Product FAQ - QIAGEN



Product FAQ Bookmark Share more.. You are not authorized to download the resource Sort options Sort alphabetically Sort by relevance Which real-time PCR kits are recommended downstream of the QuantiTect Whole Transcriptome Kit? FAQ ID -1592 View What primers are used in the reverse-transcription step of the QuantiTect Whole Transcriptome procedure? FAQ ID -1584 View Can the AllPrep DNA/RNA/Protein Mini Kit be used with fibrous or lipid tissues? FAQ ID -1579 View What is the maximum amount of RNA that can be used for amplification with the QuantiTect Whole Transcriptome Kit? FAQ ID -1591 View What starting amount of RNA purified using an miRNeasy Kit should be used with the miScript System? FAQ ID -1597 View Can RNA purified from formalin-fixed, paraffin-embedded (FFPE) tissue be used with the QuantiTect Whole Transcriptome Kit? FAQ ID -1583 View Can samples in Allprotect Tissue Reagent undergo repeated freeze-thaws without degradation of DNA, RNA, and protein? FAQ ID -1575 View Can the QuantiFast Probe PCR +ROX Vial Kit be used on the ABI PRISM 7900 and Applied Biosystems 7500 cyclers? FAQ ID -1555 View Does Buffer APP in the AllPrep DNA/RNA/Protein Mini Kit contain acetone? FAQ ID -1576 View Does cDNA amplified with the QuantiTect Whole Transcriptome Kit correspond to full-length RNA transcripts? FAQ ID -1585 View Can tissue incubated in Allprotect Tissue Reagent be used for morphological studies? FAQ ID -1571 View Can less than 10 ng RNA be used with the QuantiTect Whole Transcriptome Kit? FAQ ID -1590 View Can cDNA prepared by any method be used as starting material in the ligation reaction of the QuantiTect Whole Transcriptome Kit? FAQ ID -1589 View Can Allprotect Tissue Reagent be used to stabilize RNA, DNA and proteins in cells, bacteria, yeast, and plants? FAQ ID -1574 View Can degraded RNA be used with the QuantiTect Whole Transcriptome Kit? FAQ ID -1586 View How much cDNA is sufficient for real-time PCR detection of miRNA transcripts generated with the miScript System? FAQ ID -1598 View How many samples can be run in parallel with the Rotor-Gene Q real-time PCR instrument? FAQ ID -1519 View What main input voltage is required for the QIAxcel Analyzer? FAQ ID -1821 View Is there a quality difference between CompactPrep Plasmid Mega and Giga and the CompactPrep Plasmid Midi and Maxi Kits? FAQ ID -1882 View The Gel Cartridge is installed and all status indicators for the QIAxcel System are good but when clicking the "RUN" button, nothing happens. The green highlight goes down the Sequence? FAQ ID -1850 View Why does the copy function not apply relative to migration time and normalized area values after loading the DNA size marker table and adding the alignment marker peaks on the QIAxcel System? FAQ ID -1832 View Can customers use their own DNA size marker with the QIAxcel System and if yes, what concentration should be used? FAQ ID -1841 View Can data be saved at once with the QIAxcel System after analysis of all samples in a 96-well PCR plate? FAQ ID -1861 View How can a positive threshold be set when running the QIAxcel System? FAQ ID -1843 View How long will the intensity calibration procedure on the QIAxcel System take? FAQ ID -1826 View Will the QIAxcel System run using a green Data Review Software Key? FAQ ID -1853 View When should an intensity calibration be re-performed on the QIAxcel System? FAQ ID -1825 View Can the QIAxcel System or QIAxcel Advanced be connected to a network? FAQ ID -1862 View How can a clogged capillary channel be identified when running samples on the QIAxcel System or QIAxcel Advanced? FAQ ID -1836 View Can the QIAxcel software be loaded to another PC to view the data generated with the QIAxcel System? FAQ ID -1855 View Can multiple QIAxcel Systems be run from one computer? FAQ ID -1860 View How often should the solutions in the Buffer Tray for the QIAxcel System or QIAxcel Advanced be replaced? FAQ ID -1840 View How often should the alignment marker for the QIAxcel System be changed? FAQ ID -1839 View If the M500 method is used with the QIAxcel System, does a new DNA reference marker table have to be created? FAQ ID -1856 View In the results table generated with the QIAxcel System, what does "normalized area" mean? FAQ ID -1854 View What is the composition of buffer OCD? FAQ ID -423 View What is the composition of buffer QN? FAQ ID -414 View What is the composition of buffer OCL? FAQ ID -422 View What is the composition of buffer OL2? FAQ ID -424 View Can the QIAamp MinElute Virus Kits be used to extract viral nucleic acid from cell culture supernatant? FAQ ID -472 View Can RNAi experiments be performed in Drosophila? FAQ ID -436 View How is the RNeasy Lipid Tissue Mini Kit different from the RNeasy Mini Kit? FAQ ID -468 View Has RNAi been successful using siRNA in Zebrafish and Xenopus? FAQ ID -400 View Are Northern Blots sensitive enough to detect siRNA-induced gene silencing? FAQ ID -403 View Can I use the RNeasy MinElute Cleanup Kit to clean up my in vitro transcription reaction? FAQ ID -429 View What is the composition of buffer OEB? FAQ ID -420 View Why is the pQE DNA provided in QIAexpress Kits blue in color? FAQ ID -487 View What is the minimum elution volume when using the RNeasy Micro Kit? FAQ ID -428 View What is the maximum amount of starting material that can be processed with the RNeasy Micro Kit? FAQ ID -426 View What is the minimum elution volume when using the RNeasy MinElute Cleanup Kit? FAQ ID -432 View What is the smallest sample size that can be used with RNeasy Mini Kits? FAQ ID -485 View What is a homogeneous LiquiChip assay? FAQ ID -476 View What detection reagents (reporter fluorophores) do you recommend for the LiquiChip System? FAQ ID -480 View What are the excitation and emission wavelengths of the reporter and classification lasers of the LiquiChip Instrument? FAQ ID -479 View What is the binding capacity of the RNeasy MinElute Cleanup Kit column? FAQ ID -430 View What is the composition and size of the LiquiChip Beads? FAQ ID -475 View What is the binding capacity of the RNeasy Micro Kit column? FAQ ID -427 View Is dihydrofolate reductase (DHFR) immunogenic? FAQ ID -471 View What are some of the assays that are adaptable to the LiquiChip System? FAQ ID -477 View My QuantiTect Master Mix did not freeze at degrees. Is it still ok to use? -20 View I want to start with gene silencing (RNAi) experiments. Do you have informational literature? FAQ ID -580 View Is it possible to use less than 1 mg of protein with the PhosphoProtein Purification Kit? FAQ ID -599 View Is it possible to use a QIAzol lysate for the isolation of RNA from tissue on the BioRobot EZ1? FAQ ID -573 View What is a quenched FRET assay? FAQ ID -542 View What do you recommend for isolating RNA from more than 100 mg of plant tissue? FAQ ID -570 View What happens if I spin my lysate on the RNeasy Spin Columns at maximum speed? FAQ ID -514 View How should I dissolve the lyophilized Anti-His and Tag Antibodies? -100 View Is it possible to perform a UNG treatment when using QuantiTect kits? FAQ ID -564 View Is it possible to clean up a methylation reaction containing bisulfite with QIAquick Cleanup Kits? FAQ ID -519 View Is it possible to isolate both RNA and recombinant 6xHis-tagged protein from the same sample? FAQ ID -532 View Why are my realtime PCR amplification plots hook-shaped? FAQ ID -587 View Why are samples centrifuged at 4°C after the addition of chloroform when using RNeasy Lipid Tissue Kits? FAQ ID -533 View How was it determined that the PhosphoSerine Antibody will bind phosphoserines only? FAQ ID -579 View If pQE40 is used to express a fusion protein with DHFR, can the DHFR be cleaved afterwards? FAQ ID -513 View Which chloroform should I use for the RNeasy Lipid Tissue Kit? FAQ ID -516 View Will pancreas tissue swim on top of RNAprotect Tissue Reagent? FAQ ID -536 View Will the 2x QIAGEN Multiplex PCR Master Mix freeze at -20°C? FAQ ID - 525 View Why does my realtime PCR assay quality decrease over time? FAQ ID -589 View What is the longest fragment that was cloned with the pQE UA Cloning Vector? -30 View What is the difference between BSA-free and BSA-containing RGS-His Antibody? FAQ ID -569 View When using QIAzol Lysis Reagent for RNA extraction, I see a pinkish colored aqueous phase! Is it OK? FAQ ID -534 View Where can I find the public Smith-Waterman homology search tool that you refer to on your siRNA online design page? FAQ ID -562 View What reaction volume should I use with QuantiTect Kits? FAQ ID -551 View What makes QIAGEN's 10x Taq and HotStarTaq DNA Polymerase PCR buffer superior? FAQ ID -566 View How many template copies can I detect using QuantiTect Kits in real-time PCR? FAQ ID -543 View Can I use RNAiFect for co-transfection of plasmid DNA and siRNA? FAQ ID -515 View Does DEPC harm RNA? FAQ ID -529 View Does endogenously expressed DAPase in human cells degrade proteins that are expressed in cell culture? FAQ ID -527 View Can the MAPK1 control siRNA be used for rat cells? FAQ ID -547 View Are the columns of the MinElute Reaction Cleanup-, Gel Extraction-, and PCR Purification Kit identical? FAQ ID -581 View After using your U/A PCR Cloning Kit I can detect colonies, which are light blue, and not really white. Why is that? FAQ ID -512 View Can I make a master mix with QuantiProbes, primers, and all other reaction components and store it for later use? FAQ ID -584 View Can I homogenize samples in QIAzol using the QIAshredder? FAQ ID -501 View Can I shorten the activation time for the HotStarTaq DNA Polymerase? FAQ ID -565 View Does QIAGEN sell 6xHis-tagged proteins besides the 6xHis Protein Ladder? FAQ ID -594 View Do you offer QIAshredder spin columns for the RNeasy Midi/Maxi Kit format? If not, what is the alternative? FAQ ID -560 View Do you provide control proteins for use with the PhosphoProtein Purification Kit? FAQ ID -596 View How long should the amplicon be if I am using QuantiTect SYBR Green detection? FAQ ID -553 View How is an antibody epitope determined? FAQ ID -593 View Do I need to calibrate my real-time cycler if I want to use Yakima Yellow? FAQ ID -540 View Do we recommend pooling siRNA sequences? FAQ ID -517 View Does the His-tag interfere with the purification procedure of the QIAGEN PhosphoProtein Purification Kit? FAQ ID -598 View Does the use of MAPK1 as a control in siRNA transfection experiments have secondary effects on the cell culture? FAQ ID -548 View Do you have a protocol to isolate RNA from blood and bone marrow using the BioRobot EZ1? FAQ ID -567 View Do you have any recommendations for using the PosphoProtein Kit with starting material other than cells? FAQ ID -597 View Do you have a protocol for isolating RNA from yeast using RNeasy? FAQ ID -535 View What is the run time for the BioSprint 96 DNA Blood protocol on the BioSprint 96 instrument? FAQ ID -622 View Will REPLI-g work at high temperatures? FAQ ID -656 View The Multiflow Dispense Head on my BioRobot is not dispensing the correct amount of liquid. What can I do? FAQ ID -652 View What is the smallest amount of DNA I can put into a 50 ul REPLI-g reaction? FAQ ID -663 View What is the recommended incubation time to stabilize tissue RNA in RNAprotect Tissue Reagent? FAQ ID -647 View What do you mean by “Quantification of locus representation” in REPLI-g WGA product? FAQ ID -698 View What is REPLI-g whole genome amplification? FAQ ID -654 View What is the average amount of DNA and RNA present in 1 ml normal serum? FAQ ID -635 View What is the biggest protein that can be synthesized with the EasyXpress Protein Synthesis System? FAQ ID -601 View What is the length of the MDA whole genome amplification product? FAQ ID -690 View What is the expected yield of genomic DNA isolated from bacteria with the DNeasy Blood & Tissue or QIAamp DNA Mini Kit? FAQ ID -632 View Is it possible to perform laser microdissection with tissues stabilized with RNAprotect Tissue reagent? FAQ ID -608 View Is it possible to synthesize membrane proteins using the EasyXpress Protein Synthesis Kit? FAQ ID -602 View What are Elution Microtube adapters and Microplate adapters used in the DirectPrep 96 Miniprep Kit, and how can I order them? FAQ ID -646 View What are the advantages of REPLI-g over conventional DNA sample processing and amplification methods? FAQ ID -686 View Why do I get amplification in a negative control DNA tube using the REPLI-g Kit for WGA? FAQ ID -675 View Why did you choose MAPK1 as a positive control in the QIAGEN RNAi Human/Mouse Control Kit? FAQ ID -604 View Will the PhosphoSerine antibody recognize Serine residues if the neighboring residues are Leucine or Isoleucine? FAQ ID -644 View Will TA cloning work with REPLI-g WGA product? FAQ ID -672 View Will DNA from chromosomes collected in TE with a drop of mineral oil work with REPLI-g amplification? FAQ ID -664 View What is the stability of the REPLI-g MDA product? FAQ ID -693 View When delivering liquid to a 96-well plate using the MultiFlow Dispenser Head on my BioRobot , one or more rows in the plate contains less liquid per well than specified by the protocol. What is going wrong? FAQ ID -653 View Where should I add a dye-label modification on the siRNA? FAQ ID -648 View What types of materials from environmental samples could interfere with WGA? FAQ ID -666 View Can I stabilize RNA in bacteria grown on solid media or substrate using the RNAprotect Bacteria Reagent? FAQ ID -615 View Can I re-amplify whole genome amplification product? FAQ ID -670 View Can I use another lysis buffer than the one supplied in the PhophoProtein Purification Kit? FAQ ID -645 View Can I run less than 15 samples on the BioSprint 15 instrument? FAQ ID -621 View Can the QIAshredder Maxi Spin Columns from the DNeasy Plant Maxi Kit be used for RNeasy samples? FAQ ID -616 View Do you have an off-the shelf diagnostics kit for WGA? FAQ ID -678 View Are there components in biological samples that can inhibit the REPLI-g reaction? FAQ ID -667 View Are the BioSprint 15/96 instruments stand-alone workstations? FAQ ID -620 View Can the computer for the LiquiChip Workstation be networked? FAQ ID -629 View Are QIAGEN EZ Competent Cells of the QIAGEN PCR Cloning Kit dam+ or dam-? FAQ ID -638 View Are samples that are contaminated with high molecular weight DNA (e.g. from a paramedic or police officer) a problem with REPLI-g amplification? FAQ ID -683 View Can BAC DNA prepared with the R.E.A.L. Prep 96 Plasmid Kit be used for high-throughput sequencing? FAQ ID -643 View Can I do whole genome amplification from brain tissue using REPLI-g? FAQ ID -669 View Can I do whole genome amplification from mitochondrial DNA using REPLI-g? FAQ ID -668 View Can FTA paper for dried blood spots be used with REPLI-g? FAQ ID -685 View How can I quantify the amount of REPLI-g DNA I have amplified? FAQ ID -694 View Can I amplify from plasma or serum samples using the REPLI-g Mini/Midi kits? FAQ ID -671 View How should I store the REPLI-g amplification product? FAQ ID -692 View Is 2 kb the minimal gDNA fragment size for REPLI-g Whole Genome Amplification? FAQ ID -682 View How long can I store bacterial culture stabilized in RNAprotect Bacteria Reagent? FAQ ID -614 View Do I need to clean up the REPLI-g amplification product for downstream analysis? FAQ ID -691 View Do you have a kit for RNA isolation from any kind of sample type? FAQ ID -627 View Does REPLI-g technology for whole genome amplification work with paraffin embedded samples? FAQ ID -673 View How can I determine the quality of my REPLI-g amplified products? FAQ ID -695 View How can I decrease ribosomal RNA (rRNA) contamination using Oligotex? FAQ ID -642 View Do you know of anything in the buffers/DNA sample that can cause bias in REPLI-g WGA? FAQ ID -684 View Do you have a protocol for the purification of DNA from rodent tail using the BioSprint System? FAQ ID -979 View Do you have a protocol for the isolation of DNA from epithelial cells mixed with sperm cells? FAQ ID -926 View Do you have a protocol for isolation of genomic DNA from saliva using the DNeasy Blood & Tissue Kit? FAQ ID -931 View Do you have a protocol for purification of DNA from clotted blood using the FlexiGene DNA Kit? FAQ ID -973 View Do you have a protocol for Streptokinase treatment of clotted blood? FAQ ID -912 View Do you have a protocol for the isolation of total nucleic acids from animal and human cells on the BioRobot EZ1? FAQ ID -974 View Do you have a protocol for the isolation of total RNA from plant tissue using QIAGEN-tips? FAQ ID -949 View Do you have a protocol for the isolation of total RNA from buccal swabs? FAQ ID -956 View Do you have a protocol for the isolation of viral DNA from stool? FAQ ID -929 View Do you have a protocol for the isolation of total RNA from plants using the RNeasy 96 Kit? FAQ ID -951 View Do you have a protocol for the isolation of DNA from formalin-preserved stool samples? FAQ ID -922 View Do you have a protocol for the isolation of genomic DNA from fungi? FAQ ID -911 View Do you have a protocol for the isolation of genomic DNA and/or proteins from fatty tissue treated with QIAzol? FAQ ID -955 View Do you have a protocol for the purification of DNA from cells using the BioSprint System? FAQ ID -977 View Do you have a protocol for the purification of DNA from dried blood spots using the BioSprint System? FAQ ID -982 View Do you have a protocol for the preparation of tissues preserved in RNAprotect Tissue Reagent for pathological studies? FAQ ID -950 View Do you have a protocol for the purification of DNA from buccal swabs using the BioSprint System? FAQ ID -983 View Do you have a protocol for isolation of genomic and viral DNA from lymphocytes using QIAGEN Genomic-tips? FAQ ID -901 View Do you have a protocol for isolation of genomic and plasmid DNA from cell cultures using QIAGEN Genomic-tips? FAQ ID -902 View Do you have a protocol for transfection of adherent cells with low concentrations of siRNA using RNAiFect Transfection Reagent? FAQ ID -970 View Do you have a protocol for GMO testing of food samples? FAQ ID -921 View Do you have a protocol for isolation of bacterial DNA from soil? FAQ ID -923 View Do you have a protocol for isolation of genomic DNA from dried blood spots using the QIAamp 96 DNA Blood Kit? FAQ ID -919 View Do you have a protocol for isolation of genomic DNA from insects? FAQ ID -904 View Do you have a protocol for detection of HBV DNA by PCR? FAQ ID -915 View Does QIAGEN sell a vector with a T7 promotor for use with the EasyXpress Protein Synthesis Insect Kit? FAQ ID -937 View Can the BioSprint instruments be placed in a cool room? FAQ ID -938 View Do you have a protocol for Acetyl Cysteine (NALC) treatment of viscous samples? FAQ ID -913 View Do you have a protocol for co-transfection of adherent cells with siRNA and plasmid DNA? FAQ ID -969 View Do you have a protocol for adding new reporter dyes to the ABI PRISM 7900 Sequence Detection System? FAQ ID -965 View Do you have a protocol for cleanup of non-radioactive DNA samples? FAQ ID -945 View Do you have a protocol for radioactive labelling of cDNA? FAQ ID -960 View Do you have protocols for multiple extractions of DNA fragments from agarose gels? FAQ ID -944 View Why is it not recommended to stabilize cells with RNAprotect Tissue Reagent? FAQ ID -941 View Do you have a protocol for the detection of Bordetella pertussis DNA by PCR? FAQ ID -914 View Do you have a protocol for the isolation of DNA from cultured cells using the BioRobot EZ1? FAQ ID -990 View Do you have a protocol for performing quantitative multiplex PCR on the LightCycler 2.0 system? FAQ ID -966 View Do you have a protocol for long-term, siRNA-mediated gene silencing using HiPerFect Transfection Reagent? FAQ ID -972 View Do you have a protocol for manual purification of 6xHis-tagged proteins expressed in E. coli using Ni-NTA Superflow? FAQ ID -967 View Do you have a protocol for purification of DNA from cultured animal cells using the DNeasy 96 Blood & Tissue Kit? FAQ ID -934 View Do you have a protocol for purifying 2x96 PCR samples simultaneously with the QIAquick 96 PCR Purification Kit? FAQ ID -935 View Do you have a protocol for purification of DNA fragments from Cy3/Cy5 dye-label reactions? FAQ ID -947 View Do you have a protocol for polyacrylamide gel analysis of oligonucleotides? FAQ ID -961 View Do you have a protocol for isolation of genomic DNA from plants and filamentous fungi using QIAGEN Genomic-tips? FAQ ID -905 View Do you have a protocol for isolation of genomic DNA from flies using QIAGEN Genomic-tips? FAQ ID -903 View What are exo-resistant random hexamers used in the REPLI-g reaction? FAQ ID -710 View The Sigma Centrifuge makes a loud noise at low speeds which disappears at higher speeds, when using the 2x96-well plate rotor. What is the problem? FAQ ID -776 View Is RNAprotect Bacteria Reagent compatible with the RNeasy Mini Kit? FAQ ID -797 View What is the basic technology behind the Strep-tag Protein Purification System? FAQ ID -740 View What are the expected average DNA yields from plant extractions on the BioSprint 96? FAQ ID -771 View If template abundance differs significantly between housekeeping and target gene, are both amplified equally efficient with the QuantiTect Multiplex Kits? FAQ ID -718 View How to quantitate DNA purified using the BioSprint 96? FAQ ID -773 View How to store DNA from forensic samples eluted into water on the EZ1 biorobot in TE buffer instead? FAQ ID -734 View Is it possible to use LiquiChip beads for FACS applications? FAQ ID -733 View Is it possible to buy the EZ Competent Cells of the QIAGEN PCR Cloning Plus Kit separately? FAQ ID -763 View Is GFP excited and detected by the green laser of the LiquiChip System? FAQ ID -737 View Which Qproteome or Protein Fractionation Kits from QIAGEN are compatible with tissue samples? FAQ ID -755 View Why are some of my RNAprotect-stabilized samples frozen at 0ºC while others are not? FAQ ID -758 View Why are the QuantiTect and QuantiFast Multiplex PCR Kits limited to triplex real-time PCR on some cyclers? FAQ ID -715 View Why should DNA or cDNA targets be less than 250 bp long for real-time PCR? FAQ ID-751 View Where can I find info on compatible reporter dyes for use in real-time multiplex PCR using the QuantiTect Multiplex PCR Kits? FAQ ID -719 View Why might my gene of interest drop out during WGA if it is not near a telomere or centromere? FAQ ID -703 View Will the random hexamers in the REPLI-g reaction interfere with downstream analysis? FAQ ID -713 View Why is there no detection of free reporter fluorescence in the LiquiChip System? FAQ ID -732 View What is the cell number range one can use with the FastLane Cell cDNA Kit for real-time RT-PCR? FAQ ID -782 View What negative (unphosphorylated) and positive (phosphorylated) control proteins do you recommend for use with the PhosphoProtein Purification Kit? FAQ ID -779 View What is the enzyme used in the REPLI-g reaction? FAQ ID -704 View What type of Sequencing Services does QIAGEN offer? FAQ ID -781 View Where can I find a copy of the report files generated by running accessory protocols "Dilutor Delivery (monthly)" and "High-Speed Pipetting Sytem (monthly)" on my BioRobot 9604? FAQ ID -766 View What is the general sensitivity level of the LiquiChip System? FAQ ID -731 View Can QIAGEN's Taq- and HotstarTaq DNA Polymerases be used for cycle sequencing? FAQ ID -741 View Can I use the PhosphoProtein Purification Kit to purify phosphorylated peptides of around 1 kD size? FAQ ID -778 View Can RNAprotect Tissue Reagent be used to stabilize tissue which has already been flash-frozen? FAQ ID -757 View Can lyophilized plant material be used for DNA extraction on the BioSprint 96? FAQ ID -772 View Can the REPLI-g WGA enzyme work with labeled dNTPs? FAQ ID -706 View Does the QIAamp DSP Virus Kit require the QIAvac 24 Plus, or can I use it with my own laboratory vacuum system? FAQ ID -789 View Can the MAPK1 siRNA of the RNAi Human/Mouse Starter Kit be purchased separately? FAQ ID -744 View Can the QIAvac 96 vacuum manifold be used with DirectPrep 96 plates? FAQ ID -722 View Are the buffers in the Ni-NTA Fast Start Kit the same as the ones for use with Ni-NTA purchased separately? FAQ ID -791 View Any data on the fidelity of the REPLI-g MDA technique? FAQ ID -707 View Are Centromeres and Telomeres amplified using REPLI-g WGA? FAQ ID -702 View Can I use my own primers for REPLI-g WGA to amplify a specific chromosomal region? FAQ ID -712 View Can I do 4-plex real-time PCR, on the ABI PRISM 7000, 7700, or 7900 using the QuantiTect Multiplex PCR Kit? FAQ ID -716 View How can one perform multiplex real-time PCR analysis using the LightCycler 2.0? FAQ ID -780 View How can I be sure that I am harvesting my induced bacterial culture at the best time point for protein expression? FAQ ID -788 View How do the FastLane Cell cDNA Kit and QuantiTect Reverse Transcription Kit eliminate genomic DNA contamination? FAQ ID -783 View How many cells can be used with the FastLane Cell cDNA Kit? FAQ ID -796 View Do you have an antibody for Western Blot Analysis of MAPK1 gene knockdown using your MAPK1 control siRNA? FAQ ID -746 View Can frozen blood be used for whole genome amplification with REPLI-g? FAQ ID -794 View Do I have to switch off the laser of the LiquiChip Workstation overnight? FAQ ID -736 View How many reporter dyes can be used in the same analysis using the LiquiChip Workstation? FAQ ID -738 View Do I need to determine limiting primer concentrations with the QuantiTect Multiplex PCR Kits? FAQ ID -714 View Do you synthesize thiophosphate modified random hexamers? FAQ ID -711 View Has anyone verified whole genome amplification accuracy with Sequencing? FAQ ID -701 View Do you have a protocol for the fixation of cells transfected with fluorescently labeled siRNA? FAQ ID -793 View How best to handle the light sensitive LiquiChip reagents? FAQ ID -735 View Do you have information on the use of recombinant DNA and RNA as absolute standards for realtime RT-PCR? FAQ ID -729 View Is it possible to transfect DNA into insect cell lines such as Drosophila melanogaster S2? FAQ ID -397 View Is it possible to isolate free genomic DNA using the QIAamp UltraSens Virus Kit? FAQ ID -358 View Is it possible to store TAGzyme enzymes at°C? -80 View I accidentally added RNAprotect Tissue Reagent to my cells, and now the cells are difficult to pellet. What can I do? FAQ ID -364 View What are the calculated molecular weights of the TAGZyme enzymes? FAQ ID -329 View How complete is the removal of DAPase in the TAGZyme process? FAQ ID -319 View How long is fluorescence detectable in cells after transfection with fluorescently labeled siRNAs? FAQ ID -392 View How do I submit a siRNA order by telephone or online? FAQ ID -399 View How many transfections can I perform with 20 nmol of HPP grade siRNA? FAQ ID -367 View Which primers can I use for sequencing pQE-expression vector constructs? FAQ ID -343 View What is the standard assay volume recommended for LiquiChip assays? FAQ ID -330 View What is the acceptable maximum g force for centrifugation of Xa Removal Resin? FAQ ID -320 View What is the lowest elution volume that can be used with QIAprecipitator Midi and Maxi Modules? FAQ ID -307 View What is the composition of Buffer FG3? FAQ ID -356 View Can I use ethanol instead of isopropanol for DNA precipitation when using HiSpeed Plasmid Kits? FAQ ID -354 View 303 - Can I use my own lysis buffer with the DNeasy Blood & Tissue or QIAamp DNA Mini Kit? View Can I use a partial MinElute 96 UF plate? FAQ ID -355 View Can I use the RNeasy Mini Kit or RNeasy 96 Kit with fewer than 100 cells? FAQ ID -372 View Can I use water for elution from the QIAprecipitator of the HiSpeed Plasmid Kits? FAQ ID -306 View Are the QIAprecipitator Midi and Maxi Modules of the HiSpeed Plasmid Kits interchangeable? FAQ ID -308 View Apart from the antibody itself, does the Penta-His Biotin Conjugate solution contain any protein? FAQ ID -359 View Are QIAGEN monoclonal Anti His Antibodies glycosylated? FAQ ID -334 View Can I perform in-vitro transcription with the pDrive Cloning Vector from the QIAGEN PCR Cloning Kit? FAQ ID -332 View Do the QuantiTect SYBR Green Kits contain a stabilization reagent that inhibits the sensitivity of real-time PCR? FAQ ID -328 View Do I need to anneal, deprotect or desalt my QIAGEN siRNA? FAQ ID -398 View How are siRNAs introduced into C. elegans? FAQ ID -396 View Do pQE vectors contain the CAT gene? FAQ ID -362 View How can I improve the expression of proteins containing hydrophobic regions? FAQ ID -339 View How can I check if any residual proteins remain on the Ni-NTA Agarose matrix after elution? FAQ ID -324 View Do freeze-thaw cycles affect the stability of the U-overhang on the pDrive Cloning Vector? FAQ ID -323 View Can RNAprotect Tissue Reagent be used to store previously isolated RNA? FAQ ID -337 View Can MinElute 96 UF PCR be used to purify PCR samples which contain mineral oil? FAQ ID -360 View Does SYBR Green dye present in QuantiTect kits interfere with ethidium bromide staining? FAQ ID -312 View Do any of the buffers in the HotStarTaq DNA Polymerase Kit contain Triton? FAQ ID -327 View Do any of the kit components or the product packaging of the QIAamp 96 DNA Blood Kit contain latex? FAQ ID -318 View What data documentation does QIAGEN's Sequencing Service provide for customers? FAQ ID -832 View What are the sequences of the FlexiTube siRNAs? FAQ ID -851 View My BioRobot 9604 came with several Accessory Applications. Is it recommended I run these protocols? FAQ ID -872 View What is the proper way to turn on or turn off the EZ1 instrument? FAQ ID -870 View What is the Energy Mix in the EasyXpress Kits? FAQ ID -846 View What is the concentration of the primers in a reaction using the QuantiTect Primer Assays? FAQ ID -850 View I would like to sequence my siRNA constructs. Does QIAGEN offer Sequencing Services for cloned siRNA? FAQ ID -829 View Is it possible to use frozen cell pellets with the FastLane Cell cDNA Kit? FAQ ID -835 View Is it possible to elute plasmid DNA from the QIAprep Spin Miniprep columns with buffer containing Potassium Phosphate? FAQ ID -854 View Is it possible to perform immunoprecipitation (IP) directly on protein fractions from the Qproteome Cell Compartment Kit? FAQ ID -855 View Is it possible to adjust the Lysis Buffer volume used with the Qproteome Bacterial Protein Preparation Kit? FAQ ID -841 View Is it possible to use QIAGEN's pQE-TriSystem Vectors with the EasyXpress Protein Synthesis Insect Kit? FAQ ID -876 View Is there a stopping point in the protocol for Suspended Cells using the FastLane Cell cDNA Kit? FAQ ID -837 View Is the random biotin labeling of proteins expressed with your EasyXpress Biotin Labeling Kit as sensitive as labeling with radioactivity? FAQ ID -875 View Will biotin detection assays be efficient when doing in vitro translation from E. coli lysates with the EasyXpress Site-Specific Biotin Kit? FAQ ID -878 View Why would clumps occur following the addition of Buffer P2 when using LyseBlue Reagent in a plasmid preparation? FAQ ID -862 View Will acetone precipitation recommended in the Qproteome Protocols denature Protein? FAQ ID -807 View Will site-specific biotin labeling with the EasyXpress Site-Specific Biotin kit alter the structure and function of my protein? FAQ ID -877 View Will the sequences of the QuantiTect Primer Assays be provided? FAQ ID -804 View Will the FastLane Cell cDNA Kit work with suspension cells? FAQ ID -801 View Which resin is used in the QIAexpress Ni-NTA Fast Start Columns? FAQ ID -836 View What publicly funded Genome Sequencing Projects has QIAGEN's Sequencing Service been involved in? FAQ ID -815 View What is the typical yield of site-specific labeled protein generated using the EasyXpress Site-Specific Biotin Kit? FAQ ID -873 View What programs does your Sequencing Service use to ensure accurate base calling, Sequence Quality, and Alignment? FAQ ID -831 View Where can I find a copy of the report file generated by running 'Maintenance Protocol I' on my BioRobot MDx? FAQ ID -806 View Which fraction will contain soluble mitochondrial proteins using the Qproteome Cell Compartment Kit? FAQ ID -839 View Which fraction will contain endosomal, microsomal and lysosomal proteins using the Qproteome Cell Compartment Kit? FAQ ID -808 View Where can I find QIAGEN products for a specific gene or gene product? FAQ ID -803 View Can the QuantiTect Primer Assays be used for detection of genomic DNA? FAQ ID -811 View Do I need to use RNase inhibitors with the RNeasy Kits? FAQ ID -813 View Do the Buffers CE1 - CE4 of the Qproteome Cell Compartment Kit contain DTT or any reducing agent? FAQ ID -824 View Can the FastLane Cell cDNA Kit also be used for qualitative end-point PCR? FAQ ID -834 View Can the FastLane Cell cDNA Kit be used with tissue? FAQ ID -821 View Do you have a protocol for the isolation of bacteriophage P1 derived constructs with any of your plasmid kits? FAQ ID -883 View Do you have a protocol for the isolation of BAC DNA using the QIAGEN Plasmid Midi Kit? FAQ ID -881 View 895 - Do you have a protocol for the isolation of endotoxin-free plasmid DNA using the QIAGEN Plasmid Midi Kit? View Do you have a protocol for isolation of plasmid DNA using the Sigma Centrifuge 4? -15 View Can Buffer R1 of the R.E.A.L. Prep 96 Plasmid Kit be replaced with Buffer P1? FAQ ID -823 View Are there specific recommendations for performing RT-PCR on RNA isolated from paraffin-embedded samples? FAQ ID -828 View Can I stabilize adipose tissue using RNAprotect Tissue Reagent prior to RNA isolation? FAQ ID -856 View Can I use the PhosphoProtein Purification Kit for the isolation of phosphorylated proteins from yeast? FAQ ID -838 View Can I use QIAGEN's pQE vectors as templates in the EasyXpress Biotin Labeling Kits? FAQ ID -874 View Can I use my gene-specific primers with the FastLane Cell cDNA Kit and the QuantiTect Reverse Transcription Kit? FAQ ID -812 View Can I use LyseBlue with R.E.A.L. Prep 96, QIAwell Ultra, or QIAprep 96 Turbo Miniprep Kits for the BioRobot systems? FAQ ID -863 View Do you have information on in vivo RNA interference experiments? FAQ ID -817 View Do you sell the ATP-Dependent Exonuclease of the QIAGEN Large-Construct Kit separately? FAQ ID -825 View I cannot see the LCD display fonts on the Sigma Centrifuge. How do I adjust the contrast? FAQ ID -822 View How long does a typical in vitro translation reaction take with your large-scale EasyXpress protein synthesis kits? FAQ ID -880 View How much protein can be produced with the EasyXpress Protein Synthesis Mega Kit? FAQ ID -843 View Do you have a protocol for the isolation of very low-copy plasmids from Streptomyces spp.? FAQ ID -893 View Do you have a protocol for the isolation of plasmid DNA from Citrobacter freundii? FAQ ID -885 View Do you have a protocol for the isolation of plasmid DNA from Corynebacterium glutamicum? FAQ ID -886 View Do you have a protocol for the isolation of plasmid DNA from Borrelia spp.? FAQ ID -884 View Do you have a protocol for the isolation of plasmid DNA from Lactobacillus spp.? FAQ ID -887 View Do you have a protocol for the isolation of plasmid DNA from yeast? FAQ ID -853 View Do you have a protocol for the isolation of single-stranded DNA from M13 phage using QIAGEN Plasmid Kits? FAQ ID -894 View Do you have a protocol for the isolation of plasmid DNA from Staphylococcus spp.? FAQ ID -890 View Do you have a protocol for the isolation of plasmid DNA from Oligotropha carboxidovorans? FAQ ID -888 View Do you have a protocol for the isolation of plasmid DNA from Proteus spp.? FAQ ID -889 View What are your recommendations for cotransfecting several plasmids? FAQ ID -124 View What are the recommended culture and buffer volumes for a very low-copy plasmid? FAQ ID -168 View Is mRNA isolation necessary for sensitive RT-PCR? FAQ ID -111 View When should carrier be used with the QIAamp DNA Mini or the DNeasy Blood & Tissue Kit? FAQ ID -100 View Which Anti-His Antibody is the most sensitive for my protein of interest? FAQ ID -172 View Why do I have to add beta-mercaptoethanol (beta-ME) to lysis Buffer RLT of the RNeasy Kits? FAQ ID -101 View What is the recommended culture medium for the QIAprep System? FAQ ID -154 View How can I eliminate contaminating protein in my Ni-NTA 6xHis-tag protein purification? FAQ ID -102 View How can I purify very small amounts of 6xHis-tagged protein using Ni-NTA technology? FAQ ID -134 View How can I improve ligation efficiency of DNA from a QIAEX II Gel Extraction Kit? FAQ ID -141 View How can I isolate RNA from 1 gram of plant tissue? FAQ ID -128 View How can I improve the performance of the HiSpeed QIAprecipitator module? FAQ ID -144 View What can I use to isolate RNA smaller than 200 nucleotides? FAQ ID -115 View What epitopes do the Anti-His Conjugates and Anti-His Antibodies recognize? FAQ ID -170 View What are the maximum culture volumes to use with the QIAGEN Plasmid Midi or Maxi Kit? FAQ ID -167 View How should RNeasy Kits be stored and how long are they stable? FAQ ID -103 View Is it possible to cleave the 6xHis-tag from an expressed protein? FAQ ID -140 View How should QIAGEN Plasmid Purification Kits be stored and for how long? FAQ ID -192 View What are the features and benefits of the QIAexpress 6xHis Tag System? FAQ ID -193 View Do you have transfection data for QIAGEN Transfection Reagents? FAQ ID -158 View Do you have data showing effects of sample size on DNA yield and purity using the DNeasy 96 Blood & Tissue kit? FAQ ID -129 View Does QIAGEN offer vectors for direct cloning of PCR products? FAQ ID -146 View How can I avoid variable transfection efficiencies with SuperFect Reagent? FAQ ID -179 View Can DyeEx be used to clean up labeled DNA other than sequencing templates? FAQ ID -137 View Are QIAamp DNA isolation kits suitable for apoptosis studies? FAQ ID -149 View Can I use a QIAfilter Cartridge for purifying large plasmids? FAQ ID -121 View Can I use Omniscript or Sensiscript RT's at a higher temperature? FAQ ID -116 View How do you ensure that RNeasy buffers are RNase-free? FAQ ID -113 View How can I purify DNA from soil, food and sewage samples for PCR? FAQ ID -118 View How should I store Anti·His and Tag·100 Antibodies? FAQ ID -194 View How do I prepare an insert for pQE vectors? FAQ ID -185 View How can I obtain DNA-free RNA using an RNeasy Midi or Maxi Kit? FAQ ID -143 View What are the compatibilities of different reagents with Ni-NTA matrices? FAQ ID -49 View How can I prevent running out of M48 Kit reagents before the full number of samples are processed? FAQ ID -1042 View Do you have a protocol for the