol for Cyanine 570, Cyanine 670, or biotin labeling of cDNA?
FAQ ID -959
浏览
Do you have a protocol for reverse transfection of adherent cells with siRNA in 384-well plates?
FAQ ID -971
浏览
Do you have a protocol for the purification of PCR products using the BioSprint System?
FAQ ID -984
浏览
Do you have a protocol for transient transfection of Hela-S3 cells using PolyFect Transfection Reagent?
FAQ ID -997
浏览
How can I analyze proteins from exosomes and other extracellular vesicles (EVs) obtained with the exoEasy Maxi Kit by SDS-PAGE?
FAQ ID - 3707
浏览
How can I increase expression of my 6xHis-tagged protein in E. coli?
FAQ ID -63
浏览
Can miRNA and other RNAs smaller than 200 nucleotides be isolated with the AllPrep DNA/mRNA Nano Kit?
148723
浏览
What is the minimum amount of starting material that can be used with the DNA/mRNA Nano Kit?
148724
浏览
What is the composition of buffer OW2?
FAQ ID -419
浏览
What is the composition of buffer OBB?
FAQ ID -421
浏览
Do I need to buy special kits for the QIAcube Connect MDx?
148725
浏览
What applications are offered on the QIAcube Connect MDx instrument?
148726
浏览
Can I program my own protocols for the QIAcube Connect MDx?
148727
浏览
Can purification columns from other suppliers be processed on the QIAcube Connect MDx?
148728
浏览
What are the dimensions and weight of the QIAcube Connect MDx?
148729
浏览
Can the QIAcube rotor adapters be autoclaved?
148730
浏览
Can the QIAcube rotor and/or buckets be removed for cleaning?
148731
浏览
Can the QIAcube be connected to a laboratory information management system?
148732
浏览
Are run reports and/or log files available on the QIAcube?
148733
浏览
How can I get software updates for the QIAcube Connect MDx?
148734
浏览
Can QIAcube Connect MDx protocol runs be interrupted, and subsequently be continued to completion?
148735
浏览
Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube?
148736
浏览
Do I need to discard partially used QIAcube tip racks?
148738
浏览
How can I decontaminate the QIAcube Connect MDx system?
148739
浏览
How can I load new protocols onto the QIAcube Connect MDx?
148740
浏览
Can the QIAcube Connect MDx heater/shaker be used independently from protocol runs?
148741
浏览
Can the QIAcube Connect MDx centrifuge be used independently from protocol runs?
148742
浏览
What dedicated QIAcube Kits are available?
148743
浏览
Can I ask QIAGEN to upgrade my current QIAcube Connect Life Science instrument to make it an MDx instrument?
148744
浏览
Can I run life science and custom protocols on a QIAcube Connect MDx?
148745
浏览
What are the heating/cooling rates in the QIAquant instruments?
153919
浏览
Is a passive internal reference dye, like ROX, required on the QIAquant to obtain reproducible results?
153920
浏览
What sample volume is suitable for use in the QIAquant?
153921
浏览
What types of reaction vessels are required for use in the QIAquant?
153922
浏览
Is the QIAquant equipped with a temperature gradient function?
153923
浏览
Are the 96- and 384-sample blocks interchangeable?
153924
浏览
Is it possible to use the touch down PCR option on QIAquant with two acquisition points in the same channel?
153925
浏览
How many molecules can be detected in the system?
153926
浏览
What applications can the QIAquant be used for?
153927
浏览
Can I upgrade my QIAquant 96 2plex instrument to QIAquant 96 5plex or modify the set of color channels?
153928
浏览
Does QIAGEN offers arrays and assays configured specifically for QIAquant instrument?
153929
浏览
Is the software of the QIAquant compatible with 21CFR part11?
153930
浏览
Is it possible to import a standard curve from a previous run?
153947
浏览
Can data be analyzed while the run is proceeding? Can data be analyzed from a previous experiment while another experiment is running?
153948
浏览
Can I use QIAquant 96 Software to analyze experiments carried out on QIAquant 384?
153950
浏览
Can I operate QIAquant 96 instrument using touchscreen and computer simultaneously?
153951
浏览
Is it possible to establish an internet connection with QIAquant instrument?
153952
浏览
Can I connect more than one QIAquant instrument to the same computer?
