Product FAQ

 

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What can be used to validate results obtained with the QIAseq miRNA Library Kit?
FAQ ID - 3675
What is the maximum number of available sample indexes?
FAQ ID - 3665
What are recommended stopping points during the QIAseq miRNA Library Kit procedure?
FAQ ID - 3663
Should total RNA or small enriched RNA be used as the starting material for the QIAseq miRNA Library Kit?
FAQ ID - 3659
What is the recommended read length for libraries prepared using the QIAseq miRNA Library Kit?
FAQ ID - 3668
What is the acceptable number of reads per sample per miRNA sequencing run?
FAQ ID - 3673
Do you have a protocol for the isolation of genomic DNA from whole blood for use in MRC-Holland MLPA® assays?
FAQ ID -1169
Is training of lab personnel included with the purchase of a BioRobot EZ1?
FAQ ID -1239
Do the foils sealing the EZ1 Reagent Cartridges have to be manually removed prior to loading the robot?
FAQ ID -1240
How can we get our BioRobot EZ1 upgraded when new protocols are launched?
FAQ ID -1241
Where can I find information about the worktable setup on the BioRobot EZ1?
FAQ ID -1243
What disposables are required for nucleic acid isolations on the BioRobot EZ1?
FAQ ID -1246
How should I prepare buffy coat samples for use on the BioRobot EZ1?
FAQ ID -1249
Do you have a protocol for the isolation of DNA from buffy coat using the BioRobot EZ1?
FAQ ID -985
What has to be done to an RNA sample before loading it onto an Agilent Bioanalyzer?
FAQ ID -528
Do you have a protocol for the isolation of total nucleic acids from animal and human tissues on the BioRobot EZ1?
FAQ ID -975
Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the BioRobot EZ1?
FAQ ID -976
Do you have a protocol for the isolation of DNA from fungi using the BioRobot EZ1?
FAQ ID -992
Are the new EZ1 RNA Kits (cat. no. 958034; 959034; 956034) compatible with the previous EZ1 RNA Card (cat. no. 9015590) or EZ1 RNA Universal Tissue Card (cat. no 9016384)?
FAQ ID -1025
What are the advantages of EnzScript M-MLV Reverse Transcriptase RNase H-(P7600) versus wild type M-MLV Reverse Transcriptase (P7040)?
FAQ ID - 3838
What types of priming are compatible with EnzScript M-MLV Reverse Transcriptase RNase-H-?
FAQ ID - 3839
What is the optimal reaction temperature for EnzScript M-MLV Reverse Transcriptase RNase H-?
FAQ ID - 3840
What PCR enzymes can be used following the first-strand synthesis?
FAQ ID - 3841
What is the suggested protocol for generating long, full-length cDNA transcripts (>5 kb)?
FAQ ID - 3842
What region of LINE gets targeted by the assay for PyroMark Q96 MD or PyroMark Q24?
2857
Does the PyroMark LINE assay target all LINE sequences?
2860
What is the amplicon length of the PyroMark CpG LINE assay?
2856
How do I perform the PyroMark Q24 Advanced upgrade?
FAQ ID -3163
Is it possible to use PyroMark Q24 Advanced reagents on PyroMark Q24 system?
FAQ ID -3160
What are the differences between new PyroMark Q24 Advanced reagents chemistry and PyroMark Q24 Gold reagents?
FAQ ID -3165
How accurate and reliable is PyroMark Q24 in mutation analysis?
FAQ ID -2094
We recently changed the OS from Windows XP to Windows 7. When re-installing software Pyromark Q24 2.0.6, it fails to analyze the results. Any suggestions?
FAQ ID - 3621
What is the use of the PyroMark Q24 Validation Oligo?
FAQ ID -2855
How does the built-in QC control for complete bisulfite conversion of DNA work on PyroMark Q24?
FAQ ID -2095
How long does a single run on the PyroMark Q24 take?
FAQ ID -2096
3843-How-do-I-prevent-a-drifting-baseline-in-my-pyrosequencing-pyrogram
FAQ ID - 3843
When do I have to change the pulse settings/methods in a pyrosequencing run setup?
FAQ ID -2942
What is a PyroMark instrument method or instrument code?
FAQ ID -2941
Can the PyroMark Q96 CpG LINE assay be used with an ID system?
2861
How many times can the cartridges for PyroMark Q24 or PyroMark Q96 ID instruments be reused?
FAQ ID -2863
How do I prevent a drifting baseline in my pyrosequencing pyrogram? If this method cannot be selected automatically in the application software, you can download the method/code file from the instrument webpage.
