Product FAQ - QIAGEN



Product FAQ Bookmark Compartilhar more.. Você não está autorizado a baixar o recurso Organizar opções Sort alphabetically Sort by relevance What is the dissociation curve temperature of PCR products generated with the miScript Single Cell qPCR System? FAQ ID - 3579 Visualizar When performing real-time PCR quality control associated with the miScript Single Cell qPCR Kit, is it necessary to perform control experiments for all individual samples/cells? FAQ ID - 3575 Visualizar Do you recommend normalizing single cell miRNA expression data to a “housekeeping” gene or miRNA? FAQ ID - 3580 Visualizar How should data be analyzed when the miScript Single Cell qPCR Kit is used with miScript miRNA PCR Arrays or miScript Primer Assays? FAQ ID - 3581 Visualizar What single cell isolation methods are recommended for use with the miScript Single Cell qPCR Kit? FAQ ID - 3582 Visualizar How would I provide information on the QuantiTect Primer assays in publications when the sequence is confidential? FAQ ID - 3593 Visualizar In which tubes is clot detection possible on QIAsymphony? FAQ ID - 3589 Visualizar How can I create a report/logfile on my QIAsymphony SP system? FAQ ID - 3594 Visualizar Is it possible to measure the proteolytic activity of proteins on the surface of exosomes that were isolated with ExoEasy? FAQ ID - 3599 Visualizar Which barcodes on sample tubes are accepted by the QIAsymphony SP? FAQ ID - 3598 Visualizar Can I change the language on my QIAsymphony SP system? FAQ ID - 3595 Visualizar Can I use blood, cells or bacteria with the QIAamp Fast DNA Tissue Kit? FAQ ID - 3587 Visualizar Can the Rotor-Gene Q be connected to LIMS? FAQ ID - 3596 Visualizar Can the REPLI-g Mitochondrial DNA Kit be used for species other than humans? FAQ ID - 3584 Visualizar Which Microbial qPCR Mastermix should I use? FAQ ID — 3395 Visualizar What are the storage conditions for the Microbial DNA qPCR products? FAQ ID — 3394 Visualizar 3327 - How often should the o-ring for the pipettor tip-adapter be changed on the QIAcube? Visualizar 3326 - Which QIAcube standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet? Visualizar 3328 - Can the Sample Tubes CB and the Sample Tubes RB be used interchangeably? Visualizar 3330 - Is there any contamination from E. coli genomic DNA in the polymerase provided with the Repli-G Single Cell Kit? Visualizar 3329 - When using REPLI-g, if the starting material is a single sorted cell, could mitochondrial DNA (mtDNA) be amplified? Visualizar 3331 - Should the REPLI-g amplified mtDNA be cleaned up before sequencing? Visualizar 3332 - Is there a method that can distinguish between non-amplified DNA and REPLI-g amplified DNA? Visualizar 3333 - When using REPLI-g, what is the purpose of the DTT added to the denaturation buffer in the cells/blood protocol? Visualizar What sample types can be tested on the arrays/assays? FAQ ID — 3399 Visualizar What are the minimum sample requirements for Microbial DNA qPCR kits? FAQ ID — 3393 Visualizar Can I use the Microbial DNA-Free Water and Microbial qPCR Mastermix if they have been opened more than 3 times? FAQ ID — 3398 Visualizar Visualizar 3353 - What is the composition of the elution buffer in the Ni-NTA Fast Start Kit? FAQ ID - 3353 Visualizar 3352 - What are the size ranges of Ni-NTA particles? FAQ ID - 3352 Visualizar 3354 - What type and amount of resin is packed into the Ni-NTA Spin columns from the Ni-NTA Spin Kit? FAQ ID - 3354 Visualizar 3341 - How are the EpiTect DNA controls produced and tested? FAQ - 3341 Visualizar 3351 - What is the upper limit for protein size that can bind to Ni-NTA agarose