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Is there something to consider when working with transduced cells treated with Polybrene?
3790
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For sequencing application, does the lysate require purification?
3792
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Are the CRISPR-Q PCR Assays and the CRISPR-Q Sanger Primers on GeneGlobe validated?
3793
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Are the PCR products generated with CRISPR-Q Custom PCR Assays only applicable to analysis of editing efficiency by Sanger sequencing?
3794
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Can the new CRISPR PCR Assays be used for dPCR on the QIAcuity? Do you have data or a protocol?
3795
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What is the minimum cell number needed for cell lysis?
3789
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For the Sanger analysis tool, the customer needs to upload two .abi1 files of forward and reverse sequences? Is there any additional info that needs to be provided?
3796
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What is the sequence of the QIAseq miRNA NGS 5’ Adapter?
FAQ ID - 3672
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What are recommended stopping points in the procedure of the EZ1&2 DNA FFPE Kits?
3753
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Are there other options for paraffin removal?
3754
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Can samples be lysed overnight with Proteinase K in the EZ1&2 DNA FFPE procedure?
3755
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Can the second Proteinase K lysis step in the EZ1&2 DNA FFPE procedure be carried out at lower temperatures than 65°C?
3756
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What is the difference between Buffer FTB and Buffer ATL?
3757
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Can the 2nd Proteinase K step be omitted?
3758
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What size of DNA can be expected?
3759
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Is the DNA extracted with the EZ1&2 DNA FFPE Kits suitable for downstream applications that require more intact DNA such as long-range PCR (>1 kb) and long-read DNA sequencing?
3760
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Do you recommend 1-step or 2-step real-time RT-PCR for gene expression analysis?
FAQ ID -1056
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3323 - Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube?
FAQ ID - 3323
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Does salt concentration in the food matter when using the DNeasy mericon Food Kit?
FAQ ID - 3346
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Why does the DNeasy mericon Food Kit use a QIAquick column and Buffer PB rather than a DNeasy column and Buffer AL, which might be expected since the kit isolates genomic DNA?
FAQ ID - 3347
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When would I use the mericon DNA Bacteria Kit vs the mericon DNA Bacteria Plus Kit?
FAQ ID - 3348
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Why is my 260/280 ratio low after using the DNeasy mericon Food Kit?
FAQ ID - 3349
Visualizar
CAn I use milk or other liquids with the DNeasy mericon Food kit? What volume would I use?
FAQ ID - 3350
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What is the composition of QIAzol? What is the color of this reagent?
FAQ ID - 3355
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Is it possible to stop the DNeasy tissue protocol and store the tissue lysates after digesting in buffer ATL and Proteinase K?
FAQ ID - 3362
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I cannot analyze the data from your Investigator HID kits with my GeneMapper software. What can I do?
FAQ ID - 3363
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What is the QIAxcel QX RNA Size Marker 200-6000nt?
FAQ ID - 3365
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What is the nature of the gel in the QIAxcel RNA QC kit v2.0? Is it denaturing?
FAQ ID - 3366
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Do you sell CoralLoad dye separately?
FAQ ID - 3368
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Are QIAcube reagent bottles (cat. no. 990393) autoclavable? What type of plastic are they made of?
FAQ ID - 3369
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What is the composition of elution buffers used in QIAsymphony DNA Investigator kits?
FAQ ID - 3387
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Can I buy the RNeasy Mini columns, RNeasy MinElute columns, RNeasy Midi columns or the RNeasy Maxi columns separately?
FAQ ID - 3388
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Can I buy the gDNA eliminator plates supplied in your RNeasy Plus 96 kit separately?
FAQ ID - 3389
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Can I buy the gDNA eliminator columns supplied in your RNeasy Plus kits separately?
FAQ ID - 3390
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Why does AllPrep DNA/RNA/Protein Mini kit use buffer RLT, but Allprep DNA/RNA Mini and Allprep DNA/RNA Micro kits use buffer RLT Plus? Are these buffers interchangeable?
FAQ ID - 3391
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Do you have data to show lowest dropout rate with Repli-G WTA Single Cells kit?
FAQ ID - 3392
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What is the RNase A concentration and composition of Buffer P1?
FAQ ID -198
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Are protocols also available for other instruments (e.g., Hamilton Star and Tecan Fluent)?
3797
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Where can I download the protocol for the KingFisher Flex?
3798
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Who can help in case of issues with the third party instrument?
3799
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Is the virus inactivated during the procedure?
3800
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Can I use sample volumes other than 300 µl?
3801
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Who can help if it is not clear if issues are caused by the third party instrument or by the chemistry?
3802
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I would like to use the kit on another third party instrument. Who should I contact?
3803
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How long does a saliva collection take?
3804
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What can I do to stimulate saliva flow?
3805
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Can I stop saliva collection before the fill line is reached?
3806
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What can I do if I don't see the fill line anymore due to saliva bubbles?
3807
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What happens if I accidentally unscrew the funnel before saliva collection is completed?
3808
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May I continue with the saliva collection if I accidentally unscrewed the funnel prematurely?
3809
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What is in the additive in the PAXgene Saliva Collector?
3810
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What is the shelf life of unused PAXgene Saliva Collectors?
3811
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How should unused PAXgene Saliva Collector be stored?
3812
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What should I avoid before sample collection?
