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3224 - How much time can be saved when using the RNeasy Mini QIAcube Kit?
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3289-How does the QuantiFast Probe RT-PCR Plus Kit eliminate genomic DNA contamination?
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3290-How do I analyze the data for the particular iPSC qBiomarker Arrays I have ordered?
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3288-How does the gDNA eliminator solution of the RNeasy Plus Universal Kit work?
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3294-How long can I store my purified DNA/RNA isolated with the AllPrep DNA/RNA FFPE kit?
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3286-How can I adjust the ROX concentration in the QuantiFast Probe RT-PCR Plus master mix?
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3291-How do I harvest the crystals from meso phase? Are special tools needed with the NexTal Cubic Phase products?
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3246 - What is the correct matrix to use with Investigator ESSplex SE Plus Kit?
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3297-Why does the PCR for HLA-DRB4 result in 2 amplicons of different size?
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3266 - Why are two different exon 2 forward sequencing primers used for the SBTexcellerator HLA-DRB1 Kit?
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How can I find out if my gene of interest is expressed in a specific tissue type or cell line?
FAQ ID - 3214
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3215 - What is the starting material for SBTexcellerator HLA Kits?
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Can I use genomic DNA from fixed samples with qBiomarker Copy Number PCR Arrays?
FAQ ID — 3416
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Can I use amplified genomic DNA with qBiomarker Copy Number PCR Arrays?
FAQ ID — 3415
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Do the samples require pretreatment before starting the manual protocol or before loading onto the QIAcube instrument?
FAQ ID - 3479
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What are the expected yields of RNA from PAXgene Blood RNA Tubes isolated with the PAXgene Blood miRNA kit?
FAQ ID - 3481
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Do you have stability data for RNA isolated with PAXgene miRNA Kit?
FAQ ID - 3480
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Can the PAXgene Blood DNA Tubes be stored at 4°C?
FAQ ID - 3493
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How should I thaw frozen PAXgene Blood DNA Tubes?
FAQ ID - 3495
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Can the PAXgene Blood DNA Tube be frozen? If yes, at what temperatures and for how long?
FAQ ID - 3491
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Can mitochondrial DNA be isolated with the PAXgene Blood DNA System?
FAQ ID - 3498
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3497 - What is the expected purity and size of DNA extracted using the PAXgene Blood DNA kit?
FAQ ID - 3497
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Does the DNA extracted using the PAXgene Blood DNA System contain RNA?
FAQ ID - 3499
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Can I multiplex the Microbial DNA qPCR Assays?
FAQ ID — 3408
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Can I measure virulence factor gene expression?
FAQ ID — 3405
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What sample types can I test on qBiomarker Copy Number PCR Arrays?
FAQ ID — 3423
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What is the sensitivity for the Microbial DNA qPCR kits?
FAQ ID — 3409
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Which probe labels are available for the Microbial DNA qPCR Assays?
FAQ ID — 3407
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Are the Microbial DNA qPCR Assays wet-lab verified?
FAQ ID — 3406
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Can I measure antibiotic resistance gene expression?
FAQ ID — 3404
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What sequences are used to design the Microbial DNA qPCR Assays?
FAQ ID — 3403
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Are the assays species-specific?
FAQ ID — 3400
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Why is the copy number call lower than expected?
FAQ ID — 3413
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Will qBiomarker Copy Number Assays work with heterogeneous samples like mixtures of tumor and normal tissue?
FAQ ID — 3419
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Why do qBiomarker Copy Number PCR Arrays have assays in quadruplicate?
FAQ ID — 3414
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How should I analyze the data generated from a qBiomarker Copy Number PCR Array experiment?
FAQ ID — 3421
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What could have gone wrong if the CT values are unusually high for all wells in a sample?
FAQ ID — 3412
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What qPCR mastermix should I use with the qBiomarker Copy Number PCR Arrays and Assays
FAQ ID — 3422
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How much DNA should I use on a qBiomarker Copy Number PCR Array?
FAQ ID — 3417
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How much DNA should I use in a qBiomarker Copy Number PCR Assay?
FAQ ID — 3418
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How do you choose which assays to include on the arrays?
FAQ ID — 3420
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What testing should be performed in order to assess the quality of a DNA sample?
FAQ ID — 3424
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How many samples can be processed with the PAXgene Blood miRNA Kit?
FAQ ID - 3477
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Why have the hazard symbols accompanying products changed?
FAQ ID - 3430
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Why is a blood collection set required to be used with the PAXgene Blood RNA tube?
