QIAcuityDx Nanoplates and Accessories

For use with QIAcuityDx digital PCR instruments

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QIAcuityDx Nanoplate 26k 24-well (10)

Cat. No. / ID:   260001

10 QIAcuityDx Nanoplate 26k 24-well, 11 QIAcuity Nanoplate Seals
US$717.00
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Product TypeAccessories
QIAcuityDx Nanoplate
QIAcuity Nanoplate
Nanoplate Seals
Nanoplate Tray
Nanoplate Adapter
The QIAcuityDx Nanoplate 26k 24-well is intended for in vitro diagnostic use.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • QIAcuityDx Nanoplate 26k 24-well
  • Majority of QIAcuity life science configurations supported in Utility mode
  • Up to 8500 or 26,000 partitions per well
  • SBS format

Product Details

Nanoplates are microfluidic digital PCR plates that enable 24 or 96 samples to be run with up to 8500 or 26,000 partitions per well. All four nanoplates are designed to run on QIAcuityDx Four instruments.

In addition, QIAcuity life science configurations across 24 or 96 reactions and varying partitions between 8.5k and 26k may be used within the Utility mode of QIAcuityDx. Note: The IVD mode only allows use of QIAcuityDx plates. 

The different nanoplate configurations can only be used within the QIAcuity dPCR instrument family. In the Utility mode, use the dedicated QIAcuity Nanoplate Adapter when performing automated liquid handling and PCR setup in a QIAcuity nanoplate using the QIAgility. Once done, load the plate onto the QIAcuityDx Four instrument for the dPCR reaction. 

Would you like to learn more about the product and be contacted by one of our dPCR specialists? Contact us for more information and we will get in touch with you shortly.

 

Use of QIAcuityDx Nanoplates and Accessories in Utility and IVD mode

Product Cat. no. Utility mode IVD mode
QIAcuityDx Nanoplate 26k 24-well 260001
QIAcuity Nanoplate 26k 24-well 250001  
QIAcuity Nanoplate 8.5k 24-well 250011  
QIAcuity Nanoplate 8.5k 96-well 250021  
Nanoplate Seals* 250099  
Nanoplate Tray 250098
QIAcuity Nanoplate Adapter 9027242  

*Nanoplate Seals are for use with QIAcuity Nanoplates only.

 

Performance

These are specially designed plates used for digital PCR reactions in QIAcuityDx instruments. We offer four nanoplate types, all in the Society for Biomolecular Screening (SBS) format, but with different specifications for different application needs.

Nanoplate – features and specifications
Type Frame color Specifications Applications
QIAcuityDx Nanoplate 26k 24-well (IVD mode or Utility mode) Red 24-well x approx. 26,000 partitions 
40 µL dPCR reaction per well 
Rare mutation detection, liquid biopsy, pathogen detection, etc.
QIAcuity Nanoplate 26k 24-well (Utility mode) Blue 24-well x approx. 26,000 partitions 
40 µL dPCR reaction per well
QIAcuity Nanoplate 8.5k 24-well (Utility mode) White 24-well x approx. 8500 partitions 
12 µL dPCR reaction per well
Copy number variation analysis, gene expression analysis, NGS library quantification, genome edit detection, etc.
QIAcuity Nanoplate 8.5k 96-well (Utility mode) Gray 96-well x approx. 8500 partitions 
12 µL dPCR reaction per well 

 

Principle

In just 3 simple steps, you can get your dPCR results in approximately 2 hours: pipette and load, run the experiment, analyze results. The principle of the dPCR reaction in the nanoplates is described here.

Procedure

Just like in a qPCR workflow, sample preparation includes the transfer of master mix, probes and primers to a 24- or 96-well nanoplate followed by the addition of samples. The system integrates partitioning, thermocycling and imaging into a single, semi-automated instrument that achieves sample to result in approximately 2 hours. Analysis is performed using the Software Suite. This provides the concentration in copies per microliter of your target sequence when using the Utility mode and can also be used for quality control, such as for positive samples or No Template Control (NTC). This analysis can be extended to remote computers within the same local area network (LAN).

