Product FAQ - QIAGEN



Product FAQ Bookmark Compartilhar more.. Você não está autorizado a baixar o recurso Organizar opções Sort alphabetically Sort by relevance Do I have to amplify the libraries generated with REPLI-g Single Cell RNA Library Kit to obtain full-length adapter sequences? FAQ ID - 3553 Visualizar What is the enzyme used in the whole transcriptome amplification step? FAQ ID - 3554 Visualizar Which cell collection methods are compatible with REPLI-g Single Cell RNA Library Kit? FAQ ID - 3555 Visualizar Should cells be washed before collection? FAQ ID - 3556 Visualizar What is the sample input volume for REPLI-g Single Cell RNA Library Kit? FAQ ID - 3557 Visualizar Are there special requirements for flow sorting? FAQ ID - 3558 Visualizar What kind of starting material can be used with the REPLI-g Single Cell RNA Library Kit? FAQ ID - 3548 Visualizar What is the maximum/minimum amount of DNA that can be used with REPLI-g Single Cell RNA Library Kit? FAQ ID - 3549 Visualizar What is the expected purity of DNA extracted from blood samples collected in PAXgene Blood DNA Tubes IVD? FAQ ID - 3511 Visualizar Is the quality and size of DNA extracted with the QIAamp DNA Mini and QIAamp Blood Mini kit good enough to generate DNA-libraries for next generation sequencing (NGS)? FAQ ID - 3518 Visualizar How much protein does one vial of Protease in the QIAamp spin kits contain? What is the concentration of the resuspended Protease? FAQ ID - 3520 Visualizar What is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue? FAQ ID - 3514 Visualizar Where can I find additional information for PreAnalytiX PAXgene products? FAQ ID - 3515 Visualizar How can samples be shipped and stored prior to extraction on the Autopure LS? FAQ ID - 3522 Visualizar How does the liquid level detection work and what is the minimal detectable volume for the conductive tips of the BioRobot Universal? FAQ ID - 3524 Visualizar How can samples be shipped and stored prior to extraction on the Autopure LS? FAQ ID - 3528 Visualizar What is the pipetting throughput of the BioRobot Universal? FAQ ID - 3527 Visualizar How do I obtain the concentration of my gDNA from the data analysis using the GeneRead DNA QuantiMIZE? FAQ ID - 3530 Visualizar What is the pipetting throughput of the BioRobot Universal? FAQ ID - 3525 Visualizar What specification do you have for the high-speed shaker system of the BioRobot Universal? FAQ ID - 3526 Visualizar Buffer SB1 in the GeneRead Size Selection Kit turned yellowish from a new kit. Can I still use it? FAQ ID - 3562 Visualizar Is it possible to use the REPLI-g WTA Single Cell kit to amplify miRNA? FAQ ID - 3563 Visualizar What is the sequence of the index in the GeneRead DNA Adapter I Set 1plex (catalog number 180912)? FAQ ID - 3561 Visualizar What is the accuracy of the high-speed dispensing system of the BioRobot Universal? FAQ ID - 3523 Visualizar Can I use the QuantiMIZE system for determination of gDNA quality for non-multiplex PCR enrichment methods (ex. Hybridization) (cat. No. NGQA-002, NGQC-100Y)? FAQ ID - 3531 Visualizar Which sample materials are CE-IVD validated for the RealStar Filovirus Screen? FAQ ID - 3529 Visualizar There is no barcode on the QIAsymphony cooling adapter, and the instrument asks for one. What do you suggest I do? FAQ ID - 3532 Visualizar Can I add bleach to the sample preparation waste? FAQ ID - 3505 Visualizar What is the recommended range of cells for the miScript Single Cell qPCR Kit? FAQ ID - 3565 Visualizar Can purified RNA be used with the miScript Single Cell qPCR Kit? FAQ ID - 3566 Visualizar Can the miScript Single Cell qPCR Kit be used to quantify piRNA expression? FAQ ID - 3567 Visualizar Can the miScript Single Cell qPCR Kit be used with plant cells? FAQ ID - 3568 Visualizar Are any of the miScript Single Cell qPCR Kit