isolation of genomic DNA from paraffin-embedded samples on the BioRobot M48? FAQ ID -1019 View Is the TurboCapture Microplate Holder Type B compatible with the Biomek FX robotic workstation? FAQ ID -1040 View Is there a limit to the size of proteins that can be purified using the Qproteome Glycoprotein Kits? FAQ ID -1071 View Is it possible to scale up QuantiTect Reverse Transcription reactions to allow use of larger amounts of RNA? FAQ ID -1063 View Is it possible to use the PhosphoProtein Purification Kit in batch format? FAQ ID -1069 View What are the recommended storage conditions for the QuantiTect Reverse Transcription Kit and its components? FAQ ID -1077 View What is the difference between TurboCapture Microplate Holders Type A and Type B? FAQ ID -1038 View What blocking reagents do you recommend for chemiluminescent detection using Penta-, Tetra-, and RGS-His antibodies? FAQ ID -1093 View Is Factor SB of the HotStar HiFidelity Polymerase Kit the same as Factor MP of the QIAGEN Multiplex PCR Kit? FAQ ID -1049 View How can I transfect siRNA into insect cells such as Drosophila melanogaster S2? FAQ ID -1046 View How can I tell TurboCapture Microplate Holder Type A from Type B? FAQ ID -1039 View How much template can I use in the reaction and what is the maximum volume of template that can be used in the QuantiTect Kits? FAQ ID -1086 View How much dye-labeled siRNA should I use to monitor transfection efficiency when using HiPerfect Transfection Reagent? FAQ ID -1058 View What is the error rate of HotStar HiFidelity DNA Polymerase? FAQ ID -1048 View Which QIAGEN kit do you recommend for purifying plasmid DNA suitable for transfection of sensitive cells? FAQ ID -1092 View Which kit should I use for RNA isolation from Cartilage? FAQ ID -1026 View Where can I find the serial number on QIAGEN's BioRobots? FAQ ID -1064 View Which kit can be used for simultaneous purification of DNA and RNA from biopsies? FAQ ID -1089 View Why is there RNA in the DNA eluate when using the AllPrep DNA Mini Spin Column? FAQ ID -1044 View Why does my PCR product show up later when comparing the QuantiTect SYBR Green PCR Kits with Roche kits using the same annealing temperature? FAQ ID -1083 View Will the "classic" RNeasy Mini Kit be discontinued after the launch of the Allprep DNA/RNA Mini Kit? FAQ ID -1054 View Why does TA/UA cloning work with your proofreading HotStar HiFidelity DNA Polymerase? FAQ ID -1053 View Why do melting temperatures differ between PCR fragments amplified with QIAGEN's QuantiTect SYBR Green PCR Kits and Roche Kits? FAQ ID -1084 View What is the range of melting temperatures for the QuantiTect Primer Assay amplicons in the dissociation curve? FAQ ID -1079 View What is the protein loading capacity per column of the Qproteome Albumin/IgG Depletion Kit? FAQ ID -1090 View What is your recommendation for cleaning the TissueLyser Bead Dispenser (96-well format)? FAQ ID -1055 View What vacuum pressure should be applied using the QIAvac 24 Plus for the QIAamp DSP Virus Kit and Blood Mini Kit Protocols? FAQ ID -1033 View What is the resin in the Qproteome Murine Albumin and Qproteome Albumin/IgG Depletion Kits? FAQ ID -1034 View How can I prevent clogging of QIAfilter cartridges? FAQ ID -1060 View What is the volume of the eluate when using either spin or vacuum procedures with the QIAprep 96 Turbo Miniprep Kit? FAQ ID -1067 View Do I have to use a ramp time of 2°C/sec on the LightCycler when using Quantitect Primer Assays? FAQ ID -1065 View Does an activation time of 15 minutes influence the performance of the HotStarTaq Plus DNA Polymerase? FAQ ID -1050 View Do you have a protocol for the isolation of RNA from bacterial cultures using RNAprotect Bacteria Reagent and QIAzol Lysis Reagent? FAQ ID -1020 View Do you have a protocol for the isolation of DNA from mouse tails using MagAttract technology on BioRobot M96? FAQ ID -1007 View Do you have a protocol for the isolation of DNA from forensic samples using the BioRobot M48? FAQ ID -1017 View Can the USB Port of the BioSprint 96 instrument be used to transfer programs from a PC to the instrument? FAQ ID -1032 View Are your FlexiTube siRNAs preferentially designed to any particular region of the target gene? FAQ ID -1091 View Can PCR fragments generated with the EasyXpress Linear Template Kit be cloned into the pIX4.0 vector of the EasyXpress Protein Synthesis Insect Kit? FAQ ID -1078 View Can the QProteome O-Glycan Glycoprotein Kits be used for blood cells? FAQ ID -1036 View Can the Reverse Transcriptases of the QuantiTect Reverse Transcription Kit and the QuantiTect Probe RT-PCR Kit be used interchangeably? FAQ ID -1066 View Can the AllPrep RNA/Protein Kit be used with tissue? FAQ ID -1095 View Can the Blocking Reagent supplied with the Penta-His HRP Conjugate be used with other antibodies? FAQ ID -1073 View How can air bubbles in PhosphoProtein Purification Columns be avoided? FAQ ID -1070 View How can I increase low-copy plasmid DNA yields using QIAfilter Plasmid Kits? FAQ ID -1062 View How can I ensure complete genomic DNA removal when using the RNase-Free DNase Set? FAQ ID -1087 View Do you have information on the reproducibility of DNA yields from blood using the M48 BioRobot? FAQ ID - 1004 View Do you have a protocol for transient transfection of HEK 293 cells in 75 cm3 flasks? FAQ ID -1014 View Do you have a protocol for transient transfection of cells in 96-well plates using PolyFect Transfection Reagent? FAQ ID -1013 View Do you have a protocol for transient transfection of HeLa-S3 cells in 60 mm dishes using PolyFect? FAQ ID -1012 View Do you have a protocol for the isolation of total nucleic acids from cells on the M48 BioRobot? FAQ ID -1009 View Do you have a protocol for vacuum processing of blood samples with the QIAamp DNA Blood Midi or Maxi Kits? FAQ ID -1011 View Do you have a protocol for the isolation of total nucleic acids from tissue on the M48 BioRobot? FAQ ID -1008 View Do you have information on realtime RT-PCR performance of RNA isolated from monocytes on the M48 BioRobot? FAQ ID -1010 View Do you have information on DNA yields from blood with variable numbers of white blood cells using the M48 BioRobot? FAQ ID -1006 View Do you have information on PCR template preparation from preserved blood samples on the M48 BioRobot? FAQ ID - 1003 View Can the PAXgene Blood RNA Tubes undergo freeze/thaw cycles? FAQ ID -2471 View If I want to use BioMag particles, do I have to buy a QIAGEN magnet too or can I use one that I already have? FAQ ID -266 View Should I use Omniscript or Sensiscript for reverse transcription of low-copy mRNA? FAQ ID -297 View What are the main differences between other magnetic beads and the BioMag particles? FAQ ID -272 View My BioMag is about to expire, is it still any good? FAQ ID -268 View How magnetically responsive is BioMag? FAQ ID -265 View If I am using BioMag Streptavidin to capture biotinylated oligos, how can I then remove the oligos? FAQ ID -279 View Why is an ice-incubation step included during reaction set-up when following the QuantiTect RT-PCR but not the QuantiTect PCR protocol. FAQ ID -283 View What sequencing primers can I use with the pDrive cloning vector of the QIAGEN PCR Cloning Kit? FAQ ID -292 View What is the difference between the QIAamp MinElute Virus Spin and the MinElute Virus Vacuum Kit? FAQ ID -295 View What is the difference between the Fc specific versions of your secondary antibody BioMag particles and the non-specific? FAQ ID -275 View Can I use my own cycling conditions with the QIAGEN Multiplex PCR Kit? FAQ ID -287 View Can I select FITC labeled cells with BioMag? FAQ ID -278 View Can I use BioMag particles with any specific primary antibody? FAQ ID -273 View Are BioMag particles stable in other solvents? FAQ ID -267 View Can BioMag be used for positive and negative selection? FAQ ID -269 View How can I avoid poor immunolocalization morphology with Anti-His Antibodies? FAQ ID -200 View How can I prevent non-specific binding when using BioMag? FAQ ID -277 View How do I prevent bubbles from forming in my Ni-NTA agarose column? FAQ ID -285 View How can I purify mRNA from total RNA using an Oligotex Direct mRNA Kit? FAQ ID -201 View Is your Nuclease-Free Water fluorescence-free? FAQ ID -1291 View What quality level does your Nuclease-Free Water have? FAQ ID -1292 View How should the GelPilot DNA Loading Dye be handled? FAQ ID -1287 View Does GelPilot DNA Loading Dye contain ethidium bromide? FAQ ID -1286 View Can phenol/chloroform be placed in the MaXtract tube for extraction at a later time? FAQ ID -1299 View Are MaXtract tubes siliconized? FAQ ID -1298 View Should I input the dilution factor of my samples into the LiquiChip Analyser Software dilution factors of samples? FAQ ID -1211 View What cell lines have been tested with the Qproteome Plasma Membrane Protein Kit? FAQ ID -1205 View Is a saliva collection device provided in the RNeasy Protect Saliva Mini Kit? FAQ ID -1213 View Is it possible to increase nucleic acid concentrations when following protocols on the BioRobot EZ1? FAQ ID -1232 View Is RNAprotect Cell Reagent provided in the RNeasy Protect Cell Kit available separately? FAQ ID -1217 View Is it possible to use QIAGEN's pQE-Trisystem Vector with the EasyXpress Insect Kit II? FAQ ID -1225 View What vector do you recommend as template for in-vitro protein expression with the EasyXpress Insect Kit II? FAQ ID -1224 View What type of promoter should my template have for in-vitro protein expression with the EasyXpress Insect Kit II? FAQ ID -1223 View What is the maximum number of cells that can be used with the AllPrep RNA/Protein Kit ? FAQ ID -1208 View Will the Qproteome Plasma Membrane Protein Kit work with starting materials other than adherent mammalian cells? FAQ ID -1202 View What is the difference between various FlexiTube siRNAs listed for the same target gene, and which one should I choose? FAQ ID -1206 View What is the protein yield when using the Qproteome Plasma Membrane Protein Kit? FAQ ID -1201 View What is the size of the BioRobot EZ1? FAQ ID -1245 View Does anything have to be added to express post-translational modifications (PTMs) with the EasyXpress Insect Kit II? FAQ ID -1226 View Do you have a protocol for isolating cytoplasmic RNA from animal cells using the RNeasy Mini Kit? FAQ ID -1207 View Do you have a protocol for purification of miRNA from animal cells using the RNeasy Plus Mini Kit and RNeasy MinElute Cleanup Kit? FAQ ID -1258 View Can cells stabilized with RNAprotect Cell Reagent be used for cell sorting by flow cytometry (FACS)? FAQ ID -1216 View Can glycosylated proteins be expressed using the EasyXpress Insect Kit II? FAQ ID -1227 View Can RNAprotect Saliva Reagent be purchased separately? FAQ ID -1214 View Can you recommend a Reagent or Kit for stabilizing RNA in cultured cells? FAQ ID -1215 View Can the Strep-Tactin Magnetic Beads of the Qproteome Plasma Membrane Protein Kit be reused? FAQ ID -1204 View Can the Qproteome Plasma Membrane Protein Kit be used with tissue samples? FAQ ID -1200 View Can the RLT lysate of the BioSprint DNA Plant protocol be stored frozen? FAQ ID -1248 View How can I decontaminate the BioRobot EZ1? FAQ ID -1231 View How do you ensure that concentrated RNA is purified from saliva using the RNeasy Protect Saliva Mini Kit? FAQ ID -1212 View How much active protein can be expressed in-vitro using the EasyXpress Insect Kit II, and how much time does it take? FAQ ID -1222 View How many freeze-thaw cycles can the various reaction mixes in the EasyXpress Insect Kit II tolerate? FAQ ID -1219 View Do you have a protocol for the isolation of DNA from buccal cells using the BioRobot EZ1? FAQ ID -1260 View Do you have a protocol for transfection of differentiated macrophage cell lines (THP) with siRNA? -1 View How are plasma membrane containing vesicles eluted off the ligand in the Qproteome Plasma Membrane Protein procedure? FAQ ID -1203 View Which fixation method is used in the PAXgene Tissue System? FAQ ID - 3600 View What is the fixation reagent of PAXgene Tissue FIX? FAQ ID - 3601 View What is the stabilization reagent of PAXgene Tissue STABILIZER? FAQ ID - 3602 View What is the maximum tissue size that can be fixed in a PAXgene Tissue FIX Container (50 ml)? FAQ ID - 3604 View Why are two reagents used in the PAXgene Tissue System? FAQ ID - 3603 View Which conditions are recommended for the storage of tissues in PAXgene Tissue STABILIZER? FAQ ID - 3605 View Is it possible to process formalin-fixed and PAXgene Tissue fixed samples together in one run? FAQ ID - 3606 View Is it necessary to clean a processor normally used for formalin-fixed tissue before using it with PAXgene Tissue fixed tissue? FAQ ID - 3607 View Is it possible to integrate the PAXgene Tissue STABILIZER into tissue processing? FAQ ID - 3608 View Are immunohistochemistry (IHC) assays developed for formalin-fixed, paraffin-embedded (FFPE) tissues compatible with PAXgene Tissue fixed, paraffin-embedded (PFPE) tissues? FAQ ID - 3609 View How well is RNA integrity preserved in PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue? FAQ ID - 3611 View Is it possible to use formalin-fixed, paraffin-embedded (FFPE) kits and protocols to isolate biomolecules from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue? FAQ ID - 3610 View How well is DNA integrity preserved in PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue? FAQ ID - 3612 View Are special kits and protocols required to isolate biomolecules from PAXgene Tissue fixed, cryo-embedded (PFCE) tissue? FAQ ID - 3614 View What are the yield and integrity of nucleic acids isolated from blocks of PAXgene Tissue fixed, cryo-embedded (PFCE) tissue? FAQ ID - 3613 View Can proteins be extracted from PAXgene Tissue fixed specimens? FAQ ID - 3616 View Does the PAXgene Tissue RNA Kit co-purify small RNAs? FAQ ID - 3615 View Which kits and protocols can be used to isolate nucleic acids from microdissected PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) specimens? FAQ ID - 3617 View What is the PCR performance of DNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissue compared to DNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue? FAQ ID - 3618 View How is the quality of proteins purified from PAXgene Tissue fixed and stabilized tissue? FAQ ID - 3619 View Can the RNeasy kits be used to extract RNA from saliva collected using the Oragene collection kit? FAQ ID - 3623 View How do I analyze codon 61 mutations with the RAS extension kit (cat. no. 971590)? FAQ ID - 3622 View Are QR codes recognized by QIAsymphony? FAQ ID - 3625 View Do I need to use a blood collection set with the PAXgene Blood ccfDNA Tube? FAQ ID - 3626 View What is the reagent in the PAXgene Blood ccfDNA Tube? FAQ ID - 3632 View Does the insert sequence provided in the specification sheet correspond to the target sequence or to anti-sense sequence? FAQ ID - 3627 View What is the shelf life of unused PAXgene Blood ccfDNA Tubes? FAQ ID - 3633 View How should unused PAXgene Blood ccfDNA Tubes be stored? FAQ ID - 3634 View Are there special considerations for phlebotomy when using the PAXgene Blood ccfDNA Tube (blood collection set, discard tube, positioning of the tube, issues with short draws, etc.)? FAQ ID - 3635 View Are PAXgene Blood ccfDNA Tubes sterile? FAQ ID - 3636 View What is the expected ccfDNA yield from plasma generated from 10 ml of blood? FAQ ID - 3637 View Can I use plasma from underfilled PAXgene Blood ccfDNA Tubes? FAQ ID - 3638 View How long can whole blood stabilized in PAXgene Blood ccfDNA Tubes be stored at room temperature? FAQ ID - 3639 View Can whole blood in PAXgene Blood ccfDNA Tubes be stored at temperatures above 25°C? FAQ ID - 3640 View Can whole blood in PAXgene Blood ccfDNA Tubes be stored at temperatures below 15°C? FAQ ID - 3641 View Can whole blood be frozen in PAXgene Blood ccfDNA Tubes? FAQ ID - 3642 View Is double centrifugation required for plasma processing? FAQ ID - 3643 View How long and at which temperature can plasma generated from whole blood in PAXgene Blood ccfDNA Tubes be stored? FAQ ID - 3644 View What should I do if cryoprecipitates form while thawing a frozen plasma sample? FAQ ID - 3646 View Which plasma volumes can I process with the QIAsymphony PAXgene Blood ccfDNA protocols? FAQ ID - 3648 View How many extractions can I perform with a QIAsymphony PAXgene Blood ccfDNA Kit, using the 2.4 ml or the 4.8 ml protocols? FAQ ID - 3649 View How long after first use can a reagent cartridge be stored for further use? FAQ ID - 3650 View For what type of sample material is the QIAsymphony PAXgene Blood ccfDNA Kit intended? FAQ ID - 3652 View Which tubes are recommended for the QIAsymphony sample rack FAQ ID - 3653 View Which protocol line should I use to process plasma samples? FAQ ID - 3654 View Does the QIAsymphony PAXgene Blood ccfDNA Kit also allow eluting ccfDNA in less than 60 µl? FAQ ID - 3656 View How should eluates produced with the PAXgene Blood ccfDNA System be stored? FAQ ID - 3657 View Which downstream applications have been tested with ccfDNA purified from whole blood collected and processed with the PAXgene Blood ccfDNA System? FAQ ID - 3658 View What is the shelf life of the QIAsymphony PAXgene Blood ccfDNA Kit? FAQ ID - 3651 View Which elution volumes are available with the QIAsymphony PAXgene Blood ccfDNA Kit? FAQ ID - 3655 View Why is a blood collection set required to collect blood into the PAXgene Blood ccfDNA Tube? FAQ ID - 3628 View Can reagent from the PAXgene Blood ccfDNA Tube flow back into the patient’s arm? FAQ ID - 3629 View Can I use a different brand blood collection set with the PAXgene Blood ccfDNA Tube? FAQ ID - 3630 View What is the blood draw volume of the PAXgene Blood ccfDNA Tube FAQ ID - 3631 View What is the difference between RT² Profiler PCR Array and RT² Profiler PCR Array PLUS version? FAQ ID - 3685 View Where can I find the gene list for a specific RT² Profiler PCR Array? FAQ ID - 3684 View Is it possible to store nucleic acids extracted using the SpeedXtract Virus Kit? FAQ ID - 3680 View Total RNA from what sample types are compatible with the QIAseq miRNA Library Kit? FAQ ID - 3660 View Can miRNA sequencing libraries be prepared from total RNA isolated from heparinized plasma samples? FAQ ID - 3661 View Are any components of the QIAseq miRNA Library Kit interchangeable with components from other QIAGEN kits? FAQ ID - 3662 View Can I still perform sequencing if my miRNA sequencing library concentrations are too low to obtain a 4 nM library? FAQ ID - 3666 View What method is used to eliminate adapter dimers from the QIAseq miRNA library? FAQ ID - 3667 View Does a QIAseq miRNA library include hY4 Y RNA? FAQ ID - 3670 View What is the sequence of the QIAseq miRNA NGS 3’ Adapter? FAQ ID - 3671 View Do the covered regions in the BED file include the primers? FAQ ID - 3689 View What do the GTIN, UDI and REF abbreviations mean on the label of any QIAGEN box? FAQ ID - 3697 View Do eluates generated with the PAXgene Blood miRNA Kit contain residual genomic DNA? FAQ ID - 3637 View Do you have a protocol for high-throughput purification of plasmid DNA using the BioRobot Universal System? FAQ ID -1191 View Is the surface tension of the crystallization supports of the EasyXtal 15-Well Tools similar to that of a siliconized cover slide? FAQ ID -1107 View Is the pH indicated in the formulation sheet for Screening Suite buffers the final pH? FAQ ID -1123 View What are the applications the EasyXtal 15-Well Tool can be used for? FAQ ID -1111 View Is the EasyXtal Tool compatible with liquid-handling systems and visualization robots? FAQ ID -1118 View Is it possible to collect intact mitochondria before or after the density gradient centrifugation step of the Qproteome Mitochondria Isolation protocol? FAQ ID -1187 View What cell lines have been tested with the Fastlane Cell cDNA Kit? FAQ ID -1175 View What does 'QIAGEN Certified Solution' for Protein Crystallization mean? FAQ ID -1121 View How many protein crystallization conditions are available at QIAGEN? FAQ ID -1119 View How much detection reagent do you generally recommend for LiquiChip Assays? FAQ ID -1159 View How many cells can be used with the Qproteome Mitochondria Isolation Kit, and how much mitochondrial protein can be expected? FAQ ID -1189 View How many drops can be set up on a protein crystallization support? FAQ ID -1110 View How many LiquiChip beads are recommended for an xMAP experiment? FAQ ID -1161 View How much solution can I add to an EasyXtal 15-Well Tool reservoir well? FAQ ID -1114 View In which formats are crystallization condition Screening Suites available? FAQ ID -1127 View If I cannot find a QuantiTect Primer Assay for my target gene in rat, can I use the assays for the orthologous genes in mouse and human? FAQ ID -1145 View If a QuantiTect Primer Assay has more than one version number, does this mean that earlier assay versions may not work? FAQ ID -1136 View How sensitive is the LiquiChip Reader? FAQ ID -1157 View How much time is needed for a LiquiChip assay point measurement? FAQ ID -1162 View Why are there various different QuantiTect Primer Assays for my gene (previous versions, transcript variants)? FAQ ID -1133 View What is the delivery time for QuantiTect Primer Assays? FAQ ID -1142 View Where can I find the composition tables for solutions used in individual Crystallization Screening Suites? FAQ ID -1124 View What kind of strategy for initial screening of protein crystallization conditions do you recommend? FAQ ID -1120 View When searching for a QuantiTect Primer Assay for my gene of interest, why do I only find the assay for human but not for rat? FAQ ID -1139 View What type of magnet do you recommend for handling the magnetic beads in the Qproteome Plasma Membrane Protein protocol? FAQ ID -1193 View What starting material can be used with the Qproteome Mitochondria Isolation Kit? FAQ ID -1184 View Can the EZ1 DNA Investigator Tip Dance protocol cope with any solid matrix remaining in the sample tube? FAQ ID -1153 View Are EasyXtal 15-Well Tools stackable? FAQ ID -1116 View Why can I not find the QuantiTect Primer Assay in GeneGlobe that I had previously ordered from QIAGEN? FAQ ID -1138 View Which QuantiTect Primer Assay should I choose for my gene of interest? FAQ ID -1135 View Why does the QuantiTect Primer Assay for my gene of interest have only one version number? FAQ ID -1134 View Which chemical products do you use to prepare screening solutions for protein crystallization? FAQ ID -1122 View Will proteins eluted in the Qproteome Plasma Membrane Protein protocol be modified, e.g. with the ligand? FAQ ID -1195 View Will the ligand in the Qproteome Plasma Membrane Protein protocol be specific for plasma membrane exclusively? FAQ ID -1198 View Will integral, peripheral as well as lipid-anchored plasma membrane proteins be isolated with the Qproteome Plasma Membrane Protein Kit? FAQ ID -1199 View Why is there no QuantiTect Primer Assay for my gene of interest? FAQ ID -1140 View What is the EasyXtal 15-Well Tool made of? FAQ ID -1106 View What is the dynamic range of detection of the LiquiChip Reader? FAQ ID -1158 View Can the nuclei and cell debris fraction generated in the QProteome Mitochondria isolation procedure be used for further fractionation? FAQ ID -1185 View Can the EZ1 DNA Forensic Card be used with the EZ1 DNA Investigator Kit? FAQ ID -1151 View Can the EasyXtal 15-Well Tools be reused? FAQ ID -1117 View Can the Qproteome Plasma Membrane Protein Kit be used with suspension cells? FAQ ID -1194 View Can your Tag antibody recognize rat MAPK1 also? -100 View Can xMAP kits from other Luminex partners be used on the LiquiChip System purchased from QIAGEN? FAQ ID -1156 View Can the remaining fractions obtained in the Qproteome Plasma Membrane Protein procedure be used in downstream assays? FAQ ID -1197 View Can the Spin Columns of the Qproteome Albumin/IgG Depletion Kit be reused? FAQ ID -1173 View Are scoring sheets available for each crystallization condition Screening Suite? FAQ ID -1125 View Can mitochondria separated with the Qproteome Mitochondria Isolation Kit be used for downstream DNA isolation? FAQ ID -1188 View Are protein modifications such as glycosylation problematic when using the Qproteome Plasma Membrane Protein Kit? FAQ ID -1196 View Can acetone be used for precipitation of protein from Buffer RLT lysates generated with RNeasy Kits? FAQ ID -1164 View Can I increase the annealing temperature recommended for QuantiTect Primer Assays used with the QuantiTect SYBR Green PCR Kits? FAQ ID -1146 View How are NeXtal deep-well blocks sealed? Can they be resealed? FAQ ID -1128 View Does the EasyXtal Tool conform to an SBS format? FAQ ID -1115 View Do you have WGA protocols for starting materials not mentioned in the REPLI-g Handbooks? FAQ ID -1160 View How easy is it to scoop up a protein crystal with the lip around the crystallization supports of the EasyXtal 15-Well Tools? FAQ ID -1113 View Do you have a protocol for the purification of 6xHis-tagged proteins using BioSprint? FAQ ID -1167 View How did you determine that your AllStar Negative Control siRNA binds to RISC? FAQ ID -1180 View How is the quality of the crystallization condition Screening Suites guaranteed? FAQ ID -1126 View How do I use EasyXtal Crystallization Tools? FAQ ID -1105 View How does the sealing efficiency of the crystallization supports of the EasyXtal 15-Well Tool compare to grease? FAQ ID -1109 View Do you have a kit for protein isolation from mitochondria prepared with your Qproteome Mitochondria Isolation Kit? FAQ ID -1190 View Do you have a kit for the isolation of RNA from formalin-fixed, paraffin-embedded samples? FAQ ID -1174 View Do I need an ultracentrifuge for the density gradient centrifugation step in the Qproteome Mitochondria Isolation protocol? FAQ ID -1186 View Do protein crystals stick to the walls of cavities when using the DropGuard crystallization supports? FAQ ID -1108 View Do you have a protocol for evaluating pipetting accuracy of the BioRobot EZ1? FAQ ID -1131 View Do you have a protocol for purification of DNA from stool samples for pathogen detection using BioSprint Instruments? FAQ ID -1165 View Do you have a protocol for purification of DNA from stool samples using BioSprint Instruments? FAQ ID -1166 View Do you have a protocol for evaluating temperature accuracy of the BioRobot EZ1? FAQ ID -1168 View Which competent E. coli cells should I use to amplify the SureSilencing shRNA Plasmids? FAQ ID -2890 View Which analyses can be performed with the PyroMark Q96 ID software version 1.0? FAQ ID -2845 View Does the same U1 promoter in the SureSilencing shRNA Plasmids express shRNA in human, mouse, and rat cell lines? FAQ ID -2887 View How can I use the SureSilencing shRNA Plasmids for stable transfection and knock down in a cell line that is already resistant to both neomycin and puromycin? FAQ ID -2895 View Can I use my own siRNA design? FAQ ID -2897 View Can I order the nucleotides from PyroMark Gold Reagents separately? FAQ ID -2827 View Can I reclone the insert sequences from the SureSilencing shRNA Plasmids into a viral-based vector? FAQ ID -2896 View Can I directly transfect SureSilencing shRNA Plasmids into my cells? FAQ ID -2899 View Do any of the SureSilencing shRNA Plasmids contain inducible promoters? FAQ ID -2888 View Can PyroMark Gold reagents be vortexed? FAQ ID -2844 View What are the features of the new PyroMark Q96 ID software 2.5? FAQ ID -2867 View What cell line does QIAGEN use for quality control testing of the SureSilencing shRNA Plasmids? FAQ ID -2885 View Should I use the SureSilencing shRNA Plasmids with the neomycin-resistance marker, the puromycin-resistance marker, or the GFP reporter gene? FAQ ID -2882 View What else do I need to complete an experiment using the SureSilencing shRNA Plasmids? FAQ ID -2889 View What cell lines can I use with the SureSilencing shRNA Plasmids? FAQ ID -2891 View How long should I wait after transfection before enriching my transfected cells, validating knock down, and looking for phenotypic changes? FAQ ID -2893 View Is the expression level of miRNA precursors lower compared to that of mature miRNAs? FAQ ID -2807 View How many times can the CDTs, NDTs, and RDTs be used? FAQ ID -2865 View What is the PyroMark Q96 Data Converter? FAQ ID -2868 View What is the pyrosequencing data exchange tool for? FAQ ID -2864 View What is the composition of Buffer APP? FAQ ID -2804 View What is the composition of Buffer FRN? FAQ ID -2802 View What is the composition of Buffer APL? FAQ ID -2803 View Which analyses can be performed with the PyroMark Q96 MD software? FAQ ID -2866 View Which PyroMark Gold Q96 Reagent should be used for which instrument and application? FAQ ID -2836 View What is the SureSilencing shRNA Plasmids guarantee? FAQ ID -2898 View What primers may I use to sequence the shRNA cassette within the SureSilencing shRNA Plasmids? FAQ ID -2886 View Does the PyroMark CpG LINE assay target mouse transposons as well? -1 View How do I find the SureSilencing shRNA Plasmids for my gene of interest? FAQ ID -2883 View How are the SureSilencing shRNA Plasmids shipped? FAQ ID -2884 View What is the concentration of SureENTRY Transduction Reagent? FAQ ID - 3708 View Is it possible to get a Custom RT2 Profiler PCR Array, cat. # 330131, with primer assays targeting transcripts of different species, e.g. assays targeting transcripts from mouse and assays targeting transcripts from human, on one plate? FAQ ID - 3710 View Is it possible to purchase Buffer VXL which is part of the QIAamp cador Pathogen Mini Kit, (Cat No.: 54104, 54106) separately? FAQ ID - 3712 View What sample tubes can I use on my QIAsymphony SP system? FAQ ID - 3713 View How can I decontaminate my QIAsymphony SP / AS system? FAQ ID - 3714 View Can other QIAGEN buffers from non-UCP kits (e.g. AW1 instead of AUW1) be used with the QIAamp UCP DNA Micro Kit (cat. no. 56204)? FAQ ID - 3717 View How is the Pyromark Q48 cartridge to be cleaned? FAQ ID - 3718 View Can the disc and absorber strips for the Pyromark Q48 be reused? FAQ ID - 3720 View What is the dead volume of the cartridges in PyroMark Q48? FAQ ID - 3719 View How much DNA is needed in the PyroMark Q48? FAQ ID - 3721 View Can I use PyroMark Q48 for diagnostics? FAQ ID - 3722 View Can I transfer my assays from PyroMark Q24/Q96 to PyroMark Q48 Autoprep? FAQ ID - 3723 View Can I analyze my Next Generation Sequencing (NGS) samples on the QIAxcel for Quality control? FAQ ID - 3724 View Is an additional proteinase K digest possible with DNeasy PowerSoil Kit and DNeasy PowerLyzer Kit? FAQ ID - 3725 View Can I buy the 1 ml Bead Plates from the DNeasy UltraClean 96 Microbial Kit (384) separately? FAQ ID - 3726 View Does QIAGEN offer an anti-GFP gapmer control? FAQ ID - 3739 View Why do I observe a CT shift for the Internal Control in some samples in comparison to the CT value for the Internal Control of the dilution series of the Male Control DNA M1? FAQ ID - 3745 View How long can I store the dilutions of the Male Control DNA M1 standards at 2–8°C? FAQ ID - 3744 View Do I need to buy special kits for the QIAcube Connect? FAQ ID - 141485 View Can I program my own protocols for the QIAcube Connect? FAQ ID - 141486 View Can the QIAcube Connect rotor and/or buckets be removed for cleaning? FAQ ID - 141491 View Can purification columns from other suppliers be processed on the QIAcube Connect? FAQ ID - 141487 View How can I get software updates for the QIAcube Connect? FAQ ID - 141494 View Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube Connect? FAQ ID - 141496 View Do I need to discard partially used QIAcube Connect tip racks? FAQ ID - 141499 View How can I decontaminate the QIAcube Connect system? FAQ ID - 141500 View Can the QIAcube Connect heater/shaker be used independently from protocol runs? FAQ ID - 141502 View How can I load new protocols onto the QIAcube Connect? FAQ ID - 141501 View What dedicated QIAcube Connect kits are available? FAQ ID - 141504 View Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube Connect? FAQ ID - 1414507 View Where can I find the ordering information for QIAcube Connect accessories such as shaker rack plugs, rack labeling strips, reagent bottle racks, 30 ml reagent bottles, rotor-adapter holder, rotor adapters, sample tubes CB, sample tubes RB and spin-column adapter rings? FAQ ID - 141505 View Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube Connect? FAQ ID - 141506 View Which QIAcube Connect standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet? FAQ ID - 141508 View How often should the o-ring for the pipettor tip adapter be changed on the QIAcube Connect? FAQ ID - 141509 View Can the CB and RB sample tubes be used interchangeably? FAQ ID - 141510 View Which network settings are supported by the QIAcube Connect and Q-Base? FAQ ID - 141517 View Why can I not find the Q-Base device in the device list during the first installation? FAQ ID - 141516 View Why can’t I see my QIAcube Connect instrument in the QIAcube Connect App? FAQ ID - 1414518 View Why is the QIAcube Connect App reacting so slowly? FAQ ID - 141519 View Why are not all applications/kits/protocol files visible in the run setup (QIAcube Connect App)? FAQ ID - 1414520 View Why does the connection test fail? FAQ ID - 141522 View How can I accurately determine the amount of input material for the EpiTect Hi-C Kit? FAQ ID - 143065 View What sample source do you support? FAQ ID - 143066 View How long does it take to complete the EpiTect Hi-C protocol? FAQ ID - 143068 View How many reactions are in a kit? FAQ ID - 143067 View Can I use cells that have been previously crosslinked and frozen (e.g., for a ChIP or ChIP-seq experiment) as input material for the EpiTect Hi-C protocol? FAQ ID - 143069 View Can I substitute the endonuclease used in the Hi-C Digestion step with another endonuclease? FAQ ID - 143070 View Are there stopping points in the Hi-C workflow? FAQ ID - 143071 View With which platform can I perform my sequencing? FAQ ID - 143072 View With which read length should the EpiTect Hi-C NGS libraries be sequenced FAQ ID - 143073 View How do I know if my Hi-C sample is a good candidate for deep sequencing? FAQ ID - 143074 View What kind of analysis does the EpiTect Hi-C Data Analysis Portal perform? FAQ ID - 143076 View Can I use my own pipeline to analyze results from my EpiTect Hi-C experiments? FAQ ID - 143078 View How critical is the time of the temperature change from 60°C to 80°C? FAQ ID - 143768 View Is it possible to use Lyse&Spin baskets? FAQ ID - 143762 View Why is DTT used only for semen samples? FAQ ID - 143761 View Can the Repli-g Advanced DNA Single Cell Kit also be used for bacterial cells? FAQ ID - 143079 View Can the lysis volume be reduced for higher sensitivity? FAQ ID - 143763 View What if only one heater shaker is available? FAQ ID - 143764 View Why is there no male DNA obtained from sexual assault samples? FAQ ID - 143767 View If 400 µl are used as lysis volume, how do I concentrate the lysate? (Otherwise the DNA would be too diluted.) FAQ ID - 143766 View Why is the QIAamp Viral RNA Mini Kit (50) currently unavailable? FAQ ID -147395 View What RNAs has QIAseq FastSelect been designed to remove? FAQ ID -147910 View How does QIAseq FastSelect work? FAQ ID -147907 View Is QIAseq FastSelect truly as easy as combining a reagent with RNA and ramping down the temperature? FAQ ID -147909 View Will QIAseq FastSelect work in other species? FAQ ID -147911 View What library prep kits has QIAseq FastSelect been tested with? FAQ ID -147912 View I am using an RNA library prep kit that is not listed in the QIAseq FastSelect handbook. Will QIAseq FastSelect work with my kit? FAQ ID -147913 View What RNA range has the QIAseq FastSelect been tested with? FAQ ID -147914 View Can QIAseq FastSelect Plant or QIAseq FastSelect Yeast be combined with FastSelect 5S/16S/23S? FAQ ID -147915 View Is it possible to test the efficiency of FastSelect rRNA removal by using a Bioanalyzer, etc.? FAQ ID -147916 View Is plasmid DNA purified with QIAGEN Plasmid Purification Kits suitable for in vitro transcription? FAQ ID -1 View How should I quantify RNA isolated with RNeasy Kits? FAQ ID -32 View When is chloramphenicol amplification of plasmids performed? FAQ ID -3 View What are the expected DNA yields from different tissues using the QIAamp DNA Mini Kit? FAQ ID -45 View What are the most commonly used protease inhibitors? FAQ ID -53 View How should I propagate pQE expression plasmids? FAQ ID -58 View How can I increase the amount of soluble recombinant protein in E. coli expression? FAQ ID -64 View How can I remove imidazole from a protein sample? FAQ ID -91 View Why does my isolated RNA have a low OD 260/280 ratio? FAQ ID -97 View How can I avoid little or no RNA yields when using an RNeasy Kit? FAQ ID -28 View Do I need to use an RNase inhibitor in my RT reaction? FAQ ID -119 View Can I use QIAprep Miniprep kits for low-copy plasmids and cosmids? FAQ ID -127 View Can I use the QIAquick PCR Purification Kit for restriction enzyme cleanup? FAQ ID -130 View How does imidazole affect my quantitation of protein? FAQ ID -132 View Can a QIAquick Gel Extraction Kit be used to obtain RNA from a formaldehyde gel? FAQ ID -133 View What is the difference between disruption and homogenization in the RNeasy System? FAQ ID -139 View What is the size of genomic DNA that is obtained with QIAGEN Genomic-tips? FAQ ID -142 View Should I use Ni-NTA Agarose in column or batch format for purification of 6xHis-tagged proteins? FAQ ID -147 View What is the genotype of the EZ Competent Cells? FAQ ID -157 View What are the restriction sites of the pDrive Vector in the QIAGEN PCR Cloning Kit? FAQ ID -160 View What are the molecular weights of proteins in the 6xHis Protein Ladder? FAQ ID -169 View How can I optimize the transfection of oligos and large plasmids using PolyFect Transfection Reagent? FAQ ID -176 View How can I improve recoveries when using the QIAquick Kits? FAQ ID -180 View How can I improve transfection efficiency using Effectene Transfection Reagent? FAQ ID -181 View What is the principle behind Effectene Transfection Reagent? FAQ ID -184 View What is the composition of Buffer EB? FAQ ID -199 View Is it possible to modify the transduction protocol for Cignal Lenti Reporter Assays to save some pipetting steps? FAQ ID - 3709 View Can I store the QIAseq Beads of the QIAseq Targeted DNA Panel at -20°C? FAQ ID - 3711 View What is the difference between the QIAamp UCP DNA Micro Kit (cat. no. 56204) and RNeasy UCP Kits (cat. no. 73934) and non-UCP QIAGEN purification kits? FAQ ID - 3715 View Can I use