3813
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How does the stabilizing reagent in the PAXgene Saliva Collector stabilize saliva?
3814
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How long can saliva in PAXgene Saliva Collector tubes be stored at room temperature?
3815
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Can saliva in PAXgene Saliva Collector tubes be stored at temperatures higher than 25°C?
3816
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Can saliva in PAXgene Saliva Collector tubes be stored frozen at –20 or –80°C?
3817
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Is RNA preserved in saliva collected in PAXgene Saliva Collector?
3818
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Are pathogens inactivated in human saliva samples collected with the PAXgene Saliva Collector?
3819
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What should I do if stabilized saliva samples are very heterogenous?
3820
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What is the expected DNA purity from saliva collected and stabilized with the PAXgene Saliva Collector?
3823
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Is it possible to process volumes of saliva from the PAXgene Saliva Collector other than the 400 or 1000 µl from the standard protocols for the QIAsymphony DNA Midi Kit using the QIAsymphony SP instrument?
3824
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Is it possible to purify DNA from the whole sample of a PAXgene Saliva Collector in one preparation?
3826
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Which downstream applications have been tested with DNA purified from saliva collected into PAXgene Saliva Collector?
3827
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Which downstream applications have been tested with SARS-CoV-2-derived RNA purified from saliva collected into PAXgene Saliva Collector?
3828
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Is a restriction digest necessary for DNA isolated from PAXgene Saliva samples before performing a dPCR run using the QIAcuity?
3829
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Is it possible to place the PAXgene Saliva Collector tube directly on the QIAsymphony SP instrument sample rack?
3825
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What is the expected DNA yield per milliliter saliva collected and stabilized with the PAXgene Saliva Collector?
3822
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Which QIAGEN DNA extraction kits are compatible with the PAXgene Saliva Collector?
3821
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What are common sized libraries observed on a TapeStation, Bioanalyzer, or similar instruments?
FAQ ID - 3837
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Are the QIAseq miRNA NGS 5’ Adapter and QIAseq miRNA NGS RT Initiator compatible with the QIAseq miRNA UDI kits?
FAQ ID - 3836
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What is the sequence of the UDI 5' Adapter?
FAQ ID - 3835
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Can you perform qPCR analysis of miRNAs from libraries prepared with the QIAseq miRNA Library Kit?
FAQ ID - 3674
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What can be used to validate results obtained with the QIAseq miRNA Library Kit?
FAQ ID - 3675
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What is the maximum number of available sample indexes?
FAQ ID - 3665
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What are recommended stopping points during the QIAseq miRNA Library Kit procedure?
FAQ ID - 3663
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Should total RNA or small enriched RNA be used as the starting material for the QIAseq miRNA Library Kit?
FAQ ID - 3659
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What is the recommended read length for libraries prepared using the QIAseq miRNA Library Kit?
FAQ ID - 3668
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What is the acceptable number of reads per sample per miRNA sequencing run?
FAQ ID - 3673
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Do you have a protocol for the isolation of genomic DNA from whole blood for use in MRC-Holland MLPA® assays?
FAQ ID -1169
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Is training of lab personnel included with the purchase of a BioRobot EZ1?
FAQ ID -1239
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Do the foils sealing the EZ1 Reagent Cartridges have to be manually removed prior to loading the robot?
FAQ ID -1240
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How can we get our BioRobot EZ1 upgraded when new protocols are launched?
FAQ ID -1241
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Where can I find information about the worktable setup on the BioRobot EZ1?
FAQ ID -1243
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What disposables are required for nucleic acid isolations on the BioRobot EZ1?
FAQ ID -1246
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How should I prepare buffy coat samples for use on the BioRobot EZ1?
FAQ ID -1249
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Do you have a protocol for the isolation of DNA from buffy coat using the BioRobot EZ1?
FAQ ID -985
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What has to be done to an RNA sample before loading it onto an Agilent Bioanalyzer?
FAQ ID -528
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Do you have a protocol for the isolation of total nucleic acids from animal and human tissues on the BioRobot EZ1?
FAQ ID -975
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Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the BioRobot EZ1?
FAQ ID -976
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Do you have a protocol for the isolation of DNA from fungi using the BioRobot EZ1?
FAQ ID -992
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Are the new EZ1 RNA Kits (cat. no. 958034; 959034; 956034) compatible with the previous EZ1 RNA Card (cat. no. 9015590) or EZ1 RNA Universal Tissue Card (cat. no 9016384)?
FAQ ID -1025
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What are the advantages of EnzScript M-MLV Reverse Transcriptase RNase H-(P7600) versus wild type M-MLV Reverse Transcriptase (P7040)?
FAQ ID - 3838
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What types of priming are compatible with EnzScript M-MLV Reverse Transcriptase RNase-H-?
FAQ ID - 3839
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What is the optimal reaction temperature for EnzScript M-MLV Reverse Transcriptase RNase H-?
FAQ ID - 3840
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What PCR enzymes can be used following the first-strand synthesis?
FAQ ID - 3841
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What is the suggested protocol for generating long, full-length cDNA transcripts (>5 kb)?
FAQ ID - 3842
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What region of LINE gets targeted by the assay for PyroMark Q96 MD or PyroMark Q24?
2857
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Does the PyroMark LINE assay target all LINE sequences?
2860
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What is the amplicon length of the PyroMark CpG LINE assay?