FAQ ID -2878
Can unused wells in a pyrosequencing plate be used in the next run?
FAQ ID -2872
What is the reason for signals ceasing in the middle of a pyrosequencing run?
FAQ ID -2875
Is the CpG software included in the PyroMark instruments to study methylation status?
FAQ ID -2842
What is the reason for split peaks appearing in between dispensations on my pyrosequencing pyrogram?
FAQ ID -2881
Where can I find explanations to the warning given by the PyroMark software after run data analysis?
FAQ ID -2874
Which heating block is recommended for the pyrosequencing annealing step?
FAQ ID -2838
How many times can vacuum troughs be reused with the PyroMark Vacuum Preparation Stations?
FAQ ID -2848
What kind of shaker should be used for the pyrosequencing binding step?
FAQ ID -2837
What is the reason for a high substrate peak in the pyrosequencing pyrogram?
FAQ ID -2879
How many CpG sites are analyzed by the PyroMark CpG LINE assay?
2858
Is there a user manual available for the PyroMark Assay design software?
FAQ ID -2851
Which operating system is compatible with PyroMark IdentiFire Software?
FAQ ID - 3340
How do I set up a PyroMark CpG Assay?
FAQ ID -2814
What is the purpose of the unmethylated and unconverted control DNA of the EpiTect PCR Control DNA Set?
FAQ ID -2007
Will the primer sequence for the PyroMark CpG Assay be provided?
FAQ ID -2823
Does QIAGEN offer a design of Custom PyroMark CpG Assays?
FAQ ID -2818
How are the PyroMark CpG Assays reconstituted?
FAQ ID -2817
How are the PyroMark CpG Assays shipped and stored?
FAQ ID -2816
What are the features of PyroMark CpG Assays, for example, in terms of design and validation?
FAQ ID -2821
What is included in a PyroMark Custom Assay?
FAQ ID -2815
In which format can PyroMark CpG Assays be ordered?
FAQ ID -2824
Which kits can be used in combination with the PyroMark CpG Assays and PyroMark Custom Assays?
FAQ ID -2822
Does the PyroMark Assay Design or application software give any cycling conditions for individual assay primers or a PCR setup pipetting scheme?
FAQ ID -2862
What is the recommended amplicon size for CpG assays?
FAQ ID -2825
What kind of reading length can I expect when using pyrosequencing technology for sequence analysis?
FAQ ID -2216
Will dUTP in a PCR reaction affect pyrosequencing?
FAQ ID -2843
Which purity grade is recommended for pyrosequencing primers?
FAQ ID -2832
What concentration should be used for the sequencing primer in pyrosequencing?
FAQ ID -2826
What is the concentration of PyroMark Control Oligo?
FAQ ID -2846
What is the sample throughput of pyrosequencing systems?
FAQ ID -2215
How do I reduce background peaks in the pyrosequencing pyrogram?
FAQ ID -2877
What is the sensitivity limitation for pyrosequencing?
FAQ ID -2840
What should be the single peak height for the PyroMark Control Oligo on the different PyroMark instruments?
FAQ ID -2852
Which end of the PCR primer for pyrosequencing should be biotinylated?
FAQ ID -2839
How many nucleotides of a homopolymer can be resolved in pyrosequencing?
FAQ ID -2871
Where can I order the Streptavidin Sepharose beads for pyrosequencing?
FAQ ID -2850
What are critical steps during CTC enrichment?
3830
How pure are the CTCs enriched from blood samples using the AdnaTest?
3831
Can frozen blood be used for the procedure?
3832
Can the EZ2 AdnaTest be used for other body fluids than blood?
3833
Can Heparin blood be used?
3834
Can the kit be used on the EZ2 Connect?
FAQ ID - 3845
Can I use a different elution buffer?
FAQ ID - 3847
What is the average amount of cfDNA present in 1 ml plasma?
FAQ ID - 3846
Can the kit be used on the EZ1 Advanced XL?
FAQ ID - 3844
Do you have a protocol for purification of cytoplasmic RNA from animal cells?
FAQ ID -1257
What kits does QIAGEN offer to extract RNA from whole blood?
FAQ ID -304
What is the composition of Buffer RLT?
FAQ ID -2793
What is the key technical challenge in isolating high quality RNA from cell or tissue samples?
FAQ ID -2656