carrier RNA with the RNeasy UCP Micro Kit (cat. no. 73934)? FAQ ID - 3716 View How can I check for purity of RNA isolated using RNeasy Kits? FAQ ID -1023 View What is the recipe for 1x PBS solution? FAQ ID -1030 View Where can I find a protocol for cleanup of already purified plasmid DNA? FAQ ID -1031 View How do I perform an Acetone Precipitation for concentrating and desalting protein samples? FAQ ID -1035 View What is the difference between Buffers RLT and RLT Plus? FAQ ID -1043 View What is the largest PCR amplicon that can be amplified with the HotStar HiFidelity Polymerase Kit? FAQ ID -1047 View Are the CoralLoad dyes in the HotStarTaq Plus PCR Buffer visible when loading small amounts of PCR product onto a gel? FAQ ID -1051 View Do I have to use CoralLoad Gel loading dye when using your HotStarTaq Plus DNA Polymerase? FAQ ID -1052 View How can I increase DNA concentration using QIAprecipitators of the HiSpeed Plasmid Kits? FAQ ID -1061 View What should I use as a standard for absolute quantification in real-time PCR? FAQ ID -1085 View What does transcript variant mean when searching for specific QuantiTect Primer Assays? FAQ ID -1137 View What is a QuantiTect Primer Assay? FAQ ID -1141 View Do QuantiTect Primer Assays contain SYBR Green dye? FAQ ID -1143 View What is the smallest and the largest protein that you tested for expression with the EasyXpress Insect Kit II? FAQ ID -1218 View What are the new features of the EasyXpress Insect Kit II compared to the original Protein Synthesis Insect kit? FAQ ID -1220 View What are your recommendations for PCR template preparation for use with the EasyXpress Insect Kit II? FAQ ID -1221 View Do you have a protocol for transfection of suspension cell lines (Jurkat and K562) with siRNA? FAQ ID -1250 View Do you have a protocol for transfection of macrophage cell lines (J774.A1 and RAW 264.7) with siRNA? FAQ ID -1251 View What is the pH value of QIAGEN's Nuclease-Free Water? FAQ ID -1290 View Why is there DNA in the no-template control reaction when using the standard REPLI-g procedure, but not when using the UltraFast procedure? FAQ ID -1327 View Will the REPLI-g UltraFast Mini Kit reaction always yield 10 µg DNA? FAQ ID -1328 View Can the bacterial pellet in the QuickLyse Miniprep procedure be resuspended by pipetting up and down or shortening the vortexing time? FAQ ID -1352 View How can I increase the DNA yield from low-copy plasmids using the QuickLyse Miniprep Kit? FAQ ID -1354 View What is the composition of elution buffer QLE in the QuickLyse Miniprep Kit? FAQ ID -1356 View How do I resuspend my FlexiPlate siRNAs? FAQ ID -1360 View Are there any specific cleaning solutions recommended for the QIAcube? FAQ ID -1416 View Why is a 2-step (and not a 3-step) cycling protocol recommended for QuantiFast SYBR Green PCR Kits? FAQ ID -1450 View Can tissue homogenized in Buffer RLT Plus instead of Buffer RLT be used with the AllPrep DNA/RNA/Protein Mini Kit? FAQ ID -1577 View What can be done if the protein pellet does not solubilize completely using the AllPrep DNA/RNA/Protein Mini Kit? FAQ ID -1580 View What is the binding capacity of the Ni-NTA Superflow Cartridges? FAQ ID -1603 View How comparable is CoralLoad gel loading dye contained in various QIAGEN PCR Kits to Sigma Red? FAQ ID -1644 View Do I need to resuspend FlexiTube siRNAs in siRNA Resuspension buffer? FAQ ID -1659 View Are FlexiTube siRNA and FlexiTube GeneSolution also available for rat siRNAs? FAQ ID -1664 View What is the composition of Buffer PE? FAQ ID -1691 View What is the upper length of PCR products that can be amplified with TopTaq DNA Polymerase and Master Mix Kits? FAQ ID -1738 View What is the minimum sample volume required for the QIAxcel System and QIAxcel Advanced? FAQ ID -1852 View What are the dimensions and weight of the QIAsymphony SP System? FAQ ID -1901 View Are there any specific cleaning solutions recommended for the QIAsymphony SP? FAQ ID -1913 View How much sample can be used with the RNeasy Plus 96 Kit? FAQ ID -1992 View What is the composition of Buffer P2? FAQ ID -203 View A white precipitate has formed in my 10x RT buffer. Is it still ok to use? FAQ ID -216 View For how long are BioMag Particles stable? FAQ ID -262 View I used to buy BioMag particles from a company by another name? Are your BioMag particles the same thing? FAQ ID -263 View Can I clean up my DNase treated RNA samples using RNeasy columns? FAQ ID -286 View How can one determine the optimal annealing temperature for a specific PCR assay? FAQ ID -288 View What is the composition of the QIAGEN Multiplex PCR Buffer? FAQ ID -289 View Can I use HEPES buffer instead of phosphate in my Ni-NTA column? FAQ ID -291 View Can I use a centrifuge instead of vacuum when using the QIAquick 96 PCR Purification Kit? FAQ ID -293 View What are the ideal and the largest PCR amplicon sizes when using the EpiTect MSP Kit? FAQ ID -2004 View What is elution Buffer ATE of the QIAsymphony DNA Investigator Kit? FAQ ID -2029 View How can a C-terminal 6xHis-tagged QIAgene be expressed? FAQ ID -2041 View Why is there more than one QIAgene for my gene of interest? FAQ ID -2044 View Is a passive internal reference dye, like ROX, required on the Rotor-Gene Q to obtain reproducible results? FAQ ID -2079 View Does the Rotor-Gene Q software have the capacity to export data? FAQ ID -2089 View Why is a 2-step (and not a 3-step) cycling protocol recommended for Rotor-Gene SYBR Green Kits? FAQ ID -2122 View Does carrier RNA have to be added using the QIAsymphony Virus Blood 200 Protocol? FAQ ID -2163 View Can I use the RNeasy Microarray Tissue Mini Kit for all tissue types? FAQ ID -2190 View How is the RNeasy Microarray Tissue Mini Kit different from the classic RNeasy Mini Kit? FAQ ID -2192 View Why is EvaGreen instead of SYBR Green used as fluorescent dye in the Type-it HRM PCR Kit? FAQ ID -2196 View Why do I need normalization using Rotor-Gene ScreenClust HRM Software? FAQ ID -2198 View What is the difference between probability and typicality in the Rotor-Gene ScreenClust HRM Software? FAQ ID -2203 View What buffer conditions give the best resolution for agarose gel electrophoresis? FAQ ID -2256 View How much DNA should be loaded per well of an agarose gel? FAQ ID -2259 View Why do I have wavy DNA bands on my agarose gel? FAQ ID -2260 View What is the "seed sequence" of a miRNA? FAQ ID -2262 View Are you able to supply a Certificate of Analysis for the KRAS and EGFR PCR kits? FAQ ID -2301 View Which Rotor-Gene Q rotor should be used with the EGFR RGQ PCR kit? FAQ ID -2315 View How is the RNeasy Plus Universal Kit different from the RNeasy Plus Kit? FAQ ID -2340 View How does the gDNA eliminator solution of the RNeasy Plus Universal Kit work? FAQ ID -2341 View How long can I store my purified DNA/RNA isolated with the AllPrep DNA/RNA FFPE kit? FAQ ID -2350 View Do you have any information or guidelines regarding the choice of reference genes for real-time PCR? FAQ ID -2371 View Can I buy QIAquick and MinElute columns separately? FAQ ID -2460 View What is the PAXgene 96 filter plate? FAQ ID -2486 View Is RNA from red blood cells also isolated when RNA is extracted from a PAXgene Bone Marrow RNA Tube? FAQ ID -2509 View Is the 260/230 nm absorbance ratio specified for RNA derived from the PAXgene Bone Marrow RNA System? FAQ ID -2514 View How long is the fixation time? FAQ ID -2519 View What is the nature of the heterologous Internal Amplification Control and Positive Control in the mericon Assay kits? FAQ ID -2543 View Are there any accessories required for sample disruption with the TissueLyser LT? FAQ ID -2551 View Why do the Internal Control templates for extraction (Internal Control DNA or RNA [High conc.]) have a 10x higher concentration than the IC templates provided with the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit? FAQ ID -2603 View During data analysis, how should the threshold be set in the Yellow channel on the Rotor-Gene Q cycler to analyze the Internal Control from the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit FAQ ID -2607 View What is the SBTengine® software? FAQ ID -2628 View How do I create a workspace that is free of DNA contamination, prior to carrying out a qPCR experiment? FAQ ID -2654 View What is the recommended solution in which to store RNA samples that will be used as templates for cDNA synthesis? FAQ ID -2659 View How can I avoid or remove genomic DNA contamination from the total RNA preparation? FAQ ID -2662 View What are the differences between one-step and two-step RT--PCR? FAQ ID -2666 View What is qPCR? FAQ ID -2668 View What are the common primer and probe chemistries utilized for qPCR assays? FAQ ID -2669 View What is the purpose of the ROX and fluorescein dyes, also known as passive reference dyes, in qPCR master mixes? FAQ ID -2671 View What negative controls are typically included in qPCR and/or qRT-PCR experiments? FAQ ID -2672 View What positive controls are typically included in qPCR and/or qRT-PCR experiments? FAQ ID -2673 View What are the guidelines for choosing a housekeeping gene for normalizing qPCR results? FAQ ID -2674 View Why is 18S ribosomal RNA (rRNA) used as a housekeeping gene to normalize sample-to-sample, systematic variation in qPCR assays? FAQ ID -2675 View How does HotStart PCR help minimize nonspecific amplification events? FAQ ID -2676 View What is a dissociation curve, and why is it important to run a dissociation curve, following qPCR using SYBR Green chemistry? FAQ ID -2678 View What is the delta Rn value? FAQ ID -2681 View What is the threshold cycle or Ct value? FAQ ID -2682 View Why are my qPCR Ct values too low (< 12) in my qRT-PCR Assay? FAQ ID -2684 View Why are my qPCR Ct values too high (> 35 or not detectable) in my qRT-PCR assay? FAQ ID -2685 View Why is my no template control (NTC) real-time Ct value < 35 cycles in my qPCR Assay? FAQ ID -2686 View Why do my qPCR amplification curves or plots decrease in fluorescence intensity after the saturation phase? FAQ ID -2689 View Why do I see multiple high-intensity peaks in my qPCR dissociation curve at temperatures less than 70 ºC? FAQ ID -2690 View What is the standard curve method for qPCR assay data analysis? How is the standard curve method for qPCR assay data analysis performed? FAQ ID -2691 View What is the difference between Absolute Quantification and Relative Quantification in qPCR, using the standard curve approach? FAQ ID -2692 View What is the comparative or ??Ct method for qPCR assay data analysis? How is the comparative or ??Ct method for qPCR assay data analysis performed? FAQ ID -2693 View How do I determine the amplification efficiency of my qPCR assay? FAQ ID -2694 View How do I determine the linear dynamic range of my qPCR or qRT-PCR assay? FAQ ID -2696 View What is the best approach for determining where to set the CT threshold when you have >15 samples? Is it best to go through all of them, looking for a range of best fit, and then just choose one value that fits all of them? FAQ ID -2705 View What do I need to complete a RT² qPCR Primer Assay? FAQ ID -2707 View How can I prevent the evaporation of reaction volume from the wells in the miScript miRNA PCR array? FAQ ID -2729 View What is the minimum amount of genomic DNA required for analysis using EpiTect Methyl qPCR Arrays? FAQ ID -2739 View How do you design your EpiTect ChIP qPCR Assay primers? Do they work with SB GR? FAQ ID -2749 View How can I identify the binding sites of my transcription factor using ChIP? FAQ ID -2753 View At what point can I stop and freeze my samples when using ChIP? FAQ ID -2754 View What is RNA interference (RNAi)? FAQ ID -2755 View What are the key factors for success in an RNAi experiment? FAQ ID -2758 View What criteria should one use in choosing between siRNA versus shRNA for their studies? FAQ ID -2771 View How does stable transfection of a shRNA-encoding plasmid work? FAQ ID -2779 View What are the SureSilencing shRNA Plasmids? FAQ ID -2784 View What is the composition of Buffer S3? FAQ ID -2788 View What is the composition of Buffer ETR? FAQ ID -2789 View What is the composition of Buffer BB? FAQ ID -2790 View What is the composition of Buffer RLT plus? FAQ ID -2794 View What is the composition of Buffer RLC? FAQ ID -2795 View What is the composition of Buffer RPE? FAQ ID -2797 View What is the composition of Buffer RWT? FAQ ID -2798 View What is the composition of gDNA Eliminator Solution? FAQ ID -2799 View What is the composition of Buffer RDD? FAQ ID -2800 View What is the composition of Buffer PKD? FAQ ID -2801 View What is the composition of Buffer ALO? FAQ ID -2805 View What is the composition of RBC Lysis Solution? FAQ ID -2808 View What is the composition of Cell Lysis Solution? FAQ ID -2809 View What is the composition of Protein Precipitation Solution? FAQ ID -2810 View What is the composition of Cell Suspension Solution? FAQ ID -2811 View What is the composition and concentration of Glycogen Solution in Gentra Puregene Kits? FAQ ID -2812 View What is the composition of DNA Hydration Solution in Gentra Puregene Kits? FAQ ID -2813 View What is the meaning of the abbreviations NDT and CDT and what are the differences? FAQ ID -2841 View What are the changes of PyroMark Assay Design Software Version 2.0 compared to 1.0? FAQ ID -2849 View Can I use fluorescence microscopy to assess the transfection efficiency of the SureSilencing shRNA Plasmids with GFP in my model cell line of interest? FAQ ID -2894 View Do tissue samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction? FAQ ID -2926 View What is the composition of Buffer G2? FAQ ID -2943 View What is the composition of Buffer B1? FAQ ID -2944 View What is the composition of Buffer B2? FAQ ID -2945 View How much DNA does a human cell contain? FAQ ID -2948 View How much RNA does a bacterial cell contain? FAQ ID -2949 View How much DNA and RNA can be expected from human blood cells? FAQ ID -2950 View What is the cellular composition of human blood? FAQ ID -2951 View How can I desalt and concentrate a protein fraction by trichloroacetic acid (TCA) precipitation? FAQ ID -2954 View Is there a risk of crosstalk from the FAM channel to the VIC channel if the residual DNA target is present in very high copy numbers? FAQ ID -2980 View Why is the final heat denaturation step so important with the QIAsymphony PAXgene Blood RNA protocol? FAQ ID -2987 View What is the sequence of the miScript Universal Primer? FAQ ID -2994 View How can I keep my centrifuge tubes endotoxin-free? FAQ ID -301 View Can Buffers N3 and P3 be used interchangeably? FAQ ID -310 View Are QIAprep and QIAquick Spin columns interchangeable? FAQ ID -311 View Can I store agarose gel slices containing DNA for gel extraction at a later point? FAQ ID -313 View How can QIAGEN Protease and Proteinase K be inactivated? FAQ ID -315 View Does the 5x OneStep RT-PCR Buffer contain BSA? FAQ ID -326 View What is the origin of replication and the plasmid copy number of the pQE vectors? FAQ ID -338 View Is QIAGEN carrier RNA sold separately? FAQ ID -351 View What is the white insoluble precipitate in my resuspended plasmid DNA pellet? FAQ ID -352 View What is the composition of PBS? FAQ ID -361 View How can I express toxic protein in E. coli? FAQ ID -373 View How can I improve DNA yields from very tough tissues using the DNeasy Blood & Tissue Kit or the QIAamp DNA Mini Kit? FAQ ID -374 View What are the dissociation constants for the Anti-His Antibodies? FAQ ID -385 View What is the average molecular weight of a siRNA, and how do I convert uM to ug values? FAQ ID -388 View Can siRNA silence bacterial genes? FAQ ID -395 View How should I reconstitute the lyophilized RNase-Free DNase Set with the RNeasy 96 kit? FAQ ID -3003 View Is it possible to use a standard processor — the kind used routinely for formalin-fixed samples — for dehydration and paraffin infiltration of PAXgene Tissue treated samples? FAQ ID -3032 View Can I use Tungsten Carbide beads for RNA extraction protocols using QIAGEN Kits? FAQ ID -3053 View Where can I find the ordering information for QIAcube accessories such as Shaker Rack plugs, Rack labeling strips, Reagent bottle rack, 30mL Reagent bottles, Rotor Adapter holder, Rotor adapters, Sample tubes CB, Sample tubes RB and Spin column adapter rings? FAQ ID -3054 View What is the part number for O-rings for QIAsymphony SP and AS instruments? How often should the O-rings be replaced? FAQ ID -3055 View What is the thermal output for a BioRobot MDx and BioRobot Universal? FAQ ID -3056 View What is the thermal output for a QIAsymphony SP and AS instrument? FAQ ID -3057 View How precise is the QIAgility? FAQ ID -3063 View What are the advantages and disadvantages of turning on the tip “re-use” feature on the QIAgility? FAQ ID -3064 View How long does it take to set up a 96-well plate PCR reaction on the QIAgility? FAQ ID -3066 View Can I use a bar code scanner or reader on the QIAgility? FAQ ID -3067 View How can I find the run parameters for a BioSprint protocol that I am running? FAQ ID -3069 View Can BioSprint 96 be connected to LIMS? FAQ ID -3070 View What is the expected level of endotoxins in plasmid DNA purified with QIAprep spin miniprep kit? FAQ ID -3081 View What is the expected level of endotoxins in plasmid DNA purified with QIAGEN Plasmid kits, QIAfilter Plasmid kits and HiSpeed Plasmid purification kits? FAQ ID -3082 View What is the concentration of the Gentra RNase A that is used with the Autopure and Puregene protocols? FAQ ID -3083 View What is the concentration of the different Proteinase K solutions sold by QIAGEN? FAQ ID -3084 View Where can I access Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit? FAQ ID -3085 View What tubes can be used with TissueLyser Adapter Set 2 x 96 (cat. no. 69984)? FAQ ID -3086 View How to upload a Flexiplate on QIAGEN Webpage? FAQ ID -3103 View Can we do modification XXXXX for custom siRNA that is not on the web page? FAQ ID -3107 View What is the stability of siRNA lyophilized and resuspended? FAQ ID -3109 View Do you offer FlexiTube or FlexiPlate siRNAs for rat? FAQ ID -3114 View Is it possible to extract RNA from frozen blood? FAQ ID -3119 View Is it possible to extract RNA from RNAprotect-stabilized-blood? FAQ ID -3120 View What's the pulsed-field gel electrophoresis (PFGE) conditions of the gel picture showing on your website? http://www.qiagen.com/Search/MagAttract-HMW-DNA-Kit#productdetails -48 View Can Dithiothreitol (DTT) be used instead of BME in Buffer RLT Plus of RNeasy Plus Mini Kit? FAQ ID -3125 View Why does my purified DNA/RNA sometimes have a 260/280 or 260/230 ratio of more than 2? Does that mean the purity is poor? FAQ ID -3132 View How do I remove the RNA contamination from purified DNA? FAQ ID -3133 View What is the final concentration of Protease upon reconstitution in the QIAamp DNA Blood Mini? FAQ ID -3140 View What is the Spike-in-Control in the miRNeasy Serum/Plasma Kit ? FAQ ID -3169 View What is the difference between the 1 nmol FlexiTube and FlexiTube Gene Solution siRNAs? FAQ ID -3174 View What could be a reason for cloudy precipitate in PCR reaction with STR Plus chemistry after cycling protocol is completed? FAQ ID -3175 View What is needed for performing delta delta Ct relative quantitation on the Rotor-Gene Q? FAQ ID -3178 View Is REST software Windows 7 compatible? FAQ ID -3182 View Can the Rotor-Gene Q perform gradient PCR? FAQ ID -3183 View What is the expected amplicon size of the Microbial DNA qPCR Assays? FAQ ID — 3396 View What is the difference between Positive PCR Control (PPC) and Microbial DNA Positive Control? FAQ ID — 3397 View What is LLOQ? FAQ ID — 3401 View What is the difference between LLOQ and LOD? FAQ ID — 3402 View What species are detected by the Pan Bacteria 1 and Pan Bacteria 3 Assays? FAQ ID - 3427 View What is the composition of Buffer AC in the QIAamp UltraSens Virus Kit? FAQ ID - 3429 View Can the miScript II RT Kit be used to quantify plant miRNAs? FAQ ID - 3431 View What is the dissociation curve temperature of PCR products generated with the miScript Plant qPCR system? FAQ ID - 3444 View Can specific types of white blood cells be enriched from a PAXgene Blood RNA Tube prior to the RNA isolation? FAQ ID - 3467 View What is the proper disposal procedure for waste from the sample preparation? FAQ ID - 3470 View What is the expected yield from 8.5 ml of blood? FAQ ID - 3496 View What does the resuspension buffer, Buffer BG4, in the PAXgene Blood DNA kit contain? FAQ ID - 3501 View How should blood samples drawn into PAXgene Blood DNA Tubes be transported? FAQ ID - 3507 View How long can samples in PAXgene Blood DNA Tubes be stored at room temperature? FAQ ID - 3508 View What is the expected DNA yield from 2.5 ml of blood when using the QIAsymphony, QIAcube, and salting-out precipitation method? FAQ ID - 3510 View Which downstream applications have been tested with DNA purified from samples collected in PAXgene Blood DNA Tubes IVD? FAQ ID - 3512 View What does PAXgene Tissue STABILIZER contain? FAQ ID - 3513 View What is the minimum number of cells that can be efficiently processed with the QIAamp DNA Mini and QIAamp Blood Mini kits and what is the expected yield? FAQ ID - 3516 View Is the quality and size of DNA extracted with the DNeasy Blood & Tissue kit good enough to generate DNA-libraries for next generation sequencing? FAQ ID - 3517 View What is the minimum number of cells or minimum amount of tissue that can be efficiently processed with the RNeasy mini kit? FAQ ID - 3519 View Can I use the RNeasy Fibrous Tissue Mini Kit (cat. no. 74704) to extract RNA from fibrous plant material? FAQ ID - 3521 View Will the Reverse transcription control on the RT2 profiler PCR array work on any cDNA library? FAQ ID - 3534 View Microscopic/visual confirmation of successfully sorted cells can be used to optimize sorting conditions. Can stained cells be used? FAQ ID - 3559 View Is the miRNA reverse transcription control (miRTC) used in conjunction with the miScript Single Cell qPCR Kit? FAQ ID - 3573 View Is clot detection possible on the QIAsymphony? FAQ - ID 3588 View How can I determine if a tube is suitable for clot detection on QIAsymphony? FAQ ID - 3590 View How should I thaw frozen plasma samples? FAQ ID - 3645 View How do Unique Molecular Indexes (UMIs) improve quantification? FAQ ID - 3669 View Can REPLI-g WGA be used to amplify cDNA if the cDNA is dsDNA (double-stranded DNA)? FAQ ID - 3686 View I would like to stop during the library preparation procedure. When can I do that? FAQ ID - 3687 View Which regions are covered by the GR DNAseq V2 Panels (#181900)? FAQ ID - 3688 View When preparing plasma with the PAXgene Blood ccfDNA tubes, cat. # 768115, do you recommend that the first centrifugation step should be done with the brakes on or off? FAQ ID - 3690 View How do I insert and replace an absorber strip into the PyroMark Q48 Autoprep chamber? FAQ ID - 3691 View Are peak heights of Q48 comparable to other PyroMark instruments? FAQ ID - 3692 View How much space does the PyroMark Q48 Autoprep instrument need? FAQ ID - 3693 View Can unused wells in a pyrosequencing discs be used in the next run? FAQ ID - 3694 View Where can I find the certificate of calibration for the Rotor-Disc OTV Kit (catalog number 981400)? FAQ ID - 3695 View What is the minimum guaranteed shelf-life of the PAXgene Blood ccfDNA Tubes (100) (catalog no. 768115)? FAQ ID - 3696 View What methods are used during normalization of RNAseq data in the secondary GeneGlobe Data Analysis Center? FAQ ID - 3698 View How do I prepare Buffer MP? FAQ ID - 3620 View Are the QIAshredder columns included in various QIAGEN kits the same? FAQ ID - 3624 View What is the composition of buffer QBT? FAQ ID -411 View What is the composition of buffer QC? FAQ ID -412 View What is the composition of buffer QF? FAQ ID -413 View What is the composition of buffer STE? FAQ ID -415 View What is the composition of buffer TE? FAQ ID -416 View What is the composition of buffer FWB2? FAQ ID -417 View What is the composition of buffer P3? FAQ ID -418 View What is the size, charge and isoelectric point of the DHFR protein in the pQE vectors? FAQ ID -470 View Why is carrier RNA used during the isolation of gDNA from microdissected samples with the QIAamp DNA Micro Kit? FAQ ID -473 View Why are the centrifugation speeds for the QIAamp DNA Mini kit at 6000 x g? Can I spin at full speed? FAQ ID -474 View Are HEPES, MOPS and PIPES buffers compatible with QIAGEN's PhosphoProtein Purification kit? FAQ ID -482 View Can Ni-NTA resins be used to purify protein with an internal His-tag? FAQ ID -496 View How do I calculate the percentage of silencing with real-time RT-PCR for siRNA? FAQ ID -498 View Do you have a protocol for the removal of endotoxins from already purified plasmid DNA? FAQ ID -500 View Do you have stability data for genomic DNA isolated with the QIAamp DNA Blood Mini Kit? FAQ ID -518 View What is the composition of the siRNA resuspension & annealing buffer? FAQ ID -522 View Can Taq DNA Polymerase use RNA as a template, and generate false positives in "no-RT" controls? FAQ ID -523 View What is Tissue-Tek O.C.T., and what is it used for? FAQ ID -530 View Why do replicates in real-time PCR have different plateau heights? FAQ ID -539 View How can I avoid primer-dimer formation during PCR amplification? FAQ ID -544 View How can I tell if I have primer-dimers in my PCR reaction? FAQ ID -552 View How should I store cDNA produced using Omniscript Reverse Transcriptase? FAQ ID -561 View Is it possible to isolate DNA from bone marrow with the QIAamp DNA Blood Kits? FAQ ID -563 View What is the composition of Buffer ER? Is it available separately? FAQ ID -571 View What can I do when the DNA pellet prepared with QIAGEN Plasmid Kits has been overdried? FAQ ID -572 View Do I have to remove the oil from my PCR reaction before using the QIAquick or MinElute PCR Purification Kit? FAQ ID -575 View Are the columns of the QIAquick PCR Purification-, Gel Extraction-, and Nucleotide Removal Kit interchangeable? FAQ ID -577 View What size is the smallest protein that can be synthesized with the EasyXpress Protein Synthesis Kit? FAQ ID -600 View Why do I sometimes get light blue colonies when using the QIAGEN PCR Cloning Kit? FAQ ID -603 View What do you recommend for the cleanup of genomic DNA (gDNA)? FAQ ID -618 View How can I improve my RNA yield from liver sample when using RNeasy Kits? FAQ ID -623 View Why do you recommend using Triton X for the purification of 6xHis-tagged protein? -100 View What 96-well plates do you recommend for use with the LiquiChip Workstation? FAQ ID -630 View What is a QIAshredder? Is it sufficient for complete disruption and homogenization of my tissue sample? FAQ ID -631 View Why does the QIAamp DNA Mini Tissue Protocol require both ATL and AL buffer, while the Blood Protocol only uses AL? FAQ ID -633 View Will the QIAquick PCR Purification Kit remove sufficient SYBR Green from real-time PCR reactions to allow sequencing? FAQ ID -637 View What is the shelf-life of my QIAGEN Kit? FAQ ID -651 View What are the differences between MDA and DOP/PEP methods of Whole Genome Amplification? FAQ ID -665 View Can I purchase Phi29 DNA polymerase only? FAQ ID -708 View What is the average DNA concentration obtained using the DirectPrep 96 Miniprep Kit? FAQ ID -721 View I accidentally stored Buffer RDD of the RNase-Free DNase Set at°C. Will it still function? -20 View I ran my RNA out on an agarose gel and can see lots of bands similar to a ladder. Why? FAQ ID -745 View How much DNA is obtained in the average PCR reaction? FAQ ID -750 View Do you have information about the cleanup of single-stranded DNA (ssDNA) with QIAquick columns? FAQ ID -759 View How can I get a certificate for quality assurance (ISO 13485 and ISO 9001) from QIAGEN? FAQ ID -762 View What is the difference between Ni-NTA Agarose and Ni-NTA Superflow? FAQ ID -764 View What is the composition of Buffer N3? FAQ ID -767 View Are the protocols from the BioSprint software compatible with the KingFisher software and vice versa? FAQ ID -770 View How should fluorescent labeled probes be stored? FAQ ID -784 View Is it possible to use the QuantiTect Reverse Transcription Kit with bacterial RNA? FAQ ID -785 View Are Buffer PB of the QIAquick PCR Purification Kit and Buffer QG of the QIAquick Gel Extraction Kit interchangeable? FAQ ID -786 View How can I get specific information and a quote for my individual Sequencing Service needs? FAQ ID -792 View Can the QIAprep Spin Miniprep Kit be used for isolating plasmid DNA from mammalian cells? FAQ ID -795 View How can I separate PCR fragments that are small and very close in size on an agarose gel? FAQ ID -800 View Can I reuse the Ni-NTA Agarose and Ni-NTA Superflow resins? FAQ ID -802 View What Disinfectant Solution do you recommend for the BioRobot MDx? FAQ ID -809 View Is the E. coli Host Strain M15[pREP4] resistant to Zeocin? FAQ ID -842 View What annealing temperature should be used with the QuantiTect Primer Assays? FAQ ID -849 View Why is it recommended to add 1 mM IPTG for optimal protein yields using the EasyXpress Protein Synthesis Kits? FAQ ID -860 View Which QIAGEN plasmid preparation kits will contain LyseBlue Reagent? FAQ ID -865 View Do you have an in vitro translation kit allowing the incorporation of seleno-methionine for X-Ray crystallography based protein analysis? FAQ ID -879 View Do you have a protocol for the isolation of genomic DNA from frozen clotted blood? FAQ ID -900. Test View Do you have protocols for the isolation of genomic DNA from tissue using QIAGEN-tips 2500 (Mega) and 10000 (Giga)? FAQ ID -907 View Do you have a protocol for the isolation of genomic DNA from bone? FAQ ID -908 View What do you suggest to prevent degradation of RNA isolated from tissue with high amounts of RNases using RNeasy? FAQ ID -942 View Do you have a protocol for Cyanine 570, Cyanine 670, or biotin labeling of cDNA? FAQ ID -959 View Do you have a protocol for reverse transfection of adherent cells with siRNA in 384-well plates? FAQ ID -971 View Do you have a protocol for the purification of PCR products using the BioSprint System? FAQ ID -984 View Do you have a protocol for transient transfection of Hela-S3 cells using PolyFect Transfection Reagent? FAQ ID -997 View How can I analyze proteins from exosomes and other extracellular vesicles (EVs) obtained with the exoEasy Maxi Kit by SDS-PAGE? FAQ ID - 3707 View How can I increase expression of my 6xHis-tagged protein in E. coli? FAQ ID -63 View Can miRNA and other RNAs smaller than 200 nucleotides be isolated with the AllPrep DNA/mRNA Nano Kit? 148723 View What is the minimum amount of starting material that can be used with the DNA/mRNA Nano Kit? 148724 View What is the composition of buffer OW2? FAQ ID -419 View What is the composition of buffer OBB? FAQ ID -421 View Do I need to buy special kits for the QIAcube Connect MDx? 148725 View What applications are offered on the QIAcube Connect MDx instrument? 148726 View Can I program my own protocols for the QIAcube Connect MDx? 148727 View Can purification columns from other suppliers be processed on the QIAcube Connect MDx? 148728 View What are the dimensions and weight of the QIAcube Connect MDx? 148729 View Can the QIAcube rotor adapters be autoclaved? 148730 View Can the QIAcube rotor and/or buckets be removed for cleaning? 148731 View Can the QIAcube be connected to a laboratory information management system? 148732 View Are run reports and/or log files available on the QIAcube? 148733 View How can I get software updates for the QIAcube Connect MDx? 148734 View Can QIAcube Connect MDx protocol runs be interrupted, and subsequently be continued to completion? 148735 View Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube? 148736 View Do I need to discard partially used QIAcube tip racks? 148738 View How can I decontaminate the QIAcube Connect MDx system? 148739 View How can I load new protocols onto the QIAcube Connect MDx? 148740 View Can the QIAcube Connect MDx heater/shaker be used independently from protocol runs? 148741 View Can the QIAcube Connect MDx centrifuge be used independently from protocol runs? 148742 View What dedicated QIAcube Kits are available? 148743 View Can I ask QIAGEN to upgrade my current QIAcube Connect Life Science instrument to make it an MDx instrument? 148744 View Can I run life science and custom protocols on a QIAcube Connect MDx? 148745 View What are the heating/cooling rates in the QIAquant instruments? 153919 View Is a passive internal reference dye, like ROX, required on the QIAquant to obtain reproducible results? 153920 View What sample volume is suitable for use in the QIAquant? 153921 View What types of reaction vessels are required for use in the QIAquant? 153922 View Is the QIAquant equipped with a temperature gradient function? 153923 View Are the 96- and 384-sample blocks interchangeable? 153924 View Is it possible to use the touch down PCR option on QIAquant with two acquisition points in the same channel? 153925 View How many molecules can be detected in the system? 153926 View What applications can the QIAquant be used for? 153927 View Can I upgrade my QIAquant 96 2plex instrument to QIAquant 96 5plex or modify the set of color channels? 153928 View Does QIAGEN offers arrays and assays configured specifically for QIAquant instrument? 153929 View Is the software of the QIAquant compatible with 21CFR part11? 153930 View Is it possible to import a standard curve from a previous run? 153947 View Can data be analyzed while the run is proceeding? Can data be analyzed from a previous experiment while another experiment is running? 153948 View Can I use QIAquant 96 Software to analyze experiments carried out on QIAquant 384? 153950 View Can I operate QIAquant 96 instrument using touchscreen and computer simultaneously? 153951 View Is it possible to establish an internet connection with QIAquant instrument? 153952 View Can I connect more than one QIAquant instrument to the same computer? 153953 View What is the algorithm behind data normalization in the QIAquant Software? 153954 View Is it possible to edit the sample sheet after the run in the QIAquant Software? 153955 View What are the LIMS capacity of the software? 153949 View Does the QIAquant Software have the capacity to export data? Which data formats can be used for the export? 153946 View I received a kit containing the MinElute columns; however, they were left out for a while and not stored at 2–8°C upon receipt. Can I still use them? FAQ ID - 3560 View Is the DNA extracted with the QIAamp DNA FFPE Advanced Kits suitable for downstream applications that require more intact DNA such as long range PCR (>1 kb) and long read DNA sequencing? 153891 View What are recommended stopping points in the procedure of the QIAamp DNA FFPE Advanced Kits? 153892 View Can samples be lysed overnight with Proteinase K in the QIAamp DNA FFPE Advanced procedure? 153893 View Can the second Proteinase K lysis step in the QIAamp DNA FFPE Advanced procedure be carried out at lower temperatures than 65°C? 153894 View Can the 2nd Prot K step be omitted? 153896 View What size of DNA can be expected? 153897 View What is the difference of buffer FTB versus Buffer ATL? 153895 View Is it possible to change voltage set-up from 110V to 230V on the QIAcuity instruments? 3761 View Can I see error codes on the instrument touchscreen? 3763 View What is the scope of a regular maintenance of the QIAcuity? 3765 View What is the impact of not applying the latest VPF? Can I reanalyze previously obtained results after installing the latest VPF? 3769 View Can I see on the QIAcuity Software Suite report file if the VPF (Volume Precision Factor) has been used or not? 3770 View Is it necessary to reanalyze a plate with VPF (Volume Precision Factor) that was already processed using a QIAcuity instrument that was purchased in 2020? 3771 View Can I prepare a dPCR reaction directly in QIAcuity Nanoplate? 3774 View How to prepare DNA prior to dPCR? 3778 View Can I use a custom master mix instead of a QIAGEN master mix? 3777 View What are the storage conditions and expiry date of QIAcuity consumables? 3780 View Can I use the QIAcuity Nanoplate in more than one runs? 3781 View What is the VPF (Volume Precision Factor)? 3784 View When and how often do I need a new VPF (Volume Precision Factor)? 3785 View Is a standard curve needed in dPCR? 3786 View Which database versions are used for CRISPR-Q Custom PCR Assay and CRISPR-Q Sanger Primers design? 3788 View Is there anything to consider when processing cultured cells on coated cultivation dishes? Are additional purification steps required to eliminate or reduce coating reagents such as Poly-L-Lysine? 3791 View Is there something to consider when working with transduced cells treated with Polybrene? 3790 View For sequencing application, does the lysate require purification? 3792 View Are the CRISPR-Q PCR Assays and the CRISPR-Q Sanger Primers on GeneGlobe validated? 3793 View Are the PCR products generated with CRISPR-Q Custom PCR Assays only applicable to analysis of editing efficiency by Sanger sequencing? 3794 View Can the new CRISPR PCR Assays be used for dPCR on the QIAcuity? Do you have data or a protocol? 3795 View What is the minimum cell number needed for cell lysis? 3789 View For the Sanger analysis tool, the customer needs to upload two .abi1 files of forward and reverse sequences? Is there any additional info that needs to be provided? 3796 View What is the sequence of the QIAseq miRNA NGS 5’ Adapter? FAQ ID - 3672 View What are recommended stopping points in the procedure of the EZ1&2 DNA FFPE Kits? 3753 View Are there other options for paraffin removal? 3754 View Can samples be lysed overnight with Proteinase K in the EZ1&2 DNA FFPE procedure? 3755 View Can the second Proteinase K lysis step in the EZ1&2 DNA FFPE procedure be carried out at lower temperatures than 65°C? 3756 View What is the difference between Buffer FTB and Buffer ATL? 3757 View Can the 2nd Proteinase K step be omitted? 3758 View What size of DNA can be expected? 3759 View Is the DNA extracted with the EZ1&2 DNA FFPE Kits suitable for downstream applications that require more intact DNA such as long-range PCR (>1 kb) and long-read DNA sequencing? 3760 View Do you recommend 1-step or 2-step real-time RT-PCR for gene expression analysis? FAQ ID -1056 View 3323 - Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube? FAQ ID - 3323 View Does salt concentration in the food matter when using the DNeasy mericon Food Kit? FAQ ID - 3346 View Show more results Back to top Contact QIAGEN Global contacts Technical Service Customer Care