FAQ ID - 3459
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As part of my RT procedure I routinely heat an aliquot of the eluate prior to the RT reaction. Do I still need to heat the whole eluate after the RNA preparation?
FAQ ID - 3468
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Is there any chance of the PAXgene Blood RNA tube reagent going back into the patient's arm?
FAQ ID - 3460
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I need to buy a new computer for my PyroMark Q96. Can I reinstall the PyroMark Q96 software on my new computer or do I need to purchase a new license?
FAQ ID - 3472
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Can I reinstall the PyroMark Q24 software on my new computer or following an operating system upgrade, or do I need to purchase a new license?
FAQ ID - 3473
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Can the PAXgene Blood DNA Tubes be stored at higher temperatures?
FAQ ID - 3494
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What are the expected yields of RNA from PAXgene Blood RNA Tubes using the QIAsymphony PAXgene Blood RNA Kit?
FAQ ID - 3475
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What additional equipment is needed to purify RNA using the PAXgene 96 Blood RNA Kit?
FAQ ID - 3474
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Is it possible to isolate genomic DNA or viral nucleic acids with the QIAsymphony PAXgene Blood RNA protocol?
FAQ ID - 3476
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2. Are there any special considerations for the collection of blood using PAXgene Blood DNA Tubes CE (e.g., use of a blood collection set or discard tube, positioning of the tube, issues with short draws, etc.)?
FAQ ID - 3485
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Are the PAXgene Blood DNA Tubes sterile?
FAQ ID - 3487
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What is the additive in PAXgene Blood DNA Tubes
FAQ ID - 3486
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What is the maximum number of samples for the QIAcube variant of the PAXgene Blood miRNA protocol for a single run?
FAQ ID - 3482
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Do I have to use a blood collection set with the PAXgene Blood DNA Tube?
FAQ ID - 3484
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Is it possible to isolate genomic DNA or viral nucleic acids with PAXgene Blood miRNA protocol?
FAQ ID - 3483
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How should blood samples drawn into PAXgene Blood DNA Tubes be shipped?
FAQ ID - 3489
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How long can the PAXgene Blood DNA Tube be kept at room temperature? What is room temperature?
FAQ ID - 3492
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Which sample types can be processed using the PAXgene Blood miRNA protocols?
FAQ ID - 3478
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What is the shelf life of the PAXgene Blood DNA Tube prior to blood collection?
FAQ ID - 3488
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How should blood samples drawn into the PAXgene Blood DNA Tubes be stored?
FAQ ID - 3490
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Can the miScript II RT Kit be used to quantify piRNAs?
FAQ ID - 3432
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Can the miScript Plant RT Kit be used to quantify piRNA expression?
FAQ ID - 3434
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Can the miScript Plant RT Kit be used to quantify animal miRNAs?
FAQ ID - 3433
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Does the miScript Plant RT Kit contain controls?
FAQ ID - 3435
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What is the composition of the 5x miScript Plant RT Buffer?
FAQ ID - 3437
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Are any of the miScript II RT Kit components interchangeable with the miScript Plant RT Kit components?
FAQ ID - 3436
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What should I use to isolate total RNA from plant tissues and what is the maximum recommended amount of tissue input?
FAQ ID - 3438
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Are miScript Primer Assays available for plant mature miRNAs?
FAQ ID - 3439
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Are plant miScript Primer Assay designs wet-bench validated?
FAQ ID - 3440
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Can you describe the adaptor found in the 10x miScript Plant Adaptor?
FAQ ID - 3442
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Are miScript Primer Assay included in miScript miRNA PCR Arrays the same primers as the miScript Primer Assays available in individual tubes?
FAQ ID - 3441
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What is the amplicon size of PCR products generated with the miScript Plant qPCR System?
FAQ ID - 3443
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What is the recommended RNA and RNA input range for the miScript Plant RT Kit?
FAQ ID - 3446
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Can cDNA synthesized with the miScript Plant RT Kit be used in real-time PCR with TaqMan® MicroRNA Assays?
FAQ ID - 3445
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Can the PAXgene RNA stabilization solution/blood mixture from the PAXgene Blood RNA Tube be disposed of by adding bleach?
FAQ ID - 3469
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Can I scale up reagents if I want to use more sample with the MagAttract HMW DNA Kit (cat. no. 67563)?
FAQ ID - 3448
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How can I calculate the number of bacterial cells that are present in a sample using the Microbial DNA qPCR Assays?
FAQ ID — 3411
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What is the difference between the Microbial DNA qPCR Assays and the QuantiFast Pathogen +IC Kits?
FAQ ID — 3410
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What RNA yields are expected with the PAXgene Blood RNA kit?