153953
浏览
What is the algorithm behind data normalization in the QIAquant Software?
153954
浏览
Is it possible to edit the sample sheet after the run in the QIAquant Software?
153955
浏览
What are the LIMS capacity of the software?
153949
浏览
Does the QIAquant Software have the capacity to export data? Which data formats can be used for the export?
153946
浏览
I received a kit containing the MinElute columns; however, they were left out for a while and not stored at 2–8°C upon receipt. Can I still use them?
FAQ ID - 3560
浏览
Is the DNA extracted with the QIAamp DNA FFPE Advanced Kits suitable for downstream applications that require more intact DNA such as long range PCR (>1 kb) and long read DNA sequencing?
153891
浏览
What are recommended stopping points in the procedure of the QIAamp DNA FFPE Advanced Kits?
153892
浏览
Can samples be lysed overnight with Proteinase K in the QIAamp DNA FFPE Advanced procedure?
153893
浏览
Can the second Proteinase K lysis step in the QIAamp DNA FFPE Advanced procedure be carried out at lower temperatures than 65°C?
153894
浏览
Can the 2nd Prot K step be omitted?
153896
浏览
What size of DNA can be expected?
153897
浏览
What is the difference of buffer FTB versus Buffer ATL?
153895
浏览
Is it possible to change voltage set-up from 110V to 230V on the QIAcuity instruments?
3761
浏览
How can I upgrade the software on the instrument (CSW) and the QIAcuity Software Suite?
3762
浏览
Can I see error codes on the instrument touchscreen?
3763
浏览
How are results stored within the QIAcuity Software Suite?
3764
浏览
What is the scope of a regular maintenance of the QIAcuity?
3765
浏览
Can the QIAcuity One 2plex be upgrade to the QIAcuity One 5plex?
3766
浏览
Can I use an empty nanoplate without seal to perform a dry run?
3767
浏览
Does an incorrect handling of a plate might affect the results?
3768
浏览
What is the impact of not applying the latest VPF? Can I reanalyze previously obtained results after installing the latest VPF?
3769
浏览
Can I see on the QIAcuity Software Suite report file if the VPF (Volume Precision Factor) has been used or not?
3770
浏览
Is it necessary to reanalyze a plate with VPF (Volume Precision Factor) that was already processed using a QIAcuity instrument that was purchased in 2020?
3771
浏览
How can I get the latest VPF (Volume Precision Factor)?
3772
浏览
Where can I find assays specifically designed for QIAcuity?
3773
浏览
Can I prepare a dPCR reaction directly in QIAcuity Nanoplate?
3774
浏览
Can I use the dPCR master mix in a qPCR cycler for optimization purposes?
3775
浏览
What is the purpose of the reference channel in QIAcuity?
3776
浏览
How to prepare DNA prior to dPCR?
3778
浏览
Can I use a custom master mix instead of a QIAGEN master mix?
3777
浏览
Which sample preparation method is recommended for QIAcuity dPCR?
3779
浏览
What are the storage conditions and expiry date of QIAcuity consumables?
3780
浏览
What is the input volume for QIAcuity Nanoplate well?
3782
浏览
Can I use the QIAcuity Nanoplate in more than one runs?
3781
浏览
What is the VPF (Volume Precision Factor)?
3784
浏览
When and how often do I need a new VPF (Volume Precision Factor)?
3785
浏览
Is a standard curve needed in dPCR?
3786
浏览
Which database versions are used for CRISPR-Q Custom PCR Assay and CRISPR-Q Sanger Primers design?
3788
浏览
Is there anything to consider when processing cultured cells on coated cultivation dishes? Are additional purification steps required to eliminate or reduce coating reagents such as Poly-L-Lysine?
3791
浏览
Is there something to consider when working with transduced cells treated with Polybrene?
3790
浏览
For sequencing application, does the lysate require purification?
3792
浏览
Are the CRISPR-Q PCR Assays and the CRISPR-Q Sanger Primers on GeneGlobe validated?
3793
浏览
Are the PCR products generated with CRISPR-Q Custom PCR Assays only applicable to analysis of editing efficiency by Sanger sequencing?
3794
浏览
Can the new CRISPR PCR Assays be used for dPCR on the QIAcuity? Do you have data or a protocol?