Do you have a protocol for the isolation of RNA from leukocytes in milk?
FAQ ID -952
What is the maximum binding capacity of RNeasy spin columns?
FAQ ID -290
Can QIAquick Kits be used to clean up RNA samples?
FAQ ID -490
Are RNeasy spin columns sold separately?
FAQ ID -159
Can the RNase-Free DNase Set be used for DNase digestions of RNA in solution?
FAQ ID -619
Which kit should be used to extract RNA from adipose tissue, brain, and other fatty animal tissues?
FAQ ID -467
How can I minimize degradation of RNA from my pancreas sample?
FAQ ID -624
What is the composition of Buffer RW1?
FAQ ID -2796
Do you have a protocol for purification of total DNA from crude lysates?
FAQ ID -1255
Do you have a protocol for isolation of genomic DNA from saliva and mouthwash?
FAQ ID -917
Do you have a protocol for purification of total DNA from yeast?
FAQ ID -1253
Do you have a protocol for the isolation of DNA from soft tissues using the TissueLyser?
FAQ ID -924
Do you have a protocol for purification of total DNA from insects?
FAQ ID -1254
What is the shelf-life for QIAGEN Proteinase K (cat. no. 19131, 19133)?
FAQ ID - 3447
Do you have a protocol for the isolation of genomic DNA from sperm?
FAQ ID -909
What is the advantage of running an analytical gel with fractions of my plasmid preparation?
FAQ ID -769
What is the recipe for LB?
FAQ ID -212
How do I know if my plasmid is a high- or low copy number type?
FAQ ID -350
Can I eliminate RNase A from buffer P1 for my plasmid preparation to obtain RNase-free DNA for in-vitro transcription?
FAQ ID -366
I left Buffer P1 at room temperature after addition of RNase A, what shall I do?
FAQ ID -859
With LyseBlue reagent for lysis control, can I now process more bacterial culture and overload the columns?
FAQ ID -864
What is the recipe for 2x YT?
FAQ ID -213
What are the additional plasmid bands I see on my gel?
FAQ ID -1059
Do you have a protocol for the isolation of plasmid DNA from Bacillus subtilis?
FAQ ID -896
What is the composition of Buffer PB?
FAQ ID -2791
Do you have a protocol for obtaining > 20 µg plasmid DNA using the QIAprep Spin Miniprep Kit?
FAQ ID -3102
Why is my plasmid DNA yield low?
FAQ ID -768
Why do I get genomic DNA contamination in my plasmid prep?
FAQ ID -353
Do you have a protocol for the isolation of plasmid DNA from Agrobacterium?
FAQ ID -898
I am seeing a precipitate after adding LyseBlue reagent to Buffer P1. What should I do about that?
FAQ ID -1045
What to do if cell clumps are present after Buffer P2 addition when using LyseBlue Reagent?
FAQ ID -861
What is the recipe for SOC medium?
FAQ ID -798
How much RNA does a typical mammalian cell contain?
FAQ ID -2946
Do I have to discard Buffer RLT with beta-Mercaptoethanol (ß-ME) added to it after 1 month of storage?
FAQ ID -1037
What are the effects of low A260/A230 ratios in RNA preparations on downstream applications?
FAQ ID -2248
Do I need to purchase any consumable for processing the kit on EZ2 Connect?
FAQ ID - 3849
Are we able to customize protocol on the EZ2 Connect?
FAQ ID - 3850
Which version of the EZ2 Connect can run the EZ2 RNA/miRNA Tissue/Cells Kit?
FAQ ID - 3851
Can the kit be run on EZ1 instruments?
FAQ ID - 3852
How can I check the integrity of RNA purified using RNeasy Kits?
FAQ ID -1024
What are the limits of detection of the QIAxcel Connect?
FAQ ID - 3853
Are all QIAxcel Cartridge Kits compatible with QIAxcel Connect?
FAQ ID - 3856
What are the differences between QIAxcel Advanced and QIAxcel Connect?
FAQ ID - 3857
What is the QIAxcel Connect System?
FAQ ID - 3859
Can I combine your CGT dPCR assays with custom designed assays?
FAQ ID - 3861
How many assays can be multiplexed?
FAQ ID - 3862
In which channels can you detect the CGT dPCR assays?
FAQ ID - 3863
How can I resuspend the lyophilized assay?
FAQ ID - 3864
What quenchers are used?
FAQ ID - 3865
Do I need to use restriction enzymes when quantifying AAV genomes?
FAQ ID - 3866
Can the CGT dPCR assays also be used for the quantification of non-AAV samples?
FAQ ID - 3868
Can I use the CGT dPCR assays for the titer determination of RNA viruses?
FAQ ID - 3869