FAQ ID - 3464
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Can the PAXgene 96 Blood RNA Kit be used for diagnostic or prognostic procedures?
FAQ ID - 3471
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What are possible reasons for blood draws with lower than expected blood volume?
FAQ ID - 3462
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How long can the PAXgene Blood RNA tubes be kept frozen at –20°C or –70°C?
FAQ ID - 3463
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Can the PAXgene Blood RNA System be used to isolate DNA?
FAQ ID - 3466
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Can the PAXgene Blood RNA System be used to isolate viral RNA?
FAQ ID - 3465
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Can the PAXgene Blood RNA System be used with animal blood samples?
FAQ ID - 3461
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Can I use DNA isolated from an AllPrep DNA/RNA Kit with qBiomarker Copy Number PCR Arrays and Assays
FAQ ID — 3425
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What is the Globally Harmonized System of Classification and Labelling of Chemicals (GHS) and what is the impact for me as a customer?
FAQ ID - 3428
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What transfection method should I use with the SureSilencing shRNA Plasmids?
FAQ ID -2901
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What are the software requirements to run the QIAlink software package?
FAQ - 3426
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What is the data analysis method, and where can I find it for the qBiomarker Somatic Mutatation PCR arrays?
FAQ ID -2915
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What is the nature and target of the Internal Control supplied with Certal Residual DNA Detection Kits?
FAQ ID -2974
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What is the principle for qBiomarker Somatic Mutation PCR Array data analysis?
FAQ ID -2916
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What is the label of the probe that is used in the Internal Control Assay to detect the Internal Control supplied with Certal Residual DNA Detection Kits?
FAQ ID -2976
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Were all assays on the qBiomarker Somatic Mutation PCR Arrays bench-validated?
FAQ ID -2912
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What are the advantages of RT-PCR based somatic mutation PCR arrays and assays compared to other platforms?
FAQ ID -2921
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What are the control features on the qBiomarker Somatic Mutation PCR Arrays?
FAQ ID -2914
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What happens if the starting volume is too low when using QIAsymphony mericon Bacteria Kit?
FAQ ID -2962
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What happens if the starting volume is too high when using QIAsymphony mericon Bacteria Kit? Can spillage occur when the sample is initially transferred?
FAQ ID -2963
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What effects can be predicted with poor sample quality? How robust is the platform with questionable sample quality (such as FFPE samples)?
FAQ ID -2918
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What elution formats can be used for the QIAsymphony PAXgene Blood RNA protocol?
FAQ ID -2986
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What elution formats can be used for the QIAsymphony DSP Virus/Pathogen protocols?
FAQ ID -2937
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When should I choose EasyXpress Disulfide Insect Kits?
FAQ ID -2971
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Which elution volumes can be used for the QIAsymphony DSP Virus/Pathogen protocols?
FAQ ID -2938
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What sample volumes can be used with the tissue HC and LC protocols on the QIAsymphony SP?
FAQ ID -2992
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What types of templates can be used for expression of antibody fragments?
FAQ ID -2964
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Why do I have to use an ACS (Assay Control Set)?
FAQ ID -2933
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Which kit size should be used for each QIAsymphony DSP Virus/Pathogen protocol?
FAQ ID -2930
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Will mitochondrial DNA also be isolated using the DNA tissue protocols and QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP?
FAQ ID -2991
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Which sample types can be processed using the QIAsymphony PAXgene Blood RNA Protocol?
FAQ ID -2981
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What pathways and diseases are currently covered for the qBiomarker Somatic Mutation PCR Arrays?
FAQ ID -2922
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Why does the template for EasyXpress Disulfide Insect Kits need to have a signal sequence (such as the mellitin signal sequence as provided by the EasyXpress Linear Template Fab Kit primers)?
FAQ ID -2967
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What sample input formats can be used for the QIAsymphony DSP Virus/Pathogen protocols?
FAQ ID -2936
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What is the starting volume for the QIAsymphony mericon Bacteria Kit?
FAQ ID -2956
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What is the Limit of Detection (LOD) for an ELISArray Kit?
FAQ ID -2907
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What is the sensitivity of the assays contained on the qBiomarker Somatic Mutation PCR Arrays?
FAQ ID -2910
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What is the linear range of the Multi-Analyte ELISArray Kits?
FAQ ID -2909
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What are the expected DNA yields from tissue processed on the QIAsymphony SP?
FAQ ID -2990
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What is the maximum number of GOIs that I can detect with the Type-it CNV Probe Master Mix in the same qPCR reaction?