3795
浏览
What is the minimum cell number needed for cell lysis?
3789
浏览
For the Sanger analysis tool, the customer needs to upload two .abi1 files of forward and reverse sequences? Is there any additional info that needs to be provided?
3796
浏览
What is the sequence of the QIAseq miRNA NGS 5’ Adapter?
FAQ ID - 3672
浏览
What are recommended stopping points in the procedure of the EZ1&2 DNA FFPE Kits?
3753
浏览
Are there other options for paraffin removal?
3754
浏览
Can samples be lysed overnight with Proteinase K in the EZ1&2 DNA FFPE procedure?
3755
浏览
Can the second Proteinase K lysis step in the EZ1&2 DNA FFPE procedure be carried out at lower temperatures than 65°C?
3756
浏览
What is the difference between Buffer FTB and Buffer ATL?
3757
浏览
Can the 2nd Proteinase K step be omitted?
3758
浏览
What size of DNA can be expected?
3759
浏览
Is the DNA extracted with the EZ1&2 DNA FFPE Kits suitable for downstream applications that require more intact DNA such as long-range PCR (>1 kb) and long-read DNA sequencing?
3760
浏览
Do you recommend 1-step or 2-step real-time RT-PCR for gene expression analysis?
FAQ ID -1056
浏览
3323 - Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube?
FAQ ID - 3323
浏览
Does salt concentration in the food matter when using the DNeasy mericon Food Kit?
FAQ ID - 3346
浏览
Why does the DNeasy mericon Food Kit use a QIAquick column and Buffer PB rather than a DNeasy column and Buffer AL, which might be expected since the kit isolates genomic DNA?
FAQ ID - 3347
浏览
When would I use the mericon DNA Bacteria Kit vs the mericon DNA Bacteria Plus Kit?
FAQ ID - 3348
浏览
Why is my 260/280 ratio low after using the DNeasy mericon Food Kit?
FAQ ID - 3349
浏览
CAn I use milk or other liquids with the DNeasy mericon Food kit? What volume would I use?
FAQ ID - 3350
浏览
What is the composition of QIAzol? What is the color of this reagent?
FAQ ID - 3355
浏览
Is it possible to stop the DNeasy tissue protocol and store the tissue lysates after digesting in buffer ATL and Proteinase K?
FAQ ID - 3362
浏览
I cannot analyze the data from your Investigator HID kits with my GeneMapper software. What can I do?
FAQ ID - 3363
浏览
What is the QIAxcel QX RNA Size Marker 200-6000nt?
FAQ ID - 3365
浏览
What is the nature of the gel in the QIAxcel RNA QC kit v2.0? Is it denaturing?
FAQ ID - 3366
浏览
Do you sell CoralLoad dye separately?
FAQ ID - 3368
浏览
Are QIAcube reagent bottles (cat. no. 990393) autoclavable? What type of plastic are they made of?
FAQ ID - 3369
浏览
How long can samples in PAXgene Blood DNA Tubes (IVD) be stored at 2–8°C?
FAQ ID - 3379
浏览
Which downstream applications have been tested with DNA purified from samples collected in PAXgene Blood DNA Tubes (IVD)?
FAQ ID - 3384
浏览
What is the composition of elution buffers used in QIAsymphony DNA Investigator kits?
FAQ ID - 3387
浏览
Can I buy the RNeasy Mini columns, RNeasy MinElute columns, RNeasy Midi columns or the RNeasy Maxi columns separately?
FAQ ID - 3388
浏览
Can I buy the gDNA eliminator plates supplied in your RNeasy Plus 96 kit separately?
FAQ ID - 3389
浏览
Can I buy the gDNA eliminator columns supplied in your RNeasy Plus kits separately?
FAQ ID - 3390
浏览
Why does AllPrep DNA/RNA/Protein Mini kit use buffer RLT, but Allprep DNA/RNA Mini and Allprep DNA/RNA Micro kits use buffer RLT Plus? Are these buffers interchangeable?
FAQ ID - 3391
浏览
Do you have data to show lowest dropout rate with Repli-G WTA Single Cells kit?
FAQ ID - 3392
浏览
What is the RNase A concentration and composition of Buffer P1?
FAQ ID -198
浏览
Are protocols also available for other instruments (e.g., Hamilton Star and Tecan Fluent)?