components interchangeable with miScript II RT Kit, miScript Plant RT Kit, miScript PreAMP PCR Kit, or miScript Microfluidics PreAMP Kit components? FAQ ID - 3569 Visualizar Should miScript PreAMP Primer Mixes be used in conjunction with the miScript Single Cell qPCR Kit? FAQ ID - 3570 Visualizar Is the cDNA cleanup step in the miScript Single Cell qPCR Kit workflow absolutely required? FAQ ID - 3571 Visualizar I have accidentally pipetted beads when removing the eluate after cDNA cleanup. What should I do? FAQ ID - 3572 Visualizar Does the miScript Single Cell qPCR Kit contain controls? FAQ ID - 3574 Visualizar When performing real-time PCR quality control associated with the miScript Single Cell qPCR Kit, is it necessary to perform control experiments for all individual samples/cells? FAQ ID - 3576 Visualizar I do not see any miRNA expression for my miRNAs of interest after performing single cell miRNA expression analysis with the miScript Single Cell qPCR Kit and miScript miRNA PCR Arrays or miScript Primer Assays. What could be the cause? FAQ ID - 3577 Visualizar What is the amplicon size of PCR products generated with the miScript Single Cell qPCR System? FAQ ID - 3578 Visualizar What is the dissociation curve temperature of PCR products generated with the miScript Single Cell qPCR System? FAQ ID - 3579 Visualizar When performing real-time PCR quality control associated with the miScript Single Cell qPCR Kit, is it necessary to perform control experiments for all individual samples/cells? FAQ ID - 3575 Visualizar Do you recommend normalizing single cell miRNA expression data to a “housekeeping” gene or miRNA? FAQ ID - 3580 Visualizar How should data be analyzed when the miScript Single Cell qPCR Kit is used with miScript miRNA PCR Arrays or miScript Primer Assays? FAQ ID - 3581 Visualizar What single cell isolation methods are recommended for use with the miScript Single Cell qPCR Kit? FAQ ID - 3582 Visualizar How would I provide information on the QuantiTect Primer assays in publications when the sequence is confidential? FAQ ID - 3593 Visualizar In which tubes is clot detection possible on QIAsymphony? FAQ ID - 3589 Visualizar How can I create a report/logfile on my QIAsymphony SP system? FAQ ID - 3594 Visualizar Is it possible to measure the proteolytic activity of proteins on the surface of exosomes that were isolated with ExoEasy? FAQ ID - 3599 Visualizar Which barcodes on sample tubes are accepted by the QIAsymphony SP? FAQ ID - 3598 Visualizar Can I change the language on my QIAsymphony SP system? FAQ ID - 3595 Visualizar Can I use blood, cells or bacteria with the QIAamp Fast DNA Tissue Kit? FAQ ID - 3587 Visualizar Can the Rotor-Gene Q be connected to LIMS? FAQ ID - 3596 Visualizar Can the REPLI-g Mitochondrial DNA Kit be used for species other than humans? FAQ ID - 3584 Visualizar Which Microbial qPCR Mastermix should I use? FAQ ID — 3395 Visualizar What are the storage conditions for the Microbial DNA qPCR products? FAQ ID — 3394 Visualizar 3327 - How often should the o-ring for the pipettor tip-adapter be changed on the QIAcube? Visualizar 3326 - Which QIAcube standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet? Visualizar 3328 - Can the Sample Tubes CB and the Sample Tubes RB be used interchangeably? Visualizar 3330 - Is there any contamination from E. coli genomic DNA in the polymerase provided with the Repli-G Single Cell Kit? Visualizar 3329 - When using REPLI-g, if the starting material is a single sorted cell, could mitochondrial DNA (mtDNA) be amplified? Visualizar 3331 - Should the REPLI-g amplified mtDNA be cleaned up before sequencing? Visualizar 3332 - Is there a method that can distinguish between non-amplified DNA and REPLI-g amplified DNA? Visualizar 3333 - When using REPLI-g, what is the purpose of the DTT added to the