FAQ ID -2998
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What are the recommended lysis times for tissue samples on the QIAsymphony SP?
FAQ ID -2993
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What are the technological principles behind somatic mutation PCR arrays and assays?
FAQ ID -2924
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What PCR machine do I need to use to run such a qBiomarker Somatic Mutation PCR Arrays?
FAQ ID -2917
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What method(s) do you recommend as an alternative way to validate the mutations identified on the somatic mutation PCR arrays?
FAQ ID -2920
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What sample types can I test on the qBiomarker Somatic Mutation PCR Arrays?
FAQ ID -2919
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What sample volumes can be processed using the QIAsymphony DSP Virus/Pathogen protocols?
FAQ ID -2929
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What sample types should be processed with the low content (LC) and high content (HC) protocols for DNA extraction on the QIAsymphony SP?
FAQ ID -2989
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What samples can the QIAsymphony mericon Bacteria Kit be used for?
FAQ ID -2958
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What sample types can be processed using the QIAsymphony DSP Virus/Pathogen protocols?
FAQ ID -2928
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Do you have stability data for RNA isolated with the QIAsymphony PAXgene Blood RNA Kit?
FAQ ID -2983
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What sample types can be processed using the tissue protocols and the QIAsymphony DSP DNA Mini Kit?
FAQ ID -2925
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Do you need to pre-treat food samples before DNA isolation using the QIAsymphony mericon Bacteria Kit on the QIAsymphony SP?
FAQ ID -2957
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Do the samples require pretreatment before loading onto the instrument with the QIAsymphony PAXgene Blood RNA Protocol?
FAQ ID -2982
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How do you decide on the qBiomarker Somatic Mutation PCR Arrays to include on the pathway arrays and disease arrays?
FAQ ID -2913
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Do the SureSilencing shRNA Plasmids contain inducible promoters?
FAQ ID -2904
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How many freeze-thaw cycles can the Internal Control supplied with Certal Residual DNA Detection Kits tolerate?
FAQ ID -2975
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How many mutations can I profile per sample on the qBiomarker Somatic Mutation PCR Array?
FAQ ID -2911
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How can I estimate the size of my protein based on the length of the respective cDNA?
FAQ ID -2952
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How do I use the qBiomarker Somatic Mutation PCR Arrays?
FAQ ID -2923
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Can I use the Type-it CNV kits and Reference Assays for CNV studis in mice?
FAQ ID -2997
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Can the Certal CHO Positive Control DNA be used to create a qPCR standard for relative quantification?
FAQ ID -2979
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Can I use lysates or tissue homogenates with the ELISArray kits?
FAQ ID -2905
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Can the EasyXpress Linear Template Fab Kit be used to express entire IgGs?
FAQ ID -2966
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Can I use TaqMan® Copy Number Assays from LIFE Technologies in combination with the Type-it CNV Probe kit?
FAQ ID -2999
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Can I use the SureSilencing shRNA Plasmids with primary cells or macrophages or for injection into live animals?
FAQ ID -2902
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Do I have to use an internal control with each QIAsymphony DSP Virus/Pathogen Kit protocol run?
FAQ ID -2932
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Do samples require pretreatment before they are loaded onto the QIAsymphony instrument?
FAQ ID -2931
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Can the QIAsymphony DSP Virus/Pathogen system be used for diagnostic or prognostic procedures?
FAQ ID -2939
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Do the ELISArray Kits work on species other than human, mouse or rat?
FAQ ID -2906
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Does the QIAsymphony mericon Bacteria Kit contain an extraction control?
FAQ ID -2961
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Is it possible to simultaneously isolate DNA and RNA from Saliva Stabilized in RNAprotect® Saliva Reagent?
FAQ ID -2996
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Is it possible to use templates with signal sequences with EasyXpress Disulfide E. coli Kits?
FAQ ID -2969
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Is food DNA co-purified using the QIAsymphony mericon Bacteria Kit?
FAQ ID -2959
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Is it possible to introduce an affinity tag using the EasyXpress Linear Template Fab Kit?
FAQ ID -2972
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Is it possible to isolate DNA from Saliva Stabilized in RNAprotect® Saliva Reagent?
FAQ ID -2995
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On what cyclers have Certal Residual DNA Detection Kits been validated?
FAQ ID -2978
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On which cyclers can Certal Residual Detection Kits be used?
FAQ ID -2973
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Should I use the SureSilencing shRNA Plasmids with the GFP, neomycin, or hygromycin, or puromycin resistance marker?
FAQ ID -2900
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Is it possible to use the native signal sequence of antibodies with EasyXpress Disulfide Insect Kits?