3797
浏览
Where can I download the protocol for the KingFisher Flex?
3798
浏览
Who can help in case of issues with the third party instrument?
3799
浏览
Is the virus inactivated during the procedure?
3800
浏览
Can I use sample volumes other than 300 µl?
3801
浏览
Who can help if it is not clear if issues are caused by the third party instrument or by the chemistry?
3802
浏览
I would like to use the kit on another third party instrument. Who should I contact?
3803
浏览
How long does a saliva collection take?
3804
浏览
What can I do to stimulate saliva flow?
3805
浏览
Can I stop saliva collection before the fill line is reached?
3806
浏览
What can I do if I don't see the fill line anymore due to saliva bubbles?
3807
浏览
What happens if I accidentally unscrew the funnel before saliva collection is completed?
3808
浏览
May I continue with the saliva collection if I accidentally unscrewed the funnel prematurely?
3809
浏览
What is in the additive in the PAXgene Saliva Collector?
3810
浏览
What is the shelf life of unused PAXgene Saliva Collectors?
3811
浏览
How should unused PAXgene Saliva Collector be stored?
3812
浏览
What should I avoid before sample collection?
3813
浏览
How does the stabilizing reagent in the PAXgene Saliva Collector stabilize saliva?
3814
浏览
How long can saliva in PAXgene Saliva Collector tubes be stored at room temperature?
3815
浏览
Can saliva in PAXgene Saliva Collector tubes be stored at temperatures higher than 25°C?
3816
浏览
Can saliva in PAXgene Saliva Collector tubes be stored frozen at –20 or –80°C?
3817
浏览
Is RNA preserved in saliva collected in PAXgene Saliva Collector?
3818
浏览
Are pathogens inactivated in human saliva samples collected with the PAXgene Saliva Collector?
3819
浏览
What should I do if stabilized saliva samples are very heterogenous?
3820
浏览
What is the expected DNA purity from saliva collected and stabilized with the PAXgene Saliva Collector?
3823
浏览
Is it possible to process volumes of saliva from the PAXgene Saliva Collector other than the 400 or 1000 µl from the standard protocols for the QIAsymphony DNA Midi Kit using the QIAsymphony SP instrument?
3824
浏览
Is it possible to purify DNA from the whole sample of a PAXgene Saliva Collector in one preparation?
3826
浏览
Which downstream applications have been tested with DNA purified from saliva collected into PAXgene Saliva Collector?
3827
浏览
Which downstream applications have been tested with SARS-CoV-2-derived RNA purified from saliva collected into PAXgene Saliva Collector?
3828
浏览
Is a restriction digest necessary for DNA isolated from PAXgene Saliva samples before performing a dPCR run using the QIAcuity?
3829
浏览
Is it possible to place the PAXgene Saliva Collector tube directly on the QIAsymphony SP instrument sample rack?
3825
浏览
What is the expected DNA yield per milliliter saliva collected and stabilized with the PAXgene Saliva Collector?
3822
浏览
Which QIAGEN DNA extraction kits are compatible with the PAXgene Saliva Collector?
3821
浏览
What are common sized libraries observed on a TapeStation, Bioanalyzer, or similar instruments?
FAQ ID - 3837
浏览
Are the QIAseq miRNA NGS 5’ Adapter and QIAseq miRNA NGS RT Initiator compatible with the QIAseq miRNA UDI kits?
FAQ ID - 3836
浏览
What is the sequence of the UDI 5' Adapter?
FAQ ID - 3835
浏览
Can you perform qPCR analysis of miRNAs from libraries prepared with the QIAseq miRNA Library Kit?
FAQ ID - 3674
浏览
What can be used to validate results obtained with the QIAseq miRNA Library Kit?
FAQ ID - 3675
浏览
What is the maximum number of available sample indexes?
FAQ ID - 3665
浏览
What are recommended stopping points during the QIAseq miRNA Library Kit procedure?
FAQ ID - 3663
浏览
Should total RNA or small enriched RNA be used as the starting material for the QIAseq miRNA Library Kit?
FAQ ID - 3659
浏览
What is the recommended read length for libraries prepared using the QIAseq miRNA Library Kit?
FAQ ID - 3668
浏览
What is the acceptable number of reads per sample per miRNA sequencing run?