denaturation buffer in the cells/blood protocol? Visualizar What sample types can be tested on the arrays/assays? FAQ ID — 3399 Visualizar What are the minimum sample requirements for Microbial DNA qPCR kits? FAQ ID — 3393 Visualizar Can I use the Microbial DNA-Free Water and Microbial qPCR Mastermix if they have been opened more than 3 times? FAQ ID — 3398 Visualizar Visualizar 3353 - What is the composition of the elution buffer in the Ni-NTA Fast Start Kit? FAQ ID - 3353 Visualizar 3352 - What are the size ranges of Ni-NTA particles? FAQ ID - 3352 Visualizar 3354 - What type and amount of resin is packed into the Ni-NTA Spin columns from the Ni-NTA Spin Kit? FAQ ID - 3354 Visualizar 3341 - How are the EpiTect DNA controls produced and tested? FAQ - 3341 Visualizar 3351 - What is the upper limit for protein size that can bind to Ni-NTA agarose resin? FAQ ID - 3351 Visualizar 3344 - What is the difference between the EpiTect Plus DNA Bisulfite kit compared to the classic kit? FAQ - 3344 Visualizar 3345 - What is the difference between the high and low concentration EpiTect Bisulfite Conversion protocol? FAQ - 3345 Visualizar 3311 - Can QuickLyse miniprep kits be used with QIAVac 24 Plus? Visualizar 3313 - What is the endotoxin level for the QIAGEN Plasmid Plus kits? Visualizar 3312 - For DirectPrep 96 miniprep, is it possible to use a half plate and save the other half for later prep? Visualizar 3314-What is the concentration of RNase A sold separately? Visualizar 3316-What is the concentration of the reconstituted QuantiFast Probe Assay? Visualizar Visualizar 3325 - What are the catalog numbers for the packing materials for the QIAcube? Visualizar 3324 - Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube? Visualizar 3335 - Can I use the DNA directly from enzymatic reaction for Epitect Bisulfite treatment with the Epitect Bisulfite kit (cat. no. 59104)? Visualizar 3336 - Can I use the linearized DNA for Epitect Bisulfite treatment with the Epitect Bisulfite kit? Visualizar 3339 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) negatively impact subsequent NGS sequencing? Visualizar 3337 - Which method is used for QIAGEN sequencing service? Visualizar 3338 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) have 3'-hydroxyl and 5'-phosphate groups? Visualizar 3343 - Why is it difficult to quantify DNA using UV/Vis spectroscopy and fluorescence methods following EpiTect Bisulfite Conversion? How best do you quantify it? FAQ - 3343 Visualizar 3342 - How is the unmethylated unconverted EpiTect DNA control to be used? FAQ - 3342 Visualizar 3315-What is the concentration of the reconstituted QuantiTect Primer Assay (200)? Visualizar 3322 - What is the concentration of the reconstituted miScript Primer Assay (100) and miScript Precursor Assay (100) tubes? Visualizar 3317 - Do you have a protocol for isolation of genomic DNA from cell cultures using the QIAamp cador Pathogen Mini Kit? Visualizar 3318 - What guidelines should I follow in designing primers for use with the EpiTect MSP Kit? Visualizar 3320 - What is the sensitivity of the Type-it HRM PCR Kit? Visualizar 3321 - What is the purpose of the C. elegans miR-39 spike-in control in the miRNeasy Serum/Plasma kit? Visualizar 3318 - What is the preferred amplicon size for performing PCR on DNA amplified with the EpiTect Whole Bisulfitome Kit? Visualizar 3305 - What is the minimal amount of starting material required for using the SBTexcellerator HLA Kits? Visualizar Visualizar Visualizar Visualizar 3306 - What is the optimal sample DNA concentration for the Investigator ESSplex SE Plus Kit? Visualizar 3304 - What is the maximum DNA input volume that can be used in the Investigator ESSplex SE Plus Kit? Visualizar 3310 - Which settings for the filter set Any5Dye must be adjusted before running the spectral