FAQ ID -2968
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Is RNA co-purified using the QIAsymphony mericon Bacteria Kit?
FAQ ID -2960
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How much DNA does a bacterial cell contain?
FAQ ID -2947
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How many samples can be processed with the QIAsymphony DSP Virus/Pathogen Kits?
FAQ ID -2935
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How many reactions can be performed with one QIAsymphony mericon Bacteria Kit?
FAQ ID -2955
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How should the samples be stored before purification of RNA with the QIAsymphony PAXgene Blood RNA Protocol?
FAQ ID -2984
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How should the samples be stored before purification with the QIAsymphony DSP Virus/Pathogen Kits?
FAQ ID -2934
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If a cycler from Applied Biosystems is used in combination with the Certal Residual DNA Detection Kit, should dye calibration for MAX be performed?
FAQ ID -2977
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How much sample is required for each well for an ELISArray Kit?
FAQ ID -2908
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How much starting material can be used with the QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP?
FAQ ID -2927
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3198 - What is the optimal DNA concentration for the Investigator DIPlex Kit?
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3194 - What DNA fragment sizes are required for the Investigator DIPlex kit?
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3195 - Where can I find the Population Data for the Investigator DIPlex kit?
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3196 - What is the DIPsorter Freeware?
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3188 - How many His residues are required for successful purification on Ni-NTA resins?
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3190 - What is the smallest elution volume for RNeasy Mini Spin column?
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Why does the QIAcube sometimes fail to pick up the tips or pick up wrong tips?
FAQ ID -3131
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The kit says I can use up to 30mg of tissue. When I used 15mg I got a higher yield than when I used 30mg. Why?
FAQ ID -3117
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I want to purify RNA from heart tissue. What is the best kit to use?
FAQ ID -3116
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Why does the upper aqueous phase look pinkish when purifying RNA from fatty tissue?
FAQ ID -3118
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Is it possible to partially use RNeasy 96 plates and use the plate multiple times?
FAQ ID -3124
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Can I use the QIAGEN deparaffinization solution with the IDH 1/2 RGQ PCR kit?
FAQ ID -3100
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Can I use stained slide materials with IDH 1/2 RGQ PCR kit?
FAQ ID -3101
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What is the standard format for Flexiplate siRNAs delivery?
FAQ ID -3104
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How many transfections can be performed with Flexiplate siRNAs?
FAQ ID -3105
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Where to look for ordering Custom siRNAs online?
FAQ ID -3106
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What is the difference between HiPerFect and HiPerFect HTS?
FAQ ID -3108
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What are the miScript Controls that can be used with miScript HiSpec Buffer or miScript HiFlex Buffer?
FAQ ID -3111
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What is the amplicon size of the PCR product when using the miScript primer assay?
FAQ ID -3156
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What is the difference between EpiTect Bisulfite Kit and EpiTect Plus Bisulfite Kit?
FAQ ID -3167
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What are the stopping points in the QIAamp protocol?
FAQ ID -3157
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What are the benefits of PyroMark Q24 Advanced versus PyroMark Q24?
FAQ ID -3158
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Do you have data on the DNA yield at different incubation time for the Repli-G Kits?
FAQ ID -3113
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Is it possible to upgrade existing PyroMark Q24 instruments to PyroMark Q24 Advanced or do I have to purchase a new instrument?
FAQ ID -3162
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Is it possible to run therascreen mutation or therascreen Pyro kits on PyroMark Q24 Advanced system?
FAQ ID -3161
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Which operating system is compatible with PyroMark Q24 Advanced application software?
FAQ ID -3164
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What is the adapted annealing protocol for PyroMark Q24 Advanced procedure?
FAQ ID -3166
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What is the miRNA yield from the miRNeasy Serum/Plasma Kit?
FAQ ID -3171
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What is the RNA size distribution recovered from the miRNeasy Serum/Plasma Kit?
FAQ ID -3172
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What could be a reason for higher viscosity of PCR reaction with STR Plus chemistry?
FAQ ID -3176
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Does QIAGEN provide template files for GeneMapper® 5 software?
FAQ ID -3177
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Can Rotor-Gene Reports be modified?
FAQ ID -3184
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What is the best way to ensure the Rotor-Disc is properly sealed when using the Heat Sealer?
FAQ ID -3179
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What to do if there is a loss of communication for the Rotor-Gene Q?
FAQ ID -3180
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Aside from the OTV test, what other maintenance should be done for the Rotor-Gene Q?
