Product FAQ - QIAGEN



Product FAQ Bookmark Compartilhar more.. Você não está autorizado a baixar o recurso Organizar opções Sort alphabetically Sort by relevance The QIAsymphony RNA kit uses RLT plus as lysis buffer. Can samples lysed in RLT and then stored in the freezer be used on the QIAsymphony? FAQ ID - 3533 Visualizar Can smaller volumes of blood be processed from the PAXgene Blood DNA Tube? FAQ ID - 3500 Visualizar How should DNA purified using the PAXgene Blood DNA System be stored? FAQ ID - 3502 Visualizar Is it possible to interrupt the PAXgene Blood DNA kit protocol? FAQ ID - 3503 Visualizar Can I use the PAXgene Blood DNA Tube to collect blood from animals? FAQ ID - 3504 Visualizar Can PAXgene Blood DNA Tubes be stored at higher than room temperature (18–25°C)? FAQ ID - 3509 Visualizar What kind of starting material can be used with the REPLI-g Single Cell DNA Library Kit? FAQ ID - 3536 Visualizar What is the maximum/minimum amount of DNA that can be used with REPLI-g Single Cell DNA Library Kit? FAQ ID - 3537 Visualizar Does the REPLI-g Single Cell DNA Library Kit include adapters? FAQ ID - 3538 Visualizar Does the REPLI-g Single Cell DNA Library Kit include a size selection step? FAQ ID - 3539 Visualizar Is the REPLI-g Single Cell DNA Library Kit compatible with adapters and amplification primers from other suppliers? FAQ ID - 3540 Visualizar Do I have to amplify the libraries generated with REPLI-g Single Cell DNA Library Kit to obtain full-length adapter sequences? FAQ ID - 3541 Visualizar What is the enzyme used in the whole genome amplification step? FAQ ID - 3542 Visualizar Which cell collection methods are compatible with REPLI-g Single Cell DNA Library Kit? FAQ ID - 3543 Visualizar Should cells be washed before collection? FAQ ID - 3544 Visualizar What is the sample input volume for the REPLI-g Single Cell DNA Library Kit? FAQ ID - 3545 Visualizar Are there special requirements for flow sorting? FAQ ID - 3546 Visualizar Does the REPLI-g Single Cell RNA Library Kit include a size selection step? FAQ ID - 3551 Visualizar Does the REPLI-g Single Cell RNA Library Kit include adapters? FAQ ID - 3550 Visualizar Is the REPLI-g Single Cell RNA Library Kit compatible with adapters and amplification primers from other suppliers? FAQ ID - 3552 Visualizar Do I have to amplify the libraries generated with REPLI-g Single Cell RNA Library Kit to obtain full-length adapter sequences? FAQ ID - 3553 Visualizar What is the enzyme used in the whole transcriptome amplification step? FAQ ID - 3554 Visualizar Which cell collection methods are compatible with REPLI-g Single Cell RNA Library Kit? FAQ ID - 3555 Visualizar Should cells be washed before collection? FAQ ID - 3556 Visualizar What is the sample input volume for REPLI-g Single Cell RNA Library Kit? FAQ ID - 3557 Visualizar Are there special requirements for flow sorting? FAQ ID - 3558 Visualizar What kind of starting material can be used with the REPLI-g Single Cell RNA Library Kit? FAQ ID - 3548 Visualizar What is the maximum/minimum amount of DNA that can be used with REPLI-g Single Cell RNA Library Kit? FAQ ID - 3549 Visualizar What is the expected purity of DNA extracted from blood samples collected in PAXgene Blood DNA Tubes IVD? FAQ ID - 3511 Visualizar Is the quality and size of DNA extracted with the QIAamp DNA Mini and QIAamp Blood Mini kit good enough to generate DNA-libraries for next generation sequencing (NGS)? FAQ ID - 3518 Visualizar How much protein does one vial of Protease in the QIAamp spin kits contain? What is the concentration of the resuspended Protease? FAQ ID - 3520 Visualizar What is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue? FAQ ID - 3514 Visualizar Where can I find additional information for PreAnalytiX PAXgene products? FAQ ID - 3515 Visualizar How can samples be shipped and stored prior to extraction on the Autopure LS? FAQ ID - 3522 Visualizar How does the liquid level detection work and what is the minimal detectable volume for the conductive tips of the BioRobot Universal? FAQ ID - 3524 Visualizar How can samples be shipped and stored prior to extraction on the Autopure LS? FAQ ID - 3528 Visualizar What is the pipetting throughput of the BioRobot Universal? FAQ ID - 3527 Visualizar How do I obtain the concentration of my gDNA from the data analysis using the GeneRead DNA QuantiMIZE? FAQ ID - 3530 Visualizar What is the pipetting throughput of the BioRobot Universal? FAQ ID - 3525 Visualizar