连接计算器
此工具确定了在适用于典型连接反应的范围内的各种插入片段:载体摩尔比率所需的插入片段质量。
见解
所需的 DNA 插入片段质量 [g] = 载体 DNA 质量 [g] x {DNA 插入片段长度 / 载体 DNA 长度} x 期望的插入片段与载体摩尔比率
提示：期望的比率为集合 {1, 2, 3, 5 ,7} 中的一个
插入片段长度应小于载体长度
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