QIAamp DSP DNA FFPE Tissue Kit

用于从石蜡包埋组织中纯化DNA

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QIAamp DSP DNA FFPE Tissue Kit (50)

Cat. No. / ID: 60404

For 50 DNA preps: QIAamp MinElute columns, Proteinase K, Buffers, and Collection Tubes (2 ml)

SEK 3,965.00

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数量

QIAamp DSP DNA FFPE Tissue Kit 旨在用于体外诊断用途。

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特点

从石蜡包埋组织中快速纯化DNA
产量高且稳定
去除污染物和抑制剂

产品详情

QIAamp DSP FFPE Tissue Kit利用硅胶技术从福尔马林固定、石蜡包埋组织中纯化DNA。QIAamp DSP DNA FFPE Tissue Kit专为处理福尔马林固定、石蜡包埋组织的实验室设计。

绩效

The QIAamp DSP DNA FFPE Tissue Kit使用QIAamp MinElute离心柱从福尔马林固定、石蜡包埋组织中纯化高品质DNA。可有效从福尔马林固定、石蜡包埋组织切片中纯化基因组DNA，无需过夜孵育。流程可部分去除DNA和福尔马林的交联，可提高产量和DNA品质，用于下游应用。

原理

QIAamp DSP DNA FFPE Tissue Kit使用成熟的QIAamp MinElute技术从福尔马林固定、石蜡包埋组织中纯化基因组DNA。试剂盒包含的硅胶膜可灵活选择20到200 µl的洗脱体积。

特殊优化的裂解条件可从福尔马林固定、石蜡包埋组织切片中高效纯化基因组DNA，无需过夜孵育。蛋白酶K消化后升高温度孵育，可部分去除释放的DNA和福尔马林的交联，提高产量以及DNA品质。从福尔马林固定、石蜡包埋组织中纯化的DNA与从新鲜或冷冻样本中纯化的DNA相比，通常具有更低的分子量。片段化程度取决于样本类型、时间和固定条件。

程序

QIAamp DSP DNA FFPE Tissue Kit使用特殊的裂解条件从组织切片中释放DNA，并避免福尔马林与核酸交联产生的抑制作用。流程包括6个步骤：脱腊、裂解、加热、结合、洗涤和洗脱（参见"Procedure"）。石蜡在二甲苯中溶解并被去除。样本在变性条件下裂解，用蛋白酶K短暂消化。90°C孵育，使福尔马林交联逆转。DNA结合到膜上，污染物被洗去。DNA在Buffer ATE中被洗脱，可即用于扩增反应或在–20°C保存。简便的QIAamp DSP DNA FFPE Tissue Kit操作流程非常适合同时处理多个样本。

应用

QIAamp DSP DNA FFPE Tissue Kit专门用于从福尔马林固定、石蜡包埋组织中纯化DNA。

资源

QIAamp DSP DNA FFPE Tissue Handbook_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices

For the Directive 98/79/EC (IVDD) compliant kit (kit version 1)

High-quality, nucleic acid purification for successful PCR and NGS experiments.

Download Safety Data Sheets for QIAGEN product components.

QIAamp DSP DNA FFPE Tissue Performance Characteristics_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices

High-quality, nucleic acid purification for successful PCR and NGS experiments.

For the Directive 98/79/EC (IVDD) compliant kit (kit version 1)

QIAamp DSP DNA FFPE Tissue Handbook_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices

QIAamp DSP DNA FFPE Tissue Performance Characteristics_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices

FAQ

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

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