Which real-time PCR kits are recommended downstream of the QuantiTect Whole Transcriptome Kit?

FAQ ID -1592

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What primers are used in the reverse-transcription step of the QuantiTect Whole Transcriptome procedure?

FAQ ID -1584

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Can the AllPrep DNA/RNA/Protein Mini Kit be used with fibrous or lipid tissues?

FAQ ID -1579

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What is the maximum amount of RNA that can be used for amplification with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1591

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What starting amount of RNA purified using an miRNeasy Kit should be used with the miScript System?

FAQ ID -1597

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Can RNA purified from formalin-fixed, paraffin-embedded (FFPE) tissue be used with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1583

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Can samples in Allprotect Tissue Reagent undergo repeated freeze-thaws without degradation of DNA, RNA, and protein?

FAQ ID -1575

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Can the QuantiFast Probe PCR +ROX Vial Kit be used on the ABI PRISM 7900 and Applied Biosystems 7500 cyclers?

FAQ ID -1555

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Does Buffer APP in the AllPrep DNA/RNA/Protein Mini Kit contain acetone?

FAQ ID -1576

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Does cDNA amplified with the QuantiTect Whole Transcriptome Kit correspond to full-length RNA transcripts?

FAQ ID -1585

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Can tissue incubated in Allprotect Tissue Reagent be used for morphological studies?

FAQ ID -1571

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Can less than 10 ng RNA be used with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1590

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Can cDNA prepared by any method be used as starting material in the ligation reaction of the QuantiTect Whole Transcriptome Kit?

FAQ ID -1589

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Can Allprotect Tissue Reagent be used to stabilize RNA, DNA and proteins in cells, bacteria, yeast, and plants?

FAQ ID -1574

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Can degraded RNA be used with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1586

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How much cDNA is sufficient for real-time PCR detection of miRNA transcripts generated with the miScript System?

FAQ ID -1598

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How many samples can be run in parallel with the Rotor-Gene Q real-time PCR instrument?

FAQ ID -1519

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What main input voltage is required for the QIAxcel Analyzer?

FAQ ID -1821

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Is there a quality difference between CompactPrep Plasmid Mega and Giga and the CompactPrep Plasmid Midi and Maxi Kits?

FAQ ID -1882

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The Gel Cartridge is installed and all status indicators for the QIAxcel System are good but when clicking the "RUN" button, nothing happens. The green highlight goes down the Sequence?