2856
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How do I perform the PyroMark Q24 Advanced upgrade?
FAQ ID -3163
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Is it possible to use PyroMark Q24 Advanced reagents on PyroMark Q24 system?
FAQ ID -3160
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What are the differences between new PyroMark Q24 Advanced reagents chemistry and PyroMark Q24 Gold reagents?
FAQ ID -3165
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How accurate and reliable is PyroMark Q24 in mutation analysis?
FAQ ID -2094
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We recently changed the OS from Windows XP to Windows 7. When re-installing software Pyromark Q24 2.0.6, it fails to analyze the results. Any suggestions?
FAQ ID - 3621
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What is the use of the PyroMark Q24 Validation Oligo?
FAQ ID -2855
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How does the built-in QC control for complete bisulfite conversion of DNA work on PyroMark Q24?
FAQ ID -2095
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How long does a single run on the PyroMark Q24 take?
FAQ ID -2096
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3843-How-do-I-prevent-a-drifting-baseline-in-my-pyrosequencing-pyrogram
FAQ ID - 3843
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When do I have to change the pulse settings/methods in a pyrosequencing run setup?
FAQ ID -2942
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What is a PyroMark instrument method or instrument code?
FAQ ID -2941
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Can the PyroMark Q96 CpG LINE assay be used with an ID system?
2861
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How many times can the cartridges for PyroMark Q24 or PyroMark Q96 ID instruments be reused?
FAQ ID -2863
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How do I prevent a drifting baseline in my pyrosequencing pyrogram? If this method cannot be selected automatically in the application software, you can download the method/code file from the instrument webpage.
FAQ ID -2878
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Can unused wells in a pyrosequencing plate be used in the next run?
FAQ ID -2872
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What is the reason for signals ceasing in the middle of a pyrosequencing run?
FAQ ID -2875
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Is the CpG software included in the PyroMark instruments to study methylation status?
FAQ ID -2842
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What is the reason for split peaks appearing in between dispensations on my pyrosequencing pyrogram?
FAQ ID -2881
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Where can I find explanations to the warning given by the PyroMark software after run data analysis?
FAQ ID -2874
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Which heating block is recommended for the pyrosequencing annealing step?
FAQ ID -2838
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How many times can vacuum troughs be reused with the PyroMark Vacuum Preparation Stations?
FAQ ID -2848
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What kind of shaker should be used for the pyrosequencing binding step?
FAQ ID -2837
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What is the reason for a high substrate peak in the pyrosequencing pyrogram?
FAQ ID -2879
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How many CpG sites are analyzed by the PyroMark CpG LINE assay?
2858
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Is there a user manual available for the PyroMark Assay design software?
FAQ ID -2851
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Which operating system is compatible with PyroMark IdentiFire Software?
FAQ ID - 3340
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How do I set up a PyroMark CpG Assay?
FAQ ID -2814
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What is the purpose of the unmethylated and unconverted control DNA of the EpiTect PCR Control DNA Set?
FAQ ID -2007
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Will the primer sequence for the PyroMark CpG Assay be provided?
FAQ ID -2823
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Does QIAGEN offer a design of Custom PyroMark CpG Assays?
FAQ ID -2818
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How are the PyroMark CpG Assays reconstituted?
FAQ ID -2817
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How are the PyroMark CpG Assays shipped and stored?
FAQ ID -2816
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What are the features of PyroMark CpG Assays, for example, in terms of design and validation?
FAQ ID -2821
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What is included in a PyroMark Custom Assay?
FAQ ID -2815
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In which format can PyroMark CpG Assays be ordered?
FAQ ID -2824
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Which kits can be used in combination with the PyroMark CpG Assays and PyroMark Custom Assays?
FAQ ID -2822
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Does the PyroMark Assay Design or application software give any cycling conditions for individual assay primers or a PCR setup pipetting scheme?
FAQ ID -2862
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What is the recommended amplicon size for CpG assays?
FAQ ID -2825
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What kind of reading length can I expect when using pyrosequencing technology for sequence analysis?
FAQ ID -2216
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Will dUTP in a PCR reaction affect pyrosequencing?
FAQ ID -2843
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Which purity grade is recommended for pyrosequencing primers?
FAQ ID -2832
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What concentration should be used for the sequencing primer in pyrosequencing?
FAQ ID -2826
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What is the concentration of PyroMark Control Oligo?
FAQ ID -2846
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What is the sample throughput of pyrosequencing systems?
FAQ ID -2215
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How do I reduce background peaks in the pyrosequencing pyrogram?
FAQ ID -2877
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What is the sensitivity limitation for pyrosequencing?
FAQ ID -2840
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What should be the single peak height for the PyroMark Control Oligo on the different PyroMark instruments?
FAQ ID -2852
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Which end of the PCR primer for pyrosequencing should be biotinylated?
FAQ ID -2839
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How many nucleotides of a homopolymer can be resolved in pyrosequencing?
FAQ ID -2871
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Where can I order the Streptavidin Sepharose beads for pyrosequencing?
FAQ ID -2850
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What are critical steps during CTC enrichment?
3830
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How pure are the CTCs enriched from blood samples using the AdnaTest?
3831
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Can frozen blood be used for the procedure?
3832
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Can the EZ2 AdnaTest be used for other body fluids than blood?
3833
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Can Heparin blood be used?
3834
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Can the kit be used on the EZ2 Connect?