resin? FAQ ID - 3351 Visualizar 3344 - What is the difference between the EpiTect Plus DNA Bisulfite kit compared to the classic kit? FAQ - 3344 Visualizar 3345 - What is the difference between the high and low concentration EpiTect Bisulfite Conversion protocol? FAQ - 3345 Visualizar 3311 - Can QuickLyse miniprep kits be used with QIAVac 24 Plus? Visualizar 3313 - What is the endotoxin level for the QIAGEN Plasmid Plus kits? Visualizar 3312 - For DirectPrep 96 miniprep, is it possible to use a half plate and save the other half for later prep? Visualizar 3314-What is the concentration of RNase A sold separately? Visualizar 3316-What is the concentration of the reconstituted QuantiFast Probe Assay? Visualizar Visualizar 3325 - What are the catalog numbers for the packing materials for the QIAcube? Visualizar 3324 - Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube? Visualizar 3335 - Can I use the DNA directly from enzymatic reaction for Epitect Bisulfite treatment with the Epitect Bisulfite kit (cat. no. 59104)? Visualizar 3336 - Can I use the linearized DNA for Epitect Bisulfite treatment with the Epitect Bisulfite kit? Visualizar 3339 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) negatively impact subsequent NGS sequencing? Visualizar 3337 - Which method is used for QIAGEN sequencing service? Visualizar 3338 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) have 3'-hydroxyl and 5'-phosphate groups? Visualizar 3343 - Why is it difficult to quantify DNA using UV/Vis spectroscopy and fluorescence methods following EpiTect Bisulfite Conversion? How best do you quantify it? FAQ - 3343 Visualizar 3342 - How is the unmethylated unconverted EpiTect DNA control to be used? FAQ - 3342 Visualizar 3315-What is the concentration of the reconstituted QuantiTect Primer Assay (200)? Visualizar 3322 - What is the concentration of the reconstituted miScript Primer Assay (100) and miScript Precursor Assay (100) tubes? Visualizar 3317 - Do you have a protocol for isolation of genomic DNA from cell cultures using the QIAamp cador Pathogen Mini Kit? Visualizar 3318 - What guidelines should I follow in designing primers for use with the EpiTect MSP Kit? Visualizar 3320 - What is the sensitivity of the Type-it HRM PCR Kit? Visualizar 3321 - What is the purpose of the C. elegans miR-39 spike-in control in the miRNeasy Serum/Plasma kit? Visualizar 3318 - What is the preferred amplicon size for performing PCR on DNA amplified with the EpiTect Whole Bisulfitome Kit? Visualizar 3305 - What is the minimal amount of starting material required for using the SBTexcellerator HLA Kits? Visualizar Visualizar Visualizar Visualizar 3306 - What is the optimal sample DNA concentration for the Investigator ESSplex SE Plus Kit? Visualizar 3304 - What is the maximum DNA input volume that can be used in the Investigator ESSplex SE Plus Kit? Visualizar 3310 - Which settings for the filter set Any5Dye must be adjusted before running the spectral calibration for Investigator ESSplex SE Plus Kit? Visualizar 3308 - Where can I find the template files for the Investigator ESSplex SE Plus Kit? Visualizar 3309 - Which filter set should be used with Investigator ESSplex SE Plus Kit? Visualizar What additional equipment is needed to purify RNA using the PAXgene 96 Blood RNA Kit? FAQ ID -499 Visualizar 3300 - Can isopropanol or methanol be used in place of ethanol in RNeasy kits? Visualizar 3301 - Do you have any information or guidelines regarding the choice of reference genes for real-time PCR? Visualizar 3302 - How long can I store my purified RNA? Visualizar 3210 - Can the 4-16K Centrifuge be used with a 