FAQ ID -1850

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Why does the copy function not apply relative to migration time and normalized area values after loading the DNA size marker table and adding the alignment marker peaks on the QIAxcel System?

FAQ ID -1832

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Can customers use their own DNA size marker with the QIAxcel System and if yes, what concentration should be used?

FAQ ID -1841

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Can data be saved at once with the QIAxcel System after analysis of all samples in a 96-well PCR plate?

FAQ ID -1861

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How can a positive threshold be set when running the QIAxcel System?

FAQ ID -1843

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How long will the intensity calibration procedure on the QIAxcel System take?

FAQ ID -1826

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Will the QIAxcel System run using a green Data Review Software Key?

FAQ ID -1853

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When should an intensity calibration be re-performed on the QIAxcel System?

FAQ ID -1825

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Can the QIAxcel System or QIAxcel Advanced be connected to a network?

FAQ ID -1862

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How can a clogged capillary channel be identified when running samples on the QIAxcel System or QIAxcel Advanced?

FAQ ID -1836

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Can the QIAxcel software be loaded to another PC to view the data generated with the QIAxcel System?

FAQ ID -1855

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Can multiple QIAxcel Systems be run from one computer?

FAQ ID -1860

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How often should the solutions in the Buffer Tray for the QIAxcel System or QIAxcel Advanced be replaced?

FAQ ID -1840

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How often should the alignment marker for the QIAxcel System be changed?

FAQ ID -1839

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If the M500 method is used with the QIAxcel System, does a new DNA reference marker table have to be created?

FAQ ID -1856

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In the results table generated with the QIAxcel System, what does "normalized area" mean?

FAQ ID -1854

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What is the composition of buffer OCD?

FAQ ID -423

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What is the composition of buffer QN?

FAQ ID -414

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What is the composition of buffer OCL?

FAQ ID -422

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What is the composition of buffer OL2?

FAQ ID -424

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Can the QIAamp MinElute Virus Kits be used to extract viral nucleic acid from cell culture supernatant?

FAQ ID -472

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Can RNAi experiments be performed in Drosophila?

FAQ ID -436

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How is the RNeasy Lipid Tissue Mini Kit different from the RNeasy Mini Kit?

FAQ ID -468

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Has RNAi been successful using siRNA in Zebrafish and Xenopus?

FAQ ID -400

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Are Northern Blots sensitive enough to detect siRNA-induced gene silencing?

FAQ ID -403

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Can I use the RNeasy MinElute Cleanup Kit to clean up my in vitro transcription reaction?

FAQ ID -429

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What is the composition of buffer OEB?

FAQ ID -420

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Why is the pQE DNA provided in QIAexpress Kits blue in color?

FAQ ID -487

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What is the minimum elution volume when using the RNeasy Micro Kit?

FAQ ID -428

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What is the maximum amount of starting material that can be processed with the RNeasy Micro Kit?

FAQ ID -426

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What is the minimum elution volume when using the RNeasy MinElute Cleanup Kit?

FAQ ID -432

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What is the smallest sample size that can be used with RNeasy Mini Kits?

FAQ ID -485

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What is a homogeneous LiquiChip assay?

FAQ ID -476

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What detection reagents (reporter fluorophores) do you recommend for the LiquiChip System?

FAQ ID -480

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What are the excitation and emission wavelengths of the reporter and classification lasers of the LiquiChip Instrument?

FAQ ID -479

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What is the binding capacity of the RNeasy MinElute Cleanup Kit column?

FAQ ID -430

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What is the composition and size of the LiquiChip Beads?

FAQ ID -475

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What is the binding capacity of the RNeasy Micro Kit column?

FAQ ID -427

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Is dihydrofolate reductase (DHFR) immunogenic?

FAQ ID -471

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What are some of the assays that are adaptable to the LiquiChip System?

FAQ ID -477

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My QuantiTect Master Mix did not freeze at degrees. Is it still ok to use?

-20

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I want to start with gene silencing (RNAi) experiments. Do you have informational literature?

FAQ ID -580

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Is it possible to use less than 1 mg of protein with the PhosphoProtein Purification Kit?

FAQ ID -599

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Is it possible to use a QIAzol lysate for the isolation of RNA from tissue on the BioRobot EZ1?

FAQ ID -573

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What is a quenched FRET assay?

FAQ ID -542

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What do you recommend for isolating RNA from more than 100 mg of plant tissue?

FAQ ID -570

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What happens if I spin my lysate on the RNeasy Spin Columns at maximum speed?

FAQ ID -514

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How should I dissolve the lyophilized Anti-His and Tag Antibodies?

-100

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Is it possible to perform a UNG treatment when using QuantiTect kits?

FAQ ID -564

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Is it possible to clean up a methylation reaction containing bisulfite with QIAquick Cleanup Kits?

FAQ ID -519

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Is it possible to isolate both RNA and recombinant 6xHis-tagged protein from the same sample?

FAQ ID -532

View

Why are my realtime PCR amplification plots hook-shaped?

FAQ ID -587

View

Why are samples centrifuged at 4°C after the addition of chloroform when using RNeasy Lipid Tissue Kits?

FAQ ID -533

View

How was it determined that the PhosphoSerine Antibody will bind phosphoserines only?

FAQ ID -579

View

If pQE40 is used to express a fusion protein with DHFR, can the DHFR be cleaved afterwards?

FAQ ID -513

View

Which chloroform should I use for the RNeasy Lipid Tissue Kit?

FAQ ID -516

View

Will pancreas tissue swim on top of RNAprotect Tissue Reagent?

FAQ ID -536

View

Will the 2x QIAGEN Multiplex PCR Master Mix freeze at -20°C?

FAQ ID - 525

View

Why does my realtime PCR assay quality decrease over time?

FAQ ID -589

View

What is the longest fragment that was cloned with the pQE UA Cloning Vector?

-30

View

What is the difference between BSA-free and BSA-containing RGS-His Antibody?

FAQ ID -569

View

When using QIAzol Lysis Reagent for RNA extraction, I see a pinkish colored aqueous phase! Is it OK?

FAQ ID -534

View

Where can I find the public Smith-Waterman homology search tool that you refer to on your siRNA online design page?

FAQ ID -562

View

What reaction volume should I use with QuantiTect Kits?

FAQ ID -551

View

What makes QIAGEN's 10x Taq and HotStarTaq DNA Polymerase PCR buffer superior?

FAQ ID -566

View

How many template copies can I detect using QuantiTect Kits in real-time PCR?

FAQ ID -543

View

Can I use RNAiFect for co-transfection of plasmid DNA and siRNA?

FAQ ID -515

View

Does DEPC harm RNA?

FAQ ID -529

View

Does endogenously expressed DAPase in human cells degrade proteins that are expressed in cell culture?

FAQ ID -527

View

Can the MAPK1 control siRNA be used for rat cells?

FAQ ID -547

View

Are the columns of the MinElute Reaction Cleanup-, Gel Extraction-, and PCR Purification Kit identical?

FAQ ID -581

View

After using your U/A PCR Cloning Kit I can detect colonies, which are light blue, and not really white. Why is that?

FAQ ID -512

View

Can I make a master mix with QuantiProbes, primers, and all other reaction components and store it for later use?

FAQ ID -584

View

Can I homogenize samples in QIAzol using the QIAshredder?

FAQ ID -501

View

Can I shorten the activation time for the HotStarTaq DNA Polymerase?

FAQ ID -565

View

Does QIAGEN sell 6xHis-tagged proteins besides the 6xHis Protein Ladder?

FAQ ID -594

View

Do you offer QIAshredder spin columns for the RNeasy Midi/Maxi Kit format? If not, what is the alternative?

FAQ ID -560

View

Do you provide control proteins for use with the PhosphoProtein Purification Kit?

FAQ ID -596

View

How long should the amplicon be if I am using QuantiTect SYBR Green detection?

FAQ ID -553

View

How is an antibody epitope determined?

FAQ ID -593

View

Do I need to calibrate my real-time cycler if I want to use Yakima Yellow?

FAQ ID -540

View

Do we recommend pooling siRNA sequences?

FAQ ID -517

View

Does the His-tag interfere with the purification procedure of the QIAGEN PhosphoProtein Purification Kit?

FAQ ID -598

View

Does the use of MAPK1 as a control in siRNA transfection experiments have secondary effects on the cell culture?

FAQ ID -548

View

Do you have a protocol to isolate RNA from blood and bone marrow using the BioRobot EZ1?

FAQ ID -567

View

Do you have any recommendations for using the PosphoProtein Kit with starting material other than cells?

FAQ ID -597

View

Do you have a protocol for isolating RNA from yeast using RNeasy?

FAQ ID -535

View

What is the run time for the BioSprint 96 DNA Blood protocol on the BioSprint 96 instrument?

FAQ ID -622

View

Will REPLI-g work at high temperatures?

FAQ ID -656

View

The Multiflow Dispense Head on my BioRobot is not dispensing the correct amount of liquid. What can I do?

FAQ ID -652

View

What is the smallest amount of DNA I can put into a 50 ul REPLI-g reaction?

FAQ ID -663

View

What is the recommended incubation time to stabilize tissue RNA in RNAprotect Tissue Reagent?

FAQ ID -647

View

What do you mean by “Quantification of locus representation” in REPLI-g WGA product?

FAQ ID -698

View

What is REPLI-g whole genome amplification?

FAQ ID -654

View

What is the average amount of DNA and RNA present in 1 ml normal serum?

FAQ ID -635

View

What is the biggest protein that can be synthesized with the EasyXpress Protein Synthesis System?

FAQ ID -601

View

What is the length of the MDA whole genome amplification product?

FAQ ID -690

View

What is the expected yield of genomic DNA isolated from bacteria with the DNeasy Blood & Tissue or QIAamp DNA Mini Kit?

FAQ ID -632

View

Is it possible to perform laser microdissection with tissues stabilized with RNAprotect Tissue reagent?

FAQ ID -608

View

Is it possible to synthesize membrane proteins using the EasyXpress Protein Synthesis Kit?

FAQ ID -602

View

What are Elution Microtube adapters and Microplate adapters used in the DirectPrep 96 Miniprep Kit, and how can I order them?

FAQ ID -646

View

What are the advantages of REPLI-g over conventional DNA sample processing and amplification methods?

FAQ ID -686

View

Why do I get amplification in a negative control DNA tube using the REPLI-g Kit for WGA?

FAQ ID -675

View

Why did you choose MAPK1 as a positive control in the QIAGEN RNAi Human/Mouse Control Kit?

FAQ ID -604

View

Will the PhosphoSerine antibody recognize Serine residues if the neighboring residues are Leucine or Isoleucine?

FAQ ID -644

View

Will TA cloning work with REPLI-g WGA product?

FAQ ID -672

View

Will DNA from chromosomes collected in TE with a drop of mineral oil work with REPLI-g amplification?

FAQ ID -664

View

What is the stability of the REPLI-g MDA product?

FAQ ID -693

View

When delivering liquid to a 96-well plate using the MultiFlow Dispenser Head on my BioRobot , one or more rows in the plate contains less liquid per well than specified by the protocol. What is going wrong?

FAQ ID -653

View

Where should I add a dye-label modification on the siRNA?

FAQ ID -648

View

What types of materials from environmental samples could interfere with WGA?

FAQ ID -666

View

Can I stabilize RNA in bacteria grown on solid media or substrate using the RNAprotect Bacteria Reagent?

FAQ ID -615

View

Can I re-amplify whole genome amplification product?

FAQ ID -670

View

Can I use another lysis buffer than the one supplied in the PhophoProtein Purification Kit?

FAQ ID -645

View

Can I run less than 15 samples on the BioSprint 15 instrument?

FAQ ID -621

View

Can the QIAshredder Maxi Spin Columns from the DNeasy Plant Maxi Kit be used for RNeasy samples?

FAQ ID -616

View

Do you have an off-the shelf diagnostics kit for WGA?

FAQ ID -678

View

Are there components in biological samples that can inhibit the REPLI-g reaction?

FAQ ID -667

View

Are the BioSprint 15/96 instruments stand-alone workstations?

FAQ ID -620

View

Can the computer for the LiquiChip Workstation be networked?

FAQ ID -629

View

Are QIAGEN EZ Competent Cells of the QIAGEN PCR Cloning Kit dam+ or dam-?

FAQ ID -638

View

Are samples that are contaminated with high molecular weight DNA (e.g. from a paramedic or police officer) a problem with REPLI-g amplification?

FAQ ID -683

View

Can BAC DNA prepared with the R.E.A.L. Prep 96 Plasmid Kit be used for high-throughput sequencing?

FAQ ID -643

View

Can I do whole genome amplification from brain tissue using REPLI-g?

FAQ ID -669

View

Can I do whole genome amplification from mitochondrial DNA using REPLI-g?

FAQ ID -668

View

Can FTA paper for dried blood spots be used with REPLI-g?

FAQ ID -685

View

How can I quantify the amount of REPLI-g DNA I have amplified?

FAQ ID -694

View

Can I amplify from plasma or serum samples using the REPLI-g Mini/Midi kits?

FAQ ID -671

View

How should I store the REPLI-g amplification product?

FAQ ID -692

View

Is 2 kb the minimal gDNA fragment size for REPLI-g Whole Genome Amplification?

FAQ ID -682

View

How long can I store bacterial culture stabilized in RNAprotect Bacteria Reagent?

FAQ ID -614

View

Do I need to clean up the REPLI-g amplification product for downstream analysis?

FAQ ID -691

View

Do you have a kit for RNA isolation from any kind of sample type?

FAQ ID -627

View

Does REPLI-g technology for whole genome amplification work with paraffin embedded samples?

FAQ ID -673

View

How can I determine the quality of my REPLI-g amplified products?

FAQ ID -695

View

How can I decrease ribosomal RNA (rRNA) contamination using Oligotex?

FAQ ID -642

View

Do you know of anything in the buffers/DNA sample that can cause bias in REPLI-g WGA?

FAQ ID -684

View

Do you have a protocol for the purification of DNA from rodent tail using the BioSprint System?

FAQ ID -979

View

Do you have a protocol for the isolation of DNA from epithelial cells mixed with sperm cells?

FAQ ID -926

View

Do you have a protocol for isolation of genomic DNA from saliva using the DNeasy Blood & Tissue Kit?

FAQ ID -931

View

Do you have a protocol for purification of DNA from clotted blood using the FlexiGene DNA Kit?

FAQ ID -973

View

Do you have a protocol for Streptokinase treatment of clotted blood?

FAQ ID -912

View

Do you have a protocol for the isolation of total nucleic acids from animal and human cells on the BioRobot EZ1?

FAQ ID -974

View

Do you have a protocol for the isolation of total RNA from plant tissue using QIAGEN-tips?

FAQ ID -949

View

Do you have a protocol for the isolation of total RNA from buccal swabs?

FAQ ID -956

View

Do you have a protocol for the isolation of viral DNA from stool?

FAQ ID -929

View

Do you have a protocol for the isolation of total RNA from plants using the RNeasy 96 Kit?

FAQ ID -951

View

Do you have a protocol for the isolation of DNA from formalin-preserved stool samples?

FAQ ID -922

View

Do you have a protocol for the isolation of genomic DNA from fungi?

FAQ ID -911

View

Do you have a protocol for the isolation of genomic DNA and/or proteins from fatty tissue treated with QIAzol?

FAQ ID -955

View

Do you have a protocol for the purification of DNA from cells using the BioSprint System?

FAQ ID -977

View

Do you have a protocol for the purification of DNA from dried blood spots using the BioSprint System?

FAQ ID -982

View

Do you have a protocol for the preparation of tissues preserved in RNAprotect Tissue Reagent for pathological studies?

FAQ ID -950

View

Do you have a protocol for the purification of DNA from buccal swabs using the BioSprint System?

FAQ ID -983

View

Do you have a protocol for isolation of genomic and viral DNA from lymphocytes using QIAGEN Genomic-tips?

FAQ ID -901

View

Do you have a protocol for isolation of genomic and plasmid DNA from cell cultures using QIAGEN Genomic-tips?

FAQ ID -902

View

Do you have a protocol for transfection of adherent cells with low concentrations of siRNA using RNAiFect Transfection Reagent?

FAQ ID -970

View

Do you have a protocol for GMO testing of food samples?

FAQ ID -921

View

Do you have a protocol for isolation of bacterial DNA from soil?

FAQ ID -923

View

Do you have a protocol for isolation of genomic DNA from dried blood spots using the QIAamp 96 DNA Blood Kit?

FAQ ID -919

View

Do you have a protocol for isolation of genomic DNA from insects?

FAQ ID -904

View

Do you have a protocol for detection of HBV DNA by PCR?

FAQ ID -915

View

Does QIAGEN sell a vector with a T7 promotor for use with the EasyXpress Protein Synthesis Insect Kit?

FAQ ID -937

View

Can the BioSprint instruments be placed in a cool room?

FAQ ID -938

View

Do you have a protocol for Acetyl Cysteine (NALC) treatment of viscous samples?

FAQ ID -913

View

Do you have a protocol for co-transfection of adherent cells with siRNA and plasmid DNA?

FAQ ID -969

View

Do you have a protocol for adding new reporter dyes to the ABI PRISM 7900 Sequence Detection System?

FAQ ID -965

View

Do you have a protocol for cleanup of non-radioactive DNA samples?

FAQ ID -945

View

Do you have a protocol for radioactive labelling of cDNA?

FAQ ID -960

View

Do you have protocols for multiple extractions of DNA fragments from agarose gels?

FAQ ID -944

View

Why is it not recommended to stabilize cells with RNAprotect Tissue Reagent?

FAQ ID -941

View

Do you have a protocol for the detection of Bordetella pertussis DNA by PCR?

FAQ ID -914

View

Do you have a protocol for the isolation of DNA from cultured cells using the BioRobot EZ1?

FAQ ID -990

View

Do you have a protocol for performing quantitative multiplex PCR on the LightCycler 2.0 system?

FAQ ID -966

View

Do you have a protocol for long-term, siRNA-mediated gene silencing using HiPerFect Transfection Reagent?

FAQ ID -972

View

Do you have a protocol for manual purification of 6xHis-tagged proteins expressed in E. coli using Ni-NTA Superflow?

FAQ ID -967

View

Do you have a protocol for purification of DNA from cultured animal cells using the DNeasy 96 Blood & Tissue Kit?

FAQ ID -934

View

Do you have a protocol for purifying 2x96 PCR samples simultaneously with the QIAquick 96 PCR Purification Kit?

FAQ ID -935

View

Do you have a protocol for purification of DNA fragments from Cy3/Cy5 dye-label reactions?

FAQ ID -947

View

Do you have a protocol for polyacrylamide gel analysis of oligonucleotides?

FAQ ID -961

View

Do you have a protocol for isolation of genomic DNA from plants and filamentous fungi using QIAGEN Genomic-tips?

FAQ ID -905

View

Do you have a protocol for isolation of genomic DNA from flies using QIAGEN Genomic-tips?

FAQ ID -903

View

What are exo-resistant random hexamers used in the REPLI-g reaction?

FAQ ID -710

View

The Sigma Centrifuge makes a loud noise at low speeds which disappears at higher speeds, when using the 2x96-well plate rotor. What is the problem?

FAQ ID -776

View

Is RNAprotect Bacteria Reagent compatible with the RNeasy Mini Kit?

FAQ ID -797

View

What is the basic technology behind the Strep-tag Protein Purification System?

FAQ ID -740

View

What are the expected average DNA yields from plant extractions on the BioSprint 96?

FAQ ID -771

View

If template abundance differs significantly between housekeeping and target gene, are both amplified equally efficient with the QuantiTect Multiplex Kits?

FAQ ID -718

View

How to quantitate DNA purified using the BioSprint 96?

FAQ ID -773

View

How to store DNA from forensic samples eluted into water on the EZ1 biorobot in TE buffer instead?

FAQ ID -734

View

Is it possible to use LiquiChip beads for FACS applications?

FAQ ID -733

View

Is it possible to buy the EZ Competent Cells of the QIAGEN PCR Cloning Plus Kit separately?

FAQ ID -763

View

Is GFP excited and detected by the green laser of the LiquiChip System?

FAQ ID -737

View

Which Qproteome or Protein Fractionation Kits from QIAGEN are compatible with tissue samples?

FAQ ID -755

View

Why are some of my RNAprotect-stabilized samples frozen at 0ºC while others are not?

FAQ ID -758

View

Why are the QuantiTect and QuantiFast Multiplex PCR Kits limited to triplex real-time PCR on some cyclers?

FAQ ID -715

View

Why should DNA or cDNA targets be less than 250 bp long for real-time PCR?

FAQ ID-751

View

Where can I find info on compatible reporter dyes for use in real-time multiplex PCR using the QuantiTect Multiplex PCR Kits?

FAQ ID -719

View

Why might my gene of interest drop out during WGA if it is not near a telomere or centromere?

FAQ ID -703

View

Will the random hexamers in the REPLI-g reaction interfere with downstream analysis?

FAQ ID -713

View

Why is there no detection of free reporter fluorescence in the LiquiChip System?

FAQ ID -732

View

What is the cell number range one can use with the FastLane Cell cDNA Kit for real-time RT-PCR?

FAQ ID -782

View

What negative (unphosphorylated) and positive (phosphorylated) control proteins do you recommend for use with the PhosphoProtein Purification Kit?

FAQ ID -779

View

What is the enzyme used in the REPLI-g reaction?

FAQ ID -704

View

What type of Sequencing Services does QIAGEN offer?

FAQ ID -781

View

Where can I find a copy of the report files generated by running accessory protocols "Dilutor Delivery (monthly)" and "High-Speed Pipetting Sytem (monthly)" on my BioRobot 9604?

FAQ ID -766

View

What is the general sensitivity level of the LiquiChip System?

FAQ ID -731

View

Can QIAGEN's Taq- and HotstarTaq DNA Polymerases be used for cycle sequencing?

FAQ ID -741

View

Can I use the PhosphoProtein Purification Kit to purify phosphorylated peptides of around 1 kD size?

FAQ ID -778

View

Can RNAprotect Tissue Reagent be used to stabilize tissue which has already been flash-frozen?

FAQ ID -757

View

Can lyophilized plant material be used for DNA extraction on the BioSprint 96?

FAQ ID -772

View

Can the REPLI-g WGA enzyme work with labeled dNTPs?

FAQ ID -706

View

Does the QIAamp DSP Virus Kit require the QIAvac 24 Plus, or can I use it with my own laboratory vacuum system?

FAQ ID -789

View

Can the MAPK1 siRNA of the RNAi Human/Mouse Starter Kit be purchased separately?

FAQ ID -744

View

Can the QIAvac 96 vacuum manifold be used with DirectPrep 96 plates?

FAQ ID -722

View

Are the buffers in the Ni-NTA Fast Start Kit the same as the ones for use with Ni-NTA purchased separately?

FAQ ID -791

View

Any data on the fidelity of the REPLI-g MDA technique?

FAQ ID -707

View

Are Centromeres and Telomeres amplified using REPLI-g WGA?

FAQ ID -702

View

Can I use my own primers for REPLI-g WGA to amplify a specific chromosomal region?

FAQ ID -712

View

Can I do 4-plex real-time PCR, on the ABI PRISM 7000, 7700, or 7900 using the QuantiTect Multiplex PCR Kit?

FAQ ID -716

View

How can one perform multiplex real-time PCR analysis using the LightCycler 2.0?

FAQ ID -780

View

How can I be sure that I am harvesting my induced bacterial culture at the best time point for protein expression?

FAQ ID -788

View

How do the FastLane Cell cDNA Kit and QuantiTect Reverse Transcription Kit eliminate genomic DNA contamination?

FAQ ID -783

View

How many cells can be used with the FastLane Cell cDNA Kit?

FAQ ID -796

View

Do you have an antibody for Western Blot Analysis of MAPK1 gene knockdown using your MAPK1 control siRNA?

FAQ ID -746

View

Can frozen blood be used for whole genome amplification with REPLI-g?

FAQ ID -794

View

Do I have to switch off the laser of the LiquiChip Workstation overnight?

FAQ ID -736

View

How many reporter dyes can be used in the same analysis using the LiquiChip Workstation?

FAQ ID -738

View

Do I need to determine limiting primer concentrations with the QuantiTect Multiplex PCR Kits?

FAQ ID -714

View

Do you synthesize thiophosphate modified random hexamers?

FAQ ID -711

View

Has anyone verified whole genome amplification accuracy with Sequencing?

FAQ ID -701

View

Do you have a protocol for the fixation of cells transfected with fluorescently labeled siRNA?

FAQ ID -793

View

How best to handle the light sensitive LiquiChip reagents?

FAQ ID -735

View

Do you have information on the use of recombinant DNA and RNA as absolute standards for realtime RT-PCR?

FAQ ID -729

View

Is it possible to transfect DNA into insect cell lines such as Drosophila melanogaster S2?

FAQ ID -397

View

Is it possible to isolate free genomic DNA using the QIAamp UltraSens Virus Kit?

FAQ ID -358

View

Is it possible to store TAGzyme enzymes at°C?

-80

View

I accidentally added RNAprotect Tissue Reagent to my cells, and now the cells are difficult to pellet. What can I do?

FAQ ID -364

View

What are the calculated molecular weights of the TAGZyme enzymes?

FAQ ID -329

View

How complete is the removal of DAPase in the TAGZyme process?

FAQ ID -319

View

How long is fluorescence detectable in cells after transfection with fluorescently labeled siRNAs?

FAQ ID -392

View

How do I submit a siRNA order by telephone or online?

FAQ ID -399

View

How many transfections can I perform with 20 nmol of HPP grade siRNA?

FAQ ID -367

View

Which primers can I use for sequencing pQE-expression vector constructs?

FAQ ID -343

View

What is the standard assay volume recommended for LiquiChip assays?

FAQ ID -330

View

What is the acceptable maximum g force for centrifugation of Xa Removal Resin?

FAQ ID -320

View

What is the lowest elution volume that can be used with QIAprecipitator Midi and Maxi Modules?

FAQ ID -307

View

What is the composition of Buffer FG3?

FAQ ID -356

View

Can I use ethanol instead of isopropanol for DNA precipitation when using HiSpeed Plasmid Kits?

FAQ ID -354

View

303 - Can I use my own lysis buffer with the DNeasy Blood & Tissue or QIAamp DNA Mini Kit?

View

Can I use a partial MinElute 96 UF plate?

FAQ ID -355

View

Can I use the RNeasy Mini Kit or RNeasy 96 Kit with fewer than 100 cells?

FAQ ID -372

View

Can I use water for elution from the QIAprecipitator of the HiSpeed Plasmid Kits?

FAQ ID -306

View

Are the QIAprecipitator Midi and Maxi Modules of the HiSpeed Plasmid Kits interchangeable?

FAQ ID -308

View

Apart from the antibody itself, does the Penta-His Biotin Conjugate solution contain any protein?

FAQ ID -359

View

Are QIAGEN monoclonal Anti His Antibodies glycosylated?

FAQ ID -334

View

Can I perform in-vitro transcription with the pDrive Cloning Vector from the QIAGEN PCR Cloning Kit?

FAQ ID -332

View

Do the QuantiTect SYBR Green Kits contain a stabilization reagent that inhibits the sensitivity of real-time PCR?

FAQ ID -328

View

Do I need to anneal, deprotect or desalt my QIAGEN siRNA?

FAQ ID -398

View

How are siRNAs introduced into C. elegans?

FAQ ID -396

View

Do pQE vectors contain the CAT gene?

FAQ ID -362

View

How can I improve the expression of proteins containing hydrophobic regions?

FAQ ID -339

View

How can I check if any residual proteins remain on the Ni-NTA Agarose matrix after elution?

FAQ ID -324

View

Do freeze-thaw cycles affect the stability of the U-overhang on the pDrive Cloning Vector?

FAQ ID -323

View

Can RNAprotect Tissue Reagent be used to store previously isolated RNA?

FAQ ID -337

View

Can MinElute 96 UF PCR be used to purify PCR samples which contain mineral oil?

FAQ ID -360

View

Does SYBR Green dye present in QuantiTect kits interfere with ethidium bromide staining?

FAQ ID -312

View

Do any of the buffers in the HotStarTaq DNA Polymerase Kit contain Triton?

FAQ ID -327

View

Do any of the kit components or the product packaging of the QIAamp 96 DNA Blood Kit contain latex?

FAQ ID -318

View

What data documentation does QIAGEN's Sequencing Service provide for customers?

FAQ ID -832

View

What are the sequences of the FlexiTube siRNAs?

FAQ ID -851

View

My BioRobot 9604 came with several Accessory Applications. Is it recommended I run these protocols?

FAQ ID -872

View

What is the proper way to turn on or turn off the EZ1 instrument?

FAQ ID -870

View

What is the Energy Mix in the EasyXpress Kits?

FAQ ID -846

View

What is the concentration of the primers in a reaction using the QuantiTect Primer Assays?

FAQ ID -850

View

I would like to sequence my siRNA constructs. Does QIAGEN offer Sequencing Services for cloned siRNA?

FAQ ID -829

View

Is it possible to use frozen cell pellets with the FastLane Cell cDNA Kit?

FAQ ID -835

View

Is it possible to elute plasmid DNA from the QIAprep Spin Miniprep columns with buffer containing Potassium Phosphate?

FAQ ID -854

View

Is it possible to perform immunoprecipitation (IP) directly on protein fractions from the Qproteome Cell Compartment Kit?

FAQ ID -855

View

Is it possible to adjust the Lysis Buffer volume used with the Qproteome Bacterial Protein Preparation Kit?

FAQ ID -841

View

Is it possible to use QIAGEN's pQE-TriSystem Vectors with the EasyXpress Protein Synthesis Insect Kit?

FAQ ID -876

View

Is there a stopping point in the protocol for Suspended Cells using the FastLane Cell cDNA Kit?

FAQ ID -837

View

Is the random biotin labeling of proteins expressed with your EasyXpress Biotin Labeling Kit as sensitive as labeling with radioactivity?

