| Cell Line Species/Tissue: |
Human
/
Human Bladder |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
20/12,EGF,GlutamineITS,T3,HC, |
Percent Serum (%):
|
|
Reporter System:
|
GFP |
Plasmid Purification Method:
|
QIAGEN Plasmid Kit |
Plate Format:
|
6-well plate |
Number of Cells:
|
|
Percent Confluence(%):
|
10% |
Amount of Nucleic Acid (µg):
|
2.0µg |
Amount of Enhancer (µl):
|
16µl |
Amount of Reagent (µl):
|
20µl |
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
48h |
Transfection Efficiency (%):
|
90% |
| |
| Any modifications to the protocol?:
|
| Modified the DNA - Effectene concentration, and the time of incubation of the Enhancer,DNA-Effectene complexes. |
| Notes: |
| Modified the DNA - Effectene concentration, and the time of incubation of the Enhancer,DNA-Effectene complexes. |
| References: |
| |