208 V outlet? Visualizar 3211 - What is the maximum speed of the plate rotor 2 x 96 in the 4-16 or 4-16K? Visualizar 3212 - What is the minimum size of DNA fragment that can be isolated with QIAamp DNA Mini kit? Visualizar 3299-Can Buffer ER from the Endofree Plasmid kits be used instead of the Buffer ETR in QIAGEN Plasmid Plus kits? Visualizar 3213 - How much ethanol should be added to Buffer AP3/E (renamed to Buffer AW1) and Buffer AW (renamed to Buffer AW2) in the DNeasy Plant kit? Visualizar 3202 - Can GeneRead rRNA depletion kit be used to deplete rRNA from miRNA isolated using miRNeasy kit? Visualizar 3200 - What is the Maximum/Minimum amount of RNA that can be used with GeneRead rRNA depletion kit? Visualizar 3201 - What quality of RNA can be used with the GeneRead rRNA depletion kit? Visualizar 3204 - How strict is the purification cut-off with GeneRead Size Selection kit? Visualizar 3203 - Is the RNeasy MinElute cleanup kit required for the GeneRead rRNA depletion kit? Visualizar 3205 - Can GeneRead Library preparation kits be used on species other than human mouse and rat, for example, plant species and microbial species? Visualizar 3206 - Can our GeneRead Library preparation kits be used with the SoLiD Platform? Visualizar 3248 - What is the difference between sponge, cubic and lamellar phase? Visualizar 3254 - When using the Nextal Cubic Phase products how do I know in which phase the sample is? What will I see? Visualizar 3255 - When using the NexTal Cubic Phase products, should I work with diluted or undiluted screens? Visualizar 3227 - In which solution can I keep my protein with the NexTal Cubic Phase kits? Visualizar 3225 - How pure should my protein be for the NexTal CubicPhase kits? Visualizar 3217 - How long can I store my purified RNA? Visualizar 3292-How do I start using the NeXtal CubicPhase kits? Visualizar 3295-What is the difference between SBTexcellerator HLA Core, Extended and Full Kits? Visualizar 3240 - What are the main differences between the qBiomarker PCR Arrays and the RT2 Profiler PCR Arrays? Visualizar 3237 - I would like to order the QuantiFast Probe Assays without master mix. Is this possible? Visualizar 3233 - Is the yield and quality of RNA and DNA purified with the AllPrep DNA/RNA FFPE Kit comparable to yield and quality of nucleic acids purified separately with other methods. Visualizar 3265 - Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)? Visualizar 3298-Will the sequence of the QuantiFast Probe Assays be provided? Visualizar 3262 - Which thermal cyclers are validated for use with the Investigator Nonaplex ESS Kit? Visualizar 3263 - Why are samples centrifuged at 4°C after the addition of chloroform when using the RNeasy Plus Universal Mini Kit? Visualizar 3241 - What are the recommended storage conditions of the QuantiFast Probe RT-PCR Plus Kit components? Visualizar 3264 - Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA? Visualizar 3238 - What are important points regarding the genomic DNA wipeout buffer in the QuantiFast Probe RT-PCR Plus Kit? Visualizar 3245 - What is the concentration of the primers and the probe in a reaction using the QuantiFast Probe Assays? Visualizar 3239 - What are the differences between the existing QuantiFast Probe RT-PCR Kit and the new Plus Kit? Visualizar 3247 - What is the correct matrix to use with the Investigator Nonaplex ESS Kit? Visualizar 3243 - What does the Investigator Nonaplex ESS Kit contain? Visualizar 3244 -What is the average length of a QuantiFast Probe Assay? Visualizar 3231 - Is there a size limit to the protein I can crystallize with the NexTal Cubic Phase products? Visualizar 