What specification do you have for the high-speed shaker system of the BioRobot Universal? FAQ ID - 3526 Visualizar Buffer SB1 in the GeneRead Size Selection Kit turned yellowish from a new kit. Can I still use it? FAQ ID - 3562 Visualizar Is it possible to use the REPLI-g WTA Single Cell kit to amplify miRNA? FAQ ID - 3563 Visualizar What is the sequence of the index in the GeneRead DNA Adapter I Set 1plex (catalog number 180912)? FAQ ID - 3561 Visualizar What is the accuracy of the high-speed dispensing system of the BioRobot Universal? FAQ ID - 3523 Visualizar Can I use the QuantiMIZE system for determination of gDNA quality for non-multiplex PCR enrichment methods (ex. Hybridization) (cat. No. NGQA-002, NGQC-100Y)? FAQ ID - 3531 Visualizar Which sample materials are CE-IVD validated for the RealStar Filovirus Screen? FAQ ID - 3529 Visualizar There is no barcode on the QIAsymphony cooling adapter, and the instrument asks for one. What do you suggest I do? FAQ ID - 3532 Visualizar Can I add bleach to the sample preparation waste? FAQ ID - 3505 Visualizar What is the recommended range of cells for the miScript Single Cell qPCR Kit? FAQ ID - 3565 Visualizar Can purified RNA be used with the miScript Single Cell qPCR Kit? FAQ ID - 3566 Visualizar Can the miScript Single Cell qPCR Kit be used to quantify piRNA expression? FAQ ID - 3567 Visualizar Can the miScript Single Cell qPCR Kit be used with plant cells? FAQ ID - 3568 Visualizar Are any of the miScript Single Cell qPCR Kit components interchangeable with miScript II RT Kit, miScript Plant RT Kit, miScript PreAMP PCR Kit, or miScript Microfluidics PreAMP Kit components? FAQ ID - 3569 Visualizar Should miScript PreAMP Primer Mixes be used in conjunction with the miScript Single Cell qPCR Kit? FAQ ID - 3570 Visualizar Is the cDNA cleanup step in the miScript Single Cell qPCR Kit workflow absolutely required? FAQ ID - 3571 Visualizar I have accidentally pipetted beads when removing the eluate after cDNA cleanup. What should I do? FAQ ID - 3572 Visualizar Does the miScript Single Cell qPCR Kit contain controls? FAQ ID - 3574 Visualizar When performing real-time PCR quality control associated with the miScript Single Cell qPCR Kit, is it necessary to perform control experiments for all individual samples/cells? FAQ ID - 3576 Visualizar I do not see any miRNA expression for my miRNAs of interest after performing single cell miRNA expression analysis with the miScript Single Cell qPCR Kit and miScript miRNA PCR Arrays or miScript Primer Assays. What could be the cause? FAQ ID - 3577 Visualizar What is the amplicon size of PCR products generated with the miScript Single Cell qPCR System? FAQ ID - 3578 Visualizar What is the dissociation curve temperature of PCR products generated with the miScript Single Cell qPCR System? FAQ ID - 3579 Visualizar When performing real-time PCR quality control associated with the miScript Single Cell qPCR Kit, is it necessary to perform control experiments for all individual samples/cells? FAQ ID - 3575 Visualizar Do you recommend normalizing single cell miRNA expression data to a “housekeeping” gene or miRNA? FAQ ID - 3580 Visualizar How should data be analyzed when the miScript Single Cell qPCR Kit is used with miScript miRNA PCR Arrays or miScript Primer Assays? FAQ ID - 3581 Visualizar What single cell isolation methods are recommended for use with the miScript Single Cell qPCR Kit? FAQ ID - 3582 Visualizar How would I provide information on the QuantiTect Primer assays in publications when the sequence is confidential? FAQ ID - 3593 Visualizar In which tubes is clot detection possible on QIAsymphony? FAQ ID - 3589 Visualizar How can I create a report/logfile on my QIAsymphony SP system? FAQ ID - 3594 Visualizar Is it possible to measure the proteolytic activity of proteins on the surface of exosomes that were isolated with ExoEasy? FAQ ID - 3599 Visualizar Which barcodes on sample tubes are accepted by the QIAsymphony SP? FAQ ID - 3598 Visualizar Can I change the language on my QIAsymphony SP system? FAQ ID - 3595 Visualizar Can I use blood, cells or bacteria with the QIAamp Fast DNA Tissue Kit? FAQ ID - 3587 Visualizar Can the Rotor-Gene Q be connected to LIMS? FAQ ID - 3596 Visualizar Can the REPLI-g Mitochondrial DNA Kit be used for species other than humans? FAQ ID - 3584 Visualizar Which Microbial qPCR Mastermix should I use? FAQ ID — 3395 Visualizar What are the storage conditions for the Microbial