Features
- Detects low copies of DNA or cDNA targets
- Produces consistent amplification across a wide dynamic range (6 logs)
- Eliminates non-specific amplification (tested up to 45 cycles)
- Detects molecular targets accurately as fast as 30 minutes
- No loss of activity after eight successive freeze cycles
Product Details
qPCR SG and Probe Mixes are ready-to-use, 2x concentrated master mix of TaqNova HS polymerase, which is a mixture of recombinant Taq polymerase expressed in E. coli and a particular monoclonal anti-Taq antibody.
The TaqNovaHS polymerase enables easy set-up of a hot-start PCR reaction at room temperature. The antibody binds reversibly to the enzyme, inhibiting polymerase activity at ambient temperatures. This prevents the extension of non-specifically annealed primers and primer dimers formed at low temperatures during PCR set-up. The antibody is released from the polymerase during the initial DNA denaturation step, thus providing total DNA polymerase activity during standard cycling conditions.
Performance
| Assay | Specification |
|---|---|
| DNase contamination | None detected |
Principle
Precisely optimized buffer components, reliable DNA polymerase and the appropriate selection of PCR enhancers provide a high performance, sensitivity and specificity over a broad dynamic range and give consistent results across all commonly used real-time PCR platforms.
Procedure
Quality Control
qPCR Mix is extensively tested for its performance in different Real-Time PCR assays. It is free of deoxyribonuclease contamination.
Applications
This is used for applications such as:
- qPCR or real-time PCR
- Gene expression analysis
- Mass screening and pathogen detection
- Characterization of genetically modified organisms (GMO)
Resources
Safety Data Sheets (1)
Certificates of Analysis (1)