How do I know if the assays are compatible with the sample DNA of interest?
FAQ ID - 3870
Are the assays also compatible with qPCR?
FAQ ID - 3871
Can they be used with lentiviral vectors?
FAQ ID - 3872
Is restriction enzyme digestion required?
FAQ ID - 3875
dPCR results are in copies per microliter. How do I calculate host cell DNA contamination in femtogram per microliter?
FAQ ID - 3876
My samples are highly fragmented. Is the dPCR result for resDNA quantification accurate?
FAQ ID - 3877
Is dPCR sensitive to inhibition?
FAQ ID - 3880
Is DNA extraction from the samples needed?
FAQ ID - 3873
How much sample can I load for detecting host cell contamination?
FAQ ID - 3879
Where can I find a copy of the .kpf file for a BioSprint protocol?
FAQ ID -3073
Can the DyeEx 96 kit be used to clean sequencing reactions to be run on ABI 3730?
FAQ ID -3075
What is the minimum number of samples that can be extracted on a BioSprint 96?
FAQ ID -3076
Can the FastLane Cell lysates (containing stabilized RNA, step 8. in Fastlane protocol) be stored?
FAQ ID -3077
Can I use specific primers with the FastLane Kits?
FAQ ID -3078
Can the FastLane RT reaction be incubated longer than 30 min at 42 deg C?
FAQ ID -3079
Does carrier RNA interfere with the QuantiTect Whole Transcriptome amplification?
FAQ ID -3080
Which SARS-CoV-2 targets can the SARS-CoV-2 Neo Kit detect?
FAQ ID - 3881
Do we have specificity and sensitivity data for this kit?
FAQ ID - 3882
Do we have specificity and sensitivity data for this kit?
FAQ ID - 3883
Is it possible to scale up sample volume and reaction volume?
FAQ ID - 3884
What color channels are used?
FAQ ID - 3885
What is the percentage of false negative results that QIAGEN obtained during validations?
FAQ ID - 3886
What is the minimum and maximum concentration of effective RNA for the test?
FAQ ID - 3887
Anything important when starting the cycler run?
FAQ ID - 3888
Is it possible to use ABI instruments without ROX as a passive reference?
FAQ ID - 3889
What is the shelf-life of the Neo Assay Kit?
FAQ ID - 3890
Can the SARS-CoV-2 Neo Assay Kit detect all variants?
FAQ ID - 3891
Why did we not choose commonly used genes such as the S gene, E gene, or RdRP?
FAQ ID - 3892
Why did you choose ORF1a+ORF1b and ORF3a+ORF7a?
FAQ ID - 3893
Can I use ROX as passive reference dye with the SARS-CoV-2 Neo assay?
FAQ ID - 3894
Can I use the IC RNA of the QIAprep& Viral RNA UM Kit?
FAQ ID - 3895
Which positive control should I use?
FAQ ID - 3896
How long can eluates be stored after sample preparation using the EZ1 instruments or EZ2 Connect MDx and the EZ1 DSP Virus Kit?
FAQ ID - 3897
How long can eluates be stored after sample preparation using the EZ1 instruments or EZ2 Connect MDx and the EZ1 DSP DNA Blood Kit?
FAQ ID -1569
Can I use sample preparation cartridges from other suppliers together with the EZ1 instruments or EZ2 Connect MDx?
FAQ ID -1567
How long can blood samples be stored before sample preparation using the EZ1 DSP DNA Blood system?
FAQ ID -1565
Which materials can be used as input samples for the QIAsymphony DSP Circulating DNA Kit?
FAQ ID - 3702
Are there important considerations for plasma generation and urine handling?
FAQ ID - 3699
How stable is Phoenix Hot Start Taq when incubated in a PCR reaction mix at room temperature?
FAQ ID - 3898
How can PCR cycling conditions be optimized for Phoenix Hot Start Taq?
FAQ ID - 3899
Can Phoenix Hot Start Taq utilize cDNA as template for PCR?
FAQ ID - 3900
Is Phoenix Hot Start Taq capable of multiplex PCR?
FAQ ID - 3901
How can I optimize Mg2+ conditions for a specific amplicon when using Phoenix Hot Start Taq and the supplied reaction buffer?
FAQ ID - 3902
When should I use Phoenix Hot Start Taq GC reaction Buffer?
FAQ ID - 3903
What is the amplification length limit of Phoenix Hot Start Taq?
FAQ ID - 3904
What is the fidelity/error rate of Phoenix Hot Start Taq?
FAQ ID - 3905
Why is the Phoenix Hot Start Taq sometimes cloudy upon removing from -20°C storage?
FAQ ID - 3906
How can yield for long targets be increased when using Phoenix Hot Start Taq?
FAQ ID - 3907