FAQ ID - 3673
浏览
Do you have a protocol for the isolation of genomic DNA from whole blood for use in MRC-Holland MLPA® assays?
FAQ ID -1169
浏览
Is training of lab personnel included with the purchase of a BioRobot EZ1?
FAQ ID -1239
浏览
Do the foils sealing the EZ1 Reagent Cartridges have to be manually removed prior to loading the robot?
FAQ ID -1240
浏览
How can we get our BioRobot EZ1 upgraded when new protocols are launched?
FAQ ID -1241
浏览
Where can I find information about the worktable setup on the BioRobot EZ1?
FAQ ID -1243
浏览
What disposables are required for nucleic acid isolations on the BioRobot EZ1?
FAQ ID -1246
浏览
How should I prepare buffy coat samples for use on the BioRobot EZ1?
FAQ ID -1249
浏览
Do you have a protocol for the isolation of DNA from buffy coat using the BioRobot EZ1?
FAQ ID -985
浏览
What has to be done to an RNA sample before loading it onto an Agilent Bioanalyzer?
FAQ ID -528
浏览
Do you have a protocol for the isolation of total nucleic acids from animal and human tissues on the BioRobot EZ1?
FAQ ID -975
浏览
Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the BioRobot EZ1?
FAQ ID -976
浏览
Do you have a protocol for the isolation of DNA from fungi using the BioRobot EZ1?
FAQ ID -992
浏览
Are the new EZ1 RNA Kits (cat. no. 958034; 959034; 956034) compatible with the previous EZ1 RNA Card (cat. no. 9015590) or EZ1 RNA Universal Tissue Card (cat. no 9016384)?
FAQ ID -1025
浏览
What are the advantages of EnzScript M-MLV Reverse Transcriptase RNase H-(P7600) versus wild type M-MLV Reverse Transcriptase (P7040)?
FAQ ID - 3838
浏览
What types of priming are compatible with EnzScript M-MLV Reverse Transcriptase RNase-H-?
FAQ ID - 3839
浏览
What is the optimal reaction temperature for EnzScript M-MLV Reverse Transcriptase RNase H-?
FAQ ID - 3840
浏览
What PCR enzymes can be used following the first-strand synthesis?
FAQ ID - 3841
浏览
What is the suggested protocol for generating long, full-length cDNA transcripts (>5 kb)?
FAQ ID - 3842
浏览
What region of LINE gets targeted by the assay for PyroMark Q96 MD or PyroMark Q24?
2857
浏览
Does the PyroMark LINE assay target all LINE sequences?
2860
浏览
What is the amplicon length of the PyroMark CpG LINE assay?
2856
浏览
How do I perform the PyroMark Q24 Advanced upgrade?
FAQ ID -3163
浏览
Is it possible to use PyroMark Q24 Advanced reagents on PyroMark Q24 system?
FAQ ID -3160
浏览
What are the differences between new PyroMark Q24 Advanced reagents chemistry and PyroMark Q24 Gold reagents?
FAQ ID -3165
浏览
How accurate and reliable is PyroMark Q24 in mutation analysis?
FAQ ID -2094
浏览
We recently changed the OS from Windows XP to Windows 7. When re-installing software Pyromark Q24 2.0.6, it fails to analyze the results. Any suggestions?
FAQ ID - 3621
浏览
What is the use of the PyroMark Q24 Validation Oligo?
FAQ ID -2855
浏览
How does the built-in QC control for complete bisulfite conversion of DNA work on PyroMark Q24?
FAQ ID -2095
浏览
How long does a single run on the PyroMark Q24 take?
FAQ ID -2096
浏览
3843-How-do-I-prevent-a-drifting-baseline-in-my-pyrosequencing-pyrogram
FAQ ID - 3843
浏览
When do I have to change the pulse settings/methods in a pyrosequencing run setup?
FAQ ID -2942
浏览
What is a PyroMark instrument method or instrument code?
FAQ ID -2941
浏览
Can the PyroMark Q96 CpG LINE assay be used with an ID system?
2861
浏览
How many times can the cartridges for PyroMark Q24 or PyroMark Q96 ID instruments be reused?
FAQ ID -2863
浏览
How do I prevent a drifting baseline in my pyrosequencing pyrogram? If this method cannot be selected automatically in the application software, you can download the method/code file from the instrument webpage.