FAQ ID - 3845
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Can I use a different elution buffer?
FAQ ID - 3847
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What is the average amount of cfDNA present in 1 ml plasma?
FAQ ID - 3846
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Can the kit be used on the EZ1 Advanced XL?
FAQ ID - 3844
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Do you have a protocol for purification of cytoplasmic RNA from animal cells?
FAQ ID -1257
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What kits does QIAGEN offer to extract RNA from whole blood?
FAQ ID -304
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What is the composition of Buffer RLT?
FAQ ID -2793
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What is the key technical challenge in isolating high quality RNA from cell or tissue samples?
FAQ ID -2656
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Do you have a protocol for the isolation of RNA from leukocytes in milk?
FAQ ID -952
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What is the maximum binding capacity of RNeasy spin columns?
FAQ ID -290
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Can QIAquick Kits be used to clean up RNA samples?
FAQ ID -490
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Are RNeasy spin columns sold separately?
FAQ ID -159
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Can the RNase-Free DNase Set be used for DNase digestions of RNA in solution?
FAQ ID -619
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Which kit should be used to extract RNA from adipose tissue, brain, and other fatty animal tissues?
FAQ ID -467
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How can I minimize degradation of RNA from my pancreas sample?
FAQ ID -624
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What is the composition of Buffer RW1?
FAQ ID -2796
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Do you have a protocol for purification of total DNA from crude lysates?
FAQ ID -1255
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Do you have a protocol for isolation of genomic DNA from saliva and mouthwash?
FAQ ID -917
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Do you have a protocol for purification of total DNA from yeast?
FAQ ID -1253
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Do you have a protocol for the isolation of DNA from soft tissues using the TissueLyser?
FAQ ID -924
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Do you have a protocol for purification of total DNA from insects?
FAQ ID -1254
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What is the shelf-life for QIAGEN Proteinase K (cat. no. 19131, 19133)?
FAQ ID - 3447
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Do you have a protocol for the isolation of genomic DNA from sperm?
FAQ ID -909
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What is the advantage of running an analytical gel with fractions of my plasmid preparation?
FAQ ID -769
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What is the recipe for LB?
FAQ ID -212
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How do I know if my plasmid is a high- or low copy number type?
FAQ ID -350
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Can I eliminate RNase A from buffer P1 for my plasmid preparation to obtain RNase-free DNA for in-vitro transcription?
FAQ ID -366
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I left Buffer P1 at room temperature after addition of RNase A, what shall I do?
FAQ ID -859
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With LyseBlue reagent for lysis control, can I now process more bacterial culture and overload the columns?
FAQ ID -864
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What is the recipe for 2x YT?
FAQ ID -213
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What are the additional plasmid bands I see on my gel?
FAQ ID -1059
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Do you have a protocol for the isolation of plasmid DNA from Bacillus subtilis?
FAQ ID -896
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What is the composition of Buffer PB?
FAQ ID -2791
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Do you have a protocol for obtaining > 20 µg plasmid DNA using the QIAprep Spin Miniprep Kit?
FAQ ID -3102
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Why is my plasmid DNA yield low?
FAQ ID -768
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Why do I get genomic DNA contamination in my plasmid prep?
FAQ ID -353
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Do you have a protocol for the isolation of plasmid DNA from Agrobacterium?
FAQ ID -898
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I am seeing a precipitate after adding LyseBlue reagent to Buffer P1. What should I do about that?
FAQ ID -1045
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What to do if cell clumps are present after Buffer P2 addition when using LyseBlue Reagent?
FAQ ID -861
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What is the recipe for SOC medium?
FAQ ID -798
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How much RNA does a typical mammalian cell contain?
FAQ ID -2946
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Do I have to discard Buffer RLT with beta-Mercaptoethanol (ß-ME) added to it after 1 month of storage?
FAQ ID -1037
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What are the effects of low A260/A230 ratios in RNA preparations on downstream applications?
FAQ ID -2248
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Do I need to purchase any consumable for processing the kit on EZ2 Connect?
FAQ ID - 3849
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Are we able to customize protocol on the EZ2 Connect?
FAQ ID - 3850
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Which version of the EZ2 Connect can run the EZ2 RNA/miRNA Tissue/Cells Kit?
FAQ ID - 3851
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Can the kit be run on EZ1 instruments?
FAQ ID - 3852
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How can I check the integrity of RNA purified using RNeasy Kits?
FAQ ID -1024
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What are the limits of detection of the QIAxcel Connect?
FAQ ID - 3853
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Are all QIAxcel Cartridge Kits compatible with QIAxcel Connect?
FAQ ID - 3856
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What are the differences between QIAxcel Advanced and QIAxcel Connect?
FAQ ID - 3857
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What is the QIAxcel Connect System?
FAQ ID - 3859
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Can I combine your CGT dPCR assays with custom designed assays?
FAQ ID - 3861
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How many assays can be multiplexed?
FAQ ID - 3862
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In which channels can you detect the CGT dPCR assays?
FAQ ID - 3863
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How can I resuspend the lyophilized assay?
FAQ ID - 3864
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What quenchers are used?
FAQ ID - 3865
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Do I need to use restriction enzymes when quantifying AAV genomes?
FAQ ID - 3866
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Can the CGT dPCR assays also be used for the quantification of non-AAV samples?