calibration for Investigator ESSplex SE Plus Kit? Visualizar 3308 - Where can I find the template files for the Investigator ESSplex SE Plus Kit? Visualizar 3309 - Which filter set should be used with Investigator ESSplex SE Plus Kit? Visualizar What additional equipment is needed to purify RNA using the PAXgene 96 Blood RNA Kit? FAQ ID -499 Visualizar 3300 - Can isopropanol or methanol be used in place of ethanol in RNeasy kits? Visualizar 3301 - Do you have any information or guidelines regarding the choice of reference genes for real-time PCR? Visualizar 3302 - How long can I store my purified RNA? Visualizar 3210 - Can the 4-16K Centrifuge be used with a 208 V outlet? Visualizar 3211 - What is the maximum speed of the plate rotor 2 x 96 in the 4-16 or 4-16K? Visualizar 3212 - What is the minimum size of DNA fragment that can be isolated with QIAamp DNA Mini kit? Visualizar 3299-Can Buffer ER from the Endofree Plasmid kits be used instead of the Buffer ETR in QIAGEN Plasmid Plus kits? Visualizar 3213 - How much ethanol should be added to Buffer AP3/E (renamed to Buffer AW1) and Buffer AW (renamed to Buffer AW2) in the DNeasy Plant kit? Visualizar 3202 - Can GeneRead rRNA depletion kit be used to deplete rRNA from miRNA isolated using miRNeasy kit? Visualizar 3200 - What is the Maximum/Minimum amount of RNA that can be used with GeneRead rRNA depletion kit? Visualizar 3201 - What quality of RNA can be used with the GeneRead rRNA depletion kit? Visualizar 3204 - How strict is the purification cut-off with GeneRead Size Selection kit? Visualizar 3203 - Is the RNeasy MinElute cleanup kit required for the GeneRead rRNA depletion kit? Visualizar 3205 - Can GeneRead Library preparation kits be used on species other than human mouse and rat, for example, plant species and microbial species? Visualizar 3206 - Can our GeneRead Library preparation kits be used with the SoLiD Platform? Visualizar 3248 - What is the difference between sponge, cubic and lamellar phase? Visualizar 3254 - When using the Nextal Cubic Phase products how do I know in which phase the sample is? What will I see? Visualizar 3255 - When using the NexTal Cubic Phase products, should I work with diluted or undiluted screens? Visualizar 3227 - In which solution can I keep my protein with the NexTal Cubic Phase kits? Visualizar 3225 - How pure should my protein be for the NexTal CubicPhase kits? Visualizar 3217 - How long can I store my purified RNA? Visualizar 3292-How do I start using the NeXtal CubicPhase kits? Visualizar 3295-What is the difference between SBTexcellerator HLA Core, Extended and Full Kits? Visualizar 3240 - What are the main differences between the qBiomarker PCR Arrays and the RT2 Profiler PCR Arrays? Visualizar 3237 - I would like to order the QuantiFast Probe Assays without master mix. Is this possible? Visualizar 3233 - Is the yield and quality of RNA and DNA purified with the AllPrep DNA/RNA FFPE Kit comparable to yield and quality of nucleic acids purified separately with other methods. Visualizar 3265 - Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)? Visualizar 3298-Will the sequence of the QuantiFast Probe Assays be provided? Visualizar 3262 - Which thermal cyclers are validated for use with the Investigator Nonaplex ESS Kit? Visualizar 3263 - Why are samples centrifuged at 4°C after the addition of chloroform when using the RNeasy Plus Universal Mini Kit? Visualizar 3241 - What are the recommended storage conditions of the QuantiFast Probe RT-PCR Plus Kit components? Visualizar 3264 - Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA? Visualizar 3238 - What are important points regarding the genomic DNA wipeout buffer in the QuantiFast Probe RT-PCR Plus Kit? Visualizar Mostrar mais resultados Back to top Contact QIAGEN Global contacts Technical Service Customer Care