FAQ ID -2878
浏览
Can unused wells in a pyrosequencing plate be used in the next run?
FAQ ID -2872
浏览
What is the reason for signals ceasing in the middle of a pyrosequencing run?
FAQ ID -2875
浏览
Is the CpG software included in the PyroMark instruments to study methylation status?
FAQ ID -2842
浏览
What is the reason for split peaks appearing in between dispensations on my pyrosequencing pyrogram?
FAQ ID -2881
浏览
Where can I find explanations to the warning given by the PyroMark software after run data analysis?
FAQ ID -2874
浏览
Which heating block is recommended for the pyrosequencing annealing step?
FAQ ID -2838
浏览
How many times can vacuum troughs be reused with the PyroMark Vacuum Preparation Stations?
FAQ ID -2848
浏览
What kind of shaker should be used for the pyrosequencing binding step?
FAQ ID -2837
浏览
What is the reason for a high substrate peak in the pyrosequencing pyrogram?
FAQ ID -2879
浏览
How many CpG sites are analyzed by the PyroMark CpG LINE assay?
2858
浏览
Is there a user manual available for the PyroMark Assay design software?
FAQ ID -2851
浏览
Which operating system is compatible with PyroMark IdentiFire Software?
FAQ ID - 3340
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How do I set up a PyroMark CpG Assay?
FAQ ID -2814
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What is the purpose of the unmethylated and unconverted control DNA of the EpiTect PCR Control DNA Set?
FAQ ID -2007
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Will the primer sequence for the PyroMark CpG Assay be provided?
FAQ ID -2823
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Does QIAGEN offer a design of Custom PyroMark CpG Assays?
FAQ ID -2818
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How are the PyroMark CpG Assays reconstituted?
FAQ ID -2817
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How are the PyroMark CpG Assays shipped and stored?
FAQ ID -2816
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What are the features of PyroMark CpG Assays, for example, in terms of design and validation?
FAQ ID -2821
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What is included in a PyroMark Custom Assay?
FAQ ID -2815
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In which format can PyroMark CpG Assays be ordered?
FAQ ID -2824
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Which kits can be used in combination with the PyroMark CpG Assays and PyroMark Custom Assays?
FAQ ID -2822
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Does the PyroMark Assay Design or application software give any cycling conditions for individual assay primers or a PCR setup pipetting scheme?
FAQ ID -2862
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What is the recommended amplicon size for CpG assays?
FAQ ID -2825
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What kind of reading length can I expect when using pyrosequencing technology for sequence analysis?
FAQ ID -2216
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Will dUTP in a PCR reaction affect pyrosequencing?
FAQ ID -2843
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Which purity grade is recommended for pyrosequencing primers?
FAQ ID -2832
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What concentration should be used for the sequencing primer in pyrosequencing?
FAQ ID -2826
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What is the concentration of PyroMark Control Oligo?
FAQ ID -2846
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What is the sample throughput of pyrosequencing systems?
FAQ ID -2215
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How do I reduce background peaks in the pyrosequencing pyrogram?
FAQ ID -2877
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What is the sensitivity limitation for pyrosequencing?
FAQ ID -2840
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What should be the single peak height for the PyroMark Control Oligo on the different PyroMark instruments?
FAQ ID -2852
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Which end of the PCR primer for pyrosequencing should be biotinylated?
FAQ ID -2839
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How many nucleotides of a homopolymer can be resolved in pyrosequencing?
FAQ ID -2871
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Where can I order the Streptavidin Sepharose beads for pyrosequencing?
FAQ ID -2850
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What are critical steps during CTC enrichment?
3830
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How pure are the CTCs enriched from blood samples using the AdnaTest?
3831
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Can frozen blood be used for the procedure?
3832
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Can the EZ2 AdnaTest be used for other body fluids than blood?
3833
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Can Heparin blood be used?
3834
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Can the kit be used on the EZ2 Connect?
FAQ ID - 3845
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Can I use a different elution buffer?
FAQ ID - 3847
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What is the average amount of cfDNA present in 1 ml plasma?
FAQ ID - 3846
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Can the kit be used on the EZ1 Advanced XL?
FAQ ID - 3844
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Do you have a protocol for purification of cytoplasmic RNA from animal cells?