FAQ ID - 3868
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Can I use the CGT dPCR assays for the titer determination of RNA viruses?
FAQ ID - 3869
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How do I know if the assays are compatible with the sample DNA of interest?
FAQ ID - 3870
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Are the assays also compatible with qPCR?
FAQ ID - 3871
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Can they be used with lentiviral vectors?
FAQ ID - 3872
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Is restriction enzyme digestion required?
FAQ ID - 3875
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dPCR results are in copies per microliter. How do I calculate host cell DNA contamination in femtogram per microliter?
FAQ ID - 3876
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My samples are highly fragmented. Is the dPCR result for resDNA quantification accurate?
FAQ ID - 3877
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Is dPCR sensitive to inhibition?
FAQ ID - 3880
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Is DNA extraction from the samples needed?
FAQ ID - 3873
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How much sample can I load for detecting host cell contamination?
FAQ ID - 3879
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Where can I find a copy of the .kpf file for a BioSprint protocol?
FAQ ID -3073
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Can the DyeEx 96 kit be used to clean sequencing reactions to be run on ABI 3730?
FAQ ID -3075
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What is the minimum number of samples that can be extracted on a BioSprint 96?
FAQ ID -3076
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Can the FastLane Cell lysates (containing stabilized RNA, step 8. in Fastlane protocol) be stored?
FAQ ID -3077
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Can I use specific primers with the FastLane Kits?
FAQ ID -3078
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Can the FastLane RT reaction be incubated longer than 30 min at 42 deg C?
FAQ ID -3079
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Does carrier RNA interfere with the QuantiTect Whole Transcriptome amplification?
FAQ ID -3080
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Which SARS-CoV-2 targets can the SARS-CoV-2 Neo Kit detect?
FAQ ID - 3881
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Do we have specificity and sensitivity data for this kit?
FAQ ID - 3882
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Do we have specificity and sensitivity data for this kit?
FAQ ID - 3883
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Is it possible to scale up sample volume and reaction volume?
FAQ ID - 3884
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What color channels are used?
FAQ ID - 3885
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What is the percentage of false negative results that QIAGEN obtained during validations?
FAQ ID - 3886
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What is the minimum and maximum concentration of effective RNA for the test?
FAQ ID - 3887
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Anything important when starting the cycler run?
FAQ ID - 3888
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Is it possible to use ABI instruments without ROX as a passive reference?
FAQ ID - 3889
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What is the shelf-life of the Neo Assay Kit?
FAQ ID - 3890
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Can the SARS-CoV-2 Neo Assay Kit detect all variants?
FAQ ID - 3891
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Why did we not choose commonly used genes such as the S gene, E gene, or RdRP?
FAQ ID - 3892
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Why did you choose ORF1a+ORF1b and ORF3a+ORF7a?
FAQ ID - 3893
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Can I use ROX as passive reference dye with the SARS-CoV-2 Neo assay?
FAQ ID - 3894
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Can I use the IC RNA of the QIAprep& Viral RNA UM Kit?
FAQ ID - 3895
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Which positive control should I use?
FAQ ID - 3896
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How long can eluates be stored after sample preparation using the EZ1 instruments or EZ2 Connect MDx and the EZ1 DSP Virus Kit?
FAQ ID - 3897
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How stable is Phoenix Hot Start Taq when incubated in a PCR reaction mix at room temperature?
FAQ ID - 3898
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How can PCR cycling conditions be optimized for Phoenix Hot Start Taq?
FAQ ID - 3899
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Can Phoenix Hot Start Taq utilize cDNA as template for PCR?
FAQ ID - 3900
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Is Phoenix Hot Start Taq capable of multiplex PCR?
FAQ ID - 3901
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How can I optimize Mg2+ conditions for a specific amplicon when using Phoenix Hot Start Taq and the supplied reaction buffer?
FAQ ID - 3902
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When should I use Phoenix Hot Start Taq GC reaction Buffer?
FAQ ID - 3903
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What is the amplification length limit of Phoenix Hot Start Taq?
FAQ ID - 3904
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What is the fidelity/error rate of Phoenix Hot Start Taq?
FAQ ID - 3905
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Why is the Phoenix Hot Start Taq sometimes cloudy upon removing from -20°C storage?
FAQ ID - 3906
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How can yield for long targets be increased when using Phoenix Hot Start Taq?
FAQ ID - 3907
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How is VeraSeq 2.0 DNA Polymerase different from the standard recombinant Taq-B DNA Polymerase?
FAQ ID - 3908
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How can I optimize Mg2+ conditions for a specific amplicon when using VeraSeq 2.0 DNA Polymerase and the supplied reaction buffers?
FAQ ID - 3909
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When should I use 5X VeraSeq GC Buffer?
FAQ ID - 3910
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What is the amplification length limit of VeraSeq 2.0 DNA Polymerase?
FAQ ID - 3911
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Why is VeraSeq 2.0 DNA Polymerase sometimes cloudy upon removing from -20°C storage?
FAQ ID - 3913
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What is the amplification length limit of VeraSeq 2.0 DNA Polymerase?
FAQ ID - 3912
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How can yield for targets be increased when using VeraSeq 2.0 DNA Polymerase?
FAQ ID - 3914
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Will VeraSeq 2.0 DNA Polymerase incorporate dUTP?
FAQ ID - 3915
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Is VeraSeq 2.0 DNA Polymerase available as a hot start enzyme?