FAQ ID -3181
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Are the miRNA Target Protector & miRNA mimic bench tested? Any guarantee?
FAQ ID - 3186
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3187 - Why does the Ni-NTA resin turn reddish-brown after mixing with the lysates?
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3191 - Is the Allprep DNA Mini Spin Column the same as the gDNA eliminator column? Can I interchange these columns?
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3199 - Which species can be used with the GeneRead rRNA depletion kit?
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3192 - Is QIAGEN Protease compatible with Buffer ATL?
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What is the bacterial ribosomal RNA recommended to use in the miRNeasy Serum/Plasma Protocol?
FAQ ID -3173
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3193 - Where can I get an RNase-free syringe that is recommended for reconstituting QIAGEN's RNase-free DNase (cat. no. 79254)?
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Would co-transfection with the plasmid be expected to increase/decrease the transfection efficiency significantly?
FAQ ID -3136
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How can I know whether there is any genomic DNA present in my purified plasmid?
FAQ ID -3134
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How do I know whether my miRNA has been successfully purified and how much is expected?
FAQ ID -3135
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Is it possible to use RNAprotect Cell Reagent to stabilize buffy coat?
FAQ ID -3139
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Has QIAGEN ever tested Proteinase K treatment for a time period longer than overnight?
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Is RNAprotect also suitable for the stabilization of miRNA?
FAQ ID -3143
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Does RNAprotect affect morphology of tissues?
FAQ ID -3142
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Are miScript primer assays bench-validated?
FAQ ID -3145
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What is the maximum cDNA length that can be synthesized by QuantiTect Reverse Transcription Kit?
FAQ ID -3146
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Why does the DNeasy mericon Food extraction kit use a QIAquick column and not DNeasy column?
FAQ ID -3148
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What are the minimal and maximum fragment sizes for the QIAquick column in the mericon DNeasy kit?
FAQ ID -3147
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Does RNase-free DNase I affect the isolation of dsRNA?
FAQ ID -3153
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Can single-stranded DNA be removed by gDNA Eliminator columns?
FAQ ID -3152
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How is total RNA purified with RNeasy kits free of genomic DNA contamination?
FAQ ID -3154
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Is it possible to quantify protein content in RLT lysates?
FAQ ID -3155
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What primary cell lines have been tested with HiPerFect Transfection Reagent?
FAQ ID -3137
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Is it possible to use the protein resuspended in ALO buffer for downstream BCA assay and Bradford assay?
FAQ ID -3138
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What are the miScript Controls that can be used with miScript HiFlex Buffer ONLY?
FAQ ID -3112
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How long can I store an RLT lysate?
FAQ ID -3115
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Do you have a protocol for the isolation of genomic DNA from yeast/fungi directly from Blood?
FAQ ID -3150
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Is there cross talk between channels in the Rotor-Gene Q?
FAQ ID -3185
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3197 -Where can I find the Population Data for the Investigator HDplex kit?
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Can QIAzol aqueous phase be used with the gDNA eliminator columns in RNeasy Plus mini kit?
FAQ ID -3151
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What is in the HiSpec Buffer that selectively synthesizes cDNA from mature miRNAs ONLY?
FAQ ID -3168
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How do I prepare the Spike-in-Control catalog # 219610 to get copies/μl?
FAQ ID -3170
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What is the optimal DNA concentration for the Investigator ESSplex Plus Kit?
FAQ ID -2646
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Why do I see low, poor, or sub-standard amplification efficiency in my qRT-PCR assay?
FAQ ID -2695
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What is the difference between adding the Internal Control (IC) template used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit to the amplification reaction versus adding the IC template at the extraction step?
FAQ ID -2600
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Is the QuantiFast SYBR Green PCR Kit compatible with the ViiA7 cycler from Applied Biosystems?
FAQ ID -2652
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What are the most reliable methods for preparing high-quality RNA from cell or tissue samples, for use in gene expression analysis experiments?
FAQ ID -2657
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What should the cycler set-up be for a duplex reaction with the pathogen assay (FAM) and the Internal Control assay (MAX) on ABI instruments, when using the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2610
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What should the cycler set-up be for a duplex reaction with the pathogen assay (FAM) and the Internal Control assay (MAX) on Mx instruments from Stratagene (Agilent), when using the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2609
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What should I do if I suspect that my RNA preparation contains RNase contamination?
FAQ ID -2661
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What real-time cycler should I use for my qPCR experiments?
FAQ ID -2670
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Which DNA/RNA extraction kits were tested in combination with the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2606
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What should the Rotor-Gene Q cycler settings be for a duplex reaction with the pathogen assay (FAM) and the Internal Control assay (MAX), when using the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2608
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What testing should be performed to assess the quality of an RNA sample?