How is VeraSeq 2.0 DNA Polymerase different from the standard recombinant Taq-B DNA Polymerase?
FAQ ID - 3908
How can I optimize Mg2+ conditions for a specific amplicon when using VeraSeq 2.0 DNA Polymerase and the supplied reaction buffers?
FAQ ID - 3909
When should I use 5X VeraSeq GC Buffer?
FAQ ID - 3910
What is the amplification length limit of VeraSeq 2.0 DNA Polymerase?
FAQ ID - 3911
Why is VeraSeq 2.0 DNA Polymerase sometimes cloudy upon removing from -20°C storage?
FAQ ID - 3913
What is the amplification length limit of VeraSeq 2.0 DNA Polymerase?
FAQ ID - 3912
How can yield for targets be increased when using VeraSeq 2.0 DNA Polymerase?
FAQ ID - 3914
Will VeraSeq 2.0 DNA Polymerase incorporate dUTP?
FAQ ID - 3915
Is VeraSeq 2.0 DNA Polymerase available as a hot start enzyme?
FAQ ID - 3916
How long can reaction components incubate with VeraSeq 2.0 DNA Polymerase at room temperature prior to PCR cycling?
FAQ ID - 3917
What denaturation temperature should be used in cycling conditions?
FAQ ID - 3918
What annealing temperature should be used in the cycling conditions?
FAQ ID - 3919
What is the stability of VeraSeq 2.0 DNA Polymerase at room temperature?
FAQ ID - 3920
How can I optimize Mg2+ conditions for a specific amplicon when using VeraSeq ULtra DNA Polymerase and the supplied reaction buffers?
FAQ ID - 3922
How is VeraSeq ULtra DNA Polymerase different from the standard recombinant Taq-B DNA Polymerase?
FAQ ID - 3921
When should I use 5X VeraSeq GC Buffer?
FAQ ID - 3923
What is the amplification length limit of VeraSeq ULtra DNA Polymerase?
FAQ ID - 3924
What is the fidelity/error rate of VeraSeq ULtra DNA Polymerase?
FAQ ID - 3925
How can yield for targets be increased when using VeraSeq ULtra DNA Polymerase?
FAQ ID - 3926
Will VeraSeq ULtra DNA Polymerase incorporate dUTP?
FAQ ID - 3927
Do the DNA fragments generated by VeraSeq ULtra DNA Polymerase have a single-base 3´ overhang?
FAQ ID - 3928
Is VeraSeq ULtra DNA Polymerase available as a hot start enzyme?
FAQ ID - 3929
What denaturation temperature should be used in the cycling conditions?
FAQ ID - 3931
What annealing temperature should be used in the cycling conditions?
FAQ ID - 3932
What are the ResDNA Quant Kit components?
FAQ ID - 3878
How can I load new protocols onto the QIAcube?
FAQ ID -1421
Are run reports and/or log files available on the QIAcube?
FAQ ID -1412
What applications are offered on the QIAcube?
FAQ ID -1403
Can the QIAcube heater/shaker be used independently from protocol runs?
FAQ ID -1422
What EZ1 DNA Investigator protocol is recommended for very precious samples?
FAQ ID -1154
When should the Normalization protocol be used?
FAQ ID -1155
What fluorescent labels are 6-FAM, BTG, BTY, BTR, and BTO in Matrix Standard BT5 single or multi.cap?
FAQ ID - 3364
Does the EpiTect Hi-C Data Analysis Portal cost money?
FAQ ID - 143077
What is the effect of using amounts of input material per sample that fall outside the recommended range?
FAQ ID - 143064 
What is the required amount of input material per sample for the EpiTect Hi-C Kit?
FAQ ID - 143063
Does QIAGEN provide data analysis to accompany the EpiTect Hi-C Kit?
FAQ ID - 143075
How can I extract DNA from a polyacrylamide (PAGE) gel?
FAQ ID -120
Why does my DNA sample float out of the slot when loading it onto an agarose gel?
FAQ ID -205
What is the small band below my fragment of interest on an agarose gel after DNA cleanup using QIAquick?
FAQ ID -148
I bound an 11 kb DNA fragment to a QIAquick column; is it completely lost?
FAQ ID -756
Is it possible to isolate single stranded DNA (ssDNA) with the QIAEX II Kit from agarose or polyacrylamide gels?
FAQ ID -576
Can DNA yields be increased by prolonging incubation with proteinase K in Buffer ATL?
FAQ ID -2032
Can the QIAcube be connected to a laboratory information management system?
FAQ ID -1411
What are the dimensions and weight of the QIAcube?
FAQ ID -1407
What dedicated QIAcube Kits are available?
FAQ ID -2337
How can I decontaminate the QIAcube system?
FAQ ID -1419
Can the QIAcube rotor and/or buckets be removed for cleaning?
FAQ ID -1410