FAQ ID - 3916
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How long can reaction components incubate with VeraSeq 2.0 DNA Polymerase at room temperature prior to PCR cycling?
FAQ ID - 3917
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What denaturation temperature should be used in cycling conditions?
FAQ ID - 3918
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What annealing temperature should be used in the cycling conditions?
FAQ ID - 3919
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What is the stability of VeraSeq 2.0 DNA Polymerase at room temperature?
FAQ ID - 3920
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How can I optimize Mg2+ conditions for a specific amplicon when using VeraSeq ULtra DNA Polymerase and the supplied reaction buffers?
FAQ ID - 3922
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How is VeraSeq ULtra DNA Polymerase different from the standard recombinant Taq-B DNA Polymerase?
FAQ ID - 3921
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When should I use 5X VeraSeq GC Buffer?
FAQ ID - 3923
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What is the amplification length limit of VeraSeq ULtra DNA Polymerase?
FAQ ID - 3924
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What is the fidelity/error rate of VeraSeq ULtra DNA Polymerase?
FAQ ID - 3925
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How can yield for targets be increased when using VeraSeq ULtra DNA Polymerase?
FAQ ID - 3926
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Will VeraSeq ULtra DNA Polymerase incorporate dUTP?
FAQ ID - 3927
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Do the DNA fragments generated by VeraSeq ULtra DNA Polymerase have a single-base 3´ overhang?
FAQ ID - 3928
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Is VeraSeq ULtra DNA Polymerase available as a hot start enzyme?
FAQ ID - 3929
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What denaturation temperature should be used in the cycling conditions?
FAQ ID - 3931
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What annealing temperature should be used in the cycling conditions?
FAQ ID - 3932
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What are the ResDNA Quant Kit components?
FAQ ID - 3878
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How can I load new protocols onto the QIAcube?
FAQ ID -1421
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Are run reports and/or log files available on the QIAcube?
FAQ ID -1412
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What applications are offered on the QIAcube?
FAQ ID -1403
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Can the QIAcube heater/shaker be used independently from protocol runs?
FAQ ID -1422
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What EZ1 DNA Investigator protocol is recommended for very precious samples?
FAQ ID -1154
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When should the Normalization protocol be used?
FAQ ID -1155
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What fluorescent labels are 6-FAM, BTG, BTY, BTR, and BTO in Matrix Standard BT5 single or multi.cap?
FAQ ID - 3364
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How can I extract DNA from a polyacrylamide (PAGE) gel?
FAQ ID -120
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Why does my DNA sample float out of the slot when loading it onto an agarose gel?
FAQ ID -205
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What is the small band below my fragment of interest on an agarose gel after DNA cleanup using QIAquick?
FAQ ID -148
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I bound an 11 kb DNA fragment to a QIAquick column; is it completely lost?
FAQ ID -756
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Is it possible to isolate single stranded DNA (ssDNA) with the QIAEX II Kit from agarose or polyacrylamide gels?
FAQ ID -576
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Can DNA yields be increased by prolonging incubation with proteinase K in Buffer ATL?
FAQ ID -2032
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Can the QIAcube be connected to a laboratory information management system?
FAQ ID -1411
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What are the dimensions and weight of the QIAcube?
FAQ ID -1407
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What dedicated QIAcube Kits are available?
FAQ ID -2337
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How can I decontaminate the QIAcube system?
FAQ ID -1419
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Can the QIAcube rotor and/or buckets be removed for cleaning?
FAQ ID -1410
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Can the QIAcube centrifuge be used independently from protocol runs?
FAQ ID -1423
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Do I need to buy special kits for the QIAcube?
FAQ ID -1402
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Do I need to discard partially used QIAcube tip racks?
FAQ ID -1418
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Can I program my own protocols for the QIAcube?
FAQ ID -1405
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Can the CGT dPCR assays also be used for the analysis of AAV genome integrity?
FAQ ID - 3867
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Is the QIAprep& CRISPR Kit also applicable to other gene-editing technologies such as TALENs and ZFN?
3787
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Do you have a protocol for the isolation of viral RNA from stool?
FAQ ID -918
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Which extraction kits are recommended?
FAQ ID - 3874
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How long can I store the reconsituted assay regarding QIAcuity HEK293 resDNA Quant Kit (96) at 4°C?
FAQ ID - 3934
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What is the preferred storage temperature for reconstituted assay for the QIAcuity HEK293 resDNA Quant Kit (96)?
FAQ ID - 3935
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What is the amount of standard for the different kit?
FAQ ID - 3936
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How did you calculate the conversion factor?
FAQ ID - 3937
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What does the standard kit contain?
FAQ ID - 3938
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What region is targeted in the QIAcuity E. coli resDNA Quant Kit (96)?
FAQ ID - 3939
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How long are sample lysates stable in the fridge/ freezer?
FAQ ID -
143765
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Can solid samples be processed with the QIAsymphony DNA Investigator Kit on the instrument?
FAQ ID -2027
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Should sample lysate volumes be exactly 200 µL, 500 µL, or 1000 µL when loaded on the QIAsymphony SP?
FAQ ID -2031
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After bisulfite conversion, can the DNA be stored?
FAQ ID -2409
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Which version of the EZ2 Connect can run the EZ2 RNA FFPE Kit?