3228 - Is it also possible to isolate miRNA with the AllPrep DNA/RNA FFPE Kit? Visualizar 3232 - Is the RNeasy Plus Universal Mini Kit suited for adipose, brain, and other fatty tissues? Visualizar 3229 - Is it necessary to perform calibration steps for the use of the MAX dye in duplex RT-PCR experiments on different cyclers? Visualizar 3236 - I use cultured cells as starting material. Can I also use the RNeasy Plus Universal Kit? Mostrar detalhes Is there also a miRNeasy Plus Universal Kit available? Visualizar 3261 - Which purification kits are recommended for the therascreen Pyro kits? Visualizar 3258 - Which filter set and settings should be used with the Investigator Nonaplex ESS Kit? Visualizar 3260 - Which purification kits are recommended for the Pyro kits? Visualizar 3259 - Which probe labels are available for the QuantiFast Probe Assays? Visualizar 3296-Why does the A peak reduction factor have to de set to 0.86 for the codon 61 assay in the therascreen NRAS Pyro Kit? Visualizar What plug-ins are available for the therascreen Pyro kits? FAQ ID - 3251 Visualizar 3249 - What is the optimal DNA volume and concentration for the Investigator Nonaplex ESS Kit? Visualizar What plug-ins are available for the Pyrosequencing kits? FAQ ID - 3250 Visualizar 3252 - What should be the concentration of my protein with the NexTal Cubic Phase products? Visualizar 3256 - Which crystallization solutions are compatible with lipidic cubic phase crystallization? Visualizar 3253 - What species are QuantiTect Probe Assays offered for? Visualizar 3257 - Which deparaffinization method can I use with the AllPrep DNA/RNA FFPE Kit? Visualizar Can the insertions in Exon 20 be detected with the therascreen EGFR Pyro Kit? Visualizar Can the insertions in Exon 20 be detected with the EGFR Pyro Kit? FAQ ID - 3279 Visualizar 3277 - Can I use the RNeasy Plus Universal Mini Kit for all types of tissue? Visualizar Can QuantiFast Probe Assays be ordered offline via e-mail or fax? FAQ ID - 3278 Visualizar 3283-Do you have any information or guidelines regarding the choice of reference genes for real-time PCR? Visualizar 3285-How are the genes in each iPSC qBiomarker PCR Array selected? Visualizar 3284-For which applications can the RNA isolated with the RNeasy Plus Universal Kit also be used? Visualizar Can the QuantiFast Probe Assay detect short fragmented RNA as well as longer fragments of RNA? Visualizar Do the RNeasy Plus Universal Mini/Midi kits contain both wash buffers, RW1 and RWT? FAQ ID - 3282 Visualizar 3270 - Are the primer sets different for the Investigator Nonaplex ESS Kit and ESSplex SE where the loci are the same? Visualizar Are the QuantiFast Probe Assays hydrolysis or FRET probes? FAQ ID - 3271 Visualizar Are panels and bin sets available for use with GeneMapper ID and GeneMapper ID-X? FAQ ID - 3267 Visualizar 3275 - Can I use more than 10ng DNA for the PCR reaction for my therascreen Pyro Kits? Visualizar Can I use more than 10ng DNA for the PCR reaction for the Pyro kits? FAQ ID - 3276 Visualizar 3274 - Can I do singleplex PCR with the QuantiFast Probe Assays? Visualizar 3273 - At what temperature can I perform the experiment with the NexTal Cubic Phase kits? Visualizar 3272 - Are the QuantiFast Probe Assays wet-lab validated? Visualizar 3222 - How much DNA/RNA can be isolated with the AllPrep DNA/RNA FFPE Kit? Visualizar 3218 - How many fragments are contained within the size standard BTO in the Investigator Nonaplex ESS Kit? Visualizar 3219 - How many freeze-thaw cycles can the master mix contained in the QuantiFast Probe RT-PCR Plus Kit tolerate? Visualizar 3220 - How many PCR