Can the QIAcube centrifuge be used independently from protocol runs?
FAQ ID -1423
Do I need to buy special kits for the QIAcube?
FAQ ID -1402
Do I need to discard partially used QIAcube tip racks?
FAQ ID -1418
Can I program my own protocols for the QIAcube?
FAQ ID -1405
Can the CGT dPCR assays also be used for the analysis of AAV genome integrity?
FAQ ID - 3867
Is the QIAprep&amp CRISPR Kit also applicable to other gene-editing technologies such as TALENs and ZFN?
3787
Do you have a protocol for the isolation of viral RNA from stool?
FAQ ID -918
Which extraction kits are recommended?
FAQ ID - 3874
How long can I store the reconsituted assay regarding QIAcuity HEK293 resDNA Quant Kit (96) at 4°C?
FAQ ID - 3934
What is the preferred storage temperature for reconstituted assay for the QIAcuity HEK293 resDNA Quant Kit (96)?
FAQ ID - 3935
What is the amount of standard for the different kit?
FAQ ID - 3936
How did you calculate the conversion factor?
FAQ ID - 3937
What does the standard kit contain?
FAQ ID - 3938
What region is targeted in the QIAcuity E. coli resDNA Quant Kit (96)?
FAQ ID - 3939
How long are sample lysates stable in the fridge/ freezer?
FAQ ID - 143765
Can solid samples be processed with the QIAsymphony DNA Investigator Kit on the instrument?
FAQ ID -2027
Should sample lysate volumes be exactly 200 µL, 500 µL, or 1000 µL when loaded on the QIAsymphony SP?
FAQ ID -2031
Which version of the EZ2 Connect can run the EZ2 RNA FFPE Kit?
FAQ ID - 3940
When should I use the standard vs. the fast protocol for the EZ2 RNA FFPE Kit?
FAQ ID - 3941
What size of RNA can be expected?
FAQ ID - 3942
Which version of the EZ2 Connect can run the EZ2 AllPrep DNA/RNA FFPE Kit?
FAQ ID - 3947
When should I use the ‘Standard’ vs. the ‘Fast’ protocol for the EZ2 AllPrep DNA/RNA Kit
FAQ ID - 3948
Are there other options for paraffin removal?
FAQ ID - 3949
What are recommended stopping points in the procedure of the EZ2 AllPrep DNA/RNA FFPE Kit?
FAQ ID - 3950
Why is there a need to overlay the resuspended DNA pellet with PRS again (step 17 of QSP)?
FAQ ID - 3951
An immediate subsequent AllPrep DNA/RNA FFPE run does not start right away.
FAQ ID - 3952
Can I use the EZ2 AllPrep DNA/RNA FFPE kit to extract DNA/RNA from fresh frozen tissue?
FAQ ID - 3953
What size of RNA can be expected?
FAQ ID - 3954
Are there specific recommendations for performing RT-PCR on RNA isolated from paraffin-embedded samples?
FAQ ID - 3955
My overall RNA yield is fine but RT-PCR performance poor – how can I improve this?
FAQ ID - 3956
Why are my DV200 values of my RNA sample so low?
FAQ ID - 3957
The integrity of my DNA sample (e.g. QIAxcel Connect) is bad.
FAQ ID - 3958
How do I proceed if the amount of starting material cannot be calculated in detail?
FAQ ID - 3943
Are there other options for paraffin removal?
FAQ ID - 3946
Are there specific recommendations for performing RT-PCR on RNA isolated from paraffin-embedded samples?
FAQ ID - 3944
My overall RNA yield is fine but RT-PCR performance poor – how can I improve this?
FAQ ID - 3945
Is this available without glycerol?
FAQ ID - 3959
What types of priming are compatible with EnzScript M-MLV RT RNase H-?
FAQ ID - 3961
What is the suggested protocol for generating long, full-length cDNA transcripts (> 5 kB)?
FAQ ID - 3964
What are the advantages of EnzScript M-MLV Reverse Transcriptase RNase H- (P7600) versus wild type M-MLV Reverse Transcriptase (P7040)?
FAQ ID - 3960
What PCR enzymes can be used following first-strand synthesis?
FAQ ID - 3963
Is the kit compatible with unpurified in-process samples?
FAQ ID - 3965
Do I need to use Proteinase K in the CGT Viral Vector Lysis Kit? What is the recommended concentration?
FAQ ID - 3971
Do you recommend storage of highly diluted AAV samples?
FAQ ID - 3972
Is it possible to store the lysates? How can the lysates be stored?
FAQ ID - 3973
Can I use MspI or HpaII restriction enzymes from other suppliers?
FAQ ID - 3975
How many AAV genome copies can I load into the QIAcuity?
FAQ ID - 3977
Do you need to dilute the lysates before setting up the PCR mix?
FAQ ID - 3978

