QIAamp DSP DNA Blood Mini Kit

从人类全血中纯化基因组DNA

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QIAamp DSP DNA Blood Mini Kit

Cat. No. / ID: 61104

For 50 preps: QIAamp Mini Spin Columns, Buffers, Reagents, Tubes, VacConnectors

QIAamp DSP DNA Blood Mini Kit 旨在用于体外诊断用途。

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QIAamp DSP DNA Blood Mini Kit 旨在用于体外诊断用途。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的（再）订购

特点

通用的DNA纯化系统
灵活的样品采集
与各种抗凝剂和试管兼容
可选离心或真空处理方案

产品详情

QIAamp DSP DNA Blood Mini Kit采用硅胶模技术纯化DNA，适用于进行血液DNA纯化的实验室。

绩效

使用QIAamp DSP DNA Blood Mini Kit纯化的基因组DNA可即用于基于酶扩增或其他修饰的各种下游应用，如PCR。

全血样品使用装有抗凝剂（EDTA或柠檬酸盐）的不同类型的管子收集，包括：BD Vacutainer、Monovette和Vacuette管（参见下表）。血液样品可反复冻融至少3次，仍可用于DNA纯化（参见" DNA from frozen and thawed blood"）。

使用不同的管子收集血液
管子	生产商	编号	体积	200 µl的平均产量
BD Vacutainer 9NC	BD	366007	9 ml	6.4 µg
BD Vacutainer K3E	BD	368457	10 ml	6.6 µg
BD Vacutainer K2E	BD	367864	6 ml	6.4 µg
S-Monovette EDTA	Sarstedt	02.1066.001	9 ml	6.5 µg
S-Monovette CPDA1	Sarstedt	01.1610.001	8.5 ml	6.3 µg
Vacuette K3E	Greiner Bio-One	455036	9 ml	6.5 µg
Vacuette 9NC	Greiner Bio-One	454382	2 ml	6.3 µg

查看图表

DNA from frozen and thawed blood.

原理

QIAamp DSP DNA Blood Mini Kit采用成熟的QIAamp技术纯化基因组DNA。QIAamp硅胶膜特异性结合裂解样品中的DNA，其他裂解物通过离心或真空操作去除。经过有效地洗涤去除污染物，然后洗脱DNA，洗脱体积为50–200 µl。

程序

有两个可供选择的流程用于从血液中纯化基因组DNA，使用离心机或使用真空抽滤装置和离心机（参见" Procedure"）。

含有EDTA或柠檬酸盐的血液样品（200 µl）用含QIAGEN Protease的裂解缓冲液56℃裂解10分钟。乙醇加入到裂解物中，使DNA特异性结合到QIAamp硅胶膜上。裂解物上样到QIAamp Mini离心柱，离心或真空处理。DNA特异性结合到QIAamp硅胶膜上，污染物流走。结合的DNA使用两种不同的缓冲液有效洗涤，离心干缩QIAamp硅胶膜。洗脱缓冲液（50–200 µl）上样到QIAamp硅胶膜，1分钟后离心洗脱纯的DNA。

查看图表

QIAamp DSP DNA Blood Mini Kit procedure.

应用

QIAamp DSP DNA Blood Mini Kit使用经验证的QIAamp技术从新鲜或冷冻的全血中纯化DNA，其中血液是使用柠檬酸盐或EDTA处理过的。

辅助数据和图表

DNA from frozen and thawed blood.

EDTA-treated blood samples were frozen and thawed up to 3 times. DNA was purified at the end of each freeze-thaw cycle. DNA was purified from 200 µl samples using the QIAamp DSP DNA Blood Mini Kit and eluted in 200 µl elution buffer. DNA yields are normalized to the yield from fresh sample (100%). Each bar represents the results from 32 replicates (mean ± standard deviation).

Specifications

Features	Specifications
Applications	PCR, qPCR, real-time RT-PCR, microarray
Main sample type	Whole blood
Elution volume	50–200 µl
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	Genomic DNA
CE/FDA/IVD compatible	CE/IVD
Format	Spin columns
Sample amount	200 µl
Processing	Manual (centrifugation or vacuum)
Technology	Silica technology

资源

QIAamp DSP DNA Blood Mini Performance Characteristics_V3_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices

Download Safety Data Sheets for QIAGEN product components.

QIAamp DSP DNA Blood Mini Handbook_V3_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices

FAQ

It is possible to use a general laboratory vacuum system with the QIAamp DSP Virus Kit. However, this will require validation in-house. The CE-marked QIAamp Kits have been developed, field-tested and validated with the QIAvac 24 Plus vacuum system (QIAvac 24 Plus, QIAvac Connecting System and Vacuum Pump). Using this system with the QIAamp DSP Virus Kit will avoid lengthy validation procedures for the enduser.

FAQ ID -789

When processing samples with the QIAamp DSP DNA Blood Mini Kit and the QIAamp DSP Virus Kit, the vacuum pressure should be below -800 mbar. The QIAvac 24 Plus and the QIAvac Connecting System should be tested according to Appendix B of the QIAvac 24 Plus Handbook before performing a nucleic acid purification procedure.

If you would like to use an equivalent general laboratory vacuum system, make sure that the minimum vacuum pressure is reached. Performance of the QIAamp DSP DNA Blood Mini or Virus Kits in combination with a house-vacuum system has to be validated by the researcher.

FAQ ID -1033

QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. It is particularly suitable for short digestion times. It possesses a high specific activity over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity.

QIAGEN Protease is a broad-specificity Serine protease with high activity, cleaving preferentially at neutral and acidic residues. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of samples.

FAQ ID -761

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12