FAQ ID - 3940
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When should I use the standard vs. the fast protocol for the EZ2 RNA FFPE Kit?
FAQ ID - 3941
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What size of RNA can be expected?
FAQ ID - 3942
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Which version of the EZ2 Connect can run the EZ2 AllPrep DNA/RNA FFPE Kit?
FAQ ID - 3947
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When should I use the ‘Standard’ vs. the ‘Fast’ protocol for the EZ2 AllPrep DNA/RNA Kit
FAQ ID - 3948
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Are there other options for paraffin removal?
FAQ ID - 3949
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What are recommended stopping points in the procedure of the EZ2 AllPrep DNA/RNA FFPE Kit?
FAQ ID - 3950
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Why is there a need to overlay the resuspended DNA pellet with PRS again (step 17 of QSP)?
FAQ ID - 3951
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An immediate subsequent AllPrep DNA/RNA FFPE run does not start right away.
FAQ ID - 3952
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Can I use the EZ2 AllPrep DNA/RNA FFPE kit to extract DNA/RNA from fresh frozen tissue?
FAQ ID - 3953
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What size of RNA can be expected?
FAQ ID - 3954
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Are there specific recommendations for performing RT-PCR on RNA isolated from paraffin-embedded samples?
FAQ ID - 3955
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My overall RNA yield is fine but RT-PCR performance poor – how can I improve this?
FAQ ID - 3956
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Why are my DV200 values of my RNA sample so low?
FAQ ID - 3957
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The integrity of my DNA sample (e.g. QIAxcel Connect) is bad.
FAQ ID - 3958
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How do I proceed if the amount of starting material cannot be calculated in detail?
FAQ ID - 3943
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Are there other options for paraffin removal?
FAQ ID - 3946
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Are there specific recommendations for performing RT-PCR on RNA isolated from paraffin-embedded samples?
FAQ ID - 3944
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My overall RNA yield is fine but RT-PCR performance poor – how can I improve this?
FAQ ID - 3945
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Is this available without glycerol?
FAQ ID - 3959
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What types of priming are compatible with EnzScript M-MLV RT RNase H-?
FAQ ID - 3961
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What is the suggested protocol for generating long, full-length cDNA transcripts (> 5 kB)?
FAQ ID - 3964
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What are the advantages of EnzScript M-MLV Reverse Transcriptase RNase H- (P7600) versus wild type M-MLV Reverse Transcriptase (P7040)?
FAQ ID - 3960
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What PCR enzymes can be used following first-strand synthesis?
FAQ ID - 3963
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Is the kit compatible with unpurified in-process samples?
FAQ ID - 3965
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Do I need to use Proteinase K in the CGT Viral Vector Lysis Kit? What is the recommended concentration?
FAQ ID - 3971
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Do you recommend storage of highly diluted AAV samples?
FAQ ID - 3972
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Is it possible to store the lysates? How can the lysates be stored?
FAQ ID - 3973
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Can I use MspI or HpaII restriction enzymes from other suppliers?
FAQ ID - 3975
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How many AAV genome copies can I load into the QIAcuity?
FAQ ID - 3977
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Do you need to dilute the lysates before setting up the PCR mix?
FAQ ID - 3978
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How do I know if the CGT dPCR assays are compatible with the sample DNA of interest?
FAQ ID - 3980
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Are the CGT dPCR assays also compatible with qPCR?
FAQ ID - 3981
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Can the CGT Viral Vector Lysis kit be used with assays of other suppliers or custom designed assays?
FAQ ID - 3982
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What is included in the SureSilencing shRNA Plasmids shipment?
FAQ ID -2785
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Is the QIAxcel Advanced discontinued?
FAQ ID - 3983
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What is the concentration range for RNA Integrity Score analysis when using the QIAxcel RNA High Sensitivity Kit?
FAQ ID - 3984
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What effect does homogenization have on DNA yield and integrity when using QIAwave DNA/RNA Kits?
FAQ ID - 3985
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What water should I use to prepare the buffer concentrates?
FAQ ID - 3986
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What is the new Waste Tube made of?
FAQ ID - 3987
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Are the QIAwave kits based on a different chemistry than the legacy kits?
FAQ ID - 3990
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Is there a way to compare the environmental impacts of the kit?
FAQ ID - 3991
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Can the QIAwave kits be recycled?
FAQ ID - 3992
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Does the reuse of the Waste Tubes increase the risk of cross-contamination?
FAQ ID - 3988
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I don’t have any glassware in the lab, is the QIAwave kit still a good option for me?
FAQ ID - 3989
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Is the Reagent Bottle Rack, Grey, QC2 (cat. no. 9026197) compatible with the QIAcube Classic instrument?
FAQ ID - 3993
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Is the kit compatible with unpurified in-process samples?
FAQ ID - 3994
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My samples are expected to have low concentrations. Can I load more sample into the workflow or more lysate into the PCR reaction?
FAQ ID - 3995
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Can I use Internal Control as overall control?
FAQ ID - 3996
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How long can I store the reconstituted Internal Control and the reconstituted Positive Control?
FAQ ID - 3997
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Do you recommend storage of highly diluted mycoplasma samples?
FAQ ID - 3998
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What are the major differences between QIAseq Targeted RNA Panel TCR kit and previous QIAseq Immune Repertoire RNA library kit?
FAQ ID - 3999
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On which instrumentation will the RT² Profiler PCR Array work?