FAQ ID -2660
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For the QuantiFast Pathogen RT-PCR +IC kit, does the Internal Control also control for the reverse transcription reaction?
FAQ ID -2601
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Do we have data on long term stability and storage of DNA purified on BioRobot M48?
FAQ ID -2611
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Following thermal cycling, reaction volume has evaporated from some of the wells of my qPCR assay plate. What should I do?
FAQ ID -2683
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How can I predict the percent qPCR signal due to contaminating DNA, for a given qPCR assay, and its matching NRT control?
FAQ ID -2688
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How can I ensure that reaction volume is not lost due to evaporation during thermal cycling?
FAQ ID -2679
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How can I determine whether amplification occurs from mRNA-derived cDNA or from genomic DNA contamination?
FAQ ID -2687
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Can the Internal Control DNA or RNA be added directly to the sample?
FAQ ID -2602
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Do the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit contain ROX in the master mix?
FAQ ID -2605
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Are primers available that only detect mitochondrial DNA encoded genes and not nuclear genomic DNA encoded genes?
FAQ ID -2680
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Do I need to run a standard curve before the actual PCR array experiment?
FAQ ID -2664
View
Does the amount of Internal Control to be spiked into the lysis buffer or lysate depend on the elution volume only?
FAQ ID -2604
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May I try the data analysis tool without using your PCR array kit?
FAQ ID -2698
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Is the QuantiFast Multiplex PCR +R Kit compatible with the ViiA7 cycler from Applied Biosystems?
FAQ ID -2653
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Is the QuantiFast Probe PCR +ROX Vial Kit compatible with the ViiA7 cycler from Applied Biosystems?
FAQ ID -2651
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Is it good to pool multiple RNA replicates to detect expression changes that are consistently reproducible?
FAQ ID -2663
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How important is the RNA purification process, for obtaining reliable qRT-PCR results?
FAQ ID -2655
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Is a special processing protocol needed?
FAQ ID -2523
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Is it possible to archive PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue blocks?
FAQ ID -2524
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The disruption of my sample worked well with the TissueLyser LT, but I am not satisfied with the RNA yield. What do you suggest?
FAQ ID -2555
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What are the dimensions of the TissueLyser LT?
FAQ ID -2545
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What is the average RNA yield from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -2534
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On which cyclers can the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit be used?
FAQ ID -2596
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What are the storage conditions for the mericon Assay kits?
FAQ ID -2540
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What is in the elution buffer in the PAXgene Bone Marrow RNA kit?
FAQ ID -2511
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Is it possible to microdissect PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissues?
FAQ ID -2531
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Is the performance of the HiPerFect HTS Reagent affected if it has accidentally been stored at°C?
-20
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Is it possible to embed PAXgene Tissue fixed and stabilized samples in Optimal Cutting Temperature (OCT) medium for freezing?
FAQ ID -2525
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Is the morphology after H&E staining comparable to formalin-fixed samples?
FAQ ID -2526
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What RNA yields are expected with the PAXgene Bone Marrow RNA System?
FAQ ID -2506
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What is the maximum tissue size that can be fixed in a PAXgene Tissue Container?
FAQ ID -2518
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What is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?
FAQ ID -2538
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What is the expected amplicon size when using the mericon Assay kits?
FAQ ID -2544
View
What is the typical amount of tissue used for disruption with the TissueLyser LT?
FAQ ID -2552
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What is the longest period you recommend to leave out the dilute HiPerFect HTS at RT before use?
FAQ ID -2560
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What kind of sample tubes are recommended to use with the TissueLyser LT?
FAQ ID -2553
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What is the purity of nucleic acids extracted with the PAXgene Tissue kits?
FAQ ID -2533
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Can the PAXgene Bone Marrow RNA System be used to isolate genomic DNA or viral RNA and DNA?
FAQ ID -2507
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Can the PAXgene Bone Marrow RNA System be used with animal bone marrow or with blood samples?
FAQ ID -2501
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Can the PAXgene RNA stabilization solution/bone marrow mixture from the PAXgene Bone Marrow RNA Tube be disposed of by adding bleach?
FAQ ID -2515
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Can sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for other histochemical staining techniques, such as PAS?
FAQ ID -2530
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Can the PAXgene Bone Marrow RNA System be used for other human body fluids than bone marrow samples or cultured cells of human origin?
FAQ ID -2502
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Can the sample tubes and the TissueLyser LT Adapter be cooled with liquid nitrogen?