DNA qPCR products? FAQ ID — 3394 Visualizar 3327 - How often should the o-ring for the pipettor tip-adapter be changed on the QIAcube? Visualizar 3326 - Which QIAcube standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet? Visualizar 3328 - Can the Sample Tubes CB and the Sample Tubes RB be used interchangeably? Visualizar 3330 - Is there any contamination from E. coli genomic DNA in the polymerase provided with the Repli-G Single Cell Kit? Visualizar 3329 - When using REPLI-g, if the starting material is a single sorted cell, could mitochondrial DNA (mtDNA) be amplified? Visualizar 3331 - Should the REPLI-g amplified mtDNA be cleaned up before sequencing? Visualizar 3332 - Is there a method that can distinguish between non-amplified DNA and REPLI-g amplified DNA? Visualizar 3333 - When using REPLI-g, what is the purpose of the DTT added to the denaturation buffer in the cells/blood protocol? Visualizar What sample types can be tested on the arrays/assays? FAQ ID — 3399 Visualizar What are the minimum sample requirements for Microbial DNA qPCR kits? FAQ ID — 3393 Visualizar Can I use the Microbial DNA-Free Water and Microbial qPCR Mastermix if they have been opened more than 3 times? FAQ ID — 3398 Visualizar Visualizar 3353 - What is the composition of the elution buffer in the Ni-NTA Fast Start Kit? FAQ ID - 3353 Visualizar 3352 - What are the size ranges of Ni-NTA particles? FAQ ID - 3352 Visualizar 3354 - What type and amount of resin is packed into the Ni-NTA Spin columns from the Ni-NTA Spin Kit? FAQ ID - 3354 Visualizar 3341 - How are the EpiTect DNA controls produced and tested? FAQ - 3341 Visualizar 3351 - What is the upper limit for protein size that can bind to Ni-NTA agarose resin? FAQ ID - 3351 Visualizar 3344 - What is the difference between the EpiTect Plus DNA Bisulfite kit compared to the classic kit? FAQ - 3344 Visualizar 3345 - What is the difference between the high and low concentration EpiTect Bisulfite Conversion protocol? FAQ - 3345 Visualizar 3311 - Can QuickLyse miniprep kits be used with QIAVac 24 Plus? Visualizar 3313 - What is the endotoxin level for the QIAGEN Plasmid Plus kits? Visualizar 3312 - For DirectPrep 96 miniprep, is it possible to use a half plate and save the other half for later prep? Visualizar 3314-What is the concentration of RNase A sold separately? Visualizar 3316-What is the concentration of the reconstituted QuantiFast Probe Assay? Visualizar Visualizar 3325 - What are the catalog numbers for the packing materials for the QIAcube? Visualizar 3324 - Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube? Visualizar 3335 - Can I use the DNA directly from enzymatic reaction for Epitect Bisulfite treatment with the Epitect Bisulfite kit (cat. no. 59104)? Visualizar 3336 - Can I use the linearized DNA for Epitect Bisulfite treatment with the Epitect Bisulfite kit? Visualizar 3339 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) negatively impact subsequent NGS sequencing? Visualizar 3337 - Which method is used for QIAGEN sequencing service? Visualizar 3338 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) have 3'-hydroxyl and 5'-phosphate groups? Visualizar 3343 - Why is it difficult to quantify DNA using UV/Vis spectroscopy and fluorescence methods following EpiTect Bisulfite Conversion? How best do you quantify it? FAQ - 3343 Visualizar 3342 - How is the unmethylated unconverted EpiTect DNA control to be used? FAQ - 3342 Visualizar 3315-What is the concentration of the reconstituted QuantiTect Primer Assay (200)? Visualizar 3322 - What is the concentration of the reconstituted miScript Primer Assay (100) and miScript Precursor Assay (100) tubes? Visualizar 3317 - Do you have a protocol for isolation of genomic DNA from cell cultures using the QIAamp cador Pathogen Mini Kit? Visualizar 3318 - What guidelines should I follow in designing primers for use with the EpiTect MSP Kit? Visualizar 3320 - What is the sensitivity of the Type-it HRM PCR Kit? Visualizar 3321 - What is the purpose of the C. elegans miR-39 spike-in control in the miRNeasy Serum/Plasma kit? Visualizar 3318 - What is the preferred amplicon size for performing PCR on DNA amplified with the EpiTect Whole Bisulfitome Kit? Visualizar 3305 - What is the minimal amount of starting material required for using the SBTexcellerator HLA Kits? Visualizar Visualizar Visualizar Mostrar mais resultados Back to top Contact QIAGEN Global contacts Technical Service Customer Care