FAQ ID -875

View

Will biotin detection assays be efficient when doing in vitro translation from E. coli lysates with the EasyXpress Site-Specific Biotin Kit?

FAQ ID -878

View

Why would clumps occur following the addition of Buffer P2 when using LyseBlue Reagent in a plasmid preparation?

FAQ ID -862

View

Will acetone precipitation recommended in the Qproteome Protocols denature Protein?

FAQ ID -807

View

Will site-specific biotin labeling with the EasyXpress Site-Specific Biotin kit alter the structure and function of my protein?

FAQ ID -877

View

Will the sequences of the QuantiTect Primer Assays be provided?

FAQ ID -804

View

Will the FastLane Cell cDNA Kit work with suspension cells?

FAQ ID -801

View

Which resin is used in the QIAexpress Ni-NTA Fast Start Columns?

FAQ ID -836

View

What publicly funded Genome Sequencing Projects has QIAGEN's Sequencing Service been involved in?

FAQ ID -815

View

What is the typical yield of site-specific labeled protein generated using the EasyXpress Site-Specific Biotin Kit?

FAQ ID -873

View

What programs does your Sequencing Service use to ensure accurate base calling, Sequence Quality, and Alignment?

FAQ ID -831

View

Where can I find a copy of the report file generated by running 'Maintenance Protocol I' on my BioRobot MDx?

FAQ ID -806

View

Which fraction will contain soluble mitochondrial proteins using the Qproteome Cell Compartment Kit?

FAQ ID -839

View

Which fraction will contain endosomal, microsomal and lysosomal proteins using the Qproteome Cell Compartment Kit?

FAQ ID -808

View

Where can I find QIAGEN products for a specific gene or gene product?

FAQ ID -803

View

Can the QuantiTect Primer Assays be used for detection of genomic DNA?

FAQ ID -811

View

Do I need to use RNase inhibitors with the RNeasy Kits?

FAQ ID -813

View

Do the Buffers CE1 - CE4 of the Qproteome Cell Compartment Kit contain DTT or any reducing agent?

FAQ ID -824

View

Can the FastLane Cell cDNA Kit also be used for qualitative end-point PCR?

FAQ ID -834

View

Can the FastLane Cell cDNA Kit be used with tissue?

FAQ ID -821

View

Do you have a protocol for the isolation of bacteriophage P1 derived constructs with any of your plasmid kits?

FAQ ID -883

View

Do you have a protocol for the isolation of BAC DNA using the QIAGEN Plasmid Midi Kit?

FAQ ID -881

View

895 - Do you have a protocol for the isolation of endotoxin-free plasmid DNA using the QIAGEN Plasmid Midi Kit?

View

Do you have a protocol for isolation of plasmid DNA using the Sigma Centrifuge 4?

-15

View

Can Buffer R1 of the R.E.A.L. Prep 96 Plasmid Kit be replaced with Buffer P1?

FAQ ID -823

View

Are there specific recommendations for performing RT-PCR on RNA isolated from paraffin-embedded samples?

FAQ ID -828

View

Can I stabilize adipose tissue using RNAprotect Tissue Reagent prior to RNA isolation?

FAQ ID -856

View

Can I use the PhosphoProtein Purification Kit for the isolation of phosphorylated proteins from yeast?

FAQ ID -838

View

Can I use QIAGEN's pQE vectors as templates in the EasyXpress Biotin Labeling Kits?

FAQ ID -874

View

Can I use my gene-specific primers with the FastLane Cell cDNA Kit and the QuantiTect Reverse Transcription Kit?

FAQ ID -812

View

Can I use LyseBlue with R.E.A.L. Prep 96, QIAwell Ultra, or QIAprep 96 Turbo Miniprep Kits for the BioRobot systems?

FAQ ID -863

View

Do you have information on in vivo RNA interference experiments?

FAQ ID -817

View

Do you sell the ATP-Dependent Exonuclease of the QIAGEN Large-Construct Kit separately?

FAQ ID -825

View

I cannot see the LCD display fonts on the Sigma Centrifuge. How do I adjust the contrast?

FAQ ID -822

View

How long does a typical in vitro translation reaction take with your large-scale EasyXpress protein synthesis kits?

FAQ ID -880

View

How much protein can be produced with the EasyXpress Protein Synthesis Mega Kit?

FAQ ID -843

View

Do you have a protocol for the isolation of very low-copy plasmids from Streptomyces spp.?

FAQ ID -893

View

Do you have a protocol for the isolation of plasmid DNA from Citrobacter freundii?

FAQ ID -885

View

Do you have a protocol for the isolation of plasmid DNA from Corynebacterium glutamicum?

FAQ ID -886

View

Do you have a protocol for the isolation of plasmid DNA from Borrelia spp.?

FAQ ID -884

View

Do you have a protocol for the isolation of plasmid DNA from Lactobacillus spp.?

FAQ ID -887

View

Do you have a protocol for the isolation of plasmid DNA from yeast?

FAQ ID -853

View

Do you have a protocol for the isolation of single-stranded DNA from M13 phage using QIAGEN Plasmid Kits?

FAQ ID -894

View

Do you have a protocol for the isolation of plasmid DNA from Staphylococcus spp.?

FAQ ID -890

View

Do you have a protocol for the isolation of plasmid DNA from Oligotropha carboxidovorans?

FAQ ID -888

View

Do you have a protocol for the isolation of plasmid DNA from Proteus spp.?

FAQ ID -889

View

What are your recommendations for cotransfecting several plasmids?

FAQ ID -124

View

What are the recommended culture and buffer volumes for a very low-copy plasmid?

FAQ ID -168

View

Is mRNA isolation necessary for sensitive RT-PCR?

FAQ ID -111

View

When should carrier be used with the QIAamp DNA Mini or the DNeasy Blood & Tissue Kit?

FAQ ID -100

View

Which Anti-His Antibody is the most sensitive for my protein of interest?

FAQ ID -172

View

Why do I have to add beta-mercaptoethanol (beta-ME) to lysis Buffer RLT of the RNeasy Kits?

FAQ ID -101

View

What is the recommended culture medium for the QIAprep System?

FAQ ID -154

View

How can I eliminate contaminating protein in my Ni-NTA 6xHis-tag protein purification?

FAQ ID -102

View

How can I purify very small amounts of 6xHis-tagged protein using Ni-NTA technology?

FAQ ID -134

View

How can I improve ligation efficiency of DNA from a QIAEX II Gel Extraction Kit?

FAQ ID -141

View

How can I isolate RNA from 1 gram of plant tissue?

FAQ ID -128

View

How can I improve the performance of the HiSpeed QIAprecipitator module?

FAQ ID -144

View

What can I use to isolate RNA smaller than 200 nucleotides?

FAQ ID -115

View

What epitopes do the Anti-His Conjugates and Anti-His Antibodies recognize?

FAQ ID -170

View

What are the maximum culture volumes to use with the QIAGEN Plasmid Midi or Maxi Kit?

FAQ ID -167

View

How should RNeasy Kits be stored and how long are they stable?

FAQ ID -103

View

Is it possible to cleave the 6xHis-tag from an expressed protein?

FAQ ID -140

View

How should QIAGEN Plasmid Purification Kits be stored and for how long?

FAQ ID -192

View

What are the features and benefits of the QIAexpress 6xHis Tag System?

FAQ ID -193

View

Do you have transfection data for QIAGEN Transfection Reagents?

FAQ ID -158

View

Do you have data showing effects of sample size on DNA yield and purity using the DNeasy 96 Blood & Tissue kit?

FAQ ID -129

View

Does QIAGEN offer vectors for direct cloning of PCR products?

FAQ ID -146

View

How can I avoid variable transfection efficiencies with SuperFect Reagent?

FAQ ID -179

View

Can DyeEx be used to clean up labeled DNA other than sequencing templates?

FAQ ID -137

View

Are QIAamp DNA isolation kits suitable for apoptosis studies?

FAQ ID -149

View

Can I use a QIAfilter Cartridge for purifying large plasmids?

FAQ ID -121

View

Can I use Omniscript or Sensiscript RT's at a higher temperature?

FAQ ID -116

View

How do you ensure that RNeasy buffers are RNase-free?

FAQ ID -113

View

How can I purify DNA from soil, food and sewage samples for PCR?

FAQ ID -118

View

How should I store Anti·His and Tag·100 Antibodies?

FAQ ID -194

View

How do I prepare an insert for pQE vectors?

FAQ ID -185

View

How can I obtain DNA-free RNA using an RNeasy Midi or Maxi Kit?

FAQ ID -143

View

What are the compatibilities of different reagents with Ni-NTA matrices?

FAQ ID -49

View

How can I prevent running out of M48 Kit reagents before the full number of samples are processed?

FAQ ID -1042

View

Do you have a protocol for the isolation of genomic DNA from paraffin-embedded samples on the BioRobot M48?

FAQ ID -1019

View

Is the TurboCapture Microplate Holder Type B compatible with the Biomek FX robotic workstation?

FAQ ID -1040

View

Is there a limit to the size of proteins that can be purified using the Qproteome Glycoprotein Kits?

FAQ ID -1071

View

Is it possible to scale up QuantiTect Reverse Transcription reactions to allow use of larger amounts of RNA?

FAQ ID -1063

View

Is it possible to use the PhosphoProtein Purification Kit in batch format?

FAQ ID -1069

View

What are the recommended storage conditions for the QuantiTect Reverse Transcription Kit and its components?

FAQ ID -1077

View

What is the difference between TurboCapture Microplate Holders Type A and Type B?

FAQ ID -1038

View

What blocking reagents do you recommend for chemiluminescent detection using Penta-, Tetra-, and RGS-His antibodies?

FAQ ID -1093

View

Is Factor SB of the HotStar HiFidelity Polymerase Kit the same as Factor MP of the QIAGEN Multiplex PCR Kit?

FAQ ID -1049

View

How can I transfect siRNA into insect cells such as Drosophila melanogaster S2?

FAQ ID -1046

View

How can I tell TurboCapture Microplate Holder Type A from Type B?

FAQ ID -1039

View

How much template can I use in the reaction and what is the maximum volume of template that can be used in the QuantiTect Kits?

FAQ ID -1086

View

How much dye-labeled siRNA should I use to monitor transfection efficiency when using HiPerfect Transfection Reagent?

FAQ ID -1058

View

What is the error rate of HotStar HiFidelity DNA Polymerase?

FAQ ID -1048

View

Which QIAGEN kit do you recommend for purifying plasmid DNA suitable for transfection of sensitive cells?

FAQ ID -1092

View

Which kit should I use for RNA isolation from Cartilage?

FAQ ID -1026

View

Where can I find the serial number on QIAGEN's BioRobots?

FAQ ID -1064

View

Which kit can be used for simultaneous purification of DNA and RNA from biopsies?

FAQ ID -1089

View

Why is there RNA in the DNA eluate when using the AllPrep DNA Mini Spin Column?

FAQ ID -1044

View

Why does my PCR product show up later when comparing the QuantiTect SYBR Green PCR Kits with Roche kits using the same annealing temperature?

FAQ ID -1083

View

Will the "classic" RNeasy Mini Kit be discontinued after the launch of the Allprep DNA/RNA Mini Kit?

FAQ ID -1054

View

Why does TA/UA cloning work with your proofreading HotStar HiFidelity DNA Polymerase?

FAQ ID -1053

View

Why do melting temperatures differ between PCR fragments amplified with QIAGEN's QuantiTect SYBR Green PCR Kits and Roche Kits?

FAQ ID -1084

View

What is the range of melting temperatures for the QuantiTect Primer Assay amplicons in the dissociation curve?

FAQ ID -1079

View

What is the protein loading capacity per column of the Qproteome Albumin/IgG Depletion Kit?

FAQ ID -1090

View

What is your recommendation for cleaning the TissueLyser Bead Dispenser (96-well format)?

FAQ ID -1055

View

What vacuum pressure should be applied using the QIAvac 24 Plus for the QIAamp DSP Virus Kit and Blood Mini Kit Protocols?

FAQ ID -1033

View

What is the resin in the Qproteome Murine Albumin and Qproteome Albumin/IgG Depletion Kits?

FAQ ID -1034

View

How can I prevent clogging of QIAfilter cartridges?

FAQ ID -1060

View

What is the volume of the eluate when using either spin or vacuum procedures with the QIAprep 96 Turbo Miniprep Kit?

FAQ ID -1067

View

Do I have to use a ramp time of 2°C/sec on the LightCycler when using Quantitect Primer Assays?

FAQ ID -1065

View

Does an activation time of 15 minutes influence the performance of the HotStarTaq Plus DNA Polymerase?

FAQ ID -1050

View

Do you have a protocol for the isolation of RNA from bacterial cultures using RNAprotect Bacteria Reagent and QIAzol Lysis Reagent?

FAQ ID -1020

View

Do you have a protocol for the isolation of DNA from mouse tails using MagAttract technology on BioRobot M96?

FAQ ID -1007

View

Do you have a protocol for the isolation of DNA from forensic samples using the BioRobot M48?

FAQ ID -1017

View

Can the USB Port of the BioSprint 96 instrument be used to transfer programs from a PC to the instrument?

FAQ ID -1032

View

Are your FlexiTube siRNAs preferentially designed to any particular region of the target gene?

FAQ ID -1091

View

Can PCR fragments generated with the EasyXpress Linear Template Kit be cloned into the pIX4.0 vector of the EasyXpress Protein Synthesis Insect Kit?

FAQ ID -1078

View

Can the QProteome O-Glycan Glycoprotein Kits be used for blood cells?

FAQ ID -1036

View

Can the Reverse Transcriptases of the QuantiTect Reverse Transcription Kit and the QuantiTect Probe RT-PCR Kit be used interchangeably?

FAQ ID -1066

View

Can the AllPrep RNA/Protein Kit be used with tissue?

FAQ ID -1095

View

Can the Blocking Reagent supplied with the Penta-His HRP Conjugate be used with other antibodies?

FAQ ID -1073

View

How can air bubbles in PhosphoProtein Purification Columns be avoided?

FAQ ID -1070

View

How can I increase low-copy plasmid DNA yields using QIAfilter Plasmid Kits?

FAQ ID -1062

View

How can I ensure complete genomic DNA removal when using the RNase-Free DNase Set?

FAQ ID -1087

View

Do you have information on the reproducibility of DNA yields from blood using the M48 BioRobot?

FAQ ID - 1004

View

Do you have a protocol for transient transfection of HEK 293 cells in 75 cm3 flasks?

FAQ ID -1014

View

Do you have a protocol for transient transfection of cells in 96-well plates using PolyFect Transfection Reagent?

FAQ ID -1013

View

Do you have a protocol for transient transfection of HeLa-S3 cells in 60 mm dishes using PolyFect?

FAQ ID -1012

View

Do you have a protocol for the isolation of total nucleic acids from cells on the M48 BioRobot?

FAQ ID -1009

View

Do you have a protocol for vacuum processing of blood samples with the QIAamp DNA Blood Midi or Maxi Kits?

FAQ ID -1011

View

Do you have a protocol for the isolation of total nucleic acids from tissue on the M48 BioRobot?

FAQ ID -1008

View

Do you have information on realtime RT-PCR performance of RNA isolated from monocytes on the M48 BioRobot?

FAQ ID -1010

View

Do you have information on DNA yields from blood with variable numbers of white blood cells using the M48 BioRobot?

FAQ ID -1006

View

Do you have information on PCR template preparation from preserved blood samples on the M48 BioRobot?

FAQ ID - 1003

View

Can the PAXgene Blood RNA Tubes undergo freeze/thaw cycles?

FAQ ID -2471

View

If I want to use BioMag particles, do I have to buy a QIAGEN magnet too or can I use one that I already have?

FAQ ID -266

View

Should I use Omniscript or Sensiscript for reverse transcription of low-copy mRNA?

FAQ ID -297

View

What are the main differences between other magnetic beads and the BioMag particles?

FAQ ID -272

View

My BioMag is about to expire, is it still any good?

FAQ ID -268

View

How magnetically responsive is BioMag?

FAQ ID -265

View

If I am using BioMag Streptavidin to capture biotinylated oligos, how can I then remove the oligos?

FAQ ID -279

View

Why is an ice-incubation step included during reaction set-up when following the QuantiTect RT-PCR but not the QuantiTect PCR protocol.

FAQ ID -283

View

What sequencing primers can I use with the pDrive cloning vector of the QIAGEN PCR Cloning Kit?

FAQ ID -292

View

What is the difference between the QIAamp MinElute Virus Spin and the MinElute Virus Vacuum Kit?

FAQ ID -295

View

What is the difference between the Fc specific versions of your secondary antibody BioMag particles and the non-specific?

FAQ ID -275

View

Can I use my own cycling conditions with the QIAGEN Multiplex PCR Kit?

FAQ ID -287

View

Can I select FITC labeled cells with BioMag?

FAQ ID -278

View

Can I use BioMag particles with any specific primary antibody?

FAQ ID -273

View

Are BioMag particles stable in other solvents?

FAQ ID -267

View

Can BioMag be used for positive and negative selection?

FAQ ID -269

View

How can I avoid poor immunolocalization morphology with Anti-His Antibodies?

FAQ ID -200

View

How can I prevent non-specific binding when using BioMag?

FAQ ID -277

View

How do I prevent bubbles from forming in my Ni-NTA agarose column?

FAQ ID -285

View

How can I purify mRNA from total RNA using an Oligotex Direct mRNA Kit?

FAQ ID -201

View

Is your Nuclease-Free Water fluorescence-free?

FAQ ID -1291

View

What quality level does your Nuclease-Free Water have?

FAQ ID -1292

View

How should the GelPilot DNA Loading Dye be handled?

FAQ ID -1287

View

Does GelPilot DNA Loading Dye contain ethidium bromide?

FAQ ID -1286

View

Can phenol/chloroform be placed in the MaXtract tube for extraction at a later time?

FAQ ID -1299

View

Are MaXtract tubes siliconized?

FAQ ID -1298

View

Should I input the dilution factor of my samples into the LiquiChip Analyser Software dilution factors of samples?

FAQ ID -1211

View

What cell lines have been tested with the Qproteome Plasma Membrane Protein Kit?

FAQ ID -1205

View

Is a saliva collection device provided in the RNeasy Protect Saliva Mini Kit?

FAQ ID -1213

View

Is it possible to increase nucleic acid concentrations when following protocols on the BioRobot EZ1?

FAQ ID -1232

View

Is RNAprotect Cell Reagent provided in the RNeasy Protect Cell Kit available separately?

FAQ ID -1217

View

Is it possible to use QIAGEN's pQE-Trisystem Vector with the EasyXpress Insect Kit II?

FAQ ID -1225

View

What vector do you recommend as template for in-vitro protein expression with the EasyXpress Insect Kit II?

FAQ ID -1224

View

What type of promoter should my template have for in-vitro protein expression with the EasyXpress Insect Kit II?

FAQ ID -1223

View

What is the maximum number of cells that can be used with the AllPrep RNA/Protein Kit ?

FAQ ID -1208

View

Will the Qproteome Plasma Membrane Protein Kit work with starting materials other than adherent mammalian cells?

FAQ ID -1202

View

What is the difference between various FlexiTube siRNAs listed for the same target gene, and which one should I choose?

FAQ ID -1206

View

What is the protein yield when using the Qproteome Plasma Membrane Protein Kit?

FAQ ID -1201

View

What is the size of the BioRobot EZ1?

FAQ ID -1245

View

Does anything have to be added to express post-translational modifications (PTMs) with the EasyXpress Insect Kit II?

FAQ ID -1226

View

Do you have a protocol for isolating cytoplasmic RNA from animal cells using the RNeasy Mini Kit?

FAQ ID -1207

View

Do you have a protocol for purification of miRNA from animal cells using the RNeasy Plus Mini Kit and RNeasy MinElute Cleanup Kit?

FAQ ID -1258

View

Can cells stabilized with RNAprotect Cell Reagent be used for cell sorting by flow cytometry (FACS)?

FAQ ID -1216

View

Can glycosylated proteins be expressed using the EasyXpress Insect Kit II?

FAQ ID -1227

View

Can RNAprotect Saliva Reagent be purchased separately?

FAQ ID -1214

View

Can you recommend a Reagent or Kit for stabilizing RNA in cultured cells?

FAQ ID -1215

View

Can the Strep-Tactin Magnetic Beads of the Qproteome Plasma Membrane Protein Kit be reused?

FAQ ID -1204

View

Can the Qproteome Plasma Membrane Protein Kit be used with tissue samples?

FAQ ID -1200

View

Can the RLT lysate of the BioSprint DNA Plant protocol be stored frozen?

FAQ ID -1248

View

How can I decontaminate the BioRobot EZ1?

FAQ ID -1231

View

How do you ensure that concentrated RNA is purified from saliva using the RNeasy Protect Saliva Mini Kit?

FAQ ID -1212

View

How much active protein can be expressed in-vitro using the EasyXpress Insect Kit II, and how much time does it take?

FAQ ID -1222

View

How many freeze-thaw cycles can the various reaction mixes in the EasyXpress Insect Kit II tolerate?

FAQ ID -1219

View

Do you have a protocol for the isolation of DNA from buccal cells using the BioRobot EZ1?

FAQ ID -1260

View

Do you have a protocol for transfection of differentiated macrophage cell lines (THP) with siRNA?

-1

View

How are plasma membrane containing vesicles eluted off the ligand in the Qproteome Plasma Membrane Protein procedure?

FAQ ID -1203

View

Which fixation method is used in the PAXgene Tissue System?

FAQ ID - 3600

View

What is the fixation reagent of PAXgene Tissue FIX?

FAQ ID - 3601

View

What is the stabilization reagent of PAXgene Tissue STABILIZER?

FAQ ID - 3602

View

What is the maximum tissue size that can be fixed in a PAXgene Tissue FIX Container (50 ml)?

FAQ ID - 3604

View

Why are two reagents used in the PAXgene Tissue System?

FAQ ID - 3603

View

Which conditions are recommended for the storage of tissues in PAXgene Tissue STABILIZER?

FAQ ID - 3605

View

Is it possible to process formalin-fixed and PAXgene Tissue fixed samples together in one run?

FAQ ID - 3606

View

Is it necessary to clean a processor normally used for formalin-fixed tissue before using it with PAXgene Tissue fixed tissue?

FAQ ID - 3607

View

Is it possible to integrate the PAXgene Tissue STABILIZER into tissue processing?

FAQ ID - 3608

View

Are immunohistochemistry (IHC) assays developed for formalin-fixed, paraffin-embedded (FFPE) tissues compatible with PAXgene Tissue fixed, paraffin-embedded (PFPE) tissues?

FAQ ID - 3609

View

How well is RNA integrity preserved in PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?

FAQ ID - 3611

View

Is it possible to use formalin-fixed, paraffin-embedded (FFPE) kits and protocols to isolate biomolecules from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?

FAQ ID - 3610

View

How well is DNA integrity preserved in PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?

FAQ ID - 3612

View

Are special kits and protocols required to isolate biomolecules from PAXgene Tissue fixed, cryo-embedded (PFCE) tissue?

FAQ ID - 3614

View

What are the yield and integrity of nucleic acids isolated from blocks of PAXgene Tissue fixed, cryo-embedded (PFCE) tissue?

FAQ ID - 3613

View

Can proteins be extracted from PAXgene Tissue fixed specimens?

FAQ ID - 3616

View

Does the PAXgene Tissue RNA Kit co-purify small RNAs?

FAQ ID - 3615

View

Which kits and protocols can be used to isolate nucleic acids from microdissected PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) specimens?

FAQ ID - 3617

View

What is the PCR performance of DNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissue compared to DNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?

FAQ ID - 3618

View

How is the quality of proteins purified from PAXgene Tissue fixed and stabilized tissue?

FAQ ID - 3619

View

Can the RNeasy kits be used to extract RNA from saliva collected using the Oragene collection kit?

FAQ ID - 3623

View

How do I analyze codon 61 mutations with the RAS extension kit (cat. no. 971590)?

FAQ ID - 3622

View

Are QR codes recognized by QIAsymphony?

FAQ ID - 3625

View

Do I need to use a blood collection set with the PAXgene Blood ccfDNA Tube?

FAQ ID - 3626

View

What is the reagent in the PAXgene Blood ccfDNA Tube?

FAQ ID - 3632

View

Does the insert sequence provided in the specification sheet correspond to the target sequence or to anti-sense sequence?

FAQ ID - 3627

View

What is the shelf life of unused PAXgene Blood ccfDNA Tubes?

FAQ ID - 3633

View

How should unused PAXgene Blood ccfDNA Tubes be stored?

FAQ ID - 3634

View

Are there special considerations for phlebotomy when using the PAXgene Blood ccfDNA Tube (blood collection set, discard tube, positioning of the tube, issues with short draws, etc.)?

FAQ ID - 3635

View

Are PAXgene Blood ccfDNA Tubes sterile?

FAQ ID - 3636

View

What is the expected ccfDNA yield from plasma generated from 10 ml of blood?

FAQ ID - 3637

View

Can I use plasma from underfilled PAXgene Blood ccfDNA Tubes?

FAQ ID - 3638

View

How long can whole blood stabilized in PAXgene Blood ccfDNA Tubes be stored at room temperature?

FAQ ID - 3639

View

Can whole blood in PAXgene Blood ccfDNA Tubes be stored at temperatures above 25°C?

FAQ ID - 3640

View

Can whole blood in PAXgene Blood ccfDNA Tubes be stored at temperatures below 15°C?

FAQ ID - 3641

View

Can whole blood be frozen in PAXgene Blood ccfDNA Tubes?

FAQ ID - 3642

View

Is double centrifugation required for plasma processing?

FAQ ID - 3643

View

How long and at which temperature can plasma generated from whole blood in PAXgene Blood ccfDNA Tubes be stored?

FAQ ID - 3644

View

What should I do if cryoprecipitates form while thawing a frozen plasma sample?

FAQ ID - 3646

View

Which plasma volumes can I process with the QIAsymphony PAXgene Blood ccfDNA protocols?

FAQ ID - 3648

View

How many extractions can I perform with a QIAsymphony PAXgene Blood ccfDNA Kit, using the 2.4 ml or the 4.8 ml protocols?

FAQ ID - 3649

View

How long after first use can a reagent cartridge be stored for further use?

FAQ ID - 3650

View

For what type of sample material is the QIAsymphony PAXgene Blood ccfDNA Kit intended?

FAQ ID - 3652

View

Which tubes are recommended for the QIAsymphony sample rack

FAQ ID - 3653

View

Which protocol line should I use to process plasma samples?

FAQ ID - 3654

View

Does the QIAsymphony PAXgene Blood ccfDNA Kit also allow eluting ccfDNA in less than 60 µl?

FAQ ID - 3656

View

How should eluates produced with the PAXgene Blood ccfDNA System be stored?

FAQ ID - 3657

View

Which downstream applications have been tested with ccfDNA purified from whole blood collected and processed with the PAXgene Blood ccfDNA System?

FAQ ID - 3658

View

What is the shelf life of the QIAsymphony PAXgene Blood ccfDNA Kit?

FAQ ID - 3651

View

Which elution volumes are available with the QIAsymphony PAXgene Blood ccfDNA Kit?

FAQ ID - 3655

View

Why is a blood collection set required to collect blood into the PAXgene Blood ccfDNA Tube?

FAQ ID - 3628

View

Can reagent from the PAXgene Blood ccfDNA Tube flow back into the patient’s arm?

FAQ ID - 3629

View

Can I use a different brand blood collection set with the PAXgene Blood ccfDNA Tube?

FAQ ID - 3630

View

What is the blood draw volume of the PAXgene Blood ccfDNA Tube

FAQ ID - 3631

View

What is the difference between RT² Profiler PCR Array and RT² Profiler PCR Array PLUS version?

FAQ ID - 3685

View

Where can I find the gene list for a specific RT² Profiler PCR Array?

FAQ ID - 3684

View

Is it possible to store nucleic acids extracted using the SpeedXtract Virus Kit?

FAQ ID - 3680

View

Total RNA from what sample types are compatible with the QIAseq miRNA Library Kit?

FAQ ID - 3660

View

Can miRNA sequencing libraries be prepared from total RNA isolated from heparinized plasma samples?

FAQ ID - 3661

View

Are any components of the QIAseq miRNA Library Kit interchangeable with components from other QIAGEN kits?

FAQ ID - 3662

View

Can I still perform sequencing if my miRNA sequencing library concentrations are too low to obtain a 4 nM library?

FAQ ID - 3666

View

What method is used to eliminate adapter dimers from the QIAseq miRNA library?

FAQ ID - 3667

View

Does a QIAseq miRNA library include hY4 Y RNA?

FAQ ID - 3670

View

What is the sequence of the QIAseq miRNA NGS 3’ Adapter?

FAQ ID - 3671

View

Do the covered regions in the BED file include the primers?

FAQ ID - 3689

View

What do the GTIN, UDI and REF abbreviations mean on the label of any QIAGEN box?

FAQ ID - 3697

View

Do eluates generated with the PAXgene Blood miRNA Kit contain residual genomic DNA?

FAQ ID - 3637

View

Do you have a protocol for high-throughput purification of plasmid DNA using the BioRobot Universal System?

FAQ ID -1191

View

Is the surface tension of the crystallization supports of the EasyXtal 15-Well Tools similar to that of a siliconized cover slide?

FAQ ID -1107

View

Is the pH indicated in the formulation sheet for Screening Suite buffers the final pH?

FAQ ID -1123

View

What are the applications the EasyXtal 15-Well Tool can be used for?

FAQ ID -1111

View

Is the EasyXtal Tool compatible with liquid-handling systems and visualization robots?

FAQ ID -1118

View

Is it possible to collect intact mitochondria before or after the density gradient centrifugation step of the Qproteome Mitochondria Isolation protocol?

FAQ ID -1187

View

What cell lines have been tested with the Fastlane Cell cDNA Kit?

FAQ ID -1175

View

What does 'QIAGEN Certified Solution' for Protein Crystallization mean?

