EZ1 DSP DNA Blood Kit

在EZ1 Advanced或EZ1 Advanced XL工作站上从1–6或1–14个人类生物样本中自动纯化DNA

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EZ1 DSP DNA Blood Kit (48)

Cat. No. / ID: 62124

For 48 DNA preps: Prefilled Reagent Cartridges, Disposable Tip-Holders, Disposable Filter-Tips, Sample Tubes, Elution Tubes

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EZ1 DSP DNA Blood Kit 旨在用于体外诊断用途。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的（再）订购

特点

经CE-marked认证，符合EU IVD Directive 98/79/EC
从全血样本中快速纯化高品质DNA
每次可灵活地纯化1–6或1–14个样本
程序设置简单，只需插卡即可运行
标准化操作流程，确保高重复性结果

产品详情

EZ1 DSP DNA Blood Kit包含所有应用磁珠技术从200或350 µl人类全血中自动纯化DNA所需的试剂和实验室耗材。试剂条中提供有试剂，确保了在EZ1 Advanced或EZ1 Advanced XL工作站上装载试剂的速度和便利性。CE-IVD-marked体系具有卓越性能的规格，能够确保高度可靠的DNA纯化过程。

绩效

高效纯化基因组DNA

自动DNA纯化过程能有高水平的可靠性和标准化程度，并有助于消除因操作失误导致的差异。EZ1 Advanced或EZ1 Advanced XL工作站与EZ1 DSP DNA Blood Kit结合使用能从人类全血中高效纯化基因组DNA。纯化得到的DNA量取决于每个血液样本中白细胞含量（WBC）。EZ1 DSP DNA Blood体系能提供相对较高的DNA产量而与供体的WBC无关。

高品质DNA用于灵敏的特殊分析

灵敏的下游应用需要使用高纯度的DNA以得到可靠的结果。使用EZ1 DSP DNA Blood体系纯化得到的DNA可立即用于多种灵敏的诊断检测，如使用CE-IVD-marked artus PCR Kits进行real-time聚合酶链式反应（PCR）。

高效去除抑制剂

因为PCR由多轮酶反应组成，它比单步酶催化反应对杂质更为敏感。使用EZ1 DSP DNA Blood体系纯化得到的DNA在PCR中有良好的性能，即使使用大体积量洗脱，说明DNA具有高纯度（参见"Eluate volume and PCR performance"）。

原理

自动化的样本制备过程能高效标准地纯化核酸。EZ1 Advanced和EZ1 Advanced XL工作站应用经验证的磁珠技术纯化核酸。试剂的生产过程受到严格的质量控制，确保可靠和稳定的性能。易于使用的仪器、预装并密封的试剂条、简单的程序选择和工作台设置有助于避免操作失误，并使任何人都能操作系统，无论是新手还是专家。将手动操作潜在的传染性样本的过程最小化，确保用户安全和可靠的样本处理。纯化得到的基因组DNA可用于灵敏的下游基于酶扩增反应的诊断检测，如用于基因分型研究的PCR。

程序

EZ1 Advanced或EZ1 Advanced XL工作站与经验证的EZ1 DSP DNA Blood Kit结合使用能从1–6个（EZ1 Advanced工作站）或1–14个（EZ1 Advanced XL）人类全血样本中纯化DNA。所有操作步骤都可在仪器上完成，从穿刺试剂条到洗脱高纯度核酸。该仪器的创新设计使吸头作为分离室使用，增加磁珠纯化的效率并免去离心步骤。精简的操作流程占用最少的手动操作时间，实现稳定的无需人工干预的核酸纯化。

应用

与EZ1 Advanced DSP DNA Blood Card和EZ1 Advanced或EZ1 Advanced XL DSP DNA Blood Card和EZ1 Advanced XL配合使用，EZ1 DSP DNA Blood Kit能够从人类全血中全自动纯化DNA。使用EZ1 DSP DNA Blood Kit纯化得到的基因组DNA可用于基于酶扩增反应的下游诊断分析，如人类白细胞抗原（HLA）和遗传鉴定。

Specifications

Features	Specifications
Elution volume	200 µl
Applications	PCR, real-time RT-PCR, genotyping
Sample amount	350 µl
Technology	Magnetic particles
Main sample type	Whole blood
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	Genomic DNA
CE/FDA/IVD compatible	CE/IVD
For automated processing	BioRobot EZ1
Yield	Varies on WBC count, please see handbook
Processing	Automated

资源

Download Safety Data Sheets for QIAGEN product components.

EZ1 DSP DNA Blood Kit Performance Characteristics_V4_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices

EZ1 DSP DNA Blood Kit Handbook_V4_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices

For the Directive 98/79/EC (IVDD) compliant kit (kit version 3)

New 2D barcodes on EZ1 DSP reagent cartridges

EZ1 DSP DNA Blood Kit Handbook_V4_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices

FAQ

We recommend storing the purified DNA at 2–8°C or at –20°C for up to 24 months. For extended storage time, we recommend storage at –20°C or –80°C for up to 36 months. The impact of DNA stability might be different for the specific downstream application being used and needs to be self-validated by the user.

FAQ ID -1569

Place the reagent cartridges (RCB) into a shaker–incubator, and incubate at 30–40°C with mild agitation for up to 2 hours. Do not use the reagent cartridges (RCB) if the precipitates do not redissolve.

FAQ ID -1568

No. The EZ1 DSP DNA Blood system is a generic system that uses magnetic-particle technology for automated isolation and purification of genomic DNA from human whole blood samples, for in vitro diagnostic purposes. The system is not for use with tissue or stool samples. It is also not for isolation or purification of bacterial, fungal, viral, or parasite nucleic acids, nor for isolation or purification of RNA. The system performance has not been evaluated for isolation or purification of genomic DNA from buffy coat, cultured or isolated cells, swabs, dried blood spots, or cell-free body fluids, such as liquor, plasma, serum, or urine. Contact your local QIAGEN representative for information about other EZ1 Kits.

FAQ ID -1564

For short-term storage (up to 7 days), collect blood in tubes containing EDTA as an anticoagulant and store the tubes at 2–8°C. However, for applications requiring maximum fragment size, such as southern blotting, we recommend storage at 2–8°C for up to 3 days only, as low levels of DNA degradation will occur after this time.

For long-term storage, collect blood in tubes containing a standard anticoagulant (preferably EDTA, if high-molecular-weight DNA is required) and store the tubes at −20°C for a maximum of 4 weeks. Longer storage may be possible depending on the downstream application but needs to be validated by the user.

Yield and quality of the purified DNA may depend on storage conditions of the blood. Fresh blood samples may yield better results. Do not re-freeze blood samples more than 2 times as this may result in decreasing DNA yield.

FAQ ID -1565

No. The EZ1 instruments or the EZ2 Connect MDx are intended to be used only with QIAGEN sample preparation cartridges.

FAQ ID -1567

A sample sheet template is included in the handbook. This sample sheet may be useful for recordkeeping when using the EZ1 DSP DNA Blood or the EZ1 DSP Virus procedure. This sheet can be photocopied and labeled with descriptions of the samples and details of the run.

FAQ ID -1570