FAQ ID -2719
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Investigator Quantiplex: Which version of the Applied Biosystems 7500 Real-Time PCR System software can be used to run the Investigator Quantiplex?
FAQ ID -2569
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Investigator Quantiplex: Do I have to calibrate new dyes on my Applied Biosystems 7500 or 7500 Fast Real-Time PCR System?
FAQ ID -2571
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Which human target is used for the quantification in the Investigator Quantiplex Kit?
FAQ ID -2577
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Investigator Quantiplex Pro: On which real-time cyclers is the Investigator Quantiplex Pro Kit validated?
FAQ ID - 3728
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Investigator Quantiplex and Quantiplex Pro: What do I have to consider if I am using Applied Biosystems SDS software version 1.2.3?
FAQ ID -2570
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Investigator Quantiplex Pro: Which real-time cycler software version can be used to run the kit?
FAQ ID - 3730
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Investigator Quantiplex Pro RGQ: Which real-time cycler software version can be used to run the kit?
FAQ ID - 3743
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Investigator Quantiplex Pro RGQ: Which Rotor-Gene Q System can be used to run the kit?
FAQ ID - 3742
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Investigator Quantiplex Pro: Do I have to calibrate new dyes on my Applied Biosystems 7500 or QuantStudio 5 Real-Time PCR System?
FAQ ID - 3732
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How long does a run take?
FAQ ID -2566
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Investigator Quantiplex: For which real-time cyclers has the kit been validated?
FAQ ID -2567
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What are the storage conditions?
FAQ ID - 3748
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Investigator Quantiplex: Which version of the Rotor Gene-Q software can be used to run the kit?
FAQ ID -2568
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How can I streamline the quantification workflow?
FAQ ID - 3738
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Investigator Quantiplex Pro: Can the kit be run on the Rotor-Gene Q?
FAQ ID - 3729
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Investigator Quantiplex Pro RGQ: On which real-time cyclers is the kit validated?
FAQ ID - 3741
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Can I optimize a dPCR assay on the QIAcuity using gradient temperature?
FAQ ID - 3783
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Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with Rotor-Gene Probe Kits?
FAQ ID -2126
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Are probes other than TaqMan® probes, such as FRET probes, compatible with fast cycling with the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2130
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Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2131
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What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits?
FAQ ID -761
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Can I use REPLI-g for SNP Genotyping?
FAQ ID -700
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Can other probe technologies besides TaqMan® be used with the Type-it Fast SNP Probe PCR Kit?
FAQ ID -2058
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What downstream applications have been tested with DNA purified using the PAXgene Blood DNA System?
FAQ ID - 3506
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Can the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit be used with hybridization probes?
FAQ ID -2595
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Can I use the QuantiTect Multiplex PCR Kits on the Roche LightCycler systems with TaqMan® probes or QuantiTect Assays?
FAQ ID -717
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Can the QuantiFast Multiplex PCR Kits be used on Roche LightCycler systems with TaqMan® probes?
FAQ ID -1979
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Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with QuantiFast Probe PCR Kits?
FAQ ID -1452
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How is "Touchdown PCR" used to increase PCR specificity?
FAQ ID -75
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What should the starting template DNA quality and quantity be for PCR?
FAQ ID -74
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What kind of PCR products can be cloned with the QIAGEN PCR Cloning Kit?
FAQ ID -165
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Why do I get smeared PCR products?
FAQ ID -87
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Does QIAGEN sell Q-Solution separately?
FAQ ID -204
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Do CoralLoad dyes supplied in various QIAGEN PCR Kits interfere with downstream applications?
FAQ ID -1745
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Is Q-Solution required for PCR with QIAGEN's PCR kits?
FAQ ID -380
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Have you tested the effect of inhibitors on PCR performance?
FAQ ID -818
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What is the composition of the QIAGEN 10x PCR Buffer in Taq- and HotStarTaq DNA Polymerase Kits?
FAQ ID -606
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Do you have to use a restriction enzyme to relieve ITR secondary structures and increase target accessibility?
FAQ ID - 3974
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What is the recommended HpaII concentration?
FAQ ID - 3976
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Can I use the lysate from the CGT Viral Vector Lysis kit and combine it with another PCR system?
FAQ ID - 3979
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Do you have any recommendations on how to process my AAV samples?
FAQ ID - 3860
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What can be done if lower and upper alignment markers used with the QIAxcel System or QIAxcel Advanced are not aligned correctly?
FAQ ID -1834
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How do I safely inactivate biohazardous flow-through material?
FAQ ID -12
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How should I store plant material for DNA isolation using the DNeasy Plant Kit?
FAQ ID -114
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How can I precipitate genomic DNA using isopropanol?
FAQ ID -2953
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How do I perform a DNA precipitation to concentrate my sample?
FAQ ID -305
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What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?
FAQ ID -728
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What is the composition of buffer AE?
FAQ ID -730
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Do I need to have EDTA in the buffer in which I am going to store my isolated genomic DNA?
FAQ ID -754
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Do you have a protocol for the isolation of DNA from tofu?
FAQ ID -932
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Does the Epitect Bisulfite Kit also work on DNA extracted from plants?
FAQ ID -1209
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What QIAGEN kit can I use to isolate DNA from food products to test for genetically modified organisms (GMOs)?
FAQ ID -371
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