FAQ ID -1121

View

How many protein crystallization conditions are available at QIAGEN?

FAQ ID -1119

View

How much detection reagent do you generally recommend for LiquiChip Assays?

FAQ ID -1159

View

How many cells can be used with the Qproteome Mitochondria Isolation Kit, and how much mitochondrial protein can be expected?

FAQ ID -1189

View

How many drops can be set up on a protein crystallization support?

FAQ ID -1110

View

How many LiquiChip beads are recommended for an xMAP experiment?

FAQ ID -1161

View

How much solution can I add to an EasyXtal 15-Well Tool reservoir well?

FAQ ID -1114

View

In which formats are crystallization condition Screening Suites available?

FAQ ID -1127

View

If I cannot find a QuantiTect Primer Assay for my target gene in rat, can I use the assays for the orthologous genes in mouse and human?

FAQ ID -1145

View

If a QuantiTect Primer Assay has more than one version number, does this mean that earlier assay versions may not work?

FAQ ID -1136

View

How sensitive is the LiquiChip Reader?

FAQ ID -1157

View

How much time is needed for a LiquiChip assay point measurement?

FAQ ID -1162

View

Why are there various different QuantiTect Primer Assays for my gene (previous versions, transcript variants)?

FAQ ID -1133

View

What is the delivery time for QuantiTect Primer Assays?

FAQ ID -1142

View

Where can I find the composition tables for solutions used in individual Crystallization Screening Suites?

FAQ ID -1124

View

What kind of strategy for initial screening of protein crystallization conditions do you recommend?

FAQ ID -1120

View

When searching for a QuantiTect Primer Assay for my gene of interest, why do I only find the assay for human but not for rat?

FAQ ID -1139

View

What type of magnet do you recommend for handling the magnetic beads in the Qproteome Plasma Membrane Protein protocol?

FAQ ID -1193

View

What starting material can be used with the Qproteome Mitochondria Isolation Kit?

FAQ ID -1184

View

Can the EZ1 DNA Investigator Tip Dance protocol cope with any solid matrix remaining in the sample tube?

FAQ ID -1153

View

Are EasyXtal 15-Well Tools stackable?

FAQ ID -1116

View

Why can I not find the QuantiTect Primer Assay in GeneGlobe that I had previously ordered from QIAGEN?

FAQ ID -1138

View

Which QuantiTect Primer Assay should I choose for my gene of interest?

FAQ ID -1135

View

Why does the QuantiTect Primer Assay for my gene of interest have only one version number?

FAQ ID -1134

View

Which chemical products do you use to prepare screening solutions for protein crystallization?

FAQ ID -1122

View

Will proteins eluted in the Qproteome Plasma Membrane Protein protocol be modified, e.g. with the ligand?

FAQ ID -1195

View

Will the ligand in the Qproteome Plasma Membrane Protein protocol be specific for plasma membrane exclusively?

FAQ ID -1198

View

Will integral, peripheral as well as lipid-anchored plasma membrane proteins be isolated with the Qproteome Plasma Membrane Protein Kit?

FAQ ID -1199

View

Why is there no QuantiTect Primer Assay for my gene of interest?

FAQ ID -1140

View

What is the EasyXtal 15-Well Tool made of?

FAQ ID -1106

View

What is the dynamic range of detection of the LiquiChip Reader?

FAQ ID -1158

View

Can the nuclei and cell debris fraction generated in the QProteome Mitochondria isolation procedure be used for further fractionation?

FAQ ID -1185

View

Can the EZ1 DNA Forensic Card be used with the EZ1 DNA Investigator Kit?

FAQ ID -1151

View

Can the EasyXtal 15-Well Tools be reused?

FAQ ID -1117

View

Can the Qproteome Plasma Membrane Protein Kit be used with suspension cells?

FAQ ID -1194

View

Can your Tag antibody recognize rat MAPK1 also?

-100

View

Can xMAP kits from other Luminex partners be used on the LiquiChip System purchased from QIAGEN?

FAQ ID -1156

View

Can the remaining fractions obtained in the Qproteome Plasma Membrane Protein procedure be used in downstream assays?

FAQ ID -1197

View

Can the Spin Columns of the Qproteome Albumin/IgG Depletion Kit be reused?

FAQ ID -1173

View

Are scoring sheets available for each crystallization condition Screening Suite?

FAQ ID -1125

View

Can mitochondria separated with the Qproteome Mitochondria Isolation Kit be used for downstream DNA isolation?

FAQ ID -1188

View

Are protein modifications such as glycosylation problematic when using the Qproteome Plasma Membrane Protein Kit?

FAQ ID -1196

View

Can acetone be used for precipitation of protein from Buffer RLT lysates generated with RNeasy Kits?

FAQ ID -1164

View

Can I increase the annealing temperature recommended for QuantiTect Primer Assays used with the QuantiTect SYBR Green PCR Kits?

FAQ ID -1146

View

How are NeXtal deep-well blocks sealed? Can they be resealed?

FAQ ID -1128

View

Does the EasyXtal Tool conform to an SBS format?

FAQ ID -1115

View

Do you have WGA protocols for starting materials not mentioned in the REPLI-g Handbooks?

FAQ ID -1160

View

How easy is it to scoop up a protein crystal with the lip around the crystallization supports of the EasyXtal 15-Well Tools?

FAQ ID -1113

View

Do you have a protocol for the purification of 6xHis-tagged proteins using BioSprint?

FAQ ID -1167

View

How did you determine that your AllStar Negative Control siRNA binds to RISC?

FAQ ID -1180

View

How is the quality of the crystallization condition Screening Suites guaranteed?

FAQ ID -1126

View

How do I use EasyXtal Crystallization Tools?

FAQ ID -1105

View

How does the sealing efficiency of the crystallization supports of the EasyXtal 15-Well Tool compare to grease?

FAQ ID -1109

View

Do you have a kit for protein isolation from mitochondria prepared with your Qproteome Mitochondria Isolation Kit?

FAQ ID -1190

View

Do you have a kit for the isolation of RNA from formalin-fixed, paraffin-embedded samples?

FAQ ID -1174

View

Do I need an ultracentrifuge for the density gradient centrifugation step in the Qproteome Mitochondria Isolation protocol?

FAQ ID -1186

View

Do protein crystals stick to the walls of cavities when using the DropGuard crystallization supports?

FAQ ID -1108

View

Do you have a protocol for evaluating pipetting accuracy of the BioRobot EZ1?

FAQ ID -1131

View

Do you have a protocol for purification of DNA from stool samples for pathogen detection using BioSprint Instruments?

FAQ ID -1165

View

Do you have a protocol for purification of DNA from stool samples using BioSprint Instruments?

FAQ ID -1166

View

Do you have a protocol for evaluating temperature accuracy of the BioRobot EZ1?

FAQ ID -1168

View

Which competent E. coli cells should I use to amplify the SureSilencing shRNA Plasmids?

FAQ ID -2890

View

Which analyses can be performed with the PyroMark Q96 ID software version 1.0?

FAQ ID -2845

View

Does the same U1 promoter in the SureSilencing shRNA Plasmids express shRNA in human, mouse, and rat cell lines?

FAQ ID -2887

View

How can I use the SureSilencing shRNA Plasmids for stable transfection and knock down in a cell line that is already resistant to both neomycin and puromycin?

FAQ ID -2895

View

Can I use my own siRNA design?

FAQ ID -2897

View

Can I order the nucleotides from PyroMark Gold Reagents separately?

FAQ ID -2827

View

Can I reclone the insert sequences from the SureSilencing shRNA Plasmids into a viral-based vector?

FAQ ID -2896

View

Can I directly transfect SureSilencing shRNA Plasmids into my cells?

FAQ ID -2899

View

Do any of the SureSilencing shRNA Plasmids contain inducible promoters?

FAQ ID -2888

View

Can PyroMark Gold reagents be vortexed?

FAQ ID -2844

View

What are the features of the new PyroMark Q96 ID software 2.5?

FAQ ID -2867

View

What cell line does QIAGEN use for quality control testing of the SureSilencing shRNA Plasmids?

FAQ ID -2885

View

Should I use the SureSilencing shRNA Plasmids with the neomycin-resistance marker, the puromycin-resistance marker, or the GFP reporter gene?

FAQ ID -2882

View

What else do I need to complete an experiment using the SureSilencing shRNA Plasmids?

FAQ ID -2889

View

What cell lines can I use with the SureSilencing shRNA Plasmids?

FAQ ID -2891

View

How long should I wait after transfection before enriching my transfected cells, validating knock down, and looking for phenotypic changes?

FAQ ID -2893

View

Is the expression level of miRNA precursors lower compared to that of mature miRNAs?

FAQ ID -2807

View

How many times can the CDTs, NDTs, and RDTs be used?

FAQ ID -2865

View

What is the PyroMark Q96 Data Converter?

FAQ ID -2868

View

What is the pyrosequencing data exchange tool for?

FAQ ID -2864

View

What is the composition of Buffer APP?

FAQ ID -2804

View

What is the composition of Buffer FRN?

FAQ ID -2802

View

What is the composition of Buffer APL?

FAQ ID -2803

View

Which analyses can be performed with the PyroMark Q96 MD software?

FAQ ID -2866

View

Which PyroMark Gold Q96 Reagent should be used for which instrument and application?

FAQ ID -2836

View

What is the SureSilencing shRNA Plasmids guarantee?

FAQ ID -2898

View

What primers may I use to sequence the shRNA cassette within the SureSilencing shRNA Plasmids?

FAQ ID -2886

View

Does the PyroMark CpG LINE assay target mouse transposons as well?

-1

View

How do I find the SureSilencing shRNA Plasmids for my gene of interest?

FAQ ID -2883

View

How are the SureSilencing shRNA Plasmids shipped?

FAQ ID -2884

View

What is the concentration of SureENTRY Transduction Reagent?

FAQ ID - 3708

View

Is it possible to get a Custom RT2 Profiler PCR Array, cat. # 330131, with primer assays targeting transcripts of different species, e.g. assays targeting transcripts from mouse and assays targeting transcripts from human, on one plate?

FAQ ID - 3710

View

Is it possible to purchase Buffer VXL which is part of the QIAamp cador Pathogen Mini Kit, (Cat No.: 54104, 54106) separately?

FAQ ID - 3712

View

What sample tubes can I use on my QIAsymphony SP system?

FAQ ID - 3713

View

How can I decontaminate my QIAsymphony SP / AS system?

FAQ ID - 3714

View

Can other QIAGEN buffers from non-UCP kits (e.g. AW1 instead of AUW1) be used with the QIAamp UCP DNA Micro Kit (cat. no. 56204)?

FAQ ID - 3717

View

How is the Pyromark Q48 cartridge to be cleaned?

FAQ ID - 3718

View

Can the disc and absorber strips for the Pyromark Q48 be reused?

FAQ ID - 3720

View

What is the dead volume of the cartridges in PyroMark Q48?

FAQ ID - 3719

View

How much DNA is needed in the PyroMark Q48?

FAQ ID - 3721

View

Can I use PyroMark Q48 for diagnostics?

FAQ ID - 3722

View

Can I transfer my assays from PyroMark Q24/Q96 to PyroMark Q48 Autoprep?

FAQ ID - 3723

View

Can I analyze my Next Generation Sequencing (NGS) samples on the QIAxcel for Quality control?

FAQ ID - 3724

View

Is an additional proteinase K digest possible with DNeasy PowerSoil Kit and DNeasy PowerLyzer Kit?

FAQ ID - 3725

View

Can I buy the 1 ml Bead Plates from the DNeasy UltraClean 96 Microbial Kit (384) separately?

FAQ ID - 3726

View

Does QIAGEN offer an anti-GFP gapmer control?

FAQ ID - 3739

View

Why do I observe a CT shift for the Internal Control in some samples in comparison to the CT value for the Internal Control of the dilution series of the Male Control DNA M1?

FAQ ID - 3745

View

How long can I store the dilutions of the Male Control DNA M1 standards at 2–8°C?

FAQ ID - 3744

View

Do I need to buy special kits for the QIAcube Connect?

FAQ ID - 141485

View

Can I program my own protocols for the QIAcube Connect?

FAQ ID - 141486

View

Can the QIAcube Connect rotor and/or buckets be removed for cleaning?

FAQ ID - 141491

View

Can purification columns from other suppliers be processed on the QIAcube Connect?

FAQ ID - 141487

View

How can I get software updates for the QIAcube Connect?

FAQ ID - 141494

View

Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube Connect?

FAQ ID - 141496

View

Do I need to discard partially used QIAcube Connect tip racks?

FAQ ID - 141499

View

How can I decontaminate the QIAcube Connect system?

FAQ ID - 141500

View

Can the QIAcube Connect heater/shaker be used independently from protocol runs?

FAQ ID - 141502

View

How can I load new protocols onto the QIAcube Connect?

FAQ ID - 141501

View

What dedicated QIAcube Connect kits are available?

FAQ ID - 141504

View

Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube Connect?

FAQ ID - 1414507

View

Where can I find the ordering information for QIAcube Connect accessories such as shaker rack plugs, rack labeling strips, reagent bottle racks, 30 ml reagent bottles, rotor-adapter holder, rotor adapters, sample tubes CB, sample tubes RB and spin-column adapter rings?

FAQ ID - 141505

View

Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube Connect?

FAQ ID - 141506

View

Which QIAcube Connect standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet?

FAQ ID - 141508

View

How often should the o-ring for the pipettor tip adapter be changed on the QIAcube Connect?

FAQ ID - 141509

View

Can the CB and RB sample tubes be used interchangeably?

FAQ ID - 141510

View

Which network settings are supported by the QIAcube Connect and Q-Base?

FAQ ID - 141517

View

Why can I not find the Q-Base device in the device list during the first installation?

FAQ ID - 141516

View

Why can’t I see my QIAcube Connect instrument in the QIAcube Connect App?

FAQ ID - 1414518

View

Why is the QIAcube Connect App reacting so slowly?

FAQ ID - 141519

View

Why are not all applications/kits/protocol files visible in the run setup (QIAcube Connect App)?

FAQ ID - 1414520

View

Why does the connection test fail?

FAQ ID - 141522

View

How can I accurately determine the amount of input material for the EpiTect Hi-C Kit?

FAQ ID - 143065

View

What sample source do you support?

FAQ ID - 143066

View

How long does it take to complete the EpiTect Hi-C protocol?

FAQ ID - 143068

View

How many reactions are in a kit?

FAQ ID - 143067

View

Can I use cells that have been previously crosslinked and frozen (e.g., for a ChIP or ChIP-seq experiment) as input material for the EpiTect Hi-C protocol?

FAQ ID - 143069

View

Can I substitute the endonuclease used in the Hi-C Digestion step with another endonuclease?

FAQ ID - 143070

View

Are there stopping points in the Hi-C workflow?

FAQ ID - 143071

View

With which platform can I perform my sequencing?

FAQ ID - 143072

View

With which read length should the EpiTect Hi-C NGS libraries be sequenced

FAQ ID - 143073

View

How do I know if my Hi-C sample is a good candidate for deep sequencing?

FAQ ID - 143074

View

What kind of analysis does the EpiTect Hi-C Data Analysis Portal perform?

FAQ ID - 143076

View

Can I use my own pipeline to analyze results from my EpiTect Hi-C experiments?

FAQ ID - 143078

View

How critical is the time of the temperature change from 60°C to 80°C?

FAQ ID - 143768

View

Is it possible to use Lyse&Spin baskets?

FAQ ID - 143762

View

Why is DTT used only for semen samples?

FAQ ID - 143761

View

Can the Repli-g Advanced DNA Single Cell Kit also be used for bacterial cells?

FAQ ID - 143079

View

Can the lysis volume be reduced for higher sensitivity?

FAQ ID - 143763

View

What if only one heater shaker is available?

FAQ ID - 143764

View

Why is there no male DNA obtained from sexual assault samples?

FAQ ID - 143767

View

If 400 µl are used as lysis volume, how do I concentrate the lysate? (Otherwise the DNA would be too diluted.)

FAQ ID - 143766

View

Why is the QIAamp Viral RNA Mini Kit (50) currently unavailable?

FAQ ID -147395

View

What RNAs has QIAseq FastSelect been designed to remove?

FAQ ID -147910

View

How does QIAseq FastSelect work?

FAQ ID -147907

View

Is QIAseq FastSelect truly as easy as combining a reagent with RNA and ramping down the temperature?

FAQ ID -147909

View

Will QIAseq FastSelect work in other species?

FAQ ID -147911

View

What library prep kits has QIAseq FastSelect been tested with?

FAQ ID -147912

View

I am using an RNA library prep kit that is not listed in the QIAseq FastSelect handbook. Will QIAseq FastSelect work with my kit?

FAQ ID -147913

View

What RNA range has the QIAseq FastSelect been tested with?

FAQ ID -147914

View

Can QIAseq FastSelect Plant or QIAseq FastSelect Yeast be combined with FastSelect 5S/16S/23S?

FAQ ID -147915

View

Is it possible to test the efficiency of FastSelect rRNA removal by using a Bioanalyzer, etc.?

FAQ ID -147916

View

Is plasmid DNA purified with QIAGEN Plasmid Purification Kits suitable for in vitro transcription?

FAQ ID -1

View

How should I quantify RNA isolated with RNeasy Kits?

FAQ ID -32

View

When is chloramphenicol amplification of plasmids performed?

FAQ ID -3

View

What are the expected DNA yields from different tissues using the QIAamp DNA Mini Kit?

FAQ ID -45

View

What are the most commonly used protease inhibitors?

FAQ ID -53

View

How should I propagate pQE expression plasmids?

FAQ ID -58

View

How can I increase the amount of soluble recombinant protein in E. coli expression?

FAQ ID -64

View

How can I remove imidazole from a protein sample?

FAQ ID -91

View

Why does my isolated RNA have a low OD 260/280 ratio?

FAQ ID -97

View

How can I avoid little or no RNA yields when using an RNeasy Kit?

FAQ ID -28

View

Do I need to use an RNase inhibitor in my RT reaction?

FAQ ID -119

View

Can I use QIAprep Miniprep kits for low-copy plasmids and cosmids?

FAQ ID -127

View

Can I use the QIAquick PCR Purification Kit for restriction enzyme cleanup?

FAQ ID -130

View

How does imidazole affect my quantitation of protein?

FAQ ID -132

View

Can a QIAquick Gel Extraction Kit be used to obtain RNA from a formaldehyde gel?

FAQ ID -133

View

What is the difference between disruption and homogenization in the RNeasy System?

FAQ ID -139

View

What is the size of genomic DNA that is obtained with QIAGEN Genomic-tips?

FAQ ID -142

View

Should I use Ni-NTA Agarose in column or batch format for purification of 6xHis-tagged proteins?

FAQ ID -147

View

What is the genotype of the EZ Competent Cells?

FAQ ID -157

View

What are the restriction sites of the pDrive Vector in the QIAGEN PCR Cloning Kit?

FAQ ID -160

View

What are the molecular weights of proteins in the 6xHis Protein Ladder?

FAQ ID -169

View

How can I optimize the transfection of oligos and large plasmids using PolyFect Transfection Reagent?

FAQ ID -176

View

How can I improve recoveries when using the QIAquick Kits?

FAQ ID -180

View

How can I improve transfection efficiency using Effectene Transfection Reagent?

FAQ ID -181

View

What is the principle behind Effectene Transfection Reagent?

FAQ ID -184

View

What is the composition of Buffer EB?

FAQ ID -199

View

Is it possible to modify the transduction protocol for Cignal Lenti Reporter Assays to save some pipetting steps?

FAQ ID - 3709

View

Can I store the QIAseq Beads of the QIAseq Targeted DNA Panel at -20°C?

FAQ ID - 3711

View

What is the difference between the QIAamp UCP DNA Micro Kit (cat. no. 56204) and RNeasy UCP Kits (cat. no. 73934) and non-UCP QIAGEN purification kits?

FAQ ID - 3715

View

Can I use carrier RNA with the RNeasy UCP Micro Kit (cat. no. 73934)?

FAQ ID - 3716

View

How can I check for purity of RNA isolated using RNeasy Kits?

FAQ ID -1023

View

What is the recipe for 1x PBS solution?

FAQ ID -1030

View

Where can I find a protocol for cleanup of already purified plasmid DNA?

FAQ ID -1031

View

How do I perform an Acetone Precipitation for concentrating and desalting protein samples?

FAQ ID -1035

View

What is the difference between Buffers RLT and RLT Plus?

FAQ ID -1043

View

What is the largest PCR amplicon that can be amplified with the HotStar HiFidelity Polymerase Kit?

FAQ ID -1047

View

Are the CoralLoad dyes in the HotStarTaq Plus PCR Buffer visible when loading small amounts of PCR product onto a gel?

FAQ ID -1051

View

Do I have to use CoralLoad Gel loading dye when using your HotStarTaq Plus DNA Polymerase?

FAQ ID -1052

View

How can I increase DNA concentration using QIAprecipitators of the HiSpeed Plasmid Kits?

FAQ ID -1061

View

What should I use as a standard for absolute quantification in real-time PCR?

FAQ ID -1085

View

What does transcript variant mean when searching for specific QuantiTect Primer Assays?

FAQ ID -1137

View

What is a QuantiTect Primer Assay?

FAQ ID -1141

View

Do QuantiTect Primer Assays contain SYBR Green dye?

FAQ ID -1143

View

What is the smallest and the largest protein that you tested for expression with the EasyXpress Insect Kit II?

FAQ ID -1218

View

What are the new features of the EasyXpress Insect Kit II compared to the original Protein Synthesis Insect kit?

FAQ ID -1220

View

What are your recommendations for PCR template preparation for use with the EasyXpress Insect Kit II?

FAQ ID -1221

View

Do you have a protocol for transfection of suspension cell lines (Jurkat and K562) with siRNA?

FAQ ID -1250

View

Do you have a protocol for transfection of macrophage cell lines (J774.A1 and RAW 264.7) with siRNA?

FAQ ID -1251

View

What is the pH value of QIAGEN's Nuclease-Free Water?

FAQ ID -1290

View

Why is there DNA in the no-template control reaction when using the standard REPLI-g procedure, but not when using the UltraFast procedure?

FAQ ID -1327

View

Will the REPLI-g UltraFast Mini Kit reaction always yield 10 µg DNA?

FAQ ID -1328

View

Can the bacterial pellet in the QuickLyse Miniprep procedure be resuspended by pipetting up and down or shortening the vortexing time?

FAQ ID -1352

View

How can I increase the DNA yield from low-copy plasmids using the QuickLyse Miniprep Kit?

FAQ ID -1354

View

What is the composition of elution buffer QLE in the QuickLyse Miniprep Kit?

FAQ ID -1356

View

How do I resuspend my FlexiPlate siRNAs?

FAQ ID -1360

View

Are there any specific cleaning solutions recommended for the QIAcube?

FAQ ID -1416

View

Why is a 2-step (and not a 3-step) cycling protocol recommended for QuantiFast SYBR Green PCR Kits?

FAQ ID -1450

View

Can tissue homogenized in Buffer RLT Plus instead of Buffer RLT be used with the AllPrep DNA/RNA/Protein Mini Kit?

FAQ ID -1577

View

What can be done if the protein pellet does not solubilize completely using the AllPrep DNA/RNA/Protein Mini Kit?

FAQ ID -1580

View

What is the binding capacity of the Ni-NTA Superflow Cartridges?

FAQ ID -1603

View

How comparable is CoralLoad gel loading dye contained in various QIAGEN PCR Kits to Sigma Red?

FAQ ID -1644

View

Do I need to resuspend FlexiTube siRNAs in siRNA Resuspension buffer?

FAQ ID -1659

View

Are FlexiTube siRNA and FlexiTube GeneSolution also available for rat siRNAs?

FAQ ID -1664

View

What is the composition of Buffer PE?

FAQ ID -1691

View

What is the upper length of PCR products that can be amplified with TopTaq DNA Polymerase and Master Mix Kits?

FAQ ID -1738

View

What is the minimum sample volume required for the QIAxcel System and QIAxcel Advanced?

FAQ ID -1852

View

What are the dimensions and weight of the QIAsymphony SP System?

FAQ ID -1901

View

Are there any specific cleaning solutions recommended for the QIAsymphony SP?

FAQ ID -1913

View

How much sample can be used with the RNeasy Plus 96 Kit?

FAQ ID -1992

View

What is the composition of Buffer P2?

FAQ ID -203

View

A white precipitate has formed in my 10x RT buffer. Is it still ok to use?

FAQ ID -216

View

For how long are BioMag Particles stable?

FAQ ID -262

View

I used to buy BioMag particles from a company by another name? Are your BioMag particles the same thing?

FAQ ID -263

View

Can I clean up my DNase treated RNA samples using RNeasy columns?

FAQ ID -286

View

How can one determine the optimal annealing temperature for a specific PCR assay?

FAQ ID -288

View

What is the composition of the QIAGEN Multiplex PCR Buffer?

FAQ ID -289

View

Can I use HEPES buffer instead of phosphate in my Ni-NTA column?

FAQ ID -291

View

Can I use a centrifuge instead of vacuum when using the QIAquick 96 PCR Purification Kit?

FAQ ID -293

View

What are the ideal and the largest PCR amplicon sizes when using the EpiTect MSP Kit?

FAQ ID -2004

View

What is elution Buffer ATE of the QIAsymphony DNA Investigator Kit?

FAQ ID -2029

View

How can a C-terminal 6xHis-tagged QIAgene be expressed?

FAQ ID -2041

View

Why is there more than one QIAgene for my gene of interest?

FAQ ID -2044

View

Is a passive internal reference dye, like ROX, required on the Rotor-Gene Q to obtain reproducible results?

FAQ ID -2079

View

Does the Rotor-Gene Q software have the capacity to export data?

FAQ ID -2089

View

Why is a 2-step (and not a 3-step) cycling protocol recommended for Rotor-Gene SYBR Green Kits?

FAQ ID -2122

View

Does carrier RNA have to be added using the QIAsymphony Virus Blood 200 Protocol?

FAQ ID -2163

View

Can I use the RNeasy Microarray Tissue Mini Kit for all tissue types?

FAQ ID -2190

View

How is the RNeasy Microarray Tissue Mini Kit different from the classic RNeasy Mini Kit?

FAQ ID -2192

View

Why is EvaGreen instead of SYBR Green used as fluorescent dye in the Type-it HRM PCR Kit?

FAQ ID -2196

View

Why do I need normalization using Rotor-Gene ScreenClust HRM Software?

FAQ ID -2198

View

What is the difference between probability and typicality in the Rotor-Gene ScreenClust HRM Software?

FAQ ID -2203

View

What buffer conditions give the best resolution for agarose gel electrophoresis?

FAQ ID -2256

View

How much DNA should be loaded per well of an agarose gel?

FAQ ID -2259

View

Why do I have wavy DNA bands on my agarose gel?

FAQ ID -2260

View

What is the "seed sequence" of a miRNA?

FAQ ID -2262

View

Are you able to supply a Certificate of Analysis for the KRAS and EGFR PCR kits?

FAQ ID -2301

View

Which Rotor-Gene Q rotor should be used with the EGFR RGQ PCR kit?

FAQ ID -2315

View

How is the RNeasy Plus Universal Kit different from the RNeasy Plus Kit?

FAQ ID -2340

View

How does the gDNA eliminator solution of the RNeasy Plus Universal Kit work?

FAQ ID -2341

View

How long can I store my purified DNA/RNA isolated with the AllPrep DNA/RNA FFPE kit?

FAQ ID -2350

View

Do you have any information or guidelines regarding the choice of reference genes for real-time PCR?

FAQ ID -2371

View

Can I buy QIAquick and MinElute columns separately?

FAQ ID -2460

View

What is the PAXgene 96 filter plate?

FAQ ID -2486

View

Is RNA from red blood cells also isolated when RNA is extracted from a PAXgene Bone Marrow RNA Tube?

FAQ ID -2509

View

Is the 260/230 nm absorbance ratio specified for RNA derived from the PAXgene Bone Marrow RNA System?

FAQ ID -2514

View

How long is the fixation time?

FAQ ID -2519

View

What is the nature of the heterologous Internal Amplification Control and Positive Control in the mericon Assay kits?

FAQ ID -2543

View

Are there any accessories required for sample disruption with the TissueLyser LT?

FAQ ID -2551

View

Why do the Internal Control templates for extraction (Internal Control DNA or RNA [High conc.]) have a 10x higher concentration than the IC templates provided with the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit?

FAQ ID -2603

View

During data analysis, how should the threshold be set in the Yellow channel on the Rotor-Gene Q cycler to analyze the Internal Control from the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit

FAQ ID -2607

View

What is the SBTengine® software?

FAQ ID -2628

View

How do I create a workspace that is free of DNA contamination, prior to carrying out a qPCR experiment?

FAQ ID -2654

View

What is the recommended solution in which to store RNA samples that will be used as templates for cDNA synthesis?

FAQ ID -2659

View

How can I avoid or remove genomic DNA contamination from the total RNA preparation?

FAQ ID -2662

View

What are the differences between one-step and two-step RT--PCR?

FAQ ID -2666

View

What is qPCR?

FAQ ID -2668

View

What are the common primer and probe chemistries utilized for qPCR assays?

FAQ ID -2669

View

What is the purpose of the ROX and fluorescein dyes, also known as passive reference dyes, in qPCR master mixes?

FAQ ID -2671

View

What negative controls are typically included in qPCR and/or qRT-PCR experiments?

FAQ ID -2672

View

What positive controls are typically included in qPCR and/or qRT-PCR experiments?

FAQ ID -2673

View

What are the guidelines for choosing a housekeeping gene for normalizing qPCR results?

FAQ ID -2674

View

Why is 18S ribosomal RNA (rRNA) used as a housekeeping gene to normalize sample-to-sample, systematic variation in qPCR assays?

FAQ ID -2675

View

How does HotStart PCR help minimize nonspecific amplification events?

FAQ ID -2676

View

What is a dissociation curve, and why is it important to run a dissociation curve, following qPCR using SYBR Green chemistry?