FAQ ID -1257
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What kits does QIAGEN offer to extract RNA from whole blood?
FAQ ID -304
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What is the composition of Buffer RLT?
FAQ ID -2793
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What is the key technical challenge in isolating high quality RNA from cell or tissue samples?
FAQ ID -2656
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Do you have a protocol for the isolation of RNA from leukocytes in milk?
FAQ ID -952
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What is the maximum binding capacity of RNeasy spin columns?
FAQ ID -290
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Can QIAquick Kits be used to clean up RNA samples?
FAQ ID -490
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Are RNeasy spin columns sold separately?
FAQ ID -159
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Can the RNase-Free DNase Set be used for DNase digestions of RNA in solution?
FAQ ID -619
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Which kit should be used to extract RNA from adipose tissue, brain, and other fatty animal tissues?
FAQ ID -467
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How can I minimize degradation of RNA from my pancreas sample?
FAQ ID -624
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What is the composition of Buffer RW1?
FAQ ID -2796
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Do you have a protocol for purification of total DNA from crude lysates?
FAQ ID -1255
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Do you have a protocol for isolation of genomic DNA from saliva and mouthwash?
FAQ ID -917
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Do you have a protocol for purification of total DNA from yeast?
FAQ ID -1253
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Do you have a protocol for the isolation of DNA from soft tissues using the TissueLyser?
FAQ ID -924
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Do you have a protocol for purification of total DNA from insects?
FAQ ID -1254
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What is the shelf-life for QIAGEN Proteinase K (cat. no. 19131, 19133)?
FAQ ID - 3447
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Do you have a protocol for the isolation of genomic DNA from sperm?
FAQ ID -909
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What is the advantage of running an analytical gel with fractions of my plasmid preparation?
FAQ ID -769
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What is the recipe for LB?
FAQ ID -212
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How do I know if my plasmid is a high- or low copy number type?
FAQ ID -350
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Can I eliminate RNase A from buffer P1 for my plasmid preparation to obtain RNase-free DNA for in-vitro transcription?
FAQ ID -366
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I left Buffer P1 at room temperature after addition of RNase A, what shall I do?
FAQ ID -859
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With LyseBlue reagent for lysis control, can I now process more bacterial culture and overload the columns?
FAQ ID -864
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What is the recipe for 2x YT?
FAQ ID -213
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What are the additional plasmid bands I see on my gel?
FAQ ID -1059
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Do you have a protocol for the isolation of plasmid DNA from Bacillus subtilis?
FAQ ID -896
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What is the composition of Buffer PB?
FAQ ID -2791
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Do you have a protocol for obtaining > 20 µg plasmid DNA using the QIAprep Spin Miniprep Kit?
FAQ ID -3102
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Why is my plasmid DNA yield low?
FAQ ID -768
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Why do I get genomic DNA contamination in my plasmid prep?
FAQ ID -353
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Do you have a protocol for the isolation of plasmid DNA from Agrobacterium?
FAQ ID -898
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I am seeing a precipitate after adding LyseBlue reagent to Buffer P1. What should I do about that?
FAQ ID -1045
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What to do if cell clumps are present after Buffer P2 addition when using LyseBlue Reagent?
FAQ ID -861
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What is the recipe for SOC medium?
FAQ ID -798
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How much RNA does a typical mammalian cell contain?
FAQ ID -2946
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Do I have to discard Buffer RLT with beta-Mercaptoethanol (ß-ME) added to it after 1 month of storage?
FAQ ID -1037
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What are the effects of low A260/A230 ratios in RNA preparations on downstream applications?
FAQ ID -2248
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Do I need to purchase any consumable for processing the kit on EZ2 Connect?
FAQ ID - 3849
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Are we able to customize protocol on the EZ2 Connect?
FAQ ID - 3850
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Which version of the EZ2 Connect can run the EZ2 RNA/miRNA Tissue/Cells Kit?
FAQ ID - 3851
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Can the kit be run on EZ1 instruments?
FAQ ID - 3852
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How can I check the integrity of RNA purified using RNeasy Kits?
FAQ ID -1024
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What are the limits of detection of the QIAxcel Connect?
FAQ ID - 3853
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Are all QIAxcel Cartridge Kits compatible with QIAxcel Connect?
FAQ ID - 3856
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