How do I know if the CGT dPCR assays are compatible with the sample DNA of interest?
FAQ ID - 3980

Are the CGT dPCR assays also compatible with qPCR?
FAQ ID - 3981

Can the CGT Viral Vector Lysis kit be used with assays of other suppliers or custom designed assays?
FAQ ID - 3982

What is included in the SureSilencing shRNA Plasmids shipment?
FAQ ID -2785
Is the QIAxcel Advanced discontinued?
FAQ ID - 3983

What is the concentration range for RNA Integrity Score analysis when using the QIAxcel RNA High Sensitivity Kit?
FAQ ID - 3984

What effect does homogenization have on DNA yield and integrity when using QIAwave DNA/RNA Kits?
FAQ ID - 3985

What water should I use to prepare the buffer concentrates?
FAQ ID - 3986

What is the new Waste Tube made of?
FAQ ID - 3987

Are the QIAwave kits based on a different chemistry than the legacy kits?
FAQ ID - 3990

Is there a way to compare the environmental impacts of the kit?
FAQ ID - 3991

Can the QIAwave kits be recycled?
FAQ ID - 3992
Does the reuse of the Waste Tubes increase the risk of cross-contamination?
FAQ ID - 3988

I don’t have any glassware in the lab, is the QIAwave kit still a good option for me?
FAQ ID - 3989