FAQ ID -2678

View

What is the delta Rn value?

FAQ ID -2681

View

What is the threshold cycle or Ct value?

FAQ ID -2682

View

Why are my qPCR Ct values too low (< 12) in my qRT-PCR Assay?

FAQ ID -2684

View

Why are my qPCR Ct values too high (> 35 or not detectable) in my qRT-PCR assay?

FAQ ID -2685

View

Why is my no template control (NTC) real-time Ct value < 35 cycles in my qPCR Assay?

FAQ ID -2686

View

Why do my qPCR amplification curves or plots decrease in fluorescence intensity after the saturation phase?

FAQ ID -2689

View

Why do I see multiple high-intensity peaks in my qPCR dissociation curve at temperatures less than 70 ºC?

FAQ ID -2690

View

What is the standard curve method for qPCR assay data analysis? How is the standard curve method for qPCR assay data analysis performed?

FAQ ID -2691

View

What is the difference between Absolute Quantification and Relative Quantification in qPCR, using the standard curve approach?

FAQ ID -2692

View

What is the comparative or ??Ct method for qPCR assay data analysis? How is the comparative or ??Ct method for qPCR assay data analysis performed?

FAQ ID -2693

View

How do I determine the amplification efficiency of my qPCR assay?

FAQ ID -2694

View

How do I determine the linear dynamic range of my qPCR or qRT-PCR assay?

FAQ ID -2696

View

What is the best approach for determining where to set the CT threshold when you have >15 samples? Is it best to go through all of them, looking for a range of best fit, and then just choose one value that fits all of them?

FAQ ID -2705

View

What do I need to complete a RT² qPCR Primer Assay?

FAQ ID -2707

View

How can I prevent the evaporation of reaction volume from the wells in the miScript miRNA PCR array?

FAQ ID -2729

View

What is the minimum amount of genomic DNA required for analysis using EpiTect Methyl qPCR Arrays?

FAQ ID -2739

View

How do you design your EpiTect ChIP qPCR Assay primers? Do they work with SB GR?

FAQ ID -2749

View

How can I identify the binding sites of my transcription factor using ChIP?

FAQ ID -2753

View

At what point can I stop and freeze my samples when using ChIP?

FAQ ID -2754

View

What is RNA interference (RNAi)?

FAQ ID -2755

View

What are the key factors for success in an RNAi experiment?

FAQ ID -2758

View

What criteria should one use in choosing between siRNA versus shRNA for their studies?

FAQ ID -2771

View

How does stable transfection of a shRNA-encoding plasmid work?

FAQ ID -2779

View

What are the SureSilencing shRNA Plasmids?

FAQ ID -2784

View

What is the composition of Buffer S3?

FAQ ID -2788

View

What is the composition of Buffer ETR?

FAQ ID -2789

View

What is the composition of Buffer BB?

FAQ ID -2790

View

What is the composition of Buffer RLT plus?

FAQ ID -2794

View

What is the composition of Buffer RLC?

FAQ ID -2795

View

What is the composition of Buffer RPE?

FAQ ID -2797

View

What is the composition of Buffer RWT?

FAQ ID -2798

View

What is the composition of gDNA Eliminator Solution?

FAQ ID -2799

View

What is the composition of Buffer RDD?

FAQ ID -2800

View

What is the composition of Buffer PKD?

FAQ ID -2801

View

What is the composition of Buffer ALO?

FAQ ID -2805

View

What is the composition of RBC Lysis Solution?

FAQ ID -2808

View

What is the composition of Cell Lysis Solution?

FAQ ID -2809

View

What is the composition of Protein Precipitation Solution?

FAQ ID -2810

View

What is the composition of Cell Suspension Solution?

FAQ ID -2811

View

What is the composition and concentration of Glycogen Solution in Gentra Puregene Kits?

FAQ ID -2812

View

What is the composition of DNA Hydration Solution in Gentra Puregene Kits?

FAQ ID -2813

View

What is the meaning of the abbreviations NDT and CDT and what are the differences?

FAQ ID -2841

View

What are the changes of PyroMark Assay Design Software Version 2.0 compared to 1.0?

FAQ ID -2849

View

Can I use fluorescence microscopy to assess the transfection efficiency of the SureSilencing shRNA Plasmids with GFP in my model cell line of interest?

FAQ ID -2894

View

Do tissue samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction?

FAQ ID -2926

View

What is the composition of Buffer G2?

FAQ ID -2943

View

What is the composition of Buffer B1?

FAQ ID -2944

View

What is the composition of Buffer B2?

FAQ ID -2945

View

How much DNA does a human cell contain?

FAQ ID -2948

View

How much RNA does a bacterial cell contain?

FAQ ID -2949

View

How much DNA and RNA can be expected from human blood cells?

FAQ ID -2950

View

What is the cellular composition of human blood?

FAQ ID -2951

View

How can I desalt and concentrate a protein fraction by trichloroacetic acid (TCA) precipitation?

FAQ ID -2954

View

Is there a risk of crosstalk from the FAM channel to the VIC channel if the residual DNA target is present in very high copy numbers?

FAQ ID -2980

View

Why is the final heat denaturation step so important with the QIAsymphony PAXgene Blood RNA protocol?

FAQ ID -2987

View

What is the sequence of the miScript Universal Primer?

FAQ ID -2994

View

How can I keep my centrifuge tubes endotoxin-free?

FAQ ID -301

View

Can Buffers N3 and P3 be used interchangeably?

FAQ ID -310

View

Are QIAprep and QIAquick Spin columns interchangeable?

FAQ ID -311

View

Can I store agarose gel slices containing DNA for gel extraction at a later point?

FAQ ID -313

View

How can QIAGEN Protease and Proteinase K be inactivated?

FAQ ID -315

View

Does the 5x OneStep RT-PCR Buffer contain BSA?

FAQ ID -326

View

What is the origin of replication and the plasmid copy number of the pQE vectors?

FAQ ID -338

View

Is QIAGEN carrier RNA sold separately?

FAQ ID -351

View

What is the white insoluble precipitate in my resuspended plasmid DNA pellet?

FAQ ID -352

View

What is the composition of PBS?

FAQ ID -361

View

How can I express toxic protein in E. coli?

FAQ ID -373

View

How can I improve DNA yields from very tough tissues using the DNeasy Blood & Tissue Kit or the QIAamp DNA Mini Kit?

FAQ ID -374

View

What are the dissociation constants for the Anti-His Antibodies?

FAQ ID -385

View

What is the average molecular weight of a siRNA, and how do I convert uM to ug values?

FAQ ID -388

View

Can siRNA silence bacterial genes?

FAQ ID -395

View

How should I reconstitute the lyophilized RNase-Free DNase Set with the RNeasy 96 kit?

FAQ ID -3003

View

Is it possible to use a standard processor — the kind used routinely for formalin-fixed samples — for dehydration and paraffin infiltration of PAXgene Tissue treated samples?

FAQ ID -3032

View

Can I use Tungsten Carbide beads for RNA extraction protocols using QIAGEN Kits?

FAQ ID -3053

View

Where can I find the ordering information for QIAcube accessories such as Shaker Rack plugs, Rack labeling strips, Reagent bottle rack, 30mL Reagent bottles, Rotor Adapter holder, Rotor adapters, Sample tubes CB, Sample tubes RB and Spin column adapter rings?

FAQ ID -3054

View

What is the part number for O-rings for QIAsymphony SP and AS instruments? How often should the O-rings be replaced?

FAQ ID -3055

View

What is the thermal output for a BioRobot MDx and BioRobot Universal?

FAQ ID -3056

View

What is the thermal output for a QIAsymphony SP and AS instrument?

FAQ ID -3057

View

How precise is the QIAgility?

FAQ ID -3063

View

What are the advantages and disadvantages of turning on the tip “re-use” feature on the QIAgility?

FAQ ID -3064

View

How long does it take to set up a 96-well plate PCR reaction on the QIAgility?

FAQ ID -3066

View

Can I use a bar code scanner or reader on the QIAgility?

FAQ ID -3067

View

How can I find the run parameters for a BioSprint protocol that I am running?

FAQ ID -3069

View

Can BioSprint 96 be connected to LIMS?

FAQ ID -3070

View

What is the expected level of endotoxins in plasmid DNA purified with QIAprep spin miniprep kit?

FAQ ID -3081

View

What is the expected level of endotoxins in plasmid DNA purified with QIAGEN Plasmid kits, QIAfilter Plasmid kits and HiSpeed Plasmid purification kits?

FAQ ID -3082

View

What is the concentration of the Gentra RNase A that is used with the Autopure and Puregene protocols?

FAQ ID -3083

View

What is the concentration of the different Proteinase K solutions sold by QIAGEN?

FAQ ID -3084

View

Where can I access Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit?

FAQ ID -3085

View

What tubes can be used with TissueLyser Adapter Set 2 x 96 (cat. no. 69984)?

FAQ ID -3086

View

How to upload a Flexiplate on QIAGEN Webpage?

FAQ ID -3103

View

Can we do modification XXXXX for custom siRNA that is not on the web page?

FAQ ID -3107

View

What is the stability of siRNA lyophilized and resuspended?

FAQ ID -3109

View

Do you offer FlexiTube or FlexiPlate siRNAs for rat?

FAQ ID -3114

View

Is it possible to extract RNA from frozen blood?

FAQ ID -3119

View

Is it possible to extract RNA from RNAprotect-stabilized-blood?

FAQ ID -3120

View

What's the pulsed-field gel electrophoresis (PFGE) conditions of the gel picture showing on your website? http://www.qiagen.com/Search/MagAttract-HMW-DNA-Kit#productdetails

-48

View

Can Dithiothreitol (DTT) be used instead of BME in Buffer RLT Plus of RNeasy Plus Mini Kit?

FAQ ID -3125

View

Why does my purified DNA/RNA sometimes have a 260/280 or 260/230 ratio of more than 2? Does that mean the purity is poor?

FAQ ID -3132

View

How do I remove the RNA contamination from purified DNA?

FAQ ID -3133

View

What is the final concentration of Protease upon reconstitution in the QIAamp DNA Blood Mini?

FAQ ID -3140

View

What is the Spike-in-Control in the miRNeasy Serum/Plasma Kit ?

FAQ ID -3169

View

What is the difference between the 1 nmol FlexiTube and FlexiTube Gene Solution siRNAs?

FAQ ID -3174

View

What could be a reason for cloudy precipitate in PCR reaction with STR Plus chemistry after cycling protocol is completed?

FAQ ID -3175

View

What is needed for performing delta delta Ct relative quantitation on the Rotor-Gene Q?

FAQ ID -3178

View

Is REST software Windows 7 compatible?

FAQ ID -3182

View

Can the Rotor-Gene Q perform gradient PCR?

FAQ ID -3183

View

What is the expected amplicon size of the Microbial DNA qPCR Assays?

FAQ ID — 3396

View

What is the difference between Positive PCR Control (PPC) and Microbial DNA Positive Control?

FAQ ID — 3397

View

What is LLOQ?

FAQ ID — 3401

View

What is the difference between LLOQ and LOD?

FAQ ID — 3402

View

What species are detected by the Pan Bacteria 1 and Pan Bacteria 3 Assays?

FAQ ID - 3427

View

What is the composition of Buffer AC in the QIAamp UltraSens Virus Kit?

FAQ ID - 3429

View

Can the miScript II RT Kit be used to quantify plant miRNAs?

FAQ ID - 3431

View

What is the dissociation curve temperature of PCR products generated with the miScript Plant qPCR system?

FAQ ID - 3444

View

Can specific types of white blood cells be enriched from a PAXgene Blood RNA Tube prior to the RNA isolation?

FAQ ID - 3467

View

What is the proper disposal procedure for waste from the sample preparation?

FAQ ID - 3470

View

What is the expected yield from 8.5 ml of blood?

FAQ ID - 3496

View

What does the resuspension buffer, Buffer BG4, in the PAXgene Blood DNA kit contain?

FAQ ID - 3501

View

How should blood samples drawn into PAXgene Blood DNA Tubes be transported?

FAQ ID - 3507

View

How long can samples in PAXgene Blood DNA Tubes be stored at room temperature?

FAQ ID - 3508

View

What is the expected DNA yield from 2.5 ml of blood when using the QIAsymphony, QIAcube, and salting-out precipitation method?

FAQ ID - 3510

View

Which downstream applications have been tested with DNA purified from samples collected in PAXgene Blood DNA Tubes IVD?

FAQ ID - 3512

View

What does PAXgene Tissue STABILIZER contain?

FAQ ID - 3513

View

What is the minimum number of cells that can be efficiently processed with the QIAamp DNA Mini and QIAamp Blood Mini kits and what is the expected yield?

FAQ ID - 3516

View

Is the quality and size of DNA extracted with the DNeasy Blood & Tissue kit good enough to generate DNA-libraries for next generation sequencing?

FAQ ID - 3517

View

What is the minimum number of cells or minimum amount of tissue that can be efficiently processed with the RNeasy mini kit?

FAQ ID - 3519

View

Can I use the RNeasy Fibrous Tissue Mini Kit (cat. no. 74704) to extract RNA from fibrous plant material?

FAQ ID - 3521

View

Will the Reverse transcription control on the RT2 profiler PCR array work on any cDNA library?

FAQ ID - 3534

View

Microscopic/visual confirmation of successfully sorted cells can be used to optimize sorting conditions. Can stained cells be used?

FAQ ID - 3559

View

Is the miRNA reverse transcription control (miRTC) used in conjunction with the miScript Single Cell qPCR Kit?

FAQ ID - 3573

View

Is clot detection possible on the QIAsymphony?

FAQ - ID 3588

View

How can I determine if a tube is suitable for clot detection on QIAsymphony?

FAQ ID - 3590

View

How should I thaw frozen plasma samples?

FAQ ID - 3645

View

How do Unique Molecular Indexes (UMIs) improve quantification?

FAQ ID - 3669

View

Can REPLI-g WGA be used to amplify cDNA if the cDNA is dsDNA (double-stranded DNA)?

FAQ ID - 3686

View

I would like to stop during the library preparation procedure. When can I do that?

FAQ ID - 3687

View

Which regions are covered by the GR DNAseq V2 Panels (#181900)?

FAQ ID - 3688

View

When preparing plasma with the PAXgene Blood ccfDNA tubes, cat. # 768115, do you recommend that the first centrifugation step should be done with the brakes on or off?

FAQ ID - 3690

View

How do I insert and replace an absorber strip into the PyroMark Q48 Autoprep chamber?

FAQ ID - 3691

View

Are peak heights of Q48 comparable to other PyroMark instruments?

FAQ ID - 3692

View

How much space does the PyroMark Q48 Autoprep instrument need?

FAQ ID - 3693

View

Can unused wells in a pyrosequencing discs be used in the next run?

FAQ ID - 3694

View

Where can I find the certificate of calibration for the Rotor-Disc OTV Kit (catalog number 981400)?

FAQ ID - 3695

View

What is the minimum guaranteed shelf-life of the PAXgene Blood ccfDNA Tubes (100) (catalog no. 768115)?

FAQ ID - 3696

View

What methods are used during normalization of RNAseq data in the secondary GeneGlobe Data Analysis Center?

FAQ ID - 3698

View

How do I prepare Buffer MP?

FAQ ID - 3620

View

Are the QIAshredder columns included in various QIAGEN kits the same?

FAQ ID - 3624

View

What is the composition of buffer QBT?

FAQ ID -411

View

What is the composition of buffer QC?

FAQ ID -412

View

What is the composition of buffer QF?

FAQ ID -413

View

What is the composition of buffer STE?

FAQ ID -415

View

What is the composition of buffer TE?

FAQ ID -416

View

What is the composition of buffer FWB2?

FAQ ID -417

View

What is the composition of buffer P3?

FAQ ID -418

View

What is the size, charge and isoelectric point of the DHFR protein in the pQE vectors?

FAQ ID -470

View

Why is carrier RNA used during the isolation of gDNA from microdissected samples with the QIAamp DNA Micro Kit?

FAQ ID -473

View

Why are the centrifugation speeds for the QIAamp DNA Mini kit at 6000 x g? Can I spin at full speed?

FAQ ID -474

View

Are HEPES, MOPS and PIPES buffers compatible with QIAGEN's PhosphoProtein Purification kit?

FAQ ID -482

View

Can Ni-NTA resins be used to purify protein with an internal His-tag?

FAQ ID -496

View

How do I calculate the percentage of silencing with real-time RT-PCR for siRNA?

FAQ ID -498

View

Do you have a protocol for the removal of endotoxins from already purified plasmid DNA?

FAQ ID -500

View

Do you have stability data for genomic DNA isolated with the QIAamp DNA Blood Mini Kit?

FAQ ID -518

View

What is the composition of the siRNA resuspension & annealing buffer?

FAQ ID -522

View

Can Taq DNA Polymerase use RNA as a template, and generate false positives in "no-RT" controls?

FAQ ID -523

View

What is Tissue-Tek O.C.T., and what is it used for?

FAQ ID -530

View

Why do replicates in real-time PCR have different plateau heights?

FAQ ID -539

View

How can I avoid primer-dimer formation during PCR amplification?

FAQ ID -544

View

How can I tell if I have primer-dimers in my PCR reaction?

FAQ ID -552

View

How should I store cDNA produced using Omniscript Reverse Transcriptase?

FAQ ID -561

View

Is it possible to isolate DNA from bone marrow with the QIAamp DNA Blood Kits?

FAQ ID -563

View

What is the composition of Buffer ER? Is it available separately?

FAQ ID -571

View

What can I do when the DNA pellet prepared with QIAGEN Plasmid Kits has been overdried?

FAQ ID -572

View

Do I have to remove the oil from my PCR reaction before using the QIAquick or MinElute PCR Purification Kit?

FAQ ID -575

View

Are the columns of the QIAquick PCR Purification-, Gel Extraction-, and Nucleotide Removal Kit interchangeable?

FAQ ID -577

View

What size is the smallest protein that can be synthesized with the EasyXpress Protein Synthesis Kit?

FAQ ID -600

View

Why do I sometimes get light blue colonies when using the QIAGEN PCR Cloning Kit?

FAQ ID -603

View

What do you recommend for the cleanup of genomic DNA (gDNA)?

FAQ ID -618

View

How can I improve my RNA yield from liver sample when using RNeasy Kits?

FAQ ID -623

View

Why do you recommend using Triton X for the purification of 6xHis-tagged protein?

-100

View

What 96-well plates do you recommend for use with the LiquiChip Workstation?

FAQ ID -630

View

What is a QIAshredder? Is it sufficient for complete disruption and homogenization of my tissue sample?

FAQ ID -631

View

Why does the QIAamp DNA Mini Tissue Protocol require both ATL and AL buffer, while the Blood Protocol only uses AL?

FAQ ID -633

View

Will the QIAquick PCR Purification Kit remove sufficient SYBR Green from real-time PCR reactions to allow sequencing?

FAQ ID -637

View

What is the shelf-life of my QIAGEN Kit?

FAQ ID -651

View

What are the differences between MDA and DOP/PEP methods of Whole Genome Amplification?

FAQ ID -665

View

Can I purchase Phi29 DNA polymerase only?

FAQ ID -708

View

What is the average DNA concentration obtained using the DirectPrep 96 Miniprep Kit?

FAQ ID -721

View

I accidentally stored Buffer RDD of the RNase-Free DNase Set at°C. Will it still function?

-20

View

I ran my RNA out on an agarose gel and can see lots of bands similar to a ladder. Why?

FAQ ID -745

View

How much DNA is obtained in the average PCR reaction?

FAQ ID -750

View

Do you have information about the cleanup of single-stranded DNA (ssDNA) with QIAquick columns?

FAQ ID -759

View

How can I get a certificate for quality assurance (ISO 13485 and ISO 9001) from QIAGEN?

FAQ ID -762

View

What is the difference between Ni-NTA Agarose and Ni-NTA Superflow?

FAQ ID -764

View

What is the composition of Buffer N3?

FAQ ID -767

View

Are the protocols from the BioSprint software compatible with the KingFisher software and vice versa?

FAQ ID -770

View

How should fluorescent labeled probes be stored?

FAQ ID -784

View

Is it possible to use the QuantiTect Reverse Transcription Kit with bacterial RNA?

FAQ ID -785

View

Are Buffer PB of the QIAquick PCR Purification Kit and Buffer QG of the QIAquick Gel Extraction Kit interchangeable?

FAQ ID -786

View

How can I get specific information and a quote for my individual Sequencing Service needs?

FAQ ID -792

View

Can the QIAprep Spin Miniprep Kit be used for isolating plasmid DNA from mammalian cells?

FAQ ID -795

View

How can I separate PCR fragments that are small and very close in size on an agarose gel?

FAQ ID -800

View

Can I reuse the Ni-NTA Agarose and Ni-NTA Superflow resins?

FAQ ID -802

View

What Disinfectant Solution do you recommend for the BioRobot MDx?

FAQ ID -809

View

Is the E. coli Host Strain M15[pREP4] resistant to Zeocin?

FAQ ID -842

View

What annealing temperature should be used with the QuantiTect Primer Assays?

FAQ ID -849

View

Why is it recommended to add 1 mM IPTG for optimal protein yields using the EasyXpress Protein Synthesis Kits?

FAQ ID -860

View

Which QIAGEN plasmid preparation kits will contain LyseBlue Reagent?

FAQ ID -865

View

Do you have an in vitro translation kit allowing the incorporation of seleno-methionine for X-Ray crystallography based protein analysis?

FAQ ID -879

View

Do you have a protocol for the isolation of genomic DNA from frozen clotted blood?

FAQ ID -900. Test

View

Do you have protocols for the isolation of genomic DNA from tissue using QIAGEN-tips 2500 (Mega) and 10000 (Giga)?

FAQ ID -907

View

Do you have a protocol for the isolation of genomic DNA from bone?

FAQ ID -908

View

What do you suggest to prevent degradation of RNA isolated from tissue with high amounts of RNases using RNeasy?

FAQ ID -942

View

Do you have a protocol for Cyanine 570, Cyanine 670, or biotin labeling of cDNA?

FAQ ID -959

View

Do you have a protocol for reverse transfection of adherent cells with siRNA in 384-well plates?

FAQ ID -971

View

Do you have a protocol for the purification of PCR products using the BioSprint System?

FAQ ID -984

View

Do you have a protocol for transient transfection of Hela-S3 cells using PolyFect Transfection Reagent?

FAQ ID -997

View

How can I analyze proteins from exosomes and other extracellular vesicles (EVs) obtained with the exoEasy Maxi Kit by SDS-PAGE?

FAQ ID - 3707

View

How can I increase expression of my 6xHis-tagged protein in E. coli?

FAQ ID -63

View

Can miRNA and other RNAs smaller than 200 nucleotides be isolated with the AllPrep DNA/mRNA Nano Kit?

148723

View

What is the minimum amount of starting material that can be used with the DNA/mRNA Nano Kit?

148724

View

What is the composition of buffer OW2?

FAQ ID -419

View

What is the composition of buffer OBB?

FAQ ID -421

View

Do I need to buy special kits for the QIAcube Connect MDx?

148725

View

What applications are offered on the QIAcube Connect MDx instrument?

148726

View

Can I program my own protocols for the QIAcube Connect MDx?

148727

View

Can purification columns from other suppliers be processed on the QIAcube Connect MDx?

148728

View

What are the dimensions and weight of the QIAcube Connect MDx?

148729

View

Can the QIAcube rotor adapters be autoclaved?

148730

View

Can the QIAcube rotor and/or buckets be removed for cleaning?

148731

View

Can the QIAcube be connected to a laboratory information management system?

148732

View

Are run reports and/or log files available on the QIAcube?

148733

View

How can I get software updates for the QIAcube Connect MDx?

148734

View

Can QIAcube Connect MDx protocol runs be interrupted, and subsequently be continued to completion?

148735

View

Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube?

148736

View

Do I need to discard partially used QIAcube tip racks?

148738

View

How can I decontaminate the QIAcube Connect MDx system?

148739

View

How can I load new protocols onto the QIAcube Connect MDx?

148740

View

Can the QIAcube Connect MDx heater/shaker be used independently from protocol runs?

148741

View

Can the QIAcube Connect MDx centrifuge be used independently from protocol runs?

148742

View

What dedicated QIAcube Kits are available?

148743

View

Can I ask QIAGEN to upgrade my current QIAcube Connect Life Science instrument to make it an MDx instrument?

148744

View

Can I run life science and custom protocols on a QIAcube Connect MDx?

148745

View

What are the heating/cooling rates in the QIAquant instruments?

153919

View

Is a passive internal reference dye, like ROX, required on the QIAquant to obtain reproducible results?

153920

View

What sample volume is suitable for use in the QIAquant?

153921

View

What types of reaction vessels are required for use in the QIAquant?

153922

View

Is the QIAquant equipped with a temperature gradient function?

153923

View

Are the 96- and 384-sample blocks interchangeable?

153924

View

Is it possible to use the touch down PCR option on QIAquant with two acquisition points in the same channel?

153925

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How many molecules can be detected in the system?

153926

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What applications can the QIAquant be used for?

153927

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Can I upgrade my QIAquant 96 2plex instrument to QIAquant 96 5plex or modify the set of color channels?

153928

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Does QIAGEN offers arrays and assays configured specifically for QIAquant instrument?

153929

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Is the software of the QIAquant compatible with 21CFR part11?

153930

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Is it possible to import a standard curve from a previous run?

153947

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Can data be analyzed while the run is proceeding? Can data be analyzed from a previous experiment while another experiment is running?

153948

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Can I use QIAquant 96 Software to analyze experiments carried out on QIAquant 384?

153950

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Can I operate QIAquant 96 instrument using touchscreen and computer simultaneously?

153951

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Is it possible to establish an internet connection with QIAquant instrument?

153952

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Can I connect more than one QIAquant instrument to the same computer?

153953

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What is the algorithm behind data normalization in the QIAquant Software?

153954

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Is it possible to edit the sample sheet after the run in the QIAquant Software?

153955

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What are the LIMS capacity of the software?

153949

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Does the QIAquant Software have the capacity to export data? Which data formats can be used for the export?

153946

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I received a kit containing the MinElute columns; however, they were left out for a while and not stored at 2–8°C upon receipt. Can I still use them?

FAQ ID - 3560

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Is the DNA extracted with the QIAamp DNA FFPE Advanced Kits suitable for downstream applications that require more intact DNA such as long range PCR (>1 kb) and long read DNA sequencing?

153891

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153892

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Can samples be lysed overnight with Proteinase K in the QIAamp DNA FFPE Advanced procedure?

153893

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Can the second Proteinase K lysis step in the QIAamp DNA FFPE Advanced procedure be carried out at lower temperatures than 65°C?

153894

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Can the 2nd Prot K step be omitted?

153896

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What size of DNA can be expected?

153897

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What is the difference of buffer FTB versus Buffer ATL?

153895

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Is it possible to change voltage set-up from 110V to 230V on the QIAcuity instruments?

3761

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Can I see error codes on the instrument touchscreen?

3763

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What is the scope of a regular maintenance of the QIAcuity?

3765

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What is the impact of not applying the latest VPF? Can I reanalyze previously obtained results after installing the latest VPF?

3769

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Can I see on the QIAcuity Software Suite report file if the VPF (Volume Precision Factor) has been used or not?

3770

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Is it necessary to reanalyze a plate with VPF (Volume Precision Factor) that was already processed using a QIAcuity instrument that was purchased in 2020?

3771

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Can I prepare a dPCR reaction directly in QIAcuity Nanoplate?

3774

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How to prepare DNA prior to dPCR?

3778

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Can I use a custom master mix instead of a QIAGEN master mix?

3777

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What are the storage conditions and expiry date of QIAcuity consumables?

3780

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Can I use the QIAcuity Nanoplate in more than one runs?

3781

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What is the VPF (Volume Precision Factor)?

3784

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When and how often do I need a new VPF (Volume Precision Factor)?

3785

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Is a standard curve needed in dPCR?

3786

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Which database versions are used for CRISPR-Q Custom PCR Assay and CRISPR-Q Sanger Primers design?

3788

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Is there anything to consider when processing cultured cells on coated cultivation dishes? Are additional purification steps required to eliminate or reduce coating reagents such as Poly-L-Lysine?

3791

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Is there something to consider when working with transduced cells treated with Polybrene?

3790

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For sequencing application, does the lysate require purification?

3792

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Are the CRISPR-Q PCR Assays and the CRISPR-Q Sanger Primers on GeneGlobe validated?

3793

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Are the PCR products generated with CRISPR-Q Custom PCR Assays only applicable to analysis of editing efficiency by Sanger sequencing?

3794

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Can the new CRISPR PCR Assays be used for dPCR on the QIAcuity? Do you have data or a protocol?

3795

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What is the minimum cell number needed for cell lysis?

3789

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For the Sanger analysis tool, the customer needs to upload two .abi1 files of forward and reverse sequences? Is there any additional info that needs to be provided?

3796

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What is the sequence of the QIAseq miRNA NGS 5’ Adapter?

FAQ ID - 3672

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What are recommended stopping points in the procedure of the EZ1&2 DNA FFPE Kits?

3753

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Are there other options for paraffin removal?

3754

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Can samples be lysed overnight with Proteinase K in the EZ1&2 DNA FFPE procedure?

3755

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Can the second Proteinase K lysis step in the EZ1&2 DNA FFPE procedure be carried out at lower temperatures than 65°C?

3756

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What is the difference between Buffer FTB and Buffer ATL?

3757

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Can the 2nd Proteinase K step be omitted?

3758

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What size of DNA can be expected?

3759

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Is the DNA extracted with the EZ1&2 DNA FFPE Kits suitable for downstream applications that require more intact DNA such as long-range PCR (>1 kb) and long-read DNA sequencing?

3760

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Do you recommend 1-step or 2-step real-time RT-PCR for gene expression analysis?

FAQ ID -1056

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3323 - Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube?

FAQ ID - 3323

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Does salt concentration in the food matter when using the DNeasy mericon Food Kit?

FAQ ID - 3346

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