FAQ ID -2549
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Can the TissueLyser LT Adapter be removed for cleaning?
FAQ ID -2546
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Can the samples on the TissueLyser LT be protected from degradation of nucleic acids or proteins?
FAQ ID -2548
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When using the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit, what should the fluorescent label of the probe be for the customer-defined assay to detect the pathogen?
FAQ ID -2594
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When using HiPerFect HTS Reagent is it necessary to optimize amount of siRNA and transfection reagent?
FAQ ID -2556
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What sample numbers can be processed on the TissueLyser LT?
FAQ ID -2547
View
What size bead should I use for tougher samples with the TissueLyser LT?
FAQ ID -2554
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When performing the Internal Control assay for the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit, what channel or filter can be used to detect MAX?
FAQ ID -2597
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Why is the fluorescence signal of the Internal Control on my Rotor-Gene Q very low?
FAQ ID -2574
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Can specific types of white blood cells be enriched from a PAXgene Bone Marrow RNA Tube prior to the RNA isolation?
FAQ ID -2508
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Can bone marrow aliquots other than 2 mL be pipetted into the PAXgene Bone Marrow RNA Tube?
FAQ ID -2503
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Are the Internal Controls used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit homologous to a known target?
FAQ ID -2598
View
Can I use water to elute the RNA in the PAXgene Bone Marrow RNA Kit?
FAQ ID -2512
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Can PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for in situ hybridization?
FAQ ID -2529
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Can bone marrow from leukemia patients with elevated numbers of white blood cells be processed with the PAXgene Bone Marrow RNA System?
FAQ ID -2510
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Can HiPerFect HTS be used for miRNA?
FAQ ID -2561
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Can HiPerFect HTS Reagent be used for transfecting shRNAs?
FAQ ID -2559
View
How long does the sample disruption with the TissueLyser LT take?
FAQ ID -2550
View
How many times can the Internal Controls used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit be freeze-thawed?
FAQ ID -2599
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How long can I store the dilutions of the Control DNA Z1 standards at 2°C?
-8
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How long can primer and probes from the mericon Assay kits be kept at°C once reconstituted?
-20
View
How should the PAXgene Bone Marrow RNA Tubes be frozen after collection and for how long?
FAQ ID -2505
View
I routinely heat an aliquot of the RNA eluate prior to my RT reaction. Do I still need to heat the eluate after the PAXgene Bone Marrow RNA preparation?
FAQ ID -2513
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How much time may the aspirated bone marrow sample be allowed to incubate before pipetting it into the PAXgene Bone Marrow RNA Tube?
FAQ ID -2504
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Do you have FACS or Fluoro-Microscopy data using HiPerFect HTS Reagent for delivery of siRNA?
FAQ ID -2557
View
How is stability and performance of these mericon Assay kits guaranteed?
FAQ ID -2542
View
How are the supernatants resulting from the first two spin steps of the PAXgene Bone Marrow RNA kit purification procedure disposed?
FAQ ID -2516
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What are typical yields of bisulfite converted DNA when using the EpiTect Plus Bisulfite Kits?
FAQ ID -2408
View
What batch sizes can be selected with the PAXgene Blood RNA MDx Kit?
FAQ ID -2491
View
What are the labels of the probe which is used in the Internal Control Assay for detection of the IC?
FAQ ID -2449
View
What is the detection limit for the QuantiFast Pathogen + IC kits?
FAQ ID -2453
View
In which solution can I keep my protein for the NexTal Cubic Phase kits?
FAQ ID -2416
View
What is the difference between QuantiTect Virus Kit and the new QuantiFast Pathogen +IC Kits?
FAQ ID -2448
View
Is there a critical step during upfront sample handling when using the PAXgene Blood RNA MDX kit?
FAQ ID -2493
View
Which EpiTect Bisulfite Plus kit do you recommend for blood samples, cells, and tissues?
FAQ ID -2411
View
What is the nature of the Internal Control in the QuantiFast Pathogen + IC kit?
FAQ ID -2450
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Where can I find general information about RT-PCR and PCR arrays such as information about sample preparation, reverse transcription, quantitative PCR, real-time PCR, and PCR arrays?
FAQ ID -2446
View
What is the maximum number of batches for the QIAsymphony PAXgene Blood RNA protocol for a single run?
FAQ ID -2498
View
Why should I work with diluted or undiluted screens?
FAQ ID -2419
View
Why should I use the EpiTect Plus FFPE Bisulfite Kit instead of isolating FFPE DNA first and then doing the bisulfite conversion?
FAQ ID -2412
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