cycles should I use for the Investigator Nonaplex ESS Kit? Visualizar 3226 - How pure should the chloroform used with the RNeasy Plus Universal Mini Kit be? Visualizar 3223 - How much sample material can I use for DNA/RNA purification with the AllPrep DNA/RNA FFPE Kit? Visualizar 3224 - How much time can be saved when using the RNeasy Mini QIAcube Kit? Visualizar 3289-How does the QuantiFast Probe RT-PCR Plus Kit eliminate genomic DNA contamination? Visualizar 3290-How do I analyze the data for the particular iPSC qBiomarker Arrays I have ordered? Visualizar 3288-How does the gDNA eliminator solution of the RNeasy Plus Universal Kit work? Visualizar 3294-How long can I store my purified DNA/RNA isolated with the AllPrep DNA/RNA FFPE kit? Visualizar 3286-How can I adjust the ROX concentration in the QuantiFast Probe RT-PCR Plus master mix? Visualizar 3291-How do I harvest the crystals from meso phase? Are special tools needed with the NexTal Cubic Phase products? Visualizar 3246 - What is the correct matrix to use with Investigator ESSplex SE Plus Kit? Visualizar 3297-Why does the PCR for HLA-DRB4 result in 2 amplicons of different size? Visualizar 3266 - Why are two different exon 2 forward sequencing primers used for the SBTexcellerator HLA-DRB1 Kit? Visualizar How can I find out if my gene of interest is expressed in a specific tissue type or cell line? FAQ ID - 3214 Visualizar 3215 - What is the starting material for SBTexcellerator HLA Kits? Visualizar Can I use genomic DNA from fixed samples with qBiomarker Copy Number PCR Arrays? FAQ ID — 3416 Visualizar Can I use amplified genomic DNA with qBiomarker Copy Number PCR Arrays? FAQ ID — 3415 Visualizar Do the samples require pretreatment before starting the manual protocol or before loading onto the QIAcube instrument? FAQ ID - 3479 Visualizar What are the expected yields of RNA from PAXgene Blood RNA Tubes isolated with the PAXgene Blood miRNA kit? FAQ ID - 3481 Visualizar Do you have stability data for RNA isolated with PAXgene miRNA Kit? FAQ ID - 3480 Visualizar Can the PAXgene Blood DNA Tubes be stored at 4°C? FAQ ID - 3493 Visualizar How should I thaw frozen PAXgene Blood DNA Tubes? FAQ ID - 3495 Visualizar Can the PAXgene Blood DNA Tube be frozen? If yes, at what temperatures and for how long? FAQ ID - 3491 Visualizar Can mitochondrial DNA be isolated with the PAXgene Blood DNA System? FAQ ID - 3498 Visualizar 3497 - What is the expected purity and size of DNA extracted using the PAXgene Blood DNA kit? FAQ ID - 3497 Visualizar Does the DNA extracted using the PAXgene Blood DNA System contain RNA? FAQ ID - 3499 Visualizar Can I multiplex the Microbial DNA qPCR Assays? FAQ ID — 3408 Visualizar Can I measure virulence factor gene expression? FAQ ID — 3405 Visualizar What sample types can I test on qBiomarker Copy Number PCR Arrays? FAQ ID — 3423 Visualizar What is the sensitivity for the Microbial DNA qPCR kits? FAQ ID — 3409 Visualizar Which probe labels are available for the Microbial DNA qPCR Assays? FAQ ID — 3407 Visualizar Are the Microbial DNA qPCR Assays wet-lab verified? FAQ ID — 3406 Visualizar Can I measure antibiotic resistance gene expression? FAQ ID — 3404 Visualizar What sequences are used to design the Microbial DNA qPCR Assays? FAQ ID — 3403 Visualizar Are the assays species-specific? FAQ ID — 3400 Visualizar Why is the copy number call lower than expected? FAQ ID — 3413 Visualizar Will qBiomarker Copy Number Assays work with heterogeneous samples like mixtures of tumor and normal tissue? FAQ ID — 3419 Visualizar Mostrar mais resultados Back to top Contact QIAGEN Global contacts Technical Service Customer Care