Applications

QIAcuity Nanoplates, in combination with the QIAcuityDx Four and QIAcuityDx Universal MasterMix Kit, enable digital PCR applications, including:

  • Rare mutation detection
  • Copy number variation analysis
  • Gene expression analysis
  • Pathogen detection
  • Genotyping 
  • Cell and gene therapy
  • Residual DNA quantification

 

Supporting data and figures

Resources

Kit Handbooks (1)
Safety Data Sheets (1)
Certificates of Analysis (1)
Latest Software Version (2)

Version 10.1

The Volume Precision Factor (VPF) offers a unique feature to secure precision of concentration results obtained from a QIAcuity dPCR run. 

In general, Nanoplates provide partitions of fixed sizes that enable a very precise way of sample concentration calculation. Potential variation of partition sizes in Nanoplate batches, caused by different microstructure molding forms, can be addressed by applying the batch specific VPF. Furthermore, the VPF includes well-specific volume information and therefore further increases precision of concentration calculation in each well of the Nanoplates.

 

Important note:

The Volume Precision Factor file version 10.1 is compatible with the QIAcuity Software Suite version 2.1.8.23 or higher. All lower versions, namely 1.2.18, 2.0.20, 2.1.7.182, and 2.1.8.20, require a QIAcuity Software Suite patch to be installed prior the upload of VPF version 10.1. Please read the Release Note for QIAcuity Software Suite Volume Precision Factor (VPF) Patches for more information.

If you are using QIAcuity Software Suite versions older than 2.1.8.23, please consider updating your Software Suite and the Instrument Control Software (CSW) to the most recent version prior the upload of VPF version 10.1. After updating to the latest QIAcuity Software Suite software version, no patching is needed. If you are not able to update your QIAcuity Software Suite, please run the patch for your Software Suite version following the instructions provided in the Release Note for QIAcuity Software Suite Volume Precision Factor (VPF) Patches.

After downloading and updating the VPF file within the QIAcuity Software Suite, the VPF is applied automatically to the analysis of a corresponding Nanoplate batch. The VPF file includes information from all available microstructure molding forms and connected Nanoplate batches. It will be stored on the PC where the QIAcuity Software Suite is installed.



 

The QIAcuity backup and restore scripts are a standalone solution to backup all relevant user data of the QIAGEN Software Suite for disaster recovery and to restore the data on a new or existing QIAGEN Software Suite installation. The backup supports the QIAcuity Software Suite version 3.0 and can be conducted manually or automatically by using of the Windows Task Scheduler.

Note: Windows Admin permission is needed to setup and perform an automated backup and for manual backup and restore.

For backup and restore of Software versions lower than 2.5, please refer to the QIAcuity Software Suite Backup and Restore Scripts for version 2.0, 2.1.7, 2.1.8, and 2.2.

For backup and restore of Software version 2.5, please refer to the QIAcuity Software Suite Backup and Restore Scripts for QIAcuity Software Suite 2.5 version 2

Note: Windows Admin permission is needed to setup and perform an automated backup and for manual backup and restore.

Please read the QIAcuity Software Suite Backup and Restore Scripts document for more information and instructions how to use the scripts.

 


Previous Software Versions (1)

The QIAcuity backup and restore scripts are a standalone solution to backup all relevant user data of the QIAGEN Software Suite for disaster recovery and to restore the data on a new or existing QIAGEN Software Suite installation. The backup supports the QIAcuity Software Suite version 2.5 and can be conducted manually or automatically by using of the Windows Task Scheduler.

Note: Windows Admin permission is needed to setup and perform an automated backup and for manual backup and restore.

For backup and restore of Software versions lower than 2.5, please refer to the QIAcuity Software Suite Backup and Restore Scripts for version 2.0, 2.1.7, 2.1.8, and 2.2. 

Note: Windows Admin permission is needed to setup and perform an automated backup and for manual backup and restore.

Please read the QIAcuity Software Suite Backup and Restore Scripts document for more information and instructions how to use the scripts.