Is the Reagent Bottle Rack, Grey, QC2 (cat. no. 9026197) compatible with the QIAcube Classic instrument?
FAQ ID - 3993
Is the kit compatible with unpurified in-process samples?
FAQ ID - 3994
My samples are expected to have low concentrations. Can I load more sample into the workflow or more lysate into the PCR reaction?
FAQ ID - 3995
Can I use Internal Control as overall control?
FAQ ID - 3996
How long can I store the reconstituted Internal Control and the reconstituted Positive Control?
FAQ ID - 3997
Do you recommend storage of highly diluted mycoplasma samples?
FAQ ID - 3998
What are the major differences between QIAseq Targeted RNA Panel TCR kit and previous QIAseq Immune Repertoire RNA library kit?
FAQ ID - 3999
On which instrumentation will the RT² Profiler PCR Array work?
FAQ ID -2719
Investigator Quantiplex: Which version of the Applied Biosystems 7500 Real-Time PCR System software can be used to run the Investigator Quantiplex?
FAQ ID -2569
Investigator Quantiplex: Do I have to calibrate new dyes on my Applied Biosystems 7500 or 7500 Fast Real-Time PCR System?
FAQ ID -2571
Which human target is used for the quantification in the Investigator Quantiplex Kit?
FAQ ID -2577
Investigator Quantiplex Pro: On which real-time cyclers is the Investigator Quantiplex Pro Kit validated?
FAQ ID - 3728
Investigator Quantiplex and Quantiplex Pro: What do I have to consider if I am using Applied Biosystems SDS software version 1.2.3?
FAQ ID -2570
Investigator Quantiplex Pro: Which real-time cycler software version can be used to run the kit?
FAQ ID - 3730
Investigator Quantiplex Pro RGQ: Which real-time cycler software version can be used to run the kit?
FAQ ID - 3743
Investigator Quantiplex Pro RGQ: Which Rotor-Gene Q System can be used to run the kit?
FAQ ID - 3742
Investigator Quantiplex Pro: Do I have to calibrate new dyes on my Applied Biosystems 7500 or QuantStudio 5 Real-Time PCR System?
FAQ ID - 3732
How long does a run take?
FAQ ID -2566
Investigator Quantiplex: For which real-time cyclers has the kit been validated?
FAQ ID -2567
What are the storage conditions?
FAQ ID - 3748
Investigator Quantiplex: Which version of the Rotor Gene-Q software can be used to run the kit?
FAQ ID -2568
How can I streamline the quantification workflow?
FAQ ID - 3738
Investigator Quantiplex Pro: Can the kit be run on the Rotor-Gene Q?
FAQ ID - 3729
Investigator Quantiplex Pro RGQ: On which real-time cyclers is the kit validated?
FAQ ID - 3741
Can I optimize a dPCR assay on the QIAcuity using gradient temperature?
FAQ ID - 3783
Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with Rotor-Gene Probe Kits?
FAQ ID -2126
Are probes other than TaqMan® probes, such as FRET probes, compatible with fast cycling with the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2130
Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2131
What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits?
FAQ ID -761
Can I use REPLI-g for SNP Genotyping?
FAQ ID -700
Can other probe technologies besides TaqMan® be used with the Type-it Fast SNP Probe PCR Kit?
FAQ ID -2058
What downstream applications have been tested with DNA purified using the PAXgene Blood DNA System?
FAQ ID - 3506
Can the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit be used with hybridization probes?
FAQ ID -2595
Can I use the QuantiTect Multiplex PCR Kits on the Roche LightCycler systems with TaqMan® probes or QuantiTect Assays?
FAQ ID -717
Can the QuantiFast Multiplex PCR Kits be used on Roche LightCycler systems with TaqMan® probes?
FAQ ID -1979
Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with QuantiFast Probe PCR Kits?
FAQ ID -1452
How is "Touchdown PCR" used to increase PCR specificity?
FAQ ID -75
What should the starting template DNA quality and quantity be for PCR?
FAQ ID -74
What kind of PCR products can be cloned with the QIAGEN PCR Cloning Kit?
FAQ ID -165
Why do I get smeared PCR products?
FAQ ID -87
Does QIAGEN sell Q-Solution separately?
FAQ ID -204
Do CoralLoad dyes supplied in various QIAGEN PCR Kits interfere with downstream applications?
FAQ ID -1745
Is Q-Solution required for PCR with QIAGEN's PCR kits?
FAQ ID -380
Have you tested the effect of inhibitors on PCR performance?
FAQ ID -818
What is the composition of the QIAGEN 10x PCR Buffer in Taq- and HotStarTaq DNA Polymerase Kits?
FAQ ID -606
Do you have to use a restriction enzyme to relieve ITR secondary structures and increase target accessibility?
FAQ ID - 3974
What is the recommended HpaII concentration?
FAQ ID - 3976
Can I use the lysate from the CGT Viral Vector Lysis kit and combine it with another PCR system?
FAQ ID - 3979

Do you have any recommendations on how to process my AAV samples?
FAQ ID - 3860
What can be done if lower and upper alignment markers used with the QIAxcel System or QIAxcel Advanced are not aligned correctly?
FAQ ID -1834
How do I safely inactivate biohazardous flow-through material?
FAQ ID -12
How should I store plant material for DNA isolation using the DNeasy Plant Kit?
FAQ ID -114
How can I precipitate genomic DNA using isopropanol?
FAQ ID -2953
How do I perform a DNA precipitation to concentrate my sample?
FAQ ID -305
What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?
FAQ ID -728
What is the composition of buffer AE?
FAQ ID -730
Do I need to have EDTA in the buffer in which I am going to store my isolated genomic DNA?
FAQ ID -754
Do you have a protocol for the isolation of DNA from tofu?
FAQ ID -932
Does the Epitect Bisulfite Kit also work on DNA extracted from plants?
FAQ ID -1209
What QIAGEN kit can I use to isolate DNA from food products to test for genetically modified organisms (GMOs)?
FAQ ID -371
After bisulfite conversion, can the DNA be stored?
FAQ ID -2409
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