PAXgene 96 Blood RNA Kit
高通量纯化全血样品胞内RNA
Products
仅作研究用途。不用于诊断程序。任何表述或表示都并非旨在提供诊断、预防或治疗疾病的信息。
PAXgene 96 Blood RNA Kit (4)
Cat. No. / ID: 762331
4 PAXgene 96 RNA Plates, 4 PAXgene 96 Filter Plates, Buffers, Proteinase K, RNase-free DNase Sets, AirPore Tape Sheets, Collection Vessels. To be used in conjunction with PAXgene Blood RNA Tubes.
PAXgene 96 Incubator Block
Cat. No. / ID: 9238279
Block for denaturation of eluates in PAXgene 96 procedures
特点
- 同步纯化96个样品中的RNA
- 标准化样品处理过程
- 无需有机提取或沉淀
- 纯化获得高品质胞内RNA
产品详情
PAXgene 96 Blood RNA Kit可手工操作高通量纯化胞内RNA。配合PAXgene Blood RNA Tubes（由BD提供，货号762165），系统提供了一个样本分析前处理的标准方案，包括从收集、稳定到RNA纯化的全过程，适用于高通量的临床试验和其他大规模研究课题。
绩效
PAXgene 96 Blood RNA Kit可高通量纯化人全血中的总RNA。配合PAXgene Blood RNA Tubes（BD提供，货号762165），提供从收集、稳定到纯化高品质RNA的完整分析前解决方案。灵敏的PAXgene 96 Blood RNA操作流程重复性好，2.5 ml人全血的一般产量为4–20 µg，将样本间的交叉污染风险降到最低。PAXgene Blood RNA Tubes提供的专有试剂可快速稳定RNA。这种试剂可阻止收集血液后在体外通常会发生的胞内RNA表达谱的剧烈变化（参见" RNA isolation from blood using standard methods"、" Stabilization of gene expression"和" Detection of tyrosinase following blood storage"）。高通量步骤简单、快速、高效，平行处理96个样品可为您在临床实验研究中节约大量时间。
查看图表
原理
在PAXgene 96 Blood RNA System中，血液被直接收集到PAXgene Blood RNA Tubes中，使用PAXgene 96 Blood RNA Kit纯化RNA。PAXgene Blood RNA Tubes的专有试剂，可快速稳定RNA。血液样本在PAXgene Blood RNA Tubes中可室温保存5天。然后使用PAXgene 96 Blood RNA Kit提供的硅胶膜技术纯化RNA。得到的RNA可准确代表体内表达谱，适用于多种下游应用。
程序
血液样本（2.5 ml）收集在PAXgene Blood RNA Tubes（BD提供，货号762165）中，可在室温下保存或转移。在PAXgene 96 Blood RNA操作流程中，RNA的纯化开始于在PAXgene Blood RNA Tube中离心沉淀核酸。沉淀洗涤后加入蛋白酶K消化蛋白。加入乙醇调节结合条件。裂解液加入到PAXgene 96 Filter Plate中，离心去除细胞碎片。裂解液再加入到PAXgene 96 RNA Plate中（参见" PAXgene 96 Blood RNA procedure"）。残留的DNA通过DNase I消化去除。剩余的污染物在3次有效的漂洗步骤中被去除，之后洗脱得到纯化的RNA。
查看图表
应用
从人全血中纯化的RNA可即用于一系列下游应用，包括：
- 核酸扩增技术，如RT-PCR和NASBA
- Expression-array和expression-chip分析
- 定量RT-PCR，包括TaqMan技术
- cDNA合成
- RNase和S1核酸酶保护
- Northern印记、点印记和狭缝印记分析
- 引物延伸
辅助数据和图表
RNA isolation from blood using standard methods.
Blood was collected and RNA was isolated using standard methods (collection in EDTA tubes; no stabilization; RNA isolation using a guanidinium-based method). The graph shows changes in expression of 12 genes after blood collection, measured using real-time RT-PCR. (Data kindly provided by Source Precision Medicine, Boulder, Colorado, USA.)
Specifications
| Features | Specifications |
|---|---|
| Applications | PCR, real-time PCR, qPCR, NASBA, cDNA synthesis |
| Time per run or per prep | <1.5 hours |
| Elution volume | 45–60 µl |
| Main sample type | Whole blood |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Cellular RNA |
| Sample amount | 2.5 ml |
| Stabilization | Yes |
| CE/FDA/IVD compatible | Research use only |
| Format | 96-well plate |
| Technology | Silica technology |
| Processing | Manual (centrifugation or vacuum) |
| Yield | 4–20 µg |
资源
产品介绍与指南 (3)
安全数据表 (1)
试剂盒操作手册 (1)
基因表达分析 (1)
Safety Data Sheets (1)
Certificates of Analysis (1)
FAQ
Where can I find additional information for PreAnalytiX PAXgene products?
What kits does QIAGEN offer to extract RNA from whole blood?
I accidentally stored Buffer RDD of the RNase-Free DNase Set at°C. Will it still function?
How many PAXgene Blood RNA Tubes can be processed per PAXgene 96 Blood RNA Kit well plate?
Can the PAXgene 96 Blood RNA Kit be used for diagnostic or prognostic procedures?
What is the PAXgene 96 filter plate?
What additional equipment is needed to purify RNA using the PAXgene 96 Blood RNA Kit?